Research has shown our strains offer the following immune benefits:

Supports the immune system by promoting gut comfort and improving gut function(1, 23)

Reduces risk of respiratory disease in infants(28, 38)

May reduce inflammation associated with certain infections(15, 19, 20)

Improves protection against diarrhoea causing infections(14)

BifidoB HN019: Bifidobacterium animalis subspecies lactis HN019

LactoB HN001: Lacticaseibacillus rhamnosus HN001*

Supporting the immune system by improving gut-barrier function(1)

Improves immune bio-markers associated with protection from pathogens(10, 24, 29)

Reduces levels of certain gut(8) and vaginal pathogens(26)

Reduces inflammation associated with raised interleukin 6 levels, a cytokine protein that plays a key role in inflammation response(11)

Immunity-Supporting Ingredients

Telling a credible immunity story starts with trusted ingredients backed by evidence. Here’s what NZMP has to offer.

What are bio-markers?• Bio-markers are anything that can be used to monitor physiological

process, including indicators of disease, e.g. proteins or increasedlevels of certain types of immune cells.

• In clinical studies, our probiotics improved activity of immunecells called ‘Natural Killer Cells’ and phagocytes. These play vital“surveillance” roles in the immune system by recognising pathogens,alerting the rest of the immune system, and removing the threat.

PROBIOTICS NZMP offers 2 probiotic strains, backed by decades of clinical research that demonstrate their safety and effectiveness in providing support for immunity.

The information in this document is designed to give a brief overview of the nature and characteristics of our ingredients as at the date of preparation. It does not constitute representations or warranties as to such characteristics, the functionality or potential applications of our ingredients and you must assure yourself of these matters having regard to our detailed product specifications, your specific circumstances and applicable market regulations. NZMP, the nzmp droplet, and SureProtein™, HN001 & HN019 are trademarks of Fonterra Co-operative Group Limited.

* In April 2020 the Lactobacillus genus was reclassified, resulting in a name change of our probiotic from Lactobacillus rhamnosus HN001 to Lacticaseibacillus rhamnosus HN001. There is no change to the abbreviated scientific notation L.rhamnosus HN001. Additional information can be found here.

• GOS is a type of prebiotic, made of chains ofgalactose, which is derived from the milk sugarlactose.

• A prebiotic is a soluble fibre which humanscannot digest. Instead it is used as food for thebeneficial bacteria (e.g. probiotics) in the gut.

• Prebiotics provide 2 main benefits:

1. Supports the growth of ‘good’ bacteria in thegut(7, 12, 16, 36).

2. When prebiotics are digested by gutmicrobiota, they make other nutrients, suchas short chain fatty acids which supportgut cells(4, 22, 30) and help with mineralabsorption(34, 36).

• Has antioxidant effects(39)

• Can help to modulate aspects of the immune system, including growth of whiteblood cells & some immune signalling molecules(13, 40).

• Supports the health skin and gut lining to reduce the risk of toxins or pathogensentering the body(13).

• Supports some types of immune cells (e.g. Natural Killer cells) and their responseto harmful substances(13, 26).

• Supports protection and function of immune cells, including potential cytotoxicactivity e.g. killing of harmful bacteria(13, 26).

PROTEINS• Milk naturally contains 3.5% protein (80% casein,

20% whey).• Dairy protein is a rich source of bioactive

components including alpha-lactalbumin,immunoglobulins, and lactoferrin.

• Dairy protein is also a rich source of essential aminoacids. These are nutrients which the human bodycannot make, so must be sourced from the diet.

Micronutrients:Several of our protein ingredients naturally contain vitamins and minerals that have a vital role in immunity. For example:

Zinc

Folate

B12

PREBIOTICS: GALACTO-OLIGOSACCHARIDES (GOS)

EnzymeBreaking down

lactose into Glucose & Galactose

Galactose Chain

Lactose Sugar (from milk)

GAL

GAL

GAL

GAL GALGAL

GLC

GLC

GLC

Enzyme

Lactoferrin:One of the largest proteins found in milk, usually making up 1% of Whey Protein.

This protein is naturally bound to iron, giving it a distinctive pink colour.

Lactoferrin has strong antioxidant properties(3), and can provide protection against certain fungal, viral or bacterial infections(5, 17, 21, 32, 34).

