i. personal history and professional...

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Curriculum Vitae: Huimin Zhao

University of Illinois at Urbana-Champaign (UIUC)

215 Roger Adams Laboratory, Box C-3

600 South Mathews Avenue

Urbana, Illinois 61801-3602

Voice: 217-333-2631, Fax: 217-333-5052

E-mail: [email protected], http://scs.illinois.edu/~zhaogrp/

I. PERSONAL HISTORY AND PROFESSIONAL EXPERIENCE

A. Educational Background

University of Science and Technology of China (USTC), B.S., Biology, June 1992

California Institute of Technology, Ph.D., Chemistry, June 1998

B. List of Academic Positions since Final Degree

Steven L. Miller Chair of Chemical and Biomolecular Engineering, UIUC, 2016-date

Qiushi Chair Visiting Professor, Zhejiang University, 2015-date

Theme Leader, Institute for Genomic Biology, UIUC, 2014-date

Visiting Principal Investigator, Agency for Science, Technology and Research (A*STAR),

Singapore, 2012-2018

Visiting Professor, Nanyang Technological University, 2011-2018

Centennial Endowed Chair of Chemical and Biomolecular Engineering, UIUC, 2008-2016

Professor, Chemical and Biomolecular Engineering, UIUC, August 2008-date

Associate Professor, Chemical and Biomolecular Engineering, UIUC, August 2006-2008

Assistant Professor, Chemical and Biomolecular Engineering, UIUC, July 2000-August 2006

Faculty, Institute for Genomic Biology, UIUC, March 2004-date

Affiliate, Chemistry, UIUC, January 2004-date

Affiliate, Center for Biophysics and Computational Biology, UIUC, December 2000-date

Affiliate, Bioengineering, UIUC, December 2000-date

Affiliate, Biotechnology Center, UIUC, December 2000-date

Affiliate, Biochemistry, UIUC, August 2007-date

C. Other Professional Employment

Project Leader, Industrial Biotechnology, The Dow Chemical Company, San Diego, CA, April

1998 - May 2000

D. Honors, Recognitions, and Outstanding Achievements

ECI Enzyme Engineering Award, 2019

Honorary Guest Professor, Nankai University, 2019

Program Chair, ECI Enzyme Engineering Conference XXV, 2019

Institute of Advanced Studies Lecture, Hong Kong University of Science and Technology, 2018

Marvin Johnson Award, American Chemical Society (ACS) Division of Biochemical

Technology, 2018

Biotechnology Progress Award for Excellence in Biological Engineering Publications, 2017

Honorary Guest Professor, Jiangnan University, 2017

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Charles Thom Award, Society for Industrial Microbiology and Biotechnology, 2016

Steven L. Miller Chair of Chemical and Biomolecular Engineering, UIUC, 2016-date

Qiushi Chair Visiting Professor, Zhejiang University, 2015

Elmer Gaden Award, John Wiley & Sons and American Chemical Society Division of Biochemical

Technology, 2014

Program Chair, ECI Biochemical Engineering Conference XVIV, 2013

Visiting Principal Investigator, Agency for Science, Technology and Research (A*STAR),

Singapore, 2012-date

John Simon Guggenheim Fellowship, 2012

Session Chair, NAE’s Indo-American Frontiers of Engineering Symposium, 2012

National Academies Keck Futures Initiative Award, 2010

Merck Lecture, Department of Chemical Engineering, University of Virginia, 2010

Fellow of the American Association for the Advancement of Science, 2009

AIChE Food, Pharmaceutical, and Bioengineering Division Plenary Award Lecturer,

Biochemical Engineering (Area 15c), 2009

Invited Participant of 7th Annual National Academies Keck Futures Initiative (NAKFI)

Conference, 2009

Invited Participant of 15th U.S. Frontiers of Engineering Symposium, National Academy of

Engineering, 2009

Fellow of the American Institute for Medical and Biological Engineering (AIMBE), 2008

Centennial Endowed Chair of Chemical and Biomolecular Engineering, UIUC, 2008-2016

Engineering Council Award for Excellence in Advising, UIUC College of Engineering, 2008

Young Investigator Award, American Chemical Society (ACS) Division of Biochemical

Technology, 2008

University Scholar, UIUC, 2007

Outstanding Overseas Young Chinese Scholars Award, Chinese National Science Foundation,

2007

Helen Corley Petit Scholar, UIUC College of Liberal Arts and Sciences, 2006

DuPont Young Professor Award, 2005

Xerox Award for Faculty Research, UIUC College of Engineering, 2005

W. H. Peterson Award for Best Poster Presentation, ACS Division of Biochemical Technology

(to R. Woodyer), 2005

Beckman Fellow, UIUC Center for Advanced Study, 2005

NSF CAREER Award, 2004

Excellence in Teaching Award, UIUC School of Chemical Sciences, 2004

Collins Scholar, UIUC College of Engineering, 2001

Dow Chemical Company Special Recognition Award, 1999 and 2000

E. Invited Lectures and Invited Conference Presentations

1. “Directed Evolution of Biocatalysts.” The Dow Chemical Company, Midland, MI, October

20, 1997.

2. “Directed Molecular Evolution.” Shanghai Institute of Biochemistry, Chinese Academy of

Science, Shanghai, China, May 20, 2000.

3. “Directed Molecular Evolution.” Institute of Life Sciences, Fudan University, Shanghai,

China, May 22, 2000.

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4. “A Dehalogenase Based Process for Epichlorohydrin Production.” IBC Sixth Annual

World Congress on Enzyme Technologies, San Diego, CA, February 28, 2001.

5. “Directed Evolution of Enzymes and Pathways for Bioprocess Development.” Bristol-

Myers Squibb, New Brunswick, NJ, March 12, 2001.

6. “Directed Molecular Evolution: Technology and Applications.” Department of

Pharmacology, University of Florida, Gainesville, FL, February 5, 2002.

7. “Directed Molecular Evolution.” Cabot Corporation, Boston, February 26, 2002.

8. “Outrunning Nature: in vitro Evolution of Enzymes.” Biotechnology Research Institute,

Canadian National Research Council, Montreal, Canada, March 28, 2002.

9. “Directed Evolution of Enzymes and Industrial Biocatalysis.” Green Chemistry Workshop,

University of Illinois, Urbana, IL, April 19, 2002.

10. “Directed Evolution of Superior Biocatalysts.” Maxygen, Redwood City, CA, May 6, 2002.

11. “Directed Evolution and Biocatalysis.” Department of Energy (DOE) Workshop on

Catalysis, Gaithersburg, Maryland, May 14, 2002.

12. “Directed Evolution of Enzymes, Pathways and Genomes.” U.S. Army Research Office

(ARO) Workshop on Bio-Fuel Cells, Washington, DC, July 1, 2002.

13. “Directed Evolution of Human Manganese Superoxide Dismutase.” ACS Annual Meeting,

Boston, MA, August 21, 2002.

14. “Biomolecular Engineering in the Post-genomic Era.” Molecular and Chemical Biology

Symposium, University of Illinois, Urbana, IL, January 17, 2003.

15. “Directed Evolution of Enzymes and Multi-enzyme Complexes.” Strategic Research

Institute’s First Annual Meeting on “Enzymes and Biocatalysis for Drug Discovery and

Development.” San Diego, CA, January 30, 2003.

16. “Biosynthesis of Thermally Stable Energetic Compounds through Rational Design and

Directed Evolution.” Office of Naval Research’s Coolfont V meeting, Coolfont, West

Virginia, April 13, 2003.

17. “Biocatalysts Engineering Through Rational Design and Directed Evolution.” Schering-

Plough, Union, NJ, August 5, 2003.

18. “Biocatalysts Engineering Through Rational Design and Directed Evolution.” Cargill,

Minneapolis, MN, September 9, 2003.

19. “Biomolecular Engineering in the Post-Genomic Era.” Department of Food Science and

Human Nutrition, University of Illinois, Urbana, IL, September 25, 2003.

20. “Protein Engineering of Phosphate Dehydrogenase for the Development of a Novel

NAD(P)H Regeneration System.” ACS Annual Meeting, Anaheim, CA, March 28, 2004.

21. “Biomolecular Engineering for Biomedical Applications.” Department of Biomedical

Engineering, University of Irvine, Irvine, CA, April 1, 2004.

22. “Protein Engineering of Nanomachines.” Beckman Institute, University of Illinois, Urbana,

IL, April 14, 2004.

23. “Directed Evolution of Human Estrogen Receptors for Fun and Profits.” Symposium on the

Evolution of Biomolecular Structure, Michigan State University, East Lansing, MI, June 4,

2004.

24. “Directed Molecular Evolution for Fun and Profit.” Monsanto, St. Louis, MO, July 7, 2004.

25. “Biocatalyst Development through Rational Design and Directed Evolution.” Roquette,

Lestrem, France, November 15, 2004.

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26. “Protein Stabilization by Directed Evolution.” DARPA/HHS Protein Preservation

Workshop, Fairfax, VA, January 12, 2005.

27. “Biocatalyst and Bioprocess Development via Biomolecular Engineering.” Kraft,

Glenview, IL, January 31, 2005.

28. “Directed Evolution: Technology and Applications.” Dow AgroSciences, Indianapolis, IN,

February 7, 2005.

29. “Biomolecular Engineering for Fun and Profit.” Department of Chemical and Biological

Engineering, University of Wisconsin, Madison, WI, February 8, 2005.

30. “Directed Molecular Evolution for Fun and Profit.” Argonne National Laboratories,

Argonne, IL, February 17, 2005.

31. “Biomolecular Engineering of Genetic Switches and Biological Catalysts.” Division of

Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA,

February 24, 2005.

32. “Biomolecular Engineering of Genetic Switches and Biological Catalysts.” Department of

Chemical and Environmental Engineering, University of California, Riverside, CA,

February 25, 2005.

33. “Development of a Novel Enzymatic NAD(P)H Regeneration System.” The Tenth Institute

of Biological Engineering, University of Georgia, Athens, GA, March 4-6, 2005.

34. “Biomolecular Engineering for Biocatalyst and Bioprocess Development.” Symposium on

Biocatalysis, ACS Annual Meeting, San Diego, CA, March 13, 2005.


Chemical and Biological Engineering, Northwestern University, Evanston, IL, April 14,

2005.

36. “Development of a Novel Enzymatic NAD(P)H Regeneration System for Industrial

Biocatalysis.” Biochemical Engineering XIV, Harrison Hot Springs, BC, Canada, July 14,

2005.

37. “Directed Evolution of Proteins and Pathways.” U.S. Government’s Science and Technical

Expert Partnership (STEP) Program Workshop on Synthetic Biology, McLean, VA, July

27, 2005.

38. “Biomolecular Engineering for Fun and Profit.” Department of Chemical and Biomolecular

Engineering, University of Illinois, Urbana, IL, September 13, 2005.

39. “Directed Evolution: Technology and Applications.” Air Force Office of Science and

Research Cell-Like Entity (CLE) workshop, Fairborn, OH, September 14, 2005.


Biocatalysis.” Biotechnology Research and Development Consortium (BRDC) workshop,

Chicago, IL, September 15, 2005.

41. “Biomolecular Engineering for Fun and Profit.” Department of Chemical Engineering,

University of Massachusetts, Amherst, MA, September 22, 2005.


Biocatalysis.” ECI Enzyme Engineering XVIII, Gyeong-Ju, Korea, October 13, 2005.

43. “Biocatalyst and Bioprocess Development via Biomolecular Engineering.” International

Symposium on Biocatalysis and Bioprocess Engineering II, Shanghai, China, October 17,

2005 (keynote lecture).


Tsinghua University, Beijing, China, October 18, 2005.

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45. “Biomolecular Engineering for Fun and Profit.” Institute of Biophysics, Chinese Academy

of Science, China, October 19, 2005.

46. “Biomolecular Engineering for Fun and Profit.” College of Life Sciences, University of

Science and Technology of China, Hefei, Anhui, China, October 20, 2005.

47. “Biomolecular Engineering for Fun and Profit.” Department of Biochemistry, Nanjing

University, Nanjing, China, October 21, 2005.


Purdue University, West Lafayette, IN, October 25, 2005.


University of California, Los Angeles, CA, January 13, 2006.

50. “Biomoelcular Engineering of Proteins and Pathways.” School of Molecular and Cell

Biology, University of Illinois, Urbana, IL, January 21, 2006.

51. “Biomoelcular Engineering of Proteins and Pathways.” Department of Chemical

Engineering, University of Delaware, Newark, DE, February 10, 2006.


Chemistry, Emory University, Atlanta, GA, February 13, 2006.

53. “Biomoelcular Engineering for Fun and Profit.” Department of Chemical Engineering,

University of Southern California, Los Angeles, CA, February 23, 2006.

54. “Bioinformatics Tools for Biomolecular Engineering.” The First UIUC Bioinformatics

Summit, University of Illinois, Urbana, IL, March 9, 2006.

55. “Biomoelcular Engineering for Fun and Profit.” Department of Chemical Engineering,

Rensselaer Polytechnic Institute, Troy, NY, March 27, 2006.

56. “Biomass Conversion through Biomolecular Engineering.” Symposium on Sustainable

Bioenergy: Focus on the Future of Biofuels and Chemicals, Urbana, IL, April 13-14, 2006.

57. “Biocatalyst Development through Biomolecular Engineering.” Society for Industrial

Microbiology Annual Meeting, Baltimore, MD, August 2, 2006.

58. “Directed Evolution of Genetic Switches and Circuits.” Nano-Bio Group, Urbana, IL

November 3, 2006.

59. “Biomolecular Engineering for Fun and Profit.” Department of Chemical Engineering and

Materials Science, University of Minnesota, Twin Cities, MN, November 21, 2006.


Physics, University of Illinois, Urbana, IL, March 9, 2007.

61. “Directed Evolution of Gene Switches and Enzyme Biocatalysts.” Northern Illinois

University, April 27, 2007.

62. “Directed Evolution of Gene Switches and Enzyme Biocatalysts.” Shanghai Institute of

Plant Physiology, Chinese Academy of Science, Shanghai, China, May 11, 2007.

63. “Biomolecular Engineering for Fun and Profit.” School of Chemical Engineering and

Technology, Tianjin University, May 15, 2007.

64. “Biomolecular Engineering for Fun and Profit.” School of Biochemical Engineering, East

China University of Science and Technology, May 22, 2007.

65. “Industrial Biotechnology: Surfing the Third Wave of Biotechnology.” Department of

Chemical Engineering and Materials Science, University of Minnesota, Twin Cities, MN,

June 7, 2007.

66. “Biosynthesis of Xylitol from Renewable Biomass.” ECI Biochemical Engineering XV,

Quebec City, PQ, Canada, July 16, 2007.

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67. “Evolution in Reverse: Engineering a Xylose-Specific Xylose Reductase.” Society for

Industrial Microbiology Annual Meeting, Denver, CO, August 1, 2007.

68. “Biomolecular Engineering for Industrial Biotechnology and Bioenergy.” Biotechnology

Institute, University of Minnesota, Twin Cities, MN, August 23, 2007.

69. “Discovery, Characterization, and Engineering of Phosphonic Acids.” Department of

Chemistry, Vanderbilt University, Nashville, TN, September 10, 2007.

70. “Surfing the Third Wave of Biotechnology.” Department of Chemical Engineering,

University of Colorado, Denver, CO, October 30, 2007.

71. “Surfing the Third Wave of Biotechnology.” Department of Chemical Engineering,

Princeton University, Princeton, NJ, December 12, 2007.

72. “Biomolecular Engineering for Fun and Profit.” Department of Biomedical and Chemical

Engineering, Syracuse University, Syracuse, NY, February 29, 2008.

73. “Synthetic Biology for Fun and Profit.” 2nd Annual IGB Fellows Symposium, University of

Illinois, Urbana, IL, April 4, 2008.

74. “Development of New Biocatalysts for Pharmaceutical Applications.” Pfizer Global R &

D, Groton, CT, April 7, 2008.

75. “Microbial Synthesis of Phloroglucinol and Xylitol.” Fifth World Congress on Industrial

Biotechnology and Bioprocessing, Chicago, IL, April 30, 2008.

76. “Production of fine chemical phloroglucinol and antimalarial drug FR900098 in E. coli.”

ECI Natural Products II, Whistler, Canada, June 24, 2008.

77. “Microbial Synthesis of Phloroglucinol and Xylitol.” The 2008 Korean Society of

Microbiology and Biotechnology (KMB) International Symposium and Annual Meeting,

Seoul, Korea, June 26, 2008.

78. “Industrial Biotechnology: Surfing the Third Wave of Biotechnology.” Kongkuk

University, Seoul, Korea, June 27, 2008.

79. “Enzymatic and Microbial Synthesis of Xylitol from Renewable Biomass.” Gordon

Research Conference on Biocatalysis, Smithfield, RI, July 6-11, 2008.

80. “Metalloenzymes: Mechanisms, Structures, and Applications.” NSF CENTC Summer

School, University of Washington, Seattle, WA, July 24, 2008.

81. “Discovery, Characterization, and Engineering of Novel N-Oxygenases.” Society for

Industrial Microbiology Annual Meeting, San Diego, CA, August 12, 2008.

82. “Directed Evolution for Fun and Profit.” American Chemical Society Annual Meeting,

Philadelphia, PA, August 20, 2008.

83. “New Tools for Pathway Engineering.” Gevo, Inc., Denver, CO, August 25, 2008.

84. “Development of New Tools for Gene Therapy.” Cystic Fibrosis Focus Group, University

of Illinois, Urbana, September 11, 2008.

85. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” University of

California, Berkeley, CA, September 29, 2008.

86. “Microbial Synthesis of Phloroglucinol and Xylitol.” The Thirteenth International

Biotechnology Symposium and Exhibition, Dalian, China, October 15, 2008.

87. “Industrial Biotechnology: Surfing the Third Wave of Biotechnology.” Jiangnan

University, Wuxi, China, October 17, 2008.

88. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Institute of Process

Engineering, Chinese Academy of Sciences, Beijing, China, October 20, 2008.

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89. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Beijing University

of Chemical Technology, Beijing, China, October 21, 2008.

90. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Genencor Shanghai

R&D Center, Shanghai, China, October 23, 2008.

91. “Harnessing the Power of Synthetic Biology and Chemical Biology.” Shanghai Jiaotong

University, Shanghai, China, October 24, 2008.

92. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Novozymes, Davis,

CA, November 3, 2008.

93. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Rice University,

Houston, TX, January 8, 2009.

94. “New Tools for Pathway Engineering and Microbial Synthesis of Antimalarial Drug

FR900098.” International Conference on Biomolecular Engineering II, Santa Barbara, CA,

January 20, 2009.

95. “Engineering a Recombinant Yeast Strain that Efficiently Utilizes C5/C6 Sugars.” EBI

Program Leaders Symposium, January 31, 2009.

96. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Department of

Statistics, University of Illinois, Urbana, IL, March 5, 2009.

97. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Bioenergy

International, Woburn, MA, March 11, 2009.

98. “Harnessing the Power of Synthetic Biology to Address Challenges on Health Care and

Energy.” University of Massachusetts, Amherst, MA, March 12, 2009.

99. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” SUNY at Buffalo,

Buffalo, NY, April 8, 2009.

100. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Institute of

Chemical Engineering and Sciences, Singapore, May 27, 2009.

101. “Biocatalysis for Bioenergy.” Workshop on Catalysis for Sustainable Energy, National

University of Singapore, Singapore, May 28, 2009.

102. “Engineering of a C5/C6 Utilizing S. cerevisiae strain for Biofuel Production.” Energy

Biosciences Institute Annual Retreat, Urbana, IL, June 19, 2009.

103. “Harnessing the Power of Synthetic Biology and Chemical Biology.” University of

Cambridge, London, England, June 26, 2009.

104. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Max-Planck-Institut

für Kohlenforschung, Mülheim, Germany, June 29, 2009.

105. “Discovery and Engineering of Novel Enzymes and Pathways using DNA Assembler.”

Society for Industrial Microbiology Annual Meeting, Toronto, Canada, July 27, 2009.

106. “Engineering of a C5/C6 Utilizing S. cerevisiae strain for Biofuel Production.” Society for

Industrial Microbiology Annual Meeting, Toronto, Canada, July 28, 2009.

107. “Towards Cost-effective Production of an Antimalarial Drug and Butanol.” Foundations of

Systems Biology in Engineering (FOSBE), Denver, CO, August 7, 2009.

108. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” National Renewable

Energy Laboratory, Golden, CO, August 11, 2009.

109. “Synthetic Biology for Fun and Profit.” Keynote lecture in the Chinese American Chemical

Society’s Annual Dinner Party, Washington, DC, August 17, 2009.

110. “Microbial Synthesis of Drugs and Fuels via Synthetic Biology.” Department of Chemical

Engineering, University of Texas, Austin, TX, October 13, 2009.

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111. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Department of

Bioengineering, University of Illinois, Urbana, IL, October 15, 2009.

112. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” AIChE FP&B

Division Plenary Award Lecture, Nashville, TN, November 9, 2009.

113. “Harnessing Synthetic Biology for Translational Biomedical Research.” Department of

Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, IL,

December 9, 2009.


Engineering, University of Virginia, Charlottesville, VA, February 18, 2010.


Engineering, Massachusetts Institute of Technology, Cambridge, MA, April 9, 2010.


Energy.” Department of Chemical Engineering, Pennsylvania State University, College

Station, PA, April 20, 2010.

117. “Harnessing Synthetic Biology for Translational Biomedical Research.” International

AIDS Vaccine Initiative (IAVI)'s Frontiers in AIDS Vaccine Development Seminar Series,

New York City, NY, July 14, 2010.

118. “New Strategies to Overcome Glucose Repression in Mixed Sugar Fermentation in

Saccharomyces cerevisiae.” Society for Industrial Microbiology Annual Meeting, San

Francisco, CA, August 4, 2010.

119. “Engineering a Microbial Factory for Synthesis of Value-added Products.” Society for

Industrial Microbiology Annual Meeting, San Francisco, CA, August 5, 2010.

120. “Designing Microbial Factories for Synthesis of Fuels and Chemicals.” International

Conference on Biomass and Energy Technologies, Beijing, China, August 22, 2010

(keynote lecture).

121. “Microbial Synthesis of Drugs and Fuels via Synthetic Biology.” Tianjin Institute of

Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China, August 23, 2010.


Energy.” The First Annual Industrial Biotech Meeting, Xiamen, Guangzhou, China, August

24, 2010 (keynote lecture).

123. “Microbial Synthesis of Drugs and Fuels via Synthetic Biology.” Tsinghua University,

Beijing, China, August 25, 2010.

124. “Microbial Synthesis of Drugs and Fuels via Synthetic Biology.” Institute of Microbiology,

Chinese Academy of Sciences, Beijing, China, August 26, 2010.


Engineering, Washington University at St. Louis, MO, September 10, 2010.


Engineering, North Carolina State University, Raleigh, NC, October 4, 2010.

127. “East Meets West or West Meets East: A Personal Experience with Transnational Research

Collaboration.” Conference on East Asian Biosciences: Transnational Competition and

Collaboration, Urbana, October 8, 2010.

128. “Microbial Synthesis of Drugs and Fuels via Synthetic Biology.” School of Chemical

Engineering, East China University of Science and Technology, Shanghai, China, October

18, 2010.

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129. “Microbial Synthesis of Drugs and Fuels via Synthetic Biology.” Key Laboratory of

Synthetic Biology, Chinese Academy of Sciences’ Institute of Plant Physiology, Shanghai,

China, October 18, 2010.


Engineering, University of Houston, Houston, TX, October 29, 2010.

131. “Protein Engineering by Directed Evolution and Rational Design.” Chinese Academy of

Sciences and Max Planck Gesellschaft’s Exploratory Round Table Conference, Shanghai,


132. “Engineering a Microbial Factory for Synthesis of Value-added Products.” Pacifichem

2010, Honolulu, HI, December 18, 2010

133. “Engineering Cytochrome P450 Enzymes for Biocatalysis.” Pacifichem 2010, Honolulu,

HI, December 19, 2010

134. “Microbial Synthesis of Drugs and Fuels via Synthetic Biology.” Institute of Chemical

Engineering and Sciences, Singapore, January 10, 2011.

135. “Microbial Synthesis of Drugs and Fuels via Synthetic Biology.” International Conference

on “Big Ideas in Molecular Materials,” Singapore, January 11, 2011.


and Biomolecular Engineering, National University of Singapore, Singapore, January 12,

2011.


and Biomolecular Engineering, Nanyang Technical University, Singapore, January 13,

2011.


Engineering, University of Washington at Seattle, Seattle, WA, February 14, 2011.


Engineering, Arizona State University, Tempe, AZ, April 15, 2011.

140. “Synthetic Biology: Putting Synthesis into Biology.” School of Chemical Engineering,

Tianjin University, Tianjin, China, June 27, 2011.

141. “Synthetic Biology: Putting Synthesis into Biology.” Department of Chemical and

Biomolecular Engineering, Nanyang Technical University, Singapore, June 29, 2011.

142. “Microbial Synthesis of Sustainable Chemicals and Materials.” Institute of Chemical

Engineering and Sciences, Singapore, June 30, 2011.

143. “Synthetic Biology: Putting Synthesis into Biology.” School of Life Sciences, University of

Science and Technology of China, July 4, 2011.

144. “Engineering Cytochrome P450 Enzymes for Biocatalysis.” Society for Industrial

Microbiology Annual Meeting, New Orleans, LA, July 25, 2011.

145. “Discovery, Design, and Development of Novel Natural Products via Synthetic Biology.”

Society for Industrial Microbiology Annual Meeting, New Orleans, LA, July 26, 2011.

146. “Development of Novel Tools for Gene Therapy via Protein Engineering.” The 11th

Emerging Information and Technology Conference, Chicago, IL, July 28, 2011.

147. “Biocatalyst by Design: Challenges and Opportunities.” NSF Workshop on Catalysis,

Denver, CO, August 27, 2011.


Engineering, Iowa State University, Ames, IA, September 8, 2011.

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Engineering, Northwestern University, Evanston, IL, October 6, 2011.

150. “Design and Evolution of Biological Pathways and Networks.” Six-Academy’s

Symposium on Synthetic Biology, Shanghai, China, October 12, 2011.

151. “Synthetic Biology: Putting Synthesis into Biology.” First Annual Biomass Conversion

Symposium, Urbana, IL, Oct. 31, 2011.

152. “Engineering Microbial Factories for Sustainable Synthesis of Value-added Products.” 2nd

International Conference on Green and Sustainable Chemistry, Singapore, November 14,

2011 (opening plenary lecture).

153. “Engineering a Yeast Strain that Efficiently Utilizes C5/C6 Sugars.” EBI Annual Fall

Review, Berkeley, CA, November 17, 2011.

154. “Synthetic Biology: Putting Synthesis into Biology.” Brookhaven National Laboratory,

Upton, NY, Jan. 13, 2012.

155. “New Strategies to Engineer Yeast Strains for Biofuels Production.” BP Global

Technology Center, San Diego, CA, April 6, 2012.

156. “Engineering a Yeast Strain that Efficiently Utilizes C5/C6 Sugars.” EBI Annual Spring

Review, Berkeley, CA, April 10, 2012.

157. “Synthetic Biology: Putting Synthesis into Biology.” 2012 Hougen Symposium, Madison,

WA, May 1, 2012.

158. “Pathway Engineering via Synthetic Biology.” ECI Metabolic Engineering Conference IX,

Biarritz, France, June 5, 2012.

159. “Surfing the Third Wave of Biotechnology: Turning Trash into Cash.” Institute of

Catalysis, Spanish Council for Scientific Research (CSIC), Madrid, Spain, June 8, 2012.

160. “Synthetic Biology: Putting Synthesis into Biology.” Department of Chemistry, National

University of Singapore, July 25, 2012

161. “Synthetic Biology: Putting Synthesis into Biology.” China Pharmaceutical University,

Nanjing, China, July 31, 2012.

162. “Synthetic Biology: Putting Synthesis into Biology.” 2012 Sino-USA Chinese

Collaborative Workshop--Opportunities and Challenges in Synthetic Biology, Tianjin,

China, August 2, 2012.

163. “Pathway Engineering via Synthetic Biology.” Society for Industrial Microbiology and

Biotechnology Annual Meeting, Washington DC, August 13, 2012.

164. “Discovery of Novel Natural Products by Refactoring Cryptic Pathways.” Society for

Industrial Microbiology and Biotechnology Annual Meeting, Washington DC, August 14,

2012.

165. “Novel Tools for Genome Engineering.” Society for Industrial Microbiology and

Biotechnology Annual Meeting, Washington DC, August 15, 2012.

166. “Synthetic Biology: Putting Synthesis into Biology.” University of California, Riverside,

October 12, 2012.

167. “Challenges and Opportunities in Synthetic Biology.” DOE’s Biological and

Environmental Research Advisory Committee Meeting, Washington DC, October 15, 2012

168. “Challenges and Opportunities in Synthetic Biology.” A*STAR Scientific Conference

2012, Singapore, October 18, 2012 (Keynote lecture)

169. “Directed Evolution 20 Years on.” New England Biolabs, Ipswich, Massachusetts, October

23, 2012.

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170. “Synthetic Biology: Putting Synthesis into Biology.” Cold Spring Harbor Symposium,

Suzhou, China, November 26, 2012.

171. “Synthesis of Bio-based Chemicals Using Cell Factories.” A*STAR Workshop on Bio-

Based Chemicals, Singapore, December 3, 2012.

172. “Challenges and Opportunities in Synthetic Biology.” International Conference on Cellular

& Molecular Bioengineering III (ICCMB3), Singapore, December 8-10, 2012 (Keynote

lecture)

173. “Novel Tools for Mammalian Genome Engineering.” Department of Chemical and

Biomolecular Engineering, National University of Singapore, December 11, 2012.

174. “Synthetic Biology: Putting Synthesis into Biology.” Argonne National Laboratory,

Chicago, IL, January 17, 2013.

175. “Engineering Microbial Cell Factories for Production of Chemicals and Fuels.” The 4th

International Symposium of Innovative BioProduction Kobe (iBioK), Kobe University,

Japan, January 31, 2013.

176. “Challenges and Opportunities in Synthetic Biology.” Department of Bioengineering,

University of Illinois at Urbana-Champaign, IL, February 14, 2013.

177. “Synthetic Biology: Putting Synthesis into Biology.” Pohang University of Science and

Technology, Korea, March 22, 2013.

178. “Challenges and Opportunities in Synthetic Biology.” Korean Society of Biotechnology

and Bioengineering regional meeting, Busan, Korea, March 23, 2013.

179. “Discovery of Novel Natural Products by Refactoring Cryptic Pathways.” American

Chemical Society Annual Spring Meeting, New Orleans, April 7, 2013.

180. “Green Chemistry Enabled by Synthetic Biology.” McGill University, Montreal, Canada,

May 2, 2013.

181. “Building a Biochemical Engineering Community in Singapore.” Monthly Seminar Series

in Biochemical Engineering, Biopolis, Singapore, May 21, 2013.

182. “Discovery of Novel Natural Products by Refactoring Cryptic Pathways.” ECI Biochemical

and Molecular Engineering XVIII, Beijing, China, June 17, 2013.

183. “Synthetic Biology: Putting Synthesis into Biology.” Mini-Symposium on Synthetic

Biology, Tsinghua University, Beijing, China, June 20, 2013.

184. “Genome Engineering: A New Frontier in Synthetic Biology.” 2013 Sino-USA Chinese

Collaborative Workshop--Opportunities and Challenges in Synthetic Biology, Tianjin,

China, June 21, 2013.

185. “Discovery of Novel Natural Products by Refactoring Cryptic Pathways.” 12th International

Symposium on the Genetics of Industrial Microorganisms, Cancun, Mexico, June 24, 2013.

186. “Biocatalysis: To Be or Not to Be?” BIOTRANS 2013, Manchester, UK, July 25, 2013.

187. “Synthetic Biology: Putting Synthesis into Biology.” Stockholm University, Stockholm,

Sweden, August 7, 2013.

188. “Synthetic Biology: Putting Synthesis into Biology.” Department of Cellular and Molecular

Medicine, University of California, San Diego, August 13, 2013.

189. “Expanding the Boundaries of Biocatalysis.” Society for Industrial Microbiology and

Biotechnology Annual Meeting, San Diego, August 15, 2013.

190. “Discovery of Novel Natural Products via Synthetic Biology.” Society for Industrial

Microbiology and Biotechnology Annual Meeting, San Diego, August 15, 2013.

12

191. “Synthetic Biology: Putting Synthesis into Biology.” Department of Biomedical

Engineering, Duke University, September 5, 2013.

192. “Expanding the Boundaries of Synthetic Biology.” Department of Chemical and

Biomolecular Engineering, National University of Singapore, September 17, 2013.

193. “Protein Engineering as an Enabling Synthetic Biology Tool.” ECI Enzyme Engineering

Conference, Japan, September 24, 2013.

194. “Expanding the Boundaries of Synthetic Biology.” Department of Chemistry, University of

Chicago, September 30, 2013.

195. “Expanding the Boundaries of Synthetic Biology.” International Symposium of Korean

Society of Biotechnology and Bioengineering, October 18, 2013 (Keynote lecture)

196. “Discovery of Novel Natural Products via Synthetic Biology.” University of Illinois at

Chicago, November 8, 2013.

197. “Expanding the Boundaries of Biomolecular Engineering through Synthetic Biology.” Case

Western University, Cleveland, Ohio, November 14, 2013.

198. “Synthetic Biology: Putting Synthesis into Biology.” National University of Singapore,

Singapore, November 27, 2013.

199. “Expanding the Boundaries of Synthetic Biology: An American Perspective.” Chinese

Academy of Sciences Forum on Frontiers of Science and Technology (FFST) — Synthetic

Biology, Beijing, China, December 26, 2013.

200. “Synthetic Biology: Opportunities and Challenges.” Tsinghua University, Beijing, China,

December 27, 2013.

201. “Expanding the Boundaries of Synthetic Biology.” Shanghai JiaoTong University,

Shanghai, China, December 31, 2013.

202. “Expanding the Boundaries of Synthetic Biology.” Shanghai Chinese Academy of Sciences

Institute of Plant Physiology and Ecology, Shanghai, China, January 2, 2014.

203. “Synthetic Biology: A New Research Frontier for Chemical Engineers.” Clemson

University, South Carolina, January 16, 2014.

