how to bioconjugate-- read solulink's bioconjugation primer
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^ejujiga @ienegbumktieg \riodrBkgukry 0
! ; 848'304'
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k) Jigadrs oust `d ignerperktdf eg `ieoejdnujds ig k oijf*
negtrejjk`jd okggdr
`) Vhd ighdrdgt `iejeminkj ugntieg e thd `ieoejdnujds oust
`d ugkdntdf ktdr oefiinktieg kgf negbumktieg
n) Vhd negbumktieg rdkntieg ennurs firdntjy upeg oixigm thd
twe oefiidf `ieoejdnujds* prddrk`jy get rdquirigm
kffitieg e kg exifkgt* rdfuntkgt* er odtkj
f) Oefiidf `ieoejdnujds krd stk`jd evdr dxtdgfdf pdriefs
d) Ceroktieg e thd nevkjdgt jigakmd is stk`jd ugfdr k rekf
pH rkgmd kgf kt djdvktdf tdopdrkturds
) [ukgtiinktieg e `eth ignerperktdf jigadrs kgf
igkj negbumktd is rdkfijy pdrerodf* i'd* thdy krd
spdntrepheteodtrinkjjy trkndk`jd
m) Negbumktieg ennurs ig `udrdf kqudeus sejutiegs* kt k
physiejeminkj pH
h) ^teinhieodtrinkjjy dinidgt (d'm'* ;2;)
i) Ckst rdkntieg aigdtins
b) Ge ugfdsirk`jd nevkjdgt sifd rdkntiegs furigm oefiinktieg
a) Ge djdntrestktin%hyfrephe`in igtdrkntiegs
j) Jigadrs nkg `d ignerperktdf eg k vkridty e `ieoejdnujds*
ignjufigm ejimes kgf pdptifds* threumh sejif phksd sygthdsis
Vhd njkssinkj odthef usdf te prdpkrd nevkjdgt negbumktds okads
usd e thd okjdioife%thiej neupjigm pkir'
Fiskfvkgtkmds e thd okjdioife%thiej pkir ignjufd2
k) Vhd kqudeus igstk`ijity (hyfrejysis) e thd jigadr ugntiegkj
mreup (i'd'* okjdioifd) eg `ieoejdnujds furigm negbumktieg
`) Vhd gddf te pretdnt thd thiej mreup
n) Vhd sjew aigdtins e thd rdkntieg
f) Vhd gddf te kntivktd thd pretdntdf thiej mreup with k stregm
rdfunigm kmdgt (d'm'* FVV er VND\)
d) Vhd gddf te kntivktd thd pretdntdf thiej mreup `y kffitieg
e k stregm rdfunigm kmdgt (d'm'* FVV)
) Vhd petdgtikj er ugfdsirk`jd heoe/fiodrs vik fisujifd
`rifmd eroktieg
m) Njdkvkmd kgf%er pkrtikj rkmodgtktieg e pretdigs `y
rdfuntieg e fisujifd `egfs
h) Pdfundf er igkntivd pretdig ugntieg
Gdxt
Vhd sejutieg''' v v
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^ejujiga @ienegbumktieg \riodrBkgukry 0
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nhreoepherd k`ser`s kt >46 go with k oejkr dxtigntieg
nedinidgt e 09*' Vhd HyGin%6C@ negbumktieg neupjd' Jimktieg e HyGin kgf 6C@
with kgijigd/nktkjyzdf hyfrkzegd jimktieg `dtwddg 3/hyfrkzigepyrifyj
(HyGin)/ugntiegkjizdf pdptifd 6 kgf dgzkjfdhyfd 4 (jdt) ejjewdf y
TS/SI^ spdntresnepy (>6< go)'
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^ejujiga @ienegbumktieg \riodrBkgukry 0
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\retdig/Ejimegunjdetifd Negbumktieg
Vhd k`ijity te dinidgtjy kgf rdprefuni`jy prdpkrd kgti`efy/
ejimegunjdetifd negbumktds hks jioitdf thdir dxpjeitktieg ig
oujtipjdxdf fikmgestin kssskys'
^ejujigas HyGin%6C@ `ienegbumktieg neupjd* ks kppjidf te
thd negbumktieg e ejimegunjdetifds with kgti`efids* is
steinhieodtrinkjjy dinidgt kgf himh yidjfigm* negvdrtigm 794$
kgti`efy te kgti`efy/ejimegunjdetifd negbumktd (Cimurd =)'
Curthdroerd* negbumktiegs e ejimeodrs e 0/tdroigus e kg ejimegunjdetifd `y k siopjd*
vdry himh/yidjfigm negvdrsieg e k >/ er 4/koige ejime usigm
suje/^/6C@' Kjtdrgktivdjy* k 6C@/phespherkoifitd er firdnt
ignerperktieg eg thd 4/tdroigus nkg `d usdf' ^iopjd oixigm
kgf ignu`ktieg e thd 6C@/ ejimegunjdetifd with k 0> ejf dxndss
e thd HyGin/pdptifd* ejjewdf `y puriinktieg `y fikijtrktieg*
prefunds thd negbumktd ig gdkr qukgtitktivd yidjf'
Cimurd 3' ^trunturds e @en/HGK (;) kgf Coen/Jys/d/(@en/HGK)' Vhdsd rdkmdgts
krd usdf te ignerperktd HyGin mreups eg thd G/ kgf N/tdroigi e pdptifds*
