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Ideal set upIdeal set upof Healthof Health

Facility for TBFacility for TB

MicroscopyMicroscopy

ServiceService

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MICROSCOPY CENTERout door sputum

collection area

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Collection Area for Sputum SampleCollection Area for Sputum Sample

A designated area

wherein PRIACYofPATIENT is

considered

-Awa! from patientwaiting area or

passer"#!

-$ell" %entilated

-Not totall! enclosed

-$ith shade &plastic

roofing is preferred'

natural sterili(ation)

to protect from rain*

C+,"Central -u(on

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Collection Area for Sputum SampleCollection Area for Sputum Sample

-sin. with

running water

&preferred) for

hand washing

-presence of

soap for good

h!giene" poster/ hand

washing

instruction

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Collection Area for Sputum SampleCollection Area for Sputum Sample

-with signage

0Collection Area10Collection Area1

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Improper set"upImproper set"up

-without

enough

shade and

located#eside the

air"con

e2haust

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MICROSCOPY CENTER

-A3ORATORY SET"4Pfor A53 microscop!

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1.1. well-ventilatedwell-ventilated

2.2. enough space at least 4 x 4 sq.meterenough space at least 4 x 4 sq.meter

for laboratory microscopy workfor laboratory microscopy work3.3. continuous supply of electricity andcontinuous supply of electricity and

4.4. tap water must be provided as welltap water must be provided as well

-A3ORATORY ROOM AREA-A3ORATORY ROOM AREAshould beshould be!!

-A3ORATORY SET"4P-A3ORATORY SET"4P

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Set"up of a microscop! la#orator!Set"up of a microscop! la#orator!

and 6ualit! Assurance Centerand 6ualit! Assurance Center77

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1"1" receiving areareceiving area

2)2) smearing areasmearing area3"3" staining areastaining area

4"4" microscopy areamicroscopy area

#"#" writing areawriting area$"$" releasing areareleasing area

%"%" storage spacestorage space

-A3ORATORY ROOM ARRAN8EMENT7-A3ORATORY ROOM ARRAN8EMENT7&deally a '( microscopy center should at least&deally a '( microscopy center should at least

consist of the following sections!consist of the following sections!

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-A3ORATORY ROOM SECTIONS

and its 54NCTIONS

9* RECEPTION area for recei%ing the Specimen

:* SMEAR PREPARATIONarea

) well ventillated or with nearby

installed exhaust fan "

for smear preparation and heatfi2ed smear

;* STAININ8 area for staining smears

* STORA8E SPACE for stain reagent ? other la#supplies

@* RE-EASIN8 area for the releasing results

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9* NTP -a#orator!

Register

:* 3allpen;* Sputum cup

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9*9* Ordinar! pen or pencil forOrdinar! pen or pencil for

la#elingla#eling

:*:* Alcohol lampAlcohol lamp

;*;* 5rosted glass slides5rostedglass slides

* $ashing #ottle$ashing #ottle

Smear Preparation Area7Smear Preparation Area7

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9* A53 stain

reagents

:* Staining #ridge

;* 5orcep

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Sin. for Staining A53 SmearsSin. for Staining A53 Smears

-deep sin. is preferred )at least 1 foot deep"

-dar. colored tile is preferred than stainless

&easil! corrodes)

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1.*lectric (inocular microscope

2.+il immersion ),argill 'ype "

3.2 oplin /ars with xylene4.0appe

#.ens paper

$.ry cotton

%.(allpen-black and red color.lide box

The Materials & equipment inThe Materials & equipment in

AFB microscopy area:AFB microscopy area:

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Microscop! SectionMicroscop! Section

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9* A53 reagents

:* Sputum cups

;* NTP 5orms

BNTP la#orator! registerB-a# reuest form

BNTP uarterl! report formD

and

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9* -a#orator! reuest form

:* NTP la#orator! register

;* NTP uarterl! report forms

and #allpen

RECOR,IN8 AREA ? RE-EASIN8RECOR,IN8 AREA ? RE-EASIN8

O5 RES4-TS7O5 RES4-TS7

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-A3ORATORY SET"4P

An ideal laboratory set-up that isAn ideal laboratory set-up that isclean, dust-free, well-organizedclean, dust-free, well-organized

and well-equipped one createsand well-equipped one creates

convenient workingconvenient working

environmentenvironment

As well as facilitates processing ofAs well as facilitates processing of

AF !mear "icroscopyAF !mear "icroscopy

#$amination easily and accurately#$amination easily and accurately

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Set"up of a microscop! la#orator!Set"up of a microscop! la#orator!

and 6ualit! Assurance Centerand 6ualit! Assurance Center77