hoying, james b - cardiovascular innovation institute (cii) · hoying, james b january, 2016 1...

Hoying, James B January, 2016

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VITAE

JAMES B. HOYING, Ph.D.

Current Positions: Chief, Division of Cardiovascular Therapeutics

Cardiovascular Innovation Institute

University of Louisville, Jewish Hospital/St. Mary’s Healthcare

302 E Muhammad Ali Blvd

Louisville, KY 40202

502-852-2170

[email protected]

Professor

Department of Physiology and Biophysics

University of Louisville

President and CEO

Angiomics, Inc.

Tucson, AZ

502-377-0112

[email protected]

EDUCATION

Post-Doctoral Fellow: Program of Excellence in Molecular Biology of the Heart and Lung,

NHLBI, NIH, 1998

University of Cincinnati, Cincinnati, OH

Department of Molecular Genetics

Major Field: Molecular Genetics, Genetic Mouse Models

Research: Molecular Mechanisms of Cardiovascular Disease and Cancer

Advisor: Tom Doetschman, PhD

Doctor of Philosophy: University of Arizona, Tucson, AZ, 05/1994

Physiological Sciences Graduate Program

Major area: Cardiovascular Physiology

Minor area: Cancer Biology

Dissertation: Cell-Matrix Interactions of Microvessel Endothelial Cells in

Response to Basic Fibroblast Growth Factor

Advisor: Stuart K. Williams, PhD

Master of Science Case Western Reserve University, Cleveland, OH, 1987

Department of Biology

Major area: Molecular and Cellular Biology

Minor area: Biotechnology

mailto:[email protected]


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Thesis: Cloning and Characterization of Rhizobium Meliloti Loci Required

for Symbiotic Root Nodule Invasion.

Advisor: Naomi Lang-Unnasch, PhD

Bachelor of Arts: Case Western Reserve University, Cleveland, OH, 1985

Department of Biology

Major area: Biology

Minor area: Biochemistry

Senior Project: Tobacco Meristem Culture Methods

PROFFESIONAL EXPERIENCE

20014-present Professor* Department of Physiology and Biophysics,


Louisville, KY

*primary appointment switched to

Physiology from Surgery.

2008-2014 Professor* Dept. of Surgery


Louisville, KY

2007-present Chief Division of Cardiovascular Therapeutics,

Cardiovascular Innovation Institute


Jewish Hospital/St. Mary’s Healthcare,

Louisville, KY

2007-2008 Associate Professor Department of Surgery, University of

Louisville, KY

2003-2008 Associate Professor Division of Biomedical Engineering

Arizona Research Laboratories

University of Arizona, Tucson, AZ.

2003-2008 Associate Professor Department of Physiology

University of Arizona, Tucson, AZ

2000-2003 Assistant Professor Department of Physiology


1998-2003 Assistant Professor Division of Biomedical Engineering

Arizona Research Laboratories


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1994-1998 New Investigator Program of Excellence in Molecular

Biology of the Heart and Lung, NHLBI,

NIH

Department of Molecular Genetics

University of Cincinnati, Cincinnati, OH

ENTREPRENEURIAL EXPERIENCE

2010-present Co-founder Riviera Medical Technologies LLC

Louisville, KY

Technologies for cell-based therapies.

2004-present Co-founder and President-CEO Angiomics, Inc.

Tucson, AZ

Solutions for improving vascular health.

PROFESSIONAL MEMBERSHIP

North American Vascular Biology Organization (NAVBO), 2007-present

American Physiological Society, 1992-present

The Microcirculatory Society, 1992-present

American Heart Association, ATVB Council, 1998-present

International Society for Applied Cardiovascular Biology, 1998-present

Member, Sarver Heart Center, University of Arizona, 2001-08

Member, Physiological Sciences Graduate Program, University of Arizona, 1999-07


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PROFESSIONAL ACTIVITIES/SERVICE

Visiting Scientist and B Project Leader, Payload Team CCM-STS-118, Space Shuttle Flight STS-116.

NASA, Kennedy Space Center, FL, 2007

External Review

NIH/NIBIB Special Emphasis Panel/Scientific Review Group ZEB1 OSR-D (M2) P41 meeting,

October 2016

NIH/NIBIB Special Emphasis Panel NIBIB QUANTUM: Technological Innovation to Solve a Major

Medical or Public Health Challenge (U01) June 2016

NIH/NIBIB Special Emphasis Panel/Scientific Review Group ZEB1 OSR-D (M2) P41 meeting,

February 2016

NIH/NIBIB, Site Visit Review Panel, P41 National Resource Center for Biomaterials and Biofabricated

Platforms, March 2015

NIH, Lung Injury, Repair, and Remodeling Study Section, Ad Hoc reviewer, 2012

NIH, Special Emphasis Panel/Scientific Review Group ZHL1 CSR-V M1, 2011, 2012

NIH, Special Emphasis Panel/Scientific Review Group ZRG1 F10A-S 20, 2010

NIH, Special Emphasis Panel/Scientific Review Group ZRG1 CVRS-F 52, 2009, 2010

NIH, Special Emphasis Panel/Scientific Review Group ZRG1 CVRS-B 58, 2009

NIH, Special Emphasis Panel/Scientific Review Group ZRG1 CVS-P-02, 2008

NIH, CDD Study Section, Ad Hoc reviewer, 2007

NIH, Special Emphasis Panel/Scientific Review Group ZAG1 ZIJ, P01 review, 2005, 06

NIH, Special Emphasis Panel, Advances in Tissue Engineering Reparative Medicine Study Section,

2003

NIH, Mentored Scientist Development Award Study Section, 2003

NIH, NCI, U54/Interdisciplinary Teams in Molecular Targets, Special Emphasis Panel, Angiogenesis,

2000Invited external reviewer, Faculty Promotion and Tenure Review, Department of

Biomedical Engineering, Tulane University, Charlottesville, 2014

VA-ORD, Special Emphasis Panel/Scientific Review Group 2011/05 RRDO 1, 2011

American Heart Association, Vascular Biology & Blood Pressure/Regulation Basic Science 3, 2011-

present, Co-chair.

American Heart Association, Vascular Wall Biology I study section, Great American Affiliate,

American Heart Association, 2008, 2009, Chair.

American Heart Association, Combined GRA & National Peer Review Group, Vascular Wall Biology

I, 2001-2009


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American Heart Association, Affiliate 2B (Vascular Wall Biology) Research Peer Review Group,

1999-2001

Israel Science Foundation, Individual Research Grant, Invited Reviewer. 2013

ZonMw, Netherlands Organisation for Health Research and Development, TOP Grant Program, Invited

Reviewer. Netherlands, 2011, 2012, 2016

Invited external reviewer, Faculty Promotion and Tenure Review, Department of Molecular & Cellular

Medicine, Texas A&M, 2011

Invited external reviewer, Faculty Promotion and Tenure Review, Department of Bioengineering,

University of Virginia, Charlottesville, 2014

Conference Organization/Leadership

Invited Member, Scientific Advisory Committee; International Conference on Biofabrication 2016,

Winston-Salem, NC October 2016.

Invited Member, International Scientific Program Committee, World Congress for Microcirculation,

Kyoto, Japan, (WCMic2015) 25-27 September, 2015.

Organizer and Chair, Microvascular Plasticity Symposium, World Congress for Microcirculation,

Kyoto, Japan, (WCMic2015) 25-27 September, 2015

Organizer and Chair, Regenerative Medicine Track, Global Biotechnology Conference, Boston, MA,

July 2015.

Organizing Committee, International Academy of Cardiovascular Sciences - America Section,

Cardiovascular Forum for Promoting Centers of Excellence and Young Investigators in

Louisville, Kentucky on August 15-17, 2013, 2012

Invited Session Moderator, SESSION 6 Translational Sciences, IFATS International Conference, 2011.

Organizer and Chair, American Physiological Society-Sponsored Symposium entitled Genomics of

Angiogenesis and the Microcirculation, Experimental Biology Meeting, San Diego, CA, 2003

Organizing Committee, Weinstein Cardiovascular Development Conference held in Tucson, AZ, 1999

Session Chair, Selected Featured Topics Session, American Physiology Society, FGF Physiology,

Experimental Biology meeting, San Francisco, CA, 2006

Session Chair, Selected Vasculogenesis/Angiogenesis Feature Topics Platform. Experimental Biology

meeting, Washington, DC, 2004

Outreach

Program Director and Mentor, Junior Achievement of Kentucky Scholars Program, Jefferson County

Public High Schools. 2012, 2013, 2016.


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Invited guest instructor, Westport Middle School, Dana Hoben AP Biology Classes. Louisville KY,

2013.

Invited guest panelist, The Heart Facts, Kentucky Health 360 television series, Kentucky Educational

Television (KET) Network, 2012

Mentor, Speed Mentoring Sessions, 2011 Youth Science Summit, Louisville Science Center,

Louisville, KY, 2011

Invited Speaker, AP Biology classes, BASIS School, 2004

Faculty Mentor for Summer Institute Medical Ignorance, High School and Science Teacher Research

Fellows, Marlys Witte, Director, 2002

Invited Speaker, Medical Studies group, Catalina High School, Tucson AZ, 2001-02

Participant, Southwest BioPartnering Expo, 2001-02

Participant, Med Camp, 2000-06

Invited speaker, Medical Issues class, Catalina High School, Tucson AZ, 2001

Invited speaker, AP Biology class, Sabino High School, Tucson AZ, 2001

Participant, Phoenix HS Career Day, Public Affairs/College of Medicine, 2001

Judge, Life Sciences/Social Behavior, Science Fair Competition K-12, Southern Arizona Regional

Science and Engineering Fair (SARSEF), Tucson AZ, 1999-06

Faculty Mentor, NSF Summer Intern Program for High School Science Teachers, Dr. Supapan

Seraphin, Director, Tucson AZ, 1999-00

Invited speaker, Cactus Civitan, Tucson AZ, 1999

Invited speaker, Saguaro Rotary Club, Tucson AZ, 1999

Correspondent, American Society of Cell Biologists (ASCB) Letter to Young People Program, 1995-97

HONORS, AWARDS, AND RECOGNITION

Honors Recognition, An exclusive license with Tissue Genesis, Inc. for Adipose Stromal Vascular

Fraction Cell Epicardial Patch, Celebration of Faculty Excellence, University of Louisville,

2012

University Scholar re-appointment, University of Louisville, Louisville, KY, 2012

Honors Recognition, An exclusive license with Tissue Genesis, Inc. for a Adipose Stromal Cells to

Support Transplanted Tissue Integration and Inosculation, Celebration of Faculty Excellence,

University of Louisville, 2011

Fellow of the American Heart Association, Basic Cardiovascular Sciences (BCVS) Council, 2011

University Scholar, University of Louisville, Louisville, KY, 2008


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Keynote Address Speaker, Workshop in: Blood Flow in the Microcirculation: Function, Regulation

and Adaptation, Mathematical Biosciences Institute, Ohio State University, 2007

Taylor & Francis prize for Outstanding Innovation in Computer Methods in Biomechanics &

Biomedical Engineering, 6th International Symposium on Computer Methods in Biomechanics

and Biomedical Engineering, Team Member, 2004

Harry Rudney Post-Doctoral Merit Award, Department of Molecular Genetics, University of

Cincinnati, 1998

Research Merit Award, Post-Doctoral Research Poster Competition, University of Cincinnati/Ohio

Physiological Society, 1997

Travel Fellowship and Section Co-chair, Young Scientists Pre-meeting at Asilomar, 17th International

Congress of Biochemistry and Molecular Biology, 1997

Travel Award, Vascular Biology Meeting, 1997

Innovative Instrumentation Award, Microcirculatory Society, 1995

Proctor and Gamble Professional Opportunity Award for Meritorious Research, American

Physiological Society, Cardiovascular Section, 1994

National Heart, Lung, and Blood Predoctoral Trainee, University of Arizona. 1992-94

Physiological Sciences Scholarship Award, University of Arizona, 1993-94

Sigma Xi Research Award, 1992

1st place winner in Staff Division, Physiological Society of Philadelphia Poster Competition, 1991


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UNIVERSITY COMMITTEE ASIGNMENTS

Chair, Institutional Biosafety Committee, University of Louisville, 2015-present

Member, Faculty Search Committee, Director of the Center for Preventative Medicine, 2015.

