histology of female reprod sys

8/13/2019 Histology of Female Reprod Sys

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HISTOLOGY OF FEMALEREPRODUCTION SYSTEM


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Overview

Organ reproduksi wanita

OvariumTuba falopiiUterus

Vagina


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Masa kanak-kanak ovarium blm aktif

Pubertas terjadi perubahan2 pd ovarium,mengakibatkan perub pd tubuh wanita

Pubertas tjd usia 12-16 th ditandai dg perubpd payudara, tumbuhx rambut kemaluan,ketiak dan haid pertama kali (menarche)


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Pubertas masa reproduksi berlangsung 30 th

Masa reproduksi masa klimakterium(peralihan antara masa reproduksi dansenium)

Masa klimakterium haid mjd sedikit danberhenti sama sekali (menopause)


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Ovary- primordial follicle

The ovary consists : outer cortex n innermedulla (zona vaskulosa)

Medulla :connective tissue,muscle cells,elastic n reticular fiber,vesselsFollicles are not present


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cortex :surface epithelium (germinal epithelium)

The surface epith is singel-layered, cells cuboidal orcolumnar

tunica albugeniadoes not contain follicles

cortical stroma

It consists of connective tissue cells, myofibroblasts andinterstitial gland cells and contains primordial folliclesand primary follicles.


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Ovary- primordial follicle

1. Cortical stroma withmany primordial andprimary follicles

2. Ovarian medulla (zonavasculosa)

3. Surface epithelium

4. Tunica albuginea

Semi-thin section; stain: methyleneblue-azure II; magnification: 200


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Ovary Primary Follicle

Secondary Folliclea) and b) Primary follicle

The single-layered epithelium cuboidal or

columnarZona pellucida is interleaved between theoocyte and the follicular epithelium

A basal membrane and a connective tissuesheath (theca folliculi) encase the primaryfollicle


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c) Secondary follicle (preantral follicle)

A multilayered epitheliumThe surface epithelium (follicular epithelium,stratum granulosum) is also called granularepithelium.The zona pellucida : glycoprotein layer.The connective tissue sheath (theca folliculi) followsit.The sheath shows a concentric arrangement ofcells.

d) Atresia of a secondary follicle.


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Ovary Primary Follicle Secondary Follicle

a) and b) Primary follicleSecondary follicle(preantral follicle)d) Atresia of a secondaryfollicle


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Ovary Secondary Follicle or

Preantral Follicle 1 Follicular epithelium2 Zona pellucida

3 Basal membrane4 Theca folliculi

Stain: hematoxylin-eosin;

magnification: 300


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Ovary Secondary Follicle

1 Oocyte2 zona pellucida

3 Multilayered granulosacell epithelium4 Theca folliculi interna5 Interstitial gland cells

Semi-thin section; stain:methyleneblue-azure II;

magnification: 200


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Ovary Tertiary Follicle or

Antral FollicleOvarian follicles with a developed antrum are calledtertiary follicles (antral follicles or Graafian follicles)vessel-free,multilayered granulosa cell epithelium lines theantrum folliculi (1)

a dome-like protrusion in one spot called cumulusoophorus (2)The cumulus oophorus contains the oocyte


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Ovary Tertiary Follicle

1 Antrumfolliculi2 Cumulus oophorus with

oocyte3 Theca folliculi

Stain: hematoxylin-eosinmagnification: 220


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Ovary Graafian Follicle

1 Antrum folliculi2 Cumulus oophorus3 Granulosa epithelial cells

4 Theca folliculi5 Radial corona cells

Stain: alumhematoxylin-eosin;magnification: 25


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Ovary Corpus Luteum

Lipid droplets are present in the granulosa lutein

cells (1)They also store lipid droplets and turn into thecalutein cells (2)They are interspersed with vascularizedconnective tissue (3)


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Ovary Corpus Luteum


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Ovary Corpus Albicans

This creates a shinywhite corpuscle (corpusalbicans sive fibrosum) (1)

The corpus albicans looks like connectivetissue scar


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Ovary Corpus Albicans


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Oocyte

This electron micrograph shows the head region of thespermatozoon in the center (1) Small vesicles (2) occur mostly in the cell centerThe oocyte is enveloped by the zona pellucida (3) ,

which is formed by the glycoproteins of the extracellularmatrix.Cumulus oophorus have already been lostThe light space between zona pellucida and oocyte

surface is the perivitelline space 4

At the time of ovulation, the oocyte has a diameter of120130m. This makes it one of the largest cells in the

human body.


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Oocyte1 Head region of the

spermatozoon2 Vesicle3 Zona pellucida4 Perivitelline space

Electronmicroscopy;

magnification: 1000


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Perkembangan folikel

Folikel primordial folikel primer (oosit dikelilingioleh dua atau lebih sel kuboid)

Setiap siklus menstruasi setelah puberitasfolikel primer perkembangn lbh lanjutpembesaran oosit n proliferasi disekitar folikel selgranulosa

Antara sel granulosa n oosit dibatasi oleh zonapelusida


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Sel granulosa ++proliferasi n folikel +besar rongga berisi liquor folliculi

Oosit terdesak kepinggir dan terdapat ditengahtumpukan sel yg menonjol kdlm rongga folikeldisebut cumulus oophorus

Sementara folikel berproliferasi, sel stroma(sekitar folikel) theca foliculli


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Theca folikuli : theca interna dan theca externa

