immune cell isolation & culture · 2019. 5. 16. · portfolio provides both an unparalleled...
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Immune Cell Isolation & Culture Immune cell isolation and culture are necessary for both basic research aimed at investigating the functions of different immune cell types and for expanding specific immune cell populations for therapeutic purposes. Bio-Techne is focused on providing the highest quality reagents for isolating, activating, differentiating, and culturing immune cells. From natural killer (NK) cells and macrophages to dendritic cells and T cells, we offer kits for both cell enrichment and cell differentiation or expansion, along with cell culture media, fetal bovine serum, and an unparalleled selection of recombinant bioactive proteins and antibodies. In addition to our reagents for basic research, we continue to expand our selection of innovative tools for ex vivo cell manufacturing, making it easier for researchers to transition their research from discovery to the clinic. Our portfolio now includes GMP-grade recombinant proteins and GMP-manufactured custom cell separation and CD3/CD28 T cell activation kits, which both utilize non-magnetic Cloudz™ dissolvable microparticle technology.
Learn more | rndsystems.com/immunecellculture
ContentsIntroduction to Immune Cell Isolation & Culture ......................................................................................................................................2
MagCellect™ Cell Selection Kits & Reagents .............................................................................................................................................3
T Cell Enrichment Columns ........................................................................................................................................................................4
CellXVivo™ Kits for Immune Cell Differentiation or Expansion .................................................................................................................4
Cell Culture Media .......................................................................................................................................................................................5
Classic Fetal Bovine Serum ........................................................................................................................................................................6
Recombinant Cytokines for Immune Cell Differentiation and Expansion ..............................................................................................7
ProDots® Proteins ..................................................................................................................................................................................... 13
Animal-Free™ and Animal Component-Free Process (ACFP) Recombinant Proteins ............................................................................................................................................................................. 13
GMP-grade Immune Cell Culture Reagents ............................................................................................................................................ 14
Cloudz™ Cell Selection Kits ...................................................................................................................................................................... 14
GMP-grade Recombinant Proteins .......................................................................................................................................................... 15
GMP Cloudz™ T Cell Activation Kit – CD3/CD28 .................................................................................................................................... 15
Custom Services ...................................................................................................................................................................................... 15
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Learn more | rndsystems.com/magcellect
MagCellect™ Cell Selection Kits & ReagentsIsolation or enrichment of a specific cell type of interest from a heterogeneous cell population is frequently a critical first step in immunology research for obtaining a pure population of cells for further characterization. R&D Systems® MagCellect Cell Selection Kits are designed to enrich for specific cell populations based on either a negative or posi-tive selection principle. Features of the kits include:
• Beads with a high binding capacity
• Small beads in ferrofluid that have no magnetic memory and will not induce cell damage
• Cells can be separated to a very high purity in minutes
Isolation of Human CD19+ B Cells from PBMCs using the MagCellect Human B Cell Isolation Kit. Human PBMCs were prepared as described in the cell preparation section of the MagCellect Human B Cell Isolation Kit (R&D Systems, Catalog # MAGH103). Cells were stained both before (A) and after (B) B cell isolation with the MagCellect Kit using a PE-conjugated Mouse Anti-Human CD19 Monoclonal Antibody (R&D Systems, Catalog # FAB4867P) and an APC-conjugated Mouse Anti-Human CD14 Monoclonal Antibody (R&D Systems, Catalog # FAB3832A).
-103
103
104
105
-103 104103 105
CD14
A B
0
0CD19
-103
103
104
105
-103 104103 105
CD14
0
0CD19
-103
103
104
105
-103 104103 105
CD14
A B
0
0CD19
-103
103
104
105
-103 104103 105
CD14
0
0CD19
MagCellect Human Cell Isolation Kits Catalog #
Human B Cell Isolation Kit MAGH103
Human CD14+ Cell Isolation Kit MAGH105
Human CD3+ T Cell Isolation Kit MAGH101
Human Naïve CD4+ T Cell Isolation Kit MAGH115
Human CD4+ T Cell Isolation Kit MAGH102
Human Memory CD4+ T Cell Isolation Kit MAGH116
Human CD4+CD25+ Regulatory T Cell Isolation Kit MAGH104
Human CD8+ T Cell Isolation Kit MAGH112
Human Natural Killer Cell Isolation Kit MAGH109
MagCellect Mouse Cell Isolation Kits Catalog #
Mouse B Cell Isolation Kit MAGM204
Mouse CD3+ T Cell Isolation Kit MAGM201
Mouse Naïve CD4+ T Cell Isolation Kit MAGM205
Mouse CD4+ T Cell Isolation Kit MAGM202
Mouse Memory CD4+ T Cell Isolation Kit MAGM206
Mouse Naïve CD8+ T Cell Isolation Kit MAGM207
Mouse CD4+CD25+ Regulatory T Cell Isolation Kit MAGM208
Mouse CD8+ T Cell Isolation Kit MAGM203
Mouse Natural Killer Cell Isolation Kit MAGM210
MagCellect Rat Cell Isolation Kits Catalog #
Rat B Cell Isolation Kit MAGR303
Rat CD3+ T Cell Isolation Kit MAGR301B
Rat CD4+ T Cell Isolation Kit MAGR302B
MagCellect Plus Human Kits Catalog #
Human CD45+ Cell Isolation Kit MAGH125
MagCellect Reagents Catalog #
MagCellect Magnet MAG997
MagCellect Streptavidin Ferrofluid MAG999B
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T Cell Enrichment ColumnsIn addition to our MagCellect Kits, Bio-Techne also offers human and mouse T cell enrichment columns from our R&D Systems brand that are designed to enrich for a specific T cell population from a mononuclear cell suspension.