Chair, Faculty Search Committee, Cardiovascular Innovation Institute, 2008

Member, Cardiovascular Innovation Institute, Building Design/Programming Committee,

Cardiovascular Innovation Institute, 2008

Member, Institutional Biosafety Committee, University of Louisville, 2008-present

Member, Cardiovascular Sciences Steering Committee, College of Medicine, University of Arizona,

2005-06

Member, Bio5/COM Search Committee for Mouse Transgenesis Faculty Director, University of

Arizona, 2005

Member, MSE Biomaterials Faculty Search Committee, University of Arizona, 2005

Member, AHSC Continuing Medical Education committee, University of Arizona, 2004

Member of the Medical Research Building Design Committee, AHSC, University of Arizona, 2003

Member, Selection Committee for BIO5 Predoctoral Fellowships, University of Arizona, 2003-04

Member of the Mouse Users Group Steering Committee headed by Dr. Parker Antin (CBA), University

of Arizona, 2003-06

Search Committee, Bioinformatics Faculty Positions in the IBSB, University of Arizona, 2002

Chairman, Recruiting/Admissions Committee for the Biomedical Engineering IDP, University of

Arizona, 2001-07

GATC Steering Committee, Arizona Research Labs, University of Arizona, 2000-07

Chairman, Activities Committee, Physiological Sciences Graduate Program, University of Arizona,

2000-02

Chairman, Resources Committee for the Biomedical Engineering IDP, University of Arizona, 1999-01

Executive Committee Member, Biomedical Engineering Graduate Program, University of Arizona,

1999-07

Executive Committee Member, Physiological Sciences Graduate Program, University of Arizona,

1999-07

UNIVERSITY SERVICE

Director, Multi-photon Confocal Microscopy Core, Cardiovascular Innovation Institute/Kentucky

Spinal Cord Injury Research Center, University of Louisville, 2010-present

Grant Pre-Submission Review Panel, Department of Surgery. University of Louisville, 2011-2014


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Assessments of faculty activities, 5-year Periodic Career Review, University of Louisville School of

Medicine, 2012

Mentor, Faculty Mentoring Program, School of Medicine, University of Louisville, 2011-present

Director/Management, Good Laboratory Practice (GLP) and Good Tissue Practice (GTP) Facilities,

Cardiovascular Innovation Institute, 2010-present

Faculty Search for Rodent Veterinary Staff Member, RRC, University of Louisville, 2009

Co-organizer for the Quantitative Approaches to Cell Signaling Workshop sponsored by the Applied

Mathematics IGERT, University of Arizona, 2003

Co-organizer and Co-chair for the Bi-annual Angiogenesis Workshop sponsored by Biomedical

Engineering Program, University of Arizona, 2002

Presentation on BioNanotechnology, College of Engineering and Mines Faculty Meeting, Promotion of

Interdisciplinary Research, University of Arizona, 2001

Founder/Co-Director, Genomics Research Laboratory, University of Arizona, 2000-2007.

Grant Reviewer, Clinical Faculty Research Fellowship Program, Robert S. Flinn Foundation, Arizona,

2000

Grant Reviewer, Pilot Projects Program in the SWEHSC, University of Arizona, 2000-06

Grant Reviewer, Pilot Projects Program, Dept. of Ophthalmology, University of Arizona, 2000-present

Grant Reviewer, Clinical Faculty Research Fellowship Program, Robert S. Flinn Foundation,

University of Arizona, 2000-06

Organizer and Chair for the Bi-annual Angiogenesis Workshop sponsored by Biomedical Engineering

Program, University of Arizona, 2000

Essay grader for the University’s Upper-Division Writing-Proficiency Examination (UDWPE),

University of Arizona, 1999, 01

Member of the Transgenic Mouse Users group headed by Dr. Bob Erickson (Pediatrics), University of

Arizona, 1998-05

Organizer/Coordinator, Vascular Research Group, University of Arizona, 1998-07

Co-organizer and Co-chair for the Bi-annual Angiogenesis Workshop sponsored by Biomedical

Engineering in cooperation with the Cancer Center, U. of Arizona, 1998

PROFFESSIONAL COUNCILS/BOARDS

Member, Membership Committee, the Microcirculation Society, 2012-2015

Associate Editor, Frontiers in Vascular Physiology, 2010-present

Member, Editorial Board, the Microcirculation journal, 2010-present.

Member, Executive Research Steering Committee, Pacific Mountain/West Region, American Heart


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Association, 2005-07

Member, Desert Mountain Affiliate Research Committee, American Heart Association, 2002-05

MANUSCRIPT PEER REVIEW

─ Acta Biomaterialia

─ American Journal of Pathology

─ American Journal of Physiology

─ Applied Optics

─ Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB)

─ Biotechniques

─ Biotechnology Progress

─ Cell Transplantation

─ Circulation Research

─ Developmental Dynamics

─ Frontiers in Vascular Physiology

─ Journal of the American Medical Association

─ Journal of Applied Physiology

─ Journal of Vascular Research

─ Journal of Visualized Experiments (JOVE).

─ Microcirculation

─ Microvascular Research

─ Nature Medicine

─ Nature Protocols

─ Physiological Genomics

─ PLoS One

─ Proceedings of the National Academy of Sciences (PNAS)

─ Stem Cell Translational Medicine

─ Tissue Engineering


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TEACHING

Courses/Lecturing

Spring 2016

Lecturer, PHZB 611: Advanced Human Cardiovascular Physiology, 4 credits, 7 graduate

students

Lecturer, NSCI 600: Translational Neurosciences, 3 credits, 8 graduate students

Fall 2016

Discussion leader, PHZB 609: Integrated Systemic Physiology, 3 credits, 4 graduate students

Spring 2009

Course Coordinator/Instructor, BE 400: Special Topics: Anatomy and Physiology 3 credits, 25

undergraduate students

Spring 2007

Course Organizer, BME 597G: Laboratory Rotations, 1-3 credits, 2 graduate students

Course Coordinator and lecturer, BME 511: Physiology for Biomedical Engineers, 3 credits,

13 graduate students

Instructor, BME 599: Independent Study in Biomedical Engineering, 1 credit, 2 graduate

students

Section Organizer and Lecturer, PSIO 603: Systems Physiology, Cardiovascular Physiology

section, 6 credit, 15 graduate students

Instructor, PSIO 610: Research Methods in Physiology, 1 credit, 1 graduate student

Fall 2006

Course Organizer, BME 597: Laboratory Rotations, 1-3 credits, 4 graduate students

Course Coordinator and lecturer, BME 410/510: Biology for Biomedical Engineers, 3 credits,


Lecturer, PSIO 503: Cell Physiology, Physiological Genomics lectures, 5 credit, 20 graduate

students

Course Organizer and lecturer, BME 576, Microvascular Biology, 3 credits, 8 graduate

students.

Spring 2006



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Course Coordinator and lecturer, BME 511: Physiology for Biomedical Engineers, 3 credits,


Session Facilitator, PSIO 602: Readings in Systems Physiology, Cardiovascular Physiology


Fall 2005



14 graduate students, 13 undergraduates


students

Spring 2005


Course Coordinator and lecturer, BME 411/511: Physiology for Biomedical Engineers, 3

credits, 16 graduate students, 23 undergraduate students



Fall 2004





students

Spring 2004






Fall 2003





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students

Spring 2003

Facilitator, iPBL Biochemistry, 9 medical students (see attached letter from Dr. Louise

Canfield)






Fall 2002





students

Spring 2002

Facilitator, iPBL Biochemistry, 9 medical students.


Course Coordinator and lecturer, BME 411/511: Physiology for Engineers, 3 credits, 8

graduate students, 11 undergraduate students



Fall 2001

Lecturer, MATH 596a: IGERT Forum, 2 credits, 15 graduate students




Lecturer, SURG 596I: Molecular Cardiovascular Biology, Genetic Models and Angiogenesis

lectures, 3 credits, 10 graduate students


students


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Spring 2001

Facilitator, iPBL Biochemistry, 9 medical students






Fall 2000



14 graduate students, 10 undergraduate

Lecturer, SURG 596I: Molecular Cardiovascular Biology, Genetic Models and Angiogenesis

lectures, 3 credits, 8 graduate students


students

BME 597x: Research methods, 2 credit, 2 graduate students

BME 900: Research, 8 credits, 2 graduate students

Spring 2000

Facilitator, iPBL Biochemistry, 8 medical students



graduate students, 11 undergraduate



Fall 1999


Course Coordinator, BME 595a: Topics in Tissue Engineering, 1 credit, new permanent course



Spring 1999

Course Co-Coordinator and lecturer, BME 411/511: Physiology for Engineers, 3 credits, 13



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Fall 1998

Co-Coordinator and lecturer, BME 510: Biology for Biomedical Engineers, 3 credits, 12

graduate students

Fall 1997

Co-Originator, EHS 848: Introduction to Bioinformatics and Biocomputing, 3 credits, 8

graduate students, University of Cincinnati

Spring 96, 97

Lecturer, DB 852: Developmental Biology I, Molecular Basis of Gastrulation Lecture, 5 credits,

15 graduate students, University of Cincinnati

Course Development

BE 400 Special Topics: Anatomy and Physiology for Bioengineering: Developed course syllabus and

content.

BME 576, Microvascular Biology, Course Founder, Co-developed syllabus and materials.

PSIO 603, Systems Physiology, Initiated restructuring of the course and participated in the redesign of

course content.

BME 410/510, Biology for Biomedical Engineering, Assisted in the development of course syllabus

and reading materials

BME 411/511, Physiology for Biomedical Engineering, Assisted in the development of course syllabus

and reading materials

BME 595A, Developed course syllabus and reading lists

BME 597, Research Methods in Biomedical Engineering, Re-designed lab rotation format and

reporting

iPBL (integrated Problem-Based Learning) Biochemistry, Assisted in the redesign of the iPBL Program

for 1st year med students

Journal Clubs and Discussion Groups

Coordinator, CII Regenerative Medicine Journal Club, 2009-present

Faculty Advisor, Cardiovascular Engineering Journal Club, 2004-2007


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Coordinator, Vascular Research Group, Faculty, Fellows, Post-Docs, Graduate Students,

Undergraduate Students, 1998-2007

Transgenics Users Group, Faculty, Fellows, Post-Docs, Graduate Students, Undergraduate Students,

1998-2007

TRAINING/MENTORING

Faculty

Paula Chilton, PhD Research Asst. Professor, Department of Surgery, Christina M. Kleinert Research

Institute, University of Louisville, 2015-present

Marvin Morris, MD, Assistant Professor, Division of Vascular Surgery, Department of Surgery,

University of Louisville, 2010-2013

Chad Stiening, PhD, Research Translation and Advancement. Chief Science Officer, KyPha, Inc.,

Louisville, KY, 2009

Sabbatical Host for Dr. Srini Raghavan, PhD, Material Science and Engineering Dept., University of

Arizona, 2006

Post-Doctoral Fellows (degree institution, research area, current position)

Amanda LeBlanc, PhD, (West Virginia University), Coronary microcirculatory repair. Assistant

Professor, OB/GYN University of Louisville, Cardiovascular Innovation Institute, 2009-2012

Carlos Chang, PhD, (University of Arizona), Microvascular network formation and engineering. Staff

Scientist, St. Joseph Translational Research Institute, Atlanta GA, 2008-2011

Laxminaryanan Krishnan, PhD, (University of Utah), Prevascularized islet transplantation device.

Senior Research Fellow, Georgia Institute of Technology, Atlanta, GA, 2008-2012

Sara Vasconcelos, PhD, (University of Alabama Birmingham), Angiogenesis guidance and

inosculation. Assistant Scientist, University Health Network, University of Toronto, Toronto,

Cananda, 2008-2010

Chad Stiening, PhD, (University of Arizona), Post-angiogenesis genetics. Chief Science Officer,

KyPha, Inc., Louisville, KY 2007-2009

Gabriel Gruionu, PhD, (University of Arizona), Microvessel-polymer interactions. Research Fellow,

Steele Laboratory of Tumor Biology at the Massachusetts General Hospital/ Harvard Medical

School, 2005-2007

Kameha Kidd, PhD, (University of Arizona), Tissue revascularization. Health Science Policy Analyst,

NIH/NCRR, Bethesda, MD, 2003

Chris Sullivan, PhD, (University of Arizona), Microvascular Remodling. Associate Professor, A.T. Still


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University, Phoenix, AZ, 2002-2003

Residents/Fellows

John Maijub, MD, Research Fellow, Department of Surgery, Regenerative Cell Dosing for Vascular

Repair, 2011-2013

Tolga Turker, MD, Research Fellow, Christine M. Kleinert Institute for Hand and Microsurgery; the

University of Louisville School of Medicine; the Kleinert Kutz Hand Surgery Group, 2011-

2012

Babak Salimi, MD Chief Resident, Pediatric Intensive Care, Capillary leak syndrome in post-cardiac

surgery pediatric patients. Assistant Professor, Indiana University Pediatric Intensive Care,

2011-2012

Sitara Kommareddi, MD, Microvascular gene expression. Surgical Resident Research Fellow, General

Surgeon, Saguaro Surgical. Tucson, AZ, 2001

Graduate students

Mentored students (date degree awarded, degree program, research area, current position)

Katlin Stivers, current PhD, Microbiology & Immunology, University of Louisville, Louisville KY

Logan Curtis-Whitchurch, current PhD, Physiology, University of Louisville, Louisville KY

Venkat Ramakrishnan, PhD 2015, MD/PhD Program, University of Louisville, currently completing

MD degree at the UofL.

Carlos Chang, PhD 2008, Biomedical Engineering, Microvascular Fabrication, Staff Scientist, St.

Joseph Translational Research Institute, Atlanta GA

Trevor Cardinal PhD, 2007, Physiological Sciences, Post-ischemia vasoreactivity, Asst. Professor,

Dept. of Bioengineering, Cal Poly San Luis Ibispo

Kevin Greer, PhD 2006, Biomedical Engineering, Computational genomics, Computational Genomic

Scientist, BIO5 Institute, University of Arizona

Mark Schwartz, PhD 2005, Biomedical Engineering, Genetics of biomaterials compatibility, Staff

Scientist, High-Throughput Genomics Inc.

