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Pauli, C., Grogan, S., Patil, S., Otsuki,

S., Hasegawa, A., Koziol, J., et al.(2011). a!ros!o"i! an#$isto"at$ologi! anal%sis o& $u'an

knee 'enis!i in aging an#osteoart$ritis. OsteoarthritisResearch Society International , 11211*1.

+gna!io, i!$elle Angelika -. io2

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A/stra!t

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Osteoart$ritis in 'enis!i

• A 'enis!us is a "ie!e o&!artilage &oun# w$ere two/ones 'eet (oint s"a!e).enis!i ("lural o&

'enis!us) "rote!t an#!us$ion t$e oint sur&a!ean# /one en#s.

• Osteoart$ritis (OA) is a oint #isease ae!ting t$e

arti!ulate ($%aline)!artilage w$erein t$e to"la%er o& !artilage /reaks#own an# wears awa%allowing /ones un#er t$e

!artilage to ru/ toget$er.

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et$o#olog%

1.2 !' long sli!eswere rese!te# &ro' t$e

anterior, 'i##le an#"osterior region o& ea!$

'enis!us

3+4A5+O678 3i9 &or 2

#a%s

:-H;:A5+O67Al!o$ol

Cltration

S5A+6+6G7H?- &or general

o@er@iew o& tissueorganization

Al!ian lue &or 'u!oi##egeneration

Pi!rosirius e# &or!ollagen >/erorganization

Alizarin e# Stain &or

'enis!us !al!i>!ation

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esults ? :is!ussion

DEGREE OFSEVERITY

OBSERVATIONS

SCO- 0 (6or'al

'enis!us)

- 5$e 'enis!us sur&a!e is s'oot$, no &ra%ing or sur&a!e >/rillation(Fig.2 A-B)

- 6or'al !ell #istri/ution (Fig.3 A)- 6or'al a""earan!e o& e9tra!ellular 'atri9 an# !ollagen >/er

organization (Fig.4 A)

SCO- 1   -  5$e 'enis!us sur&a!e s$ows slig$t >/rillation or un#ulation (Fig.2

C-D)-  :iuse $%"er!ellularit% (Fig.3 B)- :iuse &o!i (C) o& #egenerate# e9tra!ellular 'atri9 ($%alin an#or

'u!oi#), 'ost o& t$e !ollagen >/ers are organize# (Fig.4 B)

SCO- 2   - o#erate >/rillation, &ra%ing an#or un#ulation, so'e !le&ts are"resent (Fig.2 E-F)

- enis!us tissue s$ows $%"o to a!ellular regions (Fig.3 C)

- an#s or !onBuent &o!i o& #egenerate# e9tra!ellular 'atri9su/stan!e ($%alin an#or 'u!oi#), 'ost o& t$e !ollagen >/ers a""earunorganize# (Fig.4 C)

SCO-   -  5$e 'enis!us s$ows se@ere &ra%ing an# tears, se@ere #isru"tion (Fig.2 G-H)

- H%"o!ellular 'enis!us tissue (Fig.3 D)

-3i/ro!artilaginous se"aration (e#e'a, $%aline an#or 'u!oi##egeneration, !%st &or'ation, tears) wit$ unorganize# !ollagen >/ers

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Con!lusion

6ew stan#ar#ize# "roto!ols an# new@ali#ate# gra#ing s%ste's allowe# usto !on#u!t a 'ore s%ste'ati!

e@aluation o& !$anges in aging an# OA'enis!i at a 'a!ros!o"i! an#'i!ros!o"i! le@el. 5$e initiation an#

"rogression o& 'enis!us #egeneration#iers in 'an% as"e!ts &ro' t$ato!!urring in arti!ular !artilage.