204. “Design and Engineering of Microbial Cell Factories for Biofuels Production.” Center for

Advanced BioEnergy Research, University of Illinois, Urbana, IL, February 24, 2014.

205. “Expanding the Boundaries of Synthetic Biology.” Elmer Gaden Award lecture, American

Chemical Society Annual Meeting, Dallas, March 18, 2014.

206. “Enabling Multistep Enzyme Processes via Synthetic Biology.” 3rd Congress on Multistep

Enzyme Catalyzed Processes (MECP14), Madrid, Spain, April 7, 2014 (Opening Keynote).

207. “Challenges and Opportunities in Synthetic Biology.” 36th Symposium on Biotechnology

for Fuels and Chemicals, Clearwater, Florida, April 30, 2014.

208. “Synthetic Biology and Biosystems Design.” Institute for Genomic Biology Fellows

Symposium, University of Illinois, Urbana, May 8, 2014.

209. “Natural Product Discovery and Development via Synthetic Biology.” Workshop on

Pharmaceutical Synthetic Biology, China Pharmaceutical University, May 19, 2014

(keynote lecture).

210. “Challenges and Opportunities in Synthetic Biology.” Chinese Academy of Sciences

Guangzhou Institute of Advanced Technologies, Guangzhou, May 26, 2014.

211. “Metabolic Engineering via RNAi-Assisted Genome Evolution (RAGE).” Society for

Biological Engineering’s Metabolic Engineering Conference X, Vancouver, Canada, June

16, 2014.

13

212. “Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB).” LanzaTech,

Chicago, IL, June 23, 2014

213. “Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB).” ADM, Decatur,

IL, June 25, 2014

214. “Discovery and Characterization of Novel Natural Products via Synthetic Biology.”

Synthetic Biology: Engineering, Evolution & Design Conference (SEED2014), Manhattan

Beach, CA, July 17, 2014.

215. “Discovery and Characterization of Novel Natural Products via Synthetic Biology.” ESF-

EMBO Conference on Synthetic Biology of Antibiotic Production II, Sant Feliu de

Guixols, Spain, September 1, 2014.

216. “Synthetic Biology: A New Frontier in Bioengineering and Biotechnology.” Frontiers in

Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, September 9,

2014.

217. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Technical

University of Hamburg, Hamburg, Germany, September 15, 2014.

218. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Innovation

Connecting Show, Toulouse, France, September 17, 2014 (Keynote lecture).

219. “Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB).” Institute of

Microbiology, Chinese Academy of Sciences, Beijing, November 1, 2014.

220. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Tsinghua

University, Beijing, China, November 2, 2014.

221. “Challenges and Opportunities in Synthetic Biology.” Indo-US Workshop on Synthetic and

Systems Biology, New Delhi, India, November 9, 2014.

222. “Challenges and Opportunities in Synthetic Biology.” AIChE Annual Meeting, Atlanta,

GA, November 17, 2014.

223. “Synthetic Biology: Challenges and Opportunities.” School of Chemical Engineering,

Zhejiang University, Hangzhou, China, December 1, 2014.

224. “Expanding the Boundaries of Synthetic Biology.” 3rd Cold Spring Harbor Asia

Conference on Synthetic Biology, Suzhou, China, December 4, 2014.

225. “Genome-scale Engineering by RNAi-Assisted Genome Evolution (RAGE).” 2014 Sino-

USA Chinese Collaborative Workshop--Opportunities and Challenges in Synthetic

Biology, Tianjin, China, December 6, 2014.

226. “Cellular Engineering by RNAi Assisted Genome Evolution (RAGE).” Korea University,

Seoul, February 6, 2015.

227. “Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB).” Biosystems

Design 1.0, Singapore, February 9, 2015.

228. “Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB).” Department of

Electrical Engineering, University of Iowa, Iowa City, March 12, 2015.

229. “Challenges and Opportunities in Synthetic Biology.” University of Manchester, UK,

March 23, 2015.

230. “Discovery of Natural Products via Synthetic Biology.” Directing Biosynthesis IV, The

John Innes Centre, Norwich, UK. March 27, 2015.

231. “Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB).” University of

Edinburg, Scotland, March 30, 2015.

14

232. “Challenges and Opportunities in Synthetic Biology.” Indiana University School of

Medicine, Indianapolis, April 14, 2015.

233. “Synthetic Biology: Putting Synthesis into Biology.” College of Pharmacy, University of

North Carolina, Chapel Hill, April 16, 2015.

234. “Exploring the Synergy between Chemical Catalysis and Biological Catalysis.” School of

Chemical Engineering, Tianjin University, May 5, 2015.

235. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” CAS Tianjin

Institute of Industrial Biotechnology, Tianjin, China, May 6, 2015 (Inaugural TIB

Distinguished Lecturer)

236. “Challenges and Opportunities in Synthetic Biology.” CAS Institute of Biophysics,

Beijing, China, May 7, 2015.

237. “Exploring the Synergy between Chemical Catalysis and Biological Catalysis.” Southeast

Asia Catalysis Conference 2015, Singapore, May 14, 2015 (plenary lecture).

238. “Challenges and Opportunities in Synthetic Biology.” Shanghai Jiao-Tong University,

Shanghai, China, June 10, 2015.

239. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Wuhan

University, Wuhan, China, June 12, 2015.

240. “Building a Biological Foundry for Next-generation Synthetic Biology.” A*STAR

Visiting Investigator Program meeting, Singapore, June 17, 2015.

241. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Temasek

Lifesciences Laboratory, Singapore, June 18, 2015.

242. “Challenges and Opportunities in Synthetic Biology.” Beijing Institute of Technology,

China, July 23, 2015.

243. “An Automated Cellular Engineering (ACE) Platform for Metabolic Engineering.”

Bioeconomy Conference, Tianjin, July 24, 2015.

244. “Discovery and Engineering of Novel Natural Products via Synthetic Biology.” Society for

Industrial Microbiology and Biotechnology annual meeting, Philadelphia, August 4, 2015

245. “Pathway Engineering via Synthetic Biology.” Society for Industrial Microbiology and

Biotechnology annual meeting, Philadelphia, August 4, 2015

246. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Center for

Biosustainability, Denmark, August 11, 2015.

247. “Enabling Multistep Enzyme Catalyzed Processes via Synthetic Biology.” ECI Enzyme

Engineering XXIII, St. Petersburg, FL, September 8, 2015.

248. “Building a Biological Foundry for Next-generation Synthetic Biology.” Shenzhen

Biotechnology Conference, Shenzhen, September 23, 2015.

249. “Building a Biological Foundry for Next-generation Synthetic Biology.” National

University of Singapore, Singapore, October 1, 2015.

250. “Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB).” Toulouse White

Biotechnology, Toulouse, Frances, October 7, 2015 (videoconference)

251. “Discovery and Engineering of Novel Natural Products via Synthetic Biology.” Eli Lilly

Research Symposium, Indianapolis, IN, November 16, 2015.

252. “Discovery and Engineering of Novel Natural Products via Synthetic Biology.” Metabolic

Engineering Summit, Beijing, China, December 2, 2015.

15

253. “Pathway Engineering via Synthetic Biology.” 2015 Sino-USA Chinese Collaborative

Workshop--Opportunities and Challenges in Synthetic Biology, Tianjin, China, December

3, 2015.

254. “Discovery and Engineering of Novel Natural Products via Synthetic Biology.”

International Conference on Metabolic Science, Shanghai, China, December 5, 2015.

255. “Enzyme Engineering as an Enabling Tool for Synthetic Biology/Chemistry.” Pacifichem,

Honolulu, HI, December 16, 2015.

256. “Discovery of Novel Natural Products via Synthetic Biology.” Pacifichem, Honolulu, HI,

December 18, 2015.

257. “Mapping the Global Landscape of Synthetic Biology: Challenges and Opportunities.”

Xiangshang Scientific Conference, Beijing, China, December 21, 2015.

258. “Challenges and Opportunities in Synthetic Biology.” Advanced Digital Sciences Center,

Singapore, January 7, 2016.

259. “Building a Biological Foundry for Next-generation Synthetic Biology.” Society for

Laboratory Automation and Screening (SLAS) annual meeting, San Diego, CA, January

25, 2016.

260. “Building a Biological Foundry for Next-generation Synthetic Biology.” Department of

Chemical and Biological Engineering, University of Colorado, Boulder, CO, January 26,

2016.

261. “Opportunities and Challenges in Synthetic Biology.” Department of Chemistry and

Biochemistry, Florida International University, Miami, FL, February 18, 2016.

262. “Building a Biological Foundry for Next-generation Synthetic Biology.” ACS Division of

Biochemical Technology annual meeting, San Diego, March 15, 2016 (keynote).

263. “Building a Biological Foundry for Next-generation Synthetic Biology.” Biosystems

Design 2.0, Singapore, March 21, 2016.

264. “Reading, Writing and Editing a Genome: An Emerging Frontier in Synthetic Biology.”

Microbiology Society Annual Conference, Liverpool, UK, March 24, 2016.

265. “Building a Biological Foundry for Next-generation Synthetic Biology.” Department of

Bioengineering, University of California, San Diego, April 5, 2016.

266. “Expanding the Boundaries of Biocatalysis.” American Society for Biochemists and

Molecular Biologists (ASBMB) annual meeting, San Diego, CA, April 6, 2016.

267. “Fully Automated Genome Engineering using a Biofoundry.” HGP-Write: A Grand

Challenge Workshop, Boston, MA, May 10, 2016.

268. “Exploring the Synergy between Chemical Catalysis and Biological Catalysis.” Centre for

Catalysis Research and Innovation (CCRI) Mini-symposium, Ottawa, Canada, May 11,

2016 (plenary lecture).

269. “Microbial Synthesis of Fuels and Chemicals.” Bioenergy and Biorefinery Conference –

Southeast Asia 2016, May 30, 2016 (plenary lecture).

270. “Discovery of Novel Natural Products via Synthetic Biology.” South China Sea Institute of

Oceanology, Chinese Academy of Sciences, Guangzhou, China, June 2, 2016.

271. “Microbial Synthesis of Fuels and Chemicals from Plant Biomass.” Corn Utilization and

Technology Conference, St. Louis, MO, June 8, 2016.

272. “Microbial Synthesis of Fuels and Chemicals via Synthetic Biology.” Lignobiotech IV,

Madrid, Spain, June 21, 2016.

16

273. “Genome-scale Engineering: A New Frontier in Metabolic Engineering.” Society for

Biological Engineering’s Metabolic Engineering XI, Kobe, Japan, June 25, 2016.

274. “Building a Biological Foundry for Next-generation Synthetic Biology.” 2016 Sino-USA

Chinese Collaborative Workshop--Opportunities and Challenges in Synthetic Biology,

Guangzhou, China, July 1, 2016.

275. “Reading, Writing and Editing a Genome: An Emerging Frontier in Synthetic Biology.”

Synthetic Biology: Engineering, Evolution & Design Conference (SEED2016), Chicago,

IL, July 19, 2016.

276. “Challenges and Opportunities in Synthetic Biology.” Tri-national Symposium on Systems

and Synthetic Biology, Urbana, IL, July 22, 2016.

277. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” SIMB Annual

Meeting, New Orleans, LA, July 28, 2016 (Charles Thom award lecture).

278. “Directed Evolution: Directing a Revolution.” Arnold Group Reunion Research

Symposium, Pasadena, CA, July 29, 2016.

279. “Development of an Automated Cellular Engineering (ACE) Platform.” Cellular Machines

Workshop, Chicago, IL, August 3, 2016.

280. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Qingdao

Institute of Bioenergy and Process Engineering, Chinese Academy of Sciences, Qingdao,

Shandong, September 20, 2016 (keynote lecture).

281. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” BASF,

Tarrytown, NY, September 29, 2016.

282. “Discovery and Engineering of Novel Natural Products via Synthetic Biology.” University

of Illinois at Chicago, IL, September 30, 2016.

283. “Genome Engineering: An Emerging Frontier in Synthetic Biology.” Department of

Chemical and Biomolecular Engineering, National Seoul University, Seoul, Korea,

October 12, 2016.

284. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Korean Society

for Biotechnology and Bioengineering (KSBB), Gwangju, Korea, October 13, 2016

(plenary lecture).

285. “Discovery of Novel Natural Products via Synthetic Biology.” 13th International

Symposium on the Genetics of Industrial Microorganisms (GIM2016), Wuhan, China,

October 17, 2016 (keynote lecture).

286. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” European

Summit of Industrial Biotechnology, Graz, Austria, November 14, 2016 (plenary lecture)

287. “Tandem Catalysis: From Biosynthesis to Biocatalysis.” AIChE annual meeting, San

Francisco, CA, November 18, 2016 (keynote lecture).

288. “Discovery and Engineering of Novel Natural Products via Synthetic Biology.” Amyris,

Emeryville, CA, November 18, 2016.

289. “Engineering Saccharomyces cerevisiae for Production of Fuels and Chemicals.”

Workshop on Second Generation Bioethanol 2016, Campinas, Brazil, November 30, 2016.

290. “Synthetic Biology: A New Engine for Industrial Biotechnology.” Tianjin University,

Tianjin, China, December 14, 2016.

291. “Genome Engineering: An Emerging Frontier in Synthetic Biology.” Cellular and

Molecular Bioengineering Annual Conference, Big Island, Hawaii, January 4, 2017.

17

292. “Building a Biological Foundry for Next-generation Synthetic Biology.” The 8th

International Symposium of Innovative BioProduction Kobe (iBioK), Kobe University,

Japan, February 2, 2017.

293. “Building a Biological Foundry for Next-generation Synthetic Biology.” Society of

Laboratory Automation and Screening annual meeting, Washington DC, February 6, 2017.

294. “Breaking the Silence: New Strategies for Discovering Novel Natural Products.”

Biosystems Design 3.0, Singapore, February 16, 2017.

295. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Colorado

School of Mines, Golden, CO, March 3, 2017.

296. “Discovery and Engineering of Novel Natural Products via Synthetic Biology.” Fusion

Conferences on Synthetic Biology for Natural Products, Cancun, Mexico, March 6, 2017.

297. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Ohio State

University, Columbus, OH, March 23, 2017.

298. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” University of

Tennessee, Knoxville, TN, April 18, 2017.

299. “Breaking the Silence: New Strategies for Discovering Novel Natural Products.” 18th

International Symposium on the Biology of Actinomycetes, Jeju, Korea, May 24, 2017.

300. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Nankai

University, China, June 26, 2017.

301. “Synthetic Biology: Tools and Applications.” Peking University, Beijing, China, June 29,

2017.

302. “Building a Biological Foundry for Next-generation Synthetic Biology.” Chinese Academy

of Sciences Shenzhen Institute of Advanced Technologies, July 6, 2017.

303. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Beijing

University of Chemical Technology, Beijing, China, July 7, 2017.

304. “Challenges and Opportunities in Synthetic Biology.” Beijing University of Chemical

Technology, China, July 7, 2017.

305. “Breaking the Silence: New Strategies for Discovering Novel Natural Products.” Gordon

Research Conference on Applied and Environmental Microbiology, South Hadley, MA,

July 16, 2017.

306. “Discovery and Engineering of Novel Natural Products via Synthetic Biology.” 2017

Microbial and Plant Systems Modulated by Secondary Metabolites Meeting, Walnut Creek,

CA, July 26, 2017.

307. “Breaking the Silence: New Strategies for Discovering Novel Natural Products.” American

Society of Pharmacognosy Annual Meeting, July 30, 2017 (Plenary).

308. “Building a Biological Foundry for Next-generation Synthetic Biology.” Society for

Industrial Microbiology and Biotechnology Annual Meeting, Denver, CO, August 2, 2017

309. “Exploring the Synergy between Biological Catalysis and Chemical Catalysis.” ACS

Annual Fall Meeting, DC, August 21, 2017.

310. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Department of

Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA, September 20,

2017.

311. “Programmable Artificial Restriction Enzymes: Discovery, Engineering and Applications.”

ECI’s Enzyme Engineering XXIV, Toulouse, France, September 25, 2017.

18

312. “Illinois Biological Foundry for Advanced Biomanufacturing.” GBSI BioPolicy Summit,

San Francisco, CA, October 16, 2017.

313. “Genome-scale Engineering: A New Frontier in Synthetic Biology.” The Enduring Legacy

of Sol Spiegelman Symposium, Urbana, IL, October 21, 2017.

314. “Breaking the Silence: New Strategies for Discovering Novel Natural Products.” Metabolic

Engineering Summit, Beijing, China, October 24, 2017.

315. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Jiangnan

University, Wuxi, China, October 25, 2017.

316. “Genome-scale Engineering: A New Frontier in Synthetic Biology.” 2017 Sino-USA

Chinese Collaborative Workshop--Opportunities and Challenges in Synthetic Biology,

Hangzhou, China, October 26, 2017.

317. “Genome-scale Engineering: A New Frontier in Metabolic Engineering.” AIChE Annual

meeting, Minneapolis, MN, October 30, 2017 (keynote).

318. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” UKSynBio

annual meeting, Manchester, United Kingdom, November 27, 2017.

319. “Genome-scale Engineering: A New Frontier in Metabolic Engineering.” 8th Asian

Symposium on Innovative Bio-Production Singapore (i-BioS), Singapore, December 19,

2017.

320. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Department of

Chemical and Biomolecular Engineering, National University of Singapore, December 21,

2017.

321. “Genome-scale Engineering: A New Frontier in Metabolic Engineering.” PepTalk 2018,

San Diego, January 10, 2018.

322. “Breaking the Silence: New Strategies for Discovering Novel Natural Products.” SIMB’s

Natural Product Discovery and Development in the Genomic Era Conference, Clearwater,

FL, January 23, 2018.

323. “Synthetic Biology: Putting Synthetic into Biology.” Department of Chemical Engineering,

Stanford University, February 5, 2018

324. “Synthetic Biology: Putting Synthetic into Biology.” Department of Biomedical

Engineering, University of California, Irvine, February 9, 2018.

325. “From Genome Engineering to Genome-scale Engineering.” Department of Biology,

Indiana State University, March 6, 2018

326. “Breaking the Silence: New Strategies for Discovering Novel Natural Products.”

Department of Chemistry, Indiana State University, March 6, 2018.

327. “Building a Fully Automated Biofoundry for Next-generation Synthetic Biology.” Joint

Genome Institute’s Synthetic Biology Strategy Meeting, San Francisco, CA, March 13,

2018.

328. “Synthetic Biology: Putting Synthetic into Biology.” Marvin Johnson Award Lecture, ACS

Annual Meeting, March 19, 2018.

329. “Synthetic Biology: A New Engine for the Third Wave of Biotechnology.” Synthetic

Biology Workshop, Beijing University of Chemical Technology, Beijing, China, April 18,

2018.

330. “Exploring the Synergy between Biological Catalysis and Chemical Catalysis.” American

Chemical Society-Chinese Chemical Society meeting, Hangzhou, China, May 7, 2018.

19

331. “Synthetic Biology: Principles, Tools, and Applications.” Zhejiang University, Beijing,

China, Hangzhou, China, May 8, 2018.

332. “Rapid Engineering of Yeast Cell Factories using Iterative Cycles of Design, Build, Test,

and Learn on a Genome Scale.” Biosystems Design symposium 4.0, Singapore, May 10,

2018.

333. “Exploring the Synergy between Biological Catalysis and Chemical Catalysis.”

Department of Chemical and Biomolecular Engineering, National University of Singapore,

Singapore, May 18, 2018.

334. “Development of Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB)

for Next Generation Synthetic Biology Applications.” Synthetic Biology: Engineering,

Evolution & Design Conference (SEED2018), Phoenix, AZ, June 5, 2018.

335. “An Update on the Development of Illinois Biological Foundry for Advanced

Biomanufacturing (iBioFAB).” Global Foundry Workshop, London, June 20, 2018.

336. “Synthetic Biology: Putting Synthesis into Biology.” University of Marburg, Marburg,

Germany, June 22, 2018.

337. “Genome-scale Engineering: A New Frontier in Metabolic Engineering.” SBE’s Metabolic

Engineering 12, Munich, Germany, June 27, 2018.

338. “Synthetic Biology: Putting Synthesis into Biology.” Shanghai Jiaotong University,

Shanghai, China, June 29, 2018.

339. “The ABC’s of Synthetic Biology.” Beijing University of Chemical Technology, July 2,

2018.

340. “Synthetic Biology: Putting Synthesis into Biology.” Beijing Institute of Technology, July

5, 2018.

341. “Genome-scale Modeling and Engineering of Yeasts for Production of Chemicals and

Biofuels.” Foundations of Systems Biology in Engineering (FOSBE), Chicago, IL, August

7, 2018.

342. “Breaking the Silence: New Strategies for Discovering Novel Natural Products.” SIMB’s

Annual Meeting, Chicago, IL, August 13, 2018.

343. “Exploring the Synergy between Biological Catalysis and Chemical Catalysis.” ACS

Annual meeting, Boston, MA, August 20, 2018.

344. “Synthetic Biology: Putting Synthesis into Biology.” Conagen, Bedford, MA, August 21,

2018.

345. “Synthetic Biology: Putting Synthesis into Biology.” Codexis, Redwood City, CA, August

23, 2018.

346. “Genome Engineering: A New Frontier in Synthetic Biology.” MilliporeSigma, St. Louis,

MO, August 24, 2018.

347. “Synthetic Biology: Putting Synthetic into Biology.” Proteus, Nimes, France, September 6,

2018.

348. “Genome-scale Engineering: A New Frontier in Synthetic Biology.” Fall Symposium 2018,

Danforth Plant Science Center, September 27, 2018.

349. “Development of Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB)

for Next Generation Synthetic Biology Applications.” Carl R. Woese Institute for Genomic

Biology, University of Illinois at Urbana-Champaign, IL, October 3, 2018.

350. “Next-generation Synthetic Biology Tools.” DOE’s Genetically Encoded Materials

Synthesis (GEMS) workshop, October 9, 2018.

20

351. “Synthetic Biology: Putting Synthesis into Biology.” Department of Chemical Engineering,

Rice University, Houston, TX, November 8, 2018.

352. “Synthetic Biology: Putting Synthesis into Biology.” Bayer, St. Louis, MO, November 30,

2018.

353. “Synthetic Biology: Putting Synthesis into Biology.” Chinese University of Hong Kong,

December 13, 2018.

354. “Discovery and Development of in vivo Biosensors.” International Symposium on

Nanobiotechnology, Biosenors, and Biochips, City University of Hong Kong, December

13, 2018.

355. “Biosystems Design by Directed Evolution.” Institute of Advanced Studies Lecture, Hong

Kong University of Science and Technology, December 14, 2018.

356. “Synthetic Biology: Putting Synthesis into Biology.” CAS Shanghai Institute of Organic

Chemistry, December 21, 2018.

357. “Synthetic Biology: Putting Synthesis into Biology.” ShanghaiTech University, December

21, 2018.

358. “Biosystems Design by Directed Evolution.” Mitsubishi Chemicals, Yokohama, Japan,

January 23, 2019.

359. “Biosystems Design by Directed Evolution.” The 10th International Symposium of

Innovative BioProduction Kobe (iBioK), Kobe University, Japan, January 24, 2019.


Illinois Institute of Technology, Chicago, IL, February 13, 2018.

361. “Synthetic Biology: Putting Synthesis into Biology.” Department of Biochemistry,

University of Missouri, Columbia, MO, March 1, 2019.

362. “Biosystems Design by Directed Evolution.” AIChE Midwest Regional Conference,

Chicago, IL, February 13, 2018 (Plenary lecture).

363. “Synthetic Biology: Putting Synthesis into Biology.” John Innes Center, Norwich, UK,

March 22, 2019.


Penn State University, State College, PA, March 29, 2019.

365. “Biosystems Design by Directed Evolution.” JGI’s Users' Annual Meeting, San Francisco,

CA, April 2, 2019.

366. “TALEN vs. CRISPR: Round 2.” Illumina Workshop on Genome Editing, Chicago, IL,

April 11, 2019.

367. “Synthetic Biology: Putting Synthesis into Biology.” Columbia University, New York

City, NY, April 23, 2019.

368. “Biosystems Design via Directed Evolution.” Biosystems Design 5.0, Singapore, May 17,

2019.

369. “Synthetic Biology: Putting Synthesis into Biology.” Zhejiang University, Hangzhou,

China, May 21, 2019.

370. “Synthetic Biology: Putting Synthesis into Biology.” National University of Singapore,

May 23, 2019.

371. “Expanding the Boundary of Biocatalysis.” Southeast Asia Catalysis Conference 2019,

Singapore, May 24, 2019 (Plenary lecture).

372. “Directed Evolution of Pathways and Genomes.” Biotrans, Netherlands, July 8, 2019.

21

373. “Rapid Engineering of Natural Product Biosynthetic Pathways.” Society for Industrial

Microbiology and Biotechnology Annual Meeting, Washington DC, July 23, 2019.

374. “Discovery of Novel Natural Products via Synthetic Biology.” Short Course on Synthetic

Biology, Cold Spring Harbor Laboratory, NY, July 30, 2019.

375. “Synthetic Biology: Putting Synthesis into Biology.” Fifth Synthetic Biology Young

Scholar Forum, Tianjin, China, August 17, 2019 (Plenary lecture)

376. “Rapid Engineering of Yeast Cell Factories using Iterative Cycles of Design, Build, Test,

and Learn on a Genome Scale.” 7th International Conference on Biorefinery,

Johannesburg, South Africa, August 19, 2019.

377. “Genome Engineering of Non-model Organisms.” 14th International Symposium on the

Genetics of Industrial Microorganisms, Pisa, Italy, September 10, 2019.

378. “Enzyme Design by Directed Evolution.” ECI’s Enzyme Engineering XXV Conference,

Whistler, Canada, September 19, 2019 (Award lecture).

379. “Biosystems Design via Directed Evolution.” University of Michigan, Ann Arbor, MI,

September 25, 2019.

380. “Discovery of Novel Natural Products via Synthetic Biology.” Beijing University, Beijing,


381. “Enzyme Engineering via Directed Evolution.” Chinese Chemical Society’s 383rd Young

Scientists’ Forum, October 21, 2019

382. “Metabolic Engineering via Directed Evolution.” Metabolic Engineering Summit 2019,

Tianjin, China, October 22, 2019.

383. “Biosystems Design via Directed Evolution.” Nankai University, Tianjin, October 22,

2019.

384. “Synthetic Biology: Putting Synthesis into Biology.” International Conference on Green

Biomanufacturing, Beijing, China, October 23, 2019.

385. “Synthetic Biology: Putting Synthesis into Biology.” College of Pharmacy, Beijing

University, China, October 24, 2019.

386. “Biosystems Design via Directed Evolution.” University of California, Santa Barbara, CA,

October 31, 2019.

387. “Biosystems Design via Directed Evolution.” East China University of Science and

Technology, Shanghai, China, November 20, 2019.

388. “Natural Product Discovery and Development via Synthetic Biology.” International

Symposium on Metabolic Sciences, Shanghai JiaoTong University, Shanghai, November

21, 2019 (Plenary lecture)

389. “Biological Synthesis of Chemicals and Fuels.” 2nd International Symposium on Biomass

Utilization Technologies (ISBUT 2019), Hangzhou, China, November 23, 2019 (Plenary

lecture).

390. “Natural Product Discovery and Development via Synthetic Biology.” Corteva,

Indianapolis, IN, December 10, 2019.

391. “Synthetic Biology: Putting Synthesis into Biology.” Huazhong Agricultural University,

Wuhan, China, December 20, 2019.

392. “Biosystems Design via Directed Evolution.” Luojia Lecture Award, Wuhan University,

Wuhan, China, December 20, 2019.

22

393. “Illinois Biological Foundry for Advanced Biomanufacturing.” NSF Workshop on Accelerating the Bioeconomy: Transformation of Engineering Biology Through Public

Biofoundries, Emeryville, CA. February 12, 2019.

394. “Synthetic Biology: Putting Synthesis into Biology.” Arizona State University, Tempe, AZ.

March 2, 2020.

395. “New High Throughput Screening Methods for Directed Enzyme Evolution.” Pittcon2020,

Chicago, March 4, 2020.

F. Offices Held in Professional Societies

Program Chair (2019), ECI’s Enzyme Engineering XXV

Conference Chair (2014-2019), Biosystems Design Annual Symposium, Singapore

Program Chair (2013), ECI’s Biochemical and Molecular Engineering XVIII

Member of Advisory Committee (2015, 2017, 2019): ECI’s Biochemical and Molecular

Engineering conferences

Member of Advisory Committee (2016, 2018, 2020): SBE’s Metabolic Engineering conferences

Member of Scientific Committee (2012), 15th International Biotechnology Symposium and

Exhibition

Organizing Committee (2012), NAE’s Indo-American Frontiers of Engineering

Program co-Chair (2011-2013), Natural Products, Society for Industrial Microbiology

Member of Organizing Committee (2011), Asian Congress of Biotechnology

Program Chair (2009), Division of Biochemical Technology, American Chemical Society

Program Chair (2009), Biocatalysis, Society for Industrial Microbiology

Program co-Chair (2007-2008), Biocatalysis, Society for Industrial Microbiology

G. Editorships of Journals or Other Learned Publications

Associate Editor, ACS Catalysis, 2010-date

Associate Editor, Journal of Industrial Microbiology and Biotechnology, 2017-date

Editorial Board Member, The CRISPR Journal, 2017-date

Editorial Board Member, Journal of Industrial Microbiology and Biotechnology, 2010-2017

Editorial Board Member, ACS Synthetic Biology, 2012-date

Editorial Board Member, Scientific Reports, 2012-date

Editorial Board Member, Biotechnology and Applied Biochemistry (BAB), 2012-date

Editorial Board Member, Biocatalysis, 2014-date

Editorial Board Member, Applied Biochemistry and Biotechnology, 2004-2008

Editorial Board Member, Engineering in Life Sciences, 2014-date

Section Editor, Current Opinion in Biotechnology, 2008

Section Editor, Manual of Industrial Microbiology and Biotechnology, ASM Press, 2008-2009

Section Editor, Current Opinion in Chemical Biology, 2014

II. PUBLICATIONS AND CREATIVE WORKS

A. Doctoral Thesis Title

Enzyme Design by Directed Evolution, 1998.

23

B. Books Authored or Co-Authored (in print or accepted)

not applicable

C. Books Edited or Co-Edited (in print or accepted)

H. Zhao (ed), Synthetic Biology: Tools and Applications. Academic Press-Elsevier, Amsterdam,

2013.

D. Chapters in Books (in print or accepted)

1. H. Zhao, J. C. Moore, A. A. Volkov and F. H. Arnold. “Methods for Optimizing Industrial

Enzymes by Directed Evolution.” In Manual of Industrial Microbiology and

Biotechnology, 2nd Ed. (A. L. Demain and J. E. Davies, Eds.) pp. 597-604, ASM Press,

Washington, DC, 1999.

2. H. Zhao and W. Zha. “Evolutionary Methods for Protein Engineering.” In Enzyme

Functionality: Design, Engineering and Screening, (A. Svendsen Ed.) pp. 353-373, Marcel

Dekker, Inc., New York, NY, 2003.

3. W. Zha, T. Zhu, and H. Zhao. “Family Shuffling with Single-stranded DNA.” In Methods

in Molecular Biology, Volume 231, pp. 93-99: Directed Evolution Library Creation:

Methods and Protocols, (F.H. Arnold and G. Georgiou, Eds.), Humana Press Inc., Totowa,

NJ, 2003.

4. O. Esteban, R. D. Woodyer, and H. Zhao. “In vitro DNA recombination by Random

Priming.” In Methods in Molecular Biology, Volume 231, pp. 101-106: Directed Evolution

Library Creation: Methods and Protocols, (F.H. Arnold and G. Georgiou, Eds.), Humana

Press Inc., Totowa, NJ, 2003.

5. H. Zhao. “A pH Indicator Based Screening Method for Hydrolytic Haloalkane

Dehalogenase.” In Methods in Molecular Biology, Volume 230, pp. 213-221: Directed

Enzyme Evolution: Screening and Selection Methods (F.H. Arnold and G. Georgiou, Eds.),

Humana Press Inc., Totowa, NJ, 2003.

6. R. D. Woodyer, T. Johannes, and H. Zhao. “Cofactor Regeneration for Biocatalytic

Applications.” In Enzyme Technology, (A. Pandey, C. Webb, C. S. Soccol, and C.

Larroche, Eds.) pp. 83-101, Asiatech Publishers, Inc., New Delhi, India, 2004.

7. T. Johannes, M. Simurdiak and H. Zhao. “Biocatalysis.” In Encyclopedia of Chemical

Processing, (S. Lee, Ed.) pp. 101-110, Marcel Dekker, Inc., New York, NY, 2006.

8. Z. Chen and H. Zhao. “Protein Design.” In Encyclopedia of Chemical Processing, (S. Lee,

Ed.) pp. 2467-2477, Marcel Dekker, Inc., New York, NY, 2006.

9. Shao, Z., Ang. E. and H. Zhao. “Biomolecular Engineering.” In Encyclopedia of Chemical

Processing, (S. Lee, Ed.) pp. 171-182, Marcel Dekker, Inc., New York, NY, 2006.

10. T. Johannes, R. Woodyer, and H. Zhao. “High Throughput Screening Methods Developed

for Oxidoreductases.” In Enzyme Assays: High-throughput Screening, Genetic Selection

and Fingerprinting, (J.L Reymond, Ed.) pp. 77-93, Wiley VCH-Verlag GmbH, Weinheim,

Germany, 2006.

11. S. Rubin-Pitel, C. M.-H. Cho, W. Chen, and H. Zhao. “Directed Evolution Tools in

Bioproduct and Bioprocess Development.” In Bioprocessing for Value-Added Products

from Renewable Resources: New Technologies and Applications, (S.-T. Yang, Ed.) pp. 49-

72, Elsevier Science, New York, NY, 2006.

24

12. F. Wen, M. McLachlan, and H. Zhao. “Novel and Improved Enzymes through Directed

Evolution.” In Wiley Encyclopedia of Chemical Biology, John Wiley & Sons, Hoboken,

NJ, 2008, DOI: 10.1002/9780470048672.wecb125.

13. M. DeSieno, J. Du, and H. Zhao. “Altering Enzyme Substrate and Cofactor Specificity via

Protein Engineering. “ In Protein Engineering Handbook, (S. Lutz, U. Bornscheuer, Eds.),

pp 777-796, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, 2009.

