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Contact your Alltech office or distributor for current or local prices.© Copyright 2004 Alltech Associates, Inc.

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IC SectionIC Columns .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..304-316

Introduction .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..305-306

Allsep™ Anion Columns .. .. .. .. .. .. .. .. .. .. .. 307

Novosep™ Anion Columns .. .. .. .. .. .. .. .. .. .. 308

Other Anion Columns .. .. .. .. .. .. .. .. .. ..309-310

Universal Cation Columns .. .. .. .. .. .. .. .. .. .. 311

Cation & Organic Acid Columns .. .. .. .. ..312-314

Transition Metal Columns .. .. .. .. .. .. .. .. .. .. 314

Surfactant Columns .. .. .. .. .. .. .. .. .. .. .. .. .. 315

Guard Cartridges .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 316

IC Accessories .. .. .. .. .. .. .. .. .. .. .. .. ..317-321

Starter Kits .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 317

IC SPE .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 318

Standards .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..319-320

EZ-LUTE™ Buffers .. .. .. .. .. .. .. .. .. .. .. .. .. .. 320

Other Accessories .. .. .. .. .. .. .. .. .. .. .. .. .. .. 321

IC Instruments .. .. .. .. .. .. .. .. .. .. .. .. ..322-327

Suppressors .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..322-324

Conductivity Detector .. .. .. .. .. .. .. .. .. .. .. .. 325

Column Heater .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 325

IC Detection Systems .. .. .. .. .. .. .. .. .. ..326-327


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IC Columns IntroductionI C C O L U M N S

AccessoriesKeep your system running smoothly with Alltech’s IC accessories, including certified standards, sample prep devices, and metal-free tubing and fittings.

InstrumentsEasily add IC detection to your existing HPLC equipment with Alltech’s patented Suppressors and our rugged, temperature controlled Conductivity Detector.

Ion ChromatographyAlltech has a complete line of products for Ion Chromatography

ColumnsWe manufacture a full line of high quality anion, cation, and specialty IC columns.

Tips for IC Column Selection

Particle Size - For common anion and cation separations, smaller particle size resins will result in better peak shapes and higher resolution than larger particle size resins. Larger particle sizes may offer better flow rates for some sample types.

Polymer vs. Silica - Polymer-based columns have higher capacities and broader pH stability. Silica-based columns give sharper peaks and overall better efficiency, but have a limited pH range and are not compatible with suppressor-based methods.

Suppressed vs. Non-Suppressed Detection - Most anion separations require suppression, while most cation separations do not. Consider whether a column has been optimized for suppressor-based detection, or is better suited to non-suppressed (single column) detection.

Column Length - For the same packing material, shorter columns provide faster run times, while longer columns provide better resolution. Always pick the packing that has the best selectivity for your sample, and then pick the column length based on the tradeoff between resolution and speed, taking into consideration the complexity of your sample and your desired run time.

Column i.d. - Narrower columns provide better mass sensitivity, accommodate smaller sample sizes, and significantly reduce solvent usage. Wider columns allow for larger sample sizes and minimize the negative effects of the system’s dead volume.

What is the difference between suppressed and non-suppressed detection?Suppressor-based detection is the best method for anion analysis. Some anion exchange mobile phases are highly conductive, and can interfere with conductivity detection – they introduce too much background to visualize the low-level anions in the sample. Mobile phase conductivity needs to be “suppressed” to efficiently detect sample anions at low concentrations. Suppressors allow the ppb-level sensitivity required for many EPA methods. Suppressors also let you use gradient elution for complex samples.

Non-suppressed detection is the best method for cation analysis. It is sometimes called Single Column Ion Chromatography (SCIC). It is also useful for anion exchange applications that do not require ppb-level sensitivity.

ApplicationsSee pages 514-521 for IC applications.

Contact your Alltech office or distributor for current or local prices.© Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices.© Copyright 2004 Alltech Associates, Inc.

306 307

IC Columns IntroductionI C C O L U M N S

IC Column Specifications

COLUMN COMPOSITION PH RANGE APPLICATIONSEPA

METHODS

OPTIMIZED FOR SUPPRESSED OR NON-SUPPRESSED

CONDUCTIVITY PAGE

Anion Exchange Columns

Allsep™ polymer-based anion exchanger, 7µm

pH 2-10 inorganic anions, weak and strong acid ions, metal complexes, organic acids

300.0, Part A

both 307

Allsep™ A-2 polymer-based anion exchanger, 7µm

pH 2-11 inorganic anions, organic acids, suitable for both weak and strong anions in a single run

300.0, Part A

both 307

Novosep™ A-1 polymer-based anion exchanger, 7µm

pH 2-11 inorganic anions, weak and strong acid anions; ideal for separation of hydrophobic anions

314.0 suppressed 308

Novosep™ A-2 polymer-based anion exchanger, 5µm

pH 3-12 inorganic anions and oxyhalides; ideal for separation of 7 common anions plus 3 oxyhalide anions in one run