CellXVivo™ Kits for Immune Cell Differentiation or ExpansionR&D Systems CellXVivo Immune Cell Differentiation or Expansion Kits contain optimized combinations of the highest quality proteins and antibodies, along with simple, reproducible protocols for the differentiation or expansion of den-dritic cells, macrophages, T helper cell subtypes, regulatory T cells, B cells, natural killer cells, or cytokine-induced killer (CIK) cells. Benefits of the kits include:• Conveniently packages all reagents necessary for immune cell differentiation or expansion
• Guaranteed to produce consistent, reliable results
• Cost-effective and efficient
Human T Cell Enrichment Columns Catalog #
Human CD3+ T Cell Enrichment Column HTCC
Human CD4+ T Cell Enrichment Column HCD4C-1000
Human CD4+ T Cell Enrichment Column, Mini HCD43
Human CD4+/CD45RA- Memory T Cell Enrichment Column HCD42
Human CD4+/CD45RO- Naïve T Cell Enrichment Column HCD41
Human CD8+ T Cell Enrichment Column HCD8C-1000
Human CD8+/CD45RA- Memory T Cell Enrichment Column HCD45
Human CD8+/CD45RO- Naïve T Cell Enrichment Column HCD44
Mouse T Cell Enrichment Columns Catalog #
Mouse CD3+ T Cell Enrichment Column MTCC
Mouse CD4+ T Cell Enrichment Column MCD4C-1000
Mouse CD4+ T Cell Enrichment Column, Mini MCD43
Mouse CD8+ T Cell Enrichment Column MCD8C-1000
Human CellXVivo Differentiation or Expansion Kits Catalog #
Human B Cell Expansion Kit CDK005
Human CIK Expansion Kit CDK014
Human M1 Macrophage Differentiation Kit CDK012
Human M2 Macrophage Differentiation Kit CDK013
Human Monocyte-derived DC Differentiation Kit CDK004
Human NK Cell Expansion Kit CDK015
Human Th1 Cell Differentiation Kit CDK001
Human Th2 Cell Differentiation Kit CDK002
Human Th17 Cell Differentiation Kit CDK003C
Human Treg Cell Differentiation Kit CDK006
Mouse CellXVivo Differentiation Kits Catalog #
Mouse Dendritic Cell Differentiation Kit CDK008
Mouse Th1 Cell Differentiation Kit CDK018
Mouse Th2 Cell Differentiation Kit CDK019
Mouse Th17 Cell Differentiation Kit CDK017
Mouse Treg Cell Differentiation Kit CDK007
Intracellular Cytokine Staining of Mouse Th17 Cells Following Differentiation using the CellXVivo Mouse Th17 Cell Differentiation Kit. Mouse naïve CD4+ T cells without (A, C) or with (B, D) a 5-day differentiation using the reagents provided in the CellXVivo Mouse Th17 Differentiation Kit (R&D Systems, Catalog # CDK017) were stimulated with Cell Activation Cocktail (Tocris, Catalog # 5476) and stained with a PE-conjugated Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-72027) and either an (A, B) APC-conjugated Rabbit Anti-Mouse IL-4 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-52656) or an (C, D) APC-conjugated Rat Anti-Mouse IFN-g Monoclonal Antibody (R&D Systems, Catalog # IC485A) and detected by flow cytometry. Quadrants were set based on staining with the appropriate isotype controls.
