iit- gandhi nagar 2016
TRANSCRIPT
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MAKING TECHNOLOGY FROM NATURE- SOME FEASIBLE TRENDS
Dr. C. Mohankumar
SCMS Institute of Bioscience and Biotechnology Research & Development (SIBBR&D)
Recognized under Scientific and Industrial Research Organization (SIRO), DSIR, Government of India.
Approved Research center of Anna University, Chennai
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“ It is science alone that can solve the problems of hunger and poverty, of
insanitation and illiteracy, of superstition and deadening of custom and tradition, of vast resources running to waste, or a
rich country inhabited by starving poor…..
“ The future belongs to science and those who make friends with science ”
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21st century is for Biotechnology Industry
The power of scientific research becomes a reality only through the development of viable technologies i.e. Industry- friendly technology.
The trend of developing cost effective technology for the production nutraceuticals, antibiotics , therapeutics like anti-diabetic and anticancer drugs, ecofriendly Biofuel, cosmetics and other value added products has generated a novel research culture in biotech industry.
Today the fascination of scientists and researchers towards biotechnology research has increased due to the application of biological concepts using appropriate tools and techniques.
Based on the conceptual advancement, biotech products unlike natural and synthetic always maintain a sustainable quality. So biotechnologists strive to recreate natural processes using Omic science.
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Omics science is a new generation concept that connects all the biological, biochemical & life sciences to a common platform of research.
It is the collective study of three biochemical molecules (DNA- RNA- Proteins) that transcript, translate and function at different phases of each metabolic event in the cell system of an organism.
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Milk protein Technology
Cattle colloquially termed as cows is an important part of our socio-agricultural system in India. Cows occupy 38% of the entire livestock population in the country
As per the list of National Bureau of Animal Genetics Resources (NBAGR), India has 34 indigenous recognized breeds with independent accession numbers
Bos indicus -Vechur cow is an indigenous domestic breed of Kerala with accession numbers Indian cattle 0900/Vechur cow 03030. Categorized as Critical breed as FAO
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Smallest cow in the world
High
adaptability
Medicinal
property of Milk
Efficient
fodder
conversion
to milk
Easy
Domestic
ation
Better yield & disease
resistance
Vechur Breed
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Water 87.7 %
Proteins 3.3 %
Minor Constituents
0.7 %Carbohydrates (Lactose) 4.9 %
Fats 3.4 %
MILK
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Milk Proteins
Insoluble Protein (80%) Soluble Protein (20%)Whey Protein
CaseinLactoferrinLactoperoxidaseLactalbuminLactoglobulin Lactoglobulin
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Multidimensional properties of Lactoferrin
Antibacterial
Immunomodulation
Anticancer
Iron Absorption
Antifungal
Antiviral
AntiparasiticAnti-inflammatory
Protease inhibitor
Bone formation
Lf
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Exon
Intron
Lactoferrin gene – 25-35 Kb
Coding region – 2.1 Kb distributed in 17 exons
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10 Kb
4.0
2.5
2.0
1.5
1.0
PCR Amplification of Bovine Lactoferrin gene
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Nucleotide and Amino acid sequence of
Bovine Lactoferrin Gene accession no.
JF926526, NCBI
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Amino acid Variation BLf Vechur Position
Alanine A/T Threonine 7
Alanine A/V Valine 61
Isoleucine I/V Valine 145
Cysteine C/R Arginine 176, 264
Proline P/S Serine 428
Serine S/T Threonine 538
Treonine T/N Asparagine 546
Lysine K/E Glutamic acid 627
Histidine H/R Arginine 632
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Bacterial Expression of Recombinant Bovine
Lactoferrin protein
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Antibacterial Property of Recombinant Bovine Lactoferrin protein
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Fruit protein Technology.
Pineapple Protein-Bromelain.
