www.eurofins.comwww.eurofins.comDocument name: File name: EDR: Document owner: Last modified on:

Risiko og analysebehov

makroalger

Holm, Barbro

2018.11.20

2Document name: File name: EDR: Document owner: Last modified on:

Bruksformål makroalger

Source presentation EDR:

Source modification date:

Hvorfor analyserer vi?

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Risiko

Prøveplan – en del av kvalitetssystemet

Krav : at enhver som er ansvarlig for produksjon av mat og fôr vet

hvordan en sikrer at produktet er trygt for konsum for mennesker

og/eller dyr.

• HACCP, Risiko og kvalitetsledelse

• Tilpasset selskapet og til produktet.

• Prøvetakning/analyse til å dokumentere

• Frekvens avhengig av risiko.

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5 Title: Company Presentation Food & Feed Analyses Division

Document name:

Company_Presentation_Food_and_Feed.ppt EDR: 1-F-

MK-PRE-0103614 Document owner: Florian Heupel

Source presentation EDR: 1-F-MK-PRE-0103614

Source modification date: 22/06/2009

Et skifte fra tradisjonell testing

Tradisjonell testing

Contaminants

(e.g. Pesticides,

Mycotoxins, GMO,

Dioxins, Vet. residues)

Nutritional Analyses

Microbiological Analyses

Dagens fokus

Risk Management

Brand Protection

Routine

Analysis

Value added components

Mat & Fôr analyser

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Mikrobiologisk testing

Aerobe mikroorganismer

Koliforme

Mugg

Gjær

Enterococcus

Mat & Fôr analyser

• Proteiner – aminosyreprofil, frie aminosyrer, peptider

• Fett – fettsyreprofil, oksidasjon, lipid klasse

• Karbohydrater – fiber, stivelse, sakkarider, betaglukaner

• Vanninnhold

• Vitaminer

• Mineraler

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Både Kjeldahl og Leco (Dumas) metoden

bestemmer N-innholdet i prøven

Leco finner total nitrogen, Kjeldahl tar ikke med N fra

nitrat og nitritt

Leco gir noe høyere proteinresultat enn Kjeldahl

• Fordeler med Leco:

• Raskere enn Kjeldahl

• Mer miljøvennlig – bruker mindre kjemikalier

Aktuelle analyser

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carotenoids

Vitamin E

Vitamin B1 + B2

Vitamin B3

Vitamin B5

Vitamin B6

Biotin

Vitamin B9

Vitamin B12

Vitamin C

Vitamin K1

Amino acids

Unntak vitamin D2, D3 og

Retinol

Psedocobalamine

Foto NutraIngredients-Asia

Mat & Fôr analyser

Vi analyserer også det som er farlig for oss

• Tungmetaller

• Pestisider

• Pop’s – dioxiner, PCBs

• Veterinære medisinrester

• Allergener

• Migrering fra emballasje

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Mat & Fôr analyser

• GMO

• Opprinnelse

• Innhold

• Autensitet

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Analyses with added values

• Dokumentasjon fra analyser gir merverdi for din bedrift.

Bekyttelse mot matsvindel

• Bygg din merkevare fra

dokumentert kvalitet

• Innhold

• Offentlige rapporter

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Food

fraudSubstitution

Mislabelling

ContaminantDilution

Unapproved

additivesCounterfeiting

Aktuelle arter makroalger for direkte

konsum i Norge

12Document name: File name: EDR: Document owner: Last modified on:

Kilde og figur NIFES

Mikroalger

13Document name: File name: EDR: Document owner: Last modified on:

Kilde European Commission, 2014

Tracing Residues and Contaminants!

