CYTOCHROME P-45O

BIOCHEMISTRY AND BIOPHYSICS

EDITORS: A.I. ARCHAKOV

G.I. BACHMANOVA

PROCEEDINGS OF THE 7TH INTERNATIONAL

CONFERENCE ON BIOCHEMISTRY AND BIOPHYSICS OF*

CYTOCHROME P"45O: STRUCTURE AND FUNCTION,

BLOTECHNOLOGICAL AND ECOLOGICAL ASPECTS

HELD IN MOSCOW, RUSSIA

JULY 28 - AUGUST 2, 1991

INCO - TNC

JOINT STOCK COMPANY

1992

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CONTENTS

PREFACE Hi

STRUCTURE. CHEMICAL AND MATHEMATICAL MODELS OF

CYTOCHROME P-450 AND ITS DYNAMICS IN CATALYSIS

P. ANZENBACHER, J. HUDECEK, M. STIBOROVA, C. LARROQUE,R. LANGE, G. HEIBEL, P. HILDEBRANDTCOMPARISON OF THE STRUCTURES OF DIFFERENT CYTOCHROMESP-450 AS REVEALED BY EXPERIMENTAL AND THEORETICALMETHODS 1

J.H. DAWSON, B.K. HAWKINS, S. KADKHODAYAN, D.MARYNIAK. T.A. BRYSONSTRUCTURE-FUNCTION RELATIONS OF CYTOCHROMEP-450 AND RELATED HEME ENZYMES 9

S. GRAHAM-LORENCE, S. TUCK, J. FRUETEL, P.R.ORTIZ DE MONTELLANOMOLECULAR MODELING OF THE ACTIVE SITES OFCYTOCHROMES P450 15

G. HUI BON HOA, C. DI PRIMO, E. DEPREZCONFORMATIONAL DYNAMICS OF CYTOCHROME P-450 CAM ASRELATED TO THE BINDING OF THE SUBSTRATE 21

Y. IMAI, T. FUKUDA, M. KOMORI, M. NAKAMURASITE-DIRECTED MUTAGENESIS OF THE CONSERVED THREONINELOCATED AT THE DISTAL-HEME SURFACE FROM P450 II C2,II C14, AND II El 27

C. JUNG, R. SCHOLL, H. FRAUENFELDER, G. HUI BON HOASTRUCTURAL MULTIPLICITY IN THE ACTIVE CENTEROF CYTOCHROME P-450 33

A.M. KHENKIN, A.B. SOROKIN, A.E. SHILOV,MECHANISM OF ALKANE OXIDATION CATALYZED BYCYTOCHROME P-450 AND ITS MODELS 39

T. SHIMIZU. A.J.M. SADEQUE, G.N. SADEQUE, T. TATEISHI,M. ISHIGOOKA. K. HIROYA, M. HATANO, Y. FUJII-KURIYAMAPROTEIN ENGINEERING OF CYTOCHROME P450d (P450IA2) 45

0. ADALI, E. ARINCFURTHER CHARACTERIZATION OF SHEEP LUNG CYTOCHROMEP450LGM2 51

Y. AOYAMA. Y. YOSHIDA, Y. SONODA, Y. SATOSTRUCTURE OF SUBSTRATE ESSENTIAL FOR INTERACTINGWITH LANOSTEROL 14-a-DEMETHYLASE (P-45014DM) 54

A.A. CHERNOGOLOV, T.B. ADAMOVICH, S.A. USANOVIMMUNOCHEMICAL MAPPING OF FERREDOXIN-BINDING SITES INCYTOCHROME P-450scc 57

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A.A. CHERNOGOLOV, A.G. LAPKO, S.A. USANOVSTRUCTURAL AND IKMUNOCHEMICAL ANALYSIS OF CYTOCHROMEP-45O110 FROM BOVINE ADRENAL GORTEX MITOCHONDRIA 60

S.N. GILEVICH, O.L. GURYEVEVALUATION OF ELECTRON TRANSFER DISTANCES IN CYTOCHROMEP-45Oscc AND ADRENODOXIN FROM ADRENAL CORTEX MITOCHONDRIA 63

O.L. GURYEV, S.N. GILEVICH, V.L. CHASHCHINPHENAZINE DYES AS ELECTRON TRANSFER-SHUNTING PROBES IN THERECONSTITUTED CHOLESTEROL HYDROXYLASE 66

T.V. KNYUSHKO, YA.M. COEN, D.R. DAVYDOV, G.P. KU2NETS0VA,E.D. SKOTZELYASFUNCTIONAL PROPERTIES OF CYTOCHROME P45O LM2 MODIFIED BYSUCCINIC ANHYDRYDE 69

R. LANGE, A. PANTALONI, F. BANCEL, C. LARROQUETHERMODYNAMIC INVESTIGATION OF THE CYTOCHROME P-45Oscc SPINSTATE EQUILIBRIUM 72

S. MARDANIAN, Y. SARKISSOVATHE INFLUENCE OF CHEMICAL MODIFICATION BY DIETHYLPYRO-CARBONATE ON THE SUBSTRATE BOUNDING ABILITY OFCYTOCHROME P-45Oscc 75

W.-D. WOGGON, H. PATZELTSYNTHETIC ANALOGUES OF THE ACTIVE SITE OF CYTOCHROME P-450 78

OXYGEN AND SUBSTRATE ACTIVATION BY CYTOCHROME P-450

T. KITAGAWA, T.EGAWA, T. OGURA, Y. IMAI, R. MAKlNO,Y. ISHIMURARESONANCE RAMAN STUDIES OF CYTOCHROME P-450: THE 0-0STRETCHING FOR P-450 CAM AND THE Fe-CO STRETCHING FORP-450(u-l) MUTANTS 83

J. COLLINS, D.L. CAMPER, G.H. LOEWSTEREOSPECIFICITY OF CIS-0-METHYLSTYRENE EPOXIDATlONBY WILD-TYPE AND MUTANT CYTOCHROME P450cam:THEORETICAL STUDIES . 89