IL-1
7
100
101
100
102
103
104
101 102 103 104
IL-4
0.1%
0.5%
0.9%
98.5%
A
IL-1
7
100
101
100
102
103
104
101 102 103 104
IL-4
0.1%
0.1%50.6%
49.1%B
IL-1
7
100
101
100
102
103
104
101 102 103 104
IFN-γ
0.1%
0.1%
0.5%
99.4%
C D
IL-1
7
100
101
100
102
103
104
101 102 103 104
IFN-γ
0.1%
0.3%
47.6%
51.9%
Learn more | rndsystems.com/cellxvivo
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Cell Culture Media
Bio-Techne now offers Atlanta Biologicals™ cell culture media including both RPMI 1640 and DMEM, as well as cell culture serum, media supplements, and cell culture antibiotics. With over 40 years of research and development experience invested, Atlanta Biologicals cell culture portfolio provides both an unparalleled commitment to quality and exceptional product value.
Cell Culture Media Catalog #
RPMI 1640, 25mM HEPES, no glutamine M30050
RPMI 1640, L-glutamine M30150
RPMI 1640, 25mM HEPES, L-glutamine M30250
RPMI 1640, L-glutamine, no phenol red M30350
RPMI 1640, no glutamine M30450
RPMI 1640, 25mM HEPES, no glutamine, no phenol red M30550
RPMI 1640, no glutamine, no phenol red M30650
RPMI 1640, 25mM HEPES, L-glutamine, no phenol red M30750
RPMI 1640, GlutaminePlus M30850
RPMI 1640, 25mM HEPES, GlutaminePlus M31050
RPMI 1640, 25mM HEPES, GlutaminePlus, no phenol red M31350
RPMI 1640, GlutaminePlus, no phenol red M31550
DMEM, low glucose, no glutamine, sodium pyruvate M12150
DMEM, low glucose, no glutamine, no sodium pyruvate, no phenol red M12250
DMEM, low glucose, 25mM HEPES, no glutamine, sodium pyruvate M12350
DMEM, high glucose, no glutamine, sodium pyruvate M12450
DMEM, low glucose, 25mM HEPES, no glutamine, sodium pyruvate, no phenol red M12550
DMEM, low glucose, no glutamine, sodium pyruvate, no phenol red M12650
DMEM, low glucose 25mM HEPES, glutamine, sodium pyruvate, no phenol red M12750
DMEM, low glucose, glutamine, sodium pyruvate, no phenol red M12850
DMEM, high glucose, L-glutamine, no sodium pyruvate, no phenol red M12950
DMEM, low glucose, 25mM HEPES, GlutaminePlus, sodium pyruvate M13050
DMEM, low glucose, 25mM HEPES, GlutaminePlus, sodium pyruvate, no phenol red M13150
DMEM, low glucose, GlutaminePlus, sodium pyruvate M13250
DMEM, low glucose, GlutaminePlus, sodium pyruvate, no phenol red M13350
DMEM, low glucose, 25mM HEPES, no glutamine, no sodium pyruvate M13850
DMEM, low glucose, 25mM HEPES, no glutamine, no sodium pyruvate, no phenol red M13950
DMEM, low glucose, no glutamine, no sodium pyruvate M14050
DMEM, low glucose, 25mM HEPES, glutamine, no sodium pyruvate M17050
DMEM, low glucose, 25mM HEPES, glutamine, no sodium pyruvate, no phenol red M17150
DMEM, low glucose, glutamine, no sodium pyruvate M17250
DMEM, low glucose, glutamine, no sodium pyruvate, no phenol red M17350
DMEM, low glucose, 25mM HEPES, GlutaminePlus, no sodium pyruvate M17450
DMEM, low glucose, 25mM HEPES, GlutaminePlus, no sodium pyruvate, no phenol red M17550
Cell Culture Media Catalog #
DMEM, low glucose, GlutaminePlus, no sodium pyruvate M17650
DMEM, low glucose, GlutaminePlus, no sodium pyruvate, no phenol red M17750
DMEM, high glucose, 25mM HEPES, no glutamine, sodium pyruvate M18250
DMEM, high glucose, 25mM HEPES, no glutamine, sodium pyruvate, no phenol red M18350
DMEM, high glucose, no glutamine, sodium pyruvate, no phenol red M18450
DMEM, high glucose, 25mM HEPES, L-glutamine, sodium pyruvate, no phenol red M18550
DMEM, high glucose, L-glutamine, sodium pyruvate, no phenol red M18650
DMEM, high glucose, 25mM HEPES, GlutaminePlus, sodium pyruvate M18750
DMEM, high glucose, 25mM HEPES, GlutaminePlus, sodium pyruvate, no phenol red M18850
DMEM, high glucose, GlutaminePlus, sodium pyruvate M18950
DMEM, high glucose, GlutaminePlus, sodium pyruvate, no phenol red M19050
DMEM, high glucose, 25mM HEPES, no glutamine, no sodium pyruvate M19550