Mycotoxins & Natural Plant Toxins

from Aflatoxins to Zearalenone

Organic & Process Contaminants

from Acrylamide to Xylene

Metals and Elements

from Arsenic to Zinc

Veterinary Drug Residues

from Antibiotics to Steroids

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Tung metaller og grunnstoffer

Arsen, Kadmium, Bly, Kvikksølv

– Natrium, Iron, Jod, Selen, Calsium, Sink eller Kobber

• Finnes overalt

➢ Akkumulering av uønskede stoffer i mennesker kan ha negative

helseeffekter.

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16

Pb

Cd

Hg

Cd

Cd

Hg Hg

HgPbCd

HgCd

PbAs

As

As CdPb

Tung metaller – Hvor kommer de fra?

Hg

Heavy metal - Cadmium (Cd)

Sources:

• Used in many technical processes - rechargeable batteries, production of color pigments,

coating of metals to prevent corrosion

• Available in the environment through combustion processes or as part of sewage sludge

• Cadmium is taken up by the plants mainly by the roots from the soil and stored in the

tissue

Toxicology:

• Enrichment in the human body mainly damages kidney

• Inhaled cadmium-containing dusts (tobacco smoke) can cause lung cancer

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Heavy metal - Arsenic (As)

Sources:

• Ubiquitous in soil (cause: volcanic eruptions / burning of fossil fuels such as coal /

petroleum)

• Released by leaching or microbial activities when growing plants in water – absorbed by

the roots of plants (e.g. rice)

• Arsenic may occur as inorganic arsenic as well as organically bound. Inorganic arsenic

compounds are classified as carcinogenic

• Some algae can store arsenic in a larger amount in organically bound form

• Fish and seafood absorb arsenic from the water - organically bound (arsenobetaine and

arsenocholine)

• Rice and rice products may contain relatively high amounts of inorganic arsenic

depending on their geographical origin

Toxicology:

• In the case of organic arsenic compounds, toxicological safety is largely assumed

• Inorganic arsenic compounds are highly toxic and classified as carcinogenic

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ICP-MS/MS - Less is More

Conventional Methods vs. ICP-MS/MS

0.05 0.1 0.5

Limit of Quantification [mg/kg]

0.001

0.001

0.001

0.001

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Metals & Elements – Portfolio

ThUNd

AtPoBiPbTlHgAuPtIrOsReWTaHfRare

Earth

ElementsBaCs

ITeSbSnInCdAgPdRhRuTcMoNbZrYSrRb

BrSeAsGeGaZnCuNiCoFeMnCrVTiScCaK

ClSPSiAlMgNa

FONCBBeLi

H

metal-/ element analysis using ICP-MS, ICP-OES and/or AAS

metal-/ element analysis additionally using ICP-MS/MS

From Arsenic to Zinc – Without Compromise!

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Dioksiner og PCBs

• What are dioxins?

• What are PCBs?

• Why are these

contaminants of

relevance?

• Where do they come from?

• How do they get into food?

• How does dioxin testing

work?

21DIOXINS - An Overview (Status: 26.09.2018)

Exposure pathways of dioxins and PCBs

Waste

incinertation

Chlorine

industry

Therm. industrial

processes

Traffic

exhaust

Sources Food

Beef / cows milk

& -products

Pork meat

& -products

Eggs / poultry meat

& -products

Fish

& -products

Fruits /

vegetables

Environ-

ment

Rain /

deposition

Landfill site /

sewage sludge

Plants / grass

Soil /

water

DIOXINS - An Overview (Status: 26.09.2018) 22

Capacitor

oil

Flame

retardants

Electric

cable

Transformator

oil

Human

PCBs Dioxins

The Parameters

23Company Presentation Eurofins Food Germany

▪ dioxins (PCDD/F)

▪ polychlorinated biphenyls (PCB)

▪ PCB 209

▪ POPs (all 27 Stockholm POPs )

▪ per- and polyfluorinated compounds (PFOS,

PFOA, etc.)