S. IKUSHIRO, S. KOMINAMI S., T. YAMAZAKI, S. TAKEMORICONTROL MECHANISM OF P-450 (110) DEPENDENT CATALYTICACTIVITY IN ADRENAL MITOCHONDRIA 95

I.A. SLEPNEVA, V.I. YELINOVA, L.M. WEINERSTRUCTURAL AND FUNCTIONAL ASPECTS OF CHEMICALMODIFICATION OF NADPH-CYTOCHROME P-450 REDUCTASE 101

Y. YOSHIDA, Y. AOYAMA, Y. SONODA, Y. SATOSTRATEGY OF P-450 TO ACHIEVE C-C BOND CLEAVAGE 107

F.E. BEYHL, D.G. MAYEREFFECTS OF MESITYLENE ON MICROSOMAL DRUG-METABOLIZING LIVERENZYMES OF THE RAT IN VITRO 113

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S. DIKALOV, G. RUMYANTSEVAOH-RADICAL GENERATION BY Fe QUINONE-CHELATOR 116

V.V. DUBCHENKOPECULIARITIES OF COMBINED EFFECT OF HYPERBARIC OXYGENATIONAND DRUGS ON THE CYTOCHROME P-450 ACTIVITY IN THE LIVERDURI1NG ACUTE AND CHRONIC ALCOHOLIC INTOXICATION . 119

M. HUSZKA, F. TEICHMANNEFFECTS OF THE NEW ANTIARRHYTHMIC RESTACORIN ON THEMICROSOMAL MONOOXYGENASE SYSTEM 122

I. KARUZINA, D. MENGAZETDINOVCYTOCHROME P-450 INACTIVATION DURING CATALYTIC TURNOVER 130

W.N. KUEHN-VELTENINDIRECT APPROACHES TO ESTIMATE THE ENZYME-BOUND OFA METABOLIC INTERMEDIATE FROM KINETIC AND PARTITIONSTUDIES: RETENTION OF HYDROXYPROGESTERONE BY TESTICULARCYTOCHROME P450XVII 133

P. LUKIENKO, M. BUSHMA, L. LEGONKOVA. G. ABAKUMOV,I. ZVERINSKY, L. ZAVODNIKFOLIC ACID AS NATURAL FACTOR TO MAINTAIN FUNCTION OF LIVERMONOOXYGENASE AND GLUCORONOSYL-CONJUCATING SYSTEM 136

M. MANNO, R. FERRARA, S. CAZZAROINACTIVATION OF HUMAN CYTOCHROME P-450 BY POLYHALOGENATEDCHEMICALS 139

I. MOKHOSOEV, I. KARUZINAOXIDATIVE DEGRADATION OF DITHIONITE-REDUCED CYTOCHROMESP-450 LM2 AND LM4 142

I. MOKHOSOEV. I. KARUZINA, YA. KOENMICROSOMAL PROTEIN DEGRADATION CAUSED BY OXIDATIVE STRESS 145

S.N. MORALEV, B.N. KORMILITSYN, N.M. LIBMAN, V.I. ROZENGART,S.M. RAMSHA SEARCH FOR CYTOCHROME P-450-DEPENDENT MONOOXYGENASEINHIBITORS AMONG ACETYLENIC ARYLALKYLAMINES FOR DESIGN OFPESTICIDE SYNERGISTS 148

S.J. PERNECKY, X. DING, M.J. COONCOMPARISON OF CATALYTIC AND SPECTRAL PROPERTIESOF RABBIT P-450S 2E1 AND 2E2 151

V.E. RYABININ, R.I. LIFSHITSCYTOCHROME P-450 INACTIVATION BY LIPID PEROXIDATION INPATHOLOGY STATE 155

V.S. SHUGALEY, S.I. SADEKOVA, A.A. ANANYAN, N.P. MILUTINAOXYGEN AND CYTOCHROME P-450 ACTIVITY IN RAT LIVER 158

V.V. SHUMYANTZEVA, M.YU. POKROVSKAYA, YU.D. IVANOV,A.A. IZOTOV, V.YU. UVAROVINTERACTION BETWEEN PHOSPHORCORGANIC ANALOGUES OFBIOLOGICALLY ACTIVE COMPOUNDS AND CYTOCHROME P-450 162

Y. SONODA, H. SONO. Y. SATOPURIFICATION AND CHARACTERIZATION OP CYTOCHROME P-45O14DM(LANOSTEROL 14a-DEMETHYLASE) FROM PIG LIVER MICROSOMES 165

A.A. SUVOROV, B.N. KORMILITSYN, S.N. MORALEV, I.V. VOZNY,A.A. KUZNETSOVA STUDY OF CYTOCHROME P-450 EFFECTORS: PHTHALIMIDOAZIMINESAS A NEW CLASS OF MONOOXYGENASE INHIBITORS 168

L. TAN, V.C.O. NJAR, S. KUMARESAN, H.L. HOLLAND6-OXIMINOANDROSTENEDIONE AND 6-OXIMINOTESTOSTERONE: NEWACTIVE SITE-DIRECTED INHIBITORS OF HUMAN PLACENTALAROMATASE 171

H. THOMAS, E. MOLITOR, H. KUSTER, C. SAVOY, C.R. WOLF,F. WAECHTERHYDROXYLATION OF LAURIC ACID AND TESTOSTERONE BY RAT LIVERCYTOCHROME P45OIVA PROTEINS IN RECONSTITUTED SYSTEMS 174

M. TSUBAKI, S. YOSHIKAWA, N.-T. YUEFFECTS OF CHOLESTEROL SIDE-CHAIN GROUPS AND ADRENODOXIN-BINDINGS ON THE VIBRATIONAL MODES OF CARBON MONOXIDE BOUND TOCYTOCHROME P-450scc: IMPLICATIONS OF THE PRODUCTIVE AND THENONPRODUCTIVE SUBSTRATE BINDINGS 177

YA.YU. WOLDMAN, S. KITANOVARADICAL PATHWAY IN OXIDATION OF SOME CYTOCHROME P-450SUBSTRATES IN RAT LIVER MICROSOMES 180

G. ZHIRNOV, V. LISITSYNA, I. SEVRUKOVA, N. SHIPULINA3,4-BENZPYRENE OXIDATION IN SOLUBLE RECONSTITUTEDMONOOXYGENASE SYSTEM AND MICROSOMES 183

