hplc system site planning guide - sciex

HPLC System

Site Planning Guide

May 2015RUO-IDV-01-1354-C | D5102066 A

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Site Planning GuideHPLC SystemRUO-IDV-01-1354-C | D5102066 A2 of 31

Chapter 1 Introduction...............................................................................................................................4Customer Site Planner Responsibilities..............................................................................................................................4FSE Responsibilities............................................................................................................................................................4Technical Support..............................................................................................................................................................5

Chapter 2 Site Planning Checklist..............................................................................................................6Customer Information........................................................................................................................................................6Requirements.....................................................................................................................................................................6

Site Layout Requirements............................................................................................................................................6Electrical Requirements................................................................................................................................................7Ventilation and Waste Collection Requirements..........................................................................................................7Environmental Requirements.......................................................................................................................................8Computer and Network Requirements.........................................................................................................................8Solutions and Equipment Requirements......................................................................................................................8Product Familiarization................................................................................................................................................9

Comments..........................................................................................................................................................................9Exceptions..........................................................................................................................................................................9Signoff...............................................................................................................................................................................9

Appendix A Site Requirements................................................................................................................10Site Layout Requirements................................................................................................................................................10

Laboratory Layout and Clearances.............................................................................................................................10Module Stacking........................................................................................................................................................11Weights and Dimensions...........................................................................................................................................18

Electrical Requirements....................................................................................................................................................21Surge Suppression......................................................................................................................................................21Protective Earth Conductor........................................................................................................................................22System Electrical Specifications..................................................................................................................................22

Ventilation and Waste Collection Requirements..............................................................................................................25Ventilation Requirements...........................................................................................................................................25Waste Collection Requirements.................................................................................................................................26

Environmental Requirements...........................................................................................................................................27BioSafety Requirements.............................................................................................................................................27

Computer and Network Requirements.............................................................................................................................27Customer Supplied Solutions and Equipment..................................................................................................................29

Appendix B Equipment Safety Categories..............................................................................................30

Revision History........................................................................................................................................31

HPLC SystemSite Planning Guide3 of 31RUO-IDV-01-1354-C | D5102066 A

Contents

This guide is for the site planner, the individual responsible for preparing the facility for the installation of theHPLC system .

Customer Site Planner ResponsibilitiesComplete the Site Planning Checklist on page 6, in consultation with facilities services personnel (electrical,ventilation, and information technology [IT]), and return it to the FSE before the completion date. Refer to Signoffon page 9.

Note: The FSE will follow up if the checklist is not received prior to the scheduled installation date.

• Verify that adequate space and the required shipping facilities are available. Refer to Site LayoutRequirements on page 10.

• Provide all required electrical receptacles. Refer to Electrical Requirements on page 21.

• Provide and install all required vents and ventilation devices. Refer to Ventilation and Waste CollectionRequirements on page 25.

• Verify that the requirements for the operating environment are met. Refer to Environmental Requirementson page 27.

• Provide all required solutions and laboratory equipment, including all fittings, and sample tubing for the liquidchromatography (LC) equipment, unless purchased from SCIEX . Refer to Customer Supplied Solutionsand Equipment on page 29.

• When the shipment arrives, inspect the packaging for damage and then contact SCIEX Customer Service orthe local FSE to schedule the installation.

FSE Responsibilities• Review the checklist and discuss outstanding issues with the site planner.

• Unpack and set up optional LC equipment sold and supported by SCIEX .

• Test and qualify the system to the specifications in the Data Log.

During installation, the FSE provides a system and software overview, reviews data, and provides some basicoperator familiarization.


1Introduction

Technical SupportAB SCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughoutthe world. They can answer questions about the system or any technical issues that might arise. For moreinformation, visit the Web site at sciex.com.


Introduction

http://sciex.com

Customer Information

Organization

Address

City

Country

Zip/Postal codeTelephone

FaxSite planner contactname

E-mail address

Requirements

Site Layout RequirementsRefer to Site Layout Requirements on page 10.

CompleteRequirement

The measured building clearances can accommodate the equipment and crate dimensions.

If the requirements cannot be met, then contact the FSE.