300.0, 300.1, 317.0, 326.0

suppressed 308

Anion/S silica-based anion exchanger, 10µm

pH 2-5.5 inorganic anions non-suppressed 309

Anion/R polymer-based anion exchanger, 10µm

pH 2-12 inorganic anions, weak and strong acid ions

non-suppressed 309

PRP®-X100 polymer-based anion exchanger, 10µm

pH 1-13 inorganic anions, weak and strong acid ions

non-suppressed 310

AN1™ polymer-based anion exchanger, 9µm

pH 2-13 inorganic anions, weak and strong acid ions, organic acids

both 310

Cation Exchange Columns

Universal Cation

silica-based cation exchanger, 7µm

pH 2-7 groups I and II cations, amines, divalent transition metals

300.7 both 311

Universal Cation HR

silica-based cation exchanger, 3µm

pH 2-7 groups I and II cations, amines, divalent transition metals; smaller particle size for improved peak resolution

300.7 both 311

PRP®-X200 polymer-based cation exchanger, 10µm

pH 1-13 groups I and II cations (separate runs), amines, lanthanides

non-suppressed 312

Organic Acid Columns

PRP®-X300 polymer-based sulfonated cation exchanger

pH 1-13 simple aliphatic carboxylic acids and alcohols

non-suppressed 312

Prevail™ Organic Acid

silica-based reversed phase, 3µm and 5µm

pH 1-9 organic acids N/A 313

OA and IOA polymer-based cation exchanger, 6.5µm, 8µm, and 9µm

pH 1-14 organic acids, aromatic acids, sugars/organic acids/alcohols in one run; multiple particle sizes available

non-suppressed 313

Anion Exclusion polymer-based cation exchanger, 10µm

pH 1-13 organic acids, weak acid anions non-suppressed 314

Specialty Columns

Transition Metal

silica-based reversed phase, 3µm and 7µm

pH 2-7 high capacity, separates divalent transition metals and metal-cyano complexes more selectively than a cation exchange column

non-suppressed 314

Surfactant/R polymer-based reversed phase, 7µm

pH 1-14 anionic and cationic surfactants; separate short and long chain surfactants in one gradient run

both 315

Surfactant C8 silica-based reversed phase, 5µm

pH 2-7 short chain anionic surfactants non-suppressed 315

Surfactant C18 silica-based reversed phase, 5µm

pH 2-7 long chain or aromatic anionic surfactants

non-suppressed 315

Contact your Alltech offi ce or distributor for current or local prices.© Copyright 2004 Alltech Associates, Inc. Contact your Alltech offi ce or distributor for current or local prices.© Copyright 2004 Alltech Associates, Inc.

306 307

Column: Allsep™ A-2 Anion, 7µm, 4.6 x 100mm (Part No. 51219)Mobile Phase: 2.1mM NaHCO3/1.6mM Na2CO3

Flow Rate: 2.0mL/minDetector: Suppressed Conductivity

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Column: Allsep™ Anion, 7µm, 4.6 x 100mm (Part No. 51207)Mobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3


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1. Fluoride 2. Chloride 3. Nitrite 4. Bromide 5. Nitrate 6. Phosphate 7. Sulfate

1. Fluoride (1.0ppm) 2. Acetate (1.0ppm) 3. Formate (1.0ppm) 4. Chlorite (1.0ppm)

5. Chloride (2.0ppm) 6. Nitrite (2.0ppm) 7. Bromide (2.0ppm) 8. Chlorate (5.0ppm) 9. Nitrate (2.0ppm) 10. Phosphate (3.0ppm) 11. Sulfate (3.0ppm)

874713033

AnionsI C C O L U M N S

Allsep™ Anion IC Columns• Resolves fl uoride away from the water dip• Meets requirements for U.S. EPA Method 300.0 part A

The Allsep™ Anion Column is compatible with common IC mobile phases: carbonate, bicarbonate, p-hydroxybenzoic acid, phthalic acid, succinic acid, and sodium octane sulfonate.

Use this column for performing EPA Method 300.0 Part A “Determination of Inorganic Anions in Water” per U.S. EPA guidelines.

ALLSEP™ ANION COLUMNS

PACKING I.D. X LENGTHPEEK

PART NO.SS

PART NO. PRICE

Allsep™ Anion 4.6mm x 30 51217 512164.6mm x 50 51213 512144.6mm x 100 51207 512004.6mm x 150 51209 51208

ALLSEP™ A-2 ANION COLUMNS


PART NO.SS

PART NO. PRICE

Allsep™ A-2 4.6mm x 50 51221 512204.6mm x 100 51219 51218

Allsep™ A-2 Anion Specifi cationsComposition: Methacrylate based w/quaternary

alkanol amine functional groupsParticle Size: 7µmMobile Phase Limits: pH 2-11, 0-100% organic modifi er

Allsep™ Anion Specifi cationsComposition: Methacrylate based w/quaternary

ammonium functional groupsParticle Size: 7µmMobile Phase Limits: pH 2-10, 0-100% organic modifi er

Allsep™ A-2 Anion IC Columns• Separates both weak and strong acid anions in one

run • Resolves formate away from fl uoride and chloride

The Allsep™ A-2 Anion Column separates both weakly retained organic and strongly retained inorganic anions in one run. The high capacity Allsep™ A-2 Anion Column is also suitable for the separation of chlorite and chlorate.

