huacaya bvdv in alpaca: abortion and canada 17,000 ... · pdf filebvdv in alpaca: abortion and...
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![Page 1: Huacaya BVDV in Alpaca: Abortion and Canada 17,000 ... · PDF fileBVDV in Alpaca: Abortion and Persistent Infection ... Diarrhea 2 . 5 m o n 9 m o n 3 m o n Alberta 3 ... NCP-BVDV](https://reader033.vdocument.in/reader033/viewer/2022051203/5a9fe7187f8b9a7f178d6e87/html5/thumbnails/1.jpg)
BVDV in Alpaca: Abortion and
Persistent Infection
Susy Carman 1Murray Hazlett 1
Josepha Delay 1Nancy Carr 2Mohit Baxi 3
Dirk Deregt 3
Huacaya
Canada 17,000Ontario 1,300
Tested 101 alpaca28 herds
5 BVDV infected3 BVDV PI2 PI studied
Suri
University of Guelph 1Elginburg,ON 2
Canadian Food Inspection Agency 3
Denver, 2006
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Alberta 1 Alberta 2 Ontario 4
Ontario 552 alpaca
9 sick, 1 diedstress breaks
fleece
Aborted Fetus5.5 mon
IHC lu,ki,ht + BVDVVI + NCP-BVDV skin only
17/20 -Type 1 NADL - 1:3072Type 2 NVSL 125c - 1:394
2 early pregnancy
losses 75-80d gest
13 cria born1 Low birth weight
BVDV PIDiarrhea
2.5 mon
9 m
on3 mon
Alberta 3
Gabriel
Sickly criaLeBron born3,500 km
LeBron died 8 monPneumonia, Diarrhea
5 mon
abort/still/neo/BVDVAb
Ontario 6Aborted Fetus
7 mon
6 mon
PregnantFemale
Herd History PI Cria1
3 mon
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East
Gabriel’shuacaya farm
52 alpaca
Dam & cria barn facing south
Fleece storage
Courtesy Nancy Carr
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Herd History PI Cria 21,500 km
Ontario 764 alpaca
New Brunswick 997 alpaca
2 abortions
9/10 - Type 1 NADL - 1:384Type 2 NVSL 125c - 1:96
21 tested1 Low birth weight cria
PI BVDV
PregnantFemale
4 mons ges
Lilahuacaya
4monthsPneumonia, Diarrhea
Ontario 833 alpaca
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Lila - PIGabriel - PI● Exposed before120d gestation● Exposed about 65d gestation
(Dam Ab Type 1, 1:768)
● NCP-BVDV iso 75d buffy coat; 75d,104d,120d serum; 120d tissues
● NCP-BVDV iso from placenta;3d, 26d, 46d buffy coat; 46d tissues
● RT-PCR - BVDV Type 1, - whole blood-/+plasma, -/+serum, + buffy coat
● RT-PCR - BVDV Type 1,-/+/- serum 120d
● Ag ELISA 46d + serum, + ear notch ● Ag ELISA 120d +serum
● Serum virus titer 105.0 CCID50 /mL● Serum virus titer 104.0 CCID50 /mL
● Serum Ab titer 120d - neg Type 1, 2● Serum Ab titer 46d - neg Type 1,2
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ItalyPader
V 60
Gifhorn
T1802
V2536BDV
SCPGi6
CD87
125c
BVDV24812
SD-1
C86
NADLSinger
OslossCP7
Hastings
BVDV1b
Alpaca-24913
BVDV1a
H138
Giraffe
Eystrup
CSFV
100
100
100
97100
100
9991
57
100
8988
7590
6262
100
Gabriel Type1b - 4 NA / 2AA diff
Courtesy of Dr. Mohit Baxi
PI Cria 1 BVDV E2 gene
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Lila AxillaGabriel Ear
Hair follicle
Skin
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Gabriel Lila
Brain
Retina
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Gabriel
Thymus
Lila
BoneMarrow
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Gabriel Lila
Compartment 3Stomach
Colon
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Gabriel Lila
Kidney
Chorioallantoic membraneplacenta
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Need to update recommendations for testing before next alpaca birthing season
(1 vs 16 or 39 BVDV PI)
• Test/specimen to identify BVDV PI cria?
• VI +/+ buffy coat • RT-PCR + buffy coat• IHC + placenta• IHC +/+ haired skin• Ag ELISA +/+ serum, + haired skin• Multiple tests on low birth weight cria?
• Vaccination? – assessment of herd exposure
• Cerebellar Hypoplasia? • Mucosal Disease?
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Thyroidgland
LilaGabriel
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Alberta 1 Alberta 2 Ontario 4
Ontario 552 alpaca
9 sick, 1 deadstress breaks
fleece
Aborted Fetus5.5 mon
IHC lu,ki,ht + BVDVVI + NCP-BVDV skin only
14/17 -Type 1 NADL - 1:3072Type 2 NVSL 125c - 1:394
2 early pregnancy
losses 75-80d gest
13 cria born1 Low birth weight
PI BVDVDiarrhea
2.5 mon
9 m
on3 mon
Alberta 3
Gabriel
Sickly criaLeBron born3,500 km
LeBron died 8 monPneumonia, Diarrhea
5 mon
abort/still/neo/BVDVAb
Ontario 6Aborted Fetus
7 mon
6 mon
Female
Herd History PI Cria1
3 mon