Gabriel Gruionu, PhD 2004, Biomedical Engineering, Vascular remodeling, Research Fellow, Steele

Laboratory of Tumor Biology at the Massachusetts General Hospital/ Harvard Medical School

Chris Sullivan, PhD 2002, Physiological Sciences, FGF2 in vascular repair, Associate Professor, Dept.

of Biological Sciences, A.T. Still University, Phoenix AZ


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Ebonie Armstrong, current MS, Physiology, University of Louisville, Louisville KY

Maurice Jabbour, MS 2007, Biomedical Engineering, Antibody array-based detector, Post-doc,

Microbiology & Immunology, University of Arizona

Greg Dion, MS 2005 Biomedical Engineering, Biological-based sensing, Medical Student

Adam Budoff, MS 2000, Biomedical Engineering, Vascular microarray, Manager - Microarray

Services at AltheaDx

Service on Dissertation/Thesis Committee

Kathryn Deveau, (Anat. Sci and Neurology, University of Louisville), PhD current

Kathryn Harman, (Anat. Sci and Neurology, University of Louisville), PhD current

Adeola Adeyemo, (Pharmacology, University of Cincinnati), PhD current

Sauyma Vanderwyst, (Biomedical Engineering, University of Arizona), PhD 2011

Carlo Bartoli, (MD/PhD Program, University of Louisville), PhD 2011

Renata Ramos (Biomedical Engineering, University of Arizona), PhD 2008

Sharon Rodriquez (Physiological Sciences, University of Arizona), PhD 2008

Laxminarayan Krishnan (Bioengineering, University of Utah), PhD 2008

Alton Hiscox (Physiological Sciences, University of Arizona), PhD 2007

Kristen O'Hallorhan (Biomedical Engineering), University of Arizona, PhD 2007

Heather Salazaar (Physiological Sciences, University of Arizona), PhD 2006

Sam Powis (Biomedical Engineering, University of Arizona), PhD 2005

Jingyu Guo (Physiological Sciences, University of Arizona), PhD 2005

Cindy Smith (Biomedical Engineering, University of Arizona), PhD 2005

Yi Yang, (Material Science and Engineering, University of Arizona), PhD 2004

Jon Alberding (Biomedical Engineering, University of Arizona), PhD 2004

Ben Shepherd (Physiological Sciences, University of Arizona), PhD 2004

Brooke McGuire (Biomedical Engineering, University of Arizona), PhD 2003

Kameha Kidd (Physiological Sciences, University of Arizona), PhD 2002

Rob Kellar (Physiological Sciences, University of Arizona), PhD 2001

Don DalPonte (Physiological Sciences, University of Arizona), PhD 2001

William Brands (Biomedical Engineering, University of Arizona), MS2007


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Matt McReynolds (Physiological Sciences, University of Arizona), MS 2006

Richard Cangelosi (Applied Mathematics, University of Arizona), MS 2005

Jamie Dibble (Biomedical Engineering, University of Arizona), MS 2005

Sarah Swaim, (Biomedical Engineering, University of Arizona), MS 2004

Undergraduate Research

Erik Schuschke, Summer Undergraduate Research Program in Cardiovascular Research for Under-

represented Minorities and Disadvantaged Students, University of Louisville, 2015

Ebonie Armstrong, Summer Undergraduate Research Program in Cardiovascular Research for

Under-represented Minorities and Disadvantaged Students, University of Louisville,

2014

Kadi Simmons, HHMI Summer Research Program, Georgetown College, 2014

Andrea Howard, HHMI Summer Research Program, Georgetown College, 2013

Kaitlin Shumate, Summer Research, University of Georgia, 2011

Evan Davidson, Summer Intern, St. Louis University 2008

Lindsay Oliva Stepp, Summer Intern, Indiana University 2008

Sarah Leung, Summer Intern, Arizona State University 2006, 2007

Greg Powell, Summer Intern, Catholic University 2006, 2007

Marwan Mustafa, Research Assistant, Molecular & Cell Biology, U of Arizona 2007

Alicia Newkirk, Research Assistant, Med Tech Program, University of Arizona 2002, 2004

Janelle Kennedy, Research Assistant, Molecular & Cell Biology, U of Arizona 2004

Keri Vartanian, Senior Honors Project, University of Arizona 2003

Niral Patel, Senior Honors Project Mentor, University of Arizona 2003

Jennifer Thompson, Research Assistant, Ecol & Evol Biology, U of Arizona 2002

Brian Constance, Research Project Advisor, Materials Sci and Eng, U of Arizona 2002

Joann Chang, Research Project Advisor, Mechanical Engineering, U of Arizona 2000-2001

Community School Teacher Mentoring

Jessica Minnich, Summer Institute Medical Ignorance, High School and Science Teacher Research

Fellow. 2002

Kris Yarter, Sabino High School AP Biology, High School Biology Teacher Summer Research


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Program (NSF). 1999, 2000

PRESENTATIONS

Invited Talks

Invited Webinar speaker, Engineering a Functional Microcirculation, International Society of Applied

Cardiovascular Biology, ISACB Webinar Series, 2016.

Invited speaker, Tissue prevascularization, Department of BioEngineering, University of Pittsburgh,

Pittsburgh, PA, January 2016.

Invited speaker, The microvasculature as a therapeutic target, Department of Physiology, University of

Arizona, Tucson, AZ Sept. 2015

Invited speaker, Immune cell dynamics in white adipose tissue, Department of Microbiology &

Immunology, University of Louisville, Louisville KY April 2015.

Invited speaker, Manipulating the microvasculature and its environment, Experimental Biology,

Boston MA April 2015.

Invited speaker, Regulation of Angiogenesis/Collateral Artery Remodeling, Joint Meeting of the

Microcirculatory Society (MCS) and the North American Vascular Biology Organization

(NAVBO). Cape Cod, MA, Oct. 2013.

Invited speaker, Regenerative Angiogenesis Strategies, Cardiovascular Forum for Promoting Centers of

Excellence and Young Investigators, Louisville, KY, August 15-17, 2013.

Invited speaker, Formation of microvascular networks’ (role of stromal interactions directing

endothelial growth), The International Union of Physiological Sciences (IUPS) Congress,

Birmingham, UK, July 2013

Invited speaker, Microvascular Repair, Department of Biology, Bellarmine University, Louisville, KY,

April 2012

Invited speaker, Tissue perfusion during injury: quality not quantity, Kentucky Spinal Cord Injury

Research Center (KSCIRC), Louisville, KY, Feb. 2012

Selected speaker, Quantification of interactions of adipose derived stromal cells and the

microvasculature, IFATS Annual Conference. Miami FL, Nov. 2011

Invited speaker, Post-angiogenesis events in tissue neovascularization. Department of Physiology and

Biophysics, University of Louisville, KY, Sept. 2011

Keynote speaker, Engineering the microcirculation, Microvascularization for Tissue Engineering

Applications Symposium. TERMIS EU-2011 Annual Meeting, June 2011

Invited speaker, Engineering the coronary circulation. European Society of Cardiology conference.

Stockholm Sweden, Aug. 2010

Invited speaker, Reconstructing the microcirculation. Biomedical Engineering Program, University of


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Arizona, Tucson AZ, Oct. 2009

Invited speaker, Reconstructing the microcirculation. Kentucky Spinal Cord Injury Research Center,

University of Louisville, KY, Mar. 2009

Invited speaker, Reconstructing the microcirculation. Texas A&M University, College Station, TX,

Feb. 2009.

Invited speaker, Microvascular Reconstruction: Discovery and Application. American Medical Writers

Association. 68th Annual National Conference. Louisville, KY, Oct. 2008

Invited speaker, Defining and reconstructing the microvasculature in revascularization. ANGIONET-

British Microcirculation Society Meeting, London, UK Feb. 2008

Invited speaker, Dynamics of Cell Interfaces BioInterface 2007, Surfaces in Biomaterials Conference.

San Mateo, CA, Oct. 2007

Invited speaker, Microvascular Remodeling. Meeting on Blood Flow in the Microcirculation: Function,

Regulation and Adaptation. Mathematical Bioscience Institute. Columbus, OH, Jan. 2007

Keynote speaker (open to the public), Tissue Engineering: A Vascular Perspective, Meeting on Blood

Flow in the Microcirculation: Function, Regulation and Adaptation. Mathematical Bioscience

Institute. Columbus, OH, Jan. 2007

Invited speaker, Biologically-based molecular detection devices, Bioengineering Platform Session,

Sixth Arizona Biosciences Leadership Symposium, Phoenix AZ, June 2006

Invited speaker: Genomic tools. Frontiers in Medicine Series, College of Medicine, University of

Arizona, Feb. 2006

Invited speaker, Microvessel maturation in the post-angiogenic microcirculation. International

Microcirculation Meeting, British Microcirculatory Society/The Microcirculatory Society, New

Hampshire, USA, Sep. 2005

Invited speaker: Endothelium. Frontiers in Medicine Series, College of Medicine, University of

Arizona, Apr. 2005

Invited speaker: Vessel Differentiation and Angiogenesis. Bi-annual Grover Conference on the

Pulmonary Circulation, Sep. 2004

Selected speaker: Genetic Programs in Angiogenesis. Symposium on Genomics of Angiogenesis and

Microcirculation, Experimental Biology meeting, San Diego, CA: Apr. 2003

Selected Poster presentation: Identification of Putative Vascular Genetic Circuits in Angiogenesis and

Vascularization. Program in Genomics Applications, National Heart, Lung, and Blood Institute

(NHLBI), National Institutes of Health, Bethesda, MD, July 2003

Invited speaker: Prevascularizing Tissue Constructs. Biomedical Engineering, University of Arizona,

Tucson, AZ, Apr. 2003

Invited speaker: Genetic Programs in Mammalian Vascularization. Vertebrate Development

Laboratory, National Institute of Childhood Health and Development (NICHD), National

Institutes of Health, Bethesda, MD, May 2003


22

Invited speaker: Understanding Biological Systems through Large-scale Gene Expression

Measurements, Sarver Heart Center, University of Arizona, Tucson, AZ, Nov. 2003

Invited speaker, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh,

PA, Nov. 2002

Invited speaker, Department of Physiology, University of Arizona, Tucson, AZ, Nov. 2002

Invited speaker, Department of Chemical and Environmental Engineering, University of Arizona,

Tucson, AZ, Nov. 2002

Invited speaker, “Mathematics and the Genome”, Mathematics Awareness Week, University of

Arizona, Tucson, AZ, April 2002

Invited speaker, Bioinformatics Symposium for Drug Development. University of Toledo, Toledo, OH,

Nov. 2001

Invited speaker, ASME Summer Bioengineering Conf. Snowbird, Utah, June 2001

Invited speaker, Department of Bioengineering, University of Utah, Salt Lake City UT, Feb. 2001

Invited speaker, Department of Pathology, Emory University, Atlanta, GA, March 2001

Invited speaker. Arizona Cancer Center, University of Arizona, Tucson, AZ, Nov. 1999

Selected poster presentation Gordon Research Conference on “Angiogenesis and Microcirculation”.,

August 1999

Invited speaker. Department of Pediatrics, University of Arizona, Tucson AZ, June 1999

Invited speaker. Annual AALAS meeting. Tucson AZ, June 1998

One of 4 New Investigators selected to represent the Cincinnati Program of Excellence at the annual

POE meeting held at NIH. Cardiovascular functions of FGF2, May 1998

Invited seminar speaker. Department of Physiology. Eastern Virginia Medical School. Norfolk, VA,

Jan. 1998

One of 2 New Investigators selected to represent the Cincinnati Program of Excellence at the annual

POE meeting held at MIT. Uncoupled stimulus-response in platelets lacking transforming

growth factor-β1, Nov. 1996

Selected speaker. Thrombosis section, American Heart Association Annual Meeting, Oct. 1996

Selected speaker. Bioengineering section, Annual FASEB meeting, April 1990

Public Media Presentations/Articles

Featured scientist, “Researchers to Watch”, Dawn Yankeelov, The Lane Report, 2015.

Invited Expert Panel Member, “Stem cell treatment: Revolutionizing medicine and society”, public

discussion event, National Stem Cell Foundation, 2015

Featured Segment, Fareed Zakaria’s GPS Moonshot series, 2014


23

Featured scientist, “3D bioprinter to reproduce human organs, change the face of healthcare: The inside

story”, Lindsey Gilpin and Jason Hiner, cover story, TechRepublic, 2014;

http://www.techrepublic.com/article/new-3d-bioprinter-to-reproduce-human-organs/

Invited guest panelist, The Heart Facts, Kentucky Health 360 television series, Kentucky Educational

Television (KET) Network, 2012

Feature article, Innovation in Cardiovascular Health. Courier-Journal Newspaper, Louisville, KY,

2009

Feature article, Positive Flow, Business First of Louisville, Louisville, KY, 2009

Abstracts (presented as either a poster or a talk)

Golway M and JB HOYING. Vascularized In vitro Perfusion Module (VIPM™) 3D Bio-printed with

the BioAssemblyBot™. Int. Soc. Applied Cardiovascular Biology (ISACB). 15th Biennial

meeting. Banff. 2016.

Candiello JE, Grandhi TSP, Dale J, Beare J, Rege K, Hoying JB HOYING, Kumta P, and Banerjee I.

Biomaterial Based Engineering of Vascularized Pancreatic Islet Organoids Derived from

Human Pluripotent Stem Cells (hPSCs). AICHe Conference 2016.

Stivers K, Dale J, Beare J, Chilton P, and JB HOYING. Tissue-resident myeloid-derived suppressor

cells in lean adipose. Research!Louisville 2015.

Edgar LT, JB HOYING, and Weiss JA. Predictions of Sprouting Angiogenesis within Heterogeneous

Extracellular Environments. BMES 2015.

Nevitt C, Grant, Katelyn, Jeff, Sarah, JB HOYING, and LeBlanc AJ. Physiological Levels of

Thrombospondin-1 Decrease NO-Dependent Vasodilation in Coronary Microvessels from Aged

Rats. Research!Louisville 2015.

Loganathan G, Ramakrishnan V, Boyd NL, Beare J, Tweed B, Kleinert L, Hughes MJ, JB HOYING,

Williams SK, Appakalai BN. Stimulation of intraislet endothelial cells to form peri-islet

capillaries (PIC’s) in human and rat islets: Role of adipose-derived stromal vascular fraction

(SVF) cells and ECGS solution. 2015 Joint Congress of the IPITA-IXA-CTS.

J.B. HOYING. Microvessel adaptation and plasticity in microvascular regeneration. GBC 2015.

