Identification of Root Rot Resistant Germplasm for Mozambique and Zambia

Carlos A. Urrea, Celestina Jochua, Kennedy Muimui, James Steadman, Suzana Fernandes, Graciela Godoy-

Lutz1, Chikoti Mukuma, Kent Eskridge, Eduardo Valentín Cruzado, and Marcial Pastor-Corrales

Objective 2. Identify sources of host resistance to RR/CR pathogens

Main source of germplasm: 1. Andean diversity Panel (

evaluated for drought tolerance, ALS, Anthracnose and Nitrogen Fixation)

2. With addition of 50 Middle American/Andean selected improved lines from the Nebraska Dry Bean Breeding Program

ZAMBIA AND MOZAMBIQUE

2013 Experiments

Zambia and Mozambique at 2 locations= 357 and 352 entries, respectively

Nebraska (Andean BeanCAP Panel and ADP)= 49 and 81 entries, respectively Terminal drought CBB at North Platte Cooking Time

2014 Experiments (Mozambique, Zambia, and Nebraska)

66 entries arranged in Split plot design: Main Plot= Growth Habit (Ind./Det.)

Subplot= Genotypes 23 ADP + 23 UNL + 4 local checks (Det.) 21 ADP + 7 UNL + 2 local checks (Ind.)

2014 Experiments

Nebraska CBB screening at North Platte Drought vs non-drought stress at

Scottsbluff, NE Terminal drought

Cooking time

Common Bacterial Blight at North Platte, NE

CBB at the WREC, North Platte, NE in 2014

Cooking Test

Seeds were soaked overnight (16 hours) and placed in the Mattson Bean Cooker. Beans are cooked when 80% of the weighted plungers drop.

Drought experiments at Scottsbluff, NE in 2014

Root rot collection samples at Scottsbluff, NE

Western Nebraska Survey 2013-2014

Selected Lines, 2014 Nebraska

CODE NAME GM PR% CBB kg ha-1

% (1-9)

NE34-12-47 NE34-12-47 3105 32.7 3.4 NE34-12-45 NE34-12-45 2822 22.2 5.9 NE34-12-34 NE34-12-34 2641 19.6 6.4 NE34-12-28 NE34-12-28 2362 28.3 6.9 NE34-12-50 NE34-12-50 1929 20.6 2.9 ADP-255 G 10994 1924 47.5 3.9 ADP-14 KIANGWE 1825 11.8 5.4 ADP-517 Carioca, Kihala 1755 13.4 7.4 ADP-654 USDK-4 1359 26.4 5.4 ADP-680 Clouseau 1350 7.9 5.9 ADP-27 Incomparable 1300 35.1 5.6 ADP-21 MBULAMTWE 1212 7.1 4.9 ADP-19 KASUKANYWELE 1115 15.5 5.4 ADP-442 Larga Comercial 1006 30.8 6.4 ADP-188 G 1375 412 76.6 5.9 GRAND MEAN 1841 25.9 5.4