DMEM, high glucose, 25mM HEPES, no glutamine, no sodium pyruvate, no phenol red M19650
DMEM, high glucose, 25mM HEPES, L-glutamine, no sodium pyruvate, no phenol red M19750
DMEM, high glucose, 25mM HEPES, GlutaminePlus, no sodium pyruvate M19850
DMEM, high glucose, 25mM HEPES, GlutaminePlus, no sodium pyruvate, no phenol red M19950
DMEM, low glucose, glutamine, sodium pyruvate M22150
DMEM, high glucose, L-glutamine, no sodium pyruvate M22250
DMEM, low glucose, 25mM HEPES, glutamine, sodium pyruvate M22350
DMEM, high glucose, L-glutamine, sodium pyruvate M22450
DMEM, high glucose, 25mM HEPES, L-glutamine, no sodium pyruvate M22550
DMEM, high glucose, 25mM HEPES, L-glutamine, sodium pyruvate M22650
DMEM, high glucose, no glutamine, no sodium pyruvate M22850
DMEM, high glucose, no glutamine, no sodium pyruvate, no phenol red M22950
DMEM, high glucose, GlutaminePlus, no sodium pyruvate, no phenol red M32350
DMEM, high glucose, GlutaminePlus, no sodium pyruvate M32650
Learn more | atlanta-biologicals.com
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Classic Fetal Bovine Serum
Fetal bovine serum (FBS) is the ideal growth supplement for cell culture media and is generally preferred over other types of sera due to its high levels of nutrients and optimal combination of growth factors. Atlanta Biologicals FBS is produced utilizing strict manufacturing processes and quality assurance procedures, which guarantees unsurpassed quality and consistency from lot-to-lot.
Reagents and SupplementsIn addition to media and serum, Bio-Techne also offers Atlanta Biologicals media supplements, balanced salt solutions, biological buffers, cell freezing media, and more.
Fetal Bovine Serum
Product Size Regular (Catalog #) Heat-inactivated (Catalog #)
Fetal Bovine Serum - Optima
50 mL S12495 S12495H
100 mL S12410 S12410H
500 mL S12450 S12450H
Fetal Bovine Serum - Premium
50 mL S11195 S11195H
100 mL S11110 S11110H
500 mL S11150 S11150H
Fetal Bovine Serum - Premium Select
50 mL S11595 S11595H
100 mL S11510 S11510H
500 mL S11550 S11550H
Other Bovine Serum
Product Size Regular (Catalog #) Heat-inactivated (Catalog #)
Newborn Calf Serum
50 mL S11295 S11295H
100 mL S11210 S11210H
500 mL S11250 S11250H
Calf Serum
50 mL S11495 S11495H
100 mL S11410 S11410H
500 mL S11450 S11450H
Iron-Supplemented Calf Serum
50 mL S11995 S11995H
100 mL S11910 S11910H
500 mL S11950 S11950H
Donor Bovine Serum
50 mL S11395 S11395H
100 mL S11310 S11310H
500 mL S11350 S11350H
Learn more | atlanta-biologicals.com
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Recombinant Cytokines for Immune Cell Differentiation and Expansion
R&D Systems recombinant and natural proteins are the most referenced proteins in the scientific literature because researchers trust our strong reputation for product quality. We have over 25 years of experience expressing and purifying proteins using a variety of different systems, maintain stringent purification and production standards, and are committed to rigorously testing the bioactivity of our proteins prior to their release to ensure that they will provide researchers with industry-leading bioactivity and lot-to-lot consistency. The section below shows the recombinant proteins and antibodies (when applicable) that are most frequently used for the activation, differentiation, and expansion of different immune cell types, along with specialized serum-free media that has been developed for the culture of human dendritic cells and T lymphocytes. In addition to the standard recombinant proteins shown in the product tables below, our portfolio also includes R&D Systems Animal-Free™ and Animal Component-Free Process recombinant proteins, ProDots® proteins, GMP-grade recombinant proteins, and custom protein development services, which are detailed in the sections that follow.