Curtis-Whitchurch L, Dale J, Reed R, Doetschman T, and J.B. HOYING. Conditional deletion of the

planar cell polarity protein Vangl2 in vascular endothelial cells in mice. KY-APS 2015.

Ramakrishnan V.M., Yang JY, Tien KT, Maijub JG, Burchell PO, Bocard BR, Williams SK, Morris

ME, J.B. HOYING, Wade-Martins R, West FD, and Boyd NL. Generation of an Engineered,

Vascularized, iPSC-Derived Hepatic Module. TERMIS 2015.

Ramakrishnan V.M., Tien K.T., McKinley T.R., Bocard B.R., Williams S.K., J.B. HOYING, Boyd

N.L. Vascular Self-Assembly of Human Stromal Vascular Fraction Cells is Mediated by Wnt5a.

American Heart Association 2015.

http://www.techrepublic.com/article/new-3d-bioprinter-to-reproduce-human-organs/


24

Muradashvili N, Tyagi R, Dale JR, Benton RL, Tyagi SC, JB HOYING, and Lominadze D. Adipose

stromal vascular fraction cell treatment mitigates increased cerebrovascular permeability after

traumatic brain injury. National Neurotrama Society 2015.

Nevitt CD, Aird AL, JB HOYING, LeBlanc AJ. Age-related dysfunction in neovascularization

potential of adipose-derived stromal vascular fraction. AHA National Conference. 2014.

Muradashvili N, Tyagi R, Dale J, Benton RL, Tyagi SC, O’Toole TE, JB HOYING, Lominadze D.

Traumatic brain injury-induced increased pial venular permeability is ameliorated with

endothelial progenitor cells and the stromal vascular fraction cells in mice. AHA National

Conference. 2014.

Ramakrishnan VM, Burchell PO, Beier JI, Dale JR, Arteel GE, JB HOYING, Williams SK, and Boyd

NL. Adipose Stromal Vascular Fraction-Derived Vasculature Supports Implantation of

Parenchymal Hepatocytes. Research!Louisville 2013. Louisville, KY.

Touroo JS, JB HOYING, Williams SK. Macro and Micro Blood Vessel Mimics: In Vitro Models of

the Vascular System. Biointerface 2013. Minneapolis, MN.

Touroo JS, JB HOYING, Williams SK. Automated Encapsulation of Adipose Stromal Vascular

Fraction Cells in Alginate Hydrogel Spheroids Using a Direct-Write 3D Printing System.

IFATS 2013. New York, NY.

Hoying AM, Williams SK, and JB HOYING. Micro-physiological Assay for Angiogenesis. Kentucky

Innovation and Entrepeneurship Conference 2013. Lexington, KY.

JB HOYING. Post-Angiogenesis Network Architecture is not Pre-Determined in the Adult. Joint

Conference of the Microcirculatory Society and the British Microcirculatory Society. 2012.

Oxford, UK.

Ramakrishnan V.M., Maijub J.G., Morris M.E., J.B. HOYING, Williams S.K., and Boyd N.L.

Development of an engineered implantable vascularized cell-based LDL apheresis device.

Research!Louisville. 2012. Louisville, KY.

Krishnan L, JB HOYING, and Williams SK. Microvascular Angiogenesis and Maturation in the

Diabetic Microenvironment. Research!Louisville. 2011. Louisville, KY.

Kmetz D, Tinney JP, Dale J, Krishnan L, JB HOYING, and Keller BD. Characterization of Vascular

Maturation within Engineered Cardiovascular Tissues. Research!Louisville. 2011. Louisville,

KY.

Dale JR, Breite D, Clayton L, Dwulet F, McCarthy R, JB HOYING, and Williams SK. Impact of

Enzyme Composition on Adipose-derived Stromal Vascular Fraction Cell Isolation. IFATS

International Conference. 2011 Miami, Fl. (selected talk).

Krishnan L, Nunes SS, Chang CC, Williams SK, and JB HOYING. Stromal Vascular Fraction Cells

Potentiation Of Neovascularization Across Tissue Interfaces Is VEGF Dependent. IFATS

International Conference. 2011 Miami, Fl. (selected talk).

Krishnan L, Nunes SS, Williams SK, and JB HOYING. Quantification of Interactions of Adipose

Derived Stromal Cells and the Microvasculature. IFATS International Conference. 2011 Miami,

Fl. (selected talk).


25

LeBlanc AJ, JB HOYING, and Williams SK. Adipose Stromal Vascular Fraction Cells Preserve

Coronary Perfusion When Used Immediately After Ischemia. IFATS International Conference.

2011 Miami, Fl. (selected talk).

Boyd NL, Nunes SS, Krishnan L, Chang CC, Jokinen JD, and JB HOYING. Human embryonic stem

cell derived mesenchymal cells function as perivascular cells to stabilize endothelial networks

in three-dimensional collagen I constructs. Microcirculation Congress. 2010. Paris, France.

Nunes SS, Rekapally HR, Chang CC and JB HOYING. Vessel arterial/venous plasticity during adult

neovascularization. Microcirculation Congress. 2010. Paris, France.

Chang CC, Krishnan L, Reese S, Boland EB, Williams SK, and JB HOYING. In vitro patterned

microvessels lose alignment in vivo. Microcirculation Congress. 2010. Paris, France.

Leblanc AJ, HOYING JB, and SK Williams. Tissue-engineered cardiac patch stimulates angiogenesis

and improves coronary function after myocardial infarct. Microcirculation Congress. 2010.

Paris, France.

Boyd N, Nunes SS, Krishnan L, Chang CC, Jokinen JD, and JB HOYING. hESC derived

mesenchymal cells functionally stabilize HUVEC networks in 3D collagen I constructs.

Biomedical Engineering Society Annual Meeting. Austin TX. 2010.

Gruionu G, Galis Z, Secomb T, and JB HOYING. In Vivo Imaging of Microvascular Network

Development in a Tissue Engineered Construct. Biomedical Engineering Society Annual

Meeting. Austin TX. 2010.

Chang CC, Williams SK, Boland ED, and JB HOYING. Implanted Prepatterned Microvessel Patch

Develops Lumenized Networks within 1 week. Annual Hilton Head Workshop, "Regenerative

Medicine: Advancing to Next Generation Therapies" 2010. Hilton Head, SC. (selected talk).

Krishnan L, Chang CC, L Clayton, JB HOYING. Adipose derived stromal vascular fraction cells

potentiate neovascular sprout invasion of surrounding matrix. IFATS 2009. Daegu, S. Korea

(selected talk).

Krishnan L, U Utzinger, S Maas, S Reese, JA Weiss, S Williams, and JB HOYING. Extracellular

Matrix Stiffness Modulates Microvascular Morphology During Early Sprouting Angiogenesis

in Vitro. Proceedings of the ASME 2009 Summer Bioengineering Conference (SBC2009) June

17-21, Resort at Squaw Creek, Lake Tahoe, CA, USA. (selected talk).

Underwood C, L Krishnan, A Fung, S Reese, JB HOYING, and JA Weiss. Quantification of Temporal

Collagen and Microvessel Alignment Induced by Anchored Boundary Conditions During

Angiogenesis. Proceedings of the ASME 2009 Summer Bioengineering Conference (SBC2009)

June 17-21, Resort at Squaw Creek, Lake Tahoe, CA, USA. (selected talk).

Nunes SS, MA Maddie, RL Benton, JB HOYING. Angiogenic potential of isolated microvessel

fragments is independent of their tissue origin. FASEB J. 2009 23:592.9. (selected talk).

Krishnan L, CC Chang, SK Williams, and JB HOYING. Role of insulin and hyperglycemia in

angiogenic sprouting and microvessel survival. FASEB J. 2009 23:592.4.

Rekapally H, CM Stiening, and JB HOYING.Alignment-dependent regulation of vangl2, a planar cell

polarity core member, in adult endothelial cells. FASEB J. 2009 23:767.2.


26

Boyd NL, SS Nunes, JB HOYING, and SL Stice. α-Smooth Muscle Actin Differential Regulation in

Human Embryonic Stem Cell Derived Mesenchymal Cells. FASEB J. 2009 23:1030.2.

Chang CC and. In Vitro Perfused Microvessel Networks. Cardiovascular Pathology. 17(4):268.

September. 2008. Bordeaux, France. (selected talk).

Z Rivera, JB HOYING, and PB Hoyer. Residual ovarian tissue of follicle-depleted mice expresses

genes for androgen synthesis and metabolism of xenobiotic compounds. Society for the Study

of Reproduction. May. 2008. Kona, HI.

Z Rivera, JB HOYING, PB Hoyer. Enriched expression of lipid biosynthetic genes in follicle-depleted

mouse ovaries depleted mouse ovaries. FASEB J. March. 2008. 22:1188.10. San Francisco, CA.

CJ Underwood, L Krishnan, N Kirkpatrick, W Brands, U Utzinger, JE Guilkey, JA Weiss, JB HOYING.

Biomechanical Interactions of Angiogenic Microvessels with the Extracellular Matrix. Trans-

Institute Angiogenesis Research Program Bi-annual Meeting. November. 2007. Bethesda, MD.

L Krishnan, CJ Underwood, SA Maas, B Ellis, TC Kode, JB HOYING, JA Weiss. Microvascular

orientation during angiogenesis: effects of mechanical stretch and boundary conditions

Proceedings of the ASME 2007 Summer Bioengineering Conference. July. 2007. Orlando, FL.

TR Cardinal and JB HOYING. A modified fluorescent microsphere-based approach for determining

resting and hyperemic blood flows in individual murine skeletal muscles. Mathematical

Bioscience Institute Workshop. January. 2007. Columbus, OH.

TR Cardinal, CJ Sullivan, and JB HOYING. Targeted disruption of Fgf2 impairs blood flow control in

the posterior gracilis during revascularization. FASEB J. 20:LB9. 2006.

RP Rhoads, X Ling, TR Cardinal, S Sheehan, M Morales, R Chetham, JB HOYING, and R Allen.

Aging impairs the ability of satellite cells to induce angiogenesis in vitro. FASEB J. 20:LB34.

2006.

AM Hiscox, AL Stone, G Gruionu, EB Patula, JB HOYING, and SK Williams. Development of an

islet-stabilizing implant construct. FASEB J. 20:A299. 2006.

CM Stiening, MB Abdallah, JB HOYING, and RJ Collier. Effect of lactogenic stimuli and serotonin

on secretory activation of bovine mammary epithelial cells (BMEC). FASEB J. 20:A616. 2006.

G Gruionu, JB HOYING, and TW Secomb. Increased arterial wall volume following chronic

reduction of blood flow. FASEB J. 20:A710. 2006.

CC Chang and Hoying JB. Patterned 3-D growth of microvessels. FASEB J. 19:A158. 2005.

CA Smith, Warren WL, Hoying JB, and Williams SK. Utilizing a three-dimensional bioassembly tool

to fabricate spatially organized multicellular vascular constructs. FASEB J. 19:A160. 2005.

C Stiening, Hoying J, and Collier R. Profiling gene expression during mammary epithelial cell

differentiation. FASEB J. 19:A1004. 2005.

TR Cardinal, Sullivan CJ, and Hoying JB. A fluorescent microsphere-based method for assessing

regional blood flows in the mouse hindlimb. FASEB J. 19:A1270. 2005.

JE Guilkey JE, Hoying JB, and Weiss JA. Large-scale modeling of the mechanical behavior of


27

multicellular constructs. ASME Bioengineering Conference. 2005.

G Gruionu, Stone AL, Hiscox A, Williams SK, Hoying JB. The effects of pancreatic islets on a tissue

engineered microvascular network. BMES Conference. 2005.

S Powis, Hoying JB, Riley MR, and Williams SK. Acquired microbial resistance to chlorine dioxide.

Antimicrobial Resistance conference sponsored by the National Foundation for Infectious

Diseases. 2005. (selected talk).

SK Beck, Chen HYS, Greer KA, Schwartz MA, Hoying JB. Complex activities of Wnt/Frizzled

signaling in microvascular maturation. FASEB J. 19(4): A163, 2005.

TR Cardinal, Sullivan CJ, Hoying JB. A fluorescent microsphere-based method for assessing regional

blood flows in the mouse hindlimb. FASEB J. 19(5): A1270, 2005.

CM Stiening, Abdallah MB, Greer K, Hoying A, Hoying J, Collier R.J Profiling Gene Expression

During Mammary Epithelial Cell Differentiation. FASEB J. 19(5): A1005, 2005.

G Gruionu, JB HOYING, MH Laughlin, GM Constantinescu, LG Gruionu, and TW Secomb.

Simulation of blood flow and structural adaptation in arteriolar arcade network of miniature

swine triceps muscle: Maintenance of perfusion following partial removal of blood supply.

FASEB J. 18(5): A255, 2004.

MA Schwartz, AL Stone, KA Greer, SK Williams, and JB HOYING. Using gene expression profiles

to determine the role of laminin 5 modification in polymer associated healing. FASEB J. 18(5):

A759, 2004.

KR Kidd, KA Greer, JB HOYING, and BM Weinstein. Molecular analysis of arterial-venous

differentiation using oligonucleotide microarray technology in the zebrafish. FASEB J. 18(5):

A776, 2004.

MR Reynolds, KM Taylor-Barcia, KA Greer, JB HOYING, and HL Brooks. Analysis of inner

medullary collecting duct gene expression in AQP1KO mice. FASEB J. 18(5): A1021, 2004.

Y Zhang, JE.Guilkey, JB HOYING, and JA.Weiss. Mechanical simulation of multicellular structures

with the material point method. 6th International Symposium on Computer Methods in

Biomechanics and Biomedical Engineering.