14. N. U. Nair and H. Zhao. “Improving Proteins by Directed Evolution.” In Handbooks for

Metabolic Engineering, (C. Smolke, Ed.) pp. 2.1-2.37, CRC Press, Taylor and Francis

Group, Boca Raton, FL, 2009.

15. M. Mclachlan, R.P. Sullivan, and H. Zhao. “Directed Enzyme Evolution and High

Throughput Screening.” In Biocatalysis for the Pharmaceutical Industry-Discovery,

Development, and Manufacturing, (J. Tao, G., Lin, and A. Liese, Eds.) pp. 45-64, John

Wiley and Sons (Asia) Pte Ltd, Singapore, 2009.

16. H. Zhao and S. K. Nair. “p-Aminobenzoate N-Oxygenase.” Handbook of Metalloproteins.

John Wiley & Sons, Inc. 2009, DOI: 10.1002/0470028637.met257.

17. F. Wen, S. Rubin-Pitel, and H. Zhao. “Engineering of Therapeutic Proteins.” In Protein

Engineering and Design, (J. Cochran and S. Park, Eds.), pp153-177, CRC Press, Taylor &

Francis Group, Boca Raton, FL, 2010.

18. M. DeSieno, C. Denard, and H. Zhao. “Combinatorial Biosynthesis for Drug Discovery

and Development.” In Chemical Biology of Enzymes in Biotech and Pharmaceutical

Applications, (W.K. Yeh, H.C. Yang, and J. R. McCarthy, Eds.), pp253-283, John Wiley &

Sons, Inc., Hoboken, NJ, 2010.

19. N. U. Nair, W. Tang, D. Eriksen, and H. Zhao. “Industrial Applications of Enzymes as

Catalysts.” In Manual of Industrial Microbiology and Biotechnology, 3rd Ed. (A. L.

Demain, R. Baltz, and J. E. Davies, Eds.), pp480-494, ASM Press, Washington, DC, 2010.

20. B. Kim, J. Du, and H. Zhao. “Strain Improvement via Evolutionary Engineering.” In

Engineering Complex Phenotypes in Industrial Strains (R. Patnaik, Ed.), pp. 111-131, John

Wiley & Sons, Inc., 2012.

21. Z. Shao, Y. Luo, and H. Zhao. “DNA Assembler Method for Construction of Zeaxanthin-

producing Sacchromyces cerevisiae Strains.” In Methods in Molecular Biology (J.L.

Barredo Fuente, Ed.), Volume 898, pp. 251-262, Humana Press Inc., Totowa, NJ, 2012.

22. N. U. Nair and H. Zhao. “Mutagenic Inverted Repeat Assisted Genome Engineering

(MIRAGE) in Saccharomyces cerevisiae: Deletion of gal7.” In Methods in Molecular

Biology, Volume 834, pp. 63-73 (Q. Chen, Ed.), Humana Press Inc., Totowa, NJ, 2012.

23. Z. Shao and H. Zhao. “Construction and Engineering of Large Biochemical Pathways via

DNA Assembler.” In Synthetic Biology, Methods in Molecular Biology, Volume 1073, pp.

85-106 (K.M. Polizzi, C. Kontoravdi, Eds.), Humana Press Inc., Totowa, NJ, 2013.

24. Y. Yuan, J. Du, and H. Zhao. “Customized Optimization of Metabolic Pathways by

Combinatorial Transcriptional Engineering (COMPACTER).” In Methods in Molecular

Biology, Volume 985, pp. 177-209 (H. Alper Ed.), Humana Press Inc., Totowa, NJ, 2013.

25. F. Wen and H. Zhao. “Construction and screening of an antigen-derived peptide library

displayed on yeast cell surface for CD4+ T cell epitope identification.” In

Immunoproteomics: Methods and Protocols, Methods in Molecular Biology, Volume 1061,

pp. 245-264 (S. Twine and K. Fulton, Eds.), Humana Press Inc., Totowa, NJ, 2013.

25

26. H. Zhao. “Introduction.” In Synthetic Biology: Tools and Applications, (H. Zhao, Ed.), pp.

xiii, Elsevier, San Diego, CA, 2013.

27. D. Eriksen, S. Li, and H. Zhao. “Pathway Engineering as an Enabling Synthetic Biology

Tool.” In Synthetic Biology: Tools and Applications, (H. Zhao, Ed.), pp. 43-62, Elsevier,

San Diego, CA, 2013.

28. R. E. Cobb, Y. Luo, T. Freestone, and H. Zhao. “Drug Discovery and Development via

Synthetic Biology.” In Synthetic Biology: Tools and Applications, (H. Zhao, Ed.), pp. 183-

206, Elsevier, San Diego, CA, 2013.

29. N. Sun and H. Zhao. “A Two-plasmid Bacterial Selection System for Characterization and

Engineering of Homing Endonucleases.” In Methods in Molecular Biology, Volume 1123,

pp. 87-96 (D. Edgell, Ed.), Humana Press Inc., Totowa, NJ, 2014.

30. Y. Liang, E.L. Ang, and H. Zhao. “Directed Evolution of Enzymes for Industrial

Biocatalysis.” In Industrial Biocatalysis, (P. Grunwald, Ed.), pp. 73-110, Pan Stanford

Publishing, Singapore, 2014.

31. Z. Shao and H. Zhao. “Manipulating Natural Product Biosynthetic Pathways via DNA

Assembler.” In Current Protocols in Chemical Biology, 6, 65-100 (2014).

32. Y. Liang, M.M. Zhang, E. Ang, and H. Zhao. “Biocatalysis for Drug Discovery and

Development.” In Biocatalysis-Green Technology, (R. Patel, Ed.) pp. 421-455, John Wiley

& Sons, Hoboken, New Jersey, 2016.

33. Y. Liang, E. Ang, and H. Zhao.. “Engineered Minicellulosomes for Consolidated

Bioprocessing.” In Handbook of Carbohydrate-Modifying Biocatalysts, (P. Grunwald, Ed.),

pp. 963-990, Pan Stanford Publishing, Singapore, 2016.

34. M. Wang and H. Zhao. “Combined and Iterative Use of Computational Design and

Directed Evolution for Protein-Ligand Binding Design.” In Methods in Molecular Biology,

Volume 1414, pp. 139-153 (B. Stoddard, Ed.), Humana Press Inc., Totowa, NJ, 2016.

35. S. Shi, H. Zhang, E. Ang, and H. Zhao. “High Throughput Screening or Selection Methods

for Evolutionary Enzyme Engineering.” In Understanding Enzymes: Function, Design,

Engineering and Analysis, (A. Svendsen, Ed.) pp. 707-744, Pan Stanford Publishing,

Singapore, 2016.

36. T. Si and H. Zhao. “RNAi-assisted Genome Evolution in Saccharomyces cerevisiae.” In

Methods in Molecular Biology, Volume 1470, pp. 183-198 (D. Azorsa and S. Arora Eds.),

Humana Press Inc., Totowa, NJ, 2016.

37. E. Garcia-Ruiz, A. Badur, C. Rao, and H. Zhao. “Characterization and Engineering of

Seaweed Degrading Enzymes for Biofuels and Biochemicals Production.” In Green

Chemistry and Sustainable Technology, (P. Lau, Ed.), pp. 99-128, Springer-Verlag GmbH

Germany, 2016.

38. E. Garcia-Ruiz, M. HamediRad, and H. Zhao. “Pathway Design, Engineering and

Optimization.” In Synthetic Biology and Metabolic Engineering, Advances in Biochemical

Engineering / Biotechnology (Springer) (H. Zhao and A. Zeng Eds), Volume 162, pp. 77-

116 (2017),

39. H. Zhao, and A. Zeng. “Preface: Exploring the Synergy Between Synthetic Biology and

Metabolic Engineering.” In Synthetic Biology and Metabolic Engineering, Advances in

Biochemical Engineering / Biotechnology (Springer) (H. Zhao and A. Zeng Eds), Volume

162, pp. V-VI (2017),

26

40. T. Si, J. Lian, and H. Zhao. “Strain Development by Whole-cell Directed Evolution.”

Directed Enzyme Evolution: Advances and Applications. (M. Alcalde, Ed.), pp. 173-200,

Springer International Publishing AG, 2017.

41. D. Eriksen, R. Chao, and H. Zhao. “Applying Advanced DNA Assembly Methods to

Generate Pathway Libraries.” In Wiley Biotech Series - Synthetic Biology, (C. Smolke,

Ed.), pp. 331-348, Wiley-VCH, 2018.

42. S. Shi, Liang Y., E. Ang, and H. Zhao. “Delta integration CRISPR-Cas (Di-CRISPR) in

Saccharomyces cerevisiae.” In Methods in Molecular Biology, Volume 1927, pp. 73-92 (C.

Santos and A. Parayil Eds.), Humana Press Inc., Totowa, NJ, 2019.

43. F. Wen, M. R. Smith, and H. Zhao. “Construction and screening of an antigen-derived

peptide library displayed on yeast cell surface for CD4+ T cell epitope identification.” In

Immunoproteomics: Methods and Protocols, Methods in Molecular Biology, Volume xxx,

pp. xxx (S. Twine and K. Fulton, Eds.), Humana Press Inc., Totowa, NJ, 2018.

44. B. Wang, H. Ren, Q. Tian, and H. Zhao. “Activation of Silent Natural Product Biosynthetic

Gene Clusters Using Synthetic Biology Tools.” Accepted.

45. T. Si, P. Xue, K. Choe, H. Zhao, J. Sweedler. “High-throughput Mass Spectrometry

Complements Protein Engineering.” In Protein Engineering: Tools and Applications.

Submitted.

46. K. Harnden, L. Vo, H. Zhao, Y. Lu. “Artificial Metalloenzyme Engineering.” In Protein

Engineering: Tools and Applications. Submitted.

E. Monographs (in print or accepted)

not applicable

F. Articles in Journals (in print or accepted)

1. Y. Shi, H. Zhao and C. Wang. “Relative Binding Free Energy Calculations of DNA to

Daunomycin and its 13-Dihydro Analogue.” International Journal of Biological

Macromolecules, 15, 247-251 (1993).

2. H. Zhao and F. H. Arnold. “Optimization of DNA Shuffling of High Fidelity

Recombination.” Nucleic Acids Research, 25, 1307-1308 (1997).

3. H. Zhao and F. H. Arnold. “Functional and Non-functional Mutations Distinguished by

Random Recombination of Homologous Genes.” Proceedings of National Academy of

Sciences of the United States of America, 94, 7997-8000 (1997). PMC21544

4. H. Zhao and F. H. Arnold. “Combinatorial Protein Design: Strategies for Screening Protein

Libraries.” Current Opinion in Structural Biology, 7, 480-485 (1997).

5. Z. Shao, H. Zhao, L. Giver and F. H. Arnold. “Random-Priming in vitro Recombination:

An Effective Tool for Directed Evolution.” Nucleic Acids Research, 26, 681-683 (1998).

6. H. Zhao, L. Giver, Z. Shao, J. A. Affholter and F. H. Arnold. “Molecular Evolution by

Staggered Extension Process (StEP) in vitro Recombination.” Nature Biotechnology, 16,

258-261 (1998).

7. H. Zhao and F. H. Arnold. “Directed Evolution Converts Subtilisin E into a Functional

Equivalent of Thermitase.” Protein Engineering, 12, 47-53 (1999).

8. H. Zhao, K. Chockalingam and Z. Chen. “Directed Evolution of Enzymes and Pathways

for Industrial Biocatalysis.” Current Opinion in Biotechnology, 13, 104-110 (2002).

27

9. Z. Chen and H. Zhao. “A Highly Efficient and Sensitive Screening Method for Trans-

activation Activity of Estrogen Receptors.” Gene, 306, 127-134 (2003).

10. J. Sun, J. A. Katzenellenbogen, H. Zhao, and B.S. Katzenellenbogen. “DNA Shuffling

Method for Generating Estrogen Receptor and Chimeras in a Yeast System.”

Biotechniques, 34, 278-288 (2003).

11. W. A. van der Donk and H. Zhao. “Recent Developments in Pyridine Nucleotide

Regeneration.” Current Opinion in Biotechnology, 14, 421-426 (2003).

12. H. Zhao and W. A. van der Donk. “Cofactor Regeneration for Use in Biocatalysis.”

Current Opinion in Biotechnology, 14, 583-589 (2003).

13. R.D. Woodyer, W. A. van der Donk and H. Zhao. “Relaxing the Nicotinamide Cofactor

Specificity of Phosphite Dehydrogenase by Rational Design.” Biochemistry, 42, 11604-

11614 (2003).

14. R. D. Woodyer, W. Chen and H. Zhao. “Outrunning Nature: Directed Evolution of

Superior Biocatalysts.” Journal of Chemical Education, 81, 126-133 (2004).

15. H. Zhao. “Staggered Extension Process (StEP) in vitro DNA Recombination.” Methods in

Enzymology, 388, 42-49 (2004).

16. W. Zha, Z. Shao, J. W. Frost, and H. Zhao. “Rational Pathway Engineering of Type I Fatty

Acid Synthase Allows Biosynthesis of Triacetic Acid Lactone from D-Glucose in vivo.”

Journal of the American Chemical Society, 126, 4534-4535 (2004).

17. O. Esteban, and H. Zhao. “Directed Evolution of Soluble Single-chain Human Class II

MHC Molecules on the Yeast Cell Surface.” Journal of Molecular Biology, 340, 81-95

(2004).

18. Z. Chen, B. S. Katzenellenbogen, J. A. Katzenellenbogen, and H. Zhao. “Directed

Evolution of Human Estrogen Receptor Variants with Significantly Enhanced Androgen

Specificity and Affinity.” Journal of Biological Chemistry, 279, 33855-33864 (2004).

19. R. Woodyer, M. Simurdiak, W. A. van der Donk, and H. Zhao. “Heterologous Expression,

Purification and Characterization of a Highly Active Xylose Reductase from Neurospora

crassa.” Applied and Environmental Microbiology, 71, 1642-1647 (2005). PMC1065158

20. Z. Chen and H. Zhao. “Rapid Creation of a Novel Protein Function by in vitro Co-

evolution.” Journal of Molecular Biology, 348, 1273-1282 (2005).

21. K. Chockalingam, Z. Chen, J. A. Katzenellenbogen, and H. Zhao. “Directed Evolution of

Specific Receptor-Ligand Pairs for Use in the Creation of Gene Switches.” Proceedings of

National Academy of Sciences of the United States of America, 102, 5691-5696 (2005).

PMC556283

22. J. Achkar, M. Xian, H. Zhao, and J. W. Frost. “Biosynthesis of Phloroglucinol.” Journal of

the American Chemical Society, 127, 5332-5333 (2005).

23. K. Chockalingam and H. Zhao. “Creating New Specific Ligand-Receptor Pairs for

Transgene Regulation.” Trends in Biotechnology, 23, 333-335 (2005).

24. E. Ang, H. Zhao, and J. P. Obbard. “Bioremediation of Persistent Organic Pollutants via

Biomolecular Engineering.” Enzyme and Microbial Technology, 37, 487-496 (2005).

25. R. Woodyer, H. Zhao, and W. A. van der Donk. “Mechanistic Investigation of a Highly

Active Phosphite Dehydrogenase Mutant and its Application for NADPH Regeneration.”

FEBS Journal, 272, 3816-3827 (2005).

28

26. T. Johannes, R. Woodyer, and H. Zhao. “Directed Evolution of a Thermostable Phosphite

Dehydrogenase for NAD(P)H Regeneration.” Applied and Environmental Microbiology,

71, 5728-5734 (2005). PMC1265921

27. J. Lee, M. Simurdiak, and H. Zhao. “Reconstitution and Characterization of

Aminopyrrolnitrin Oxidase that Catalyzes Unusual Arylamine Oxidation.” Journal of

Biological Chemistry, 280, 36719-36728 (2005).

28. Z. Chen and H. Zhao. “A Highly Sensitive Selection Method for Directed Evolution of

Homing Endonucleases.” Nucleic Acids Research, 33, e154 (2005). PMC1253837

29. J. Lee and H. Zhao. “Mechanistic Studies on the Conversion of Arylamines into Arylnitro

Compounds by Arylaminopyrrolnitrin Oxygenase: Identification of Intermediates and

Kinetic Studies.” Angewandte Chemie International Edition, 45, 622-625 (2006).

30. D. Xie, Z. Shao, J. Achkar, W. Zha, J. W. Frost, and H. Zhao. “Microbial Synthesis of

Triacetic Acid Lactone.” Biotechnology and Bioengineering, 93, 727-736 (2006).

31. R. Woodyer, W. A. van der Donk, and H. Zhao. “Optimizing a Biocatalyst for Improved

NAD(P)H Regeneration: Directed Evolution of Phosphite Dehydrogenase.” Combinatorial

Chemistry and High Throughput Screening, 9, 237-245 (2006).

32. S. Rubin-Pitel and H. Zhao. “Recent Advances in Biocatalysis by Directed Enzyme

Evolution.” Combinatorial Chemistry and High Throughput Screening, 9, 247-257 (2006).

33. T. Johannes and H. Zhao. “Directed Evolution of Enzymes and Biosynthetic Pathways.”

Current Opinion in Microbiology, 9, 261-267 (2006).

34. M. Simurdiak, J. Lee, and H. Zhao. “A New Class of Arylamine Oxygenases: Evidence

that p-Aminobenzoate N-Oxygenase (AurF) is a Diiron Enzyme and Further Mechanistic

Studies.” ChemBioChem, 7, 1169-1172 (2006).

35. J. Lee, E. Ang, and H. Zhao. “Probing the Substrate Specificity of Aminopyrrolnitrin

Oxygenase (PrnD) by Mutational Analysis.” Journal of Bacteriology, 188, 6179-6183

(2006). PMC1595372

36. W. Zha, S. Rubin-Pitel, and H. Zhao. “Characterization of the Substrate Specificity of

PhlD, a Type III Polyketide Synthase from Pseudomonas fluorescens.” Journal of


37. K. Chockalingam, J. Luba, H. S. Nick, D. N. Silverman, and H. Zhao. “Engineering and

Characterization of Human Manganese Superoxide Dismutase Mutants with High Activity

and Low Product Inhibition.” FEBS Journal, 273, 4853-4861 (2006).

38. R. Woodyer, Z. Shao, P. M. Thomas, N. L. Kelleher, J. A. V. Blodgett, W. M. Metcalf, W.

A. van der Donk, and H. Zhao. “Heterologous Production of Fosfomycin and Identification

of the Minimal Fosfomycin Biosynthetic Cluster.” Chemistry & Biology, 13, 1171-1182

(2006).

39. H. Zhao and W. Zha. “In vitro ‘Sexual’ Evolution through the PCR-based Staggered

Extension Process (StEP).” Nature Protocols, 1, 1865-1871 (2006).

40. T. Johannes, R. Woodyer, and H. Zhao. “Efficient Regeneration of NADPH using an

Engineered Phosphite Dehydrogenase.” Biotechnology and Bioengineering, 96, 18-26

(2007).

41. R. Woodyer, G. Li, H. Zhao and W. A. van der Donk. “New Insight into the Mechanism of

Methyl Transfer during the Biosynthesis of Fosfomycin.” Chemical Communications, 359-

361 (2007).

29

42. E. Ang, J.P. Obbard, and H. Zhao. “Probing the Molecular Determinants of Aniline

Dioxygenase Substrate Specificity.” FEBS Journal, 274, 928-939 (2007).

43. N. U. Nair and H. Zhao. “Biochemical Characterization of an L-Xylulose Reductase from

Neurospora crassa.” Applied and Environmental Microbiology, 73, 2001-2004 (2007).

PMC1828828

44. H. Zhao. “Directed Evolution of Novel Protein Functions.” Biotechnology and

Bioengineering, 98, 313-317 (2007).

45. J. Lee and H. Zhao. “Identification and Characterization of a Flavin:NADH Reductase

(PrnF) Involved in the Novel Two-component Arylamine Oxygenase.” Journal of

Bacteriology, 189, 8556-8563 (2007). PMC2168932

46. R. P. Sullivan and H. Zhao. “Cloning and Characterization of a Highly Active L-Arabinitol

Dehydrogenase from Neurospora crassa.” Applied Microbiology and Biotechnology, 77,

845–852 (2007).

47. M. McLachlan, T. Johannes, and H. Zhao. “Further Improvement of Phosphite

Dehydrogenase Thermostability by Saturation Mutagenesis.” Biotechnology and

Bioengineering, 99, 268-274 (2008).

48. W. Zha, S. Rubin-Pitel, and H. Zhao. “Exploiting Genetic Diversity by Directed Evolution:

Molecular Breeding of type III Polyketide Synthases Improves Productivity.” Molecular

Biosystems, 4, 246-248 (2008).

49. N. U. Nair and H. Zhao. “Evolution in Reverse: Engineering a D-Xylose-specific Xylose

Reductase.” ChemBioChem, 9, 1213-1215 (2008).

50. F. Wen, O. Esteban, and H. Zhao. “Rapid Identification of CD4+ T-cell Epitopes Using

Yeast Displaying Pathogen-derived Peptide Library.” Journal of Immunological Methods,

336, 37-44 (2008).

51. Y. Choi, H. Zhang, J. S. Brunzelle, S. Nair, and H. Zhao. “In vitro Reconstitution and

Crystal Structure of p-Aminobenzoate N-oxygenase (AurF) involved in Aureothin

Biosynthesis.” Proceedings of National Academy of Sciences of the United States of

America, 105, 6858-6863 (2008). PMC2383931

52. Z. Shao, J. A. V. Blodgett, B. T. Circello, R. Woodyer, G. Li, W. A. van der Donk, W. W.

Metcalf, and H. Zhao. “Biosynthesis of 2-Hydroxyethylphosphonate, an Unexpected

Intermediate Common to Multiple Phosphonate Biosynthetic Pathways.” Journal of


53. A. C. Eliot, B. M. Griffin, P. M. Thomas, T. W. Johannes, N. L. Kelleher, H. Zhao and W.

W. Metcalf. “Cloning, Expression, and Biochemical Characterization of Streptomyces

rubellomurinus Genes Required for Biosynthesis of Antimalarial Compound FR900098.”

Chemistry and Biology, 15, 765-770 (2008). PMC2603629

54. S. Rubin-Pitel, H. Zhang, J. S. Brunzelle, T. Vu, H. Zhao, and S. Nair. “Distinct Structural

Elements Dictate the Specificity of Type III Pentaketide Synthase from Neurospora

crassa.” Chemistry and Biology, 15, 1079-1090 (2008). PMC2610677

55. H. Zhao and W. Chen. “Chemical Biotechnology: Microbial Solutions to Global Change.”

Current Opinion in Biotechnology, 19, 541–543 (2008).

56. E. Ang, J. P. Obbard, and H. Zhao. “Directed Evolution of Aniline Dioxygenase with

Improved and Novel Activity.” Applied Microbiology and Biotechnology, 81, 1063–1070

(2009).

30

57. K. M. D. Islam, M. Dilcher, C. Thurow, I. Krimmelbein, C. Vock, V. Gonzalez, H. Zhao,

L. Tietze, and C. Gatz. “Directed Evolution of Estrogen Receptor Proteins with Altered

Ligand-binding Specificities.” Protein Engineering, Design, and Selection, 22, 45-52

(2009).

58. N. U. Nair and H. Zhao. “Mutagenic Inverted Repeat Assisted Genome Engineering

(MIRAGE).” Nucleic Acids Research, 37, e9 (2009). PMC2615605

59. Z. Shao, H. Zhao, and H. Zhao. “DNA Assembler, an in vivo Genetic Method for Rapid

Construction of Biochemical Pathways.” Nucleic Acids Research, 37, e16 (2009).

PMC2632897

60. Z. Chen, F. Wen, N. Sun, and H. Zhao. “Directed Evolution of Homing Endonuclease with

Altered Sequence Specificity.” Protein Engineering, Design, and Selection, 22, 249-256

(2009).

61. W. Zha, S. Rubin-Pitel, and H. Zhao. “Improving Cellular Malonyl-CoA Level in E. coli

via Metabolic Engineering.” Metabolic Engineering, 11, 192-198 (2009).

62. F. Wen, N. U. Nair, and H. Zhao. “Protein Engineering in Designing Tailored Enzymes and

Microorganisms for Biofuels Production.” Current Opinion in Biotechnology, 20, 412–419

(2009).

63. M. McLachlan, K. Chockalingam, K. Lai, and H. Zhao. “Directed Evolution of Orthogonal

Ligand Specificity in a Single Protein Scaffold.” Angewandte Chemie International

Edition, 48, 7783–7786 (2009).

64. W. Tang and H. Zhao. “Industrial Biotechnology: Tools and Applications.” Biotechnology

Journal, 4, 1725–1739 (2009).

65. Y. Choi, T. Johannes, M. Simurdiak, Z. Shao, H. Lu, and H. Zhao. “Cloning and

Heterologous Expression of the Spectinabilin Biosynthetic Gene Cluster from Streptomyces

spectabilis.” Molecular Biosystems, 6, 336 – 338 (2010).

66. F. Wen, J. Sun, and H. Zhao. “Yeast Surface Display of Trifunctional Minicellulosomes for

Simultaneous and Synergistic Saccharification and Fermentation of Cellulose to Ethanol.”

Applied and Environmental Microbiology, 76, 1251–1260 (2010).

67. L. Huang, Y. Xue, Y.X. He, X. Fei, Y.B. Teng, S. Rubin-Pitel, H. Zhao, and C.Z. Zhou.

“Crystal Structure and Computational Analyses Provide Insights into the Catalytic

Mechanism of 2, 4-Diacetylphloroglucinol Hydrolase PhlG from Pseudomonas

fluorescens.” Journal of Biological Chemistry, 285, 4603–4611 (2010).

68. T. W. Johannes, M. A. DeSieno, B. M. Griffin, P. Thomas, N. L. Kelleher, W. W. Metcalf,

and H. Zhao. “Deciphering the Late Biosynthetic Steps of Antimalarial Compound

FR900098.” Chemistry and Biology, 17, 57–64 (2010). PMC2819980

69. N. U. Nair, C. A. Denard, and H. Zhao. “Engineering of Enzymes for Selective Catalysis.”

Current Organic Chemistry, 14, 1870-1882 (2010).

70. B. Kim, R. Sullivan, and H. Zhao. “Cloning and Characterization of Fungal L-Arabinitol

Dehyrodenases.” Applied Microbiology and Biotechnology, 87, 1407-1414 (2010).

71. N. U. Nair and H. Zhao. “Selective Reduction of Xylose to Xylitol from a Mixture of

Hemicellulosic Sugars.” Metabolic Engineering, 12, 462–468 (2010).

72. W. Tang, Z. Li, and H. Zhao. “Inverting the Enantioselectivity of P450pyr Monooxygenase

by Directed Evolution.” Chemical Communications, 46, 5461-5463 (2010).

73. S. Rubin-Pitel, Y. Luo, J. K. Lee, and H. Zhao. “A Diverse Family of Type III Polyketide

Synthases in Eucalyptus Species.” Molecular Biosystems, 6, 1444-1446 (2010).

31

74. J. Du, S. Li, and H. Zhao. “Cloning and Characterization of Novel Xylose Specific

Transporters from Neurospora crassa and Pichia stipitis.” Molecular Biosystems, 6, 2150 –

2156 (2010).

75. B. Bae, R. P. Sullivan, H. Zhao, and S. Nair. “Structure and Engineering of L-Arabinitol

Dehydrogenase from Neurospora crassa.” Journal of Molecular Biology, 402, 230-240

(2010)

76. S. Li, J. Du, J. Sun, X. Yang, J. M. Galazka, N. L. Glass, J. H. D. Cate, and H. Zhao.

“Overcoming Glucose Repression in Mixed Sugar Fermentation by Co-expressing a

Cellulobiose Transporter and a -Glucosidase in Saccharomyces cerevisiae.” Molecular

Biosystems, 6, 2129 – 2132 (2010).

77. J. Liang, Y. Luo, and H. Zhao. “Synthetic Biology: Putting Synthesis in Biology.” Wiley

Interdisciplinary Reviews: Systems Biology and Medicine, 3, 7-20 (2011).

78. Z. Shao, Y. Luo, and H. Zhao. “Rapid Characterization and Engineering of Natural Product

Biosynthetic Pathways via DNA Assembler.” Molecular Biosystems, 7, 1056 – 1059

(2011).

79. M. K. Tiwari, J. K. Lee, H. J. Moon, and H. Zhao. “Further Biochemical Studies on

Aminopyrrolnitrin Oxygenase (PrnD).” Biochemistry and Medicinal Chemistry Letters, 21,

2873-2876 (2011).

80. B. S. Evans, Y. Chen, W. W. Metcalf, H. Zhao, and N. L. Kelleher. “Directed Evolution of

the Nonribosomal Peptide Synthetase AdmK Generates New Andrimid Derivatives in

vivo.” Chemistry and Biology, 18, 601-607 (2011).

81. J. Du, Z. Shao, and H. Zhao. “Engineering Microbial Factories for Synthesis of Value-

added Products.” Journal of Industrial Microbiology and Biotechnology, 38, 873-890

(2011).

82. F. Wen, D. K. Sethi, K. W. Wucherpfennig, and H. Zhao. “Cell Surface Display of

Functional Human MHC Class II Proteins: Yeast Display versus Insect Cell Display.”

Protein Engineering, Design, and Selection, 24, 701-709 (2011).

83. M. McLachlan, J. A. Katzenellenbogen, and H. Zhao. “A New Fluorescence

Complementation Biosensor for Detection of Estrogenic Compounds.” Bioengineering and

Biotechnology, 108, 2794–2803 (2011).

84. B. Bae, R. E. Cobb, M. DeSieno, H. Zhao, and S. Nair. “New N-Acetyltranferase Fold in

the Structure and Mechanism of the Phosphonate Biosynthetic Enzyme FrbF.” Journal of


85. M. DeSieno, W. van der Donk, and H. Zhao. “Characterization and Application of the

Fe(II) and α-Ketoglutarate Dependent Hydroxylase FrbJ.” Chemical Communications, 47,

10025 – 10027 (2011).

86. M. Wang and H. Zhao. “Microbial Synthesis of Drugs and Fuels via Synthetic Biology.”

Chinese Bulletin of Life Sciences (In Chinese), 23, 875-881 (2011).

87. J. Li, Y. Luo, J. Lee and H. Zhao. “Characterization of a Novel Type III Polyketide

Synthase from Aspergillus niger.” Biochemistry and Medicinal Chemistry Letters, 21,

6085-6089 (2011).

88. H. Zhao. “Highlights of Biocatalysis and Biomimetic Catalysis.” ACS Catalysis, 1, 1119-

1120 (2011).

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89. A. Ghosh, H. Zhao, and N. D. Price. “Genome-scale Consequences of Cofactor Balancing

in Engineered Pentose Utilization Pathways in Saccharomyces cerevisiae.” PLoS ONE,

6(11): e27316 (2011).

90. M. Tiwari, R. Singh, J. K. Lee, and H. Zhao. “Mechanistic Studies on the Flavin:NADH

Reductase (PrnF) from Pseudomonas fluorescence Involved in Arylamine Oxygenation.”

Biochemistry and Medicinal Chemistry Letters, 22, 1344-1347 (2012).

91. N. Sun, J. Liang, Z. Abil, and H. Zhao. “Optimized TAL Effector Nucleases (TALENs) for

Use in Treatment of Sickle Cell Disease.” Molecular Biosystems, 8, 1255 – 1263 (2012).

92. M. Wang, T. Si, and H. Zhao. “Biocatalyst Development by Directed Evolution.”

Bioresource Technology, 115, 117–125 (2012).

93. Z. Shao and H. Zhao. “DNA assembler: a Synthetic Biology Tool for Characterizing and

Engineering Natural Product Gene Clusters.” Methods in Enzymology, 517, 203-224

(2012).

94. J. Sun, Z. Shao, H. Zhao, F. Wen, N. Nair, J, Xu, and H. Zhao. “Cloning and

Characterization of a Panel of Constitutive Promoters for Applications in Pathway

Engineering in Saccharomyces cerevisiae.” Biotechnology and Bioengineering, 109, 2082-

2092 (2012).

95. J. Sun, F. Wen, T. Si, J. Xu, and H. Zhao. “Direct Conversion of Xylan to Ethanol by

Recombinant Saccharomyces cerevisiae Strains Displaying an Engineered

Minixylanosome.” Applied and Environmental Microbiology, 78, 3837-3845 (2012).

96. M. Tiwari, R. Singh, H. Moon, M. Jeya, H. Zhao, J. K. Lee. “Role of Conserved Gly in the

Zn-dependent Medium Chain Dehydrogenase/reductase Superfamily.” Journal of


97. R. E. Cobb and H. Zhao. “Direct Cloning of Large Genomic Sequences.” Nature

Biotechnology, 30, 405-406 (2012).

98. J. E. Hung, E. J. Fogle, H. A. Relyea, H. D. Christman, T. W. Johannes, H. Zhao, W. W.

Metcalf, and W. A. van der Donk. “Investigation of the Role of Arg301 Identified in the X-

ray Structure of Phosphite Dehydrogenase.” Biochemistry, 51, 4254−4262 (2012).

99. Y. Zou, H. Zhang, J. S. Brunzelle, T. W. Johannes, R. Woodyer, J. E. Hung, N. Nair, W. A.

van der Donk, H. Zhao, and S. K. Nair. “Crystal Structures of Phosphite Dehydrogenase

Provide Insights into Nicotinamide Cofactor Regeneration.” Biochemistry, 51, 4263−4270

(2012).

100. R. E. Cobb, T. Si, and H. Zhao. “Directed Evolution: an Evolving and Enabling Synthetic

Biology Tool.” Current Opinion in Chemical Biology, 16, 285–291 (2012).

101. J. Du, Y. Yuan, T. Si, Lian, J., and H. Zhao. “Customized Optimization of Metabolic

Pathways by Combinatorial Transcriptional Engineering (COMPACTER).” Nucleic Acids

Research, 40, e142 (2012).

102. H. Lu, E. Chanco, and H. Zhao. “CmlI is an N-Oxygenase in the Biosynthesis of

Chloramphenicol.” Tetrahedron, 68, 7651-7654 (2012).

103. N. Sun, Z. Abil, and H. Zhao. “Recent Advances in Targeted Genome Engineering in

Mammalian Systems.” Biotechnology Journal, 7, 1074-1087 (2012).