2Site Planning Checklist

Electrical RequirementsRefer to Electrical Requirements on page 21.

N/ACompleteRequirement

Installation of electrical supplies and fixtures complies with local regulations andsafety standards.

On branch circuit is provided for the HPLC system with an AC mains supply outletfor each component of the system.

Note: Do not use extension cords.

A 20 A (minimum) circuit breaker is provided for the HPLC system powerconnectors.

All modules are connected to a high-capacity electrical surge suppressor.

The AC mains supply voltage source does not fluctuate more than ±10% fromthe nominal voltage.

If the voltage is not in the recommended range, a power line regulator is available.

The mains supply includes a correctly installed protective earth conductor.

A qualified electrician has determined the appropriate AC mains supplyconfiguration based on the system electrical specifications found in SystemElectrical Specifications on page 22.

Ventilation and Waste Collection RequirementsRefer to Ventilation and Waste Collection Requirements on page 25.

CompleteRequirement

Installation of plumbing and ventilation fixtures complies with local regulations and safetystandards.

Ventilation of the laboratory environment in which the HPLC system will be used complieswith local regulations and the air exchange rate is appropriate for the work performed.

Primary and secondary waste containers are available to collect liquid waste.

Note: Liquid waste containers must be positioned below and close to the liquid waste portfor gravity flow.


Site Planning Checklist

Environmental RequirementsRefer to Environmental Requirements on page 27.

CompleteRequirement

The altitude does not exceed 1 828 m (6 000 ft) above sea level.

Temperature and humidity requirements have been met.

BioSafety Requirements

Refer to BioSafety Requirements on page 27.

CompleteRequirement

The site is not designated as BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4).

Computer and Network RequirementsRefer to Computer and Network Requirements on page 27.


A computer name is available.

The default IP addresses and subnet masks are compatible with the customerLAN.

An active, tested LAN connection is available.

Network hardware is compatible with an RJ45-type connector.

If the network connection is more than 2 m (6 feet) from the system, a Category5 RJ45 Ethernet cable of the required length is available.

If the system contains a VWD detector or other unsupported components, a GPIBor ADC card is available.

Solutions and Equipment RequirementsRefer to Customer Supplied Solutions and Equipment on page 29.

CompleteRequirement

All required solutions and bottles are available.

All required LC equipment and supplies are available.



Product Familiarization


An account has been obtained on the SCIEX Learning Portal, and the onlinelearning modules have been completed.Go to sciex.com/LearningPortal and then click the link to request an account.When you receive your account information, complete the online learning modules.

System documentation has been obtained and reviewed. Go to sciex.com/products.

Comments

Exceptions

Signoff

Completion date(yyyy-mm-dd)

Site planner contact name

I acknowledge that all of the installation requirements, as specified in this document, havebeen met.

Return date(yyyy-mm-dd)

FSE name

FSE e-mail



http://sciex.com/LearningPortal

http://sciex.com/products

Site Layout RequirementsReturn to checklist.

Laboratory Layout and ClearancesReturn to checklist.

• Avoid installing the system beside heaters or cooling ducts, or in direct sunlight.

• Install the system away from vibrating equipment, such as refrigerators or centrifuges. The system must notshare bench space with vibrating equipment.

• Install the computer within 2 m (6 feet) of the instrument, with a LAN or RS232 cable control.

• Allow 46 cm (18 inches) of free space behind the system, for service access to the rear of the instrument.

Figure A-1 Required Access Space Behind the HPLC Bench

DescriptionItem

HPLC table1

Mass spectrometer2

Distance to wall, 46 cm (18 inches)3

• Allow 3 cm between modules when they are stacked side-by-side.


ASite Requirements

• Position the monitor, keyboard, and accessories to allow for proper ergonomics during use.

• Store chemicals in a secondary containment system at a convenient height for handling, preferably below eyelevel.

• (Recommended) Install the system on a wheeled bench.

Module StackingReturn to checklist.

Provide a bench for the HPLC system that can support the total weight of all modules and solvents, and that isstable and flat.

Refer to Weights and Dimensions on page 18 for dimensions and weights.

