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Howard Junca, Ph.D. URL: www.howardjunca.com Email: info@howardjunca.com Address: CL 19 5A 64 Chia, Colombia Phone: +57 3002335295 Skype: howard_junca www.microbiomas.org Personal information Full name: Howard Armando Junca Díaz ID & Citizenship: CC 79.785.003, Colombian Date and place of birth: July 4th, 1975, Bogota, Colombia Marital status: Married (2000), two daughters (born in 2005 & 2007) Based in Colombia since: 01.03.2008 | ResearchGate: https://www.researchgate.net/profile/Howard_Junca | Google Scholar: http://scholar.google.com.co/citations?user=2EKXqTsAAAAJ&hl=en&oi=ao Education & Professional Experience 01.20.1992-03.20.1997 Microbiologist, (“Diploma”), 1992-1997, Department of Microbiology, Faculty of Biological Sciences, Universidad de los Andes, Bogotá, Colombia. 15.11.1998- 07.03.2002 Biologist, 2002 (Biólogo 2° ciclo), 1998-2002, Ministry of Science and Education and Universidad Complutense, Madrid, Spain. 03.11.1998-02.10.2001 M.Sc. Genetics, 2002, (“Suficiencia Investigadora”), Department of Genetics, Faculty of Biological Sciences, Universidad Complutense, Madrid, Spain & Master in Environmental Impact Assessment, Institute of Ecological Research, Málaga, Spain. Thesis awarded with honours. 05.02.2001-27.05.2004 Ph.D. in Biological Sciences, 2004, Thesis defence date: May 27th 2004 („Doktor der Naturwissenschaften, Dr. rer. nat.“), Department of Microbiology. TU-Brunswick, Germany (Gemeinsame Naturwissenschaftliche Fakultät, Technischen Universität Carolo-Wilhelmina zu Braunschweig). Doctoral thesis awarded with “Summa Cum Laude”. Full text available to download at: http://opus.tu-bs.de/opus/volltexte/2005/799/

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28.05.2004-06.07.2006 Postdoctoral Research Scientist, AG Biodegradation, German Research Centre for Biotechnology (Gesellschaft für Biotechnologische Forschung) - GBF. Braunschweig, Germany. 07.07.2006-29.02.2008 Postdoctoral Federal Research Scientist, 2006-2008. RG Microbial Interactions and Processes. Helmholtz-Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research - Helmholtz Society) | HZI. Braunschweig, Germany. 15.08.2008-31.12.2009 Research Associate. Research Groups Molecular Biotechnology & Research Group Microbial Genetics, CorpoGen, Bogota DC, Colombia. Member of CorpoGen Executive Board since 2008. Associate Scientist, Center of Research Excellence “Colombian Center of Genomics and Bioinformatics of Extreme Environments – GeBiX", Bogotá, Colombia. 03.04.2011-31.06.2012 Adjunct Professor, Microbiology, Molecular Biology and Omics, Institute for Food Science and Technology (ICTA), Universidad Nacional de Colombia, Sede Bogotá. 01.01.2010-15.12.2015 Researcher & Head of Research Group | CorpoGen, Bogota DC, Colombia. 13.01.2013-15.07.2015 Full Professor (Profesor Titular), Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Bogotá. Main activities: 1) Coordinator of the MSc program in Applied Biology 2013; 2) Director of Research Center of the Faculty of Basic and Applied Sciences 2014-2015; 3) Coordinator of Molecular and Cellular Biology Area 2014; 4) Member of the Committee for the design of the PhD Program in Science and Engineering at UMNG 2013-2015; 5) Member of the Committee of Postgraduate Programs of the Faculty 6) Member of the Faculty Council; 7) Member of the University Committee for Research and Innovation. 8) Teaching duties: Lecturer since 2013 of the practical and theoretical courses of the Applied Biology Program: a) Biochemistry 4403 2013-2014; b) Applied Microbiology 4409. 2013-2015. https://sites.google.com/a/unimilitar.edu.co/howard-junca/ 16.12.2015-present Founder & Director, Head of Research Group Microbial Ecology: Metabolism, Genomics & Evolution; Microbiomas Foundation, Ecogenomics and Holobionts. www.microbiomas.org Interests Bioremediation Biochemistry & Genetics., Environmental Microbiology and Biotechnology, New Sequencing Technologies and Microbial Ecology, Molecular Phylogenetics, DNA Barcoding and Genomics, Metagenomics, Microbiomes, Holobionts, Aromatics Biotransformation, Metabolic Gene Diversity, Microbial Physiology and Microbial Metabolism, Protein Engineering, Biocatalysis, Bioprocesses and Environmental DNA Microarrays, Biomarkers, Bioactives & Natural Products, Biological Screening Systems