B Cells
Molecule Species Source Catalog #
BAFF/Blys/TNFSF13B
Human CHO 7537-BF
Human NS0 2149-BF
Mouse NS0 8876-BF
CD40 Ligand/TNFSF5
Human HEK293 6420-CL
Human E. coli 2706-CL
Human E. coli 6245-CL
Mouse CHO 8230-CL
Rat CHO 8414-CL
IFN-g
Human E. coli 285-IF
Human HEK293 10067-IF
Mouse E. coli 485-MI
Rat E. coli 585-IF
IL-2
Human E. coli 202-IL
Mouse E. coli 402-ML
Rat E. coli 502-RL
IL-4
Human E. coli 204-IL
Human CHO 6507-IL
Mouse E. coli 404-ML
Rat E. coli 504-RL
IL-10
Human Sf21 (baculovirus) 217-IL
Human Sf21 (stably transfected) 217-ILB
Human E. coli 1064-IL
Mouse E. coli 417-ML
Rat E. coli 522-RLB
IL-21Human E. coli 8879-IL
Mouse E. coli 594-ML
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Dendritic Cells
Molecule Species Source Catalog #
Flt-3 Ligand
Human Sf21 (baculovirus) 308-FK
Human NS0 308-FKN
Mouse NS0 427-FL
GM-CSF
Human E. coli 215-GM
Human CHO 7954-GM
Mouse E. coli 415-ML
Rat E. coli 518-GM
IL-3
Human E. coli 203-IL
Mouse E. coli 403-ML
Rat E. coli 2524-RL
IL-4
Human E. coli 204-IL
Human CHO 6507-IL
Mouse E. coli 404-ML
Rat E. coli 504-RL
Thrombopoietin
Human E. coli 288-TPE
Human NS0 288-TPN
Human Sf21 (baculovirus) 288-TP
Mouse NS0 488-TO
Rat NS0 7864-TP
TNF-a
Human E. coli 210-TA
Mouse E. coli 410-MT
Mouse E. coli 410-TRNC
Rat E. coli 510-RT
Macrophages
Molecule Species Source Catalog #
M-CSF
Human E. coli 216-MC
Human CHO 216-MCC
Mouse E. coli 416-ML
IFN-g
Human E. coli 285-IF
Human HEK293 10067-IF
Mouse E. coli 485-MI
Rat E. coli 585-IF
IL-4
Human E. coli 204-IL
Human CHO 6507-IL
Mouse E. coli 404-ML
Rat E. coli 504-RL
IL-13
Human E. coli 213-ILB
Mouse E. coli 413-ML
Rat E. coli 1945-RL
Specialized Media
Product Catalog #
StemXVivo® Serum-Free Human Dendritic Cell Base Media CCM003
dendritic cell
Antibody Application KeyB/N Blocking/Neutralization Depl Cell Depletion Ecap ELISA Capture FA Functional Assay FC Flow Cytometry ICC/IF Immunocytochemistry/Immunofluorescence IHC Immunohistochemistry WB Western Blot
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Myeloid-derived Suppressor Cells (MDSCs)
Molecule Species Source Catalog #
G-CSFHuman E. coli 214-CS
Mouse E. coli 414-CS
GM-CSF
Human E. coli 215-GM
Human CHO 7954-GM
Mouse E. coli 415-ML
Rat E. coli 518-GM
IL-4
Human E. coli 204-IL
Human CHO 6507-IL
Mouse E. coli 404-ML
Rat E. coli 504-RL
IL-6
Human E. coli 206-IL
Human HEK293 7270-IL
Mouse E. coli 406-ML
Rat E. coli 506-RL
IL-10
Human Sf21 (baculovirus) 217-IL
Human Sf21 (stably transfected) 217-ILB
Human E. coli 1064-IL
Mouse E. coli 417-ML
IL-13
Human E. coli 213-ILB
Mouse E. coli 413-ML
Rat E. coli 1945-RL
Natural Killer (NK) Cells
Molecule Species Source Catalog #
IL-2
Human E. coli 202-IL
Mouse E. coli 402-ML
Rat E. coli 502-RL
IL-12
Human Sf21 (baculovirus) 219-IL
Human HEK293 10018-IL
Mouse Sf21 (baculovirus) 419-ML
Mouse NS0 10051-ML
Rat Sf21 (baculovirus) 1760-RL
IL-15Human E. coli 247-ILB
Mouse E. coli 447-ML
IL-18/IL-1F4
Human E. coli 9124-IL
Human E. coli B001-5
Mouse E. coli 9139-IL
Mouse E. coli B002-5
IL-21Human E. coli 8879-IL
Mouse E. coli 594-ML
Cytokine-induced Killer (CIK) Cells
Molecule Species Source Catalog #
IL-2
Human E. coli 202-IL
Mouse E. coli 402-ML
Rat E. coli 502-RL
IFN-g
Human E. coli 285-IF
Human HEK293 10067-IF
Mouse E. coli 485-MI
Rat E. coli 585-IF
Antibodies Species Clone Catalog # (Applications)
CD3Human UCHT1 MAB100 (FA, FC, ICC/IF, IP)
Mouse 145-2C11 MAB484 (Depl., FA, FC, IP)
cytokine induced killer
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Regulatory T Cells
Molecule Species Source Catalog #
IL-2
Human E. coli 202-IL
Mouse E. coli 402-ML
Rat E. coli 502-RL
TGF-b1
Human HEK293 7754-BH
Human CHO 240-B
Human Platelets 100-B
Mouse CHO 7666-MB