It does this by binding with harmful molecules or micro-organisms to:

Damage them e.g. breakdown their cell walls(9, 32)

“starve” them of essential nutrients(2)

Send signals to trigger an immune response(6, 31, 33)

So how does this work?

• Whey is a source of amino acids forGLUTHATHIONE production.

• GLUTATHIONE is a molecule foundthroughout the body, that has a critical rolein the immune system.

• It is made in the liver by the amino acids:glutamate, cysteine and glycine.

• GLUTATHIONE is a powerful antioxidant,which plays a role in protecting cells againstoxidative stress, caused by factors such aspollution, toxins, exercise and poor diet.

• Protecting against oxidative stress is animportant part of our immune response,especially as it is considered an underlyingfactor in numerous diseases.

• Having an adequate supply of GLUTATHIONEis essential to support immunity.

• Whey protein one of the richest sources ofCYSTEINE, so can help to support adequateGLUTATHIONE production.

Whey Protein:

The efficacy of whey in supporting immunity has been shown in numerous cell and animal studies. There is some emerging evidence in human studies - although further research is required.

HO

GLUTATHIONE

Essential for a good immune system(18)

y-Glutamate

NH2 O

OOOHS

OH

Cysteine Glycine

H NN

H

Lactoferrin Protein Structure.

Amino acids are the building blocks of proteins, which

make-up many components of our body, including skin,

muscle, DNA and even immune cells and antibodies.

Talk to the dairy ingredient expertsWe’re passionate about sharing our deep dairy expertise to help you grow your business. Talk to us today about your dairy ingredient needs.

To find out more please contact your account manager or visit nzmp.com

Conclusion There are many different factors that can impact an individual’s immunity, including underlying genetics, environmental factors, lifestyle, and nutrition.

Although dietary factors may not be able to address all specific diseases or conditions, good nutrition can go a long way to supporting and maintaining overall immunity.

NZMP has a range of ingredients, that could help support this story.

Contact us now

1. Anderson RC, Cookson AL, McNabb WC, Kelly WJ, Roy NC (2010) Lactobacillus plantarumDSM 2648 is a potential probiotic that enhances intestinal barrier function. FEMS Microbiology Letters 309:184- 92.

2. Arnold RR, Brewer M & Gauthier JJ. (1980) Bactericidal activity of human lactoferrin:sensitivity of a variety of microorganisms. Infection and Immunity; 28, 893-898.

3. Britigan BE, Serody JS & Cohen MS. (1994) The role of lactoferrin as an anti-inflammatory molecule. Advances in Experimental Medicine and Biology; 357, 143-156.

4. Burger-van PN, , Vincent A, Puiman PJ, van der Sluis M, Bouma J, Boehm G, van Goudoever JB, van Seuningen I, Renes IB. (2009) The regulation of intestinal mucin MUC2 expression by short-chain fatty acids: implications for epithelial protection. Biochemical Journal 420(2)211-219.

5. Conneely OM. (2001) Antiinflammatory activities of lactoferrin. J Am Coll Nutr. 20,389S-395S.

6. Drago-Serrano ME, Campos-Rodriguez R, Carrero JC, de la Garza M. (2017) Lactoferrin: balancing ups and downs of inflammation due to microbial infections. Int J Molecular Sci18:501-26.

7. Duggan C, Watkins JB, and Walker WA. (2008) Nutrition in pediatrics: basic science, clinicalapplications. PMPH-USA.

8. Eggers S, Barker AK, Valentine S, Hess T, Duster M, Safdar N. (2018) Effect of Lactobacillus rhamnosus HN001 on carriage of Staphylococcus aureus: results of the impact of probioticsfor reducing infections in veterans (IMPROVE) study. BMC Infectious Diseases 14:129.

9. Ellison R.T. (1994) The Effects of Lactoferrin on Gram-Negative Bacteria. In: Hutchens T.W., Rumball S.V., Lönnerdal B. (eds) Lactoferrin. Advances in, Experimental Medicine and Biology,vol 357. Springer, Boston, MA.

10. Gill HS, Rutherfurd KJ, Cross ML, Gopal PK (2001) Dietary probiotic supplementation toenhance cellular immunity in the elderly. British Journal of Biomedical Science 57:94-96.