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Main research lines Ecogenomics of bioremediation processes | Metabolism and biotechnology of microbiomes at extreme environments and in marine holobiont asssociations | Functional enzimatic and bioactive exploration of microhabitats by screening of metagenomic libraries | Development and adaptation of omic technologies for the study of microbial communities | Microbial diversity and environmental biotechnology | Development of tools for biological screening, production and valorization of natural bioactives, biomarkers and bioprocesses. Research projects, role, funding institution, and partners involved (2010-2015) 31.09.2013-31-09.2015 Coinvestigator of national project (Colciencias): Genomic, microbiome and climatic study of the pathogenic nature of diseased Rachycentron canadum (Cobia) cultures at the Caribbean Sea (CorpoGen & Ceniacua) 11.12.2010-10.12.2012 Principal Investigator of national project (Colciencias): Metagenomic exploration of the microbial communities associated to marine sponges: searching for the ancient and natural origin of the potential to generate/degrade halogenated aromatics (CorpoGen) 31.11.2010-31.12.2014 Principal Investigator (PI) of national project (Colciencias): Platform for exploring antimalarial compounds in metagenomes (CorpoGen; Universidad Nacional de Colombia - Sede Medellín and CRESIB, Catalunya, Spain). 02.06.2012-02.12.2012 Principal Investigator (PI) of national project (Fundación para la Promoción de la Investigación y la Tecnología FPIT - Banco de la República, Colombia): Genome sequencing of a Bacillus strain pathogenic for shrimp aquaculture “Secuenciación y análisis del genoma del patógeno emergente Bacillus sp., cepa CENIACUA-CG01, responsable de brotes de mortalidades severas en produccciones de L. vannamei (camarón blanco)“(CorpoGen and Ceniacua) 16.04.2012-15.10.2012 Principal Investigator (PI) of international-funded project (Biodiversity Institute of Ontario, IDRC- Canada): "First DNA barcoding sampling survey of processed meat and fish derived-products commercialized in Bogota, Colombia: assessing the reliability of the marketed identity in meat food products for human consumption, their potential origins and related risks for health, food security and ecosystem preservation in the country." (CorpoGen and Universidad Nacional de Colombia, Sede Bogotá) +Dissemination in national news and media. Article in Semana Sostenible: http://sostenibilidad.semana.com/impacto/articulo/fraude-comercializacion-pescado-gato-liebre-basa-robalo/33081 16.04.2010-16.04.2014 Partner of the European Commission research consortium project (FP7): MAGICPAH - Molecular Approaches and MetaGenomic Investigations for optimizing Clean-up of PAH contaminated sites www.magicpah.org (scientific network of 15 research institutes in 9 countries) 15.09.2008-31.11.2014

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Participant in the national network project (Colombian Excellence Research Center GeBiX-Colciencias): Establishing a metagenomic and bioinformatic platform for characterizing and using genetic resources of extreme environments www.gebix.org.co (14 research labs in 7 national institutions) 01.02.2011-28.12.2012 Colombian partner on the collaborative research project with the Center for Biotechnology, Universidade Federal do Rio Grande do Sul. Project funded under the bilateral official agreement between Brasil (CNPq) and Colombia (Colciencias) 15.02.2014-15.02.2015 