104. M. Jeya, T. Kim, M. Tiwari, J. Li, H. Zhao and J.K. Lee. “The Botrytis cinerea Type III

Polyketide Synthase Shows Unprecedented High Catalytic Efficiency toward Long Chain

Acyl-CoAs.” Molecular Biosystems, 8, 2864-2867 (2012).

http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=3CFe@d2hnb7OaHh8Mkd&page=1&doc=10

http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=3CFe@d2hnb7OaHh8Mkd&page=1&doc=10

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105. S. Wen, T. Tan, and H. Zhao. “Improving the Thermostability of Lipase Lip2 from

Yarrowia lipolytica.” Journal of Biotechnology, 164, 248– 253 (2012).

106. M. Jeya, T. Kim, M. Tiwari, J. Lin, H. Zhao and J.K. Lee. “A Type III Polyketide Synthase

from Rhizobium etli Condenses Malonyl CoAs to a Heptaketide pyrone with Unusually

High Catalytic Efficiency.” Molecular Biosystems, 8, 3103-3106 (2012).

107. J. Liang, J. Ning, and H. Zhao. “Coordinated Induction of Multi-gene Pathways in

Saccharomyces cerevisiae.” Nucleic Acids Research, 41, e54 (2013).

108. B. Kim, J. Du, D. Eriksen, and H. Zhao. “Combinatorial Design of a Highly Efficient

Xylose-Utilizing Pathway in Saccharomyces cerevisiae for the Production of Cellulosic

Biofuels.” Applied and Environmental Microbiology, 79, 931-941 (2013).

109. R. E. Cobb, R. Chao, and H. Zhao. “Directed Evolution: Past, Present and Future.” AIChE

Journal, 59, 1432-1440 (2013).

110. J. Liang, M. McLachlan, and H. Zhao. “Orthogonal Control of Endogenous Gene

Expression in Mammalian Cells Using Synthetic Ligands.” Biotechnology and

Bioengineering, 110, 1419-1429 (2013).

111. R. E. Cobb, N. Sun, and H. Zhao. “Directed Evolution as a Powerful Synthetic Biology

Tool.” Methods, 60, 81–90 (2013).

112. G. Rao, J. K. Lee, and H. Zhao. “Directed Evolution of Phloroglucinol Synthases (PhlD)

with Increased Stability for Phloroglucinol Production.” Applied Microbiology and

Biotechnology, 97, 5861-5867 (2013).

113. Y. Luo, J.K. Lee, and H. Zhao. “Challenges and Opportunities in Synthetic Biology for

Chemical Engineers.” Chemical Engineering Science, 103, 115-119 (2013).

114. N. Sun and H. Zhao. “Transcription Activator-like Effector Nucleases (TALENs): A

Highly Efficient and Versatile Tool for Genome Editing.” Biotechnology and

Bioengineering, 110, 1811-1821 (2013).

115. M. Zhang, X. Su, E.L. Ang, and H. Zhao. “Recent Advances of Biocatalyst Development

in the Pharmaceutical Industry.” Pharmaceutical Bioprocessing, 1, 179-196 (2013).

116. Y. Yuan and H. Zhao. “Directed Evolution of a Highly Efficient Cellobiose Utilizing

Pathway in an Industrial Saccharomyces cerevisiae Strain.” Biotechnology and

Bioengineering, 110, 2874-2881 (2013).

117. D. Eriksen, P.C. H. Hsieh, P. Lynn, and H. Zhao. “Directed Evolution of a Cellobiose

Utilization Pathway in Saccharomyces cervisiae Through Simultaneous Engineering of

Multiple Proteins.” Microbial Cell Factories, 12:61 (2013).

118. X. Feng and H. Zhao. “Investigating Glucose and Xylose Metabolism in Saccharomyces

cerevisiae and Scheffersomyces stipitis via 13C Metabolic Flux Analysis.” AIChE Journal,

59, 3195-3202 (2013).

119. X. Feng and H. Zhao.“Investigating Host Dependence of Xylose Utilization in

Recombinant Saccharomyces cerevisiae Strains Using RNA-Seq Analysis.” Biotechnology

for Biofuels, 6:96 (2013).

120. Z. Shao, G. Rao, C. Li, Z. Abil, Y. Luo, and H. Zhao. “Refactoring the Silent Spectinabilin

Biosynthetic Gene Cluster Using a Plug-and-Play Scaffold.” ACS Synthetic Biology, 2,

662-669 (2013).

121. S. Li, S. K. Ha, H. J. Kim, J. M. Galazka, J. H. D. Cate, and Y. S. Jin, H. Zhao.

“Investigation of the Functional Role of Aldose 1-Epimerase in Engineered Cellobiose

Utilization.” Journal of Biotechnology, 168, 1-6 (2013).

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122. C. A. Denard, J.F. Hartwig, and H. Zhao. “Multistep One-pot Reactions Combining

Biocatalysts and Chemical Catalysts for Asymmetric Synthesis.” ACS Catalysis, 3, 2856−

2864 (2013).

123. Y. Luo, H. Huang, J. Liang, M. Wang, L. Lu, Z. Shao, R.E. Cobb, and H. Zhao.

“Activation and Characterization of a Cryptic Polycyclic Tetramate Macrolactam

Biosynthetic Gene Cluster.” Nature Communications, 4: 2894 (2013).

124. X. Feng and H. Zhao.“ Investigating Xylose Metabolism in Recombinant Saccharomyces

cerevisiae via 13C Metabolic Flux Analysis.” Microbial Cell Factories, 12:114 (2013).

125. D. Eriksen, J. Lian, and H. Zhao. “Protein Design for Pathway Engineering.” Journal of

Structural Biology, 185, 234–242 (2014).

126. N. Sun and H. Zhao. “Seamless Gene Correction of the Sickle Cell Disease Mutation in

Human Induced Pluripotent Stem Cells using TAL Effector Nucleases.” Biotechnology and

Bioengineering, 111, 1048-1053 (2014).

127. N. Sun, Z. Bao, X. Xiong, and H. Zhao. “SunnyTALEN: a Second-generation TALEN

System for Human Genome Editing.” Biotechnology and Bioengineering, 111, 683-691

(2014).

128. R.E. Cobb, J. Ning, and H. Zhao. “DNA Assembly Techniques for Next Generation

Combinatorial Biosynthesis of Natural Products.” Journal of Industrial Microbiology and

Biotechnology, 41, 469-477 (2014).

129. C.A. Denard, H. Huang, M. J. Bartlett, L. Lu, Y. Tan, H. Zhao, and J.F. Hartwig.

“Cooperative Tandem Catalysis by an Organometallic Complex and a Metalloenzyme.”

Angewandte Chemie International Edition, 53, 465-469 (2014).

130. J. Liang, R. Chao, Z. Bao, N. Sun, Z. Abil, and H. Zhao.“FairyTALE: A High-throughput

TAL Effector Synthesis Platform.” ACS Synthetic Biology, 3, 67-73 (2014).

131. N. Sun and H. Zhao. “Design of a Single-chain TALEN Architecture for Genome

Engineering.” Molecular Biosystems, 10, 446 - 453 (2014).

132. M. Wang, M. Beissner, and H. Zhao. “Aryl-aldehyde Formation in Fungal Polyketides:

Discovery and Characterization of an Unprecedented Biosynthetic Mechanism.” Chemistry

and Biology, 21, 257-263 (2014).

133. T. Si, Y. Luo, and H. Xiao, and H. Zhao. “Utilizing an Endogenous Pathway for 1-Butanol

Production in Saccharomyces cerevisiae.” Metabolic Engineering, 22, 60-68 (2014).

134. J. Lian, R. Chao, and H. Zhao. “Metabolic Engineering of Saccharomyces cerevisiae Strain

Capable of Simultaneously Utilizing Glucose and Galactose to Produce Enantiopure

(2R,3R)-Butanediol.” Metabolic Engineering, 24, 139-149 (2014).

135. J. Lian, Y. Li, M. M. HamediRad, and H. Zhao. “Directed Evolution of a Cellodextrin

Transporter for Improved Biofuel Production under Anaerobic Conditions in

Saccharomyces cerevisiae.” Biotechnology and Bioengineering, 111, 1521-1531 (2014).

136. Z. Abil, C.A. Denard, and H. Zhao. “Modular Assembly of Designer PUF Proteins for

Specific Post-transcriptional Regulation of Endogenous RNA.” Journal of Biological

Engineering, 8:7 (2014).

137. S. S. Jagtap, R. Singh, H. Zhao, J. Lee. “Cloning and Characterization of a Galactitol

Dehydrogenase from Rhizobium legumenosarum and its Application in D-Tagatose

Production.” Enzyme and Microbial Technology, 58-59, 44-51 (2014).

35

138. M. Wang and H. Zhao. “Characterization and Engineering of the Adenylation Domain of a

NRPS-like Protein: a Potential Biocatalyst for Aldehyde Generation.” ACS Catalysis, 4,

1219-1225 (2014).

139. S. S. Jagtap, J.-H. Hehemann, M. F. Polz, J.K. Lee, and H. Zhao. “Comparative

Biochemical Characterization of Three Exolytic Oligoalginate Lyases from Vibrio

splendidus Revealed Complementary Substrate Scope, Temperature and pH Adaptations.”

Applied and Environmental Microbiology, 80, 4207-4214 (2014).

140. H. Xiao and H. Zhao. “Genome-wide RNAi Screen Reveals the E3 SUMO-protein Ligase

Gene SIZ1 as a Novel Determinant of Furfural Tolerance in S. cerevisiae.” Biotechnology

for Biofuels, 7:78 (2014).

141. J. Lian, T. Si, N.U. Nair, and H. Zhao. “Design and Construction of Acetyl-CoA

Overproducing Saccharomyces cerevisiae Strains.” Metabolic Engineering, 24, 139-149

(2014).

142. E. Chanco, Y. S. Choi, N. Sun, M. Vu, and H. Zhao “Characterization of the N-Oxygenase

AurF from Streptomyces thioletus.” Bioorganic & Medicinal Chemistry, 22, 5569-5577

(2014).

143. H. Hua, C. A. Denard, R. Alamillo, A. J. Crisci, Y. Miao, J. A. Dumesic, S. L. Scott and H.

Zhao. “Tandem Catalytic Conversion of Glucose to 5-Hydroxymethylfurfural with an

Immobilized Enzyme and a Solid Acid.” ACS Catalysis, 4, 2165–2168 (2014).

144. H. Xiao, Z. Shao, Y. Jiang, S. Dole, and H. Zhao. “Exploiting Issatchenkia orientalis

SD108 for Succinic Acid Production.” Microbial Cell Factories, 13:121 (2014).

145. Y. Liang, T. Si, E. Ang, and H. Zhao. “Engineered a Pentafunctional Minicellulosome for

Simultaneous Saccharification and Ethanol Fermentation in Saccharomyces cerevisiae.”

Applied and Environmental Microbiology, 80, 6677-6684 (2014).

146. C. W. Jones, D. J. Smith, T. B. Gunnoe, H. Zhao, P. Sautet, S. L. Scott, B. Xu. “Preparing

Your Manuscript for Submission to ACS Catalysis.” ACS Catalysis, 4, 2827–2828 (2014).

147. Y. Luo, R. E. Cobb, and H. Zhao. “Recent Advances in Natural Product Discovery.”

Current Opinion in Biotechnology, 30, 230–237 (2014).

148. R. Chao, Y. Yuan, and H. Zhao. “Recent Advances in DNA Assembly Technologies.”

FEMS Yeast Research, 15, 1-9 (2015).

149. T. Si, Y. Luo, Z. Bao, and H. Zhao. “RNAi-Assisted Genome Evolution in Saccharomyces

cerevisiae for Complex Phenotype Engineering.” ACS Synthetic Biology, 4, 283−291

(2015).

150. J. Lian and H. Zhao. “Reversing the β-Oxidation Cycle in Saccharomyces cerevisiae for

Synthesis of Fuels and Chemicals.” ACS Synthetic Biology, 4, 332−341 (2015).

151. Z. Abil, X. Xiong, and H. Zhao. “Synthetic Biology for Therapeutic Applications.”

Molecular Pharmaceutics, 12, 322−331 (2015).

152. Z. Bao, H. Xiao, J. Liang, X. Xiong, N. Sun, T. Si, and H. Zhao. “Homology Integrated

CRISPR-Cas (HI-CRISPR) System for One-step Multi-gene Disruptions in Saccharomyces

cerevisiae.” ACS Synthetic Biology, 4, 585−594 (2015).

153. J. Lian and H. Zhao. “Recent Advances in Biosynthesis of Fatty Acids Derived Products in

Saccharomyces cerevisiae.” Journal of Industrial Microbiology and Biotechnology,

42:437–451 (2015).

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154. H. Xiao, Z. Bao, and H. Zhao. “High Throughput Screening and Selection Methods for

Directed Enzyme Evolution.” Industrial and Engineering Chemistry Research, 54,

4011−4020 (2015).

155. X. Feng, J. Lian, and H. Zhao. “Metabolic Engineering of Saccharomyces cerevisiae to

Improve 1-Hexadecanol Production.” Metabolic Engineering, 27, 10–19 (2015).

156. T. Si, H. Xiao, and H. Zhao. “Rapid Prototyping of Microbial Cell Factories via Genome-

scale Engineering.” Biotechnology Advances, 33, 1420–1432 (2015).

157. H. Zhao and T. Tan. “Industrial Biotechnology: Tools and Applications.” Biotechnology

Advances, 33, 1393–1394 (2015).

158. R.E. Cobb, Y. Wang, and H. Zhao. “High-efficiency Multiplex Genome Editing of

Streptomyces Species using an Engineered CRISPR/Cas System.” ACS Synthetic Biology,

4, 723–728 (2015).

159. H. Sun, Z. Liu, H. Zhao, E. L. Ang. “Recent Advances in Combinatorial Biosynthesis for

Drug Discovery.” Drug Design, Development and Therapy, 9, 823-833 (2015).

160. C.A. Denard, H. Ren, and H. Zhao. “Improving and Repurposing Biocatalysts via Directed

Evolution.” Current Opinion in Chemical Biology, 25, 55-64 (2015).

161. R. E. Cobb, B. Bae, Z. Li, M.A. DeSieno, S.K. Nair, and H. Zhao. “Structure-guided

Design and Biosynthesis of a Novel FR-900098 Analogue as a Potent Plasmodium

falciparum 1-Deoxy-D-xylulose-5-phosphate Reductoisomerase (Dxr) Inhibitor.” Chemical

Communications, 51, 2526 – 2528 (2015).

162. A. H. Badur, S. S. Jagtap, G. Yalamanchali, J.-K. Lee, H. Zhao, and C.V. Rao.

“Characterization of the Alginate Lyases from Vibrio splendidus 12B01.” Applied and

Environmental Microbiology, 81, 1865-1873 (2015).

163. J. R. Dixon, I. Jung, S. Selvaraj, Y. Shen, J. E., Antosiewicz-Bourget, A. Y. Lee, Z. Ye, A.

Kim, N. Rajagopal, W. Xie, J. Liang, H. Zhao, V. V. Lobanenkov, J. Ecker, J. Thomson,

and B. Ren. “Chromatin Architecture Reorganization during Stem Cell Differentiation.”

Nature, 518, 331–336 (2015).

164. D. Eriksen, M. HamediRad, Y. Yuan, and H. Zhao. “Orthogonal Fatty Acid Biosynthetic

Pathway Improves Fatty Acid Ethyl Ester Production in Saccharomyces cerevisiae.” ACS

Synthetic Biology, 4, 808−814 (2015).

165. P. Reddy, A. Ocampo, K. Suzuki, J. Luo, S. R. Bacman, S. L. Williams, A. Sugawara, D.

Okamura, Y. Tsunekawa, D. Lam, X. Xiong, N. Monsterrat, C. Rodriguez, I. Sancho-

Martinez, D. Manau, S. Civico, M. del Mar O’callaghan, J. Campistol, H. Zhao, J. M.

Campistol, C. T. Moraes, J. C. Izpisua-Belmonte. “Selective Elimination of Mitochondrial

Mutations in the Germline by Genome Editing.” Cell, 161, 459–469 (2015).

166. L. Cuculis, Z. Abil, H. Zhao, and C. Schroeder. “Direct Observation of TALE Protein

Dynamics Reveals a Two-state Search Mechanism.” Nature Communications, 6: 7277

(2015).

167. R. Chao, Y. Yuan, and H. Zhao. “Building Biological Foundries for Next Generation

Synthetic Biology.” Science China-Life Sciences, 58, 658–665 (2015).

168. Y. Luo, L. Zhang, K. W. Barton, and H. Zhao. “Systematic Identification of a Panel of

Strong Constitutive Promoters from Streptomyces albus.” ACS Synthetic Biology, 4,

1001−1010 (2015).

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169. Y. Luo, B. Li, D. Liu, L. Zhang, Y. Chen, B. Ji, B.-X. Zeng, H. Zhao, and Y. Yuan.

“Engineered Biosynthesis of Natural Products in Heterologous Hosts.” Chemical Society

Review, 44, 5265–5290 (2015).

170. C. A. Denard, M.J. Bartlett, Y. Wang, L. Lu, J. F. Hartwig, and H. Zhao. “Development of

a One-pot Tandem Reaction Combining Ruthenium-Catalyzed Alkene Metathesis and

Enantioselective Enzymatic Oxidation to Produce Aryl Epoxides.” ACS Catalysis, 5, 3817–

3822 (2015).

171. K. Baek, J. Liang, W. T. Lim, H. Zhao, D. H. Kim, and H. Kong, “In Situ Assembly of

Antifouling/Bacterial Silver Nanoparticle- Hydrogel Composites with Controlled Particle

Release and Matrix Softening.” ACS Applied Materials & Interfaces, 7, 15359−15367

(2015).

172. D. Coursole, J. Lian, J. Shanklin, and H. Zhao. “Production of Long Chain Alcohols and

Alkanes Upon Coexpression of an Acyl-ACP Reductase and Aldehyde-Deformylating

Oxygenase with a Bacterial Type-I Fatty Acid Synthase in E. coli.” Molecular Biosystems,

11, 2464–2472 (2015).

173. Z. Abil and H. Zhao. “Engineering Reprogrammable RNA-Binding Proteins for Study and

Manipulation of the Transcriptome.” Molecular Biosystems, 11, 2658 – 2665 (2015).

174. S. Li, T. Si, H. Zhao. “Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces

cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening.” ACS Synthetic

Biology, 4, 1308−1315 (2015).

175. T. Si, M. HamediRad, and H. Zhao, “Regulatory RNA-assisted Genome Engineering in

Microorganisms.” Current Opinion in Biotechnology, 36, 85–90 (2015).

176. M. C.Y. Chang and H. Zhao. “Opportunities and Challenges in Synthetic Biology.”

Current Opinion in Chemical Biology, 25, v-vi (2015).

177. S. M. H. T. Yazdi, Y. Yuan, J. Ma, H. Zhao, and O. Milenkovic. “A Rewritable, Random-

Access DNA-Based Storage System.” Scientific Reports, 5:14138 (2015).

178. S. M. H. T. Yazdi, H. M. Kiah, E. Ruiz Garcia, J. Ma, H. Zhao, O. Milenkovic. “DNA-

Based Storage: Trends and Methods.” IEEE Transactions on Molecular, Biological and

Multi-scale Communications, 1, 230-248 (2015).

179. Z. Bao, R.E. Cobb, and H. Zhao. “Accelerated Genome Engineering through

Multiplexing.” Wiley Interdisciplinary Reviews: Systems Biology and Medicine, 8, 5–21

(2016).

180. Y. Yuan, E. Andersen, and H. Zhao. “Flexible and Versatile Strategy for Construction of

Large Biochemical Pathways.” ACS Synthetic Biology, 5, 46−52 (2016).

181. S. Shi, Y. Liang, M. Zhang, E. Ang, and H. Zhao. “A Highly Efficient Single-step,

Markerless, and Multi-copy Strategy for Chromosomal Integration of Full Biochemical

Pathways in Saccharomyces cerevisiae.” Metabolic Engineering, 33, 19-27 (2016).

182. Y. Luo, B. Enghiad, and H. Zhao. “New Tools for Reconstruction and Heterologous

Expression of Biosynthetic Gene Clusters.” Natural Product Reports, 33, 174 – 182

(2016).

183. M. Wang, S. Li, and H. Zhao. “Design and Engineering of Intracellular-Metabolite-

Sensing/Regulation Gene Circuits in Saccharomyces cerevisiae.” Biotechnology and

Bioengineering, 113, 206–215 (2016).

184. T. Freestone and H. Zhao. “Combinatorial Pathway Engineering for Optimized Production

of the Anti-malarial FR900098.” Biotechnology and Bioengineering, 113, 384–392 (2016).

38

185. M. Wang, C. Yu and H. Zhao. “Directed Evolution of Xylose Specific Transporters to

Facilitate Glucose-Xylose Co-utilization.” Biotechnology and Bioengineering, 113, 484-

491 (2016).

186. M. Wang, C. Yu, and H. Zhao. “Identification of an Important Motif that Controls the

Activity and Specificity of Sugar Transporters in Saccharomyces cerevisiae.”

Biotechnology and Bioengineering, 113, 1460-1467 (2016).

187. W. Guo, J. Sheng, H. Zhao, and X. Feng. “Metabolic Engineering of Saccharomyces

cerevisiae to Produce 1-Hexadecanol from Xylose.” Microbial Cell Factories, 15:24

(2016).

188. H. Zhang, X. Li, X. Su, E. Ang, Y. Zhang, and H. Zhao. “Production of Adipic Acid from

Sugar Beet Residue by Combined Biological and Chemical Catalysis.” ChemCatChem, 8,

1500-1506 (2016).

189. J. Lian and H. Zhao. “Functional Reconstitution of a Pyruvate Dehydrogenase in the

Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering.” ACS

Synthetic Biology, 5, 689-697 (2016).

190. Y. Wang, R. E. Cobb, and H. Zhao. “High-efficiency Genome Editing of Streptomyces

Species by an Engineered CRISPR/Cas System.” Methods in Enzymology, 575, 271-284

(2016).

191. M. M. Zhang, Y. Wang, E. Ang, and H. Zhao. “Engineering Microbial Hosts for

Production of Bacterial Natural Products.” Natural Product Reports, 33, 963–987 (2016).

192. S. Shi, T. Si, Z. Liu, H. Zhang, E. Ang, and H. Zhao. “Metabolic Engineering of an

Endogenous Pathway for n-Butanol Production in Saccharomyces cerevisiae.” Scientific

Reports, 6:25675 (2016).

193. J. Quarterman, J. M. Skerker, X. Feng, I. Y. Liu, H. Zhao, A. P. Arkin, and Y. S. Jin.

“Rapid and Efficient Galactose Fermentation by Engineered Saccharomyces cerevisiae.”

Journal of Biotechnology, 229, 13-21 (2016).

194. J. Lian, R. Jin, and H. Zhao. “Construction of Plasmids with Tunable Copy Numbers in

Saccharomyces cerevisiae and their Applications in Pathway Optimization and Multiplex

Genome Integration.” Biotechnology and Bioengineering, 113, 2462-2473 (2016).

195. T. Si, B. Li, K. Zhang, Y. Xu, H. Zhao, and J.V. Sweedler. “Characterization of Bacillus

subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging.” Journal of

Proteome, 15, 1955–1962 (2016).

196. I. Tasan, S. Jain, and H. Zhao. “Use of Genome Editing Tools to Treat Sickle Cell

Disease.” Human Genetics, 135, 1011-1028 (2016).

197. L. Cuculis, Z. Abil, H. Zhao, and C. Schroeder. “TALE Proteins Search DNA Using a

Rotationally Decoupled Mechanism.” Nature Chemical Biology, 12, 831-837 (2016).

198. Y. Liang, E. Ang, and H. Zhao. “A Step through the Looking Glass.” Science China Life

Sciences, 59, 1–2 (2016).

199. H. Zhao and M. Medema. “Standardization for Natural Product Synthetic Biology.”

Natural Product Reports, 33, 920-924 (2016).

200. K. E. Wendt, J. Ungerer, R. E. Cobb, H. Zhao and H. B. Pakrasi. “CRISPR/Cas9 Mediated

Targeted Mutagenesis of the Fast Growing Cyanobacterium Synechococcus elongatus

UTEX 2973.” Microbial Cell Factories, 15:115 (2016).

201. M. H. Medema and H. Zhao. “Editorial: Synthetic Biology and Bioinformatics.” Natural

Product Reports, 33, 913-914 (2016).

39

202. S. Chang, P. Fornasiero, T. B. Gunnoe, C.W. Jones, S. Linic, T. Ooi, R. William, and H.

Zhao. “ACS Catalysis and the Scope of Papers Sought in Three Catalysis Subdisciplines:

Biocatalysis and Enzymology, Molecular Catalysis for Organic Synthesis, and

Heterogeneous Photocatalysis.” ACS Catalysis, 6, 4782-4785 (2016).

203. T. Si and H. Zhao. “A Brief Overview of Synthetic Biology Research Programs and

Roadmap Studies in the United States.” Synthetic and Systems Biotechnology, 1, 258-264

(2016).

204. Z. Jin, T. Schwend, J. Fu, Z. Bao, J. Liang, H. Zhao, W. Mei, and J. Yang. “Members of the

Rusc Protein Family Interact with Sufu and Inhibit Vertebrate Hedgehog Signaling.”

Development, 143, 3944-3955 (2016).

205. H. Sun, W. L. Yeo, Y. H. Lim, X. Chew, D. J. Smith, B. Xue, K. P. Chan, R. C. Robinson,

E. G. Robins, H. Zhao, and E. L. Ang. “Directed Evolution of a Fluorinase for Improved

Fluorination Efficiency with a Non-native Substrate.” Angewandte Chemie International

Edition, 46, 14275-14278 (2016).

206. Y. Wang and H. Zhao. “Tandem Reactions Combining Biocatalysts and Chemical

Catalysts for Asymmetric Synthesis.” Catalysts, 6, 194 (2016).

207. T. S. Freestone, K.-S. Ju, B. Wang, and H. Zhao. “Discovery of a Phosphonoacetic Acid

Derived Natural Product by Pathway Refactoring.” ACS Synthetic Biology, 6, 217−223.

(2017).

208. Y. Liang, Z. Liu, E. Ang, and H. Zhao. “A New Era of Genome Integration – Simply Cut

and Paste!” ACS Synthetic Biology, 6, 601−609 (2017).

209. R. Chao, T. Si, J. Liang, I. Tasan, Y. Min, W. Ren, L. Ju, and H. Zhao “Fully Automated

One-Step Synthesis of Single-Transcript TALEN Pairs using a Biological Foundry.” ACS

Synthetic Biology, 6, 678−685 (2017).

210. M. M. Zhang, F. Wong, Y. Wang, S. Luo, Y. H. Lim, E. Heng, W. L. Yeo, R. E Cobb, B.

Enghiad, E. Ang, and H. Zhao. “CRISPR-Cas9 Strategy for Activation of Silent

Streptomyces Biosynthetic Gene Clusters.” Nature Chemical Biology, 13, 607-609 (2017).

211. Z. Bao, V. Jaroenpuntaruk, S. Jain, and H. Zhao. “Orthogonal Genetic Regulation in

Human Cells using Chemically Induced CRISPR/Cas9 Activators.” ACS Synthetic Biology,

6, 686−693 (2017).

212. B. Enghiad and H. Zhao. “Programmable DNA-guided Artificial Restriction Enzymes.”

ACS Synthetic Biology, 6, 752−757 (2017).

213. W. L. Yeo, X. Chew, D. J. Smith, K. P. Chan, H. Sun, H. Zhao, Y. H. Lim, and E. L. Ang.

“Probing the Molecular Determinants of Fluorinase Specificity.” Chemical

Communications, 53, 2559-2562 (2017).

214. J. Liang, Z. Liu, E. Ang, and H. Zhao. “Twin-primer Non-enzymatic DNA Assembly: an

Efficient and Accurate Multi-part DNA Assembly Method.” Nucleic Acids Research, 45:

e94 (2017).

215. T. Si, R. Chao, Y. Min, Y. Wu, W. Ren, and H. Zhao. “Automated Multiplex Genome-

scale Engineering in Yeast.” Nature Communications, 8:15187 (2017).

216. H. Ren, B. Wang, and H. Zhao. “Breaking the Silence: New Strategies for Discovering

Novel Natural Products.” Current Opinion in Biotechnology, 48, 21-27 (2017).

217. X. Xiong, Y. Zhang, J. Yan, S. Jain, S. Chee, B. Ren, and H. Zhao. “A Scalable Epitope

Tagging Approach for High Throughput ChIP-seq Analysis.” ACS Synthetic Biology, 6,

1034−1042 (2017).

40

218. Z. Abil, L.F. Gumy, H. Zhao, and C. C. Hoogenraad. “Inducible Control of mRNA

Transport using Reprogrammable RNA-binding Proteins.” ACS Synthetic Biology, 6,

950−956 (2017).

219. M. M. Zhang, Y. Qiao, E. Ang, and H. Zhao. “Using Natural Products for Drug Discovery:

the Impact of the Genomics Era.” Expert Opinion in Drug Discovery, 12, 475-487 (2017).

220. Y. Wang, M. J., Bartlett, C. A. Denard, J. F. Hartwig, and H. Zhao. “Combining Rh-

Catalyzed Diazocoupling and Enzymatic Reduction to Efficiently Synthesize

Enantioenriched 2-Substituted Succinate Derivatives.” ACS Catalysis, 7, 2548–2552

(2017).

221. H. Ren and H. Zhao. “SynV and SynX: A Story More Than DNA Synthesis.” Science

China Life Sciences, 60, 558-560 (2017).

222. H. Ren, P. Hu, and H. Zhao. “A Plug-and-Play Pathway Refactoring Workflow for Natural

Product Research in Escherichia coli and Saccharomyces cerevisiae.” Biotechnology and

Bioengineering, 114, 1847-1854 (2017).

223. R. Chao, S. Miresh, T. Si, and H. Zhao. “Engineering Biological Systems using Automated

Biofoundries.” Metabolic Engineering, 42, 98-108 (2017).

224. S. Shi, Y. W. Choi, and H. Zhao, M. H. Tan, E. L. Ang. “Discovery and engineering of a 1-

Butanol Biosensor in Saccharomyces cerevisiae.” Bioresource Technology, 245, 1343–

1351 (2017).

225. T. Si, B. Li, T. J. Comi, Y. Wu, P. Hu, Y. Wu, Y. Min, D. A. Mitchell, H. Zhao, and J. V.

Sweedler. “Profiling of Microbial Colonies for High-throughput Engineering of Multi-step

Enzymatic Reactions via Optically-guided MALDI-MS.” Journal of the American

Chemical Society, 139, 12466−12473 (2017).

226. I. Tasan and H. Zhao. “Targeting Specificity of the CRISPR/Cas9 System.” ACS Synthetic

Biology, 6, 1609–1613 (2017).

227. J. Lian, M. HamediRad, S. Hu, and H. Zhao. “Combinatorial Metabolic Engineering using

an Orthogonal Tri-functional CRISPR System.” Nature Communications, 8:1688 (2017).

228. S. Shi and H. Zhao. “Metabolic Engineering of Oleaginous Yeasts for Production of Fuels

and Chemicals.” Frontiers in Microbiology, 8:2185 (2017).

229. J. Dekker, A. S. Belmont, …, H. Zhao (member of the 4DN network), …Y. Hou. “The

4DN Project Perspective.” Nature, 549, 219-226 (2017).

230. H. Sun, H. Zhang, E. Ang, and H. Zhao. “Biocatalysis for the Synthesis of Pharmaceuticals

and Pharmaceutical Intermediates.” Bioorganic and Medicinal Chemistry, 26 1275–1284

(2018).

231. Y. Wei, E. Ang, and H. Zhao. “Recent Developments in the Application of P450 Based

Biocatalysts.” Current Opinion in Chemical Biology, 43, 1–7 (2018).

232. P. Limsakul, Q. Peng, Y. Wi, M. Allen, J. Liang, A. G. Remacle, T. Lopez, X. Ge, B. Kay,

H. Zhao, A. Y. Strongin, X. Yang, S. Lu, and Y. Wang. “Directed Evolution to Engineer

Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface.” Cell

Chemical Biology, 25, 370–379 (2018).

233. S. Shi, E. Ang, and H. Zhao. “In vivo Biosensors: Mechanisms, Development, and

Applications.” Journal of Industrial Microbiology and Biotechnology, 45, 491–516 (2018).

234. Z. Bao, M. HamediRad, P. Xue, H. Xiao, I. Tasan, R. Chao, J. Liang, and H. Zhao.

“Genome-scale Engineering of Saccharomyces cerevisiae with Single Nucleotide

Precision.” Nature Biotechnology, 36, 505–508 (2018).

41

235. Y. Wang, H. Ren, and H. Zhao. “Expanding the Boundary of Biocatalysis: Design and

Optimization of in vitro Tandem Catalytic Reactions for Biochemical Production.” Critical

Reviews in Biochemistry and Molecular Biology, 53, 115-129 (2018).

236. J. Lian, M. HamediRad, and H. Zhao. “Advancing Metabolic Engineering of

Saccharomyces cerevisiae using the CRISPR/Cas System.” Biotechnology Journal,

1700601 (2018).

237. M. HamediRad, J. Lian, H. Li, and H. Zhao. “RNAi Assisted Genome Evolution Unveils

Yeast Mutants with Improved Xylose Utilization.” Biotechnology and Bioengineering, 115,

1552-1560 (2018).

238. J. Lian, Z. Bao, S. Hu, and H. Zhao. “Engineered CRISPR/Cas9 System for Multiplex

Genome Engineering of Polyploid Industrial Yeast Strains.” Biotechnology and

Bioengineering, 115, 1630-1635 (2018).

239. S. Tapsin, M. Sun, S. Yang, H. Zhang, X. N. Lim, T. T. Susanto, J. Lin, G. S. Zeng, J. Lee,

A. Lezhava, E. L. Ang, L. H. Zhang, Y. Wang, H. Zhao, N. Nagarajan, and Y. Wan.

“Genome-wide Identification of Natural RNA Aptamers in Prokaryotes and Eukaryotes.”

Nature Communications, 9:1289 (2018).

240. C. Schultz, J. Lian, and H. Zhao. “Metabolic Engineering of Saccharomyces cerevisiae

Using a Tri-functional CRISPR/Cas System for Simultaneous Gene Activation,

Interference, and Deletion.” Methods in Enzymology, 608, 265-276 (2018).