Antibodies Species Clone Catalog # (Applications)
CD3Human UCHT1 MAB100 (FA, FC, ICC/IF, IP)
Mouse 145-2C11 MAB484 (Depl., FA, FC, IP)
CD28
Human 37407 MAB342 (FA, WB)
Human Polyclonal AF-342-PB (FA, FC, ICC/IF, IHC)
Mouse 794716 MAB4832 (FA, FC)
T Cells
Molecule Species Source Catalog #
IL-2
Human E. coli 202-IL
Mouse E. coli 402-ML
Rat E. coli 502-RL
Antibodies Species Clone Catalog # (Applications)
CD3Human UCHT1 MAB100 (FA, FC, ICC/IF, IP)
Mouse 145-2C11 MAB484 (Depl., FA, FC, IP)
CD28
Human 37407 MAB342 (FA, WB)
Human Polyclonal AF-342-PB (FA, FC, ICC/IF, IHC)
Mouse 794716 MAB4832 (FA, FC)
Specialized Media
Product Catalog #
StemXVivo® Serum-Free Human T Cell Base Media CCM010
regulatory t cell
Antibody Application KeyB/N Blocking/Neutralization Depl Cell Depletion Ecap ELISA Capture FA Functional Assay FC Flow Cytometry ICC/IF Immunocytochemistry/Immunofluorescence IHC Immunohistochemistry WB Western Blot
11
Th1 Cells
Molecule Species Source Catalog #
IFN-g
Human E. coli 285-IF
Human HEK293 10067-IF
Mouse E. coli 485-MI
Rat E. coli 585-IF
IL-2
Human E. coli 202-IL
Mouse E. coli 402-ML
Rat E. coli 502-RL
IL-12
Human Sf21 (baculovirus) 219-IL
Human HEK293 10018-IL
Mouse Sf21 (baculovirus) 419-ML
Mouse NS0 10051-ML
Rat Sf21 (baculovirus) 1760-RL
IL-27Human NS0 2526-IL
Mouse NS0 2799-ML
Antibodies Species Clone Catalog # (Applications)
CD3Human UCHT1 MAB100 (FA, FC, ICC/IF, IP)
Mouse 145-2C11 MAB484 (Depl., FA, FC, IP)
CD28
Human 37407 MAB342 (FA, WB)
Human Polyclonal AF-342-PB (FA, FC, ICC/IF, IHC)
Mouse 794716 MAB4832 (FA, FC)
IL-4
Human 34019 MAB204 (B/N, WB)
Human 3007 MAB304 (B/N, ICC/IF, WB)
Human Polyclonal AF-204-NA (B/N, WB)
Mouse 30340 MAB404 (B/N, Ecap, WB)
Mouse Polyclonal AF-404-NA (B/N, WB)
Mouse 30340R MAB404R (B/N, Ecap)
Th2 CellsMolecule Species Source Catalog #
IL-2
Human E. coli 202-IL
Mouse E. coli 402-ML
Rat E. coli 502-RL
IL-4
Human E. coli 204-IL
Human CHO 6507-IL
Mouse E. coli 404-ML
Rat E. coli 504-RL
Antibodies Species Clone Catalog # (Applications)
CD3Human UCHT1 MAB100 (FA, FC, ICC/IF, IP)
Mouse 145-2C11 MAB484 (Depl., FA, FC, IP)
CD28
Human 37407 MAB342 (FA, WB)
Human Polyclonal AF-342-PB (FA, FC, ICC/IF, IHC)
Mouse 794716 MAB4832 (FA, FC)
IFN-g
Human 25718 MAB285 (B/N, FC, ICC/IF)
Human 25718R MAB285R (B/N, ICC/IF)
Human K3.53 MAB2852 (B/N, Ecap, FC, WB)
Human Polyclonal AF-285-NA (B/N, ICC/IF, WB)
Human Polyclonal AB-285-NA (B/N, WB)
Mouse 37895 MAB485 (B/N, FC, WB)
Mouse 37895R MAB485R (B/N, FC)
Mouse H22 MAB4851 (B/N, ICC/IF)
Mouse Polyclonal AF-485-NA (B/N, WB)
IL-12Human Polyclonal AF-219-NA (B/N, ICC/IF, WB)
Mouse Polyclonal AF-419-NA (B/N, WB)
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Th17 Cells
Molecule Species Source Catalog #
IL-1b/IL-1F2
Human E. coli 201-LB
Mouse E. coli 401-ML
Rat E. coli 501-RL
IL-6
Human E. coli 206-IL
Human HEK293 7270-IL
Mouse E. coli 406-ML
Rat E. coli 506-RL
IL-21Human E. coli 8879-IL
Mouse E. coli 594-ML
IL-23
Human Sf21 (baculovirus) 1290-IL
Mouse Sf21 (stably transfected) 1887-ML
Rat Sf21 (baculovirus) 3136-RL
TGF-b1
Human HEK293 7754-BH
Human CHO 240-B
Human Platelets 100-B
Mouse CHO 7666-MB
Antibodies Species Clone Catalog # (Applications)
CD3Human UCHT1 MAB100 (FA, FC, ICC/IF, IP)
Mouse 145-2C11 MAB484 (Depl., FA, FC, IP)
CD28
Human 37407 MAB342 (FA, WB)
Human Polyclonal AF-342-PB (FA, FC, ICC/IF, IHC)
Mouse 794716 MAB4832 (FA, FC)
IFN-g
Human 25718 MAB285 (B/N, FC, ICC/IF)
Human 25718R MAB285R (B/N, ICC/IF)
Human K3.53 MAB2852 (B/N, Ecap, FC, WB)
Human Polyclonal AF-285-NA (B/N, ICC/IF, WB)
Human Polyclonal AB-285-NA (B/N, WB)
Mouse 37895 MAB485 (B/N, FC, WB)
Mouse 37895R MAB485R (B/N, FC)
Mouse H22 MAB4851 (B/N, ICC/IF)
Mouse Polyclonal AF-485-NA (B/N, WB)
IL-4
Human 34019 MAB204 (B/N, WB)