Theca interna : kaya pemb darah, sintesis

steroid (berdifusi ke folikel) estradiolTheca externa : sedikit pem darah, jar ikatpadat, tdk mensintesis steroid

Terus tumbuhnya 1 folikel primer menyebbfolikel lain atresia


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Setelah ovulasi sisa sel2 granulosa dr folikel corpus luteum (menghasilkan progesteron)

Fertilisasi : Corpus luteum dipertahankan 8mggu awal kehamilanTdk fertilisasi : corpus luteum corpus albicans


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Histofisiologi ovarium

Siklus ovarium tergantung pd :FSHLH

FSH : proliferasi sel granulosa, mengaktifkan enzimu/ sitesis steroid

Theca interna : androgenic steroidprecursors difusi ke folikel estradiol

estradiol FSH LH ovulasi


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Oviduct Ampulla Tubae Uterinae

The human oviduct is about 10 15cm longIt consists of tunica mucosa, tunica muscularis, the

vascularized tela subserosa and the tunica serosa


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The mucosa of the ampulla of the Fallopian tuberises to high longitudinal folds (1)

The tunica muscularis (tuba uterinae musculature)(2) consists of three layers

the inner and outer longitudinal

the middle a circularThe wide subserosal tissue layer containsnumerous vessels (3) (4)


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smooth muscle cells of variable density in thesubperitoneal muscle layer

The tunica serosa (6) covers the subserosaltissue


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1 Mucosa fold (plica)2 Oviduct musculature3 Artery

4 Vein5 Mesosalpinx6 Tunica serosa

Stain: iron hematoxylin-eosin;

magnification: 10


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Oviduct Isthmus Tubae Uterinae

1 Smooth musculature2 Mucosa plicae3 Tunica serosa

4 Subserosal tissue5 Artery

Stain: alumhematoxylin-eosinmagnification: 40


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Uterus Uterine Cervix

Structures mucosa in the uterine cervix n theuterine corpus differencesThe tunica mucosa of the uterine cervix is 2 5mm thick.The plicae of the mucosa (plicae palmatae) (1)create a ragged surface relief

The lamina propria (2) of the cervical mucosa isricher in fibers n stronger than the tunica propriaof the endometrium.


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Uterus Uterine Cervix


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Uterus

The uterus is a pear-shapedIt is about 7 9cm long, 3 4cm wide, 2 3cm thick andweighs 100 120 g.The uterus is located in the plica lata uteri

The uterine cavity (1) (cavum uteri) has the shape of aslitThe uterine wall is 1.5 to 2cm thickStarting at the outer limit :

Perimetrium (2) or tunica serosa (peritoneal epithelium)myometrium (3) or tunica muscularis with four layers(stratum submucosum, stratum vasculosum, stratumsupravasculosum, stratum subserosum)the endometrium (4) or tunica mucosa.


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Uterus Endometrium

Single-layered columnar cells some of them areciliatedfunctional layer endometrium : cellulous connectivetissue with only a few fibers (stroma endometrii)The endometrial stroma resembles mesenchymaltissue called lamina propria contains (nerves andmany vessels, such as the undulating arteries (spiralarteries))

Ut


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Uterus


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Uterus Endometrium

a) Early follicular phase(9th day of the menstrual cycle)

b) Late folliculin phase(16th day of the menstrual cycle)

c) Secretory phase, luteal phase

(23rd day of the menstrual cycle)d) Desquamation phase, menstruation

(1st day of menstrual cycle)


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Uterus Endometrium


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early follicular phase

the tubular uterine glands in the deeper, ap-proximately 5-mm thick functional layer ofthe endometrium show a corkscrewconfigurationThe secretory ducts are empty


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early follicular phase


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final part of the secretion phase

1 Myometrium2 Fibrous connective

tissue3 Sectioned tubular

glandsStain: hematoxylin-eosinmagnification: 80


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Desquamation phase

1 Endometrial basal layer,basalis

2 Myometrium

Stain: hematoxylin-eosinmagnification: 80


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Uterus- myometrium

With a width of 1.5 2.0cm (widest layer of theuterinewall)The thickest layerMostly contain of circular muscle fibers, lymph andblood vessels stratum vasculosumThe inner thin layer stratum subvasculosumThe outer thin layer stratum supravasculosum

Uterus myometrium


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Uterus- myometrium


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Vagina

The wall of the vagina consists :tunica mucosa

does not contain glandsIt is covered by a multilayered nonkeratinizingsquamous epithelium (1)rich in glycogen

tunica muscularistunica adventitia


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The lamina propria (2) with elasticfibers and a venous plexus borders on theepitheliumThe epithelium consists :

basal cellsparabasal cellsintermediary cellssurface cells


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Vagina

1. multilayerednonkeratinizing squamousepithelium

2. The lamina propria


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Vagina- labia minora

They are covered by a multilayered squamousepitheliumThe subepithelial basal cell frequently heavilypigmentedThe loose connective tissue contains collagenfibers

blood vessels, the tissue appears redHair is not presentmany sebaceous glands


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Vagina- labia minora

1 Multilayered stratifiedkeratinizing squamousepithelium

2 Loose connective tissue

3 Sebaceous glands

Stain: azan;magnification: 40


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TERIMAKASIH

histology of female reprod sys

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