Refer to Figure A-2 to Figure A-7 for example stacking configurations.

Note: Install and operate all HPLC system modules in a horizontal position. Operating a module on its side canimpair the leak detection function and possibly cause a hardware failure within the module.

Note: The thermostatted versions of all Agilent autosamplers include the G1330A/B thermostat module. Thethermostat module must be placed directly under the autosampler to be thermostatted. We recommend thatthe thermostat module be positioned as the bottom module of the stack, directly on the laboratory bench.

Agilent systems can use a single-stack configuration only if:• The height of the stack does not result in a safety problem.

• The system does not include a G1330 B thermostat module.

A multiple-stack configuration is required if:• The stack of modules is too high, resulting in a safety problem.

• The system includes a G1330 B thermostat module.


Site Requirements

Figure A-2 ExionLC and Shimadzu System Stack Configurations

DescriptionItem

Autosampler1

Column oven2

Detector3

Pump4


Site Requirements

Figure A-3 Agilent 1260 Stack: Configuration 1

DescriptionItem

Detector1

Column compartment2

Autosampler3

Pump4

Instant Pilot5

Vacuum degasser6

Solvent cabinet7


Site Requirements


DescriptionItem

Pump1

Vacuum degasser2

Solvent cabinet3

Thermostat4

Autosampler5

Column compartment6

Instal Pilot7

Detector8


Site Requirements


DescriptionItem

Pump1

Solvent cabinet2

(Optional) Thermostat for the ALS3

Autosampler4

Column compartment5

Instant Pilot6

Detector7


Site Requirements


DescriptionItem

Pump1

Autosampler2

Column compartment3

Detector4

Instant Pilot5

Solvent cabinet6


Site Requirements

Figure A-7 CTC PAL System Configuration

DescriptionItem

Keypad terminal1

Safety guard2

Microplate stack3

Tray holder4

Tray5

Fast wash station6

LC injection valve7

X-axis8

Y-axis9

Injection unit x-axis10

Plunge holder11

Syringe and syringe adapter12

HPLC system (ExionLC, Agilent, or Shimadzu)13


Site Requirements

Weights and DimensionsReturn to checklist.

WeightDepthWidthHeightHPLC SystemModule

ExionLC Systems

58 kg (127.87 lbs)50 cm (19.69 inches)41 cm (16.14 inches)61 cm (24 inches)ExionLC 100 System

5.5 kg (12.13 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)ExionLC Controller

30 kg (66.14 lbs)50 cm (19.69 inches)26 cm (10.24 inches)42 cm (16.34 inches)ExionLC ACAutosampler

23 kg (50.71 lbs)42 cm (16.54 inches)26 cm (10.24 inches)42 cm (16.34 inches)ExionLC AC ColumnOven - 100 V



10 kg (22.05 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)ExionLC AC Pump

33 kg (72.7 lbs)50 cm (19.69 inches)26 cm (10.24 inches)42 cm (16.34 inches)ExionLC ADAutosampler

10 kg (22.05 lbs)50 cm (19.69 inches)26 cm (10.24 inches)21 cm (8.3 inches)ExionLC AD ColumnOven

11.8 kg (26 lbs)50 cm (19.69 inches)26 cm (10.24 inches)14 cm (5.52 inches)ExionLC AD Pump

5 kg (11.02 lbs)42 cm (16.53 inches)26 cm (10.24 inches)7 cm (2.9 inches)ExionLC Degasser

10 kg (22.05 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)ExionLC HPLC Pump

61 kg (134.48 lbs)50 cm (19.69 inches)54 cm (21.27 inches)42 cm (16.34 inches)ExionLC ADMultiplate Sampler

32 kg (70.55 lbs)50 cm (19.69 inches)43 cm (16.73 inches)42 cm (16.54 inches)ExionLC RackChanger

13 kg (28.66 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)ExionLC UV Detector

12 kg (26.5 lbs)50 cm (19.69 inches)26 cm (10.24 inches)14 cm (5.52 inches)ExionLC PDADetector

Shimadzu

5.5 kg (12.13 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)CBM-20A


Site Requirements


20 kg (44.09 lbs)42 cm (16.54 inches)26 cm (10.24 inches)42 cm (16.34 inches)CTO-20A

