immuno ppt final

8/10/2019 Immuno Ppt Final

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2/24

I%trodu2tio% Te Po3#.2i!. b!34ouri!%!

'A%dr5, L6H6B!i3e# '(7(0, i.%!ti"e to Au.tr!3i!'8uee%.3!%d, !%d P!&u! Ne9Gui%e!6Te %!me o4 te$e%u. 2ome. 4rom te2ombi%!tio% o4 te Gree/9ord. &o3#.; < mu2 !%d

./i!; < .!de= 9it &o..ib3ere4ere%2e to te ti2/ 4o3i!$e>te .&e2ie. i. o%oured tote E%$3i. bot!%i.t ?o%

Hutto% B!34our '(@*@(@@,6


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C


4/24

He.&eridi%I.or!m%eti%-!em&4ero3M#ri2eti%N!ri%$i%

Nobi3eti%Pro!%to2#!%idi%.8uer2eti%Ruti%

T!%$ereti%E&i2!te2i%

C!&.!i2i%E33!$i2 A2idG!33i2 !2idRo.m!ri%i2 !2id

T!%%i2 A2id

AnthocyaninsC#!%idi%M!3"idi%

C cor c ac

Coumarin

Ferulic acid

Scopoletin

Alkaloids

Caffeine

Theobromine Theophylline

CarotenoidsBet!C!rote%eLutei%L#2o&e%e

PhytosterolsBet!Sito.tero3

TriterpenoidsUr.o3i2 !2id

Isoflavones

Daidzein

Genistein

Lignans

Silymarin

Monophenols

Saponins XanthophyllsA.t!!%ti%Bet!Cr#&to!%ti%


5/24

Corre3!tio% bet9ee% to2emi2!3.!%d &!rm!2o3o$i2!3 &ro&ertie.

&e%o3i2 2o%te%t te !%ti oid!%t &ro&ertie. 'Li et6!36 )**@,'-um!r!% et6!3 )**+, 'C!teri%e et6!36 (77+,

&3!%t .tero3. to.tero3. b3ood 2o3e.tero3 3e"e3 3o9eri%$!$e%t.6 'D!"id !%d Sir3e# )**,

F3!"!%oid. !%ti b!2teri!3= !%ti "ir!3= !%ti 4u%$!3 !2ti"itie.6A%ti 4u%$!3 !2ti"it# i. due to te !bit3it# to i%ibit .&ore

$ermi%!tio%6'Or%er !%d ! (771,

A%ti "ir!3 !2ti"it# i. due to te !bi3it# o4 .ome J!"!%oid. to i%ibit

re"er.e tr!%.2ri&t!.e6 'Li! et6!36 )**),

A%ti b!2teri!3 !2ti"it# o22ur. due to te i%ibitio% o4 %u23ei2 !2id.#%te.i.= i%ibitio% o4 2#to&3!.mi2 membr!%e 4u%2tio% !%di%ibitio% o4 e%er$# met!bo3i.m6'L!mb !%d 2u.%ie )**,


6/24

P3!%t .!&o%i%. !re $3#2o.ide.!"i%$ A%ti i%J!mm!tor#=emo3#ti2= !%ti tumor=2#totoi2= !%ti mo33u.2id!3= !%ti4u%$!3= !%ti b!2teri!3!2ti"itie. 6 'S&!r$ et6!36 )**,

Ter&e%oid. 3i/e o3e!%o3i2 !2id!%d ur.o3i2 !2id !"e bee%e.t!b3i.ed to !"e !%tii%J!mm!tor# !%d !%ti#&er3i&idemi2 !2ti"it#6 'ie 3iu(77,

A3/!3oid. !"e bee% .tudiedm!i%3# 4or teir eKe2t. o% te2e%tr!3 %er"ou. .#.tem6 Te#eibit !%ti2o3i%e.ter!.e

!2ti"it#6 'E3!i%e et6!36 (77,


7/24

PREVIOUS WORK Immu%o.timu3!%t !2ti"it# 9!. .tudied b#

&er4ormi%$ 2!rbo% 23e!r!%2e te.t !%d mi3/i%du2ed 3eu/o2#to.i.6 Te re4ere%2e 9!. &!%!$i%.e%$ root &o9der6 Te# 4ou%d out t!t teroot &o9der o4 te &3!%t di.&3!#ed better2!rbo% 23e!r!%2e !t ! do.e o4 **m$/$ !%d!%ti 3eu/o2#te !2ti"it# !t ! do.e o4 (10m$/$t!% oter 3e!4 !%d root etr!2t.6 'S!%d#!et6!3 )*(*,

A%ti u32er !2ti"it# o4 te 3e!4 !%d root etr!2t.9!. .tudied i% 2om&!ri.io% 9it ! .t!%d!rd i6e$i%.e%$ root &o9der6 Le!4 etr!2t. o4 do.e**m$m3 bod# 9ei$t .o9ed &romi.i%$ !%tiu32er !2ti"it#6 'S!%d#! et6!3 )*(*,


8/24

Obe2ti"e.