G. ZHIRNOV. V. LISITSYNA. N. SHIPULIMA, N. TOMILINA,THE IMMUNOGENIG PROPERTIES OF THE 3,4-BENZPYRENE-ALBUMINCONJUGATE SYNTHESIZED IN THE RABBIT MICROSOMAL MONOOXYGENASESYSTEM 186

G. ZHIRNOV, V. LISITSYNA, N. SHIPULINA. N. TOMILINA.THE IMMUNOGENIC PROPERTIES OF THE CONJUGATES 3,4-BENZPYRENEWITH ALBUMIN OR MICROSOMES 189

A.A. ZHUKOV, J. BLANCK. 0. RISTAU, K. RUCKPAUL, H. REIN,JA.M. KOEN, E.D. SKOTSELYAS, G.P. KUZNETSOVARELATIONSHIP BETWEEN THE SUBSTRATE-INDUCED HIGH SPIN SHIFTOF CYTOCHROME P-450 FERRIC HEME AND THE PARTITION BETWEENPRODUCTIVE AND ABORTIVE MECHANISMS 192

M.G. YAKUBOVSKAYA, G.A. BELITSKYARYL HYDROCARBON HYDROXYLASE INDUCTION IN ASCITES ANDSOLID TUMORS AND TUMOR CELLS IN VITRO 195

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PHOSPHOLIPID-PROTEIN AND PROTEIN-PROTEIN INTERACTIONSIN CYTOCHROME P-450-CONTAINING SYSTEMS

G.I. BACHMANOVA, I.P. KANAEVA, D.R. DAVYDOV, O.V.NIKITJUK, I.R. DEDINSKII, A.G. KRAINEVLIVER MICROSOMAL MONOMERIC RECONSTITUTED SYSTEM:STRUCTURE AND PROPERTIES 198

R. BERNHARDT, R. KRAFT, M. ALTERMAN, A. OTTO, H. SCHRAUBER,I.C. GUNSALUS, K. RUCKPAULCOMMON MECHANISM OF INTERACTION BETWEEN CYTOCHROME P-450 ANDELECTRON DONORS IN DIFFERENT MONOOXYGENASE SYSTEMS 204

M.J. COON, X. DING, J. R. LARSON, S.J. PERNECKY,T.D. PORTERALCOHOL-INDUCIBLE FORMS OF CYTOCHROME P-450:CATALYTIC AND MEMBRANE-BINDING PROPERTIES 210

P. HLAVICAANALYSIS OF THE MODE OF INTERACTION OF NADPH-CYTOCHROME P-450 REDUCTASE AND CYTOCHROME bs

WITH CYTOCHROME P-450LM2 (P-450IIB4) 216

S. KAWATO, Y. OHTAELECTRON TRANSFER INTERACTIONS OF CYTOCHROME P-450IN LIVER AND ADRENAL CORTEX MEMBRANES 222

P.A. KISELEV, A.A. AKHREM. S.A.E. FINCH, V.KRUEGER, A. STIERDYNAMICS OF PROTEIN-LIPID INTERACTION AND CATALYTICACTIVITY OF A LIVER MICROSOMAL MONOOXYGENASE ENZYMESYSTEM 228

C. RICHTER, H. ETTER S. KAWATOROTATION AND INTERACTION WITH EPOXIDE HYDRASE OFCYTOCHROME P-450 IN PROTEOLIPOSOMES 234

J.B. SCHENKMAN, A. VOZNESENSKY, A.I. ARCHAKOVINTERACTION BETWEEN NADPH-CYTOCHROME P450 REDUCTASEAND LM2 (CYP2B4) 240

A. STIERDYNAMICS OF OLIGOMER STRUCTURE IN MONOOXYGENASESYSTEMS 248

H. STROBEL, S.J. SHEN, S. NADLERELECTROSTATIC COMPONENTS IN CYTOCHROME P450 REDUCTASE:CYTOCHROME P450 INTERACTION 254

V.YU. UVAROV, K.N. DEGTYARENKO, I.G. RUKAVISHNIKOVTHE ROLE OF LIPID-PROTEIN INTERACTIONS IN REGULATIONOF CYTOCHROMES P-450 AND EPOXIDE HYDROLASE ACTIVITIES 260

W.G. BALVERS, M.G. BOERSMA, I.M.C.M. RIETJENSSTUDIES ON THE INFLUENCE OF MEMBRANE PHOSPHOLIPIDS ANDMEMBRANE INCORPORATION ON CYTOCHROME P-450 IA1 AND IIBI 266

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E.A. BORODIN, K.E. EGOROVANTIOXIDATIVE EFFECT OF PHOSPHOLIPID LIPOSOMES AND MICELLES,STABILIZATION AND REACTIVATION OF CYTOCHROME P-450 BYPHOSPHOLIPIDS AT LIPID PEROXIDATION IN MICROSOME MEMBRANES 270

D.R. UAVYDOV, B.V. DAROVSKY, I.P. KANAEVA. G.I. BACHMANOVA,A.I. ARCHAKOVCYTOCHROME c (Fe ) AS COMPETITIVE INHIBITOR OF NADPH-DEPENDENT REDUCTION OF CYTOCHROME P-450 LM2 IN THE SOLUBLERECONSTITUTED SYSTEM 273

D.R. DAVYDOV, S.A. GORDEEV, A.V. KARYAKIH. A.D. GERSHANOVICHKINETICS OF ELECTRON TRANSFER REACTIONS IN SIBERIAN STURGEONLIVER MICROSOMES . 278

M. ENGELKE, H. DIEHL, U. BERGMANNCONSEQUENCE OF ORGANIC SOLVENT IMPACT ON MEMBRANE FLUIDITYAND REDUCTION KINETICS OF PIG LIVER MICROSOMES 282