Organic and Inorganic AnionsResolves Fluoride Awayfrom the Water Dip

GUARDS FOR ALLSEP™ AND ALLSEP™A-2 COLUMNSDESCRIPTION QTY. PART NO. PRICE GA-1 Anion Cartridges 3 38108All-Guard™ Cartridge Holder* ea 80101*Direct-Connect™ Column Coupler included .

Need an All-Guard™ holder? Save money with IC All-Guard™ Kits on page 316.

Alltech Anion Columns


308 309

Column: Novosep™ A-2 Anion, 5µm, 4.0 x 250mm (Part No. 51410)Mobile Phase: 3.6mM NaHCO3


1. Fluoride 2. Chlorite

101219299

1. Acetate (5ppm) 2. Chloride (1ppm)

Column: Novosep™A-1, 7µm, 4.6 x 100mm (Part No. 51406) Mobile Phase: A: Water B: 10mM Sodium Bicarbonate C: 10mM Sodium Carbonate with 1mM Cyanophenol Gradient: Time: 0 5 10 15 20 20.1 %B: 17 28 50 0 0 17 %C: 0 22 50 100 100 0 Flow Rate: 1.5mL/min Detector: Suppressed Conductivity

0 4

1 2

3 56 74

8 12 16 20 24 Min.

3. Phosphate (5ppm) 4. Sulfate (1ppm) 5. Oxalate (5ppm) 6. Perchlorate (5ppm) 7. Citrate (5ppm)

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3. Bromate 4. Chloride 5. Nitrite-N 6. Bromide 7. Chlorate 8. Dichloroacetate 9. Nitrate-N 10. Phosphate-P 11. Sulfate


Anions in High Ionic Strength Water by EPA Method 300.0

Novosep™ A-1 Anion SpecificationsComposition: Methacrylate based w/quaternary

ammonium functional groupsParticle Size: 7µmMobile Phase Limits: pH 2-11, 0-100% organic modifier

(methanol, acetonitrile, acetone)

Novosep™ A-2 Anion SpecificationsComposition: Polyvinyl alcohol based w/quaternary

ammonium functional groupsParticle Size: 5µmMobile Phase Limits: pH 3-12, 0-100% organic modifier, 10%

modifier is practical upper limit

Novosep™ A-2 Anion IC Columns• Separates seven inorganic anions and three oxyhalide

anions in a single run• Meets the requirements of U.S. EPA Methods 300.0,

300.1, 317.0, and 326.0

The Novosep™ A-2 Anion Column is ideal for the separation of 10 inorganic anions by suppressor-based ion chromatography using U.S. EPA Method 300.1. The column can also be used to determine inorganic anions in drinking water per U.S. EPA Method 300.0 and trace bromate in drinking water using U.S. EPA Methods 317.0 and 326.0.

Novosep™ A-1 Anion IC Columns• Separates common anions and strongly retained

hydrophobic anions• Meets the requirements of U.S. EPA Method 314.0

The Novosep™ A-1 Column is your best choice for routine anion analysis and for determining other strongly retained hydrophobic anions that previously required specialty columns.

The Novosep™ A-1 Column can simultaneously separate weakly and strongly retained anions by gradient elution. Carbonate/bicarbonate gradients offer a powerful tool for separating anions that have a wide range of affinities for the column.

Weakly and Strongly Retained Anions

NOVOSEP™ A-1 ANION COLUMNS


PART NO.SS

PART NO. PRICE

Novosep™ A-1 4.6mm x 30 51401 514024.6mm x 50 51403 514044.6mm x 100 51405 514064.6mm x 150 51407 51408

NOVOSEP™ A-2 ANION COLUMN


PART NO. PRICE

Novosep™ A-2 4.0mm x 250 51410

GUARDS FOR NOVOSEP™ AND NOVOSEP™ A-2 ANION COLUMNS DESCRIPTION QTY. PART NO. PRICE GA-1 Anion Cartridges 3 38108All-Guard™ Cartridge Holder* ea 80101*Direct-Connect™ Column Coupler included.



