high energy compounds
TRANSCRIPT
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HIGH ENERGY COMPOUNDSAND
ATP AS UNIVERSAL ENERGY CURRENCY
PRESENTED BY
SUSHMA P.R 1ST M.Sc BIOTECH,BRINDAVAN COLLEGE
PRESENTED TO
Mrs. DILSHAD BEGUM BIOCHEMISTRY,BRINDAVAN COLLEGE
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Also known as Energy Rich CompoundsCompounds in biological system which on
hydrolysis yield free energy equal to or greater than that of ATP, i.e. ∆ G = -7.3 kcal / mol
Compounds that yield energy less than -7.3 kcal / mol are called Low Energy Compounds.
HIGH ENERGY COMPOUNDS
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Most of the high energy compounds contain phosphate group [except acetyl CoA] hence they are also called high energy phosphates.
The bonds in the high energy compounds which yields energy upon hydrolysis are called high energy bonds.
These bonds are notated by the symbol '~‘ [squiggle].
Fritz Albert Lipmann invented this notation.
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The energy that is actually available [ utilizable ] to do the work is called Free Energy.
Change in free energy is denoted by ∆G. Also known as Gibb’s Free Energy.
For endergonic reactions ∆G will be +veFor exergonic reactions ∆G will be -ve
WHAT IS FREE ENERGY ?
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High energy compounds are mainly classified into 5 groups:
1. Pyrophosphates 2. Enol phosphates 3. Acyl phosphates 4. Thiol phosphates 5. Guanido phosphates or phophagens
CLASSIFICATION OF HIGH ENERGY COMPOUNDS
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The energy bonds in pyrophosphates are acid anhydride bonds.
These bonds are formed by the condensation of acid groups [mainly phosphoric acid] or its derivatives.
An example for pyrophosphates is ATP. It has two high energy diphosphate bonds – phosphoanhydride bonds.
PYROPHOSPHATES
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The bond present here is enolphosphate bondIt is formed when phosphate group attaches to a hydroxyl
group which is bounded to a carbon atom having double bond.
Example : phosphoenolpyruvate
ENOL PHOSPHATES
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An example for acyl phosphate is 1,3- bisphosphoglycerate.
The high energy bond in this compound is formed by the reaction between carboxylic acid group and phosphate group.
ACYL PHOSPHATES
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Here high energy phosphate bond is absent. Instead high energy thioester bond is present.
Thioester bond results from the reaction between thiol and carboxylic acid group’
Example : Acetyl CoA
THIOL PHOSPHATE
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Also known as phophagensThe bond is known as guanidine phosphates bondsIt is formed by the attachment of phosphate group to
guanidine group.Most important compound with this bond is
phosphocreatine.
GUANIDO PHOSPHATES
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Class Bond Example (s)
Pyrophosphates – C – P – P ATP, pyrophosphate
Acyl phosphates O 1,3-bisphospo- ║ glycerate,carbamoyl – C – O ~ P phosphate
Enol phosphates – CH ║ – C – O ~ P PEP
TYPES OF HIGH ENERGY COMPOUNDS
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Thiol esters (thioesters) C Acetyl CoA, ║ Acyl CoA – C – O ~ S –
Guanido phosphates | phosphocreatine – N~ P phosphoargenine
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Compounds ∆Go (kCal/mol) Phosphoenol pyruvate - 14.8Carbamoyl phosphate - 12.3Cyclic AMP - 12.01,3 – Bisphosphoglycerate - 11.8Phosphocreatine - 10.3Acetyl phosphate - 10.3Pyrophosphate - 8.0Acetyl CoA - 7.7ATP→ADP + Pi - 7.3
HIGH ENERGY COMPONDS AND FREE ENERGY RELEASED
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ATP is the most important high energy compound in the living cell.
It contains an adenine group,a ribose sugar and a triphosphate.
ATP is considered as an high energy compound because of the presence of two phospho anhydride bond.
Hydrolysis of the terminal phosphate group yields high negative free energy i.e. -7.3 cal / mol
ATP AS UNIVERSAL ENERGY CURRENCY
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ATP acts as an link between catabolism [exergonic reaction] and anabolism [endergonic reaction].