Principal Investigator National Project (UMNG) Establishing a collaborative network for holobiont exchange for microbiomes investigations (UMNG; UNICE, France; IMBE, France; MRC, Monaco) 13.10.2014-13.10.2016 Colombian coordinator in the binational researchers exchange project: Ecos Nord (France) and Colciencias, Colombia (IMBE, Marseille; Université Sophia Antipolis, Nice, France, Universidad Nacional de Colombia, Sede Bogotá & CorpoGen) 12.10.2010-06.06.2014 Colombian participant in the binational researchers exchange project 2010-2014: Ecos Nord (France) and Colciencias, Colombia (Université Sophia Antipolis, Nice, France, Universidad Nacional de Colombia, Sede Medellín; CorpoGen) Alumni - Supervised students (only postgraduate MSc & PhD theses) 2012-: Director of PhD thesis (student: Victor Manuel Tibatá), Doctoral Program in Veterinary Medicine, Universidad Nacional de Colombia, Sede Bogotá. 2013-: Director of PhD thesis (student: Erika García), Doctoral Program in Biology, Universidad Nacional de Colombia, Sede Bogotá, Colombia. 2010-2014: Director of PhD thesis (student: Álvaro Monguí), Doctoral Program in Biological Sciences, Universidad de los Andes, Bogotá. Finished 2012-2014: Director of MSc thesis (student: Marcela Villegas), MSc Programme in Biological Sciences, Universidad de los Andes, Colombia. Finished 2012-2014: Director of MSc thesis (student: Francy Pérez), MSc Program in Biological Sciences, Universidad de los Andes, Colombia. Finished 2010-2012: Director of MSc thesis (student: Eric JC Gálvez), MSc Programme in Biological Sciences, Universidad de los Andes, Colombia. Finished

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Editorial posts & distinctions Reviewer of >70 manuscripts submitted to 15 international journals with ISI impact factors> 2. Trends in Biotechnology (Elsevier), FEMS Microbiology Ecology (under Elsevier or Blackwell), Mycological Research (Cambridge Journals), FEMS Microbiology Letters (under Elsevier or Blackwell), Applied Biochemistry and Biotechnology (Springer), Bulletin of Marine and Coastal Research (Invemar), Journal of Applied Microbiology (Blackwell Publishing), Environmental Microbiology (Blackwell Publishing), Journal of Environmental Quality (ASA, CSSA, and SSSA), Bioresource Technology (Elsevier), European Journal of Soil Biology (Elsevier), Microbiology-SGM (Society of General Microbiology, UK) Guest Editor for Frontiers Research Topic Microbial Ecogenomics: Elucidation of key community structures and functions in bioremediation processes in situ. www.frontiers.org | http://goo.gl/IQmTm of Invited Coeditor for section Environmental Biotechnology 2014 of Current Opinion in Biotechnology, Elsevier http://www.sciencedirect.com/science/journal/09581669 (5-Year Impact Factor: 8.934). First Latin American Invited as Editor for a volume of the Current Opinion Series. Reviewer of academic research project proposals submitted to funding agencies and national institutions in Colombia (Colciencias, Republic Bank Foundation, Universidad de los Andes, Universidad Javeriana, Universidad Nacional de Colombia, among others) and international (from countries like Germany, Uruguay, Chile, Brazil). Associate Faculty Member, Faculty1000 (f1000prime), network for post-publication recommendation and peer review of biomedical literature. First Latin American included in the microbiology area: http://f1000.com/prime/thefaculty/microbiol/environmental Senior Researcher “Investigador Senior” National Ranking of Scientist in Colombia- Colciencias since 2014 & Position 118 among the most cited Colombia-based scientist (including all scientific areas/disciplines) 2015: www.webometrics.info/en/node/70 Cofounder (2009) and President (2014-2018) of the Colombian Society for Microbiology Asociación Colombiana de Microbiología - ACM www.microbiologia.co President 2012-2014 and current Vice-president 2014-2016 of the Latin American Association for Microbiology ALAM. Co-organizer with Universidad de Antioquia of the XXII Latin American Congress of Microbiology ALAM 2014 www.microbiologialatinoamericana.org +1500 participants and +1000 presentations. www.microbiologialatinoamericana.org