CS Weber, JB HOYING and RM Lynch. Capacitative Ca2+ influx is enhanced in aortic endothelial

cells from sarco-(endo)plasmic reticulum Ca2+ATPase isoform 3 knockout mice. FASEB J.

17(4): A43, 2003.

HYS. Chen, CS Weber, RM Lynch, and JB HOYING Altered regulation of calcium handling proteins

in endothelial cells of the Sarco-(endo)plasmic Reticulum Ca2+ATPase Isoform 3 Knockout

Mouse. FASEB J. 17(4): A43, 2003.

AM Hoying, KA Greer, MA Schwartz, JB HOYING and SE Klewer. High Quality, High Density,

Low Cost Fabrication of cDNA and Oligo Glass Microarrays. FASEB J. 17(4): A77, 2003.

KA Greer and JB HOYING Implementation of a Statistical Linear Model for Microarray Data

Analysis. FASEB J. 17(4): A78, 2003.

CJ Sullivan, KA. Greer, JB HOYING differential gene expression in the ischemic hindlimb in the


28

absence of FGF2. FASEB J. 17(4): A78, 2003.

MA Schwartz, AL. Stone, KA. Greer, KR. Kidd, SK. Williams, JB. Hoying. Evidence for a Cyclical

Nature in Chronic Healing Response Associated with Artificial Device Implantation using Gene

Expression Analysis. FASEB J. 17(4): A81, 2003.

I. Jongewaard, KA Greer, A Person, K Pogreba-Brown, JB HOYING, P Antin, R Runyan, and S

Klewer. Genes profiling of the developing chicken cardiovascular system using DNA

microarray and novel computational algorithms. FASEB J. 17(4): A158, 2003.

MR. McReynolds, KA. Greer, AM. Hoying, MA. Schwartz, JB HOYING and HL Brooks. Effects of

Dehydration on Gene Expression: A cDNA Array Study in Mouse Renal Medulla. FASEB J.

17(4): A482, 2003.

KR. Kidd, HYS Chen, KA. Greer, MA Schwartz, JB HOYING. Tissue vascularization is associated

with distinct vascular gene expression patterns. FASEB J. 17(4): A500, 2003.

CM. Smith, HYS Chen, J Rodriquez, J Barton, and JB HOYING. Three dimensional analysis of mural

cell coverage in microvascular collagen gels. FASEB J. 17(4): A500, 2003.

BR Shepherd, JB HOYING, SK Williams. Implantation of a tissue engineered microvascular graft

reduces infarct size in a murine model of myocardial infarction. FASEB J. 17(4): A500, 2003.

KB. Vartanian, HY.S. Song, KR. Kidd, H Wang and JB HOYING. Acceleration of angiogenesis in

microvessel treated with thrombin TP508. FASEB J. 17(4): A501, 2003.

G Gruionu, TW Secomb, and JB HOYING. Spatial and temporal heterogeneity during structural

remodeling of the mouse gracilis artery. FASEB J. 17(4): A551, 2003.

JA Szivek, MM Fernandez, WA Grana, DS Margolis, F Blumenkron, PD Calvert, PP Bourque, R

Vaidyanathan, and JB HOYING. Preliminary tissue engineering studies of cartilage and bone

on PBT scaffolds. Annual meeting of the Surfaces in Biomaterials Foundation. BioInterfaces

1:20. 2002.

L Krishnan, JA Weiss, MD Wessman, JB HOYING, R Das. Viscoelastic characterization of collagen

gel constructs. 4th World Congress of Biomechanics, Calgary, CA, July 2002.

L Krishnan, H Song H, JB HOYING, R Das, JA Weiss. Angiogenesis alters the material properties of

the extracellular matrix. Submitted, 49th Annual meeting of the Orthopaedic Research Society,

July 2002.

R Das, L Krishnan, C Evensen, JB HOYING, JA Weiss. Alterations in angiogenesis- and ECM-related

gene expression following partial medial collateral ligament injury. Submitted, 49th Annual

meeting of the Orthopaedic Research Society, July 2002.

JB HOYING, Helen Y.S. Chen, Benjamin Shepherd, and Stuart K. Williams. Rapid perfusion of a

prevascularized tissue construct following implantation. Cardiovascular Pathology. 11(1):47.

2002.

Schwartz MA, A Stone, SK Williams, and JB HOYING. Angiogenesis-related gene expression during

healing associated with polymer implants. Cardiovascular Pathology. 11(1):59. 2002

CJ Sullivan and JB HOYING. FGF2 is not essential during flow-dependent arterial remodeling in the


29

mouse. Cardiovascular Pathology. 11(1):18. 2002.

G Gruionu, TW Secomb, and JB HOYING. Structural Remodeling of the Murine Gracilis Muscle

Vasculature Following Permanent Interruption of Blood Supply. Cardiovascular Pathology.

11(1):44. 2002.

Greer K and JB HOYING. A comparison of hierarchical clustering algorithms on simulated

microarray data. The Physiologist 45(1):67. 2002.

Schwartz MA and JB HOYING. Describing angiogenesis phenotypes at the gene expression level

using cDNA microarrays. The Physiologist 45(1):76. 2002.

CJ Temm-Grove, JB HOYING, and DJ Hartshorne. Pericyte protein expression when activated

through the Rho signaling pathway. Keystone in Angiogenesis. 2001.

H Wang, M Schwartz, J Convery, JB HOYING, and JT Ryaby. Tp508, a synthetic thrombin peptide,

activates angiogenesis-related genes during femoral fracture healing. Frontiers of Skeletal

Biology. 2001.

Weber, C.S., JB HOYING, and RM Lynch. Altered Ca+2 homeostasis in aortic endothelial cells from

Sarco(endo)plasmic reticulum Ca+2 ATPase isoform 3 (SERCA3) knockout mice. FASEBJ.

15(5):A1117. 2001.

Sullivan C.J. and JB HOYING. Flow-dependent outward and inward arterial remodeling in the mouse.

FASEB J. 15(5):A950. 2001.

Shepherd B.R., JB HOYING, and SK Williams. Blood perfusion through a prevascularized tissue

construct following transplantation. FASEB J. 15(4):A116. 2001.

Budoff A, Greer K, Constance B and JB HOYING. Transcription profiling of angiogenesis using

DNA microarrays. Cardiovasc. Pathol. 9(4):234. 2000.

Gunatilaka T and JB HOYING. Screening for genes uniquely expressed by angiogenic microvessels

using DNA microarrays. Cardiovasc. Pathol. 9(4):235. 2000.

Gruoinu G., Sullivan CJ and JB HOYING. Mapping revascularization of the murine ischemic

hindlimb. FASEB J. 14(4):A35. 2000.

Sullivan C.J. and JB HOYING. Endogenous fibroblast growth factor-2 (FGF2) is not required for

ischemia-induced angiogenesis. FASEB J. 14(4):A35. 2000.

Sutliff, R.L., JB HOYING, V.J. Kadambi, E.G. Kranias, and R.J. Paul. Functional and biochemical

evidence for modulation of endothelial cell function by phospholamban. FASEBJ.

12(5):A957.1998.

HOYING JB, M. Yin, and T. Doetschman. Faulty IIb/3 activation in platelets from transforming

growth factor1- deficient mice. FASEB J. 11(9):A1168. 1997.

HOYING JB, M. Zhou, T. Doetschman, and C. Haudenschild. Comparison of vascular injury/repair

between FGF2 wild type and knockout mice. Microcirculation. 4(1):186.1997.

HOYING JB, M. Yin, and T. Doetschman. Abnormal platelet function in TGF1-deficient mice.

Circulation 94(8):I-580.1996.


30

HOYING JB and S.K. Williams. Measurement of endothelial cell migration using a linear migration

assay. FASEB J. 9(4):A645.1995.

HOYING JB and S.K. Williams. Reduced adhesion of human microvascular endothelial cells to

collagen I in response to basic fibroblast growth factor is mediated byβ1 integrin. FASEB J.

8(4):A45.1994.

HOYING JB and S.K. Williams. Differential effects of growth factors on in vitro angiogenesis in 3-

dimensional culture. FASEB J. 8(5):A1044.1994.

HOYING JB and S.K. Williams. Growth of freshly isolated microvessel fragments cultured within

collagen I gels. FASEB J. 7(3):A126.1993.

Chen, S-C., JB HOYING, and S.K. Williams. Diffusion rates of solutes in extracellular matrix

measured by fluorescence recovery after photobleaching. Mol. Biol. Cell 3:228a.1992.

HOYING JB and S.K. Williams. Differential responses of endothelial cells to tumor necrosis factor-

alpha. FASEB J. 3(4):A526.1991.

HOYING JB and S.K. Williams. Examination of endothelial cell monolayer permeability by total

internal reflection fluorescence microscopy. FASEB J 4(3):A415.1990.

HOYING JB, T.S. Schneider and S.K. Williams. Evaluation of gold labeled glucosylated albumin

transport across endothelial cell monolayers using rapid freeze fixation. J. Cell Biology.

109(4):203a.1989.

PATENTS AND DISCLOSURES

Issued Patents

US # 8,727,965, Methods and Compositions to Support Tissue Integration and Inosculation of

Transplanted Tissue and Transplanted Engineered Penile Tissue with Adipose Stromal Cells.

12/2015

US #12/562,832, Bioreactors, systems, and methods for vascularizing tissue constructs. 8/2015

US #7,862,652, Directional self-assembly of biological electrical interconnects. 1/2011

US #7,052,829, Prevascularized constructs for implantation to provide blood perfusion. 5/2006

US #7,029,838, Prevascularized constructs for implantation to provide blood perfusion. 4/2006

Pending/Provisional Patent Applications

US #61/818,294 Compositions and methods for treatment of vessel disease

US #14/426,618 Cell systems and methods for delivering disease-specific therapies

US #61/370,714 Prevascularized materials and methods of using the same

http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=5&f=G&l=50&co1=AND&d=PTXT&s1=Hoying.INNM.&OS=IN/Hoying&RS=IN/Hoying





31

US #61/449,390 Encapsulation and delivery systems for biological suspensions and methods of

making the same

US #20100196433 Prevascularized devices and related methods.

US #20100075293 Bioreactors, systems, and methods for vascularizing tissue constructs.

US #20090017553 Immunoassay-based microsensing using optical sensors

GRANTS AND CONTRACTS

Active

R01 HL131856-A1 (PI) 1/1/17 to 12/31/22

NIH/HLBI $1,250,000

Neovessel guidance in angiogenesis 20% effort

The major goal of this project is to define the interplay between biomechanical forces and angiogenic

factor signaling in guiding neovessel growth during angiogenesis. Recently scored 5%

R01 (Co-I; LeBlanc PI) 4/1/17 to 3/31/22

NIH/HLBI $1,886,659

Mechanisms of Cell Therapy to Improve Coronary Microvascular 10% effort

Dysfunction in Aging

The major goal of this project is to explore the dynamics of a cell-based therapy for myocardial

ischemia in the context of aging. Recently scored 4%

JHFE (Co-PI; LeBlanc, PI) 9/1/16 – 8/31/18

Microvascular Dysfunction in Women’s Hearts: A Novel Diagnosis $300,000

and Treatment Regime The major goal of this project is to develop a new diagnostic and cell-based therapy for microvascular

angina

MR120023P2 (Co-PI) 09/30/13 – 10/01/17

Department of Defense, Restorative Transplantation $1,777,421

Research Program 8% effort

Positioning Vascularized Composite Allotransplantation within the Spectrum of Transplantation

The goal of this project is to assess vasculopathies in vascularized tissue/organ transplants.

AFIRMII-CTA-09 (Co-PI) 07/01/2013 – 06/30/2018

US Army Medical Research and Materiel Command $824,354

Tolerization of Vascularized Composite Allografts 8% effort

The goal of this project is to assess adipose regenerative cell therapies in vascularized tissue/organ

transplant patients.

R21 (Co-I; Boyd PI) 4/1/16 to 3/31/18

NIH/HLBI $275,000


32

Patient Specific Hepato-Vascular Unit for LDL Clearance 5% effort

The major goal of this project is to develop a biologically-based apheresis system to treat familial

hypercholesterolemia.

P30 GM103507 (Core Director) 4/1/11 - 7/31/16

NIH/NCRR $1,123,125

Mechanisms of plasticity and repair after SCI 10% effort

This support expands the Core facilities and pilot grants program to the existing COBRE

Helmsley Charitable Trust Foundation (Core Director & project Co-I; Harkema PI) 1/1/16 – 12/31/19

Kentucky Spinal Cord Research Center $15,000,000

The Helmsley Center for Restorative Medicine: An Interdisciplinary, 5% effort

Collaborative Program in Medical Research for Spinal Cord Injury

This supports the Microscopy and Functional Imaging Core of KSCIRC/CII and vascular assessment of

spinal cord injury in a rat model.

Pending

1T32HL134644-01A1 (Mentor) 7/1/2017 to 6/30/2022

NIH/NIGMS $1,486,242

Current Trends in Stem Cell Therapies

The intent of this program is to provide focused post-doctoral training in the biology and application of

cell-based therapies.

1T32HL134627-01 (Mentor) 09/01/2016 to 08/31/2021

Title: Cardiovascular Sciences $1,546,492

The intent of this program is to provide focused pre-doctoral training in cardiovascular health and

disease.

1R25GM123933-01 (Mentor) 07/01/17 to 06/30/22

NIH $1,486,242

Bridge to Undergraduate Success (BUS)

The intent of this program is to provide biomedical research exposure to under-served undergraduate

students.

1T32 (Mentor) 09/01/16 to 08/31/21

NIH $945,636

Inflammation and Pathogenesis

The intent of this program is to provide focused pre-doctoral training in tissue injury and inflammation.