308 309

Column: Anion/S, 10µm, 4.6 x 100mm (Part No. 269012)Mobile Phase: 1.5mM Sulfuric AcidFlow Rate: 1.0mL/minDetector: UV at 210nm

1. Nitrite 2. EDTA 3. Nitrate 4. Bromide

8284

Column: Anion/R, 10µm, 4.1 x 250mm (Part No. 269029)Mobile Phase: 4mM Sodium Hydroxide/ 0.1mM Sodium BenzoateFlow Rate: 1.5mL/minDetector: Conductivity

1. Borate (5ppm) 2. Silicate (10ppm) 3. Formate (10ppm) 4. Sulfi de (10ppm) 5. Chloride (20ppm) 6. Cyanide (20ppm)

5697

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Anion/S IC Columns• Silica-based for symmetrical peak shapes• Separates inorganic and organic anions

The Anion/S Columns are best suited for routine separations of chloride, bromide, nitrate, and sulfate. Not suitable for fl uoride analysis.

ANION/S SPECIFICATIONS

Composition: Silica w/quaternary ammonium ion exchanger

Particle Size: 10µmExchange Capacity: 0.25meq/gMobile Phase Limits: pH 2-5.5, 0-100% organic modifi er

Weak Acid Anions

ANION/R SPECIFICATIONS

Composition: Poly(styrene-divinylbenzene)Trimethylammonium

Particle Size: 10µmExchange Capacity: 0.19 ± 0.02meq/gMobile Phase Limits: pH 2-12, 0-100% organic modifi er

Anion/R IC Columns• Polymer-based for broad pH stability• Separates seven common inorganic anions

Because of their high pH stability, these column are ideal for the separation of anions using high pH mobile phases such as p-hydroxybenzoate, sodium hydroxide, and sodium carbonate/bicarbonate. The larger dimension columns (150mm and 250mm) are suitable for a high resolution separation of the seven common inorganic anions.

EDTA andOther Anions

ANION/S COLUMNS


PART NO.SS

PART NO. PRICE

Anion/S 4.6mm x 100 269012 2690134.6mm x 250 269011 269001

ANION/R COLUMNS


PART NO.SS

PART NO. PRICE

Anion/R 4.1mm x 100 – 2690314.1mm x 250 – 2690294.6mm x 100 269036 –4.6mm x 150 269034 –

GUARDS FOR ANION/S AND ANION/R COLUMNSDESCRIPTION QTY. PART NO. PRICE GA-1 Anion Cartridges 3 38108All-Guard™ Cartridge Holder* ea 80101*Direct-Connect™ Column Coupler included.




310 311

Column: PRP®-X100, 10µm, 4.1 x 150mm (Part No. 79434)Mobile Phase: 0.003N Sulfuric AcidFlow Rate: 2mL/minDetector: UV at 254nm

1. CuEDTA

8068

Column: IC Sep AN1™, 9µm, 4.6 x 250mm (Part No. 38140)Mobile Phase: 1.8mM NaHCO3, 1.8mM Na2CO3


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1. Fluoride 2. Chloride 3. Nitrite 4. Bromide

6316 5. Nitrate 6. Phosphate 7. Sulfite 8. Sulfate


EDTA in Eye DropsSeparation of Sulfite and Sulfate

IC Sep AN1™ SpecificationsComposition: Poly(styrene-divinylbenzene) alkyl

dimethyl ethanol ammonium functional group

Particle Size: 9µmExchange Capacity: 0.05meq/gMobile Phase Limits: pH 2-13, No organic solvents

Hamilton PRP®-X100 SpecificationsComposition: Trimethylammonium psDVB copolymerParticle Size: 10µm, sphericalExchange Capacity: 0.19meq/g Mobile Phase Limits: pH 1-13, 0-100% organic modifier

Hamilton PRP®-X100 IC Columns

High pH stability makes this column ideal for the separation of weak anions. PRP®-X100 provides excellent resolution for the seven most frequently analyzed inorganic anions. For non-suppressed analyses only.

IC Sep AN1™ IC Column• Separates anions with carbonate/bicarbonate and

hydroxide mobile phases

The IC Sep AN1™ Column is compatible with both suppressor-based and non-suppressed detection. A variety of mobile phases including sodium carbonate/bicarbonate, sodium hydroxide, and borate/gluconate may be used.

IC SEP AN1™ COLUMNSPACKING I.D. X LENGTH PART NO. PRICE

AN1™ 4.6mm x 250 38140

HAMILTON PRP®-X100PACKING I.D. X LENGTH PART NO. PRICE

PRP® X-100 2.1mm x 150 793482.1mm x 250 793464.1mm x 100 794394.1mm x 150 794344.1mm x 250 79433

Anion Columns

HAMILTON GUARD KITS AND CARTRIDGESDESCRIPTION QTY PART NO. PRICE

Guard Column Starter KitsPRP®-X100, 1 Holder, 2 Cartridges ea 79448Replacement Guard Cartridges(Requires Hamilton Guard Holder)PRP®-X100, 25 x 2.3mm 5 79446

IC SEP GUARDSDESCRIPTION QTY PART NO. PRICE

Guard Column, 50 x 4.6mm ea 38145Guard Column Guard-Disc® 5 38146

Choose a guard column or integral guard discs to protect your IC Sep Column.