11. González-Hernández LA, Jave-Suarez LF, Fafutis-Morris M, Montes-Salcedo KE, Valle-Gutierrez LG, Campos-Loza AE, Enciso-Gómez LF, Andrade-Villanueva JF (2012) Synbiotic therapy decreases microbial translocation and inflammation and improves immunological status in HIV-infected patients: a double-blind randomized controlled pilot trial. Nutr J 11:90.

12. Haarman, M., Knol, J. (2005) Quantitative Real-Time PCR Assays To Identify and Quantify Fecal Bifidobacterium Species in Infants Receiving a Prebiotic Infant Formula. Applied and Environmental Microbiology 71(5), 2318-2324.

13. Haryanto B., Suksmasari T., Wintergerst E., Maggini S. (2015) Multivitamin supplementationsupports immune function and ameliorates conditions triggered by reduced air quality. Vitam. Miner. 4:1–15.

14. Hemalatha R, Ouwehand AC, Forssten SD, Babu Geddan JJ, Sriswan R, Bhaskar MV, Radhakrishna KV (2014) A community-based randomized double blind controlled trial of Lactobacillus paracasei and Bifidobacterium lactis on reducing risk for diarrhea and fever in preschool children in an urban slum in India. European Journal of Nutrition & Food Safety 4:325-341.

15. Invernici MM, Salvador SL, Silva PHF, Soares MSM, Casarin R, Palioto DB, Souza SLS, Taba M Jr, Novaes AB Jr, Furlaneto FAC, Messora MR. (2018) Effects of Bifidobacterium probioticon the treatment of chronic periodontitis: A randomized clinical trial. Journal of Clinical Periodontology 45:1198-1210.

16. Knol, J., Scholtens, P., Kafka, C., Steenbakkers, J., Gro, S., Helm, K., Klarczyk, M., Schöpfer, H., Böckler, H.-M., Wells, J. (2005) Colon Microflora in Infants Fed Formula with Galacto- and Fructo-Oligosaccharides: More Like Breast-Fed Infants. Journal of Pediatric Gastroenterology and Nutrition 40, 36-42.

17. Legrand D, Elass E, Pierce A, & Mazurier J. (2004) Lactoferrin and host defence: an overview of its immunomodulating and anti-inflammatory properties. Biometals; 17, 225-229.

18. Middleton, N., P. Jelen, and G. Bell. 2004. Whole blood and mononuclear cell glutathione response to dietary whey protein supplementation in sedentary and trained male humansubjects. Int. J. Food Sci. Nutr. 55:131–141

19. Oliveira LF, Salvador SL, Silva PH, Furlaneto FA, Figueiredo L, Casarin R, Ervolino E, PaliotoDB, Souza SL, Taba M Jr, Novaes AB Jr, Messora MR (2017) Benefits of Bifidobacterium animalis subsp lactis Probiotic in Experimental Periodontitis. Journal of Periodontology 88:197-208.

20. Oliveira LFF, Teixeira GC, Pischiotini JP, Moreira ALG, Ervolino E, de Oliveira MN, Bogsan CSB, Salvador SL, Messora MR (2017) Effects of the probiotic Bifidobacterium animalissubsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats. PLoS One. 12:e0179946.

21. Orsi N (2004) The antimicrobial activity of lactoferrin: current status and perspectives.Biometals, 17, 189-96.

22. Pratt VC, Tappenden KA, McBurney MI, Field CJ. (1996) Short-chain fatty acid-supplementedtotal parenteral nutrition improves nonspecific immunity after intestinal resection in rats. Journal of Parenteral and Enteral Nutrition 20(4) 264-271.

23. Putaala H, Salusjarvi T, Nordstrom M, Saarinen M, Ouwehand AC, Hansen EB, Rautonen N (2008) Effect of four probiotic strains and Escherichia coli O157:H7 on tight junction integrity and cyclo-oxygenase expression Research In Microbiology 159:692-698.

24. Quan S, Gill HS. (2002) Immune protection by the probiotic Lactobacillus rhamnosus HN001against Escherichia coli O157:H7 infection in mice. FEMS Immuno. & Med Microbiol. 34:59-64.

