how to use a micropipette. when is a micropipette needed? micropipettes are precision instruments...
TRANSCRIPT
How to use a micropipette
When is a micropipette needed?
Micropipettes are precision instruments designed to measure and transfer small volumes of liquids.
Parts of a micropipette
ShaftVolume adjustment dialVolume indicatorPlunger button with two stops
Stop one is used to acquire and then dispense a given volume of liguid
Stop two is to eject the tipAttachment point for a disposable
tip
To acquire a given volume
Set the appropriate volume on the pipette using the dial control.
Attach a tip to the pipette.Push in the plunger button to the
first stop.Insert the tip into the sample.Release the plunger.The tip now contains.
To dispense a given volume
Put the tip into the container where you want the sample.
Press the plunger to the first stop.Remove the plunger from the
container.Point the shaft into the tip waste
container.Push the plunger through the
second stop to eject the tip.
Now its your turn to practiceMicropipette challenge and Practice gel loading