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Personal references Strong links and connections with a wide range of industrial, governmental and academic sectors and professionals in Colombia and abroad (full list per request). Selection of main international research collaborators and personal references: Prof. Dr. Dietmar H. Pieper, Helmholtz Center for Infection Research HZI, Germany Prof. Dr. Victor de Lorenzo, CNB CSIC, Spain. Prof. Dr. Alexandre J. Macedo, Center for Biotechnology, UFRGS, Brazil Prof. Dr. Michael Seeger, Dr. Beatriz Cámara, U. Fed. Santamaría, Valparaíso, Chile Dr. Luis Rojas Araya, Director CICITEM, Chile Prof. Dr. Maria Brennerova, CAS, Czech Republic Prof. Dr. Alfons Stams, U, Wageningen, Netherlands Prof. Dr. Hermann Heipieper, UFZ Leizig, Germany Prof. Dr. Newton Gomes, U de Aveiro, Portugal Dr. Thierry Perez, IMBE, Marseille, France Prof. Dr. Olivier P. Thomas, U. of Galway, Ireland Publications in international peer-reviewed journals | ResearchGate: https://www.researchgate.net/profile/Howard_Junca | Google Scholar: http://scholar.google.com.co/citations?user=2EKXqTsAAAAJ&hl=en&oi=ao

*Only articles indexed by ISI Thompson Scientific and Scopus • Spirochaetes dominate the microbial community associated with the red coral Corallium rubrum on a broad geographic scale. van de Water JA, Melkonian R, Junca H, Voolstra CR, Reynaud S, Allemand D, Ferrier-Pagès C. 2016. Scientific Reports. Accepted. In press. • Draft Genome Sequence of Bacillus licheniformis CG-B52, a Highly Virulent Bacterium of Pacific White Shrimp (Litopenaeus vannamei), Isolated from a Colombian Caribbean Aquaculture Outbreak. Gálvez EJ, Carrillo-Castro K, Zárate L, Güiza L, Pieper DH, García-Bonilla E, Salazar M, Junca H. Genome Announcements. 2016 May 12;4(3). pii: e00321-16. doi: 10.1128/genomeA.00321-16. PMID: 27174263 • Voices of biotech. Amit I, Baker D, Barker R, Berger B, Bertozzi C, Bhatia S, Biffi A, Demichelis F, Doudna J, Dowdy SF, Endy D, Helmstaedter M, Junca H, June C, Kamb S, Khvorova A, Kim DH, Kim JS, Krishnan Y, Lakadamyali M, Lappalainen T, Lewin S, Liao J, Loman N, Lundberg E, Lynd L, Martin C, Mellman I, Miyawaki A, Mummery C, Nelson K, Paz J, Peralta-Yahya P, Picotti P, Polyak K, Prather K, Qin J, Quake S, Regev A, Rogers JA, Shetty R, Sommer M, Stevens M, Stolovitzky G, Takahashi M, Tang F, Teichmann S, Torres-Padilla ME, Tripathi L, Vemula P, Verdine G, Vollmer F, Wang J, Ying JY, Zhang F, Zhang T. Nature Biotechnology. 2016 Mar 10;34(3):270-5. doi: 10.1038/nbt.3502. PMID:26963549 • Microbial Toluene Removal in Hypoxic Model Constructed Wetlands Occurs Predominantly via the Ring Monooxygenation Pathway. Martínez-Lavanchy PM, Chen Z, Lünsmann V, Marin-Cevada V, Vilchez-Vargas R, Pieper DH, Reiche N, Kappelmeyer U, Imparato V, Junca H, Nijenhuis I, Müller JA, Kuschk P, Heipieper HJ. Applied and Environmental Microbiology. 2015 Sep;81(18):6241-52. doi: 10.1128/AEM.01822-15. Epub 2015 Jul 6. PMID: 26150458 • Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries. Mongui A, Pérez-Llanos FJ, Yamamoto MM, Lozano M, Zambrano MM, Del Portillo P, Fernández Becerra C, Restrepo S, Del Portillo HA, Junca H. Malaria Journal. 2015 Jun 4;14:233. doi: 10.1186/s12936-015-0748-6. PMID: 26040274