Catabolic reactions can give energy in the form of ATP.
Anabolic reactions can utilize energy through hydrolysis of ATP.
It transfers phophoryl groups from high energy compounds to less energetic compounds
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HYDROLYSIS OF ATP
Adenosine Pi Pi
-7.3kcal
ADP + Pi
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P P P
Adenosine triphosphate (ATP)
P P P+
Adenosine diphosphate (ADP)
HYDROLYSIS
HIGH ENERGY BOND
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The ATP reaction is commonly written as:
ADP + Pi + energy ATP
The forming of ADP into ATP requires energy (endothermic) – -7.3
kcal/mole
RESYNTHESIS OF ATP
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P P P+
Adenosine diphosphate (ADP)
P P P
Adenosine triphosphate (ATP)
Dehydration[Remove H2O]
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1. ATP – PHOSPHOCREATINE SYSTEM• ATP is resynthesised via phosphocreatine (PC)• PC is stored in muscle cell sarcoplasm
• the following reactions takes place :• PC ---> Pi + C + energy• energy + ADP + Pi ---> ATP• the two reactions together are called a coupled reaction• these reactions are facilitated by the enzyme creatine kinase
• the net effect of these two coupled reactions is :• PC + ADP ---> ATP + C
3 PATHWAYS FOR ATP RESYNTHESIS
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2. THE LACTIC ACID SYSTEM
• This system is an anerobic process and takes place in the sarcoplasm
• The process involves the partial breakdown of glucose – glucose can only be fully broken down in the presence of oxygen.
Only CHO is used in this system• Total= 2 ATP but this is used for resynthesis of ADP to
ATP not muscualr work• the end product of this reaction (in the absence of
oxygen) is lactic acid• the enzyme facilitating the conversion from pyruvic
acid to lactic acid is lactate dehydrogenase (LDH)
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THE AEROBIC SYSTEMSTAGE ONE – GLYCOLYSIS – 2ATPthis takes place in CYTOPLASMand is identical to the lactic acid systemATP regenerated = 2ATP per molecule of glucose
STAGE TWO - KREB’S CYCLE (CITRIC ACID CYCLE) - 2 ATP
occurs in the presence of oxygentaking place in the muscle cell MITOCHONDRIA within the inner
fluid filled matrixpyruvic acid (from glycolysis) promoted by enzymes of the citric
acid cycle, or fatty acids (from body fat) facilitated by the enzyme lipoprotein lipase or protein (keto acids - from muscle) act as the fuel for this stage
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STAGE THREE - ELECTRON TRANSPORT CHAIN – 34 ATP
occurs in the presence of oxygenwithin the cristae of the muscle cell MITOCHONDRIAhydrogen ions and electrons have potential energy which
is released to produce the ATP
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The exergonic hydrolysis of ATP is coupled with the endergonic dehydration process by transferring a phosphate group to another molecule.
For example :
ATP + H2O ADP +Pi
glucose + Pi glucose-6-phosphate + H2O
Overall reaction: glucose+ATP glucose-6-phosphate+ADP
COUPLED REACTION - ATP
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ATP AS A LINK BETWEEN CATABOLIC AND ANABOLIC PATHWAYS
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Metabolism Synthesis e.g. * Polysaccharides * Amino acids * DNA/RNA
Movement Muscle contraction Energy to allow muscle filaments to slide
FUNCTIONS OF ATP
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Active Transport
Changes the shape of carrier proteins
Secretion
In the formation of the lysosomes necessary for exocytosis
Chemical Reactions
A phosphate molecule from ATP can be transferred to . another molecule
Makes it more reactive Lowers activation energy
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WHY ATP IS CONSIDERED AS UNIVERSAL ENERGY CURRENCY?
Common intermediate in many reactions
Links energy requiring and energy producing reactions
• It is universal with all living things
IN SUMMARY
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Easily participates in many reactions
Drives most biological processes
One molecule can be synthesised and perform a large number of jobs
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THANK YOU