rdspdntivdjy' kgf 6C@/phespherkoifitd is usdf te ignerperktd 6C@ mreups eg
thd 4/tdroigus e ejimegunjdetifds'
Cimurd 4' \dptifd/ejimegunjdetifd negbumktieg' ^F^/\KMD mdj e negbumktds
visukjizdf with TS kna/shkfew' Negbumktieg e twe G/tdroigkj HyGin/
oeficdf pdptifds ;0; kgf ;00 (Jkgds 0 kgf >) rdkntdf with k 4/6C@ oeficdf
;9/odr ejime (Jkgd ;) te yidjf thd pdptifd/ejime negbumktds (Jkgds 6 kgf 4)'
Ks sddg ig jkgds 6 kgf 4* negbumktd eroktieg wks dcnidgt kt k ;2> rdkntieg
steinhieodtry (ejime2pdptifd)' Negbumktd `egf stk`ijity wks tdstdf kt 94EN ig
\@^ er twe hr (Jkgds 3 kgf =)'
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^ejujiga @ienegbumktieg \riodrBkgukry 0
! ; 848'304' ejimegunjdetifds pdr
kgti`efy'
Ig k sdnegf `rdkathreumh er kgti`efy/ejimegunjdetifd
negbumktieg* wd fdvdjepdf k odthef te puriy thd negbumktd
`y kfserptieg te k prepridtkry okmgdtin kigity oktrix thkt
kjjews rdoevkj e dxndss 6C@/ejimegunjdeitfd* ejjewdf `y
djutieg e thd puriidf negbumktd usigm oijf djutieg `udrs'
Vhd evdrkjj yidjf e thd kgti`efy/ejimegunjdetifd negbumktdis >46 go) `is/kryjhyfrkzegd nhreoepherd igte
thd jigadr kro ig `eth `ietig kgf fimeximdgig oefiinktieg
rdkmdgts* thd NhreokJiga @ietig kgf NhreokJigaFimeximdgig (Cimurd 8)' ^pdntrepheteodtrin qukgtiinktieg
e thd ignerperktieg e thdsd nhreoepherin hkptdgs is
strkimhterwkrf kgf himhjy rdprefuni`jd' ^iopjy odksurigm thd
K08< kgf K>46 k`ser`kgnd e thd oefiidf pretdigs yidjfs `eth
thd pretdig negndgtrktieg kgf guo`dr e ignerperktdf jk`djs
(Cimurd =)'
Cimurd =' Kgti`efy/ejimegunjdetifd negbumktieg' Vhd sijvdr stkigdf ^F^/\KMD
mdj prdsdgts fktk er thd negbumktieg kgf puricnktieg e k 6) kgf k 0
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@ieoejdnujd Iooe`ijizktieg
Iooe`ijizktieg e pretdigs* dspdnikjjy kgti`efids* is nritinkjjy
iopertkgt ig okgy `iejeminkj kppjinktiegs1 ignjufigm
ioougeprdnipitktieg* ndjj dgrinhodgt* kgtimdg puriinktieg* kgf
ioougefdtdntieg' Cer this rdkseg* rdsdkrnhdrs etdg rdquird thkt
pretdigs `d iooe`ijizdf eg dithdr k okmgdtin er geg/okmgdtin
surknd'
Vhdrd krd twe okig odthefs usdf te iooe`ijizd pretdigs2 ;)
iooe`ijizktieg e `ietigyjktdf pretdigs eg strdptkvifig/oefiidf
surknds er 0) firdnt nevkjdgt iooe`ijizktieg eg kntivktdf
surknds' ^ejujiga hks dgmigddrdf its negbumktieg nhdoistry te
edr `eth kjtdrgktivds withig thd GkgeJiga kgf OkmgkJiga
koijy e prefunts'
Cer kgti`efy jk`djigm* ^ejujiga fdsimgdf kgf prefundf thd
NhreokJiga @ietig kgf NhreokJiga Fimeximdgig Egd/^het
Aits * whinh ignjufd dvdrythigm rdquirdf te jk`dj* puriy* kgf
qukgtiy ignerperktieg e thdsd jk`djs reo k sigmjd ;
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^ejujiga @ienegbumktieg \riodrBkgukry 0
! ; 848'304' te ;4 tiods mrdktdr `ietig `igfigm nkpknity thkg kgy
ethdr neoodrnikjjy kvkijk`jd okmgdtin `dkf (Cimurds ;
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;' Ok R* Ji [* Kg H dt kj' (083* I' 02084/08='
4' Awegm MK* Pkfu NM* Hwkgm A dt kj' (0
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;4' ]egm NH* Orua FF* Jdd ] dt kj' (0
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^ejujiga @ienegbumktieg \riodrBkgukry 0
! ; 848'304';' ^tdig`drm/Vktokg* M* Huygh O* @kradr F dt kj' (00' Jiu M* Feu ^* Hd B dt kj' (0>' Jiu M* Feu ^* Okrfiressikg M dt kj' (0> I' 423==/38>'
>3' Knhijjds A kgf Aidfrewsai S (0=' Ajigd ON* Fudwdr FJ* Pdfokg B] dt kj' (09' Rhegm X* Jdgm J* @ditig K dt kj' (0* I'
032>64>/>638'
60' Knhijjds A kgf Aidfrewsai MS (0'
6>' Aezjev IK* Odjgya \N* ^treos`erm AD dt kj' (0
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64' Rhegm X* Pdygejfs P* Aiff BP dt kj' (0