V.M. GUZOV, I.N. ZELKO, A.A. CHERNOGOLOV, S.A. USANOVCATALYTIC AND IMMUNOCHEMICAL FEATURES OF MITOCHONDRIAL ANDMICROSOMAL MONOOXYGENASE SYSTEMS OF THE STEROID OXIDATION 285

I. KANAEVA, I. DEDINSKII, I. SEVRUKOVA, E. SKOTSELYAS,G. KUZNETSOVA, N. SAMENKOVACOMPARATIVE STUDY OF PEROXIDASE ACTIVITIES OF MONOMERIC ANDMEMBRANE-BOUND MICROSOMAL CYTOCHROME P-450 288

A.G. LAPKO, G. SMETTAN, K. RUCKPAUL, S.A. USANOVMODIFICATION OF CYTOCHROMES P-450scc AND P-450110 WITHFLUORESCEINE ISOTHIOCYANATE AND ITS ANALOGS 291

N A. LOBANOV, C.E. HENSEY, S.A. USANOV. A. AZZIADRENODOXIN AND CYTOCHROME bs PROTECT CYTOCHROME P-45OsccFROM PHOSPHORYLATION BY PROTEIN KINASE C 294

K.N. MYASOEDOVASOME OBSERVATIONS RELATED TO OLIGOMERIC ORGANIZATION OFCYTOCHROME P-450LM2 297

Y. OHTA, S. KAWATOELECTRON TRANSFER INTERACTIONS OF CYTOCHROME P-450 INADRENOCORTICAL MITOCHONDRIA . 300

A. PERRIN, G. DEFAYE, E.M. CHAMBAZHYDROXYLASE AND LYASE ACTIVITIES OF BOVINE CYTOCHROMEP-45017a MAY BE MODULATED BY A TISSUE-SPECIFIC MEMBRANEENVIRONMENT 303

M.A. PETER, M. SCHWEIZER, D. FILIPOVIC, M.OERTLEINFLUENCE OF MONOCLONAL ANTIBODIES ON P450IIB1-CATALYZEDREACTIONS 306

T.A. RYBAKOVA, V.I. SHARAPOV, O.R. GREKEFFECT OF PHENOBARBITAL AND ZIXORYN PRETREATMENT ON THEFLUIDITY OF MICROSOMAL MEMBRANES AND MEMBRANE-BOUND ENZYMESACTIVITY AFTER LIVER TISSUE ISCHEMIA 309

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Y. SARKISSOVA, S. MARDANIANTHE INTERACTION OF ADRENODOXIN AND ADRENODOXIN REDUCTASE 312

V.I. SHARAPOV, O.R. GREKCYTOCHROME P-450-SUBSTRATE INTERACTIONS UNDER ISCHEMICDISORDERS OF LIPID COMPONENT OF RAT LIVER MICROSOMALMEMBRANES 315

I. SLEPNEVA, A. LEVINA, L. WEINERAFFINITY REVERSIBLE LABELING OF NUCLEOTIDE BINDING SITE OFNADPH-CYTOCHROME P-450 REDUCTASE 318

V. KOBLIAKOV, L. KULIKOVA, A. KOLYADATHE ROLE OF CELL ENVIRONMENT IN THE EXPRESSION OF SOMEISOFORMS OF CYTOCHROME P-450 IN HEPATOMA-27 321

0. SIBBESEN, J. LYKKESFELDT, B. KOCH, B.L. MULLERPURIFICATION OF THE HYDROXYLATING ENZYME SYSTEM INVOLVEDIN THE BIOSYNTHESIS OF THE CYANOGENIC GLUCOSIDE DHURRININ Sorghum bicolor (L) MOEMCH 324

MOLECULAR BIOLOGY. REGULATION OF GENE EXPRESSION,

PROTEIN ENGENEERING OF CYTOCHROME P-450

K. SOGAWA, H. IMATAKA, K. YASUMOTO, Y. KIKUCHI,Y. FUJII-KURIYAMA,DNA BINDING PROTEINS AND REGULATION OF P-450c GENE 327

F.J. GONZALEZHUMAN CYTOCHROMES P450: ANALYSIS THROUGH USE OF COMPLEMENTARYDNA-DIRECTED EXPRESSION 333

I. HANUKOGLUSTRUCTURES OF THE REDUCTASES OF THE MITOCHONDRIALAND MICROSOMAL CYTOCHROME P450 SYSTEMS 339

M. INGELMAN-SUNDBERG M., I. JOHANSSON, E. ELIASSONREGULATION OF ETHANOL-INDUCIBLE CYP2E1 345

K. MOROHASHI, S. HONDA, T. HASHIMOTO, T. OMURAREGULATION OF EXPRESSION OF P-450(XIB1) GENESIN ADRENAL CORTEX CELLS 352

D.W. NEBERTPROPOSED ROLE OF DRUG-METABOLIZING ENZYMES INSIGNAL TRANSDUCTION PATHWAYS 358

A.A. CHERNOGOLOV, S.A. USANOVPROBING OF STRUCTURE AND FUNCTION OF CYTOCHROMEP-450-DEPENDENT STEROID HYDROXYLASES OF ADRENALCORTEX MITOCHONDRIA 364

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Y. YABUSAKI, T. SAKAKI, M. AKIYOSHI-SHIBATA, H.OHKAWAEXPRESSION OF MAMMALIAN P450 MONOOXYGENASES INYEAST 370

V.V. BAKAYEV, L.P. ORLIKHINA, A.E. GUSHCHIN, I.I. VOTRIN,P.L. IVANOV. A.P. RYSKOVSEQUENCE ANALYSIS OF HUMAN AND RAT cDNAs HYBRIDIZING WITHMOUSE B2+cDNAx REVEALS HIGH HOMOLOGY TO CYTOCHROME P-450J 376

T.G. DUZHAK, N.I. GUTKINA, V.V. LYAKHOVICHTHE ROLE OF DIFFERENT CHEMICALS IN EXPRESSION OF CYTOCHROMEP-450IIB FAMILY IN RATS AND MICE 379