25. Ricoldi MST, Furlaneto FAC, Oliveira LFF, Teixeira GC, Pischiotini JP, Moreira ALG, Ervolino E, de Oliveira MN, Bogsan CSB, Salvador SL, Messora MR. (2017) Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats. PLoS One.12:e0179946.

26. Russo R, Terruzzi F, De Alberti D, Nobile V, Terruzzi C, Ouwehand A. (2016) Vaginal Colonization of Lactobacillus acidophilus LA-14 and Lactobacillus rhamnosus HN001 after Oral Administration in Healthy Women. Journal of Clinical Gastroenterology 50:S221-222

27. Saeed F, Nadeem M, Ahmed R, Nadeem M, Arshad M, Ullah A. (2016) Studying the impactof nutritional immunology underlying the modulation of immune responses by nutritional compounds—A review. Food Agric. Immunol. 27:205–229.

28. Sazawal S, Dhingra U, Hiremath G, Sarkar A, Dhingra P, Dutta A, Verma P, Menon VP, Black RE. (2010) Prebiotic and probiotic fortified milk in prevention of morbidities among children:community-based, randomized, double-blind, controlled trial. PLoS One. 5:e12164.

29. Sheih YH, Chiang BL, Wang LH, Liao CK, Gill HS. (2001) Systemic immunity enhancing effects in healthy subjects following dietary consumption of lactic acid bacterium Lactobacillus rhamnosus HN001. Journal of the American College of Nutrition 20:149- 156

30. Suzuki T, Yoshida S, Hara H. (2008) Physiological concentrations of short-chain fatty acids immediately suppress colonic epithelial permeability. British journal of nutrition 100 (2) 297-305.

31. Teraguchi S, Wakabayashi H, Kuwata H, Yamauchi K, & Tamura Y. (2004) Protection againstinfections by oral lactoferrin: Evaluation in animal models. Biometals; 17, 231-234.

32. Tomita M, Takase M, Wakabayshi H, Bellamy W. (1995) Antimicrobial peptides of lactoferrin,Adv Exp Med Biol 357:108,

33. Tomita M, Wakabayashi H, Yamauchi K, Teraguchi S. & Hayasawa H. (2002) Biochemistry Cell Biology 80, 109-112.

34. Valenti P, Berlutti F, Conte MP, Longhi C, & Seganti L (2004) Lactoferrin functions. Currentstatus and perspectives. J Clin Gastroenterol. 38, S127-S129.

35. Van den Heuvel, Ellen GHM, Margriet HC Schoterman, and Theo Muijs. (2000) Transgalactooligosaccharides stimulate calcium absorption in postmenopausal women. TheJournal of nutrition 130 (12) 2938-2942.

36. Vulevic J, Drakoularakou A, Yaqoob P, Tzortzis G, Gibson GR. (2008) Modulation of the fecal microflora profile and immune function by a novel trans-galactooligosaccharide mixture (B-GOS) in healthy elderly volunteers. The American Journal of Clinical Nutrition 88(5) 1438-1446.

37. Whisner CM, Martin BR, Schoterman MHC, Nakatsu CH, McCabe LD, McCabe GP, Wastney ME, van den Heuvel EG, Weaver CM. (2013) Galacto-oligosaccharides increase calcium absorption and gut Bifidobacteria in young girls: a double-blind cross-over trial. Brit J Nutr 110, 1292-1303.

38. Wickens K, Barthow C, Mitchell EA, Stanley TV, Purdie G, Rowden J, Kang J, Hood F, van den Elsen L, Forbes-Blom E, Franklin I, Barnes P, Fitzharris P, Maude RM, Stone P, Abels P, Murphy R, Crane J. (2018) Maternal supplementation alone with L. rhamnosus HN001 during pregnancy and breastfeeding does not reduce infant eczema. Pediatric Allergy andImmunology 29:296-302.

39. Wintergerst E., Maggini S., Hornig D. (2006) Immune-enhancing role of vitamin C and zincand effect on clinical conditions. Ann. Nutr. Metab.;50:85–94.

40. Wintergerst E., Maggini S., Hornig D. (2007) Contribution of selected vitamins and traceelements to immune function. Nutr. Metab. 51:301–323.

REFERENCES