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Bromelain
DIGESTION
ANTICOAGULANT
ANTI-BACTERIAL
WOUND HEALING
ANTI-INFLAMMATORY
ANTI- CANCER
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PCR amplified BRM Gene
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Vector construct for eukaryotic expression of BRM gene
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Callus cultureSuspension culture
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Amplified BRM gene from cell suspension culture of N.tabacum
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Western Blot analysis of rBRM protein from eukaryotic expression
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A) Evenly spread fungal mycelium in control plateB) Inhibition of mycelium spread in rBRM treated plates
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Antibacterial sensitivity test
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Native Bromelain
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Effect of native BRM, recombinant BRM and anticancer drugs Oncodox, Fluracil and Cycloxan in the inhibition of cell
multiplication of human breast cancer cell line - MCF-7 cells
Oncodox Fluracil Cycloxan rBRM -10 ug rBRM – 50 ug nBRM- 10 ug nBRM- 50 ug
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COCONUT BIOFUEL TECHNOLOGY
An Ecofriendly Biofuel of Third World
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Properties Test values
Moisture Content 0.05%
Free fatty Acid (mg KOH/g) 0.1%
Density (g/cm3) 0.920
Saponification Value 249.4
Iodine Value 7.7
Peroxide Value Nil
Refractive index 1.45
Non saponifiable matter 0.05-0.1 %
Physicochemical properties of coconut oil
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Preheated Coconut Oil(850 L)
Addition of Sodium Methoxide and Transesterification
Layer separation
Glycerol Layer Coconut Methyl Ester
CME as Biofuel (765 L)
Crude Glycerol (85 L)
Washing & Dehydration
Processing profile of coconut oil to CME and glycerol by Transesterification
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Qua
ntity
of C
ME
& G
lyce
rol (
ml)
Transesterification of coconut oil
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Sl No: Test Test values Standard Biodiesel1 Acidity (inorganic) Nil 0-0.5
2 Acidity (Total) 0.24 mg/KOH/g 0.2-0.5 mg/KOH/g
3 Ash Nil 0
4 Carbon residue (Ramsbottom)on 10% residue <0.1 0.3
5 Cetane Number 42.1 41-566 Cetane index 40.20 -7 Cold filter plugging point <-9 -4-+3 °C
8 Copper strip corrosion, 3Hrs @100°C 1b 1b
9 Density @ 15°C 884.3 kg/m3 860 -900 Kg/m3
10 Flash point (Abel/PMC) 96°C 93-120°C11 Kinematic Viscosity @ 40°C 3.65 cST 3.5-5.0 cST
12 Lubricity, corrected wear scar diameter (WSD) @ 60°C 151 Microns 150-155 Microns
13 Oxidation Stability 14 g/m3 12-16 g/m3
14 Pour Point -3 °C -3 – 0 °C15 Total contamination 13 mg/Kg 24 mg/Kg16 Total sulphur Nil 0.001 – 0.05%17 Water Content 500mg/Kg 500 mg/ Kg
Physicochemical properties of Coconut methyl ester against biodiesel standard
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Physicochemical properties of CME with other methyl esters of plant origin
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Cetane number (CN) – the primary indicator of ignition quality of fuel.Optimal range of CN is between 41 -56 based on global standards CN depends on chemical structure of vegetable oil, degree of unsaturation, number of carbon atoms and the nature of alcohol used for esterification of the oil
Density of Biofuel @ 15°CAn effective parameter of fuel qualityDensity affects fuel performance, quality of atomization & combustion.
Viscosity of biofuelCrucial factor of liquid fuels. It influence the ease starting of engine, spray quality, size of the particle. penetration of the injected jet and quality of fuel-air mixture combustion.
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Pour point (PP) -3°C and cold filter plugging point (CFPP) -9°C represents the fuel efficiency in cold environment
Flash point of CME 96°C indicates ability of ignition Makes the fuel comfortable during storage, transportation and handling
Lubricity, oxidation stability, water content and acid value supports the competent nature of CME as good biofuel
Low carbon residue (0.1), minimal acidity, absence of sulphur element elevate the quality of biofuel as ecofriendly.
The properties of CME such as viscosity, acid value flash point, pour point and cetane number were found different and unique compared to other plant methyl esters (PME)
High percentage of short chain fatty acids, lipid saturation and the other physicochemical properties of coconut oil are instrumental in inducing the quality of CME as a perfect biofuel.
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Engine Type 4 Stroke, Naturally aspirated, Indirect injection, Water cooled diesel engine.
Engine Capacity (CC) 702
Emission Norms BSIII and BSIV
Power (HP at rpm) 16 at 3200 rpm
Torque (Nm at rpm) 38 at 2500 rpm
Technical specifications of the new diesel vehicle - TATA Ace-Magic
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Efficacy of CME in Diesel Engine
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Technical advantages of CME
Can be used straight away as fuel in diesel enginesHigher mileage than fossil fuelNo modification required in engine componentsNo modification required in fuel linesNeed not be blended with any other fuel in diesel enginesNo additives required
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1
2
3
4
shell
Enriched MCW
Coconut cake
Glycerol
CME
Dehusked coconuts
Dried endosperm
Coconut oil
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Material balance of 10,000 dehusked nuts processed for the production of coconut oil. The quantity and the market value of each product is given in bracket.
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Schematic diagram showing the protocol of processing mature coconut water (MCW) derived as sub product during the processing of dehusked nuts.
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HIGHLIGHTS OF COCONUT BIOFUEL
1. Rapid and easy processing.2. Effective Utilization of sub products makes it cost effective.3. Physico-chemical features of CME are competent as a good
fuel.4. Low carbon residue, absence of Sulphur elements and other
contaminants support the ecofriendly nature
5. A new horizon for coconut growers and farmers.
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