23 kg (50.71 lbs)42 cm (16.54 inches)26 cm (10.24 inches)42 cm (16.34 inches)CTO-20AC

10 kg (22.05 lbs)50 cm (19.69 inches)26 cm (10.24 inches)21 cm (8.3 inches)CTO-30AC

5 kg (11.02 lbs)42 cm (16.53 inches)26 cm (10.24 inches)7 cm (2.9 inches)DGU-20A3/20A5

5 kg (11.02 lbs)27 cm (10.63 inches)11 cm (4.34 inches)15 cm (6 inches)FCV-20AH2/20AH6

13 kg (28.66 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)LC-20AB/XR

10 kg (22.05 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)LC-20AD

11 kg (25 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)LC-20AT

11.8 kg (26 lbs)50 cm (19.69 inches)26 cm (10.24 inches)14 cm (5.52 inches)LC-30AD

25 kg (55.12 lbs)50 cm (19.69 inches)43 cm (16.73 inches)42 cm (16.54 inches)Rack Changer

32 kg (70.55 lbs)50 cm (19.69 inches)43 cm (16.73 inches)42 cm (16.54 inches)Rack Changer II

27 kg (59.52 lbs)50 cm (19.69 inches)26 cm (10.24 inches)42 cm (16.34 inches)SIL-20A/A XR

30 kg (66.14 lbs)50 cm (19.69 inches)26 cm (10.24 inches)42 cm (16.34 inches)SIL-20AC/AC XR

33 kg (72.7 lbs)50 cm (19.69 inches)26 cm (10.24 inches)42 cm (16.34 inches)SIL-30AC

61 kg (134.5 lbs)50 cm (19.69 inches)54 cm (22 inches)42 cm (16.54 inches)SIL-30ACMP

35 kg (77.16 lbs)50 cm (19.69 inches)26 cm (10.24 inches)42 cm (16.34 inches)SIL-HTA

40 kg (88.18 lbs)50 cm (19.69 inches)26 cm (10.24 inches)42 cm (16.34 inches)SIL-HTC

13 kg (28.66 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)SPD-20A/AV

12 kg (26.46 lbs)42 cm (16.54 inches)26 cm (10.24 inches)14 cm (5.52 inches)SPD-M20A

Agilent 1100/1200/1260

11 kg (25 lbs)44 cm (17 inches)35 cm (13.5 inches)14 cm (5.52 inches)G1310 A / G1311 A/BIsocratic / QuaternaryPumps

15.5 kg (34 lbs)44 cm (17 inches)35 cm (13.5 inches)18 cm (7 inches)G1312 A/BBinary Pump

17 kg (39 lbs)44 cm (17 inches)35 cm (13.5 inches)18 cm (7 inches)G1376 A / G2226 ACapillary/Nano Pump

7.5 kg (16.5 lbs)44 cm (17 inches)35 cm (13.5 inches)8 cm (3 inches)G1322 A / G1379 ADegassers


Site Requirements


14.2 kg (31.3 lbs)44 cm (17 inches)35 cm (13.5 inches)20 cm (8 inches)G1313 A / G1329 A/B/G1389 AAutosamplers

15.5 kg (34.2 lbs)44 cm (17 inches)35 cm (13.5 inches)20 cm (8 inches)G1367 A/B/C/D/EWell Plate Samplers

18.5 kg (40.7 lbs)44 cm (17 inches)35 cm (13.5 inches)15 cm (5.52 inches)G1330 A/BALS Thermostats

10.2 kg (22.5 lbs)44 cm (17 inches)41 cm (16 inches)14 cm (5.52 inches)G1316 A/BThermostattedColumnCompartment

11 kg (25 lbs)44 cm (17 inches)35 cm (13.5 inches)14 cm (5.52 inches)G1314 A/B/CVariable WavelengthDetector

11.5 kg (26 lbs)44 cm (17 inches)35 cm (13.5 inches)14 cm (5.52 inches)G1315 A/B/C/DDiode-ArrayDetectors