Human 3007 MAB304 (B/N, ICC/IF, WB)
Human Polyclonal AF-204-NA (B/N, WB)
Mouse 30340 MAB404 (B/N, Ecap, WB)
Mouse Polyclonal AF-404-NA (B/N, WB)
Mouse 30340R MAB404R (B/N, Ecap)
Learn more | rndsystems.com/proteins
Antibody Application KeyB/N Blocking/Neutralization Depl Cell Depletion Ecap ELISA Capture FA Functional Assay FC Flow Cytometry ICC/IF Immunocytochemistry/Immunofluorescence IHC Immunohistochemistry WB Western Blot
To learn about the cell surface and intracellular markers that are most commonly used in the published literature to identify different immune cell types, explore our new Cell Markers Interactive Resource Tool at rndsystems.com/cellmarkers
Th17 Cell Markers
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Animal-Free or Animal Component-Free Process Proteins
Molecule Species Source Catalog #
CD40 Ligand/TNFSF5 Human E. coli AFL617
Flt-3 Ligand Human Sf9 (baculovirus) ACFP308
G-CSF Human E. coli AFL214
GM-CSFHuman E. coli AFL215
Mouse E. coli AFL415
IFN-g Human E. coli AFL285
IL-1b/IL-1F2Human E. coli AFL201
Mouse E. coli AFL401
Animal-Free or Animal Component-Free Process Proteins
Molecule Species Source Catalog #
IL-2 Human E. coli AFL202
IL-3 Human E. coli AFL203
IL-4 Human E. coli AFL204
IL-6 Human E. coli AFL206
M-CSFHuman E. coli AFL216
Mouse E. coli AFL416
TNF-aHuman E. coli AFL210
Mouse E. coli AFL410
ProDots Recombinant Human Proteins
Molecule Catalog #
Flt-3 Ligand PRD308
G-CSF PRD214
GM-CSF PRD215
IFN-g PRD285
IL-1b PRD201
IL-2 PRD202
ProDots Recombinant Human Proteins
Molecule Catalog #
IL-3 PRD203
IL-4 PRD204
IL-6 PRD206
IL-10 PRD217
M-CSF PRD216
TGF-b1 PRD240
For a complete, up-to-date product listing | rndsystems.com/prodots
For a complete, up-to-date product listing | rndsystems.com/animalfree
ProDots® Proteins
ProDots® proteins are R&D Systems proteins packaged into easy-to-use lyophilized dots. They are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue in a vial, ProDots proteins are neatly bundled into the shape of a ball so that they can be rolled directly into cell culture media. The benefits of ProDots proteins include: • Eliminates aliquoting
• Easy cell culture media preparation
• Ensures 100% protein recovery, dissolves instantly
• Avoids freeze/thaw cycles
Animal-Free™ and Animal Component-Free Process (ACFP) Recombinant Proteins
Animal-free proteins are particularly important for researchers concerned with experimental variables caused by trace animal components or mammalian pathogens. To support developing research and bioproduction demands, Bio-techne offers R&D Systems Animal-Free recombinant proteins, which are manufactured in a laboratory exclusively dedicated to the production and purification of proteins under animal-free conditions. We also offer R&D Systems Animal Component-Free Process (ACFP) recombinant proteins that are expressed in an animal-free certified Sf 9 insect cell line using dedicated animal-free raw materials and labware. Features of our Animal-Free proteins include:• Endotoxin Levels < 1.0 EU per 1 µg of product
• Tested to ensure no impairment of biological activity
• Additional documentation can be provided for ex vivo research or bioproduction
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GMP-grade Immune Cell Culture Reagents
As more and more researchers are investigating the potential of different immune cell populations for therapeutic purposes, the need for innovative tools for ex vivo cell manufacturing has grown. To address this need, we continue to expand our portfolio of GMP-grade reagents, which now includes GMP-grade recombinant proteins along with custom cell separation and CD3/CD28 T cell activation kits manufactured under GMP controls.