310 311

Column: Universal Cation, 7µm, 4.6 x 100mm (Part No. 27106)Mobile Phase: 2mM Tartaric Acid/1mM Oxalic AcidFlow Rate: 1.0mL/minDetector: Conductivity

1. Lithium (0.2ppm) 2. Sodium (1.5ppm) 3. Ammonium (1.5ppm) 4. Potassium (2.5ppm)

10318Column: Universal Cation, 7µm, 4.6 x 100mm (Part No. 27106)Mobile Phase: 3mM Methanesulfonic AcidFlow Rate: 1.0mL/minDetector: Conductivity

1. Lithium (0.12ppm) 2. Sodium (0.90ppm) 3. Ammonium (0.90ppm)

13035

Column: Universal Cation HR, 3µm, 7.0 x 53mm (Part No. 23109)Mobile Phase: 3mM Methanesulfonic AcidFlow Rate: 2.5mL/minDetector: Conductivity

13013

0 1

1

2

2

3

3

4

4

5

5

6

6

7 8 9 Min.

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5. Nickel (2.5ppm) 6. Zinc (2.5ppm) 7. Cobalt (2.5ppm) 8. Magnesium (2.0ppm) 9. Calcium (2.0ppm)

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4. Potassium (1.5ppm) 5. Cesium (0.8ppm) 6. Magnesium (1.2ppm) 7. Calcium (1.2ppm) 8. Strontium (1.6ppm)

1. Lithium 2. Sodium 3. Ammonium 4. Potassium 5. Magnesium 6. Calcium

CationsI C C O L U M N S

Metal Ions

Universal Cation Specifi cationsComposition: Silica coated with polybutadiene/maleic

acid copolymerParticle Size: 3µm and 7µm, spherical Mobile Phase Limits: pH 2-7, 0-100% organic modifi er

• Separate Groups I and II cations in one isocratic run• Separate transition metals without post-column

reaction• New Universal Cation HR 3µm particle size for

improved effi ciency

Compatible with a variety of mobile phases including complexing acids (citric acid, tartaric acid, oxalic acid), mineral acids (nitric acid, hydrochloric acid, sulfuric acid) and non-complexing organic acids (methanesulfonic acid).

Cesium and Strontium

Rapid Analysis of Monovalentand Divalent Cations

UNIVERSAL CATION COLUMNS


PART NO.SS

PART NO. PRICE

7µm 4.6mm x 100mm 27106 27100

3µm HR 4.6 x 100mm – 231007.0 x 53mm – 23109

Alltech Cation ColumnsUniversal Cation and Universal Cation HR IC Columns

GUARDS FOR UNIVERSAL CATION COLUMNS DESCRIPTION QTY. PART NO. PRICE GC-2 Universal Cation 3 271010GC-3 Universal Cation HR 3 23110All-Guard™ Cartridge Holder* ea 80101*Direct-Connect™ Column Coupler Included.



312

Column: PRP®-X300, 7µm, 4.1 x 150mmMobile Phase: 1.5mN Sulfuric Acid: Acetonitrile (92:8)Flow Rate: 0.5mL/minDetector: UV at 210nm

1. Maleic Acid (1160ppm) 2. Malonic Acid (2000ppm) 3. Succinic Acid (210ppm) 4. Fumaric Acid (12.3ppm) 5. Endothall (9080ppm)

9146

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Column: PRP®-X200, 10µm, 4.1 x 150mm (Part No. 79441)Mobile Phase: 0.004N HNO3 in 30% MeOHFlow Rate: 2mL/minDetector: Conductivity

1. Lithium 2. Sodium 3. Ammonium 4. Potassium 5. Cesium

5204

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Cations and Organic AcidsI C C O L U M N S

Hamilton PRP®-X200 Cation Analysis Columns

For separation of the alkali metal and alkaline earth cations in separate runs.

Hamilton PRP®-X300 Organic Acid Columns

For separation of simple aliphatic carboxylic acids and alcohols. Inorganic anions are not retained.

HAMILTON PRP®-X300 COLUMNSPARTICLE SIZE I.D. X LENGTH PART NO. PRICE

PRP®-X300, 3um 4.1 x 150mm 79819PRP®-X300, 7um 4.1 x 150mm 79464

4.1 x 250mm 794654.6 x 150mm 79485

HAMILTON PRP®-X200 COLUMNSPARTICLE SIZE I.D. X LENGTH PART NO. PRICE

PRP®-X200 2.1 x 150mm 793942.1 x 250mm 793474.1 x 150mm 794414.1 x 250mm 79442

Hamilton PRP®-X200 SpecificationsComposition: Sulfonated psDVB copolymerParticle Size: 10µm, sphericalExchange Capacity: 35 umeq/gMobile Phase Limits: pH 1-13, 0-100% organic modifier