A. GOUGH, C.A.D. SMITH, N.K. SPURR, C.R. WOLFA RAPID SINGLE TUBE ASSAY FOR THE IDENTIFICATION OF ALLKNOWN MUTATANT ALLELES OF THE DEBRISOQUINE/SPARTEINEPOLYMORPHISM 382

J.E. FREEMAN, C.R. WOLFDEDUCED AMINO-ACID SEQUENCE, PREDICTED STRUCTURALFEATURES AND PROPOSED SUBSTRATE BINDING RESIDUES OFMOUSE Cyp2el 385

L. GULYAEVA, A. GRISHANOVA, V. LYAKHOVICHSTRAIN- AND TISSUE-DEPENDENT INDUCTION OF CYTOCHROME P-45OIAAND IIB SUBFAMILIES DURING DIFFERENT TYPE INDUCTION 388

N.I. GUTKINA, T.G. DUZHAKTHE EFFECTS OF COINDUCTION WITH PB-TYPE INDUCERS ON THEMONOOXYGENASE SYSTEM OF MICE 391

D. HENRIQUE, M.J. GAMA, M.C. LECHNERSTRUCTURE AND FUNCTION OF CYP2C7 GENE PROMOTER 394

P.A. KALIMANROLE OF TRYPTOPHAN OXYGENASE IN REGULATING HAEM UTILIZATIONWHEN INDUCING CYTOCHROME P-450 BY PHENOBARBITAL IN RATLIVER 397

E.YU. KRYNETSKY, I.E. KOVALEVASTUDY OF THE TRANSCRIPTION OF THE HUMAN CYTOCHROME P-450GENE (CYP2D6) BY PCR 400

E.YU. KRYNETSKY, I.E. KOVALEVA, A.M. KOPYLOV, V.N. LUZIKOV,M.A. ELDAROVEXPRESSION OF HUMAN CYTOCHROME P-45Odb BY Saccharomycescerevisiae 403

J.G. LEHOUXEFFECTS OF 4-AMINOPYRAZOLOPYRIMIDINE (4-APP) ON THE LEVELSOF STEROIDOGENIC ENZYMES nRNA IN RAT ADRENALS 406

J.C. LOPER, T.G. TURI, D.L. CRAFTMOLECULAR GENETICS OF THE LANOSTEROL 14a-DEMETHYLASESYSTEM IN Saccharomyces cerevisiae 410

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T. MARKUSHEVA, I. KUSOVA, R.N. TCHURAEVCOMPARATIVE INVESTIGATION OF GENES OF 2,4-D DEGRADATIONIN PLANT AND ANIMAL CELLS 416

L.A. NOVIKOVA, L.V. ISAYEVA, E.YU. KRYNETSKY, V.N. LUZIKOVIMPORT OF THE IN VITRO SYNTHESIZED BOVINE ADRENODOXINPRECURSOR INTO YEAST MITOCHONDRIA 419

M.F. PALIN, J.G. LEHOUX, J. SYGUSCHEXPRESSION OF RECOMBINANT BOVINE ADRENAL CYTOCHROME P450sccIN E. coii 422

T.G. PANKOVA. T.M. IGONINA, T.V. MAYER, R.I. SALGANIKCOMPARATIVE STUDY OF GENES STRUCTURE OF MALARIAL PARASITESCYTOCHROME P-450 IN RELATION TO THEIR CHLOROQUINE RESISTANCE 425

D. SANGLARD, W. SEGHEZZI, A. FIECHTERPROBING THE MEMBRANE TOPOLOGY OF YEAST CYTOCHROME P450s WITHAN IN VIVO ASSAY 428

W. SEGHEZZI, D, SANGLARD, C. MEILI, A. FIECHTERANALYSIS OF THE CYP52 GENE FAMILY IN THE YEAST Candidatropicalis 433

C.A.D. SMITH, A.C. GOUGH, J.E. MOSS. K.A. VALLIS, G. HOWARD,N.K. SPURR, C.R. WOLFMOLECULAR AND GENETIC ANALYSIS OF THE CYP2D6 LOCUS ANDCANCER SUSCEPTIBILITY 437

P. URBAN, G. TRUAN, C. CULLIN, J.-C. GAUTIER, D. POMPONSIMULATION OF LIVER DRUG METABOLISM IN YEAST COEXPRESSINGMAMMALIAN P450 AND ASSOCIATED ENZYMES 440

T. OHMACHI, I. SAGAMI, H. FUJII, H. KIKUCI. M. WATANABECLONING AND CHARACTERIZATION OF THREE FORMS OF CYTOCHROMEP-450 IN 3-METHYLCHOLANTHRENE-TREATED HAMSTER LIVERS 443

D.-A. WU, M.-C. HU, BON-CHU CHUNGEXPRESSION AND FUNCTIONAL CHARACTERIZARTION OF P450c21 INS. cerevisiae IN RELATION TO ITS DEFICIENCY 446

F. CARRIER, R.A. OWENS, L.A. NEUHOLD, D.W. NEBERT, A. PUGAACTIVATION OF MURINE Cypla-1 (CYTOCHROME P^SO) GENEREQUIRES PROTEIN PHOSPHRYLATION: POSSIBLE INVOLVEMENTOF PROTEIN KINASE C 449

L. KULIKOVA, V. KOBLIAKOVTHE ANOMALOUS HIGH EXPRESSION OF COUMARIN 7-HYDROXYLASE(CYTOCHROME P-450COH) IN MOUSE HEPATOMAS 452

A.YU. KOLYADACLONING AND SEQUENCING OF HUMAN LJVER CYTOCHROME P-450 cDNACLONES 455

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M.C. LORENCE, J. GEISSELSODER, C. CARMONA, J.Y. LU, F. WITNEY,J.A. PETERSONCHARACTERIZATION OF A P450 SYSTEM WHICH CATALYZES THEOXIDATION OF TERPINEOL 457

O.G. GREBENSHCHIKOVA, A.T. BERYOZOV, E.F. KOLESANOVA, A.I.ARCHAKOVA NEW METHOD OF 'PIN TECHNOLOGY1 EPITOPE MAPPING IN THE STUDIESOF CYTOCHROME P450 LM2 ANTIGENIC DETERMINANTS 460