241. J. Lian, S. Miresh, and H. Zhao. “Recent Advances in Metabolic Engineering of

Saccharomyces cerevisiae: New Tools and Their Applications.” Metabolic Engineering,

50, 85–108 (2018).

242. I. Tasan, L. Zhang, G. Sustackova, J. Kim, M. Sivaguru, M. HamediRad, Y. Wang, J.

Genova, J. Ma, A. S. Belmont, and H. Zhao. “CRISPR/Cas9-mediated Knock-in of an

Optimized TetO Repeat for Live Cell Imaging of Endogenous Loci.” Nucleic Acids

Research, 46, e100 (2018).

243. Y. H. Lim, F. T. Wong, W. L. Yeo, K. C. Ching, Y. W. Lim, E. Heng, S. Chen, D.-J. Tsai,

T.-L. Lauderdale, K.-S. Shia, Y. S. Ho, S. Hoon, E. L. Ang, M. M Zhang and H. Zhao.

“Auroramycin, a Potent Antibiotic from Streptomyces roseosporus by CRISPR-Cas9

Activation.” ChemBioChem, 19, 1714-1719 (2018).

244. Z. Litman, Y. Wang, H. Zhao, and J. F. Hartwig. “Cooperative Asymmetric Reactions

Combining Photocatalysis and Enzymatic Catalysis.” Nature, 560, 355-359 (2018).

245. L. Katz, Y. Y. Chen, R. Gonzalez, T. C. Peterson, H. Zhao, R. H. Baltz. “Synthetic Biology

Advances and Applications in the Biotechnology Industry – a Perspective.” Journal of

Industrial Microbiology and Biotechnology, 45, 449–461 (2018).

246. H. Sun, H. Zhao, and E. Ang. “A Coupled Chlorinase-fluorinase System with High

Efficiency of Trans-halogenation and a Shared Substrate Tolerance.” Chemical

Communications, 54, 9458-9461 (2018).

247. J. D. Fine, H. Y. Shpigler; A. M. Ray, N. J. Beach, A. L. Sankey, A. Cash-Ahmed, Z. Y.

Huang, I. Astrauskaite, R. Chao, H. Zhao, and G. E. Robinson. “Quantifying the Effects of

Pollen Nutrition on Honey Bee Queen Egg Laying with a New Laboratory System.” PLoS

One, 13(9): e0203444 (2018).

248. P. Wang, J. Liang, L. Z. Shi, Y. Wang, M. Ouyang, Y. Wang, P. Zhang, M. Ouyang, D.

Preece, Q. Peng, L. Shao, J. Fan, J. Sun, S. S. Li, M. W. Berns, H. Zhao, and Y. Wang.

42

“Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission Upon

Wounding.” ACS Photonics, 5, 3565−3574 (2018).

249. D. Liu, Y. Wei, X. Liu, Y. Zhou, L. Jiang, J. Yin, F. Wang, Y. Hu, A. N Nanjaraj Urs, Y.

Liu, E. L. Ang, S. Zhao, H. Zhao, and Y. Zhang. “Discovery of Indoleacetate

Decarboxylase, a New Glycyl Radical Enzyme Catalyzing Formation of the Malodorant

Skatole.” Nature Communications, 9:4224 (2018).

250. H. Ren, S. Biswas, S. Ho, W. A. van der Donk, and H. Zhao. “Rapid Discovery of

Glycocins through Pathway Refactoring in Escherichia coli.” ACS Chemical Biology, 13,

2966-2972 (2018).

251. T. Si, Q. Tian, Y. Min, L. Zhang, J. V. Sweedler, W. A. van der Donk, and H. Zhao.

“Rapid Structure-activity Screening of Lanthipeptide Analogs via In-colony Removal of

Leader Peptides in Escherichia coli.” Journal of the American Chemical Society, 140,

11884-11888 (2018).

252. R. Singh, R. Singh, D. Sivakumar, S. Kondaveeti, T. Kim, J. Li, B. H. Sung, B.K. Cho, D.

R. Kim, S. C. Kim, V. C. Kalia, Y.-H. Zhang, H. Zhao, Y.C. Kang, and J.-K. Lee. “Insights

into Cell-free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction.” ACS

Catalysis, 8, 11085–11093 (2018).

253. B. Wang, F. Guo, S. H. Dong, and H. Zhao. “Activation of Silent Biosynthetic Gene

Clusters Using Transcription Factor Decoys.” Nature Chemical Biology, 15, 111–114

(2019).

254. K. Nguyen, M. A. DeSieno, B. Bae, T. W. Johannes, R. E. Cobb, H. Zhao, and S. K. Nair.

“Characterization of the Flavin Monooxygenase Involved in Biosynthesis of the

Antimalarial FR-900098.” Organic and Biomolecular Chemistry, 17, 1506-1518 (2019).

255. Y. Zhou, Y. Wei, L. Lin, T. Xu, E. L. Ang, H. Zhao, Z. Yuchi, and Y. Zhang.

“Biochemical and Structural Investigation of Sulfoacetaldehyde Reductase from Klebsiella

oxytoca.” Biochemical Journal, 476, 733-746 (2019).

256. M. Xing, Y. Wei, Y. Zhou, J. Zhang, L. Lin, Y. Hu, M., Li, G. Hua, A. N Nanjaraj Urs, D.

Liu, C. Guo, Y. Tong, E. Ang, H. Zhao, Z. Yuchi, and Y. Zhang. “Radical-mediated C-S

Bond Cleavage in C2 Sulfonate Dissimilation by Gut and Environmental Bacteria.” Nature

Communications, 10:1609 (2019).

257. N. Hillson, M. Caddick, …, H. Zhao, P.S. Freemont. “Building a Global Alliance of

Biofoundries.” Nature Communications, 10:2040 (2019)

258. J. C. Schultz, M. Cao, and H. Zhao. “Development of a CRISPR/Cas9 System for High

Efficiency Gene Deletion in Rhodosporidium toruloides.” Biotechnology and

Bioengineering, 116, 2103–2109 (2019).

259. M. HamediRad, S. Weisberg, R. Chao, J. Lian, and H. Zhao. “Highly Efficient Single-pot

Scar-less Golden Gate Assembly.” ACS Synthetic Biology, 8, 1047-1054 (2019).

260. W. L. Yeo, E. Heng, L. L. Tan, Y. W. Lim, Y. H. Lim, S. Hoon, H. Zhao, M. M. Zhang,

and F. T. Wong. “Characterization of Cas Proteins for CRISPR-Cas Editing in

Streptomycetes.” Biotechnology and Bioengineering, 116, 2330–2338 (2019).

261. Y. Zhou, Y. Wei, A. N Nanjaraj Urs, L. Lin, T. Xu, Y. Hu, Y. Du, E. L. Ang, H. Zhao, Z.

Yuchi, and Y. Zhang. “Identification and Characterization of a New Sulfoacetaldehyde

Reductase from the Human Gut Bacterium Bifidobacterium kashiwanohense.” Bioscience

Reports, 39, BSR20190715 (2019).

43

262. M. Li, Y. Wei, J. Yin, L. Lin, Y. Zhou, G. Hua, P. Cao, E. L. Ang, H. Zhao, Z. Yuchi, and

Y. Zhang. “Biochemical and Structural Investigation of Taurine:2-oxoglutarate

Aminotransferase from Bifidobacterium kashiwanohense.” Biochemical Journal, 476,

1605-1619 (2019).

263. Y. Tong, Y. Wei, Y. Hu, E. L. Ang, H. Zhao, and Y. Zhang. “A Pathway for Isethionate

Dissimilation in Bacillus krulwichiae.” Applied and Environmental Microbiology,

85:e00793-19 (2019).

264. V. G. Tran, M. Cao, Z. Fatma, X. Song, and H. Zhao. “Development of a CRISPR/Cas9-

based Tool for Gene Deletion in Issatchenkia orientalis.” mSphere, 4:e00345-19 (2019).

265. M. Xing, Y. Wei, G. Hua, M. Li, A. N. Nanjaraj Urs, F. Wang, Y. Hu, W. Zhai, Y. Liu, E.

L. Ang, H. Zhao, and Y. Zhang. “A Gene Cluster for Taurine Sulfur Assimilation in an

Anaerobic Human Gut Bacterium.” Biochemical Journal, 476, 2271–2279 (2019).

266. H. V. Dinh, P. F. Suthers, S. H. J. Chan, Y. Shen, T. Xiao, A. Deewan, S. S. Jagtap, H.

Zhao, C. V. Rao, J. D. Rabinowitz, C. D. Maranas. “A Comprehensive Genome-scale

Model for Rhodosporidium toruloides IFO0880 Accounting for Functional Genomics and

Phenotypic Data.” Metabolic Engineering Communications, 9, e00101 (2019).

267. R. Fusan, S.B.J. Kan, and H. Zhao. “A Continuing Career in Biocatalysis: Frances H.

Arnold.” ACS Catalysis, 9, 9775−9788 (2019).

268. M. HamediRad, R. Chao, S. Weisberg, J. Lian, S. Sinha, and H. Zhao. “Towards a Fully-

Automated Algorithm-Driven Platform for Biosystems Design.” Nature Communications,

10:5150 (2019).

269. J. Lian, C. Shultz, M. Cao, M. HamediRad, and H. Zhao. “Multi-functional Genome-wide

CRISPR System for High Throughput Genotype-Phenotype Mapping.” Nature


270. J. Yin, Y. Wei, D. Liu, Y. Hu, Q. Lu, E. L. Ang, H. Zhao, and Y. Zhang. “An Extended

Bacterial Reductive Pyrimidine Degradation Pathway Enabling Nitrogen Release from β-

Alanine.” Journal of Biological Chemistry, 294, 15662–15671 (2019).

271. Y. Yu, Y. Guo, Q. Tian, H. Yeh, Y. Lan, M. Zhang, I. Tasan, S. Jain, and H. Zhao. “An

Efficient Gene Knock-in Strategy Using 5’-Modified Double-stranded DNA Donors with

Short Homology Arms.” Nature Chemical Biology, 16, 387–390 (2020).

272. Y. Wei, Y. L. Tan, E. L. Ang, and H. Zhao. “Identification and Characterization of Citrus

Peel Uronic Acid Oxidase.” Chembiochem, 21, 797 – 800 (2020).

273. W. Xu, E. Klumbys, E. L. Ang, and H. Zhao. “Emerging Molecular Biology Tools and

Strategies for Engineering Natural Product Biosynthesis.” Metabolic Engineering

Communications, 10, e00108 (2020).

274. Y. Wang, X. Yu, and H. Zhao. “Biosystems Design by Directed Evolution.” AIChE

Journal, 66, e16716 (2020).

275. L. Cuculis, C. Zhao, Z. Abil, H. Zhao, D. Shukla, C. Schroeder. “Divalent Cations Promote

TALE DNA-Binding Specificity.” Nucleic Acids Research, 48, 1406-1422 (2020).

276. H. Ren, C. Shi, and H. Zhao. “Computational Tools for Discovering and Engineering

Natural Product Biosynthetic Pathways.” iScience, 23,100795 (2020).

277. X. Huang, M. Cao, and H. Zhao. “Integrating Biocatalysis with Chemocatalysis for

Selective Transformations.” Current Opinion in Chemical Biology, 55, 161-170 (2020).

278. W. L. Yeo, E. Heng, L. L. Tan, Y. W. Lim, K. C. Ching, D.-J. Tsai, Y. W. Jhang, T. L.

Lauderdale, K.-S. Shia, H. Zhao, E. L. Ang, M. M. Zhang, Y. H. Lim, F. T. Wong.

44

“Biosynthetic Engineering of the Antifungal, Anti-MRSA Auroramycin.” Microbial Cell

Factories, 19:3 (2020).

279. D. Zhu, Y. Wei, J. Yin, Q. Lu, E. L. Ang, H. Zhao, Y. Zhang. “A Pathway for Degradation

of Uracil to Acetyl Coenzyme A in Bacillus megaterium.” Applied and Environmental

Microbiology, DOI: 10.1128/AEM.02837-19.

280. B. Wang, H. Zhao. “Unleashing the Power of Energy Storage: Engineering -oxidation

Pathways for Polyketide Production.” Synthetic and Systems Biotechnology, 5, 21-22

(2020).

281. M. Cao, Z. Fatma, X. Song, P.-H. Hsieh, V. Tran, W. Lyon, M. Sayadi, Z. Shao, Y.

Yoshikuni, and H. Zhao. “A Genetic Toolbox for Metabolic Engineering of Issatchenkia

orientalis.” Metabolic Engineering, 59, 87–97 (2020).

282. S. Parajuli, B. Kannan, R. Karan, G. Sanahuja, H. Liu, E. Garcia, D. Kumar, V. Singh, H.

Zhao, S. P. Long, J. Shanklin; F. Altpeter. “Towards Oilcane: Engineering

Hyperaccumulation of Triacylglycerol into Sugarcane Stems.” GCB Bioenergy, doi:

10.1111/gcbb.12684.

283. H. Sun, H. Zhao, and E. L. Ang. “A New Biosensor for Stilbenes and a Cannabinoid

Enabled by Genome Mining of a Transcriptional Regulator.” ACS Synthetic Biology, 9,

698-705 (2020).

284. B. Wang, F. Guo, C. Huang, and H. Zhao. “Unraveling the Iterative Type I Polyketide

Synthases Hidden in Streptomyces.” Proceedings of National Academy of Sciences of the

United States of America, 117, 8449-8454 (2020).

285. S. K. Tabatabaei, B. Wang, N. B. M. Athreya, B. Enghiad, A. G. Hernandez, C. J. Fields,

J.-P. Leburton, D. Soloveichik, H. Zhao, and O. Milenkovic. “DNA Punch Cards:

Encoding Data on Native DNA Sequences via Topological Modifications.” Nature


286. P. Xue, T. Si, S. Mishra, L. Zhang, K. Choe, J. V. Sweedler, and H. Zhao. “A Mass

Spectrometry-based High-throughput Screening Method for Engineering Fatty Acid

Synthases with Improved Production of Medium Chain Fatty Acids.” Biotechnology and

Bioengineering, doi: 10.1002/bit.27343.

287. P. Xue, T. Si, and H. Zhao. “Optically Guided Mass Spectrometry to Screen Microbial

Colonies for Directed Enzyme Evolution.” Methods in Enzymology, accepted.

288. Z. Fatma, C. Schultz, and H. Zhao. “Recent Advances in Domesticating Non-model

Microorganisms.” Biotechnology Progress, e3008 (2020).

289. S. Ye, B. Enghiad, H. Zhao, E. Takano. “Fine-tuning the Regulation of Cas9 Expression

Levels for Efficient CRISPR-Cas9 Mediated Recombination in Streptomyces.” Journal of

Industrial Microbiology & Biotechnology, 47(4), 413-423 (2020).

290. H. Ren, C. Shi, I. Bothwell, W. A. van der Donk, and H. Zhao. “Discovery and

Characterization of a Class IV Lanthipeptide with a Non-overlapping Ring Pattern.” ACS

Chemical Biology, DOI: 10.1021/acschembio.0c00267.

291. X. Huang, B. Wang, Y. Wang, G. Jiang, J. Feng, and H. Zhao. “Photoenzymatic

Enantioselective Intermolecular Radical Hydroalkylation.” Nature, DOI: 10.1038/s41586-

020-2406-6.

292. J. Liu, Y. Wei, L. Lin, L. Teng, J. Yin, Q. Lu, J. Chen, Y. Zheng, Y. Li, R. Xu, W. Zhai, Y.

Liu, Y. Liu, P. Cao, E. Ang, H. Zhao, Z. Yuchi, and Y. Zhang. “Two Radical-dependent

Mechanisms for Anaerobic Degradation of the Globally Abundant Organosulfur

45

Compound Dihydroxypropanesulfonate.” Proceedings of National Academy of Sciences of

the United States of America, accepted.

293. M. J. Volk, I. Lourentzou, S. Mishra, L. T. Vo, C. Zhai, and H. Zhao. “Biosystems Design

via Machine Learning.” ACS Synthetic Biology, DOI: 10.1021/acssynbio.0c00129.

294. F. Wang, Y. Wei, Q. Lu, E.L. Ang, H. Zhao, Y. Zhang. “A Ferredoxin-dependent

Dihydropyrimidine Dehydrogenase in Clostridium chromiireducens.” Bioscience Reports,

accepted.

G. Creative Works

not applicable

H. Bulletins, Reports, or Conference Proceedings (in print or accepted)

1. # H. Zhao, L. You, and F. H. Arnold. “Strategy for the Directed Evolution of a Peptide

Ligase.” Proceedings of the 13th Enzyme Engineering Conference, Annals of the New York

Academy of Sciences, 799, 1-6 (1996).

2. # F. H. Arnold, L. Giver, A. Gershenson, H. Zhao, and K. Miyazaki. “Directed Evolution of

Mesophilic Enzymes into Their Thermophilic Counterparts.” Annals of the New York

Academy of Sciences, 870, 400-403 (1999).

3. #N. U. Nair, Z. Shao, M. DeSieno, T. Johannes, H. Zhao, T. Lee, R.P. Sullivan, M.

McLachlan, and H. Zhao. “Towards the Cost-effective Production of Antimalarial Drug

FR900098 and Butanol.” Proceeding of Foundations of Systems Biology and Engineering

(FOSBE), Denver, CO, 2009.

I. Abstracts (in print or accepted)

1. H. Zhao, R. Woodyer, T. Johannes. “Protein engineering of phosphite dehydrogenase for the

development of a novel NAD(P)H regeneration system.” Abstracts of Papers of the

American Chemical Society 227, 130 (2004).

2. E. Ang, J. Obbard, and H. Zhao. “Engineering an Enzymatic Carbazole Denitrogenation

Pathway.” Abstracts of Papers of the American Chemical Society 229, 223 (2005).

3. E. Ang, Z. Chen, and H. Zhao. “Towards Engineering of an Androgen Receptor Equivalent

from the Human Estrogen Receptor .” Abstracts of Papers of the American Chemical

Society 229, 242 (2005).

4. R. Woodyer, T. Johannes, W. A. van der Donk, and H. Zhao. “Protein Engineering of

Phosphite Dehydrogenase for NAD(P)H Regeneration.” Abstracts of Papers of the American

Chemical Society 229, 229 (2005).

5. H. Zhao, R. Woodyer, T. Johannes, and M. Mclachlan. “Development of a novel phosphite

dehydrogenase based NAD(P)H regeneration system for industrial biocatalysis.” Abstracts of

Papers of the American Chemical Society, 230, (2006).

6. H. Zhao, K. Chockalingam, M. Mclachlan, and K. Lai. “Protein engineering of gene

switches.” Abstracts of Papers of the American Chemical Society, 230, (2006).

7. H. Zhao, Z. Chen, Y. Choi, and S. Rubin-Pitel. “Directed evolution of homing endonuclease

for gene targeting.” Abstracts of Papers of the American Chemical Society, 230, (2006).

8. H. Zhao and N. Nair. “Evolution in reverse: engineering a xylose-specific xylose reductase.”

Abstracts of Papers of the American Chemical Society, 231, (2007).

http://wos17.isiknowledge.com/?SID=W5EDPJcp2GLcAJlFBk6&Func=Abstract&doc=3/8

http://wos17.isiknowledge.com/?SID=W5EDPJcp2GLcAJlFBk6&Func=Abstract&doc=3/8

46

9. H. Zhao, Z. Shao, and R. Woodyer. “Biosynthesis of fosfomycin.” Abstracts of Papers of the

American Chemical Society, 231, (2007).

J. Book Reviews (in print or accepted)

not applicable

K. Other

(1). Patents Issued and Pending (*: from UIUC)

1. F. H. Arnold, Z. Shao, J. A. Affholter, H. Zhao, and L. Giver. Recombination of

Polynucleotide Sequences Using Random or Defined Primers. US 6,153,410 (2000).

2. F. H. Arnold, Z. Shao, H. Zhao, and L. Giver. ECB Deacylase Mutants. US 6,361,988

(2002).

*3. H. Zhao and O. Esteban, Biological Scaffold Comprising Src, Major Histocompatibility

and/or PDZ Domains for use as Diagnostic Tool in Detection of Metabolic Disorders. US

7,442,773 (2008).

*4. H. Zhao, W. A. van der Donk, W. Metcalf, T. Johannes, and R. D. Woodyer. Phosphite

Dehydrogenase Mutants for Nicotinamide Cofactor Regeneration. US 7,402,419 (2008).

*5. H. Zhao, R. D. Woodyer, M. Simurdiak, and W. A. van der Donk. Highly Active Xylose

Reductase from Neurospora crassa. US7,381,553 (2008).

*6. H. Zhao and Z. Chen. Method for Engineering a Protein by in vitro Coevolution.

US20060183159 A1 (2006).

*7. H. Zhao and K. Chockalingam. Method for Generating a Mutant Protein which Efficiently

Binds a Target Molecule. US20060199250 A1 (2006).

*8. H. Zhao and Z. Chen. Compositions and Method for Detecting Endonuclease Activity. US

2007/0042404 A1 (2007).

*9. D. Metzger, P. Chambon, H. Zhao, and J. Katzenellenbogen. Method for Controlling the

Biological Activity of a Protein in a Vertebrate Cell. US9,273,115 (2016).

*10. H. Zhao and R.P. Sullivan. Arabinitol Dehydrogenases from Neurospora crassa.

US8,114,638 (2012).

*11. H. Zhao and N. Nair. Xylose Reductase Mutants and Uses Thereof. US 8,822,661 (2014).

*12. N. L. Glass, C. Tian, W. T. Beeson IV, H. Zhao, J. Du, J. Choi, J. H. D. Cate, J. Galazka, S.

Ha, S. Kim, S. Li, and X. Yang. Methods and Compositions for Improving Sugar

Transport, Mixed Sugar Fermentation, and Production of Biofuels. US8,431,360 (2013).

*13. H. Zhao, R. Sullivan, B. Bae, and S. Nair. Mutants of L-Arabinitol 4-dehydrogenase from

Neurospora crassa. WO2011130509 A1 (2011).

*14. H. Zhao, J. Du, B. Kim, D. Eriksen, T. Si, and Y. Yuan. Combinatorial Design of Highly

Efficient Heterologous Pathways. WO2012045088 A3 (2012).

*15. J. H. D. Cate, W. T. Beeson IV, J. Galazka, H. Zhao, S. Li, Y. Jin, and S. Ha. Enhanced

Fermentation of Cellodextrins and -D-Glucose. CA2824520 A1 (2012).

*16. O. Milenkovic, S. M. H. T. Yazdi, Y. Yuan, J. Ma, H. Zhao. Re-Writable DNA-Based

Digital Storage with Random Access. US15/356,118 (2016).

*17. H. Zhao, N.U. Nair, M. Racine, R. Woodyer. Production of Xylitol From a Mixture of

Hemicellulosic Sugars. US10,106,824 B2 (2018).

*18. I. Tasan, M. Sivaguru and H. Zhao. Customizable and Realistic Optical Microscopy

Resolution Standards. TF2017-124.

47

*19. J. Lian and H. Zhao. Combinatorial Metabolic Engineering using an Orthogonal Tri-

functional CRISPR System. US16/189,683 (2018).

*20. Z. Bao and H. Zhao. A Genome-scale Method for Precision Engineering of Yeast Strains.

TF 2017-075

*21. T. Si, T. Comi, B. Li, H. Zhao, and J. V. Sweedler. Optically-guided Mass Spectrometry

Profiling of Microbial Colonies for High-throughput Screening.

*22. H. Zhao and B. Enghiad. Programmable DNA-Guided Artificial Restriction Enzymes.

US15/806,621 (2017).

*23. F.T. Wong, Y. H. Lim, M. Zhang, and H. Zhao. Highly Bioactive Glycosylated Polyene

Macrolactam Compounds.

*24. H. Zhao and B. Wang. Activation of Silent Biosynthetic Gene Clusters by a Transcription

Factor Decoy Strategy. UIUC TF2018-82.

*25. O. Milenkovic, A. Gonzalo, S. Tabatabaei, and H. Zhao. Nick-Based Storage in Native

Nucleic Acids. US16/136,066 (2018).

*26. Y. Yu, Y. Guo, and H. Zhao. End-Modified Double-Stranded DNA Donors for Gene

Knock-In. US62/716,994 (2018).

*27. C. Schultz, M. Cao, and H. Zhao. A High-Efficiency CRISPR/Cas9 Genome Editing

System in Rhodosporidium toruloides. TF2019-010

*28. V. Tran and H. Zhao. A CRISPR/Cas9-Based Tool for Genome Editing in Issatchenkia

orientalis. TF2019-014

(2). Zhao Group in the News

1. “Prospecting for Proteins.” Elizabeth K. Wilson, Chemical and Engineering News, pp. 38-

43, April 12, 2002.

2. “Molecular sex for fun and profit.” Ken Garber, Latin Trade, pp. 57-59, May 2001.

3. “Fatty acid pathway, glucose produce triacetic acid lactone” Jim Barlow, News Bureau,

UIUC, April 1, 2004.

4. “Sweeter way to make drug precursor.” In-Pharma Technologist.com, June 4, 2004.

5. “Researchers improve design of genetic on-off switches.” Jim Barlow, News Bureau,

UIUC, April 7, 2005. Also in Medical News Today (United Kingdom, April 8, 2005),

Innovations Report (Germany, April 8, 2005), Technocrat.net (April 8, 2005), and Bio.com

Weekly Newsletter (April 13, 2005), Lincolndailynews.com (June 8, 2005).

6. “By creating molecular bridge, scientists change function of a protein.” Jim Barlow, News

Bureau, UIUC, May 5, 2005. Also in Biocompare (May 6, 2005), Medical News Today

(United Kingdom, May 6, 2005), Innovations Report (Germany, May 9, 2005), Bio.com

Weekly Newsletter (May 11, 2005), and Science Daily (May 17, 2005).

7. “Scientists use ‘bridge’ to change protein function.” BioScience World (Canada, May 10,

2005).

8. “Researchers develop new way to make proteins.” Greg Kline, The News-Gazette, June 6,

2005.

9. “Cloning techniques produce FDA-approved Antibiotic.” Kristen Aramthanapon, News

Bureau, UIUC, November 27, 2006. Also in Unite Press International (November 28,

2006), Biology News Net (Canada, November 28, 2006), Innovation Report (Germany,

November 28, 2006), PhysOrg.com (November 28, 2006), and Daily India (November 28,

2006).

http://www.in-pharmatechnologist.com/news/news-ng.asp?id=51196-sweeter-way-to

http://www.igb.uiuc.edu/news/in_the_news.html

http://www.igb.uiuc.edu/news/in_the_news.html

http://www.bioscienceworld.ca/view.html?id=1623

http://www.news-gazette.com/localnews/story.cfm?Number=18366

http://www.news.uiuc.edu/news/06/1127fosfomycin.html

48

10. “UI researchers using faster-growing bacteria for antibiotics.” Greg Kline, The News-

Gazette, December 10, 2006.

11. “Scientists use cloning to create antibiotic.” LAS News, College of Liberal Arts and

Sciences, UIUC, January 3, 2007.

12. “New way to make malaria medicine also first step in finding new antibiotics.” Melissa

Edwards, News Bureau, UIUC, September 26, 2008. Also in EurekAlert (September 26,

2008), Science Daily (September 26, 2008), ZAMP Bionews (September 29, 2008),

UPI.com (October 1, 2008), Pharmaceutical Formulation and Quality (October/November

2008)

13. “Huimin Zhao: Taking Nature’s evolution, and improving upon it.” EBInsider, November

08, 2008.

14. “Research Team Uncovers New Antibiotics in Unexplored Class of Compounds.” LAS

News, College of Liberal Arts and Sciences, UIUC, May 14, 2009.

15. “Sweet Yeast.” The Scientist, page 59, July 2010.

16. “Finding Sweet Spot of Yeast is Synthetic Biologist Zhao’s Quest.” EBI Bulletin, Volume

5, Issue 1, Spring 2011.

17. “New sensors streamline detection of estrogenic compounds.” UI News, UIUC, August 25,

2011.

18. “Illinois Engineering Professor Awarded Guggenheim Fellowship.” UI News, UIUC, April

12, 2012

19. “The Tale of the TALEs.” Science, News Focus, December 14, 2013.

20. “New method of DNA editing allows synthetic biologists to unlock secrets of a bacterial

genome.” Institute for Genomic Biology, UIUC, December 15, 2013.

21. “Data Storage on DNA Can Keep It Safe for Centuries.” New York Times, December 3,

2015.

22. “New Method of Genetic Engineering Indispensable Tool in Biotechnological

Applications.” Institute for Genomic Biology, UIUC, February 10, 2017.

23. “Revolutionizing Biotechnology with Artificial Restriction Enzymes.” Genetic Engineering

and Biotechnology News, February 10, 2017.

24. “CRISPR Mines Bacterial Genome for Hidden Pharmaceutical Treasure.” Illinois News

Bureau, UIUC, April 10, 2017.

25. “CRISPR Awakens Sleeping Genes, Brings Drug Candidates to Light.” Genetic

Engineering and Biotechnology News, April 11, 2017.

26. “Researchers Develop New Capabilities for Genome-wide Engineering of Yeast.” Carl R.

Woese Institute for Genomic Biology, UIUC, May 4, 2017.

27. “Expanding the Potential of Metabolic Engineering.” Carl R. Woese Institute for Genomic

Biology, UIUC, November 20, 2017.

28. “Retooling Yeast Factories.” Saccharomyces Genome Database New and Noteworthy,

December 13, 2017.

29. “New CRISPR Technology ‘Knock-out’ Yeast Genes with Single Point Precision.” Illinois

News Bureau, UIUC, May 7, 2018.

30. “A Unique Combination of Catalysts Opens Doors to Making Useful Compounds.” Carl R.

Woese Institute for Genomic Biology, UIUC, August 16, 2018.

31. “Catalytic Teamwork Transforms Alkenes Selectively.” Chemical and Engineering News,

August 19, 2018.

http://www.news-gazette.com/news/local/2006/12/10/ui_researchers_using_faster-growing_bacteria_for_antibiotics

http://pull.xmr3.com/p/1203-0496/98566930/clickto4_u-news-2007spring-07jan_clone.html

http://app.streamsend.com/c/4108982/7282/GObDz0A/L71M?redirect_to=http%3A%2F%2Fwww.las.illinois.edu%2Fnews%2F2009%2Fantibiotics%2F%3Futm_source%3Dstreamsend%26utm_medium%3Demail%26utm_content%3D4108982%26utm_campaign%3DLAS%2520News%2520Online

http://news.illinois.edu/news/11/0825estrogen_sensor_HuiminZhao.html

http://news.illinois.edu/news/12/0412guggenheim_HuiminZhao.html

http://www.igb.illinois.edu/news/dna-editing-method-allows-biologists-unlock-secrets-bacterial-genome

http://www.igb.illinois.edu/news/dna-editing-method-allows-biologists-unlock-secrets-bacterial-genome

http://www.nytimes.com/2015/12/04/science/data-storage-on-dna-can-keep-it-safe-for-centuries.html?smid=tw-share&_r=0

https://www.igb.illinois.edu/misc_news/new-genetic-engineering-method-indispensable-biotechnological-tool

https://www.igb.illinois.edu/misc_news/new-genetic-engineering-method-indispensable-biotechnological-tool

http://www.genengnews.com/gen-news-highlights/revolutionizing-biotechnology-with-artificial-restriction-enzymes/81253861?utm_medium=newsletter&utm_source=GEN+Daily+News+Highlights&utm_content=01&utm_campaign=GEN+Daily+News+Highlights_20170210

https://news.illinois.edu/blog/view/6367/486265

http://www.genengnews.com/gen-news-highlights/crispr-awakens-sleeping-genes-brings-drug-candidates-to-light/81254182

https://www.igb.illinois.edu/misc_news/new-capabilities-genome-wide-engineering-yeast

http://www.igb.illinois.edu/article/expanding-potential-metabolic-engineering

https://www.yeastgenome.org/blog/retooling-yeast-factories

https://news.illinois.edu/view/6367/647765

https://www.igb.illinois.edu/article/unique-combination-catalysts-opens-doors-making-useful-compounds

https://cen.acs.org/synthesis/catalysis/Catalytic-teamwork-transforms-alkenes-selectively/96/i33

49

32. “Unmuting large silent genes lets bacteria produce new molecules, potential drug

candidates.” UI News, UIUC, December 31, 2018.

33. “Project aims to revive natural product discovery.” Carl R. Woese Institute for Genomic

Biology, UIUC, April 23, 2019.

34. “Artificial intelligence to run the chemical factories of the future.” Illinois News Bureau,

UIUC, November 13, 2019.

35. “Robotic system can plan and perform biosynthesis without human intervention.”

Chemistry World, November 27, 2019.

36. “Researchers Shed Light on New Enzymatic Reaction.” DOE Center for Advanced

Biofuels and Bioproducts, June 8, 2020.