AHA Pre-doctoral Fellowship Curtis-Whitchurch (Sponsor) 7/1/2016 to 6/30/2020

Vangl2 as an organizer of membrane lipid rafts in endothelial cell signaling


33

R21 (Co-I; LeBlanc PI) 9/1/16 to 8/31/18

NIH/ASG $275,000

Reversing microvascular dysfunction in aging hearts 10% effort

The major goal of this project is to develop an intravenous, cell-based therapy to reverse coronary

microvascular dysfunction in aging, female hearts. in review.

R01 (Co-l; Boyd PI) 7/1/16 to 6/30/21

NIH/NIDDK $2,384,892

Patient Specific Hepato-Vascular Unit for LDL Clearance 5% effort

The major goal of this project is to develop a biologically-based apheresis system to treat familial

hypercholesterolemia. Revision in review

Research Accelerator Award (PI – sub; Banerjee U Pitt PI) 1/1/17 to 12/31/21

American Diabetes Association 3% effort

Engineering islet organoids with islet-specific vasculatures $200,000 (sub)

The major goal of this project is to incorporate a vasculature into iPSC-derived islet cell organoids that

support islet-like function.


34

Completed

VV Cooke Foundation 12/01/15 – 11/30/16

Pilot Program $10,000

Studies in Women’s Heart Health 2% effort

The goal is to perform pilot studies in support of a pre-clinical investigation of cell therapy for

microvascular angina.

Kosair Charities (PI) 11/01/12 – 12/31/15

Christine Kleinert Institute $280,000

Basic Science Studies to increase safety of transplantation 5% effort

The goal of this project is to investigate the ability of adipose regenerative cells to modulate immune

responses to vascularized tissue/organ transplant.

R01 DK078175 (Co-PI) 12/1/2007 to 11/30/2012-NCE

NIH/NIDDK $1,000,500

A Prevascularized Islet Immune-isolation Device 10% Effort

The goal of this project is to develop a prevascularized implantable device to support islet viability and

function.

R01 HL077683 (PI subcontract) 7/1/08 to 6/30/12 - NCE

NIH/NIBIB $540,000

Angiogenesis and the Extracellular Matrix 8% effort

The major goal of this project is to define the microscale biomechanical interactions of angiogenic

microvessels with the extracellular matrix (ECM) in order to develop a computational model of

angiogenesis.

R01EB007556-01 (PI) 7/1/07 to 6/30/11

NIH/NIBIB $3,000,000

Fabrication of Microvascular Tissue Fabrics 15% Effort

The goal of this project is to generate pre-patterned, two and three dimensional microvascular networks

capable of functioning as a blood perfusion system.

R01 HL099014 (Collaborator) 2/1/10 – 1/31/14

NIH/NHLBI $372,000 annual

Role of Glutathione S-Transferase P in Heart Failure 5% effort

The primary goal of this project is to define the mechanisms of GSTP-mediated heart failure.

[This grant transferred with Dr. Prabhu when he relocated to another University]

R41HL115849-01A1 (PI, Angiomics Inc.) 4/01/13 – 12/31/14

NIH/NHLBI $100,000

human Microvessel Culture System (hMCS) 10% effort

This project entails the development of a 3-D human-based in vitro angiogenesis assay for therapeutics

screening.


35

#COMMFUND-1400-RFP-014 (PI) 2/1/13 – 1/31/14

Kentucky Science and Engineering Foundation (KSEF) $75,000

Adipose Regenerative Cell Isolation System

This project is to develop an isolation kit of adipose regenerative cells for commercialization.

(PI subaward)

(PI) 10/1/11 – 9/30/13

Kosair Pilot Grant Program $50,000

Role of Vangl2 in Vascular Formation 5% effort

This is a pilot project developing exploratory data, including a conditional targeted knockout mouse of

Vangl2.

IRIG Pilot Grant Program (PI) 1/1/13 – 12/31/14

U of L VPR Office $5,000

Epigenetic Regulation of Adult Neovascularization and Vascular Stability

Generate pilot data in the investigation of epigenetic control of vascular maturation.

(PI subcontract) 7/1/10 - 10/30/14 (NCE)

James S. McDonnell Foundation $168,000

Architecture-dependent Signaling in Multicellular Networks 5% effort

The goal is to perform a combined experimental and theoretical study of calcium signaling in networks

of endothelial cells as prototypical models of complex architectures.

(Collaborator) 6/1/10 - 5/31/11 U. of Louisville Research Foundation $15,000

Role of estriol in placental vascularization during preeclampsia 2% effort

This project will evaluate effects of estriol on placental vascularization using both human tissue and a

mouse model of preeclampsia.

OICN081473 (Collaborator) 8/1/08 to 7/31/09

Abiomed, Inc. $561,060

Abiomed VAD Benchmark Trial 10% effort

The major goal of this project is to develop a panel of performance benchmarks and standards for the

clinical implementation of Abiomed ventricular assist devices.

0755710Z (PI) 7/1/07 to 6/30/10

AHA-Pacific Mountain Affiliate $66,000 annual

Wnt/Planar Cell Polarity Signaling in Post-angiogenesis

Microvascular Maturation

The major goal of this project is to define the role planar cell polarity plays in the maturation of new

vessels formed following angiogenesis.

06-027, (PI) 7/1/06 to 6/30/09

Arizona BioResearch Commission $50,000 annual


36

Gene Markers of Angiogenesis and the Microcirculation

The major goal of this project is to determine gene expression programs in vascular cells associated

with specific events in vascularization in mouse and human.

R01 HL077683, (PI subcontract) 1/1/04 to 12/31/08

NIH/NIBIB $165,000 annual

Angiogenesis and the Extracellular Matrix

The major goal of this project was to develop advanced modeling and imaging methods to determine

the effects of biomechanical conditioning on the angiogenesis process.

ECS 0303863, (Co-PI) 8/1/03 to 7/31/07

NSF/NIRT $250,000 annual

Reversible and Directed Self-Assembly of Bio-Molecular Templates for Nanotechnology Interconnects

The major goal of this project was to fabricate nano-scale, multi-dimensional, conductive, interconnects

using microtubules.

Juvenile Diabetes Research Foundation, (PI) 10/1/05 to 9/30/06

Microvascular Maturation in Diabetes $100,000

The major goal of the project was to characterize microvascular maturation in the normal and

hyperglycemic environment in the absence and presence of pancreatic islets

K02 HL67067, (PI) 8/1/01 to 7/31/06


SERCA3 in Vascular Physiology

The major goal of this award is to develop Dr. Hoying’s research career in vascular biology. Includes

support for expanded research activities beyond currently funded projects.

R01 HL63732, (PI) 2/1/00 to 1/31/05


Specificity in endothelial cell calcium signaling

The major goal of this project was to determine the role of SERCA3-managed calcium in endothelial

cell biology.

R01 HL63873, (Co-I) 11/1/00 to 7/31/05


Role of Laminin-5 in Implant Associated Angiogenesis

The major goal of this project was to examine the angiogenesis and neovascularization of polymers

modified with the major extracellular matrix proteins involved in microvascular homeostasis as

compared to laminin 5.

Role: Co-Investigator

R01 EB000660, (Collaborator) 9/30/02 to 9/29/06

NIH/NIBIB $182,000 annual

Sensate Scaffold for Orthopedic Tissue Repair


37

The major goal of this project is to develop an implantable sensate scaffolds in vivo for monitoring of

bone loading.


UofA Children Research Center (Co-PI) 7/1/04 to 6/30/05

Treatment of Type 1 Diabetes through Beta-Cell Replacement Therapy $100,000

The goal of this project is to test the hypothesis that the combination of a stable, pre-formed

microvasculature and vascular-compatible, immuno-isolation devices will lead to successful

implantation of ß-cell constructs.

Orthologic, Inc. (PI) 7/25/04 to 5/31/06

Angiogenic Potential of the Thrombin non-PAR analog, TP-508 $65,000 total

The goal of this project was to evaluate the angiogenic potential of the non-PAR agonist TP508 in vivo.

The Aircast Foundation, Inc., Collaborator: (Co-I) 12/00 to12/02

Effect of Anti-angiogenic Treatment on Ligament Healing $200,000

The goal of this project was to investigate the role angiogenesis plays in ligament healing emphasizing

the effects on ligament biomechanics. This project provided preliminary data for the currently active

R01 on angiogenesis and biomechanics.

HRSA, Equipment Grant, (Core Director) 9/02-8/03

Microarray Core Facility $984,488

An equipment grant to provide a proteomics-based discovery tool for the microarray core facility.

BES-9978820, (Consultant) 8/99 to 7/02

NSF $101,923

Vascular Characterization with OCT Techniques

The goal of this project was to examine a number of different vascular settings (microvascular and

macrovascular) with a new endoscopic optical coherence tomography system.


NIH/HLBI, T32 HL07955 (Co-I) 7/1/05 to 6/30/10

Graduate Training in Cardiovascular Biomedical Engineering $292, 000 annual

Role: Mentor/Steering Committee

NIH/NIBIB, T32 EB000809, Mentor 5/1/03 to 4/30/08

Graduate Training in Biomedical Imaging and Spectroscopy $302,640 annual

Role: Mentor

NIH/HLBI, T32 HL07249, Mentor/Steering Committee 6/1/02 to 5/31/07

Training in Cardiovascular Systems Physiology $143,750 annual

Role: Mentor

NIH/NIGMS, T32 GM08400-15, Mentor 7/1/02 to 6/30/07


38

Graduate Training in Systems and Integrative Physiology $187, 680 annual

Role: Mentor

PUBLICATIONS:

Published Articles- Peer Reviewed

1. Nevitt C, McKenzie G, Christian K, Austin J, Hencke S, Hoying J, and LeBlanc A. Physiological

levels of thrombospondin-1 decrease NO-dependent vasodilation in coronary microvessels from

aged rats. Am J Physiol Heart Circ Physiol 310: H1842-1850, 2016.

2. Sun J, Hoying JB, Deymier PA, Zhang DD, and Wong PK. Cellular Architecture Regulates

Collective Calcium Signaling and Cell Contractility. PLoS Comput Biol 12: e1004955, 2016.

3. Kaufman CL, Marvin MR, Chilton PM, Hoying JB, Williams SK, Tien H, Ozyurekoglu T, Ouseph

R. Immunobiology in VCA. Transplant international: official journal of the European Society for

Organ Transplantation. 2016

4. Ramakrishnan VM, Tien KT, McKinley TR, Bocard BR, McCurry TM, Williams SK, Hoying JB,

Boyd NL. Wnt5a regulates the assembly of human adipose derived stromal vascular fraction-

derived microvasculatures. PLoS One. 2016;11:e0151402

5. LeBlanc AJ, Hoying JB. Adaptation of the Coronary Microcirculation in Aging. Microcirculation

(New York, NY : 1994). 2016;23(2):157-67.

6. Xu J, Zheng S, Kralik PM, Krishnan L, Huang H, Hoying JB, Cai L, Carlson EC, Tan Y, Epstein

PN. Diabetes Induced Changes in Podocyte Morphology and Gene Expression Evaluated Using

GFP Transgenic Podocytes. International journal of biological sciences. 2016;12(2):210-8.

7. Deymier PA, Swinteck N, Runge K, Deymier-Black A, Hoying JB. Effect of sound on gap-

junction-based intercellular signaling: Calcium waves under acoustic irradiation. Phys Rev E Stat

Nonlin Soft Matter Phys. 2015;92:052711

8. Gabbur P, Hoying J and Barnard K. Multimodal probabilistic generative models for time-course

gene expression data and Gene Ontology (GO) tags. Math Biosci. 2015 268:80-91

9. Maijub JG, Boyd NL, Dale JR, Hoying JB, Morris ME, and Williams SK. Concentration Dependent

Vascularization of Adipose Stromal Vascular Fraction Cells. Cell Transplant. 2015 24:2029-39.

10. Ramakrishnan VM, Yang JY, Tien KT, McKinley TR, Bocard BR, Maijub JG, Burchell PO,

Williams SK, Morris ME, Hoying JB, Wade-Martins R, West FD and Boyd NL. Restoration of

Physiologically Responsive Low-Density Lipoprotein Receptor-Mediated Endocytosis in

Genetically Deficient Induced Pluripotent Stem Cells. Scientific Reports. 2015 5:13231.

11. Edgar LT, Hoying JB and Weiss JA. In Silico Investigation of Angiogenesis with Growth and

Stress Generation Coupled to Local Extracellular Matrix Density. Ann Biomed Eng. 2015 43:1531-

42.

12. Utzinger U, Baggett B, Weiss JA, Hoying JB. Gigavoxel Time-lapse Microscopy of Angiogenic


39

Sprouting. Angiogenesis. 2015 18(3):219-32 (selected for cover image).

13. Morris ME, Beare JE, Reed RM, Dale JR, LeBlanc AJ, Kaufman CL, Zheng H, Ng CK, Williams

SK, and Hoying JB. Systemically-delivered adipose stromal vascular fraction cells disseminate to

peripheral artery walls and reduce vasomotor tone through a CD11b+-cell-dependent mechanism.

Stem Cells Trans Med. 2015 Stem Cell Trans Med. 2014-0252.

14. Aird AL, Nevitt CD, Christian K, Williams SK, Hoying JB, and LeBlanc AJ. Adipose-derived

stromal vascular fraction cells isolated from old animals exhibit reduced capacity to support the

formation of microvascular networks. Exp Gerontol. 2015 Jan 21;63C:18-26

15. Hoying JB, Utzinger U, and Weiss JA. Formation of microvascular networks: role of stromal

interactions directing endothelial growth. Microcirculation. 2014 (4):278-89 invited review.