7 kg (16 lbs)44 cm (17 inches)35 cm (13.5 inches)8 cm (3 inches)G1379 BMicro Degasser

11.2 kg (24.7 lbs)44 cm (17 inches)35 cm (13.5 inches)14 cm (5.52 inches)G1316 CThermostattedColumnCompartment

Agilent 1290

21.8 kg (48 lbs)44 cm (17 inches)35 cm (13.5 inches)24 cm (9.3 inches)G4220 ABinary Pump

15.5 kg (34.2 lbs)44 cm (17 inches)35 cm (13.5 inches)20 cm (8 inches)G4226 AAutosampler

11.2 kg (24.7 lbs)44 cm (17 inches)35 cm (13.5 inches)14 cm (5.52 inches)G1316 CThermostattedColumnCompartment

11.5 kg (26 lbs)44 cm (17 inches)35 cm (13.5 inches)14 cm (5.52 inches)G4212ADiode-Array Detector


Site Requirements


CTC

8 kg (18 lbs)39 cm (15.2 inches)55 cm (21.5 inches)54 cm (21.0 inches)LC PALwithout accessories

8 kg (18 lbs)39 cm (15.2 inches)55 cm (21.5 inches)65 cm (25.5 inches)HTC PAL/PAL HTC-xtwithout accessories

10 kg (22 lbs)39 cm (15.2 inches)83 cm (32.6 inches)65 cm (25.5 inches)HTS PAL/PAL HTS-xtwithout accessories

Electrical RequirementsReturn to checklist.

WARNING! Electrical Shock Hazard. Use only qualified personnel for the installationof all electrical supplies and fixtures, and make sure that all installations adhere tolocal regulations and safety standards.

• Provide a branch circuit with an AC mains supply outlet for each HPLC system module.

• Provide a 20 A (minimum) circuit breaker for each HPLC system power connector.

• Do not use extension cords.

Note: With the following exceptions, all HPLC system modules have automatic line-sensing with wide rangingpower supplies that operate with line voltages in the range of 100 VAC to 240 VAC ±10%. The ExionLC ACColumn Oven and the Shimadzu CTO-20A/AC are rated for the nominal value shown in Table A-1 on page22. The nominal voltage is manually selected for the ExionLC UV Detector, the ExionLC PDA Detector, and theShimadzu SPD-20A/AV, SPD-M20A, and SPD-M30A.

If the voltage changes more than 10% in 24 hours, use a power conditioner. High or low voltages can adverselyaffect the electronic components of the equipment.

Surge SuppressionReturn to checklist.

The HPLC stack and all of its modules must be plugged into a high capacity electrical surge suppressor, to protectthe system from voltage spikes and power surges. Use a power bar with a built-in surge protector. The maximumcurrent is the sum of the maximum current ratings for all components.

A configuration with two pumps, a degasser, a cooled autosampler, a column oven, and a DAD detector, requiresapproximately 16.5 A.


Site Requirements

Protective Earth ConductorReturn to checklist.

WARNING! Electrical Shock Hazard. Do not intentionally interrupt the protectiveearth conductor. Any interruption of the protective earth conductor will create apotential electric shock hazard.

The mains supply must include a correctly installed protective earth conductor that must be installed or checkedby a qualified electrician before the system is connected.

System Electrical SpecificationsReturn to checklist.

Note: Maximum Current assumes a voltage of 100V.

Maximum PowerConsumption (VA)

Maximum Current (A)HPLC System Module

ExionLC Systems

6006ExionLC 100 System

4004ExionLC Controller (using AC OUT)

1001ExionLC Controller (not using ACOUT)

3003ExionLC AC Autosampler

5005ExionLC AC Column Oven - 100 V

6005ExionLC AC Column Oven - 120 V

6002.7ExionLC AC Column Oven - 240 V

1501.5ExionLC AC Pump

3003ExionLC AD Autosampler

3003ExionLC AD Column Oven

1501.5ExionLC AD Pump

N/AN/AExionLC Degasser

1501.5ExionLC HPLC Pump

3503.5ExionLC Rack Changer


Site Requirements



5505.5ExionLC AD Multiplate Sampler

1601.6ExionLC UV Detector

1501.5ExionLC PDA Detector

Shimadzu

1501.5LC-20AD/AT

1501.5LC-30AD

1801.8LC-20AB

1601.6SPD-20A

1001SIL-20A

3003SIL-20AC/SIL-30AC

5505.5SIL-30ACMP

1501.5SIL-HTA

3003SIL-HTC

1501.5SPD-M20A

4004CBM-20A (using AC OUT)

