host suitability and host-sensitivity of vernonia galamensis accession 17, 18 and 19 to meloidogyne...
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Host-suitability and Host-sensitivity of Vernonia galamensis accession 17, 18 and 19 to Meloidogyne
incognita race 2 under Greenhouse conditions
Matsobane Mathiba
School of Agricultural and Environmental Sciences Department of Soil Science, Plant Production
and Agricultural Engineering
Faculty of Science and Agriculture
10/04/23
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INTRODUCTION
Alternative vegetable oil: Vernonia oil valued for its
vernolic fatty acid
Synthetic epoxies of linoleic and alpha-linolenic
acids are common substitutes of the carcinogenetic
acryllic and phthalate products.
Industrial epoxidation of common fatty acids
increase greenhouse emissions
Faculty of Agriculture and Science-Post-graduate Research Day
10/04/23
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Vernonia galamensis in Limpopo Province
V. galamensis is adaptable to the agronomic
conditions in Limpopo Province, Republic of South
Africa.
Three accessions: 17, 18 and 19, have shown good
adaptations in Limpopo Province and are therefore
being considered as commercial lines.
Root-knot nematodes: Meloidogyne incognita race
2 and M. javanica.
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Three types of host-nematode interactions
Susceptible host: Host allows nematodes to
reproduce and the host suffers yield loss.
Tolerant host: Host allows nematodes to
reproduce, but the host does not suffer yield
loss.
Resistant host: Host does not allow
nematodes to reproduce and there is no yield
loss.
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OBJECTIVE
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To determine Host-suitability and Host-sensitivity of Vernonia galamensis accession
17, 18 and 19 to Meloidogyne incognita race 2 under greenhouse conditions
10/04/23
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MATERIALS AND METHOD
Study area: Greenhouse
Experimental design, treatment and procedure:
Two 5 x 3 Factorial experiments in RCBD with five replicates.
Treatments 0, 500, 1000, 1500 and 2000 nematode densities (Pi)
were inoculated on V. galamensis accession 17, 18 and 19.
Data collection
Nematode numbers (Pf)
Shoot mass
Calculated reproductive factor (Rf) = Pf/Pi
Data analysis
Data: ANOVA and Means: LSD
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RESULTS AND DISCUSSION
Accession 17 Accession 18 Accession 19
Inoculation (Pi)
Root Soil Root Soil Root Soil
500 232.5 2407.8 267.7 2075.4 226.4 834.0
1000 151.7 2069.7 128.0 1409.0 159.0 672.4
1500 81.1 1260.8 82.3 1403.4 73.1 409.2
2000 18.5 876.1 32.2 983.6 32.8 313.4
Accession 17 Accession 18 Accession 19
Inoculation (Pi)
Pf RF Pf RF Pf RF
500 2640.2 5.2a 2343.0 4.7a 1065.2 1.97a
1000 2221.4 2.2b 1531.3 1.5b 831.4 0.83b
1500 1342.0 0.9c 1491.3 1.0bc 482.3 0.32bc
2000 894.6 0.5c 1015.9 0.51c 346.2 0.17c
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10/04/23
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Accession 17 Accession 18 Accession 19
Inoculation (Pi) Shoot
mass (g) Rel*Shoot
mass (g) Rel*Shoot
mass (g) Rel*
Control 35.7a - 37.8a - 41.9a -
500 15.0b - 57.98 26.4b - 30.16 30.9b - 26.25
1000 14.7b - 58.82 29.1b - 23.06 27.6b - 34.13
1500 13.0b - 63.59 29.7b - 21.43 32.8b - 21.72
2000 16.3b - 54.34 27.2b - 28.04 31.5b - 24.82
Relative effect = (treatment/control – 1) 100
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CONCLUSION
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V. galamensis accessions tested are susceptible to M. incognita race 2.
Future research will include screening all available accessions with the aim of grafting the
three commercially used accessions with those with less commercial value but resistant to
nematodes.
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ACKNOWLEDGEMENTS
10/04/23
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