16. Underwood CJ, Edgar LT, Hoying JB, and Weiss JA. Cell-generated Traction Forces and the

Resulting Matrix Deformation modulate Microvascular Alignment and Growth during

Angiogenesis. Am J Physiol Heart Circ Physiol. 2014, 307(2):H152-64. Editor’s Pick.

17. Edgar LT, Hoying JB, Utzinger U, Underwood CJ, Krishnan L, Baggett BK, Maas SA, Guilkey JE,

and Weiss JA. Mechanical Interaction of Angiogenic Microvessels with the Extracellular Matrix. J

Biomech Eng. 2014 (2):021001. invited review.

18. Edgar LT, Underwood CJ, Guilkey JE, Hoying JB, and Weiss JA. Extracellular Matrix Density

Regulates the Rate of Neovessel Growth and Branching in Sprouting Angiogenesis. PLoS One

2014 9(1):e85178.

19. Krishnan L, Touroo J, Reed R, Boland E, Hoying JB, Williams SK. Vascularization and cellular

isolation potential of a novel electrospun cell delivery vehicle. J Biomed Mater Res A. 2014

102(7):2208-19

20. Long J, Deymier PA, Runge K, Hoying JB. Regulation of the frequency and wavelength of calcium

waves propagating in networks of interconnected cells: A simulation study. Trends in Cell &

Molecular Biology. 2014;9:19-33.

21. Krishnan L, Chang CC, Nunes SS, Williams SK, Weiss JA, and Hoying JB. Manipulating the

Microvasculature and its Microenvironment. Crit Rev Biomed Eng. 2013; 41(2):91-123. invited

review.

22. Williams SK, Touroo JS, Church KH, Hoying JB. Encapsulation of adipose stromal vascular

fraction cells in alginate hydrogel spheroids using a direct-write three-dimensional printing system.

Biores Open Access. 2013; 2(6):448-54.

23. Türker T, Murphy E, Kaufman CL, Kutz JE, Meister EA, Hoying JB. Response of dupuytren

fibroblasts to different oxygen environments. J Hand Surg Am. 2013; 38(12):2365-9.

24. LeBlanc A, Nguyen QT, Touroo JS, Aird AL, Chang RC, Ng CK, Hoying JB, and Williams SK.

Adipose-derived cell construct stabilizes heart function and increases microvascular perfusion in an

established infarct. Stem Cells Trans Med. 2013; 2(11):896-905.

25. Nunes SS, Maijub JG, Krishnan L, Ramakrishnan VM, Clayton LR, Williams SK, Boyd NL, and

Hoying JB. Generation of a functional liver tissue mimic using adipose stromal vascular fraction

cell-derived vasculatures. Nature: Scientific Reports. 2013; 3:2141. PMCID: PMC3701895.


40

26. Junkin M, Lu Y, Long J, Deymier PA, Hoying JB, Wong PK. Mechanically induced intercellular

calcium communication in confined endothelial structures. Biomaterials. 2013;34(8):2049-56.

PMCID: 3542404.

27. Boyd NL, Nunes SS, Krishnan L, Jokinen JD, Ramakrishnan VM, Bugg AR, and Hoying JB.

Dissecting the Role of Human Embryonic Stem Cell-Derived Mesenchymal Cells in Human

Umbilical Vein Endothelial Cell Network Stabilization in Three-Dimensional Environments. Tissue

Eng Part A 2013. 19(1-2):211-23.

28. Long J, Junkin M, Wong PK, Hoying J, and Deymier P. Calcium wave propagation in networks of

endothelial cells: model-based theoretical and experimental study. PLoS Comput Biol.

2012;8(12):e1002847.

29. Leblanc AJ, Krishnan L, Sullivan CJ, Williams SK, and Hoying JB. Microvascular Repair: Post-

Angiogenesis Vascular Dynamics. Microcirculation. 2012. 19(8):676-95.

30. Gruionu G, Hoying JB, Pries AR, and Secomb TW. Structural Remodeling of the Mouse Gracilis

Artery: Coordinated Changes in Diameter and Medial Area Maintain Circumferential Stress.

Microcirculation. 2012. 19(7):610-8.

31. LeBlanc AJ, Touroo JS, Hoying JB, and Williams SK. Adipose stromal vascular fraction cell

construct sustains coronary microvascular function after acute myocardial infarction. Am J Physiol

Heart. 2012. 302(4):H973-82.

32. Chang CC, Krishnan L, Nunes SS, Church KH, Edgar LT, Boland ED, Weiss JA, Williams SK,

Hoying JB. Determinants of Microvascular Network Topologies in Implanted Neovasculatures.

2012. Arterioscler. Thromb. Vasc. Biol. 32(1):5-14.

33. Stabenfeldt SE, Gourley M, Krishnan L, Hoying JB, and Barker TH. Engineering fibrin polymers

through engagement of alternative polymerization mechanisms. Biomaterials. 2012 Jan,33(2):535-

44.

34. Cardinal TR, Struthers KR, Kesler TJ, Yocum MD, Kurjiaka DT, and Hoying JB. Chronic hindlimb

ischemia impairs functional vasodilation and vascular reactivity in mouse feed arteries. 2011. Front.

Physio. 2:91.

35. Nunes SS, Rekapally H, Chang CC, and Hoying JB. Vessel arterial-venous plasticity in adult

neovascularization. PLoS One. 2011. 6(11):e27332.

36. Krishnan L, Clayton LR, Boland ED, Reed RM, Hoying JB, and Williams SK. Cellular

immunoisolation for islet transplantation by a novel dual porosity electrospun membrane.

Transplant Proc. 43(9):3256-61. 2011.

37. Boyd NL, Nunes SS, Jokinen JD, Krishnan L, Chen Y, Smith KH, Stice SL, Hoying JB.

Microvascular Mural Cell Functionality of Human Embryonic Stem Cell-Derived Mesenchymal

Cells. Tissue Eng Part A. 2011. 17(11-12):1537-48.

38. Chang CC, Boland ED, Williams SK, and Hoying JB. Direct-write Bioprinting Three-Dimensional

Biohybrid Systems for Future Regenerative Therapies. J Biomed. Mat. Res. A. 2011. 98(1):160-70.


41

39. Myers SA, Devries WH, Sorg KR, Gruenthal MJ, Benton RL, Hoying JB, Hagg T, Whittemore SR.

CD47 knockout mice exhibit improved recovery from spinal cord injury. Neurobiol Dis. 2011

42(1):21-34.

40. Diamond-Stanic MK, Romero-Aleshire MJ, Hoyer PB, Greer K, Hoying JB, Brooks HL. Midkine,

a heparin-binding protein, is increased in the diabetic mouse kidney postmenopause. Am J Physiol

Renal Physiol. 2011. 300(1):F139-46.

41. Gruionu, G, Stone AL, Schwartz MA, Hoying JB, and Williams SK. Gruionu, G, Stone AL,

Schwartz MA, Hoying JB, and Williams SK. Encapsulation of ePTFE in prevascularized collagen

leads to peri-implant vascularization with reduced inflammation. J. Biomedical Materials Research

A. 2010. 95(3):811-8.

42. Nunes SS, Krishnan L, Gerard CS, Dale JR, Maddie MA, Benton RL, and Hoying JB. Angiogenic

Potential of Microvessel Fragments is Independent of the Tissue of Origin and can be Influenced by

the Cellular Composition of the Implants. Microcirculation. 2010. 17(7): 557–567.

43. Bernas MJ, Cardoso FL, Daley SK, Weinand ME, Campos AR, Ferreira AJ, Hoying JB, Witte MH,

Brites D, Persidsky Y, Ramirez SH, Brito MA. Establishment of primary cultures of human brain

microvascular endothelial cells to provide an in vitro cellular model of the blood-brain barrier. Nat

Protocols. 2010 5(7):1265-72.

44. Deymier PA, Runge K, Deymier MJ, Hoying JB, Vasseur JO. Calcium wave propagation in chains

of endothelial cells with nonlinear reaction dynamics: Green's function approach. Phys Rev E Stat

Nonlin Soft Matter Phys. 2010. 82(4 Pt 1):041913.

45. Deymier PA, Eray M, Deymier MJ, Runge K, Hoying JB, Vasseur JO. Architecture-dependent

signal conduction in model networks of endothelial cells. Phys.Rev.E.Stat.Nonlin.Soft.Matter Phys.

2010 Apr,81(4 Pt 1):041915

46. Chang CC, Nunes SS, Sibole SC, Krishnan L, Williams SK, Weiss JA, Hoying JB. Angiogenesis in

a microvascular construct for transplantation depends on the method of chamber circulation. Tissue

Eng Part A 2010 Mar,16(3):795-805. PMCID:PMC2862615

47. Nunes SS, Greer KA, Stiening CM, Chen HY, Kidd KR, Schwartz MA, Sullivan CJ, Rekapally H,

Hoying JB. Implanted microvessels progress through distinct neovascularization phenotypes.

Microvasc.Res. 2010 Jan,79(1):10-20. PMCID:PMC2813398

48. Rhoads RP, Johnson RM, Rathbone CR, Liu X, Temm-Grove C, Sheehan SM, Hoying JB, Allen

RE. Satellite cell-mediated angiogenesis in vitro coincides with a functional hypoxia-inducible

factor pathway. Am.J.Physiol Cell Physiol 2009 Jun,296(6):C1321-C1328. PMCID:PMC2692418

49. Valenzuela K, Raghavan S, Deymier PA, Hoying J. Formation of copper nanowires by electroless

deposition using microtubules as templates. J.Nanosci.Nanotechnol. 2008 Jul,8(7):3416-21

50. Fernandez SM, Keating AF, Christian PJ, Sen N, Hoying JB, Brooks HL, Hoyer PB. Involvement

of the KIT/KITL signaling pathway in 4-vinylcyclohexene diepoxide-induced ovarian follicle loss

in rats. Biol.Reprod. 2008 Aug,79(2):318-27. PMCID:PMC2714991


42

51. Krishnan L, Underwood CJ, Maas S, Ellis BJ, Kode TC, Hoying JB, Weiss JA. Effect of

mechanical boundary conditions on orientation of angiogenic microvessels. Cardiovasc.Res. 2008

May 1,78(2):324-32

52. Stiening CM, Hoying JB, Abdallah MB, Hoying AM, Pandey R, Greer K, Collier RJ. The effects of

endocrine and mechanical stimulation on stage I lactogenesis in bovine mammary epithelial cells.

J.Dairy Sci. 2008 Mar,91(3):1053-66.

53. Eray M, Deymier P.A., Hoying JB, Runge K, and Vasseur JO. Resonant filtering of compositional

waves in multicellular networks. 2008. Physica D 237:2777- 2786.

54. Castro JS, Trzaskowski B, Deymier PA, Bucay J, Adamowicz L, and Hoying JB. Binding Affinity

of Fluorochromes and Fluorescent Proteins to Taxol Crystals. 2008. J. Mat. Sci. Engr. C. 29, 1609-

1615.

55. Castro JS, Deymier PA, Smith SD, and Hoying JB. Morphology of Microtubules Grown in Agarose

Gels. 2008. Advanced Materials 20:183-188.

56. Hiscox AM, Stone AL, Limesand S, Hoying JB, Williams SK. An Islet-Stabilizing Implant

Constructed Using a Preformed Vasculature. Tissue Eng 2007 Dec 21,

57. Maitra N, Adamson C, Greer K, Klewer S, Hoying J, Bahl JJ, Goldman S, Morkin E. Regulation of

gene expression in rats with heart failure treated with the thyroid hormone analog 3,5-

diiodothyropropionic acid (DITPA) and the combination of DITPA and captopril.

J.Cardiovasc.Pharmacol. 2007 Nov,50(5):526-34

58. Krishnan L, Hoying JB, Nguyen H, Song H, Weiss JA. Interaction of angiogenic microvessels with

the extracellular matrix. Am.J.Physiol Heart Circ.Physiol 2007 Dec,293(6):H3650-H3658

59. Cai Q, McReynolds MR, Keck M, Greer KA, Hoying JB, Brooks HL. Vasopressin receptor subtype

2 activation increases cell proliferation in the renal medulla of AQP1 null mice. Am.J.Physiol Renal

Physiol 2007 Dec,293(6):F1858-F1864

60. Shepherd BR, Hoying JB, Williams SK. Microvascular transplantation after acute myocardial

infarction. Tissue Eng 2007 Dec,13(12):2871-9

61. Pysher MD, Sollome JJ, Regan S, Cardinal TR, Hoying JB, Brooks HL, Vaillancourt RR. Increased

hexokinase II expression in the renal glomerulus of mice in response to arsenic.

Toxicol.Appl.Pharmacol. 2007 Oct 1,224(1):39-48. PMCID:PMC2042004

62. Cardinal TR, Hoying JB. A modified fluorescent microsphere-based approach for determining

resting and hyperemic blood flows in individual murine skeletal muscles. Vascul. Pharmacol. 2007

Jul,47(1):48-56. PMCID:PMC2041860

63. Smith CM, Cole SJ, Williams SK, Rodriguez JJ, Hoying JB. Automatic thresholding of three-

dimensional microvascular structures from confocal microscopy images. J.Microsc. 2007

Mar,225(Pt 3):244-57

64. Kirkpatrick ND, Andreou S, Hoying JB, Utzinger U. Live imaging of collagen remodeling during

angiogenesis. Am.J.Physiol Heart Circ.Physiol 2007 Jun,292(6):H3198-H3206


43

65. Chakraborty A, Brooks H, Zhang P, Smith W, McReynolds MR, Hoying JB, Bick R, Truong L,

Poindexter B, Lan H, et al. Stanniocalcin-1 regulates endothelial gene expression and modulates

transendothelial migration of leukocytes. Am.J.Physiol Renal Physiol 2007 Feb,292(2):F895-F904.