▪ organotin compounds

▪ polycyclic aromatic hydrocarbons (PAHs)

Organochlor Pesticides (OCPs)

▪ brominated flame retardants (PBDE, PBB,

HBCD, TBBPA)

▪ phosphor organic flame retardents

▪ chloroparaffins (SCCP / MCCP)

Main sources of the dioxin intake among

European people

24DIOXINS - An Overview (Status: 26.09.2018)

Source: European Commission, SCOOP Task 3.2.5, June 7, 2000

Milk and dairy products: 16 – 39 %

Meat and meat products: 6 – 32 %

Fish and fish products: 2 – 63 %

Products of plant origin: 6 – 26 %

www.eurofins.de

(NOT) BEING ON FIRE

Halogenated flame retardants

Flammehemmere

Flame Retardants - An Overview (Status: 26.09.2018)

Fire fighting agents versus flame

retardants

Flame Retardants - An Overview (Status: 26.09.2018)

Fire fighting agent

• Used for suppression ofan existing fire

• Role:

• To cool the fire

• To coat the fuelpreventing its contactwith oxygen

Flame retardant

• Added to manufacturedmaterials such asplastics and textiles

• Role:

• To prevent or slow thefurther development ofignition

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Polybrominated Diphenyl ethers (PBDEs)

➢209 different “congeners”

➢Synthetically produced technical mixtures

➢Application as flame retardants in a wide variety of products (e.g. building

materials, electrical and electronic equipment, furniture, motor vehicles, aircraft,

plastics, polyurethane foams and textiles)

➢Sources: flame retardant application, production and recycling of electrical and

electronic devices, leaching from landfills, improper manufacturing processes or

inappropriate disposal of products applied with flame retardants

Under focus: Polybrominated Diphenyl

ethers (PBDEs)

Flame Retardants - An Overview (Status: 26.09.2018)

PBDEsm, n = 1 - 5

EU-legislation

➢ No maximum levels so far for specific categories of food or feed products

➢ Prohibition of use for some technical PBDE-mixtures

➢ Environmental quality standard (EQS) for biota (as marker for water quality)

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Hexabromocyclododecane (HBCDD)

➢Synthetically produced technical compound(s)

➢16 Stereomeres, thereof gamma-HBCDD as technical main product,

alpha-HBCDD as “main metabolite”

➢Application as a flame retardant in thermal insulation foams (expanded

polystyrene foam (EPS), extruded polystyrene foam (XPS)), in HIPS

enclosures, upholstered furniture, car upholstery and insulation blocks in

trucks, packaging material, VTR housing and electrical and electronic

equipment

Under focus – Hexabromocyclododecane

(HBCDD)

Flame Retardants - An Overview (Status: 26.09.2018)

HBCDD

EU-legislation

➢ No maximum levels so far for specific

categories of food or feed products

➢ Environmental quality standard (EQS) for

biota (as marker for water quality)

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Analytical possibilities

Flame Retardants - An Overview (Status: 26.09.2018)

• 24 different congeneres (Tri- to DecaBDE)

• Matrices: food, feed, biota, environmental samples

• Reference method: Internal method (GC-MS)PBDEs

• 7 different congeneres (Tetra- to DecaBB)

• Matrices: food, feed, biota, environmental samples

• Reference method: Internal method (GC-MS)PBBs

• alpha-, beta- and gamma-HBCD

• Matrices: food, feed, biota, environmental samples

• Reference method: Internal method (LC-MS/MS)HBCD

• Matrices: food, feed, biota, environmental samples

• Reference method: Internal method (LC-MS/MS)TBBPA

• 20 different substances

• Validated for biota / suspended particulate matter

• Reference method: Internal method (GC-API-MS/MS)

NBFR andDechloranes

On project-

basis only

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Thank you for your attention

more information at www.dioxine.de

Eurofins GfA Lab Service GmbH – Living your sample

Food&Feed Testing

Norway AS

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Takk for oppmerksomheten!

For mer informasjon

www.eurofins.no

Barbro Holm

BarbroHolm@eurofins.no

+4791182418