(3) Awards and Honors by Students 1. Zhilei Chen

Best Oral Presentation, The 17th CMG/MBTG Research Symposium, Urbana, IL, 2004

Eugene Rabinowitch Graduate Fellowship, Center for Biophysics and Computational

Biology, 2006

2. Karuppiah Chockalingam

Best Oral Presentation, The 18th CMG/MB Research Symposium, Urbana, IL, 2005

Second Best Poster Award, GSAC Research Symposium, Urbana, IL, 2005

Best Poster Award, UIUC Biotechnology Job Fair, Urbana, IL, 2005

3. Jung-Kul Lee

Shen Postdoc Fellowship, 2004-2005

4. Ryan Woodyer

W. H. Peterson Award for Best Student Poster Presentation, ACS Biochemical

Technology Division, 2005

Henry Drickamer Graduate Fellowship, Department of Chemistry, UIUC, 2004-2005

5. Sheryl Rubin-Pitel

NSF Graduate Research Fellowship, 2005-2007

Second Place Award for Poster Presentation, 7th GSAC Research Symposium, Urbana,

IL, 2008

Hanratty Travel Award, Department of Chemical and Biomolecular Engineering,

UIUC, 2008

6. Tyler Johannes

Best Poster Award, 4th GSAC Research Symposium, Urbana, IL, 2005

Mavis Memorial Scholarship Fellowship, College of Engineering, UIUC, 2006-2007

First Place Award for Poster Presentation, 6th GSAC Research Symposium, Urbana, IL,

2007

7. Fei Wen

Second Place Award for Poster Presentation, GSAC Research Symposium, Urbana, IL,

2006


UIUC, 2009

8. Matthew DeSieno

NIH Chemistry and Biology Interface Trainee, 2007-2009



https://www.igb.illinois.edu/article/project-aims-revive-natural-product-discovery


https://www.chemistryworld.com/news/robotic-system-can-plan-and-perform-biosynthesis-without-human-intervention/4010758.article

https://cabbi.bio/researchers-shed-light-on-new-enzymatic-reaction/

50


UIUC, 2009

9. Victor Gonzalez


10. Nikhil Nair

Mavis Memorial Scholarship, College of Engineering, UIUC, 2008-2009

Henry Drickamer Graduate Fellowship, Department of Chemical and Biomolecular

Engineering, UIUC, 2008-2009

Third Place Award for Oral Presentation, 7th GSAC Research Symposium, Urbana, IL,

2008

Best Oral Presentation, The 21st CMG/MBTG Research Symposium, Urbana, IL, 2008


UIUC, 2008


UIUC, 2009

Third Place Award for Oral Presentation, 8th GSAC Research Symposium, Urbana, IL,

2009

Graduate College Travel Award, UIUC, Urbana, IL 2009

11. Ryan Sullivan

First Place Award for Oral Presentation, 7th GSAC Research Symposium, Urbana, IL,

2008

12. Ryan Cobb



UIUC, 2011


2011



13. Dawn Eriksen

NSF Graduate Research Fellowship, 2008-2011


UIUC, 2009

Outstanding Poster Presentation, The 22nd CMG/MBTG Research Symposium, Urbana,

IL, 2009

Excellence in Bioengineering Award, Bioengineering @ Illinois Day Graduate Student

Poster Competition, Urbana, IL, 2010

Best Poster Award, Society for Industrial Microbiology and Biotechnology Annual

Meeting, 2013

14. Vincent Ip

Donald B. Keyes Award, Chemical and Biomolecular Engineering, UIUC, 2008

15. Luigi Chanco

NIH Molecular Biophysics Training Grant, 2009-2011

16. Jing Du

51

Chia-Chen Chu Graduate Fellowship, School of Chemical Sciences, UIUC 2009-2010




UIUC, 2010

17. Yunzi Luo


UIUC, 2010


2012

Widiger Graduate Fellowship, 2013

Chia-Chen Chu Graduate Fellowship, School of Chemical Sciences, UIUC 2013-2014


UIUC, 2014

18. Sijin Li


UIUC, 2011


UIUC, 2010


2010

19. Jing Liang

Singapore ASTAR Fellowship, 2008-2013

20. Ning Sun

Merck Poster Award (first place), ECI Enzyme Engineering Conference XXI, 2011

Richard Gumport Travel Award, 2011

Baxter International Young Investigator Award, 2013

Department of Biochemistry Trust of Urbana Award for Best Ph.D. Thesis, 2014

21. Carl Denard

Dow Graduate Fellow, 2012

22. Jiazhang Lian

3M Graduate Fellow, 2012-2013

Best Poster Award, IGB Fellows Symposium, UIUC, 2014




UIUC, 2014

Travel Award to attend the 2015 Global Young Scientists Summit in Singapore

Energy Biosciences Institute Conference Travel Award, 2014


UIUC, 2015


23. Xueyang Feng


52

24. Tong Si

Third Place Poster Award, ECI Biochemical and Molecular Engineering Conference

XVIII, 2013

Mavis Future Faculty Fellow, 2013

Graduate College Travel Award, 2013




Best Poster Award, IGB Fellows Symposium, UIUC, 2014


2014

Chinese Government Award for Outstanding Self-Financed Students Abroad, 2014

Baxter International Young Investigator Award, 2017

25. Sherrie Lu

Glenn E. and Barbara Ullyot Award, 2013

26. Zachary Dookeran

Dr. Joseph and Donna Glas in Memory of Professor James Westwater Award, 2013

27. Todd Freestone

First Place Award for Poster Presentation, 12th GSAC Research Symposium, Urbana,

IL, 2013

28. Kate Burton

Peter and Gretchen Markunas Memorial Scholarship, 2014

29. Robert Pipal

MCB Summer Research Fellowship, 2014

30. Stella Wu

MCB Summer Research Fellowship, 2014

Highest Distinction for Senior Thesis, 2014

31. Run Jin

Bronze Tablet, UIUC, 2014

32. Ran Chao

3M Graduate Fellowship, 2014-2015


33. Zhanar Abil

Graduate College Travel Award, UIUC, 2014

AIChE FPBE Division Student Poster Award, 2014

34. Zehua Bao

CMB Travel Award, UIUC, 2015


35. Yuhao Min

John E. Gieseking Scholarship, 2015

Carl Woese Institute for Genomic Biology Undergraduate Research Scholar, 2017

Bronze Tablet University Honor, 2019

36. Yajie Wang

Dow Chemical Fellowship, 2015

53

3M Graduate Fellowship, 2016


UIUC, 2017

Chia-chen Chu Fellowship, 2018

Glenn E. and Barbara R. Ullyot Graduate Fellowship, 2018

37. Behnam Enghiad



Hanratty Travel Award, 2018

38. Xiong Xiong


UIUC, 2015

39. Ipek Tasan

First place Award, Graduate College Research Live!, UIUC, 2015

Biochemistry Travel Grant, 2018

40. Wen Ren

Outstanding Oral Presentation in Science & Math, UIUC Undergraduate Research

Symposium, 2016

41. Lingyang Ju

Highest Distinction for Senior Thesis, 2016

42. Hengqian Ren


UIUC, 2016

40. Sam Hamedi


UIUC, 2016

40. Justin Genova

Chester W. Hannum Scholarship, Department of Chemical and Biomolecular

Engineering, UIUC, 2017

41. Yuying Wu

Chester W. Hannum Scholarship, Department of Chemical and Biomolecular


41. Scott Weisberg

MCB Summer Research Award, UIUC, 2017

42. Sherri Ho

MCB Summer Research Award, UIUC, 2017

43. Surbhi Jain

MCB Excellent TA award, UIUC, 2017

Biochemistry Travel Grant, UIUC, 2018

44. Mason Xue

Graduate College Conference Travel Award, 2018

IGB Fellows’ Symposium Best Poster Award, 2018

First Place Poster award in the CABBI Annual Retreat, 2019

45. John Carl Schultz

54


UIUC, 2019

Dupont Fellowship, 2019

46. Linzixuan Zhang


UIUC, 2019

Chester W. Hannum Chemical Engineering Scholarship, Department of Chemical and

Biomolecular Engineering, UIUC, 2019

47. William Lyon

Clarence G. Gerhold Memorial Scholarship, Department of Chemical and Biomolecular


Barry Goldwater Scholarship, Goldwater Foundation, 2020

48. Meng Zhang

Mavis Future Faculty Fellow, 2020

49. Andrea Mejia

R.J Van Mynen Chemical Engineering Scholarship, Department of Chemical and

Biomolecular Engineering, UIUC, 2020

(4) Unpublished Conference Paper Presentations (presenting author underlined)

1. H. Zhao, Y. Li, and F.H. Arnold. “Strategies for Directed Evolution of a Peptide Ligase.”

XIII International Enzyme Engineering Conference, San Diego, CA, October 15-20, 1995.

2. H. Zhao and F.H. Arnold. “Functional and Non-functional Mutations Distinguished by

Random Recombination of Homologous Genes.” Proteins Gordon Research Conference,

Holderness, New Hampshire, June 15-20, 1997.

3. H. Zhao and F.H. Arnold. “Directed Evolution of New Biocatalysts: Subtilisin E as a Test

Case.” AIChE Annual Meeting, Los Angeles, CA, November 12, 1997.

4. H. Zhao. “The Development of a Dehalogenase-based Process for the Production of

Commodity Chemicals.” AIChE Annual Meeting, Dallas, TX, November 18, 1999.

5. H. Zhao. “Directed Evolution of Highly Active Antifreeze Proteins.” AIChE Annual

Meeting, Reno, NV, November 5, 2001.

6. Z. Chen and H. Zhao. “Functional Studies of Human Estrogen Receptor via Directed

Evolution.” AIChE Annual Meeting, Indianapolis, IN, November 5, 2002.

7. K. Chockalingam and H. Zhao. “Directed Evolution of Human Manganese Superoxide

Dismutase.” AIChE Annual Meeting, Indianapolis, IN, November 7, 2002.

8. Z. Chen and H. Zhao. “Functional Studies of Human Estrogen Receptor via Directed

Evolution.” Illinois Biophysics Society Student Symposium, Urbana, IL, March 12, 2003.

9. R. Woodyer, W. A. van der Donk, and H. Zhao. “Relaxing the Nicotinamide Cofactor

Specificity of Phosphate Dehydrogenase by Rational Design.” ACS PRF Chemical Biology

Symposium. Ithaca, NY, July 7, 2003.

10. O. Esteban and H. Zhao. “Directed Evolution of Functional Single-Chain HLA-DR1

Molecules.” The Protein Society 17th Annual Symposium, Boston, MA, July 27, 2003.

11. R. Woodyer, W. A. van der Donk, and H. Zhao. “Relaxing the Nicotinamide Cofactor

Specificity of Phosphite Dehydrogenase by Rational Design: Development of a NAD(P)H

Regeneration Catalyst.” UIUC Biochemistry Fall Research Conference, Urbana, IL,

September 19, 2003.

55

12. Z. Chen, K. Chockalingam, and H. Zhao. “Directed Evolution of Human Estrogen

Receptor for Fun and Profits.” AIChE Annual Meeting, San Francisco, CA, November 20,

2003.

13. W. Zha and H. Zhao. “Rational Pathway Engineering of Type I Fatty Acid Synthase

Allows Biosynthesis of Triacetic Acid Lactone from D-Glucose in vivo.” ACS Annual

Meeting, Anaheim, CA. April 1, 2004.

14. O. Esteban and H. Zhao. “Directed Evolution of Soluble Single-chain Human Class II

MHC Molecules.” Symposium on the Evolution of Biomolecular Structure, Michigan State

University, East Lansing, MI, June 4, 2004.

15. W. Zha, Z. Shao, Z. Simurdiak, J. Lee, and H. Zhao.”Biosynthesis of Thermally Stable

Energetic Compounds via Pathway Engineering.” Metabolic Engineering V, Lake Tahoe,

CA, September 19-23, 2004.

16. K. Chockalingam and H. Zhao. “Engineering Genetic Switches for Biomedical

Applications.” UIUC ChBE Graduate Student Symposium, Urbana, IL, October 7, 2004.

17. W. Zha and H. Zhao. “Rational Pathway Engineering of a Type I Fatty Acid Synthase for

Biosynthesis of TAL in vivo.” UIUC ChBE Graduate Student Symposium, Urbana, IL,

October 7, 2004.

18. Z. Chen and H. Zhao. “Rapid Creation of Novel Protein Function by in vitro Coevolution.”

The Seventeenth Annual CMB/MB Research Symposium, Urbana, IL, November 4, 2004.

19. T.W. Johannes, R. Woodyer, and H. Zhao. “Directed Evolution of a Thermostable

Phosphite Dehydrogenase.” The Seventeenth Annual CMB/MB Research Symposium,

Urbana, IL, November 4, 2004.

20. Z. Shao and H. Zhao. “In vivo Biosynthesis of Triacetic Acid Lactone from D-Glucose by

Rational Design and Directed Evolution.” AIChE Annual Meeting, Austin, TX, November

10, 2004.

21. O. Esteban and H. Zhao. “Engineering Soluble Single-chain Human Class II MHC

Molecules.” AIChE Annual Meeting, Austin, TX, November 12, 2004.

22. E. Ang, J. Obbard, and H. Zhao. “Engineering an Enzymatic Carbazole Denitrogenation

Pathway.” ACS Annual Meeting, San Diego, March 13, 2005.

23. E. Ang, Z. Chen, and H. Zhao. “Towards Engineering of an Androgen Receptor Equivalent

from the Human Estrogen Receptor .” ACS Annual Meeting, San Diego, March 17, 2005.

24. R. Woodyer, T.W. Johannes, W.A. van der Donk, and H. Zhao. “Protein Engineering of

Phosphite Dehydrogenase for NAD(P)H Regeneration.” ACS Annual Meeting, San Diego,

March 16, 2005.

25. K. Chockalingam, Z. Chen, and H. Zhao. “Directed Evolution of Human Estrogen

Receptor.” Biochemical Engineering XIV, Harrison Hot Springs, British Columbia,

Canada, July 10, 2005 (poster).

26. R. Woodyer, W. A. van der Donk, and H. Zhao. “Directed Evolution and Application of

Phosphite Dehydrogenase.” 1st Annual Chemistry-Biology Interface Training Program

Symposium, University of Illinois, Urbana, August 26, 2005.

27. T.W. Johannes, R. Woodyer, and H. Zhao. “Development of Phosphite Dehydrogenase-

based NAD(P)H Regeneration System.” UIUC ChBE Graduate Student Symposium,

Urbana, IL, October 6, 2005.

56

28. K. Chockalingam, Z. Chen, and H. Zhao. “Systematic Approaches to the Protein

Engineering of Highly Specific Receptor-Ligand Pairs.” UIUC ChBE Graduate Student

Symposium, Urbana, IL, October 6, 2005 (poster presentation).

29. Z. Shao, T. Johannes, H. Zhao. “Biosynthesis of Phosphonic Acid Antibiotics in E. coli.”

UIUC ChBE Graduate Student Symposium, Urbana, IL, October 6, 2005 (poster

presentation).

30. W. Zha and H. Zhao. “Biosynthesis of Phloroglucinol.” UIUC ChBE Graduate Student


31. T. Johannes and H. Zhao. “Development of a Phosphite Dehydrogenase-Based

Nicotinamide Cofactor Regeneration System.” The Eighteenth Annual CMB/MB Research


32. Chen, Z. and H. Zhao. “Directed Evolution of Homing Endonuclease with Novel DNA

Sequence Specificity.” The Eighteenth Annual CMB/MB Research Symposium, Urbana,

IL, October 14, 2005 (poster presentation).

33. K. Chockalingam, Z. Chen, and H. Zhao. “Systematic Approaches to the Protein

Engineering of Highly Specific Receptor-Ligand Pairs.” The Eighteenth Annual CMB/MB

Research Symposium, Urbana, IL, October 14, 2005.

34. Z. Chen and H. Zhao. “Directed Evolution of Proteins for Biomedical Application.”

AIChE Annual Meeting, Cincinnati, OH, October 30, 2005 (poster presentation).

35. K. Chockalingam and H. Zhao. “Systematic Approaches to the Protein Engineering of

Highly Specific Receptor-Ligand Pairs.” AIChE Annual Meeting, Cincinnati, OH, October

30, 2005.

36. Z. Chen and H. Zhao. “Directed Evolution of Homing Endonuclease with Altered DNA

Sequence Specificity.” AIChE Annual Meeting, Cincinnati, OH, October 31, 2005.

37. Z. Chen and H. Zhao. “Directed Evolution of Homing Endonuclease with Novel DNA

Sequence Specificity.” AIChE Annual Meeting, Cincinnati, OH, November 2, 2005 (poster

presentation).

38. T.W. Johannes and H. Zhao. “Development of a Phosphite Dehydrogenase-Based

Nicotinamide Cofactor Regeneration System.” AIChE Annual Meeting, Cincinnati, OH,

November 3, 2005.

39. K. Chockalingam, Chen, Z. and H. Zhao. “Directed Evolution of Specific Receptor-Ligand

Pairs for Use in the Creation of Gene Switches.” AIChE Annual Meeting, Cincinnati, OH,

November 3, 2005.

40. T. Johannes and H. Zhao, “Development of a Novel Phosphite Dehydrogenase-Based

NAD(P)H Regeneration System for Industrial Biocatalysis.” Graduate Seminar in the

Applied Chemical Sciences, Urbana, IL, May 2006.

41. H. Zhao, R. Woodyer, T.W. Johannes, and M.J. Mclachlan. “Development of a novel

phosphite dehydrogenase based NAD(P)H regeneration system for industrial biocatalysis.”

ACS Annual Meeting, San Francisco, CA, September 10, 2006.

42. H. Zhao, K. Chockalingam, M. Mclachlan, and K. Lai. “Protein engineering of gene

switches.” ACS Annual Meeting, San Francisco, CA, September 13, 2006.

43. H. Zhao, Z. Chen, Y. Choi, and S. Rubin-Pitel. “Directed evolution of homing

endonuclease for gene targeting.” ACS Annual Meeting, San Francisco, CA, September 14,

2006.

57

44. E. Ang, J. Obbard, and H. Zhao. “Engineering of a Carbazole Denitrogenation Pathway

through Directed Evolution.” AIChE Annual Meeting, San Francisco, CA, November 14,

2006.

45. F. Wen and H. Zhao. “CD4+ T-Cell Epitope Identification Using Yeast Displaying Single

Chain Class II MHC Molecules as Artificial APCs.” The 5th Annual UIUC ChBE Graduate

Student Symposium, Urbana, IL, October, 2006 (poster presentation).

46. W. Zha and H. Zhao. “Biosynthesis of Phloroglucinol via Metabolic Pathway

Engineering.” The 5th Annual UIUC ChBE Graduate Student Symposium, Urbana, IL,

October, 2006.

47. T.W. Johannes and H. Zhao. “Development of a Phosphite Dehydrogenase-Based

Nicotinamide Cofactor Regeneration System.” The 5th Annual UIUC ChBE Graduate

Student Symposium, Urbana, IL, October, 2006.

48. F. Wen and H. Zhao. “CD4+ T-Cell Epitope Identification Using Yeast Displaying Single

Chain Class II MHC Molecules as Artificial APCs.” AIChE Annual Meeting, San

Francisco, CA, November 14, 2006.

49. Z. Shao, R. Woodyer, and H. Zhao. “Biosynthesis of Fosfomycin.” AIChE Annual

Meeting, San Francisco, CA, November 16, 2006.

50. N.U. Nair and H. Zhao. “Evolution in Reverse: Engineering a Xylose-Specific Xylose

Reductase.” AIChE Annual Meeting, San Francisco, CA, November 17, 2006.

51. N. Nair and H. Zhao. “Evolution in Reverse: Engineering a Xylose-Specific Xylose

Reductase.” ACS Annual Meeting, Boston, MA, August 19, 2007.

52. Z. Shao, R. Woodyer, and H. Zhao. “Biosynthesis of Fosfomycin.” The 6th Annual UIUC

ChBE Graduate Student Symposium, Urbana, IL, October, 2007.

53. N. Nair and H. Zhao. “Evolution in Reverse: Engineering a Xylose-Specific Xylose

Reductase.” The 6th Annual UIUC ChBE Graduate Student Symposium, Urbana, IL,

October, 2007 (poster presentation).

54. S. Rubin-Pitel, W. Zha, and H. Zhao. “Protein and Metabolic Engineering to Enhance

Phloroglucinol Biosynthesis.” The Tenth Annual CMB/MB Research Symposium, Urbana,

IL, October, 2007.

55. T.W. Johannes and H. Zhao. “Heterologous Production of the Antimalarial Drug FR-

900098 in E. coli.” AIChE Annual Meeting, Salt Lake City, UT, November, 2007.

56. M.J. McLachlan and H. Zhao. “Development of Gene Switches by Protein Engineering.”

AIChE Annual Meeting, Salt Lake City, UT, November, 2007.

57. M.J. McLachlan and H. Zhao. “Directed Evolution of Orthogonal Ligand Specificity in a

Single Scaffold.” The 6th Annual Biophysics and Computational Biology Symposium,

Urbana, IL, June 6, 2008.

58. Z. Shao, H. Zhao, and H. Zhao. “DNA Assembler, a Highly Efficient Approach for Rapid

Construction of Large Recombinant DNA for Metabolic Pathway Engineering and

Synthetic Biology.” ACS Annual Meeting, Philadelphia, PA, August 19, 2008.

59. R.P. Sullivan and H. Zhao. “Engineering a Fungal L-Arabinose Pathway towards the

Utilization of Pentose Sugars for Production of Xylitol and Ethanol.” The 7th Annual UIUC


60. S.B. Rubin-Pitel, H. Zhang, J. Brunzelle, T. Vu, H. Zhao and S. Nair. “Structure-function

Analysis of a Type III Polyketide Synthase from Neurospora crassa.” The 7th Annual

UIUC ChBE Graduate Research Symposium, Urbana, Illinois, October, 2008 (poster).

58

61. N.U. Nair, Z. Shao, H. Zhao, T.H. Lee, R.P. Sullivan, M.J. McLachlan, T.W. Johannes,

and H. Zhao. “Biobutanol from Yeast. A Synergistic Genome and Protein Engineering

Approach.” The 7th Annual UIUC ChBE Graduate Student Symposium, Urbana, IL,

October, 2008.

62. F. Wen and H. Zhao. “Rapid Identification of CD4+ T cell Epitopes Using Yeast

Displaying Pathogen-derived Peptide Libraries.” The 7th Annual UIUC ChBE Graduate


63. Z. Shao, H. Zhao, and H. Zhao. “DNA Assembler, an in vivo Genetic Method for Rapid

Construction of Large Recombinant DNA.” The 7th Annual UIUC ChBE Graduate Student

Symposium, Urbana, IL, October, 2008 (poster).

64. S.B. Rubin-Pitel, W. Zha, and H. Zhao. “Structure-function Analysis of a Type III

Polyketide Synthase from Neurospora crassa.” The Eleventh Annual CMB/MB Research

Symposium, Urbana, IL, November, 2008 (poster).

65. N.U. Nair, Z. Shao, H. Zhao, T.H. Lee R.P. Sullivan, M.J. McLachlan, T.W. Johannes, and

H. Zhao. “Biobutanol from yeast. A Synergistic Genome and Protein Engineering

Approach.” The Eleventh Annual CMB/MB Research Symposium, Urbana, IL, November,

2008.

66. F. Wen and H. Zhao. “Rapid Identification of CD4+ T cell Epitopes Using Yeast

Displaying pathogen-derived peptide libraries.” The Eleventh Annual CMB/MB Research

Symposium, Urbana, IL, November, 2008 (poster).

67. R.P. Sullivan, N.U. Nair, and H. Zhao. “Engineering a fungal L-arabinose pathway towards

the utilization of pentose sugars for production of xylitol and ethanol.” AIChE Annual

Meeting, Philadelphia, DE, November, 2008.

68. N.U. Nair, Z. Shao, H. Zhao, R. P. Sullivan, M. McLachlan, T.W. Johannes, and H. Zhao.

“Biobutanol from yeast. A synergistic genome and protein engineering approach.” AIChE

Annual Meeting, Philadelphia, DE, November, 2008.

69. S.B. Rubin-Pitel, W. Zha, and H. Zhao. “Directed evolution of a phloroglucinol producing

Type III polyketide synthase.” AIChE Annual Meeting, Philadelphia, DE, November,

2008.

70. M.A. DeSieno, T.W. Johannes, and H. Zhao. “Deciphering the late steps in FR900098

biosynthesis.” AIChE Annual Meeting, Philadelphia, DE, November, 2008.

71. J. Du, S. Li, and H. Zhao. “Cloning and characterization of novel pentose-specific

transporters.” ACS Annual Meeting, Washington DC, August, 2009

72. D. Eriksen, R. Sullivan, and H. Zhao. “Toward the development of a cost-effective process

for xylitol production.” ACS Annual Meeting, Washington DC, August, 2009 (poster)

73. W. Tang, Z. Li, and H. Zhao. “Directed evolution of an enantioselective P450pyr for the

preparation of chiral pharmaceutical intermediates.” ACS Annual Meeting, Washington

DC, August, 2009

74. N. Nair and H. Zhao. “Selective production of xylitol from hemicellulosic sugars using a

combined protein and metabolic engineering approach.” ACS Annual Meeting,

Washington DC, August, 2009

75. R. Cobb, M. A. DeSieno, and H. Zhao. “Directed evolution of the biosynthetic enzyme

FrbF for combinatorial biosynthesis of FR-900098.” ACS Annual Meeting, Washington

DC, August, 2009 (poster)

59

76. N. Sun and H. Zhao. “Directed evolution of homing endonucleases for gene targeting.”

UIUC Biochemistry Fall Research Conference, Urbana, IL, September, 2009.

77. R. P. Sullivan and H. Zhao. “Microbial synthesis of xylitol from hemicellulosic sugars.”

Regional AIChE meeting, Chicago, IL, October, 2009.

78. T. H. Lee, R. P. Sullivan, J. Du, and H. Zhao. “Engineering an L-arabinose/xylose co-

utilizing S. cerevisiae for biofuels production.” Regional AIChE meeting, Chicago, IL,

October, 2009.

79. F. Wen, J. Sun, and H. Zhao. “Recombinant yeast strains towards consolidated

bioprecessing: surface display of functional mini-cellulosomes for direct conversion of

cellulose to ethanol.” Regional AIChE meeting, Chicago, IL, October, 2009.

80. N. Nair and H. Zhao. “Production of xylitol from hemicellulosic sugars using a combined

protein and metabolic engineering approach.” The 8th Annual UIUC ChBE Graduate


81. F. Wen, Jie Sun, and H. Zhao. “Development of recombinant yeast strains displaying

minicellulosomes for synergistic saccharification and direct fermentation of cellulose to

ethanol.” The 8th Annual UIUC ChBE Graduate Student Symposium, Urbana, IL, October,

2009.

82. C. Denard, J. F. Hartwig, and H. Zhao. “Directed evolution of metalloenzymes for selective

catalysis: Tandem isomerization and epoxidation of alkene mixtures from alkane

dehydrogenation.” The 8th Annual UIUC ChBE Graduate Student Symposium, Urbana, IL,

October, 2009 (poster).


for xylitol production.” The 12th Annual CMB/MB Research Symposium, Urbana, IL,



FrbF for combinatorial biosynthesis of FR-900098.” The 12th Annual CMB/MB Research


85. J. Liang, M. McLachlan, and H. Zhao. “Engineering a novel orthogonal gene switch for

human therapeutics.” The 12th Annual CMB/MB Research Symposium, Urbana, IL,


86. F. Wen, J. Sun, and H. Zhao. “Yeast surface display of trifunctional minicellulosomes for

simultaneous saccharification and fermentation of cellulose to ethanol .” The 12th Annual

CMB/MB Research Symposium, Urbana, IL, October, 2009 (poster).

87. Y. Luo, Z. Shao, and H. Zhao. “Uncovering cryptic biosynthetic pathways using synthetic

biology.” The 12th Annual CMB/MB Research Symposium, Urbana, IL, October, 2009.

88. N.U. Nair, T. Lee, Z. Shao, H. Zhao, R. P. Sullivan, M. McLachlan, T.W. Johannes, and H.

Zhao. “Biobutanol from yeast. A synergistic genome and protein engineering approach.”

AIChE Annual Meeting, Nashville, TN, November, 2009.

89. A. Ghosh, J. Du, B. Kim, R. P. Sullivan, H. Zhao, and N. D. Price. “In silico and

experimental studies of cofactor balance in the engineered pentose sugar utilization

pathways in Saccharomyces cerevisiae.” AIChE Annual Meeting, Nashville, TN,

November, 2009.

90. Z. Shao, Y. Luo, and H. Zhao. “Applying the “DNA Assembler” approach to study natural

product biosynthetic gene clusters.” AIChE Annual Meeting, Nashville, TN, November,

2009.

60

91. F. Wen, O. Esteban, and H. Zhao. “Direct epitope mapping of T cells from influenza A

virus genome using yeasts displaying MHC-peptide complexes as artificial antigen

presenting cells.” AIChE Annual Meeting, Nashville, TN, November, 2009.

92. F. Wen, J. Sun, and H. Zhao. “Recombinant yeast strains towards consolidated

bioprecessing: surface display of functional mini-cellulosomes for direct conversion of

cellulose to ethanol.” AIChE Annual Meeting, Nashville, TN, November, 2009.

93. N.U. Nair and H. Zhao. “Selective production of xylitol from hemicellulosic sugars - using

a combined protein and metabolic engineering approach.” AIChE Annual Meeting,

Nashville, TN, November, 2009.

94. M. McLachlan and H. Zhao. “Development of a mammalian cell-based high throughput

screening method for the directed evolution of genetic switches.” AIChE Annual Meeting,

Nashville, TN, November, 2009.

95. M. A. DeSieno and H. Zhao. “Biosynthesis of the antimalarial drug FR-900098 in E. coli.”

AIChE Annual Meeting, Nashville, TN, November, 2009.


for xylitol production.” Bioengineering @ Illinois Day Graduate Student Poster

Competition, Urbana, IL, April 2010 (poster).


FrbF for combinatorial biosynthesis of FR-900098.” Bioengineering @ Illinois Day

Graduate Student Poster Competition, Urbana, IL, April 2010 (poster).


biology.” Bioengineering @ Illinois Day Graduate Student Poster Competition, Urbana, IL,

April 2010 (poster).

99. J. Liang, M. McLachlan, and H. Zhao. “Engineering a novel orthogonal gene switch for

human therapeutics.” Bioengineering @ Illinois Day Graduate Student Poster

Competition, Urbana, IL, April 2010 (poster).


biology.” The 9th Annual UIUC ChBE Graduate Student Symposium, Urbana, IL, October,

2010 (poster).

101. Z. Shao, Y. Luo, and H. Zhao. “Rapid characterization and engineering of natural product

biosynthetic pathways via DNA assembler.” The 13th Annual CMB/MB Research

Symposium, Urbana, IL, October, 2010.

102. M.A. DeSieno, H. Zhao. “Biosynthesis of Phosphonates for the Treatment of Malaria.” The

9th Annual UIUC ChBE Graduate Student Symposium, Urbana, IL, October, 2010 (poster).

103. C. Denard, J. F. Hartwig, and H. Zhao. “Marrying organometallic catalysts with

metalloenzymes: tandem isomerization and epoxidation of olefins with ruthenium catalysts

and P450 enzymes.” The 13th Annual CMB/MB Research Symposium, Urbana, IL,


104. R. Cobb, M.A. DeSieno, H. Zhao. “Characterization and directed evolution of FrbF for

combinatorial biosynthesis of FR-900098.” The 13th Annual CMB/MB Research


105. J. Du and H. Zhao. “Metabolic engineering of Saccharomyces cerevisiae towards efficient

ethanol production from pentose sugars.” The 9th Annual UIUC ChBE Graduate Student


61

106. S. Li, J. Du, and H. Zhao. “Overcoming glucose repression in mixed sugar fermentation by

co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae.”

The 9th Annual UIUC ChBE Graduate Student Symposium, Urbana, IL, October, 2010.

107. C. Denard, R. Giri, M. Jarenmark, J. F. Hartwig & H. Zhao. “Biphasic Tandem Catalysis:

Isomerization and Epoxidation of Olefins with Ruthenium Catalysts and P450 Enzymes.”

NSF CENTC annual meeting, Seattle, Washington, September 13, 2010.

108. J. Du and H. Zhao. “Directed Evolution of a Highly Efficient Arabinose/Xylose Utilization

Pathway in Saccharomyces cerevisiae.” AIChE Annual Meeting, Salt Lake City, UT,

November, 2010.

109. M. A. DeSieno and H. Zhao. “Biosynthesis of Phosphonates for the Treatment of Malaria.”

AIChE Annual Meeting, Salt Lake City, UT, November, 2010.

110. A. Ghosh, H. Zhao, and N. Price. “Integrated Probabilistic Regulatory and Metabolic

Network Analysis of Saccharomyces cerevisiae for Biochemical Production.” AIChE

Annual Meeting, Salt Lake City, UT, November, 2010.

111. S. Li, J. Du, J. Sun, J. M. Galazka, N. L. Glass, J. H. D. Cate, X. Yang, and H. Zhao.

“Overcoming Glucose Repression in Mixed Sugar Fermentation by Co-expressing a

Cellobiose Transporter and a β-glucosidase in Saccharomyces cerevisiae.” AIChE Annual

Meeting, Salt Lake City, UT, November, 2010.

112. R. E. Cobb, M. A. DeSieno, and H. Zhao. “Characterization and Directed Evolution of

FrbF for Combinatorial Biosynthesis of FR-900098.” ACS Annual Meeting, Anaheim, CA,

March, 2011.


Biosynthetic Pathways via DNA Assembler.” ACS Annual Meeting, Anaheim, CA, March,

2011.

114. S. Li and H. Zhao. “New Strategies to Overcome Glucose Repression in Mixed Sugar

Fermentation in Saccharomyces cerevisiae” 33rd Symposium on Biotechnology for Fuels

and Chemicals, Seattle, WA, May, 2011.


FrbF for Combinatorial Biosynthesis of Novel FR-900098 Derivatives.” Society for

Industrial Microbiology Annual Meeting, New Orleans, LA, July, 2011.

116. N. Sun, J. Liang, Z. Abil, and H. Zhao. “Protein Design of TAL Effector Nucleases

(TALENs) as a New Genome Engineering Tool.” Enzyme Engineering XXI (An ECI

Conference Series), Vail, CO, September, 2011.

117. J. Du, B. Kim, and H. Zhao. “Customized Optimization of Metabolic Pathways by

Combinatorial Transcriptional Engineering (COMPACTER).” AIChE Annual meeting,

Minneapolis, MN, October, 2011.

118. J. Du and H. Zhao. “Engineering Saccharomyces cerevisiae for Biofuel Production from

Lignocellulosic Biomass.” AIChE Annual meeting, Minneapolis, MN, October, 2011.

119. B. Kim and H. Zhao. “Metabolic Flux Analysis of the Central Metabolism of Laboratory

and Industrial Saccharomyces cerevisiae Strains Using 13C-Labeling Experiments.” AIChE

Annual Meeting, Minneapolis, MN, October, 2011.

120. J. Liang, M. J. McLachlan, and H. Zhao. “Using an Orthogonal Ligand-receptor Pair to

Control Endogenous Gene Expression in Mammalian Cell.” AIChE Annual Meeting,

Minneapolis, MN, October, 2011

121. Y. Luo, Z. Shao, and H. Zhao. “Uncovering Cryptic Biosynthetic Pathways Using

62

Synthetic Biology”. AIChE Annual Meeting, Minneapolis City, MN, October, 2011.

122. Z. Shao and H. Zhao. “Refactoring Biosynthetic Pathways via Synthetic Biology.” AIChE

Annual Meeting, Minneapolis, MN, October, 2011.

123. Z. Shao, Z. Abil, C. Li, Y. Luo, T. Freestone, and H. Zhao. “Refactoring Biosynthetic

Pathways: Delineating Pathway Expression from Sophisticated Regulation Cascades via

Synthetic Biology.” AIChE Annual Meeting, Minneapolis, MN, October, 2011.