Hamilton PRP®-X300 SpecificationsComposition: Sulfonated psDVB in H+ formParticle Size: 3µm and 7µmMobile Phase Limits: pH 1-13, 0-100% organic modifier

Alkali Metals Endothall

Hamilton Cation & Organic Acid Columns

HAMILTON PRP®-X200 GUARD KITS AND CARTRIDGESDESCRIPTION QTY PART NO. PRICE

Guard Column Starter KitPRP®-X200, 1 Holder, 2 Cartridges

ea 79456

Replacement Guard Cartridges(Requires Hamilton Guard Holder)PRP®-X200, 25 x 2.3mm

5 79449

HAMILTON PRP®-X300 GUARD KITS AND CARTRIDGESDESCRIPTION QTY PART NO. PRICE

Guard Column Starter KitPRP®-X300, 1 Holder, 2 Cartridges

ea 79460

Replacement Guard Cartridges(Requires Hamilton Guard Holder)PRP®-X300, 25 x 2.3mm

5 79453


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Prevail™ OA• Modified silica for higher flow rates and broader pH

stability• Acid-stable for long column lifetimes• Does not require a column heater

Prevail™ OA (Organic Acid) Columns separate organic acids with high efficiency, sharp peaks, and short run times. Use pH adjustments to adjust the selectivity for di-and tri-carboxylic acids.

Column: Prevail™ Organic Acid, 5µm, 4.6 x 150mm (Part No. 88640)Mobile Phase: 25mM KH2PO4, pH2.5Flow Rate: 1.5mL/minDetector: UV at 210nm

Organic Acids

9384

1. Oxalic Acid 2. Tartaric Acid 3. Formic Acid 4. Malic Acid

PREVAIL™ ORGANIC ACID COLUMNSPACKING I.D. X LENGTH PART NO. PRICE

Prevail™ OA, 3µm 4.6 x 100mm 886504.6 x 150mm 88655

Prevail™ OA, 5µm 4.6 x 150mm 886404.6 x 250mm 88645

ALLTECH ORGANIC ACID COLUMNSPACKING I.D. X LENGTH PART NO. PRICE

OA-1000, 9µm 6.5 x 300mm 9046OA-2000, 6.5µm 6.5 x 100mm 9048IOA-1000, 9µm 7.8 x 300mm 9646IOA-2000, 8µm 6.5 x 150mm 9648

Wine Analysis by High ResolutionIon Exclusion

5411

Alltech OA and IOA• Analyze organic acids and alcohols in a single run• Polymer-based for broad pH stability

OA: selective for aliphatic and aromatic acids

Use OA-1000 for inorganic anions and most organic acids. Use OA-2000 for low pKa organic acids, low molecular weight straight chain acids, and aromatic acids.

IOA: separate short chain organic acids from fructose and glucose

Use IOA-1000 for TCA or Krebs cycle acids, carbohydrates, and alcohols. Use IOA-2000 for fast separation of acids and some alcohols.

Column: IOA-1000, 9µm, 7.8 x 300mm (Part No. 9646)Mobile Phase: 0.005N Sulfuric AcidFlow Rate: 0.3mL/minColumn Temp: 60°CDetector: RI

5. Lactic Acid 6. Acetic Acid 7. Diglycollic Acid 8. Maleic Acid 9. Citric Acid 10. Succinic Acid 11. Fumaric Acid

PREVAIL™ ORGANIC ACID ALL-GUARD™ CARTRIDGESDESCRIPTION QTY. PART NO. PRICE Prevail™ OA (holder required) 3 96429All-Guard™ Cartridge Holder* ea 80101*Direct-Connect™ Column Coupler included.

GUARDS FOR OA AND IOA COLUMNSDESCRIPTION QTY. PART NO. PRICE Organic Acid Guard Cartridge Kit Includes: 1 Holder, 2 Cartridges

ea 28883

Organic Acid Cartridge, 24 x 4.0mm 2 28884

OA and IOA SpecificationsComposition: Highly sulfonated poly (styrene-

divinylbenzene) cation exchangerParticle Size: 6.5µm, 8µm, and 9µmMobile Phase Limits: pH 1-14, isocratic aqueous mobile phases

only; no organics

Prevail™ OA SpecificationsComposition: Silica base, with specially modified

reversed-phase functional groupsParticle Size: 3µm and 5µmMobile Phase Limits: pH 1-9, 0-100% organic modifier

I C C O L U M N S

Organic Acids

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Alltech Organic Acid Columns

1. Citric Acid 2. Tartaric Acid 3. Glucose 4. Malic Acid 5. Fructose 6. Acetic Acid 7. Glycerol 8. Lactic Acid 9. Methanol 10. Ethanol


314 315

Transition MetalAlltech Transition Metal Columns

• Separate divalent transition metals and metal-cyano complexes more selectively than a cation exchange column

To separate divalent transition metals, use conductivity detection for concentrations as low as 1ppm, or UV detection with post-column reaction for ppb-level concentrations

To separate metal-cyano complexes, use Alltech’s low UV IPC A (Part No. 185149) as the ion-pair reagent, and methanol or acetonitrile as the organic modifi er.