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• Distinct composition signatures of archaeal and bacterial phylotypes in the Wanda Glacier forefield, Antarctic Peninsula. Pessi IS, Osorio-Forero C, Gálvez EJ, Simões FL, Simões JC, Junca H, Macedo AJ. FEMS Microbiology Ecology. 2015 Jan;91(1):1-10. doi: 10.1093/femsec/fiu005. Epub 2014 Dec 5. PMID: 25764530 • AromaDeg, a novel database for phylogenomics of aerobic bacterial degradation of aromatics. Duarte M, Jauregui R, Vilchez-Vargas R, Junca H, Pieper DH. Database (Oxford). 2014 Dec 1; 2014. pii: bau118. doi: 10.1093/database/bau118. Print 2014. PMID: 25468931 • Neotropical Andes hot springs harbor diverse and distinct planktonic microbial communities. Delgado-Serrano L, López G, Bohorquez LC, Bustos JR, Rubiano C, Osorio-Forero C, Junca H, Baena S, Zambrano MM. FEMS Microbiology Ecology. 2014 Jul;89(1):56-66. doi: 10.1111/1574-6941.12333. Epub 2014 Apr 29. PMID: 24689975 • Editorial overview: environmental biotechnology. Harms H, Junca H. Current Opinion in Biotechnology. 2014 Jun;27:vii-ix. doi: 10.1016/j.copbio.2014.04.002. Epub 2014 Apr 26. PMID: 24780846 • Genome analysis and physiological comparison of Alicycliphilus denitrificans strains BC and K601(T.) Oosterkamp MJ, Veuskens T, Talarico Saia F, Weelink SA, Goodwin LA, Daligault HE, Bruce DC, Detter JC, Tapia R, Han CS, Land ML, Hauser LJ, Langenhoff AA, Gerritse J, van Berkel WJ, Pieper DH, Junca H, Smidt H, Schraa G, Davids M, Schaap PJ, Plugge CM, Stams AJ. PLoS One. 2013 Jun 25;8(6):e66971. doi: 10.1371/journal.pone.0066971. PMID: 23825601 • Analysis of the microbial gene landscape and transcriptome for aromatic pollutants and alkane degradation using a novel internally calibrated microarray system. Vilchez-Vargas R, Geffers R, Suárez-Diez M, Conte I, Waliczek A, Kaser VS, Kralova M, Junca H, Pieper DH. Environmental Microbiology. 2013 Apr;15(4):1016-39. doi: 10.1111/j.1462-2920.2012.02752.x. Epub 2012 Apr 20. PMID: 22515215 • In-depth characterization via complementing culture-independent approaches of the microbial community in an acidic hot spring of the Colombian Andes. Bohorquez LC, Delgado-Serrano L, López G, Osorio-Forero C, Klepac-Ceraj V, Kolter R, Junca H, Baena S, Zambrano MM. Microbial Ecology. 2012 Jan;63(1):103-15. doi: 10.1007/s00248-011-9943-3. Epub 2011 Sep 27. PMID: 21947461 • Structural and functional insights from the metagenome of an acidic hot spring microbial planktonic community in the Colombian Andes. Jiménez DJ, Andreote FD, Chaves D, Montaña JS, Osorio-Forero C, Junca H, Zambrano MM, Baena S. PLoS One. 2012;7(12):e52069. doi: 10.1371/journal.pone.0052069 • Gulosibacter molinativorax ON4T molinate hydrolase, a novel cobalt-dependent amidohydrolase. Duarte M, Ferreira-da-Silva F, Lünsdorf H, Junca H, Gales L, Pieper DH, Nunes OC. Journal of Bacteriology. 2011 Oct;193(20):5810-6. doi: 10.1128/JB.05054-11. PMID: 21840982 • Evaluating a nested-PCR assay for detecting Streptococcus agalactiae in red tilapia (Oreochromis sp.) tissue. Jimenez A, Tibata V, Junca H, Ariza F, Verjan N, Iregui C. 2011. Aquaculture. 320(3):341-346. • Genome sequences of Alicycliphilus denitrificans strain BC and K601T Oosterkamp MJ, Veuskens T, Plugge CM, Langenhoff AA, Gerritse J, van Berkel WJ, Pieper DH, Junca H, Goodwin LA, Daligault HE, Bruce DC, Detter JC, Tapia R, Han CS, Land ML, Hauser LJ, Smidt H, Stams AJ. 2011. Journal of Bacteriology. 193(18):5028-5029. • Metabolic networks, microbial ecology and 'omics' technologies: towards understanding in situ biodegradation processes. Vilchez-Vargas R, Junca H, Pieper DH. 2010. Environmental Microbiology. 12(12):3089-3104. • Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia. Gomes NC, Flocco CG, Costa R, Junca H, Vilchez R, Pieper DH, Krögerrecklenfort E, Paranhos R, Mendonça-Hagler LC, Smalla K. 2010. FEMS Microbiology Ecology. 74(2):276-290 • Metagenomics reveals diversity and abundance of meta- cleavage pathways in microbial communities from soil highly contaminated with jet fuel under air sparging bioremediation. Brennerova M, Josefiova J, Brenner V, Pieper DH, Junca H. 2009. Environmental Microbiology. 11(9):2216-2227. • Characterization of a gene cluster involved in 4- chlorocatechol degradation by Pseudomonas reinekei MT1Cámara B, Nikodem P, Bielecki P, Bobadilla R, Junca H, Pieper DH. 2009. Journal of Bacteriology.