Biosynthetic Pathways via DNA Assembler.” AIChE Annual Meeting, Minneapolis, MN,

October, 2011.


FrbF for the Biosynthesis of Novel Antimalarial Compounds.” The 10th Annual UIUC


126. N. Sun, J. Liang, Z. Abil, and H. Zhao. “Engineered TAL Effector Nucleases (TALENs)

for Use in Treatment of Sickle Cell Disease.” The 24th Annual CMB-MB Research


127. N. Sun, J. Liang, Z. Abil, and H. Zhao. “Optimized TAL Effector Nucleases (TALENs) for

Use in Treatment of Genetic Disease”. The 10th Annual UIUC ChBE Graduate Student


128. Z. Abil, N. Sun, J. Liang, and H. Zhao. “Engineering TAL Effector Nucleases for

Targeting the ∆F508 Mutation in CFTR.” The 10th ChBE Graduate Research Symposium,

Urbana, IL, October, 2011.

129. J. Liang, M. J. McLachlan, and H. Zhao. “Using an Orthogonal Ligand-receptor Pair to

Control Endogenous Gene Expression in Mammalian Cell.” Cell and Molecular Biology &

Molecular Biophysics Training Grants 24th Annual Research Symposium, Urbana, IL,

October, 2011.

130. Y. Luo, Z. Shao, and H. Zhao. “Uncovering Cryptic Biosynthetic Pathways Using

Synthetic Biology.” The 24nd Cell and Molecular Biology & Molecular Biophysics

Training Grants (CMB/MBTG) Annual Symposium, Urbana, IL, October, 2011.

131. E. Chanco, M. Simurdiak, Y. Choi, N. Sun, and H. Zhao. “Characterization of the N-

Oxygenase AurF from Streptomyces thioletus.” Molecular and Cell Biology & Molecular

Biophysics Training Grant Symposium, Urbana, IL, October, 2011.

132. Z. Abil, N. Sun, J. Liang, and H. Zhao. “Engineering TAL Effector Nucleases for

Targeting the ∆F508 Mutation in CFTR.” The 24th Annual CMBTG Research Symposium,

Urbana, IL, October, 2011.

133. S. Li and H. Zhao. “New Strategies to Overcome Glucose Repression in Mixed Sugar

Fermentation in Saccharomyces cerevisiae” 3rd Pan American Congress on Plants and

Bioenergy, Urbana, IL, July 16, 2012 (poster).

134. D. T. Eriksen, B. Kim, Y. Yuan, J. Du, H.Zhao. “Pathway Engineering for Efficient C5/C6

Utilization via Combinatorial and Evolutionary Strategies.” 3rd Pan American Congress on

Plants and Bioenergy. Urbana, IL. July, 2012 (poster)

135. D.T. Eriksen, D. Coursolle, S. Li, J. Lian, X. Feng, R. Chao, M. Wang. H. Zhao.

“Engineering Efficient C5/C6 Sugar Utilization into Advanced Biofuels via

Saccharomyces cerevisiae.” 3rd Pan American Congress on Plants and Bioenergy, Urbana,

IL, July, 2012 (poster)

63

136. C. Denard, H. Huang, L. Lu, Y. Tan, J. F. Hartwig and H. Zhao. "Engineering Novel

Tandem Catalytic Reactions Using Organometallic Catalysts and Metalloenzymes".

CENTC annual meeting, Seattle, WA, September, 2012 (poster)

137. H. Huang, C. Denard, L. Lu, A. Crisci, S. Scott, J.A. Dumesic, H. Zhao. “Engineering of

Xylose Isomerase for HMF Production.” 2012 CENTC Annual Meeting, Seattle,

Washington, September, 2012 (poster)

138. L. Lu, C. Denard, H. Huang, and H. Zhao. “Engineering Regenerative Systems for

Metathesis-Oxidation Tandem Reactions.” 2012 CENTC Annual Meeting, Seattle,

Washington. September, 2012 (poster)

139. N. Sun, J. Liang, Z. Abil, and H. Zhao. “Engineering TAL Effector Nucleases (TALENs)

for Targeted Genome Editing.” AIChE Annual Meeting, Pittsburgh, PA, October, 2012.

140. N. Sun and H. Zhao. “Targeted Genome Editing by TAL Effector Nucleases in Human

Stem Cells.” The 11th Annual UIUC ChBE Graduate Research Symposium, Urbana, IL,

October, 2012.

141. N. Sun, J. Liang, Z. Abil, and, H. Zhao. “Engineering TAL Effector Nucleases (TALENs)

for Targeted Genome Editing.” The 25th MBTG Annual Research Symposium, Urban, IL,

October, 2012.

142. R. E. Cobb and H. Zhao. “Biosynthesis and Discovery of Novel Phosphonic Acid

Compounds.” AIChE Annual Meeting, Pittsburgh, PA, October, 2012.

143. M. Wang, and H. Zhao. “Aryl-aldehydes in fungal polyketides: Discovery and

characterization of novel biosynthesis pathways.” AIChE Annual Meeting, Pittsburgh, PA,

October, 2012.

144. Y. Luo, and H. Zhao. “Apply a Synthetic Biology Approach to Demystify the Target

Cryptic Pathway for Novel Natural Product Discovery.” AIChE Annual Meeting,

Pittsburgh, PA, October, 2012.

145. Y. Luo and H. Zhao. “Apply a Synthetic Biology Approach to Demystify the Target

Cryptic Pathway for Novel Natural Product Discovery.” The 11th Annual UIUC ChBE

Graduate Research Symposium, Urbana, IL, October, 2012.

146. J. Liang, J. C. Ning, and H. Zhao. "Coordinated Induction of Multi-gene Pathways in

Saccharomyces cerevisiae." AIChE Annual Meeting, Pittsburgh, PA, October, 2012.

147. D.T. Eriksen, H. Zhao. “Directed Evolution of a Cellobiose Utilization Pathway in

Saccharomyces cerevisiae through Simultaneous Engineering of Multiple Proteins.”

AIChE Annual Meeting, Pittsburgh, PA, October, 2012.

148. C. Denard, H. Huang, L. Lu, Y. Tan, J.F. Hartwig and H. Zhao. "Engineering Novel

Tandem Catalytic Reactions Using Organometallic Catalysts and Metalloenzymes".

AICHE annual meeting, Pittsburgh, PA, October, 2012.

149. D.T. Eriksen, P.C.H. Hsieh, P. Lynn,H. Zhao, H. “Simultaneously Engineering Multiple

Proteins for Pathway–Scale Protein Engineering.” EBI Internal Seminar. Urbana, IL.

February, 2013.

150. D.T. Eriksen, P.C.H. Hsieh, P. Lynn,H. Zhao, H. “Pathway Engineering through

Simultaneous Directed Evolution of Multiple Biosynthetic Pathway Proteins.” IGB Fellows

Symposium. Urbana, IL. May, 2013.

151. T. Si, H. Zhao, “Eukaryotic Reprogramming by Directed Genome Evolution.” Engineering

Conference International, Biochemical and Molecular Engineering XVIII, Beijing, China,

June, 2013 (poster).

64

152. T. Si, H. Zhao, “Tools and Applications for Metabolic Engineering in Saccharomyces

cerevisiae.” Beijing Institute of Technology, Beijing, China, June, 2013.

153. Yunzi Luo, Carl Denard, Hua Huang, Zengyi Shao, John Hartwig, Huimin Zhao.

“Synthetic biology: Putting Synthesis into Biology.” IBC Applications for Enzyme

Technologies conference, San Francisco, CA, June 2013.

154. T. Si, H. Zhao, “Eukaryotic Reprogramming by RNAi-assisted Genome Evolution.”

Institute of Plant Physiology & Ecology, Shanghai Institute for Biology Sciences, Chinese

Academy of Sciences, Shanghai, China, July, 2013.

155. D.T. Eriksen, P.C.H. Hsieh, P. Lynn,H. Zhao, H. “Pathway Engineering through

Simultaneous Directed Evolution of Multiple Biosynthetic Pathway Proteins.” SIMB

Annual Meeting. San Diego, CA. August 2013.

156. D.T. Eriksen, P.C.H. Hsieh, P. Lynn,H. Zhao, H. “Optimizing a Cellobioise Utilization

Pathway by Simultaneously Engineering Multiple Proteins.” Chemical Engineering

Research Symposium. Urbana, IL. October 2013.

157. H. Huang, C. Denard, A.Crisci, R. Alamillo, S.Scott, J. A. Dumesic and H. Zhao. “One-pot

Enzyme and Solid Acid Catalysis to Convert Glucose to 5-Hydroxymethylfurfural.”

CENTC annual meeting, Seattle, WA, October, 2013.

158. T. Si, H. Zhao, "Turn on an endogenous pathway for 1-butanol production in

Saccharomyces cerevisiae." 12th Annual ChBE Graduate Symposium, Urbana, IL,

October, 2013.

159. T. Si, H. Zhao, "Eukaryotic Reprogramming by RNAi-Assisted Genome Evolution." 12th

Annual ChBE Graduate Symposium, Urbana, IL, USA, October, 2013.

160. T. Si, H. Zhao, “Fine-Tuning of Gene Expression in Saccharomyces cerevisiae Through

RNA Interference.” 2013 AlChE Annual Meeting, San Francisco, CA, USA, November,

2013.

161. T. Si, H. Zhao, “Eukaryotic Reprogramming by RNAi-assisted Genome Evolution.” 2013

AlChE Annual Meeting, San Francisco, CA, USA, November, 2013.

162. H. Xiao, Z. Shao, Y. Jiang, S. Dole and H. Zhao. “Exploiting Issatchenkia

orientalis SD108 as a new platform organism for organic acids production.” AICHE annual

meeting, San Francisco, CA, November, 2013.

163. X Feng, H Zhao. “Investigating Host Dependence of Xylose Utilization in Recombinant

Saccharomyces cerevisiae Strains Using RNA-Seq Analysis.” AIChE annual meeting, San

Francisco, CA, November, 2013.

164. X Feng, H Zhao. “13C Metabolic Flux Analysis of Xylose Metabolism in Saccharomyces

cerevisiae.” AIChE annual meeting, San Francisco, CA, November, 2013.

165. J. Liang, R. Chao, Z. Abil, Z. Bao and H. Zhao. “Fairytale: A High-Throughput TAL

Effector Synthesis Platform.” AIChE annual meeting, San Francisco, CA, November,

2013.

166. S. Jagtap, J.-K. Lee and H. Zhao. “Characterization and Engineering of a Novel Functional

Algal Polysaccharide Degrading Enzymatic Machinery for Biofuels Production.” AIChE

annual meeting, San Francisco, CA, November, 2013.

65

167. Z. Abil and H. Zhao. “Engineering PUF Proteins for Specific Recognition of Endogenous

RNA Sequences.” AICHE annual meeting, Pittsburgh, PA, November, 2013.

168. T. Si, H. Zhao, “RNAi-assisted Genome Evolution in Saccharomyces cerevisiae.”

Metabolic Engineering Research Laboratory, Agency for Science, Technology and

Research, Singapore, January, 2014.

169. T. Si, H. Zhao, “Eukaryotic Reprogramming by RNAi-assisted Genome Evolution.” 2014

IGB Fellows Symposium, Urbana, IL, USA, May, 2014

170. J. Lian, T. Si, N.U. Nair, H. Zhao. “Design and Construction of Acetyl-CoA

Overproducing Saccharomyces cerevisiae Strains.” 2014 IGB Fellows Symposium,

Urbana, IL, May, 2014 (poster)

171. J. Lian, R. Chao, H. Zhao. “Simultaneous Utilization of Glucose and Galactose to Produce

Enantiopure (2R, 3R)-Butanediol by an Engineered Saccharomyces cerevisiae Strain.”

Society for Industrial Microbiology and Biotechnology Annual Meeting, St. Louis, MO,

July, 2014 (poster)

172. J. Lian, R. Chao, H. Zhao. “Metabolic Engineering of a Saccharomyces cerevisiae Strain

Capable of Simultaneously Utilizing Glucose and Galactose to Produce Enantiopure

(2R,3R)-Butanediol.” First Joint Symposium on Systems and Synthetic Biotechnology,

Urbana, IL, August, 2014.

173. Y. Luo, H. Zhao. “Activation and characterization of cryptic natural products biosynthetic

pathways via synthetic biology.” Synthetic Biology of Antibiotic Production II, Sant Feliu,

Spain, August 30 - September 4, 2014.

174. Z. Bao, H. Xiao, H. Zhao. “Homology-Integrated CRISPR-Cas (HI-CRISPR) System for

One-Step Multigene Disruption inSaccharomyces cerevisiae.” The 13th Annual Chemical

and Biomolecular Engineering Graduate Research Symposium, Urbana, IL, October, 2014

(poster)

175. M. HamediRad, D. Eriksen, Y. Yuan, H. Zhao. "Improving Fatty Acid Ethyl Ester

Production by Heterologous Expression of Fatty Acid Synthase in Saccharomyces

cerevisiae." 2014 CHBE Graduate Research Symposium, Urbana, IL, October, 2014

(poster)

176. Z. Bao, H. Xiao, H. Zhao. “Customized CRISPR-Cas System for Multiple Simultaneous

Gene Disruptions in Saccharomyces cerevisiae.” AIChE Annual Meeting, Atlanta, GA,

November, 2014.

177. H. Xiao, H. Zhao. “Genome-wide RNAi screen reveals the E3 SUMO-protein ligase

gene SIZ1 as a novel determinant of furfural tolerance in S. cerevisiae.” AIChE annual

meeting, Atlanta, GA, November, 2014.

178. T. Si., H. Zhao, “Enabling Multiplex Genome Engineering in Yeast By RNAi and

CRISPR.” 2014 ChBE Graduate Symposium, Urbana, IL, USA, October 24, 2014.

179. T. Si, L. Zhang, R. Chao, H. Zhao, “Enabling Multiplex Genome Engineering in Yeast By

RNAi and CRISPR.” AIChE Annual Meeting, Atlanta, GA, November, 2014.

180. T. Si, Y. Luo, H. Xiao, H. Zhao, “Utilizing an Endogenous Pathway for 1-Butanol

Production in Saccharomyces cerevisiae.” AIChE Annual Meeting, Atlanta, GA,

November, 2014.

181. M. Wang, H. Zhao. “Design and Engineering of a Xylose Sensing-Regulation Gene

Circuit in Saccharomyces Cerevisiae: A Potential “Master Switch” for Efficient Xylose

Utilization.” AIChE Annual Meeting, Atlanta, GA, November, 2014.

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182. T. Freestone, H. Zhao. “Combinatorial pathway optimization and metabolic engineering for

increased production of the anti-malarial FR900098.” AIChE annual meeting, Atlanta, GA,

November, 2014.

183. Z. Abil, L. Cuculis, C.M. Schroeder, C. Hoogenraad, H. Zhao. “A Synthetic Device for

Intracellular mRNA Transport and Local Protein Translation in Eukaryotes.”

AIChE annual meeting, Atlanta, GA, November, 2014.

184. J. Lian, H. Zhao. “Precursor Metabolite Pool Engineering for Efficient Production of Fuels

and Chemicals in Saccharomyces cerevisiae.” Energy Biosciences Institute Internal

Seminar, Urbana, IL, November, 2014.

185. J. Lian, T. Si, N.U. Nair, H. Zhao. “Design and Construction of Acetyl-CoA

Overproducing Saccharomyces cerevisiae Strains.” AIChE annual meeting, Atlanta, GA,

November, 2014.

186. Y. Luo, H. Zhao. “Activation and characterization of cryptic natural products biosynthetic

pathways via synthetic biology.” AIChE Annual Meeting, Atlanta, GA, November, 2014.

187. Z. Abil, C. Hoogenraad, H. Zhao. “Engineering Chimeric RNA-Binding Proteins: Modular

Design, Novel Function.” AIChE annual meeting, Atlanta, GA, November, 2014 (poster).

188. Z. Bao, H. Xiao, M. HamediRad, R. Chao, J. Liang, H. Zhao. “Genome Scale Loss-of-

Function Screening in Saccharomyces cerevisiae Using CRISPR/Cas9 System.” The

14th Annual Chemical and Biomolecular Engineering Graduate Research Symposium,

Urbana, IL, October 23, 2015 (poster).

189. T. Si, R. Chao, H. Zhao, “Enabling Multiplex Genome Engineering in Yeast by RNAi and

CRISPR.” 2015 Synthetic Biology, Engineering, Evolution & Design Conference, Boston,

MA, USA, June 10-13, 2015 (poster).

190. Y. Wang, M.J. Bartlett, C.A. Denard, J.F. Hartwig, H. Zhao. “Exploring the Synergy

between Chemical Catalysis and Biocatalysis: Tandem Reactions Combining Biocatalysts

and Organometallic Complexes for Selective Synthesis.” CENTC Annual Meeting, Seattle,

2015 (poster).

191. Z. Bao, H. Xiao, M. HamediRad, R. Chao, J. Liang, H. Zhao. “Genome Scale Loss-of-

Function Screening in Saccharomyces cerevisiae Using CRISPR/Cas9 System.” AIChE

Annual Meeting, Salt Lake City, UT, November 8-13, 2015.

192. E. Garcia-Ruiz, E. Andersen, S. S Jagtap, H. Zhao. "Engineering a Bacillus subtilis Strain

Capable of Utilizing Marine Macroalgae for Biofuels Production." Genomic Science

Contractors–Grantees Meeting XIII USDA‐DOE Plant Feedstock Genomics for Bioenergy

Meeting. Washington, DC, February, 2015.

193. J. Lian, R. Jin, H. Zhao. "Induced Pathway Optimization by Antibiotic Doses

in Saccharomyces cerevisiae." AIChE Annual Meeting, Salt Lake City, UT, November 8-

13, 2015.

194. J. Lian, R. Jin, H. Zhao. "Pathway Optimization by Tuning Antibiotic Concentrations

in Saccharomyces cerevisiae." 14th Annual Graduate Research Symposium, Urbana, IL,

October 2015.

195. J. Ning, H. Zhao. “Discovery of Novel Strong Yeast Promoters for Refactoring Fungal

Natural Product Gene Clusters.” 2015 AIChE Annual Meeting, Salt Lake City, UT, USA,

November 8-13, 2015.

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196. T. Si, R. Chao, H. Zhao, “Automated Metabolic Engineering of Yeast Cells Using a

Biological Foundry.” 2015 AIChE Annual Meeting, Salt Lake City, UT, USA, November

8-13, 2015.

197. Y. Wang, M.J. Bartlett, C.A. Denard, J.F. Hartwig, H. Zhao. “Exploring the Synergy

between Chemical Catalysis and Biocatalysis: Tandem Reactions Combining Biocatalysts

and Organometallic Complexes for Selective Synthesis.” CENTC Annual Meeting, Seattle,

2015

198. X. Xiong, H. Zhao. “A Crispr-Cas9 Enabled Efficient and Scalable Method for High

Throughput Chip-Seq Analysis”, 2015 AIChE Annual Meeting, Salt Lake City, UT, USA,

November 8-13, 2015.

199. E. Ang, H. Zhao. “Developing of Microbial Cell Factories for Bio-based Chemicals.”

SynCTI Opening Ceremony and Symposium, September 30-October 1, 2015, Singapore.

200. H. Zhang, E. Ang, H. Zhao. “Production of adipic acid from sugar beet residue via

combined biological and chemical catalysis.” 2015 SIMB Annual Meeting and Exhibition,

PA (poster)

201. H. Sun, E. Ang, H. Zhao. “Directed evolution of a fluorinase for the biosynthesis of

organo-fluorine compounds.” Southeast Asia Catalysis Conference 2015, Singapore

(poster)

202. H. Zhang, E. Ang, H. Zhao. “Production of adipic acid from sugar beet residue via

combined biological and chemical catalysis.” Southeast Asia Catalysis Conference 2015,

Singapore (poster)

203. E. Ang, H. Zhao. “Engineering Cells for Sustainable Chemical Production.” Biosystems

Design 1.0 Symposium, February 9-10, 2015, Singapore.

204. S. Shi, E. Ang, H. Zhao. “Metabolic engineering of Saccharomyces cerevisiae for n-

butanol production.” Biosystems Design 1.0 Symposium, February 9-10, 2015, Singapore

(poster)

205. Z. Liu, E. Ang, H. Zhao. “Metabolic engineering of flavonoid production in

Saccharomyces cerevisiae.” Biosystems Design 1.0 Symposium, February 9-10, 2015,

Singapore (poster)

206. Y. Liang, E. Ang, H. Zhao. “An Engineered Penta-Functional Minicellulosome for

Simultaneous Saccharification and Ethanol Fermentation in Saccharomyces cerevisiae.”

Biosystems Design 1.0 Symposium, February 9-10, 2015, Singapore (poster)

III. RESIDENT INSTRUCTION

A. Supervision of Graduate Students

M.S. Thesis Students:

Former: 23

1. Karu Chockalingam, M.S., 2003, “Engineering a Gene Switch for Biomedical

Applications.”

2. Wenjuan Zha, M.S., 2004, “Pathway Engineering for Biosynthesis of Aromatic

Compounds in vivo.”

3. Tyler Johannes, M.S., 2005, “Development of a Phosphite Dehydrogenase Based

Nicotinamide Cofactor Regeneration System.”

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4. Zengyi Shao, M.S., 2005, “Pathway Engineering of Triacetic Acid Lactone Biosynthesis

and Fosfomycin Biosynthesis.”

5. Michael Simurdiak, M.S., 2005, “Cloning, Expression, Partial Characterization, and

Engineering of a Novel N-oxidase involved in Aureothin Biosynthesis.”

6. Sheryl Rubin-Pitel, M.S. 2006, “Investigation of an in vitro Enzymatic Method for the

Biosynthesis of Phloroglucinol.”

7. Ryan Sullivan, M.S. 2006, “Toward the Development of a Multi-Enzymatic Process for

Xylitol Synthesis from Biomass.”

8. Fei Wen, M.S. 2006, “Engineering of Yeast Displaying Single-chain MHC-Peptide

Complexes for Biomedical Applications.”

9. Nikhil Nair, M.S. 2006, “Investigation of Enzymatic Methods for Partially Purified

Hemicellulose Hydrolysate for Biosynthesis of Xylitol.”

10. Victor Gonzalez, M.S. 2008, “Directed Evolution of Novel Gene Switches.” joint student

with John Katzenellenbogen

11. Matt DeSieno, M.S. 2009, “Biosynthesis of the Antimalarial Drug FR-900098.”

12. Jing Du, M.S. 2010, “Discovery and Characterization of Pentose Specific Transporters in

Saccharomyces cerevisiae.”

13. Ryan Cobb, M.S. 2011, “Toward the Synthesis and Evaluation of Novel N-acylated FR-

900098 Analogs”

14. Yunzi Luo, M.S. 2011, “Discovering Novel Natural Products from Bioactive Plants and

Bacteria.”

15. Sijin Li, M.S. 2011, “Design of a Saccharomyces cerevisiae Strain Capable of

Simultaneously Utilizing Cellobiose and Xylose.”

16. Guodong Rao, M.S. 2012, “Directed Evolution of a Type III Polyketide Synthase and

Activation of Cryptic Phosphonic Acid Biosynthetic Pathways.”

17. Emmanuel Chanco, M.S., 2012. “Characterization and Reconstitution of Enzymes and

Pathways in Biosynthetic Production of Nitroaromatic Compounds.”

18. Dawn Eriksen, M.S. 2012. “Pathway Optimization and Engineering for Biofuel

Production.”

19. Carl Denard, M.S. 2012. “Engineering Novel Tandem Reactions Using Organometallic

Catalysts and Metalloenzymes.”

20. Jing Liang, M.S. 2013. “Gene Switches and Their Applications in Eukaryotic Systems.”

21. Tong Si, M.S. 2013. “An RNA Interference Based Platform for Genome Analysis and

Engineering in Saccharomyces cerevisiae.”

22. Jiazhang Lian, M.S. 2013. “Improving Advanced Biofuels Production in Saccharomyces

cerevisiae via Protein Engineering and Synthetic Biology Approaches.”

23. Jonathan Ning, M.S., 2014.

Ph.D. Thesis Students

Former: 32

1. Ryan Woodyer, Ph.D., August, 2005, “Understanding, Optimization, and Application of

Phosphite Dehydrogenase: Advancing NAD(P)H Regeneration.” Now research scientist

at Coke Cola, Atlanta, GA.

69

2. Karu Chockalingam, Ph.D., May, 2006, “Engineering Estrogen Receptor-based Gene

Switches and a Superoxide Dismutase for Therapeutic Applications.” Now process

development engineer at Kalon Biotherapeutics, College Station, TX.

3. Zhilei Chen, Ph.D., May, 2006, “Protein Engineering via in vitro Coevolution.” Now

associate professor at the Texas A & M University, College Station, TX.

4. Ee-Lui Ang, Ph.D., May, 2007, “Engineering of Aniline Dioxygenase for Bioremediation

and Industrial Applications.” Now principal investigator at the Institute for Chemical

Engineering and Science (ICES), Singapore.

5. Wenjuan Zha, Ph.D., September, 2007, “Protein and Metabolic Engineering for

Biosynthesis of Aromatic Compounds.” Research Assistant Professor, State University of

New York, Buffalo.

6. Tyler Johannes, Ph.D., April, 2008, “Directed Evolution of Phosphite Dehydrogenase

and Engineered Biosynthesis of FR-900098.” Now associate professor of chemical

engineering department at the University of Tulsa, Tulsa, OK.

7. Zengyi Shao, Ph.D., February 2009, “Enabling Natural Product Biosynthesis with Novel

Synthetic Biology Tools.” Now assistant professor at the Iowa State University.

8. Ryan Sullivan, Ph.D., November 2009, “Engineering a Fungal L-Arabinose Pathway

Towards the Co-utilization of Hemicellulosic Sugars for Production of Xylitol.” Now

research scientist at R&D Systems, Minneapolis, MN.

9. Sheryl Rubin, Ph.D., November 2009, “Type III Polyketide Synthases: Discovery,

Characterization, and Engineering.” Now research scientist at ExxonMobil, NJ.

10. Fei Wen, Ph.D., April 2010, “Cell Surface Display in Biomedical Applications and

Biofuels Production.” Now assistant professor at the chemical engineering department at

the University of Michigan.

11. Nikhil Nair, Ph.D., April 2010, “Synergy of Protein and Genome Engineering for Fuels

and Chemicals Production.” Now assistant professor at the chemical engineering

department at Tufts University.

12. Michael Mclachlan, Ph.D., August 2010, “Transcription Factor Engineering: Tools and

Applications.” Now research scientist at the Cellular Dynamics International, Inc.,

Madison, Wisconsin.

13. Weng Lin Tang, Ph.D., August 2010, “Engineering of an Efficient and Enantioselective

Biocatalyst for the Preparation of Chiral Pharmaceutical Intermediates.” Now research

scientist at Codexis @ Singapore, Singapore.

14. Matt DeSieno, Ph.D., August 2011, “Microbial Synthesis of Antimalarial Compound FR-

900098: Pathway Characterization and Engineering.” Now research scientist at Pfizer,

NJ.

15. Jing Du, Ph.D., August 2011, “Metabolic Engineering of Saccharomyces Cerevisiae for

Efficient Ethanol Production from Pentose Sugars.” Now research scientist at

Novozymes, Franklinton, NC.

16. Ning Sun, Ph.D. June 2013, “Engineering of Transcriptional Activator-like Effector

Nucleases (TALENs) for Targeted Genome Editing.” Now research scientist at Abbvie,

Chicago, IL.

17. Jing Liang, Ph.D. March 2014, “Cellular Reprogramming in Eukaryotes.” Now research

scientist at Metabolic Engineering Research Laboratory, Agency for Science, Technology

and Research, Singapore.

70

18. Carl Denard, Ph.D. April 2014, “Engineering Novel Tandem Reactions Using

Organometallic Catalysts and (Metallo)Enzymes.” Now assistant professor at the

University of Florida, Gainesville

19. Yunzi Luo, Ph.D. April 2014, “Natural Products Discovery and Characterization via

Synthetic Biology.” Now professor at Sichuan University, China.

20. Dawn Eriksen, Ph.D. May 2014, “Combinatorial Biosynthetic Pathway Engineering for

Microbial Production of Biofuels.” Now research scientist at Pfizer, MA.

21. Sijin Li, Ph.D. June 2014, “Strain Engineering and Biosensor Development for Efficient

Biofuel Production by Saccharomyces cerevisiae.” Now assistant professor at the

Department of Chemical and Biomolecular Engineering at Cornell University.

22. Tong Si, Ph.D. August 2014, “Genome Scale Engineering in Saccharomyces cerevisiae.”

Now professor at the Chinese Academy of Sciences Shenzhen Institute of Advanced

Studies, China.

23. Ryan Cobb, Ph.D. December 2014, “Natural Product Discovery and Engineering at the

Protein, Pathway and Genome Scales.” Now research scientist at Dow AgroSciences, IN.

24. Zhanar Abil, Ph.D. April 2015, “Study and Engineering of Nucleic Acid-Binding Repeats

Proteins.” Now postdoc at University of Texas at Austin.

25. Jiazhang Lian, Ph.D. May 2015, “Engineering Saccharomyces cerevisiae with Enhanced

Supply of Precursor Metabolites for Efficient Production of Fuels and Chemicals.” Now

professor at Zhejiang University, China.

26. Todd Freestone, Ph.D. June 2016, “Pathway Engineering for the Discovery and

Optimized Production of Phosphonic Acids.” Now research scientist at Pfizer, MI.

27. Zehua Bao, Ph.D. May 2017, “Multiplex Genome Editing and Gene Regulation in

Eukaryotic Cells.” Now postdoc at Boston University, MA.

28. Mohammad Hamedi Rad, Ph.D. November 2017, “Development and Application of

Computational Tools for Metabolic Engineering.” Now Chief Operation Officer at

LifeFoundry, Champaign, IL.

29. Yajie Wang, Ph.D. December 2018, “Design and Engineering of Multi-step

(Bio)Catalytic Systems.” Now postdoc at UIUC

30. Hengqian Ren, Ph.D. April 2018, “Development and Application of Synthetic Biology

Tools for Natural Product Research.”

31. Surbhi Jain, Ph.D. April 2020, “Mammalian Genome Engineering: Fundamental

Investigation and Method Development.”

32. Ipek Tasan, Ph.D. April 2020, “New Tools for Live Cell Imaging of Endogenous Loci in

Mammalian Cells.”

Visiting Ph.D. Thesis Students

1. Jie Sun, Ph.D. East China University of Science and Technology, June 2012,

“Consolidated Bioprocessing of Hemicellulose.” Now Visual Arts Specialist, North

Carolina State University

2. Sai Wen, Ph.D. Beijing University of Chemical Technology, December 2012, Now

assistant professor in Beijing

3. Jinglin Li, Ph.D. Konkuk University

4. Lu Zhang, Ph.D. Tianjin University

5. Sujit Jagtag, Ph.D., Konkuk University

71

6. Yijun Guo, visiting graduate student from University of Science and Technology of

China

Current: 19

1. Ran Cao, Ph.D., anticipated 2016

2. Xiong Xiong, Ph.D. anticipated 2017

3. Ipek Tasan, Ph.D., anticipated 2018

4. Behnam Enghiad, Ph.D., anticipated 2018

5. Surbhi Jain, Ph.D. anticipated 2019

6. Shekhar Mishra, Ph.D. anticipated 2020

7. Meng Zhang, Ph.D. anticipated 2021

8. Pu Xue, Ph.D. anticipated 2021

9. Qiqi Tian, Ph.D. anticipated 2021

10. Che Yang, Ph.D. anticipated 2022

11. Lam Voh, Ph.D. anticipated 2022

12. Vinh Tran, Ph.D. anticipated 2022

13. Carl Schultz, Ph.D. anticipated 2020

14. Bryan Mejia-Sosa, Ph.D. anticipated 2023

15. Chengyou Shi, Ph.D. anticipated 2023

16. Michael Volk, Ph.D. anticipated 2023

17. Tianhao Yu, Ph.D. anticipated 2024

18. Daniel Xun, Ph.D. anticipated 2024

19. Aashutosh Boob, anticipated 2024

B. Supervision of Postdocs/Research Associates

Former: 31

1. Tongbo Zhu, 2002-2003, unknown

2. Olga Esteban, 2002-2004, unknown

3. Jung-kul Lee, 2004-2006, now professor of chemical engineering at the Konkuk

University, Seoul, South Korea

4. Ryan Woodyer, 2005-2006, now research scientist at Tate & Lyle, Decatur, IL

5. Hua Zhao, 2006-2007, now a research scientist at the Institute of Chemical Engineering

and Science (ICES), Singapore

6. Yoo Seong Choi, 2006-2008, now assistant professor of chemical engineering at the

Chungnam University, South Korea

7. Haige Lu, 10/2007-10/2008, now a postdoc researcher at the Memorial Sloan-Kettering

Cancer Center, New York

8. Yuichi Nakagawa, 1/2008-10/2008, unknown

9. Michael Vu, 5/2008-5/2009, now a postdoc researcher at Rice University, Houston, TX

10. Tae Hee Lee, 6/2008-6/2010, now research scientist at the CJ Bio R&D Center, South

Korea

11. Won-seok Jung, 3/2009-7/2010, now research scientist at the Samsung Biotech, South

Korea

12. Amit Ghosh, 1/2009-5/2011 (joint supervised with Nathan Price), now research scientist

at the Joint Bioenergy Institute, Emerville, CA

72

13. Byoungjin Kim, 1/2009-12/2011, now postdoc at KAIST, Korea.

14. Levi Stanley, 1/2011-12/2011 (jointly supervised with John Hartwig), now assistant

professor of chemistry at the Iowa State University

15. Yunfeng Hu, 1/2011-1/2012, now postdoc at Polytechnic University of New York

16. Yongbo Yuan, 11/2010-3/2012, now postdoc at the University of Delaware

17. Zengyi Shao, 4/2009-12/2012, now assistant professor of chemistry at the Iowa State

University

18. Dan Coursolle, 11/2011-4/2013, now research scientist at Cargill, Iowa

19. Refaat Hamed, 6/2013-3/2014, now Assistant Professor, Assiut University, Egypt

20. Xueyang Feng, 3/2012-5/2014, now assistant professor at Virginia Tech, Blacksburg, VA

21. Hua Huang, 5/2012-8/2014, now postdoc with John Gerlt, UIUC

22. Han Xiao, 8/2012-2/2015, now associate professor at Shanghai Jiaotong University,

China

23. Yunzi Luo, 5/2014-5/2015, now professor at Sichuan University, China

24. Meng Wang, 1/2010-6/2015, now professor at Chinese Academy of Sciences Tianjin

Institute of Industrial Biotechnology, China

25. Sijin Li, 8/2014-7/2015, assistant professor at the Department of Chemical and

Biomolecular Engineering at Cornell University

26. Shangwen Luo, 6/2015-6/2016, unknown

27. Eva Garcia Ruiz, 7/2013-6/2016, now postdoc at the University of Edinburg

28. Jiazhang Lian, 8/2015-11/2017, now professor at Zhejiang University

29. William Streyer, 4/2017-8/2018, unknown

30. Yi Yu, 5/2016-5/2018, now scientist at Beam Therapeutics

31. Tong Si, 8/2014-4/2019, now professor at the Chinese Academy of Sciences Shenzhen

Institute of Advanced Studies, China

Current: 11

1. Bin Wang, 3/2016-date

2. Fang Guo, 6/2016-date

3. Teresa Martin, 10/2016-date

4. Zia Fatma, 7/2018-date

5. Mingfeng Cao, 6/2018-date

6. Chunshuai Huang, 10/2018-date

7. Yajie Wang, 1/2019-date

8. Stephen Lane, 2/2019-date

9. Xiaoqiang Huang, 3/2019-date

10. Hengqian Ren, Ph.D., 5/2019-date

11. Guangde Jiang, Ph.D. 9/2019-date

C. Supervision of Visiting Professors

Former: 7

1. Whankoo Kang, Professor of Chemical Engineering, Hannam University, Korea, 2001.

2. Chun Li, Professor of Chemical Engineering, Beijing Institute of Technology, 2011-

2012.