66. Ramos RF, JB Hoying, Witte MH, Daniel Stamer W. 2007. Schlemm's canal endothelia, lymphatic,

or blood vasculature? J Glaucoma. 16(4):391-405.

67. Kirkpatrick ND, Hoying JB, Botting SK, Weiss JA, Utzinger U. In vitro model for endogenous

optical signatures of collagen. J.Biomed.Opt. 2006 Sep,11(5):054021

68. Chang CC, Hoying JB. Directed three-dimensional growth of microvascular cells and isolated

microvessel fragments. Cell Transplant. 2006,15(6):533-40

69. Calaluce R, Beck SK, Bair EL, Pandey R, Greer KA, Hoying AM, Hoying JB, Mount DW, Nagle

RB. Human laminin-5 and laminin-10 mediated gene expression of prostate carcinoma cells.

Prostate 2006 Sep 15,66(13):1381-90

70. Greer KA, McReynolds MR, Brooks HL, Hoying JB. CARMA: A platform for analyzing

microarray datasets that incorporate replicate measures. BMC.Bioinformatics. 2006,7:149.

PMCID:PMC1450302

71. Cai Q, Keck M, McReynolds MR, Klein JD, Greer K, Sharma K, Hoying JB, Sands JM, Brooks

HL. Effects of water restriction on gene expression in mouse renal medulla: identification of

3betaHSD4 as a collecting duct protein. Am.J.Physiol Renal Physiol 2006 Jul,291(1):F218-F224

72. Yang Y, Deymier PA, Wang L, Guzman R, Hoying JB, McLaughlin HJ, Smith SD, Jongewaard IN.

Nucleation and growth of microtubules from gamma-tubulin-functionalized gold surfaces.

Biotechnol.Prog. 2006 Jan,22(1):303-12

73. Guilkey JE, Hoying JB, Weiss JA. Computational modeling of multicellular constructs with the

material point method. J.Biomech. 2006,39(11):2074-86

74. Vartanian KB, Chen HY, Kennedy J, Beck SK, Ryaby JT, Wang H, Hoying JB. The non-

proteolytically active thrombin peptide TP508 stimulates angiogenic sprouting. J.Cell Physiol 2006

Jan,206(1):175-80

75. Shiu YT, Weiss JA, JB Hoying, Iwamoto MN, Joung IS and Quam CT. The role of mechanical

stresses in angiogenesis. Crit Rev Biomed Eng 33: 431-510, 2005.

76. Yang Y, Guzman R, Deymier PA, Umnov M, Hoying J, Raghavan S, Palusinski O, Zelinski BJ.

Adsorption of a microtubule on a charged surface affects its disassembly dynamics.

J.Nanosci.Nanotechnol. 2005 Dec,5(12):2050-6

77. Kidd KR, Dal PD, Stone AL, Hoying JB, Nagle RB, Williams SK. Stimulated endothelial cell

adhesion and angiogenesis with laminin-5 modification of expanded polytetrafluoroethylene. Tissue

Eng 2005 Sep,11(9-10):1379-91

78. Deymier PA, Yang Y, Hoying J. Effect of tubulin diffusion on polymerization of microtubules.

Phys.Rev.E.Stat.Nonlin.Soft.Matter Phys. 2005 Aug,72(2 Pt 1):021906

79. Schwartz MA, Stone AL, Greer KA, Hoying JB, Williams SK. Gene expression in tissue associated

with extracellular matrix modified ePTFE. J.Biomed.Mater.Res.A 2005 Apr 1,73(1):30-8


44

80. Gruionu G, Hoying JB, Gruionu LG, Laughlin MH, Secomb TW. Structural adaptation increases

predicted perfusion capacity after vessel obstruction in arteriolar arcade network of pig skeletal

muscle. Am.J.Physiol Heart Circ.Physiol 2005 Jun,288(6):H2778-H2784

81. Gruionu G, Hoying JB, Pries AR, Secomb TW. Structural remodeling of mouse gracilis artery after

chronic alteration in blood supply. Am.J.Physiol Heart Circ.Physiol 2005 May,288(5):H2047-

H2054

82. McReynolds MR, Taylor-Garcia KM, Greer KA, Hoying JB, Brooks HL. Renal medullary gene

expression in aquaporin-1 null mice. Am.J.Physiol Renal Physiol 2005 Feb,288(2):F315-F321

83. Brewer MA, Utzinger U, Barton JK, Hoying JB, Kirkpatrick ND, Brands WR, Davis JR, Hunt K,

Stevens SJ, Gmitro AF. Imaging of the ovary. Technol.Cancer Res.Treat. 2004 Dec,3(6):617-27

84. Adamson C, Maitra N, Bahl J, Greer K, Klewer S, Hoying J, Morkin E. Regulation of gene

expression in cardiomyocytes by thyroid hormone and thyroid hormone analogs 3,5-

diiodothyropropionic acid and CGS 23425 [N-[3,5-dimethyl-4-(4'-hydroxy-3'-isopropylphenoxy)-

phenyl]-oxamic acid]. J.Pharmacol.Exp.Ther. 2004 Oct,311(1):164-71

85. Krishnan L, Weiss JA, Wessman MD, Hoying JB. Design and application of a test system for

viscoelastic characterization of collagen gels. Tissue Eng 2004 Jan,10(1-2):241-52

86. Shepherd BR, Chen HY, Smith CM, Gruionu G, Williams SK, Hoying JB. Rapid perfusion and

network remodeling in a microvascular construct after implantation.

Arterioscler.Thromb.Vasc.Biol. 2004 May,24(5):898-904

87. Sullivan CJ, Doetschman T, Hoying JB. Targeted disruption of the Fgf2 gene does not affect

vascular growth in the mouse ischemic hindlimb. J.Appl.Physiol 2002 Dec,93(6):2009-17

88. Sullivan CJ, Hoying JB. Flow-dependent remodeling in the carotid artery of fibroblast growth

factor-2 knockout mice. Arterioscler.Thromb.Vasc.Biol. 2002 Jul 1,22(7):1100-5

89. Barton JK, Hoying JB, Sullivan CJ. Use of microbubbles as an optical coherence tomography

contrast agent. Acad.Radiol. 2002 May,9 Suppl 1:S52-S55

90. Schultz JJ, Hoying JB, Sullivan CJ, Doetschman T. FGF and the cardiovascular system. In: Cuevas

P, editor. Fibroblast growth factor in the cardiovascular system. I. Holzapfel, 2001.

91. Carter WB, Hoying JB, Boswell C, Williams SK. HER2/neu over-expression induces endothelial

cell retraction. Int.J.Cancer 2001 Feb 1,91(3):295-9

92. Carter WB, Uy K, Ward MD, Hoying JB. Parathyroid-induced angiogenesis is VEGF-dependent.

Surgery 2000 Sep,128(3):458-64

93. Hoying JB, Yin M, Diebold R, Ormsby I, Becker A, Doetschman T. Transforming growth factor

beta1 enhances platelet aggregation through a non-transcriptional effect on the fibrinogen receptor.

J.Biol.Chem. 1999 Oct 22,274(43):31008-13

94. Engle SJ, Hoying JB, Boivin GP, Ormsby I, Gartside PS, Doetschman T. Transforming growth

factor beta1 suppresses nonmetastatic colon cancer at an early stage of tumorigenesis. Cancer Res.

1999 Jul 15,59(14):3379-86


45

95. Sutliff RL, Hoying JB, Kadambi VJ, Kranias EG, Paul RJ. Phospholamban is present in endothelial

cells and modulates endothelium-dependent relaxation. Evidence from phospholamban gene-

ablated mice. Circ.Res. 1999 Feb 19,84(3):360-4

96. Zhou M, Sutliff RL, Paul RJ, Lorenz JN, Hoying JB, Haudenschild CC, Yin M, Coffin JD, Kong L,

Kranias EG, et al. Fibroblast growth factor 2 control of vascular tone. Nat.Med. 1998 Feb,4(2):201-

7

97. Liu LH, Paul RJ, Sutliff RL, Miller ML, Lorenz JN, Pun RY, Duffy JJ, Doetschman T, Kimura Y,

MacLennan DH, et al. Defective endothelium-dependent relaxation of vascular smooth muscle and

endothelial cell Ca2+ signaling in mice lacking sarco(endo)plasmic reticulum Ca2+-ATPase

isoform 3. J.Biol.Chem. 1997 Nov 28,272(48):30538-45

98. Hoying JB, Williams SK. Effects of basic fibroblast growth factor on human microvessel

endothelial cell migration on collagen I correlates inversely with adhesion and is cell density

dependent. J.Cell Physiol 1996 Aug,168(2):294-304

99. Hoying JB, Boswell CA, Williams SK. Angiogenic potential of microvessel fragments established

in three-dimensional collagen gels. In Vitro Cell Dev.Biol.Anim 1996 Jul,32(7):409-19

100. Kleinert LB, Hoying JB, Williams SK. The neointima formed in endothelial cell sodded ePTFE

vascular grafts results from both cellular-hyperplasia and extracellular-hypertrophy. Cell

Transplant. 1996 Jul,5(4):475-82

101. Hoying JB, Williams SK. Measurement of endothelial cell migration using an improved linear

migration assay. Presented at the 1995 Microcirculatory Society Meeting. Microcirculation. 1996

Jun,3(2):167-74

102. Hoying JB, Chen SC, Williams SK. Interaction of colloidal gold-labelled glucosylated albumin

with endothelial cell monolayers: comparison between cryofixation and glutaraldehyde fixation.

Microsc.Res.Tech. 1995 Feb 15,30(3):252-7

103. Wu P, Hoying JB, Williams SK, Kozikowski BA, Lauffenburger DA. Integrin-binding peptide

in solution inhibits or enhances endothelial cell migration, predictably from cell adhesion.

Ann.Biomed.Eng 1994 Mar,22(2):144-52

104. Hoying JB, Williams SK. Endothelial cell monolayers viewed by TIRF microscopy. In Vitro

Cell Dev.Biol.Anim 1994 Jan,30A(1):1-3

105. Young C, Jarrell BE, Hoying JB, Williams SK. A porcine model for adipose tissue-derived

endothelial cell transplantation. Cell Transplant. 1992,1(4):293-8

106. Hoying JB, Behm SM, Lang-Unnasch N. Cloning and characterization of

Rhizobium meliloti loci required for symbiotic root nodule invasion. Mol.Plant Microbe Interact.

1990 Jan,3(1):18-27.

Manuscripts in Review/ Revision/Preparation.


46

Jacob R. Dale JR, Breite AG, Clayton L, Dwulet FE, McCarthy RC, McCurry T, Williams SK, and

Hoying JB. Impact of Enzyme Composition on Stromal Vascular Fraction Cell Isolation from Adipose

Tissue. Cell Transplantation. in review.

Adeyemo A, Zhang Y, Doetschman T, Hoying JB, and Schultz JEJ. High molecular weight isoforms of

fibroblast growth factor-2 are sufficient and necessary for post-ischemic revascularization and recovery

of the mouse hindlimb. ATVB. Revision in review

Krishnan L, Nunes SS, Chang CC, Clayton L, Williams SK, and Hoying JB. Invasion of tissue

interfaces by angiogenic neovessels requires stromal cells and is VEGF-dependent. PLoS One. in

preparation

Krishnan L, Chang CC, Rekapally H, Williams SK, and Hoying JB. Insulinemia and not hyperglycemia

impairs vascular sprouting in a model of adipose angiogenesis. Cardiovasc. Diabetol. in preparation

Books and Book Chapters

Hoying JB, Dale J, Touroo J, Howard A, Chang CC, and Williams SK. Direct-write Bioprinting of a

3D Vascularized Tissue Model. In Dudley A, Ed. Methods in Organogenesis; Methods in

Molecular Biology series. Humana Press. 2016. in press.

Williams SK and Hoying JB. Bioinks for Bioprinting. In: Bioprinting in Regenerative Medicine

Springer. 2015.

Hoying JB and Williams SK. Biofabrication of Vascular Networks. In Atala A and Yoo JJ, Eds. 3D

Biofabrication for Biomedical and Translational Research. Elsevier, 2015.

Williams SK and Hoying JB. Adipose Stromal Vascular Fraction Cells for Vascularization of

Engineered Tissues. In Brey EM, Ed. Vascularization: Regenerative Medicine and Tissue

Engineering. CRC Press, 2014.

Keller BB, Hoying JB, Markwald RR. Molecular Development of the Heart. In Furster V, Walsh RA

and Harrington RA, Eds. Hurst’s The Heart, Thirteenth Edition. The McGraw-Hill Companies, Inc.

2011.

Hoying JB and Williams SK. Building blood vessels. In Aird W.C, Ed. Endothelial Biomedicine.

Cambridge: Cambridge University Press, 2007.

Hoying JB, editor. Genetics of Angiogenesis. United Kingdom: BIOS Scientific Publishers Ltd. 2003.

Hoying JB, KR. Kidd and CJ Sullivan. Heterogeneity of Angiogenesis. Genetics of Angiogenesis.

Hoying (ed.), United Kingdom: BIOS Scientific Publishers Ltd. 2003, ppg. 191-203.

Williams SK and JB Hoying. Angiogenesis and Biomaterials. Encyclopedia of Biomaterials and

Biomedical Engineering. Wenk G and Bowlin G, eds, Marcel Dekker, Inc. Publishers. New York,

NY. 2002.


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Schultz, JJ, JB Hoying, CJ Sullivan, and T Doetschman. 2001. FGF and the cardiovascular system. In

Fibroblast growth factor in the cardiovascular system. P.Cuevas, editor. I. Holzapfel, Publisher.

hoying, james b - cardiovascular innovation institute (cii) · hoying, james b january, 2016 1...

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