If you have a Cell Project on the front counter, please take it now (put on hallway table and

take it after class).Materials: ISN

1. Define extraction in your own words. 2. How do you suppose we will extract DNA

from strawberries and peas?3. What are your predictions for how DNA

will look?

DNA ExtractionDNA is the genetic code-of lifeDNA is found in the chromosomesChromosomes are found in the nucleusAll eukaryotic cells have a nucleusAll living organisms are made of cells

DNA Extraction - StepsCollect cellsBurst cell membranes open - saltDissolve lipid (fat) layer - soapSeparate DNA from proteins and debris - saltIsolate concentrated DNA – 91% Isopropyl

Alcohol (stored in freezer)

Take the green part off the strawberry

Add the strawberry, 10 ml. water, and 3 pinches of salt to the baggy.

“Smoosh” the strawberry with your fingers

Use the stick to get a drop of soap

Add a drop of soap to the baggy

Gently rock the baggy back-and-forth (so you don’t make bubbles)

Put the coffee filter into the funnel

Slowly pour the strawberry juice into the filter

It takes time to filter through

Pour the juice into the smaller beaker or test tube

Tilt both containers when you pour the alcohol down the side

Pull the DNA out with the pipette

Drawing Conclusions1. Why was it necessary to add salt to the

mixture?2. Why was it necessary to add soap to the

mixture?3. What did the DNA look like? 4. Explain how DNA is stored in the nucleus.5. Explain what happened in the final step

when you added the alcohol.6. List two reasons why it is important for

scientists to remove DNA from an organism.