73

3. Yu Jiang, Associate Professor of Center for Industrial Biotechnology, Chinese Academy

of Sciences, Shanghai, China, 2011-2012.

4. Qianfu Wang, Associate Professor, Shanghai Institute of Plant Physiology and Ecology,

Chinese Academy of Sciences, 5/2012-11/2012.

5. Zhen Kang, Associate Professor, Jiangnan University, China, 3/2015-3/2016

6. Ayca Zeynep Ilter, Yeditepe University, Istanbul, Turkey, 9/2017-7/2018

7. Xiao-wei Yu, Associate Professor, Jiangnan University, China, 9/2018-8/2019

Current: 0

D. Supervision of Undergraduate Students

Former: 62

Current: 6

1. Trang Vu, 2005-2007

2. Frank Qin, 2005-2006

3. Mark Laurenz, 2005-2006

4. Ka-chun Lai, 2004-2006

5. Chae Young Han, 2005

6. Junaid Begawala, 2004

7. Kathleen Sese, 2004

8. Kyle Kloepper, 2004

9. Samir Shah, 2001

10. Anna Kornafel, 2001-2002

11. Peter Meis, 2001-2002

12. Edgar Goluch, 2001-2003

13. Yen Sia Low, 2002-2003

14. Andrew Miller, 2002-2003

15. Thuytram Dang, 2004

16: Saroj Baha, 2006-2007

17. Anu Biswas, 2006-2008

18. Jenna Wozniak, 2006-2008

19. Kyle Fritschle, 2007-2008

20. Vincent Ip, 2008-2010

21. Erik Anderson, 2009

22. Tanush Sahay, 2009-2010

23. Helen Pei Chiun Hsieh, 2010-2011

24. Daniel Lee, 2010

25. Danish Haider, 2010-2012

26. Braden Christian, 2010-2011

27. Lu Lu, 2011-2012

28. Patrick Lynn, 2011-2012

29. Amy Oreskovic, 2012-2013

30. Wei Yang, 2012-2013

31. Zachary Dookeran, 2012-2013

32. Lily Chen, 2012-2013

74

33. Sherrie Lim Xuan Lu, 2013

34. Stella Wu, 2013-date

35. Justin Chacko, 2013

36. Run Jin, 2013-2014

37. Aaron Chen, 2013-2014

38. Kate Barton, 2013-2014

39. Hejun Li, 2013-2014

40. Robert Pipal, 2014

41. Chenzhao Yu, 2014-2015

42. Xinyue Qi, 2014-2015

43. Valerie Jaroenpuntaruk, 2014-2016

44. Yuhao Min, 2015-2018

45. Yuying Wu, 2015-2017

46. Wen Ren, 2015-2016

47. Ke Zhang, 2015-2017

48. Yiran Xu, 2015-2017

49. Justin Genova, 2015-2017

50. Linyang Ju, 2015-2017

51. Sherri Ho, 2015-2018

52. Sumeng Hu, 2016-2018

53. Yiziying Chen, 2016-2018

54. Scott Weisberg, 2016-2019

55. Pingfan Hu, 2016-2017

56. Christina Su, 2017-date

57. Hugh Yeh, 2017-2019

58. Lingzixuan Zhang, 2017-2020

59. Yuanqing Lan, 2017-2019

60. Harpal Singh, 2018

61. William Lyon, 2018-2019

62. Emlia Pieta, 2018-date

63. Ziyu Wang, 2018-date

64. Shireen Abesteh, 2018-2019

65. Canal Li, 2018-2019

66. Emilia Pieta, 2018-date

66. Yimeng Zhao, 2019-date

67. Andrea Mejia, 2019-date

68. Srija Matukumalli, 2019-date

E. Supervision of Rotation Students and Biophysics Tutorial Students

Rotation Students: 63

1. Zhilei Chen (Biophysics), 2001

2. Haili Pin (Biophysics), 2001

3. Fenglin Yin (Biophysics), 2002

4. Marina Barakova (Biophyscis), 2002

5. Shu Dong (CSB), 2002

75

6. Sheryl Rubin-Pitel (ChBE), 2003

7. Michael McLachlan (Biophysics), 2004

8. Hua Zhou (Biochemistry), 2005

9. Meng Chen (CSB), 2005

10. Nell Keith (BioE), 2005

11. Kara Smith (ChBE), 2005

12. Alex Parent (Chem), 2005

13. Victor Gonzalez (Chem), 2005

14. Quin Christensen (Micro), 2005

15. Brad Evans (Biochemistry), 2005

16. Po-chao Wen (Biophysics), 2005

17. Yanni Lin (MCB), 2007

18. Hui Liu (Biophysics), 2007

19. Xiaoyi Cao (Biophysics), 2007

20. Ning Sun (Biochemistry), 2007

21. Cancan Huang (Biophysics), 2007

22. Jesse Grenz (MCB), 2008

23. Abhinav Luthra (MCB), 2008

24. Neha Garg (MCB), 2008

25. Salehe Ghasempur (MCB), 2008

26. Ryan Cobb (ChBE), 2008

27. Zhanar Abil (MCB), 2009

28. Emilia Calvaresi (MCB), 2009

29. Tao Jiang (Biophysics), 2009

30. Kyle Dunbar (Chemistry), 2009

31. Chang Sun (Biochemistry), 2010

32. Takanori Miyairi (Biophysics), 2010

33. Yue Hao (Biochemistry), 2010

34. Manuel Ortega (Biochemistry), 2010

35. Seyfullah Kotil (Biophysics), 2010

36. Kevin Whitley (Biophysics), 2010

37. Xinyu Cao (Biochemistry), 2011

38. Zehua Bao (Biochemistry), 2011

39. Zhe Shen (Biochemistry), 2011

40. Mahdyie Jadaliha (Biochemistry), 2011

41. Maryam Kh (Biochemistry), 2012

42. Ipek Tasan (Biochemistry), 2012

43. Xiaobin Zheng (Biochemistry), 2012

44. Tas Hussein (Biophysics), 2012

45. Bhoomika Mathur (Biochemistry), 2013

46. Yuanyuan Hu (Microbiology), 2013

47. Erik Andersen (MCB), 2014

48. Soumitra Athavale (MCB), 2014

49. Surbhi Jain (MCB), 2014

50. ShengShee "Seth" Thor (Biophysics), 2014

76

51. Zach Schaefer (MCB), 2015

52. Gulishana "Lisa" Adilijiang (MCB), 2015

53. Marc Gancayco (Chemistry), 2015

54. Mustafa Dane (MCB), 2015

55. Adam Dicaprio (Chemistry), 2015

56. Xuejiao Song (Biochemistry), 2016

57. Qiqi Tian (Biochemistry), 2016

58. Nic Handy (MCB), 2017

59. Kasra Tabatabaei (biophysics), 2017

60. Che Yang (MCB), 2017

61. Andrew Blake (Biophysics), 2017

62. Ruicheng Shi (MCB), 2018

63. Lindsey Bendix (MCB), 2018

Tutorial Students (7):

1. Rong Cao, 2005

2. Qian Bian, 2005

3. Ayano Sakai, 2007

4. Xiaowen Hou, 2010

5. Yang Yu, 2013

6. Alex Moffett, 2015

7. Jordan Sickle, 2018

F. Supervision of Visiting Students

1. Kazi Islam, Georg-August-University Goettingen (Germany), 2006

2. Jie Sun, East China University of Science and Technology (China), 2008-2010

3. Guiomar Sanchez Carron, University of Murcia (Spain), 2010

4. Jinglin Li, Konkuk University, 2010-2011

5. Sai Wen, Beijing University of Chemical Technology (China), 2010-2011

6. Mirko Beissner, Technical University of Munich, Spring, 2012

7. Sujit Jagtag, Konkuk University, 2012-2014

8. Lu Zhang, Tianjin University, 2012-2014

9. Yijun Guo, University of Science and Technology of China, 2017-2018

10. Xiaofei Song, Nankai University, 2018-2019

G. Service on Ph.D. Examination Committees

Preliminary Examination Committees (108)

2001, Zheng-guang Wang, Chemical Engineering

2002, Josh Ackerman, Chemical Engineering

2002, M. Larid Forrest, Chemical Engineering

2002, Qingjun Wang, Biophysics

2003, John Comninos, Chemistry

2003, Eric Olsen, Chemistry

2003, Su Ha, Chemical Engineering

2003, Halong Vu, Chemical Engineering

77

2003, Ryan Woodyer, Chemistry, Chair

2004, Karu Chockalingam, Chemical Engineering, Chair

2004, Zhilei Chen, Biophysics, Chair

2004, Emily J. Pollauf, Chemical Engineering

2004, Dan Ryan, Chemistry

2004, Chandrashekar Raman, Chemical Engineering

2005, Ee-Lui Ang, Chemical Engineering, Chair

2005, Michael Toepke, Chemical Engineering

2005, Edgar Goluch, Bioengineering

2005, Matt Levengood, Chemistry

2005, YoungJung Chang, Chemical Engineering

2005, Josh Ramsey, Chemical Engineering

2006, Wei Xie, Chemical Engineering

2006, Wenjuan Zha, Chemical Engineering, Chair

2006, Tyler Johannes, Chemical Engineering, Chair

2006, Paul Barone, Chemical Engineering

2006, Fenglin Yin, Biophysics

2007, Keng Jin Lee, Chemical Engineering

2007, Nathan Gabrielson, Chemical Engineering

2007, Zengyi Shao, Chemical Engineering, Chair

2007, David Drake, Chemical Engineering

2007, Quanming Shi, Chemical Engineering

2007, Tasha Desai, Chemical Engineering

2008, Sheryl Rubin-Pitel, Chemical Engineering

2008, Fei Wen, Chemical Engineering

2008, Ryan Sullivan, Chemical Engineering

2008, Lily Wong, Chemical Engineering

2008, Quinn Peterson, Biochemistry

2008, Yan Fan, Chemistry

2008, Sharon Hyonju Choi, Biochemistry

2008, Jung-un Baek, Chemistry

2009, Kang Wu, Chemical Engineering

2009, Divina Anunciado, Chemistry

2009, Benjamin Schudel, Chemical Engineering

2009, Matt DeSieno, Chemical Engineering


2010, Khushnuma Koita, Chemical Engineering

2010, Chieh Chun Chen, Bioengineering

2010, Daniel Ong, Chemical Engineering

2010, Vinayak Agarwal, Biophysics

2010, Devine Whipple, Chemical Engineering

2010, Noel Xu, Chemical Engineering

2010, John Schmidt, Chemical Engineering

2011, Rahul K. Keswani, Chemical Engineering

2011, Neha Garg, Biochemistry

78

2011, Carolyn Milne, Chemical Engineering

2011, Francisco Guerra, Biophysics

2011, Ismaeel Muhamed, Biochemistry

2011, Mark Hwang, Medical Scholar Program

2011, Guodong Rao, Chemistry

2011, Weixin Tang, Chemistry

2011, Soo Rin Kim, Food Sciences

2012, Kyong Wook Noh, Chemical Engineering

2012, Timothy Turner, Food Sciences

2012, Yuliang Wang, Chemical Engineering

2012, Mathew Benedict, Chemical Engineering

2012, Yujie Xia, Chemical Engineering

2012, Subha Mukherjee, Chemistry

2012, Ryan Cobb, Chemical Engineering

2012, Yunzi Luo, Chemical Engineering

2012, Sijin Li, Chemical Engineering

2012, Caitlin Deane, Chemistry

2013, Mei-Hsiu Lai, Chemical Engineering

2013, Shuyi Ma, Chemical Engineering

2013, Binhui Zhao, Molecular and Cell Biology

2013, Yue Hao, Biochemistry

2013, Shannon Walsh, Biochemistry

2013, David Parker, Biochemistry

2013, Mathew Richards, Chemical Engineering

2013, Jiazhang Lian, Chemical Engineering

2014, Nektaria Petronikolou, Biochemistry

2014, Suyan Zhang, Chemical Engineering

2014, Ahmet Badur, Chemical Engineering

2015, Jing Jiang, Electrical and Computer Engineering

2015, Ran Chao, Chemical Engineering

2015, Jonathan Ning, Chemical Engineering

2015, Sam Hamed, Chemical Engineering

2016, Jiewen Zhou, Chemical Engineering

2016, Yajie Wang, Chemical Engineering

2016, Hengqian Ren, Chemical Engineering

2017, Kisurb Choe, Microbiology

2017, Behnam Enghiad, Chemical Engineering

2017, Antony Sotelo, Chemistry

2017, Alex Moffett, Biophysics

2017, Saurabh Shukla, Chemical Engineering

2017, Lawrence Wang, Biochemistry

2017, Joseph Ellis, Chemistry

2018, Chayanid Ongpipattanakul, Biochemistry

2018, Thomas Pawel, Chemistry

2018, Shekhar Miresh, Chemical Engineering

79

2019, Carl Schultz, Chemical Engineering

2019, Santanu Ghosh, Biochemistry

2019, Dustin Buntrock, Biochemistry

2019, Adrika Raybarman, Biochemistry

2019, Mason Xue, Chemical Engineering

2019, Meng Zhang, Chemical Engineering

2019, Tahere Mokhtari, Chemical Engineering

2020, Vinh Tran, Chemical Engineering

2020, Matthew Chan, Chemical Engineering

2020. Mingkuan Lyu, Chemistry

Final Thesis Examination Committees (97):

2002, Qingjun Wang, Biophysics

2003, Zheng-guang Wang, Chemical Engineering

2003, Josh Ackerman, Chemical Engineering

2003, M. Larid Forrest, Chemical Engineering

2003, Yu-wen Huang, Chemical Engineering

2004, Emily J. Pollauf, Chemical Engineering

2005, Anil Prakasam, Chemical Engineering

2005, Chandrashekar Raman, Chemical Engineering

2005, Ryan Woodyer, Chemistry, Chair

2005, Bridget Trogden, Chemistry

2006, Karu Chockalingam, Chemical Engineering, Chair

2006, Zhilei Chen, Biophysics, Chair

2006, Michael Toepke, Chemical Engineering

2006, Heather Relyea, Chemistry

2006, Josh Ramsey, Chemical Engineering

2006, YoungJung Chang, Chemical Engineering


2007, Wei Xie, Chemical Engineering

2007, Ee Lui Ang, Chemical Engineering, Chair

2007, Wenjuan Zha, Chemical Engineering, Chair


2008, Tyler Johannes, Chemical Engineering, Chair

2008, Fenglin Yin, Biophysics

2008, Monica Usrey, Chemical Engineering

2008, Keng Jin Lee, Chemical Engineering

2008, Paul Barone, Chemical Engineering

2008, Matt Levengood, Chemistry

2009, Nathan Gabrielson, Chemical Engineering

2009, Zengyi Shao, Chemical Engineering, Chair

2009, David Drake, Chemical Engineering

2009, Daniel Ryan, Chemistry

2009, Lily Wong, Chemical Engineering

2009, Tasha Desai, Chemical Engineering

80

2009, Yan Fan, Chemistry

2009, Quanming Shi, Chemical Engineering

2009, Sheryl Rubin-Pitel, Chemical Engineering, Chair

2009, Ryan Sullivan, Chemical Engineering, Chair

2010, Benjamin Schudel, Chemical Engineering

2010, Fei Wen, Chemical Engineering, Chair

2010, Nikhil Nair, Chemical Engineering, Chair

2010, Divina Anunciado, Chemistry

2010, Michael McLachlan, Biophysics, Chair

2011, Kevin Lin, Chemical Engineering

2011, Robert Morgan, Chemical Engineering

2011, Devin Whipple, Chemical Engineering

2011, Matthew DeSieno, Chemical Engineering

2011, Jing Du, Chemical Engineering

2011, John Comminos, Chemistry

2012, Rahul Keswani, Chemical Engineering

2012, Mark Hwang, MD/PhD Program


2012, Soo-Rin Kim, Food Sciences

2012, Vinayak Agarwal, Biochemistry

2012, Chieh-Chun Chen, Bioengineering

2013, Sze Wei Daniel Ong, Chemical Engineering

2013, Caroline Milner, Chemical Engineering

2013, Ning Sun, Biochemistry

2013, Neha Garg, Biochemistry

2013, Kyong Wook Noh, Chemical Engineering

2014, Matthew Benedict, Chemical Engineering

2014, Arnab Mukherjee, Chemical Engineering

2014, Carl Denard, Chemical Engineering

2014, Yunzi Luo, Chemical Engineering

2014, Jing Liang, Chemical Engineering

2014, Dawn Eriksen, Chemical Engineering

2014, Sijin Li, Chemical Engineering

2014, Tong Si, Chemical Engineering

2014, Mei-Hsiu Lai, Chemical Engineering

2014, Josh Quarterman, Food Science and Human Nutrition

2014, Ryan Cobb, Chemical Engineering

2015, Pei Wang, Biochemistry

2015, Weixin Tang, Chemistry

2015, Zhanar Abil, Biochemistry

2015, Jiazhang Lian, Chemical Engineering

2015, Rich Harrison, Chemical Engineering

2015, Subha Mukherjee, Chemistry

2015, Parisa Hosseinzadeh, Biochemistry

2015, Yue Hao, Biochemistry

81

2016, Matthew Richards, Chemical Engineering

2016, Shuyan Zhang, Chemical Engineering

2016, Todd Freestone, Chemical Engineering

2017, Jing Jiang, Electrical and Computer Engineering

2017, Zehua Bao, Biochemistry

2017, Sam Hamedi, Chemical Engineering

2017, Nektaria Petronikolou, Biochemistry

2018, Jiah Kim, Molecular and Cellular Biology

2018, Yajie Wang, Chemical Engineering

2019, Abraham Wang, Chemistry

2019, Hengqian Ren, Chemical Engineering

2019, Binhui Zhao, Molecular and Cellular Biology

2019, Alex Moffett, Biophysics

2020, Liguo Zhang, Molecular and Cellular Biology

2020, Surbhi Jain, Biochemistry

2020, Ipek Tasan, Biochemistry

2020, Saurabh Shukla, Chemical Engineering

2020, Joanna Ellis, Chemistry

2020, Chuankai Zhao, Chemical Engineering

IV. SERVICE (PUBLIC, PROFESSIONAL/DISCIPLINARY, AND UNIVERSITY)

A. Public Service

Consulting Service

Member of Scientific Advisory Board, AgriMetis, DC, 2016-date

Member of Scientific Advisory Board, Toulouse White Biotechnology, Toulouse, France,

2016-date

Member of Scientific Advisory Board, Gevo, Denver, CO, 2006-2010

Member of Scientific Advisory Board, Myriant, Woburn, MA, 2009-2013

OMT, Inc., Palo Alto, CA, 2008-2009

zuChem, Peoria, IL, 2007-2010

Maxygen, Redwood City, CA, 2005-2010

British Petroleum (BP), Naperville, IL, 2007-2008

Pfizer, Groton, CT, 2008

Cabot Corporation, Boston, MA, 2002

Fox/Atkins Development, Urbana, IL, 2002

The Dow Chemical Company, Midland, MI, 1997

Diversa, Inc., San Diego, CA, 1996

Service to Government Agencies

Advisors:

DOE Biological and Environmental Research Program, 2012-date

Review Panels:

NSF SBIR/STTR Phase I, Bio-based Sensors, Washington, DC, March 24-25, 2003

82

NIH Biochemistry Study Section, Washington, DC, November 13-14, 2003

NSF SBIR/STTR Phase I, Bio-based Sensors, Washington, DC, April 5-6, 2004

NSF Metabolic Engineering, Washington, DC, April 27-28, 2005

NSF SBIR/STTR Phase I, Industrial Bioproducts, Washington, DC, March 14-15, 2006.

NSF BES Biochemical Engineering/Biotechnology, Washington, DC, April 20-21, 2006.

NSF BES CAREER review panel, Washington, DC, November 8-9, 2006.

NSF CBET Biochemical Engineering/Biotechnology, Washington, DC, December 3-4, 2007.

NSF BES Biochemical Engineering/Biotechnology Review Panel, Washington, DC,

December 10-11, 2008.

DOE Site Review Panel for Joint BioEnergy Institute (JBEI), Emeryville, CA, September 30

to October 1, 2009

DOE Year Three Review Panel of DOE’s Three Bioenergy Research Centers, September 26-

30, 2010

NIH Interdisciplinary Molecular Sciences and Training Integrated Review Group (IRG),

Washington, DC, March 10-11, 2011

NSF Review Panel on Synthetic Biology & Metabolic Engineering, Washington, DC, Jan.

20-21, 2015

Reviewer for Grant Proposals:

National Institutes of Health (NIH)

National Science Foundation (NSF)

Army Research Office (ARO)

ARO Institute for Collaborative Biotechnologies

Department of Energy (DOE)

US Department of Agriculture (USDA)

ACS Petroleum Research Fund

Research Corporation

North Dakota State Government

Chinese National Science Foundation

Stanford University

Workshops:

ARO’s Workshop for Bio-Fuel Cells, Washington, DC, June 30-July 2, 2002

DOE’s Workshop on Catalysis, Gaithersburg, Maryland, May 14-16, 2002

U.S. Government’s Science and Technical Expert Partnership (STEP) Program Workshop on

Synthetic Biology, McLean, VA, July 27, 2005.

ARO’s Workshop on Cell-Like Entities (CLE), Fairborn, OH, September 13-14, 2005.

Chinese Academy of Sciences-Max Planck- Gesellschaft Exploratory Round Table

Conferences on Synthetic Biology, Shanghai, October 19-21, 2010.

DOE’s Workshop on Genome Engineering for Materials Synthesis, October 10-11, 2018

Search Committees:

Argonne National Laboratories Biosciences Division Director Search Committee, 2007-2008

B. Service to Disciplinary and Professional Societies and Associations

National Academy of Engineering (NAE)

Co-organizer, NAE’s Indo-American Frontiers of Engineering Symposium, March, 2012

American Institute of Chemical Engineers (AIChE)

83

Co-chair, Advances in Biocatalysis and Protein Engineering, AIChE Annual Meeting, Reno,

CA, November 5, 2001.

Chair, Combinatorial/Directed Evolution Approaches in Bioengineering, AIChE Annual

Meeting, San Francisco, CA, November 20, 2003.

Chair, Advances in Metabolic Engineering, AIChE Annual Meeting, Austin, TX, November

5-12, 2004.

Chair, Advances in Metabolic Engineering, AIChE Annual Meeting, Cincinnati, OH,

October 30 - November 4, 2005.

American Chemical Society

Co-chair, High throughput Screening/Genomics and Proteomics, ACS Annual Spring

Meeting, Anaheim, CA, March 28-April 1, 2004.

Co-chair, Protein Expression, ACS Annual Spring Meeting, San Diego, CA, March 13-17,

2005.

Co-chair, Protein Expression, ACS Annual Fall Meeting, San Francisco, CA, September 10-

14, 2006.

Symposium Chair, Emerging Technologies, ACS Annual Fall Meeting, Boston, MA, August

19-23, 2007.

Co-chair, Advances in Biocatalysis, ACS Annual Fall Meeting, Philadelphia, CA, August 17-

21, 2008.

Programming Chair, BIOT Division, ACS Annual Fall Meeting, Washington DC, August 16-

20, 2009.

Institute of Electrical and Electronic Engineers (IEEE)

Theme chair, Molecular, Cellular and Tissue Engineering and Biomechanics, 26th Annual

International Conferences of the IEEE Engineering in Medicine and Biology Society, San

Francisco, CA, September 1-4, 2004.

Member of Program Committee, 4th IEEE International Symposium on Bioinformatics and

Bio Engineering, Taichung, Taiwan, May 19-21, 2004.

Society for Industrial Microbiology and Biotechnology (SIMB)

Session chair, Recent Advances in Biocatalyst Development, Annual SIM Meeting, Denver,

CO, July 29 - August 2, 2007.

Program co-chair, Biocatalysis, Annual SIM Meeting, Denver, CO, July 29-August 2, 2007.

Program co-chair, Biocatalysis, Annual SIM Meeting, San Diego, CA, August 10-14, 2008.

Program Chair, Biocatalysis, Annual SIM Meeting, Toronto, Canada, July 26-30, 2009.

Program co-Chair, Natural Products, Annual SIM Meeting, New Orleans, LA, July 24-28,

2011

Program Chair, Natural Products, Annual SIM Meeting, Washington DC, August 12-16,

2012

Program Chair, Natural Products, Annual SIM Meeting, St. Louis, MO, July 20-24, 2014

Session Chair, Overproduction of Natural Products, Annual SIM Meeting, Philadelphia, PA,

August 3-7, 2015

Engineering Conference International (ECI)

Session chair, Evolutionary Approaches in Metabolic Engineering, Metabolic Engineering V

International Conference, Lake Tahoe, CA, September 19-23, 2004.

84

Session chair, Biomolecular Evolutions and Revolutions: DNA, RNA, and Proteins,

Biochemical Engineering XV International Conference, Quebec City, Canada, July 15-19,

2007

Program Co-chair, Biochemical and Molecular Engineering XVIII International Conference,

Beijing, June 16-21, 2013

Advisory Committee Member, ECI's Biochemical and Molecular Engineering XVIV,

Mexico, 2014-2015

Advisory Committee Member, ECI's Enzyme Engineering XXIV, Toulouse, France, 2016-

2017

Program Chair, ECI's Enzyme Engineering XXV, 2019.

Reviewer for:

Science

Nature

Nature Biotechnology

Nature Methods

Nature Chemical Biology

Nature Reviews Microbiology

Nature Chemistry

Nature Catalysis

Nature Communications

Nature Protocols

Journal of the American Chemical Society (JACS)

Angewandte Chemie

PNAS

Nucleic Acids Research

Journal of Biological Chemistry

Journal of Molecular Biology

ACS Chemical Biology

ACS Synthetic Biology

Chemistry and Biology

Molecular Microbiology

Biochemistry

Analytical Chemistry

Applied and Environmental Microbiology

Journal of Immunological Methods

Biotechnology and Bioengineering

Biotechnology Progress

Biotechnology Journal

Biotechniques

Bioinformatics Journal

Biochimica et Biophysica Acta (BBA)

FEBS Letters

FEMS Microbiology Letters

Metabolic Engineering

85

Trends in Biotechnology

Trends in Microbiology

Extremophiles

Molecular Biology and Evolution

Microbial Cell Factories

Biotechnology for Biofuels

Current Opinion in Biotechnology

Current Opinion in Chemical Biology

Chemical Communications

Cell Research

Cellular and Molecular Life Sciences

Tetrahedron

Expert Opinion in Biological Therapy

Journal of Molecular Catalysis B: Enzymatic

Journal of Bioscience and Bioengineering

Systems and Synthetic Biology

Organic Letters

C. University/Campus Service

UIUC Campus

Office of Technology Management (OTM) Board of Advisors 2003-2013

Roy J. Carver Biotechnology Center Advisory Committee 2009-2014

Chancellor/Provost Faculty Consultation Group 2014-2016

Campus Selection Panel for the Global Young Scientists Summit in Singapore 2015-2016

College of Engineering

Ad Hoc Subcommittee to Review a Proposal to Revise Bioengineering Minor 2006

Executive Committee 2007-2010

College of Medicine

Research Advisory Board 2017-date

College of Liberal Arts and Science

100th Anniversary Planning Committee 2012-2013

LAS Nominations Committee 2015-2017

School of Chemical Sciences

ChBE Department Head Search Committee 2002-2003

Executive Committee 2007-2010

Faculty Advisor for Chemical Storeroom 2008-date

Search Committee for SCS Associate Director 2008

Search Committee for SCS Business and Finance Specialist 2009

Search Committee for SCS Storeroom Coordinator 2009

Search Committee for SCS Director 2012

Department of Chemical and Biomolecular Engineering

Academic Advisor for Undergraduate Students in Chemical Engineering 2000-date

Administrative Committee 2000-date

Graduate Admission Committee 2000-2007

Systems Bioinformatics Committee 2001-2003

86

Graduate Fellowship Coordinator 2003-2007

Undergraduate Curriculum Committee 2004-2006

Shen Postdoctoral Fellowship 2005

Chair, Departmental Seminar Committee 2004-2007

Faculty Search Committee 2005-2007

Chair, Biomolecular Engineering Minor Committee 2006

Chair, Undergraduate Curriculum Committee 2010

Chair, Awards Committee 2010-2013

Awards Committee 2013-2015

Faculty Search Committee 2010-date

Faculty Search Committee on Computational Systems Biology 2013-2014

Department of Biochemistry


Department of Bioengineering

Co-chair, Departmental Seminar Committee 2003-2004

Executive Committee 2015-date

Department of Food Science and Human Nutrition

Faculty Search Committee for Position in Microbial Genomics 2005-2007

Department of Microbiology

Faculty Search Committee for Position in Bioinformatics 2005-2006

Faculty Search Committee for Position in Natural Products 2006-2007

Center for Biophysics and Computational Biology



School of Molecular and Cell Biology

NIH Cellular and Molecular Biology Training Grant Minority Committee 2003

Molecular Biophysics Training Grant Executive Committee 2008-2011 &

2016-date

D. Service to Other Universities/National Laboratories

Argonne National Laboratories

Search Committee for Director of the Division of Biosciences 2007-2008

87

Zhao group members in Academia

Graduate Students

1. Zhilei Chen (2000-2006): Associate Professor, Department of Chemical Engineering,

Texas A&M University, Station College, TX

2. Tyler Johannes (2002-2008): Associate Professor, Department of Chemical Engineering,

University of Tulsa, Tulsa, OK

3. Ee Lui Ang (2002-2004): PI, Institute of Chemical Engineering and Science, Singapore

4. Fei Wen (2003-2010): Assistant Professor, Department of Chemical Engineering,

University of Michigan, Ann Arbor, MI

5. Nikhil Nair (2004-2010): Assistant Professor, Department of Chemical Engineering,

Tufts University, Boston, MA

6. Zengyi Shao (2002-2008): Assistant Professor, Department of Chemical Engineering,

Iowa State University, Ames, IA

7. Yunzi Luo (2008-2014): Professor, Sichuan University, China

8. Jiazhang Lian (2010-2015): Professor, Zhejiang University, China

9. Sijin Li (2008-2014): Assistant Professor, Cornell University

10. Tong Si (2009-2014): Professor, CAS Shenzhen Institute of Advanced Technology

11. Carl Denard (2008-2014): Assistant Professor, University of Florida, Gainesville

Postdoctoral Associates

1. Jung-kul Lee (2004-2006): Professor, Department of Chemical Engineering, Konkuk

University, Seoul, South Korea

2. Yoo Seong Choi (2006-2008): Assistant Professor, Department of Chemical Engineering,

Chungnam University, Seoul, South Korea

3. Levi Stanley (2010-2011): Assistant Professor, Department of Chemistry, Iowa State

University, Ames, IA

4. Xueyang Feng (2012-2014): Assistant Professor, Department of Biological Systems

Engineering, Virginia Tech, Blacksburg, VA

5. Han Xiao (2012-2015): Associate Professor, School of Life Sciences and Biotechnology,

Shanghai JiaoTong University, China

6. Meng Wang (2010-2015): Professor, Tianjin Institute of Industrial Biotechnology,

Tianjin, China

7. Xiaoyun Su (2012-2014): Associate Professor, Feed Research Institute Chinese Academy

of Agricultural Sciences, Beijing, China

8. Refaat Hamed (2013-2014): Assistant Professor, Faculty of Pharmacy, Assiut University,

Egypt

9. Hua Huang (2012-2014): Professor, School of Life Science, South China Normal

University, China

10. Shuobo Shi (2013-2017): Professor, Beijing University of Chemical Technology, China

11. Flora Liu (2013-2017): Associate Professor, Beijing University of Chemical Technology,

China

12. Yunfeng Hu (2011-2012): Professor, Chinese Academy of Science South China Sea

Institute, Guangzhou, China

13. Mingzi Zhang (2013-2018): Professor, National Health Research Institute, Taiwan

88

14. Yuan Qiao (2016-2017): Assistant Professor, Nanyang Technological University,

Singapore

Visiting Professors

1. Chun Li (2011): Professor, Department of Chemical Engineering, Beijing Institute of

Technology, Beijing, China

2. Yingxiao (Peter) Wang (2011-2012): Associate Professor, Department of Bioengineering,

University of California, San Diego

3. Yu Jiang (2011-2012): Associate Professor of Center for Industrial Biotechnology,

Chinese Academy of Sciences, Shanghai, China

4. Zhen Kang (2015-2016): Associate Professor, Jiangnan University, China

5. Xiao-wei Yu (2018-2019): Professor, Jiangnan University, China

Undergraduate Students

1. Edgar Goluch (2000-2003): Associate Professor, Department of Chemical Engineering,

Northeastern University, Boston, MA

i. personal history and professional...

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