Cloudz™ Cell Selection Kits
Bio-Techne is utilizing its pioneering Cloudz dissolvable hydrogel to design a cell selection system that enables the gentle capture and release of select cell populations. Using the principles for cell selection, this technology is ideal for simplifying cell manufacturing protocols, while reducing the risk of bead contamination during downstream cell culture processing. This technology is currently available as a custom service. Features of the technology include:• Size exclusion-based cell selection
• Sequential cell selection is available for improved target definition
• Manufactured under GMP controls
• Compatible with multiple clinical expansion platforms
• Customizable for your desired cell population
Principle of the Technology
Step 1: Cloudz particles bind desired cell population
Step 2: Desired cells are retained
Step 3: Cloudz particles are dissolved and desired cells are collected
Learn more | rndsystems.com/cloudzselection
Unwanted cell Desired cell bound toantibody conjugated Cloudz particle
Unwanted cells
Cell strainerDesired cells
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GMP Cloudz™ T Cell Activation Kit – CD3/CD28
The Cloudz T Cell Activation Kit – CD3/CD28 contains non-magnetic, dissolvable microparticles that are manufactured under GMP controls and conjugated with the common T cell-activating antibodies, CD3 and CD28. This kit improves T cell expansion and simplifies cell recovery for ex vivo manufacturing, while also providing safety and efficacy advantages over current bead-based T cell activation methods. Features of the kit include:• Robust T cell activation and expansion
• Simplified recovery of cells – no beads to remove for cell culture
• A release buffer that dissolves particles in < 10 seconds
Custom Services
If you still don’t see what you need, let us know. Bio-Techne offers expert custom cell culture media production services along with custom protein and antibody services to ensure that we can meet your research needs.
GMP-grade Recombinant Proteins
R&D Systems GMP-grade recombinant proteins are manufactured under guidelines that allow for their use as ancillary materials in cell therapy or for further manufacturing processes. They come with extensive documentation and traceability, as well as additional quality control testing. Each GMP-grade protein is tested for performance using cell-based bioassays including proliferation, induced cytokine secretion, cytotoxicity, and others. Whenever possible, our GMP proteins are made in an entirely animal-free process using E. coli as the source, although there are some instances when a protein requires production in a eukaryotic system to maintain activity. The R&D Systems website always clearly indicates whether a specific GMP-grade protein is produced in an animal-free process or not.
Learn more | rndsystems.com/gmp
Learn more | rndsystems.com/custom
GMP-grade Proteins
Molecule Species Source Catalog #
GM-CSF Human E. coli 215-GMP
IFN-g Human E. coli 285-GMP
IL-1b Human E. coli 201-GMP
IL-2 Human E. coli 202-GMP
IL-3 Human E. coli 203-GMP
IL-4 Human E. coli 204-GMP
GMP-grade Proteins
Molecule Species Source Catalog #
IL-6 Human E. coli 206-GMP
IL-12 Human Sf 21 (baculovirus) 219-GMP
IL-15 Human E. coli 247-GMP
M-CSF Human E. coli 216-GMP
TGF-b1 Human CHO 240-GMP
TNF-a Human E. coli 210-GMP
Add ReleaseBuffer
Cloudz dissolve <10 seconds
+101 102 103 104
5
10
25
Rela
tive
Cell
Num
ber
1000
20
15
CD4
Cloudz CD3/CD28 T Cells or PBMC Cloudz/Cell Complex T Cell Expansion Downstream ApplicationsMicrosphere-free T Cells
Learn more | rndsystems.com/cloudzactivation
BR_Immune Cells_RDS19-1447
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