TRANSITION METAL COLUMNSPACKING I.D. X LENGTH PEEK PART NO. PRICE

C18, 3µm 4.6 x 100mm 269064C18, 7µm 4.6 x 250mm 269067

Column: Transition Metal C18, 7µm, 250 x 4.6mm (Part No. 269067)Mobile Phase: 0.005M Tetrabutylammonium Sulfate (Preformulated Alltech Low UV IPC A, Part No. 185149) in 30% AcetonitrileFlow rate: 0.7mL/minDetector: UV at 214nm

1. Fe(CN)64- 2. Ag(CN)2- 3. Fe(CN)63- 4. Ni(CN)42- 5. Au(CN)2-

Metal-Cyano Complexes

5358

ANION EXCLUSION COLUMNS


PART NO.SS

PART NO. PRICE

Anion Exclusion, 10µm 7.5 x 100mm 269069 —7.5 x 300mm 269062 —7.8 x 100mm — 2690687.8 x 300mm — 269006

Column: Anion Exclusion, 10µm, 300 x 7.5mm (Part No. 269062)Mobile Phase: 1.5mM Sulfuric AcidFlow rate: 0.4mL/minDetector: Conductivity

Organic Acid Standards

13037

Anion ExclusionAlltech Anion Exclusion Columns

• Separate organic acids and weak acid anions• Polymer-based for broad pH stability• Economical choice for small inorganics

Anion Exclusion Columns separate organic acids and weakly ionized anions by an anion exclusion mechanism. Dilute mineral acids are the typical mobile phases for the separation. Acetonitrile may be used as an organic modifi er to decrease the retention of hydrophobic compounds.

1. Oxalic Acid 2. Maleic Acid 3. Malic Acid 4. Succinic Acid 5. Formic Acid 6. Acetic Acid

Transition MetalsI C C O L U M N S

Anion Exclusion Specifi cationsComposition: Highly sulfonated poly (styrene-

divinylbenzene) cation exchangerParticle Size: 10µmMobile Phase Limits: <10% acetonitrile, no methanol

<5% isopropyl or ethyl alcohols

Transition Metal Specifi cationsComposition: Silica-based with C18 bonded phase

(20% Carbon)Particle Size: 3µm and 7µm, spherical Mobile Phase Limits: pH 2-7

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TRANSITION METAL ALL-GUARD™ CARTRIDGESDESCRIPTION QTY. PART NO. PRICE GRP-2 Revised Phase 3 38129All-Guard™ Cartridge Holder* ea 80101*Direct-Connect™ Column Coupler included.

ANION EXCLUSION ALL-GUARD™ CARTRIDGESDESCRIPTION QTY. PART NO. PRICE Anion Exclusion GIE-1 3 38118All-Guard™ Cartridge Holder* ea 80101*Direct-Connect™ Column Coupler included.



314 315

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Surfactant/R IC Columns• Separate anionic and cationic surfactants• Separate short and long chain surfactants in one

gradient run

The Surfactant/R Column separates anionic and cationic surfactants by ion-pair chromatography along with suppressed conductivity detection. This column can also be used for other reversed-phase applications.

1. Tetramethyl Ammonium (20ppm) 2. Tetraethyl Ammonium (20ppm) 3. Tetrapropyl Ammonium (20ppm) 4. Tetrabutyl Ammonium (30ppm) 5. Dodecyltrimethyl Ammonium (30ppm) 6. Cetyltrimethyl Ammonium (40ppm) 7. Myristyltrimethyl Ammonium (50ppm)

Short and Long Chain Cationic Surfactants

10317

Column: Surfactant C8, 5µm, 4.6 x 250mm (Part No. 269072)Mobile Phase: 0.02g/L Ammonium Acetate in 40% MethanolFlow rate: 1.2mL/minDetector: Conductivity

Alkyl Sulfates

5998

Surfactant/C IC Columns• For non-suppressed analyses• Silica-based for symmetrical peak shapes

Choose Surfactant C8 for short chain anionic surfactants such as alkyl sulfates and alkyl sulfonates.

Choose Surfactant C18 for long chain or aromatic surfactants such as xylene sulfonate and dodecyl benzene sulfonate.

