hylasome eg10 - lipo chemicals eg10_.… · • hylasome® is the tradename of hylan b gel...
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Hylasome® EG10 Excellent Moisturizing Through the Delivery of Water
Hylasome® is a registered trademark of Genzyme Corpora�on. US Patents 5,143,724; 5,246,698; 5,399,351; other patent pending
• Hylasome® is the tradename of Hylan B gel (cross-‐linked hyaluronic acid) used in topical applica�ons.
• HylaMed Research, Inc. has the exclusive, global license for Hylasome technology for use in the topical cosme�c and skin care field.
• Lipo Chemicals is the exclusive worldwide distributor for HylaMed Research.
• Medical Products containing Hylan B gel:
– Synvisc® : treatment for osteoarthri�s; injec�on of Synvisc (Hylan B gel/Hylan A HA mixture) into the knee relieves the pain of osteoarthri�s.
– Hylaform®: so� �ssue augmenta�on-‐ correc�on of facial wrinkles and scars by intradermal injec�on of Hylaform (composed of Hylan B gel)
• INCI name: Sodium Hyaluronate Crosspolymer
• CAS No.: 380600-‐88-‐4
• EINECS No.: Polymer, Exempt
• Consists of an aqueous gel of crosslinked hyaluronic acid.
• Polymer content: 6% by weight of polymer plus bound water
Chemical Structure
Sulfonyl-‐bis-‐ethyl crosslinks and hydroxyethyl sulfonyl ethyl pendant groups
O
O
HO
HH
H
OHO
COO -
O
OH
H
OH
H
H
NHH
OH
H
OCH3
O
HO
HH
H
OHHO
COO -
O
O
OH
H
OH
H
H
NHH
O
O
OCH3
O
O
HO
HH
H
OHHO
COO-
O
O H
H
OH
H
H
NHH
OH
H
OCH3
O
HO
HH
H
OHHO
COO-
O
O
OH
H
OH
H
H
NHH
O
O
OCH3
S OO
SO O
OH
Crosslinking
Delivery of Water • Unique non-‐equilibrium gel.
• Developed specifically in a non-‐equilibrium state with gel domains that are not completely swollen.
• Gel domains, which contain �ghtly bound water, are surrounded by free water.
• The gel forms a smooth film on the skin and con�nuously delivers the bound water.
Skin Moisturiza�on Study:
• Confocal Raman Spectroscopy
• Ex vivo – surgically removed skin (including stratum corneum, viable epidermis, and some dermis).
• Hylasome® EG10 vs. Hyaluronic Acid, both at 0.1% polymer weight in D2O
• Performed at Rutgers University
Experimental Procedure • 5 µL D2O-‐based test material applied to 2x2 cm2
surgically removed skin.
• Held at 55% rela�ve humidity (D2O) and room temperature for 24 hours.
• Placed in milled brass sample holder and covered with glass cover slip.
• D2O content in the skin measured by confocal Raman spectroscopy in the x and z axes.
Confocal sampling • skin sec�ons parallel to surface • placed in a brass block (SC up) • sealed with cover slip
Cover glass
Brass block
Skin sample
A well is milled into the brass block
785 nm in Raman out
100 X objec�ve (oil immersion) Raman out
Measure along Z-‐line
785 nm in
Confocal Raman Spectoscopy
Raman Spectra of Materials
0
5000
10000
15000
20000
2100 2200 2300 2400 2500 2600 2700 2800 2900 3000 3100
Hyaluronate prep Hylasome prep Ethylhexyl glycerin Pentylene glycol
Raman cou
nts
D2O stretching mode
CH2 CH3 stretching region
Raman Shi� (cm-‐1)
0
500
1000
1500
2000
2500
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500
CH2, CH3 scissoring region
CH2 twis�ng
Typical Raman spectrum of surgically removed human skin stratum corneum exposed to 100% Rela�ve humidity D2O
Integrated band area ra�o of D2O / Phe is used to quan�fy the rela�ve concentra�on of D2O in skin. Skin protein component phenylalanine (Phe), band area is used as an “internal standard” to correct for sca�ering losses with depth. The ra�o of the D2O peak to the Phe peak indicates the moisture content at the measurement site.
Wavenumber (cm-‐1)
1000 1500 2000 2500 3000
0
2000
4000
6000
8000
10000
12000
Raman intensity
700 800 900 1000 1100 1200 -‐600
-‐400
-‐200
0
200
400
600
Raman intensity
D2O stretching band
Phe ring breathing mode
Phe ring breathing mode
This skin sec�on had Hylasome EG10 applied to the top of the SC.
Wavenumber (cm-‐1)
Applica�on in 55% Rela�ve Humidity D2O environment D2O / Phe band area ra�o
400 Across skin, µm
30
Hylasome® EG10 En�re sample area Mean ra�o: 693
500
600
700
800
900
1000
1100
1200
1300
Depth into skin, µm
0 10 20 30
0
5
10
15
20
25
Depth into skin, µm
Across skin, µm
50
100
150
200
250
300
0 5 10 15 20
0
5
10
15
20
25
30
Hyaluronic Acid En�re sample area Mean ra�o: 111
Skin appears to dry over �me while exposed to Raman laser. Spectra acquired le� to right.
A�er 24 hour incuba�on at 55% Rela�ve Humidity in a D2O environment, the results show that the sec�on treated with the Hylasome® prepara�on contains more D2O in the skin and in the stratum corneum than the sec�on treated with the 0.1% HA formula�on. Higher ra�o numbers indicate higher D2O concentra�on.
55% Rela�ve Humidity D2O environment D2O / Phe band area ra�o for stratum corneum region using same color scale
• A�er 24 hrs at 55% Rela�ve Humidity:
– Hylasome® EG10 treated skin contained 6x more moisture in the total sample and 5x more moisture in the stratum corneum than hyaluronic acid treated skin.
Conclusion
Free Radical Scavenger • Hyaluronic Acid (HA):
– Natural scavenger of free radicals (Greenwald & Moy)
– Protects against free radical-‐induced cell damage, molecular weight dependent (Pres� & Sco� 1994)
– Prevents free radical damage to healing �ssue (Foschi, et al 1990)
– Inhibits lipid peroxida�on (Alber�ni, et al 2000) • Hylasome® gel:
– Inhibi�on of free radical produc�on; free radical consump�on (Larsen et al 1992)
– Accelerates healing of full thickness wounds in rats (SBIR grant final report, 1987, Biomatrix, Inc.)
An�oxidant Ac�vity: Free Radical Scavenging by Hylasome® [xanthine oxidase/hypoxanthine] Measured in vitro
Perc
ent
Redu
c�on
of F
ree
Radi
cals
Hylasome® is : • Nonirrita�ng
• Nonsensi�zing • Noncytotoxic • Nonphototoxic • Nonhemoly�c
• Nonpyrogenic • Nonmutagenic
• Passes HRIPT and Epi-‐Ocular Tests.
Safety
• Skin Care Creams and Lo�ons
• Skin Care Gels
• Skin Care Treatment Products
• Facial Masks
• Liquid Founda�on Make ups
• Leave-‐in hair condi�oners and styling aids (without ca�onic compounds)
• Recommended Use Level: 1 to 3%
Product Formula�on Types
Benefits: • Five �mes the water binding capacity of hyaluronic acid
• Free radical scavenger • Smooth elegant skin feel
• Slow delivery of water – Long Term Moisturiza�on Claim
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