BIOTECHNOLOGICAL AND ECOLOGICAL ASPECTS OF

CYTOCHROME P-450 AND RELATED HEMOPROTEINS

J. DOEHMERMAMMALIAN CELLS GENETICALLY ENGINEERED FORCYTOCHROMES P450 AND THEIR USE IN PHARMACOLOGYAND TOXICOLOGY 463

W. KLINGER, F. JAHN, F. SCHILLERMODULATION OF THE P-450 SYSTEM AS A KEY TOUNDERSTAND THE ECOLOGICAL IMPORTANCE OFAGROCHEMICALS AND METALS 469

S.V. KOTELEVTSEV, 0. HANNINENNBIOTECHNOLOGY IN ANALYSIS OF AQUATIC ECOSYSTEMS:HYDROBIONT TISSUE ISOFORMS CYTOCHROME P-450 475

V.M. SHKUMATOVHETEROLOGOUS RECONSTITUTION OF STEROID-HYDROXYLATINGSYSTEMS 481

J.J. STEGEMAN, P.J. KLOEPPER-SAMA, A.A. EKSKUSENVIRONMENTAL AND HORMONAL REGULATION OF CYTOCHROMEP450 1A FORMS IN FISH 486

S. AVETISOVA, V.V. DMITRIEVLOCALIZATION AND SUBSTRATE SPECIFITY OF CYTOCHBOME P-450MONOOXYGENASE SYSTEM OF Candida maltosa YEAST 492

M. BERDICHEVSKAYA, G. KOZYREVAMONOOXYGENASE BACTERIAL ACTIVITY IN BIOLOGICAL MONITORINGOF OIL-POLLUTED AQUATIC ENVIRONMENTS 495

A. CUANY. J.B. BERGER, F. DURST. J.P. SALAUN, R. LANGE,C. LARROQUEPARTIAL PURIFICATION OF A CYTOCHROME P-450 FROM A Drosophilamelanogaster DDT RESISTANT STRAIN 498

V.A. DADALI, A.Z. ABYSHEV, V.G. MAKAROV, G.I. NEZHINSKAYA.Y.G. CHEKALIN, K.CH. MELIKOVMONOOXYGENASE AND IMMUNE SYSTEMS ACTIVATION BY IMIDAZOLE-CONTAINING POLYMEES 501

rvii

V. DEMAKOV, A. ERYOMINA, A. GRISHANOVAPOLLUTION COMPLEX EFFECT ON THE FUNCTIONAL STATE OFCYTOCHROME P-450-DEPHJDENT MONOOXYGENASE SYSTEM 504

V.A. DOROVSKIKH, E.V. ZRAZHEVSKAYA, G.K. DOROSHENKO,G.P. BORODINA, E.A. BORODININCREASE IN LIPID PEROXIDATION IN RAT LIVER MICROSOMES UNDERPROLONGED COLD STRESS AND ITS PREVENTION BY MALONIC ACIDDERIVATIVES 507

P. FERNANDE2-SALGUER0, A.M. BUNCH, C. GUTIERREZ-MERINOKINETIC CHARACTERIZATION OF MICROSOMAL CYTOCHROME P-450FROM PHENOBARBITAL TREATED RAT IN HOLLOW FIBER BIOREACTORS 510

S.YU. FUCHS, V.S. SHPIGELMAN, G.A. BELITSKYDRUG METABOLIZING ENZYMES IN DROSOPHILA S1MULANS 513

T. FUJII, T. OGAWA, H. FUKUDAINDUCTION OF CYTOCHROME P-450rm IN Bhodotorula mimta BYL-PHENYLALANINE 516

YU.V. GALANOVA, A.I. ARCHAKOVCONJUGATION OF BLEOMYCIN WITH CONCANAVALIN A, ANTIERYTHRO-CYTIC IgG AND Fab-FRAGMENT ENHANCES ITS HEMOLYTIC ACTIVITY 519

S.N. GILEVICH, O.L. GURYEVDIRECT ELECTROCHEMICAL REDUCTION OF ADRENODOXIN AND CERTAINHEMOPROTEINS PROMOTED BY METHYL VIOLOGEN 522

A.YU. GRISHANOVA, V.V. LYAKHOVICHUSING OF ANTIBODIES TO CYTOCHROMES P-450 LIBRARY IN XENOBIOTICMETABOLISM STUDIES 525

G.-R. JAENIG, D. PFEIL, M. MULLER-FROHNE, H. RIEMER,M. HENNING, K. RUCKPAULA CYTOCHROME P-450 DEPENDENT FUNGAL STEROID 11/3-HYDROXYLASE:PURIFICATION AND CHARACTERIZATION 528

Y. JOUNAIDI, F. PERIN, F. ZAJDELA, L. ELM'HAMADI,C. LARROQUE, R. LANGECYTOCHROME P-450TU, A NEW FEMALE SPECIFIC MARKER ENZYME INMOUSE HEPATOMAS 531

H. KARIMOV, Z. HAKIMOV, F. INOIATOVA, A. RAHMANOV,A. KARBANOVITCHIMPORTANCE OF MICROSOME AND MITOCHONDRIA HEMOPROTEINSIN INJURY OF THE LIVER WITH ALKALOIDS OF HELIOTROPIUM L. 534

TS. KARTOZIA, I. ERMAK, T. EGNATASHVILI, S. AIRAPETOVATHE INFLUENCE OF DIFFERENT XENOBIOTICS ON THE CYTOCHROMEP-450 SYSTEM IN LIVER AND ADRENALS DURING EXPERIMEINTALSENSITIZATION 537

I.G. KESOVA. K.S. SEDOVA, I.B. TSYRLOVEVALUATION OF INDUCTIVE AND MUTAGENIC EFFECTS OFBENZO(A)PYRENE IN BLOOD LYMPHOCYTES UNDER EXPOSURE TO PAH 540