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191(15):4905-4915. • Growth of Pseudomonas chloritidismutans AW-1T on n-alkanes with chlorate as electron acceptor. Mehboob F, Junca H, Schraa G, Stams AJM. 2009. Applied Microbiology & Biotechnology. 82(3):565-577. • Enhancement of the microbial community biomass and diversity during air sparging bioremediation of a soil highly contaminated with kerosene and BTEX. Kabelitz N, Machackova J, Imfeld G, Brennerova M, Pieper DH, Heipieper HJ, Junca H. 2009. Applied Microbiology & Biotechnology. 82(3):565-577. • Isolation and characterization of Alicycliphilus denitrificans strain BC that grows on benzene with chlorate as electron acceptor. Weelink SAB, Tan NCG, ten Broeke H, van den Kieboom C, van Doesburg W, Langenhoff AAM, Gerritse J, Junca H, Stams AJM. 2008. Applied & Environmental Microbiology. 74(21):6672-6681. • trans-Dienelactone hydrolase from Pseudomonas reinekei MT1, a novel zinc-dependent hydrolase. Cámara B, Marin M, Schlömann M, Hecht H-J, Junca H, Pieper DH. 2008. Biochemical & Biophysical Research Communications. 376(2):423-428. • Two angular dioxygenases contribute to the metabolic versatility of the dibenzofuran degrading Rhodococcus sp. strain HA01. Aly HA, Huu NB, Wray V, Junca H, Pieper DH. 2008. Applied & Environmental Microbiology. 74(12):3812-3822. • Melanoidin-containing wastewaters induce selective laccase gene expression in the white-rot fungus Trametes sp. I-62. González T, Terrón MC, Yagüe S, Junca H, Carbajo JM, Zapico E, Silva R, Arana-Cuenca A, Tellez A, González AE. 2008. Research in Microbiology. 159(2):103-119. • Molecular detection and diversity of novel diterpenoid dioxygenase ditA1 genes from proteobacterial strains and soil samples. Witzig R, Aly HAH, Strömpl C, Wray V, Junca H and Pieper DH. 2007. Environmental Microbiology. 9 (5): 1202-1218. • Eight Americas: a new definition for "Americas"? Junca H. 2007. (Correspondence) PLoS Medicine. Jan;4(1):e42. • Toluene/biphenyl dioxygenase gene diversity assessed by culture dependent and culture independent approaches in benzene polluted soils: links between benzene biodegradation and isopropylbenzene dioxygenase-like genes.Witzig R, Junca H, Hecht HJ and Pieper DH. 2006. Applied & Environmental Microbiology. 72(5), 3504-3514. • Alternative primer sets for PCR detection of genotypes involved in bacterial aerobic BTEX degradation: Distribution of the genes in BTEX degrading isolates and in subsurface soils of a BTEX contaminated industrial site. Hendrickx B, Junca H, Vosahlova J, Lindner A, Rüegg I, Bucheli-Witschel M, Faber F, Egli T, Mau M, Schlömann M, Brennerova M, Brenner V, Pieper DH, Top EM, Dejonghe W, Bastiaens L, and Springael D. 2005. Journal of Microbiological Methods. 64 (2), 250-265. • Difference in kinetic behavior of catechol 2,3-dioxygenase variants from a polluted environment. Junca H, Plumeier I, Hecht HJ, and Pieper DH. 2004. Microbiology-UK. 150 (12), 4181-4187. • Tannic acid interferes with the commonly used laccase-detection assay based on ABTS as the substrate. Terrón MC, López-Fernández M, Carbajo JM, Junca H, Téllez A, Yagüe S, Arana-Cuenca A, González T, and González AE. 2004. Biochimie. 86 (8), 519-522. • Identification and characterization of the alpha-L-arabinofuranosidase B of Fusarium oxysporum f. sp. dianthi. Chacón-Martínez CA, Anzola JM, Rojas A, Hernández JF, Junca H, Ocampo W, and Del Portillo P. 2004. Physiological and Molecular Plant Pathology, 64(4) 201-208 • Functional gene diversity analysis in BTEX contaminated soils by means of PCR-SSCP DNA fingerprinting: comparative diversity assessment against bacterial isolates and PCR-DNA clone libraries. Junca H and Pieper DH. 2004. Environmental Microbiology. 6 (2), 95-110 • Amplified functional DNA restriction analysis to determine catechol 2,3 dioxygenase gene diversity in soil bacteria. Junca H and Pieper DH. 2003. Journal of Microbiological Methods. 65, 697-708 • Identification of a new laccase gene and confirmation of genomic predictions by cDNA sequences of Trametes sp. I-62 laccase family. Gonzalez T, Terron MC, Zapico E, Yague S, Tellez A, Junca H, and