1. Octyl Sulfate 2. Decyl Sulfate 3. Dodecyl Sulfate

Column: Surfactant/R Column, 7µm, 4.6 x 150mm (Part No. 25101)Mobile Phase: A: 2mM Nonafl uoropentanoic Acid B: 100% AcetonitrileGradient: Time: 0 5 8 20 %B: 30 30 35 80Flow rate: 1.0mL/minDetector: Suppressed Conductivity

I C C O L U M N S

Surfactants

SURFACTANT/R COLUMNS


PART NO.SS

PART NO. PRICE

Surfactant/R, 7µm 4.6 x 150mm 25101 25100

GUARDS FOR SURFACTANT/R COLUMNSDESCRIPTION QTY. PART NO. PRICE GRP-1 Revised Phase 3 38124All-Guard™ Cartridge Holder* ea 80101*Direct-Connect™ Column Coupler included.

SURFACTANT/C COLUMNS


PART NO.SS

PART NO. PRICE

C8, 5µm 4.6 x 250mm 269072 269092C18, 5µm 4.6 x 250mm 269071 269091

GUARDS FOR SURFACTANT/C COLUMNSDESCRIPTION QTY. PART NO. PRICE GRP-2 Revised Phase 3 38129All-Guard™ Cartridge Holder* ea 80101*Direct-Connect™ Column Coupler included.

Surfactant/R Specifi cationsComposition: Polydivinylbenzene (DVB) based resinParticle Size: 7µm Mobile Phase Limits: pH 1-14, 0-100% organic modifi er

Surfactant/C Specifi cationsComposition: Silica-based C8 or C18Particle Size: 5µm Mobile Phase Limits: pH 2-7, 0-100% organic modifi er

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Alltech Surfactant Columns



316 317

IC Guard Cartridges• Convenient - change cartridges in seconds without

tools• No connecting tubing required• All-Guard™ Holders work with other manufacturer’s

columns

ALL-GUARD™ KITSDESCRIPTION QTY. PART NO. PRICE

All-Guard™ Kits (Includes 3 PEEK Cartridges, 1 Holder, and 1 Direct-Connect™ Column Coupler)GA-1 Anion ea 38109GC-1 Cation ea 38113GC-2 Universal Cation ea 27109GC-3 Universal Cation HR ea 23115GIE-1 Anion Exclusion ea 38117GRP-1 Resin Reversed-Phase ea 38122GRP-2 Silica Reversed-Phase ea 38123Guard Holder (Includes Direct-Connect™ Column Coupler) All-Guard™ Cartridge Holder ea 80101Replacement Column Coupler ea 28191

Guard Reference Table

COMPANY COLUMN TYPECARTRIDGE

TYPEALL-GUARD™

KIT PART NO.Alltech Allsep™, Allsep™ A-2 GA-1 38109

Novosep™A-1 GA-1 38109Novosep™ A-2 GA-1 38109Anion/R, Anion/S GA-1 38109Universal Cation GC-2 27109Universal Cation HR GC-3 23115Anion Exclusion GIE-1 38117Transition Metal C18 GRP-2 38123Surfactant/R GRP-1 38122Surfactant C8, C18 GRP-2 38123

Dionex IonPac™ AS4, AS4A, AS4A-SC, AS7, AS9, AS10, AS11, AS12, AS14

GA-1 38109

IonPac™ CS3, CS10, CS11 GC-1 38113IonPac™ CS12, CS14, CS15 GC-2 27109IonPac™ NS1 GRP-1 38122IonPac™ AS1, AS5 GIE-1 38117

Waters™

IC-Pak™-A GA-1 38109IC-Pak™-M/D GC-2 27109Ion Exclusion GIE-1 38117µBondapak™ C18 GRP-2 38123Radial-Pak™ C18 GRP-2 38123Nova-Pak™ C18 GRP-2 38123

Hamilton PRP®-X100 GA-1 38109PRP®-X200 GC-1 38113PRP®-X300 GIE-1 38117PRP®-1 GRP-1 38122

Misc. Sarasep ANIONSEP AN1™ GA-1 38109Bio-Rad®, Aminex®, HPX-87 GIE-1 38117Interaction® ION-120 GA-1 38109Interaction® ION-210 GC-1 38113Interaction® ORH-801 GIE-1 38117Metrohm Metrosep GA-1 38109Metrohm SUPER SEP™ GC-2 27109

Inert, metal-free PEEK fluid path

Guard CartridgesI C C O L U M N S

Protect your IC columns from contaminants with All-Guard™ Cartridges. The All-Guard™ System’s unique holder is open on the side for fast and easy installation of cartridges.

Save money with IC Guard Cartridge Starter Kits, including an All-Guard™ Holder and three guard cartridges. Connect the All-Guard™ Holder directly to your analytical column using Alltech’s patented Direct-Connect™ Column Coupler, included with each holder.

5419

REPLACEMENT CARTRIDGESDESCRIPTION QTY. PART NO. PRICE

All-Guard™ Replacement Cartridges, PEEK GA-1 Anion 3 38108GC-1 Cation 3 38114GC-2 Universal Cation 3 271010GC-3 Universal Cation HR 3 23110GIE-1 Anion Exclusion 3 38118GRP-1 Resin Reversed-Phase 3 38124GRP-2 Silica Reversed-Phase 3 38129

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