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P.M. KHAILOV, I.G. SIVOV, N.V. ADRIANOVBACTERICIDAL AND MUTAGENIC EFFECT OF HYPOCHLORITEAND ACTIVE OXYGEN FORMS GENERATED BY MYELOPEROXIDASEAND SOLUBLE RECONSTITUTED MONOOXYGENASE SYSTEM 543

G.A. XHATISSASHVILI, M.SH. GORDEZIANIXENOBIOTIC HYDROXYLATION BY PLANT CYTOCHROME P-450 546

P. KOPPONEN, R. TORRONEN. J. TARHANEN. J. RUUSKANEN,S. KARENLAMPICYTOCHROME P45OIA1 INDUCTION IN MOUSE HEPATQMA CELL AS ANINDICATOR OF POLYCYCLIC AROMATIC COMPOUNDS IN FLY ASH 549

N.E. KOZLOVSKAYA, A.YU. GRISHANOVA, V.M. MISHIN, .V.V. LYAKHOVICHEFFECTS OF ORGANIC SOLVENTS ON RAT LIVER NONOOXYGENASESYSTEM 552

V. KURCHENKO, I. PRONSKAYA, A. PIKULEVINDUCTION OF CYTOCHROME P-450 MOLECULAR FORMS BYAM1NOBIPHENYLS 555

N.V. LASHNEVA, G.V. CHICHILANOVA, Z.F. SINITSKAYA,V.A. TUTELYANTHE EFFECT OF TRANS-ANETHOLE AND POLYCHLORINATED BIPHENYLSCOADMINISTRATION ON THE ACTIVITY OF RAT LIVER MONOOXYGENASESYSTEM 558

V.S. LI. G.I. BACHMANOVA, A.A. POPOV, J.-CH. HAGER,R.J.H. NIEMANN, R.-J. GUNDERMANNELABORATION AND TESTING OF PHOSPHOLIPID PREPARATIONSFOR THE REPARATION OF LIVER CELL MEMBRANES INJUREDBY MEMBRANOTOXIC AGENTS 561

G. MAKHAEVA, V. MALYGIN, D. KHASKINROLE OF CYTOCHROME P-450-DEPENDENT OXIDATIVEBIOTRANSFORMATION IN TOXICITY OF SOIL INSECTICIDE DICHLORON 564

I.E. MATKHANOV, A.A. ZHUKOVCONJUGATION OF BLEOMYCIN WITH INSULIN AND CALCITONINENHANCES ITS ABILITY TO DESTROY MOUSE THYMOCYTES . 567

O.R. MELNIKOV, V.YA. MOMOT. E.P. SIDORIKCYTOCHROME P-450-DEPENDENT REACTIONS AT NITROSAMINE-INDUCEDTRANSFORMATION OF HEPATOCYTES 570

G.M. MIKHAILOV, A.A. KOLCHIN. A.A. VARYKHANOV. B.N. FILATOVTHE EFFECTS OF THE MONOOXYGENASE SYSTEM INDUCERS ON THETOXICITY OF TRIORTHOCRESYLPHOSPHATE AND NATRIUMFLUOROACETATE 573

V.YA. MOMOT, O.R. MELNIKOV, E.P. SIDORIKSTATUS OF MONOOXYGENASES IN NITROSAMINE-INDUCED HEPATOMAS 577

xix

L.H. MUHAMBETOVA, S.I. DOLINSKAYA. A.A. NASONOVA,S.L. AVALIANIASSESSMENT AND PREDICTION OF TOXIC EFFECT ON THE RASIS OFSTUDYING PHASES I AND II OF XENOBIOTICS' DETOXICATION 580

K.D. MUKANOVA, E.B. MAIZEL, A.M. GAZALIEV, S.N. BALITSKIJ,O.A. NURKENOV, B.I. TULEUOV, A.E. KHOVANSKIKHPOLYFUNCTIONAL INHIBITORS OF CYTOCHROME P-450 SYSTEM WITHSIMULTANEOUS INHIBITING EFFECT ON GLUTATHIDNE-S-TRANSFERASEAND CHOLINE ESTERASES 583

D. MULLER, TH. GROH, F.K. SPLINTER, W. KLINGERINFLUENCE OF INDUCERS OF CYTOCHROME P-450 ON DEALKYLATION OF7-ALKOXYCOUMARINS 586

S.V. NEDELKINA, L.A. VICHREVA, T.A. PUDOVA, N.N. GODOVIKOV,R.I. SALGANIKTHE ROLE OF CYTOCHROME P-450 IN METABOLISM OF SUBSTITUTEDS-ETHYNYL THIOPHOSPHATES 590

T.P. NOVOZHEEVA, A.S. SARATIKOV, R.R. AKHMEDZHANOVBENZONAL AND GALONAL AS PB-TYPE INDUCERS OF LIVERMONOOXYGENASE SYSTEM 593

V.V. OBRAZTSOV, E.V. KALININA, D.G. SCHEKHTMAN.N.I. KUKUSHKIN, A.N. SAPRINTHE EFFECTS OF PERFLUOROCARBONS ON ENZYMATIC DETOXICATIONSYSTEMS 597

V. OBRAZTSOV, A. SKLIFAS, E. MAEVSKII, D. SHEKHTMAN,N.I. KUKUSHKINIS THE INDUCTION OF CYTOCHROME P-450 A CAUSE OF RABBIT DEATHAFTER INJECTION OF PERFLUORODECALIN EMULSION? 601

A. PIKULEV, I. ISMAHIL, I. PRONSKAYA, V. KURCHENKOTHE FUNCTION OF HEMOPROTEINES THE FORMATION OF DNA-ADDUCTSUNDER THE PEROXIDASE OXIDATION OF A NUMBER AMINOBIPHENYLS WITHDECREASING ABILITY TO CARCINOGENESIS 604

L. PONOMAREVA, S. KOTELEVTSEVPHOSPHOLIPID AND FATTY ACID COMPOSITION OF LIPIDS IN LIVERMICROSOMES OF FRESHWATER FISH. EFFECT OF INDUCTIONOF MONOOXYGENASES WITH POLYCYCLIC AROMATIC HYDROCARBONS ANDPOLYCHLORINATED BIPHENYLS 607