1001CBM-20A (not using AC OUT)

5005CTO-20A

5505.5CTO-20AC

3003CTO-30AC

500.5Rack Changer

3503.5Rack Changer II

1001FCV-20AH2/20AH6

N/AN/ADGU-20A3/20A5

Agilent 1260

1881.8G1310B Isocratic Pump

1881.8G1311B QuaterN/Ary Pump

742.2G1312 BiN/Ary Pump


Site Requirements



1301.6G4212B Diode Array Detector

852.2G1314F Variable WavelengthDetector

1503.5G1316A Thermostatted ColumnCompartment

30N/AG1322A Degasser

300.3G1379B Micro Degasser

2003.0G1329B Standard Autosampler

2003.0G1367E High PerformanceAutosampler

6N/AG4208A Instant Pilot

Agilent 1290

2702.7G4220A Binary Pump

2002G4226A Autosampler

1501.5G1316C Thermostatted ColumnCompartment

1301.5G4212A Diode Array Detector

CTC

2506.3LC PAL

2506.3HTC PAL/PAL HTC-xt

2506.3HTS PAL/PAL HTS-xt


Site Requirements

Ventilation and Waste Collection RequirementsReturn to checklist.

WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Use onlyqualified personnel for the installation of plumbing and ventilation fixtures,and make sure that all installations follow local bylaws and regulations.

Any liquid waste must drain from the liquid waste port through the supplied tubing to a polyethylene bottle in asecondary container.

The supplied tubing must be used to vent vapors from the liquid waste collection vessel to a suitable fume hoodor fume duct.

Ventilation RequirementsReturn to checklist.

WARNING! Fire and Toxic Chemical Hazard. Make sure that the laboratory inwhich the system operates is well ventilated. Solvents used in highperformance liquid chromatography are flammable and toxic.

WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Make surethat the laboratory is equipped with adequate ventilation to maintain solventvapor within local occupational exposure limits. The use of concentratedorganic solvents as part of a rinse protocol may release solvent vapor in excessof occupational exposure limits.

The venting of fumes and disposal of waste must be in accordance with all federal, state, provincial, and localhealth and safety regulations, and the air exchange rate must be appropriate for the work performed.

Note: In the United States, OSHA 29 CFR Part 1910-1450 requires 4 to 12 air changes per hour in laboratories.


Site Requirements

Waste Collection RequirementsReturn to checklist.

WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Nevercollect or store waste in a glass container. Secure waste bottles in a low-densitypolyethylene safety container, with the cover fastened and the handles lockedin the upright position. Wear appropriate eyewear, clothing, and gloves whenhandling reagent and waste bottles. Glass containers might break or shatter.Reagent and waste bottles might crack and leak.

WARNING! Environmental Hazard. Do not dispose of system components in municipalwaste. Follow established procedures when disposing of components.

Liquid waste exits from the liquid waste port on the HPLC system. To collect the liquid waste, connect the suppliedwaste line from the instrument liquid waste port to a waste-collection vessel. The vessel location must allow theliquid to drain from the port by gravity.

Before connecting the liquid waste port to the liquid waste collection vessel, verify that:

• The shortest and the straightest possible run of polypropylene tubing is used. Tubing length should not exceed2 m (6 ft).

• The tubing drops vertically and does not have low points that could prevent liquid from accumulating andblocking the flow.

• The tubing is securely fastened to the liquid waste port using the fasteners provided with the instrument. Donot use brass fasteners, which can corrode, and take care not to puncture the tubing.

• The tubing is securely attached to the waste-collection vessel.

• The tubing is kept away from sources of potential damage, such as heat, flame, or points of contact (rubbing)with other objects.

• The collection vessel is contained within a secondary vessel.


