history antoni van leeuwenhoek dutch cloth inspector 1684
TRANSCRIPT
- 1822- 1895 French Chemist
“Chance Favors the Prepared Mind”
- Spontaneous Generation 1864
Swan-necked Flask
Ignored Spores
Louis Pasteur
John Tyndall
- Helped Pasteur Twice
1. Tyndall box
Air
2. Tyndallization
Method to sterilize heat sensitive materials
Boil Incubate Boil Incubate Boil Sterile
Koch’s Postulates
• 1. Suspected pathogen always present in diseased animal
• 2. Isolated microorganism in pure culture• 3. Re-inoculate healthy organism with pure
culture and cause disease• 4. Re-isolate the pathogen in pure culture• Began the Concept of “Guinea Pig”?• Lead to Discovery of the Cause of Tuberculosis
Chicken Cholera
• Pasteur at his best?• Attempted Koch’s
postulates• Used old (attenuated)
cultures• Re-inoculates Chicken• Protective “Vaccine”
Pasteur’s Chicken Cholera Experiment
1st Experiment
Healthy Chickens
Old Culture
Fresh Culture
Live Chicken Dead Chickens
2nd Experiment
Attenuated
Vaccinated
Virulent
Rabies Vaccine
• Charles Chamberlain• Filterable “Viruses”• Farmer’s Cure?• Dried Spinal Tissue• Attenuated Virus?• Joseph Meister• First Rabies Vaccine
Antimicrobial Agents
• Paul Ehrlich Agent 606
• Domagk - Prontosil• Alexander Fleming
1929 - Penicillin
Alexander Fleming
History Word ListAntonio van Semmelweis Attenuation
Leeuwenhoek Lister 1867 Chamberlain
1684 Robert Koch Viruses
Spontaneous Anthrax 1876 Alexander
Generation Koch’s Fleming
1864 Postulates 1929
Louis Pasteur Tuberculosis Waksman
Germ Theory Chicken Watson
Lady Montagu Cholera Crick 1953
Jenner 1798 Vaccine 1975