in vivo molecular imaging at cellular resolution · s-300, neuropak™ camk–egfp transgenic mice...

In Vivo Molecular Imaging at Cellular Resolution

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Image neurons at work, anywhere in the brainwith

EUROPEMauna Kea Technologies SA

9 rue d’Enghien,75010 Paris, France

Tel: + 33 1 48 24 03 45Fax: + 33 1 48 24 12 18

USAMauna Kea Technologies, Inc.3240 South White Road, PMB#122San Jose, CA 95148Ph : 408-729-3155Fax : 408-729-1889

STRIATUMS-300, NeuroPak™CaMK–eGFP transgenicmice somata of medium spiny neurons

HIPPOCAMPUSS-300, S-650, NeuroPak™AAV transfected neurons

CORTEXS-1500, Mini-Z,FITC injection,Microcirculation

SUBSTANTIA NIGRAS-650, Mini-Z, Oregon Green BAPTA-1, ParkinsonNeuroPak™

ACCUBENSNeuroPak™, S-300,S-650, Addiction

VTAS-300, S-650, NeuroPak™

Lentivirus-transfectedneurons and individual

dendrites

VISUAL CORTEXS-650, Mini-Z, Oregon Green,

BAPTA-1, Neuron activation

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flyer-Neuro corrections (2.indd 1 22/08/12 16:50

IN VIVO MOLECULAR IMAGINGAT CELLULAR RESOLUTION



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Probe-based Confocal Laser Endomicroscope (pCLE) designed for in vivo Molecular Imaging of cellular to subcellular processes at two wavelengths.



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Features

•Dual Band Excitation and Detection• In vivo and in situ Imaging

•Resolution up to 1,4 µm•Real-time smooth video recording at 9-50 fps

•A wide range of microprobes tailored to your application

• Monitor physiological interactions in vivo with minimal invasiveness• A large set of new applications including:Cancer and angiogenesis, neurobiology, inflammation,

immunology, biodistribution, colocalization studies...

•Easy to use and to install•Cutting-edge image processing

•Quantification features, Kinetics analysis•New, intuitive graphical interface

•Operating system: Linux-based, flexible



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INSTRUCTIONS FOR USE en Cellvizio Dual Band Instructions for Use, Part C – February 2012

CELLVIZIO DUAL SOFTWARE

Screen Display

Menu bar

Tool buttons

C - 10/22

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6 7 9

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Technical Specifications

Excitation Wavelengths Laser 1: 488 nm Laser 2: 660 nm

Collection Bandwidths 502 - 633 nm 673 - 800 nm

Temporal Resolution 9 - 50 frames per second9 - 50 frames per second9 - 50 frames per second

Image Export .png, .bmp, . jpeg, .tiff, .mhd.png, .bmp, . jpeg, .tiff, .mhd

Movie Export .avi, .mpeg, .mp4, .swf, .avi, .mpeg, .mp4, .swf, .avi, .mpeg, .mp4, .swf,

Laser Class 2M2M

Dimensions and Weight 310 x 480 x 525 mm, 35 kg310 x 480 x 525 mm, 35 kg310 x 480 x 525 mm, 35 kg



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Laser Scanning UnitConfocal microscope

488 and 660 nm simultaneous illumination

Real-time integration

Confocal ProcessorReal Time processing

Cellvizio Dual SoftwareAcquisition, QuantificationImage Processing

Confocal MicroprobeFiber-optics bundleFlexibleMiniaturized High-end objectives

Confocal Microprobes

* Beveled or Flat tip

Model ApplicationsTip

Diameter (mm)

Lateral Resolution

(µm)

Optical Sectioning

(µm)

Working Distance

(µm)

Max Field Of view

(µm)

Deep brain imaging, designed for permanent implantation on freely moving mice

Part of the NeuroPak™ solution

0,350,47 3,3 15 0 325

Brain, deep brain in mice, other organs at depth if low invasiveness is mandatory 0,3 3,3 15 0 300

Surface Imaging

Brain, deep brain in rats, other organs at depth if low invasiveness is mandatory 0,65 3,3 15 0 600

General applicability, can be used to check fluorescence sensitivity in most targets 1,5 3,3 15 0 600

Vessels, angiogenesis, cell fate, cell morphology, utility depends on cell layer thickness and invasiveness

2,6 1,4 10 60 240

Hi-ResImaging

Vessels, angiogenesis, cell fate, cell morphology, utility depends on cell layer thickness and invasiveness

4,2 1,4 10 30 240

ImagingVessels, angiogenesis, cell fate, cell morphology, utility depends on cell layer thickness and invasiveness

4,2 1,4 10 100 240

Blood flow through the vessel (without penetration) image deeper cell layers of tumor, organ or tissue

1,8 3,5 70100/170

at488/660

600

Depth Imaging

Cavities, eye 0,94 3,5 30

50/70at

488/660 325

SSeries

MSeries

S-300*

S-0650*

S-1500

UltraMiniO

MiniO/30

MiniO/100

Z-1800

Mini-Z

ZSeries

CerboFlex™CerboFlex™ J



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Applicative Outcomes

Real-time videos acquired on a tumor xenograft implanted on a nude mouse

Cellvizio Dual Band provides unambiguous coregistered signal from two different fluorochromes.

FITC Dextran was injected as a vessel indicator, combined with AngioStamp®, a molecular marker that targets specificly AvB3 integrin, an endothelial pattern overexpressed in tumor neovessels.

Examples of early results obtained in vivo illustrated on the following figures :

Access to both functional and anatomical information in vivo

> Imaging of vessels and macrophages

Monitoring of the excretion process into the kidney

> Glomeruli and convoluted tubules visualized

CONFIDENTIAL - NOT FOR DISTRIBUTION

3 hours after injection, renal elimination can be appreciated- FITC Dextran (Green)- AngioStamp® (Red)



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Courtesy of F. Duconge, CEA, Orsay, France

in vivo molecular imaging at cellular resolution · s-300, neuropak™ camk–egfp transgenic mice...

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