Site Requirements

Environmental RequirementsReturn to checklist.

WARNING! Fire Hazard. Do not operate the system in the presence of an open flame,or in the same room as equipment that could potentially emit sparks.

• Altitude not exceeding 1 828 m (6 000 feet) above sea level

• An ambient temperature of 4 °C to 35 °C (39.2 °F to 95 °F)The rate of temperature change must not exceed 2 °C (3.6 °F) per hour. Ambient temperature fluctuationsexceeding these limits will result in higher signal drift and wander of the baseline signal of the detector.

• Relative humidity from 20% to 85%, non-condensing with these exceptions:

• SPD-M20A: 45% to 85%, non-condensing

• ExionLCTM AC and SIL-20A/AC/HTA/HTC: 80% or less, non-condensing

Note: If ambient temperature is 30°C or greater, then relative humidity must be less than or equal to 70%.

Note: Do not place the system adjacent to heaters or cooling ducts, or in direct sunlight.

BioSafety RequirementsReturn to checklist.

The site must not be designated BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4). SCIEX does not install,service, or repair SCIEX systems in areas designated BSL-3 or BSL-4.

Computer and Network RequirementsReturn to checklist.

The computer is factory configured for the TCP/IP protocol. The product includes a fast Ethernet adapter(10/100baseT) with an RJ45 connector and one 2 m (6 ft) Ethernet cable.

• Obtain a network name for the computer.


Site Requirements

• Make sure that the default IP addresses for the modules are acceptable.

Default IP AddressModule

ExionLC System

192.168.200.99ExionLC Controller or ExionLC CBM-Lite

192.168.200.98ExionLC PDA Detector

192.168.200.100Computer

255.255.0Subnet mask

Prominence LC-20 HPLC System

192.168.200.99CBM-20A/CBM-20A Lite

192.168.200.98PDA

192.168.200.100Computer

255.255.0Subnet mask

1200/1260/1290 Series HPLC Systems

10.10.1.10System

10.10.1.11DAD

255.255.255.0Subnet mask

• (Optional) If the HPLC system computer will be connected to a LAN, make sure that an active, tested LANconnection is in place before the scheduled installation date.

Note: The SCIEX FSE cannot configure the system to access a specific network.

• If the LAN connector is more than 2 m (6 ft) from the HPLC System, supply a Category 5 RJ45 Ethernet cableof the required length.

• If required, for VWD detectors and other unsupported detectors, supply an ADC connection.

• (CTC PAL autosampler) Configure serial ports consecutively from COM1 to COM4 to allow communication withthe CTC PAL autosampler.


Site Requirements

Customer Supplied Solutions and EquipmentReturn to checklist.

WARNING! Toxic Chemical Hazard. Refer to chemical product Safety Data Sheetsand follow all safety guidelines when handling, storing, and disposing of chemicals.

General Equipment and Consumables• Powder-free gloves (neoprene or nitrile recommended

• Lint-free wipes

• Safety glasses

• Laboratory coat

• Three sizes of micropipettors and tips:

• 1 µL to 10 µL

• 10 µL to 100 µL

• 100 µL to 1000 µL

Glassware• 10 autosampler vials and caps

• 20 mL scintillation vials for dilutions

• Solvent bottles (4)

• Waste containers, 4 L

Consumables and Reagents• Glassware washing solutions

• MS-grade methanol (4 L)

• HPLC-grade DI water (4 L)

Additional supplies and consumables might be necessary for routine operation of the HPLC system. Before thesystem is installed, contact the SCIEX sales representative to order these supplies.


Site Requirements

CategoryDescription

Pollution Degree 2Equipment pollution degree

Note: Environments with a Pollution Degree 2 rating include laboratories and sales and commercial areas.

For more information, refer to the International Electrotechnical Commission standards IEC 61010-1 and IEC 60364.


BEquipment Safety Categories

DateReason for ChangeRevision

May 2014New template and incorporation of SIL-30ACMP content. Replaces 4442468D.A

June 2014Correction to supported instruments.B

May 2015Updated for ExionLCTM systems.C


Revision History

hplc system site planning guide - sciex

Documents