hla-molecules. structure, and functions · 2018. 4. 1. · hla-class i cristalographic structure...
TRANSCRIPT
![Page 1: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/1.jpg)
HLA-Molecules. Structure, and Functions
Joannis Mytilineos MD, PhDDepartment of Transplantation Immunology
Institute for Clinical Transfusion Medicine and Immunogenetics German Red Cross Blood Transfusion Service, and
Department of Transfusion Medicine - University Clinic UlmUlm, Germany
![Page 2: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/2.jpg)
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 3: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/3.jpg)
J. MytilineosEFI/ASHI/APHIA ISS Stintino, 16.09.2013
![Page 4: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/4.jpg)
J. MytilineosEFI/ASHI/APHIA ISS Stintino, 16.09.2013
![Page 5: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/5.jpg)
J. Mytilineos
HLA-Class I Structure
![Page 6: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/6.jpg)
HLA-Class I Cristalographic structure
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 7: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/7.jpg)
J. Mytilineos
HLA-Class II Structure
![Page 8: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/8.jpg)
HLA-Class II Molecule with Peptide
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 9: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/9.jpg)
J. MytilineosEFI/ASHI/APHIA ISS Stintino, 16.09.2013
![Page 10: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/10.jpg)
J. Mytilineos
Inheritence of HLAHLA-A: 01:01 29:01 HLA-A: 02:05 03:01
HLA-B: 08:01 35:03 HLA-B: 08:01 40:01
HLA-Cw: 07:01 04:01 HLA-Cw: 03:03 07:02
HLA-DRB1*: 07:01 03:01 HLA-DRB1*: 13:01 08:01
HLA-DQA1*: 02:01 05:01 HLA-DQA1*: 01:03 04:01
HLA-DQB1*: 03:03 02:01 HLA-DQB1*: 06:03 04:02
HLA-DPB1*: 09:01 04:01 HLA-DPB1*: 04:01 15:01
1 2 3 4
HLA-A: 29:01 03:01 HLA-A: 01:01 03:01 HLA-A: 29:01 03:01 HLA-A: 01:01 02:05
HLA-B: 35:03 40:01 HLA-B: 08:01 40:01 HLA-B: 35:03 40:01 HLA-B: 08:01 08:01
HLA-Cw: 04:01 07:02 HLA-Cw: 07:01 07:02 HLA-Cw: 04:01 07:02 HLA-Cw: 07:01 03:03
HLA-DRB1*: 03:01 08:01 HLA-DRB1*: 07:01 08:01 HLA-DRB1*: 03:01 08:01 HLA-DRB1*: 07:01 13:01
HLA-DQA1*: 05:01 04:01 HLA-DQA1*: 02:01 04:01 HLA-DQA1*: 05:01 04:01 HLA-DQA1*: 02:01 01:03
HLA-DQB1*: 02:01 04:02 HLA-DQB1*: 03:03 04:02 HLA-DQB1*: 02:01 04:02 HLA-DQB1*: 03:03 06:03
HLA-DPB1*: 04:01 15:01 HLA-DPB1*: 09:01 15:01 HLA-DPB1*: 04:01 15:01 HLA-DPB1*: 09:01 04:01
2 4 1 4 2 4 1 3
Mother Father
Child 1 Child 2 Child 3 Child 4
EFI/ASHI/APHIA ISS Stintino, 16.09.2013
![Page 11: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/11.jpg)
J. MytilineosEFI/ASHI/APHIA ISS Stintino, 16.09.2013
![Page 12: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/12.jpg)
Expression of HLA-AntigensHLA-Class I - All nuclated cells
- Platelets- Sperms
HLA-Class II - Makrophages- Monocytes- Aktiv. T-Lymphocytes- B-Lymphocytes
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 13: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/13.jpg)
Biological Functions of the MHC
• Distinction between „self“ and „non-self“• Key Molecules for the Initiation of the
Adaptive, Antigen specific immunologicalResponse against infectious agents
• Ligand for Natural Killer Cells (NK-Cells) • Maturation and Expansion of T- (and B-)
cells
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 14: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/14.jpg)
Antigen Presentation
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 15: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/15.jpg)
Antigen Präsentation
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 16: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/16.jpg)
Vom Gen zur PräsentationHLA-Klasse I
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 17: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/17.jpg)
Vom Gen zur Präsentation HLA-Klasse II
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 18: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/18.jpg)
Impact of Polymorphism in thePeptide Repertoire to bepresented
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 19: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/19.jpg)
J. MytilineosEFI/ASHI/APHIA ISS Stintino, 16.09.2013
![Page 20: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/20.jpg)
Direct and indirect Recognition of Allografts
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 21: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/21.jpg)
T-cell Maturation and Expansion
EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos
![Page 22: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/22.jpg)
B-cell Activation
J. MytilineosEFI/ASHI/APHIA ISS Stintino, 16.09.2013
![Page 23: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/23.jpg)
B-cell Activation
J. MytilineosEFI/ASHI/APHIA ISS Stintino, 16.09.2013
![Page 24: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/24.jpg)
HLA and NK-Cell Interaction
J. MytilineosEFI/ASHI/APHIA ISS Stintino, 16.09.2013
![Page 25: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/25.jpg)
Summary
J. MytilineosEFI/ASHI/APHIA ISS Stintino, 17.09.2013
• HLA-Class I Molecules are Key Molecules forthe Initiation of the ImmunologicalResponse (Infection + Transplantation)
• They act as Ligands for NK-Cells (Infection + Cancer)
• They are crucially involved in the Activationand Maturation process of T- and B-cells
![Page 26: HLA-Molecules. Structure, and Functions · 2018. 4. 1. · HLA-Class I Cristalographic structure EFI/ASHI/APHIA ISS Stintino, 16.09.2013 J. Mytilineos](https://reader036.vdocument.in/reader036/viewer/2022062510/6139c6680051793c8c00abce/html5/thumbnails/26.jpg)
J. Mytilineos
Many Thanks for
your Attention!
EFI/ASHI/APHIA ISS Stintino, 17.09.2013