MISEP Chem 512 - JacobsPOGIL: Energy of ATP hydrolysis

ATP can react with water (a hydrolysis reaction) to produce ADP and a phosphate groupComplete the figure below by filling in the boxes to illustrate this reaction. Be sure toaccount for conservation of all atoms.

-O—

OH OH

hydrolysis reaction

NH,

\d«&» OH OH

ADP

/V7<

- 0

phosphate

Concept-building questions:

1. What bond(s) is/are broken during the ATP hydrolysis reaction?

2. Is energy(absorbecPor released during the bond-breaking step(s)? Explain why."

D C J**Ms X^r^-)cL^ fiWStST**J<<*r*

f t ) v3. What bond(s) is/are formed during the ATP hydrolysis reaction?

WvJfvxK .~r -A A0 o T 6- b4. Is energy absorbed ofCfeleaseJ1 during the bond forming step(s)? Explain why.

^*-

?ondsj?n either the reactants^^'the^products'of the hydrolysis reactiondrawn above that are either particularly strong/stable or particularly weak/unstable?Explain.

V

\̂NS-

^ ^/WJ^ML-

H^JC^ *

6. Describe how yettr answers to the previous questions (#1-5) can be used to explainwhy ATP hydrolysis is an exothermic reaction overall.

SynthesisKsmd Application questions:

1. Draw a potential energy diagram representing this reaction. If this reaction happenedin solution in a test tube, would you expect the test tube to feel warm or cool? Why?Link together the macroscopic, molecular and symbolic levels of the ATP hydrolysisreaction in your answer.

8. Although ATP hydrolysis is energetically favorable (exothermic)rit occurs veryslowly under uncatalyzed conditions. How is this observation reflected in the potentialenergy diagram for the hydrolysis reaction you drew in question 7? Redraw yourdiagram if necessary to reflect this new information. , j^

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