2014 Mid-term Review

2015 African trials: RCBD Split Plot Effect of fertilization/non-fertilization Control/non-control of bean stem maggot

ANOVA, Misamfu, Zambia 2015

Root Rot

Source DF Type III SS Mean Square

F Value Pr > F

entry 15 0.84 0.06 0.71 0.77

fert 1 0.07 0.07 0.93 0.34

entry*fert 15 1.39 0.09 1.17 0.30

insect 1 0.09 0.09 1.19 0.28

entry*insect 15 1.37 0.09 1.16 0.31

fert*insect 1 0.00 0.00 0.00 0.95

entry*fert*insect 15 0.79 0.05 0.67 0.81

ANOVA, Misamfu, Zambia 2015

Yield Plot Source DF Type III SS Mean Square F Value Pr > F

entry 15 599933.14 39995.54 5.13 <.0001

Fert 1 18219.85 18219.85 2.34 0.13

entry*fert 15 123677.62 8245.17 1.06 0.40

insect 1 46381.84 46381.84 5.95 0.02

entry*insect 15 80567.03 5371.14 0.69 0.79

fert*insect 1 4545.25 4545.25 0.58 0.45

entry*fert*insect 15 34894.05 2326.27 0.30 0.99

Chokwe, Mozambique 2015

Source DF F Value RR1 Pr > F RR1

F Value RR2

Pr > F RR2

entry 15 2.99 0.0004 1.43 0.1443

fert 1 14.42 0.0002

1 0.3192

entry*fert 15 1.38 0.1656

0.74 0.7358

insect 1 1.41 0.2375

0.36 0.5496

entry*insect 15 1.05 0.4117 0.79 0.6903

fert*insect 1 0.51 0.4777

1 0.3192

entry*fert*insect 15 1.89 0.0299

1.26

0.2398

Chokwe, Mozambique 2015 emerg rootr1 rootr2 als ant cbb rust bcmv

KIANGWE 47.6 0.2 0.0 0.0 0.0 0.0 0.0 0.5 KASUKANYWELE 42.2 0.5 0.2 0.0 0.0 0.1 0.1 0.0 Uyole 96 47.4 0.3 0.1 0.0 0.0 0.0 0.0 0.1 G 1375 47.4 0.7 0.0 0.0 0.0 0.8 0.0 0.7 G 10994 45.8 0.8 0.3 0.0 0.0 0.0 1.3 0.0 G 22246 46.3 0.4 0.1 0.0 0.0 0.0 0.0 0.1 Larga Comercial 47.1 0.1 0.0 0.0 0.0 0.0 0.0 0.0 INIAP 414 48.3 0.3 0.1 0.0 0.0 0.0 0.0 0.2 PI321094-D 47.3 0.8 0.3 0.0 0.0 0.0 0.8 0.2 USDK-4 44.0 0.2 0.1 0.1 0.0 0.0 0.1 0.3 NE34-12-28 47.7 1.3 0.3 0.0 0.0 0.0 0.0 0.0 NE34-12-50 46.3 0.4 0.3 0.0 0.0 0.0 0.0 0.2 LOCAL 1 44.8 0.7 0.2 0.1 0.0 0.0 0.0 0.1 LOCAL 2 48.2 0.2 0.0 0.0 0.0 0.3 0.1 0.5 LOCAL 3 49.6 0.7 0.3 0.0 0.0 0.0 0.0 0.2 LOCAL 4 46.9 0.4 0.1 0.0 0.0 0.0 0.0 0.5

Misamfu, Zambia 2015

Entry rootr als cbb ant rust bcmv yielp MBULAMTWE 1.1 6.0 2.7 1.8 2.2 1.3 175.6 Incomparable 1.1 6.2 2.7 1.3 3.9 1.2 143.5 G 10994 1.1 4.8 2.1 1.1 6.8 1.6 12.7 Larga Comercial 1.1 3.5 2.3 1.3 1.4 1.3 164.2 Carioca, Kihala 1.2 2.7 2.5 1.1 1.2 1.4 197.7 USDK-4 1.0 5.4 2.5 1.3 2.5 1.2 178.1 Clouseau 1.0 5.8 2.6 1.3 3.0 1.2 80.3 NE34-12-28 1.0 3.9 3.2 1.3 2.4 1.6 252.2 NE34-12-34 1.0 4.8 3.3 1.8 3.9 1.8 231.7 NE34-12-45 1.2 3.3 2.8 1.4 4.1 1.8 150.9 NE34-12-47 1.1 3.8 2.3 1.1 1.4 1.3 87.0 NE34-12-50 1.1 1.9 1.7 1.0 4.8 1.2 152.7 Local 1 D 1.1 3.5 2.3 1.2 1.9 1.6 179.8 Local 2 D 1.1 2.4 2.7 1.7 1.4 2.0 178.2 Local 1 I 1.1 4.2 2.3 1.2 3.5 1.2 158.8 Local 2 I 1.3 3.7 2.2 1.3 1.4 1.9 148.3

Future Plans

Elite adapted East Africa/Southern Africa lines will be crossed to several sources of germplasm possessing multiple disease resistance and good agronomic traits,

Future Plans

Once the protocols for various root pathogens are established, we will develop different RILs to identify QTLs regions associated with root rot resistance. Some of the RILs showing resistance to root rot pathogens will be released as either germplasm or cultivars.