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Gonzalez AE, 2003. Mycological Research. 107, 727-735 • Tannic acid induces transcription of laccase gene cglcc1 in the white-rot fungus Coriolopsis gallica. Carbajo JM, Junca H, Terron MC, Gonzalez T, Yague S, Zapico E, and Gonzalez AE. 2002. Canadian Journal of Microbiology. 48, 1041-1047 Book chapters Junca H. 2016. Introduction to Primer-Based Detection of Microbial Genes, Particularly Those Encoding Enzymes for Aromatic/Aliphatic Hydrocarbon Biodegradation. Chapter · December 2015 DOI: 10.1007/8623_2016_201 Protocol Part of the series Springer Protocols Handbooks pp 1-7 Series ISSN: 1949-2448, Humana Press. Cubillos-Ruiz A, Junca H, Baena S, Venegas I, Zambrano MM. 2010. Beyond Metagenomics: Integration of complementary approaches for the study of microbial communities. In Metagenomics: Theory, Methods and Applications - Editor: Diana Marcos. Horizon Scientific Press. ISBN: 978-1-904455-54-7 Pérez-Pantoja D, Donoso R, Junca H, González B and Pieper DH. 2010. Phylogenomics of aerobic bacterial degradation of aromatics. In Handbook of Hydrocarbon and Lipid Microbiology. Editor-in-chief: Ken Timmis. Wiley-Blackwell. ISBN: 978-3- 540-77588-1 Junca H, Pieper DH. 2010. Functional marker gene assays for hydrocarbon degrading microbial communities - aerobic, In Handbook of Hydrocarbon and Lipid Microbiology. Editor-in-chief: Ken Timmis. Wiley-Blackwell. ISBN: 978-3-540-77588-1 Mehboob F, Saia F, Weelink S, Tan N, Junca H, Stams AJM and Schraa G. 2010. Microbial degradation of aliphatic and aromatic hydrocarbons with (per)chlorate as electron acceptor. In Handbook of Hydrocarbon and Lipid Microbiology. Editor-in-chief: Ken Timmis. Wiley-Blackwell. ISBN: 978-3-540-77588-1 Cubillos-Ruiz A, Junca H, Baena S, Venegas I, Zambrano MM. 2009. Beyond Metagenomics: Integration of complementary approaches for the study of microbial communities. In Metagenomics: Theory, Methods and Applications - Editor: Diana Marcos. Horizon Scientific Press. ISBN: 978-1-904455-54-7 El Fantroussi S, Agathos SN, Pieper DH, Witzig R, Cámara B, Gabriel-Jürgens L, Junca H, Zanaroli G, Fava F, Perez- Jimenez JR, Young LY, Hamonts K, Lookman R, Maesen M, Diels L, Dejonghe W, Dijk J & Springael D. 2006. Chapter IV. Biological Assessment and Remediation of Contaminated Sediments. In: Assessment and Remediation of ContaminatedSediments; Springer Verlag, pp 179-241. NATO Science Series: IV: Earth and Environmental Sciences #73: Assessment and Remediation of Contaminated Sediments. ISBN: 978-1-4020-4958-3. Editors: Reible D and Lanczos T, Bratislava. Junca H, Witzig R, Pieper DH. 2005. In situ detection of functional genes for aerobic aromatic degradation. In Tagunsghandbuch: Workshop Perspektiven molekularer und isotopischer Methoden zum Nachweis des natürlichen Schadstoffabbaus in Böden, BMBF-Förderschwerpunktes KORA und des DECHEMA-IAK "Umweltbiotechnologie Boden“ ISBN 3-89746-069-6. Frankfurt Main. Junca H, and Pieper DH. 2005. Chapter 12: Diagnosing the biodegradation potential of soils. In: Lens P, Grotenhuis T, Malina G, Tabak H (eds). Soil and Sediment Remediation. IWA Publishing. ISBN 1843391007. London.

Howard Junca, May 2016