To &re&!re &3!%t etr!2t 4rom&6b!34ouri!%! m!r$i%!t! 3e!"e. u.i%$.o3et etr!2tio%6

S2ree%i%$ 4or "!riou. P#to2emi2!3'ter&e%oid.= J!"o%oid. et2, i% teetr!2t6

S2ree%i%$ 4or !%ti b!2teri!3 !2ti"it#6


9/24

Metod. !%d m!teri!3.reuired

Plant aterial:(6Te reuired !mou%t o4 3e!"e. 9ere

2o33e2ted !%d dried i% te mi2ro9!"e

o"e% 4or () r. !t * de$ 2 6


10/24

)6Dried 3e!"e. 9ere &o9dered i% teb3e%der !%d + $m o4 te &o9der9!. t!/e% 4or te etr!2t&re&!r!tio%6


11/24

E!tractpreparation"

(6So3et etr!2tio%9!. &er4ormed 9it tedried !%d &o9dered

3e!"e. '+$, u.i%$met!%o3 !. te.o3"e%t6


12/24

)6Te obt!i%ed etr!2t 9!. !ir dried!%d !3.o 9!terb!t 9!. u.ed toremo"e te .o3"e%t6 A%d te% it 9!.

L#o&i3i.ed to remo"e !%# .o3"e%t2o%te%t m!i%3# met!%o3 9i2 9!.u.ed 4or te .o3et etr!2tio%6

Yield obtained = 100* mass ofextract/mass of dried leaves< (** '+6(7 +,

< *6((** or (


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14/24


15/24

P#to2emi2!3 te.t.

#$Test %or Saponins&'oa( test) 'C!r3e.et6!36 )*(1,

To )m3. o4 etr!2t 9!.

!dded 0m3 o4 9!ter i%! te.t tube6 Temiture 9!. .!/e%

"i$orou.3# !%dob.er"ed 6 4orm!tio% o4 &er.i.te%t

4o!m t!t 2o%rm. te

&re.e%2e o4 .!&o%i%.


16/24

*$Test %or Terpenoids&Sal+o,+i-s test)'C!r3e. et6!36 )*(1,

(m3 o4 23oro4orm 9!.!dded to )m3 o4etr!2t 4o33o9ed b# !

4e9 dro&.o42o%2e%tr!ted .u3&uri2!2id6

reddi. bro9%&re2i&it!te &rodu2edimmedi!te3# i%di2!tedte &re.e%2e o4

ter&e%oid.


17/24


18/24

0$Test %or Phenols&'erric chloridetest) 'C!r3e. et6!36

)*(1, A 4r!2tio% o4 te

etr!2t. 9!.

tre!ted 9it!ueou. 4erri223oride !%d

ob.er"ed 4or4orm!tio% o4 dee&b3ue or b3!2/ 2o3our6

No dee& b3ue or


19/24

A%ti B!2teri!3 !..!#

A%ti b!2teri!3 !2ti"it# o4 te 3#o&i3ied etr!2t 9!.te.ted b# u.i%$ 23or!m&e%i2o3 !. te 2o%tro3!%tibioti2 i% A$!r 9e33 di3utio% or A$!r 9e33 diKu.io%metod6

Tree &!to$e%i2 .tr!i%.= E2o3i= B!2i33u. !%dP.eudomo%!. !eru$i%o.! 9ere obt!i%ed 4rom te.2oo3 o4 bio.2ie%2e. !%d te2%o3o$#= VitU%i"er.it#= Ve33ore6

Te 2u3ture. 9ere re"i"ed i% Luri! bert!%ii brot6 (m3 o4 te re"i"ed 2u3ture i. i%o2u3!ted i%to (** m3o4 4re. Luri! bert!%ii brot !%d i%2ub!tedo"er%i$t !t 1+Q C6


20/24


21/24

Tree Petri &3!te. 9ere m!de 9it Mue33er Hi%to% !$!r!4ter .teri3i!tio%6

(* 3 i%o22u3um o4 e!2 &!to$e% .tr!i% 2u3ture 9!.t!/e% u.i%$ .teri3e &i&ette ti&. !%d u.i%$ ! .teri3e 2otto%

.9!b te 2u3ture 9!. .&re!d o% te tree &3!te.6 0 e33. 9ere 2ut i% te !$!r u.i%$ ! 2or/ or $e3 borer o4

di!meter +mm6

te etr!2t 9!. di..o3"ed i% 9!ter to m!/e ! (m$m3.o3utio%6 From ti. .o3utio% )*3= * 3= 0* 3= @* 3 !%d

(** 3 9ere t!/e% !%d &i&etted o%to te &3!te. i% !3!mi%!r !ir Jo9 i% te re.&e2ti"e 9e33.6

Te &3!te. 9ere i%2ub!ted i% i%2ub!tor !t 1+Q Co"er%i$t !%d te o%e o4 i%ibitio% 9!. ob.er"ed6


22/24

Re.u3t.Phytochemical Test Result

Phenols Ferric chloride test -ve

Flavanoids l!aline rea"ent test -ve

Terpenoids Sal!o#!i$s test % ve

Saponins Foam test % ve


23/24


24/24

Co%23u.io%

Te 3!2/ o4 !%tib!2teri!3 !2ti"it# i% te &3!%tetr!2t 2!% be 2o%tributed to 4o33o9i%$ 4!2tor.6

(6 Te J!"!%oid 2o%te%t i% te &3!%t etr!2t i.

"er# 3o9 !. obt!i%ed 4rom te to2emi2!3te.t6

)6 E"e% tou$ .!&o%i% 9!. dete2ted i% ouretr!2t6 S!&o%i% 2!% !2t !. !%tib!2teri!3

!2ti"it# i% 3o9 2e33 de%.it#6 Si%2e te i%o2u3umu.ed 9!. i% more !mou%t =te !%tib!2teri!3!2ti"it# 9!.%t dete2ted6

immuno ppt final

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