A.A. POPOV, V.S. LI, V.KH. MITINA, B.A. KLYASHCHITSKII,O.YU. ABAKUMOVA, L.M. FEDOROVA. G.I. BACHMANOVAINFLUENCE OF ALBUMIN-BILIRUBIN COMPLEXES ON THE LIVERMICROSOMAL MONOOXYGENASE SYSTEM AND DNA SYNTHESISIN RATS WITH CCl^-INDUCED HEPATITIS 611

R.A. PSHENICHNOV, M.R. PSHENICHNOV, T.D. GOLUBKOVA,L.N. KOGINA, N.M. NIKITINACOMPARATIVE MONOOXYGENASE SYSTEMS ACTIVITY OF THE URALS WILDHERBAE MEDICINES 614

T.V. PYATCHANINA, E.P.SIDORIKIMPROVED ANTICARCINOGENIC AND ANTITUMOR VITAMIN A EFFICACYREGIMES BASED ON ASSESING CYTOCHROME P-450 AND ADRENODOXINEFUNCTION UNDER MAMMARY CARCINOGENESIS 617

H. REIN, K. POMMERENING, C. JUNG, 0. RISTAUPROPERTIES OP IMMOBILIZED CYTOCHROME P-450 ENTRAPPED INPOLYMER POLYELECTROLYTE CAPSULES 620

V.A. REMIZOV, A.V. KARYAKINNICKEL CATHODE AS A SOURCE OF ELECTRONS IN CYTOCHROME P-450DEPENDENT RYDROXYLATION REACTIONS 623

H. SHOUN, T. TANIMOTOCYTOCHROME P-450(NOR) OF THE DENITRIFIER FUNGUSFusarium oxysporum INVOLVED IN RESPIRATORY NITRITEREDUCTION 626

Y.I. SOKOLOV, T.B. PERSIANOVAPREPARATIVE ISOLATION OF TWO FORMS OF CYTOCHROME P-450 FROMALKANE OXIDIZING YEASTS Candida maitosa, THEIR SUBSTRATESPECIFICITY AND CHARACTERIZATION 629

I.I. STEPURO, N.V. KONOVALOVA, I.M. ARTSUKEVICHROLE OF OXYHEMOGLOBIN AND METHEMOGLOBIN IN PROTECTIONAGAINST HYDROXYL AND ORGANIC FREE RADICALS IN ERYTHROCYTES 632

L.I. SUSHKO, M.S. OMELYANCHIKCYTOCHROME P-450-DEPENDENT SYSTEM OF THE RAT LIVER UNDERTHE INFLUENCE OF INHALANT ETHANOL 635

T.V. TRUCHACHYOVA, G.V. NASONOVA, V.S. RADYUK, N.S.FROLOVA, V.M. SHKUMATOVSTUDIES ON THE INDUCTION OF MONOOXYGENASES FROM MICROORGANISMS 638

V. VAVILIN, 0. GROMOVA, A. CRISHANOVA, S. MAKAROVA,L. GULYAEVA. V.V. LYAKHOVICHTHEOPHYLLINE METABOLISM FEATURES IN LIVER MICROSOMES OFWISTAR RATS TREATED WITH AROCLOR 1254 641

U.K. IBRAGIMOV, A.I. NIKOLAEV, R.G. SULTANOV, L.Z. ASHUROVACORRECTION OF THE FUNCTIONAL STATE OF CYTOCHROME SYSTEMOF RAT LIVER AFTER BACTERIAL DAMAGE 644

W. KLINGER, D. MULLER, A. BARTH, U. KLEEBERG, M. KRETZSCMAR,F.-K. SPLINTERTHE INFLUENCE OF 'ESSENTIAL' PHOSPHOLOPIDS (EPL) ON P-450MONOOXYGENASE FUNCTION AND FORMATION OF OXYGEN RADICALS ANDHYDROGENPEROXIDE IN THE LIVER OF AGEING RATS 647

S. MAUERSBERGER, T.B. PERSIYANOVA, S.M. AVETISOVA, Y.I.SOKOLOV, E. KARGEL, R. KRAFT, W.-H. SCHUNCK. E.R.DAVIDOV, H.-G. MULLERCHARACTERIZATION OF TWO CYTOCHROME P-450 FORMS PURIFIEDFROM THE YEAST Candida maitosa 651

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R. MENZEL, U. SCHELLER, W.-H. SCHUNCK, H.-G. MULLERINDUCIBLE HIGH-LEVEL EXPRESSION OF CYTOCHROMES P-450CYP52A3 AND CYP52A4 FROM Candida maitosa INSaccharomyces cerevisiae 654

D. PANKOW, P. HOFFMANNIS THERE AN EFFECT OF 'ESSENTIAL' PHOSPHOLIPIDS ONDICHLOROMETHANE-EVOKED CARBOXYHEMOGLOBINEMIA IN RATS? 657

V. SCHELLER, W.-H. SCHUNCK, H.-G. MULLERCHARACTERIZATION OF TWO DIFFERENT ALKANE-INDUCIBLEP-450 FORMS FROM Candida maitosa BY MEANS OFHETEROLOGOUS EXPRESSION IN Saccharomyces cerevisiae 662

M. STIBOROVA, E. FREI, H.H. SCHMEISER, P. ANZENBACHERCOMPARISON OF CYTOCHROME P-450 AND PEROXIDASE-MEDIATED ACTIVATIONS OF CHEMICAL CARCINOGENS 665

H. KARIMOV, Z. KHAKIMOV, A. RAIMANOVTHE IMPORTANCE OF PHOSPHOLIPIDS SYNTHESIS STIMULATION INRECOVERY OF FUNCTIONAL ACTIVITY OF CYTOCHROME P-450 INCHRONIC LIVER INJURIES 670

APPLICATIONS OF COMPUTERS IN CYTOCHROME P-450 RESEARCH

A.I. ARCHAKOV, G.I. BACHMANOVA. M.K. SANDLER,I.YU. TUTOCHKIN, A.V. LISITSACYTOCHROME P-450 DATABASE AND ITS SCIENTIFIC APPLICATION 673

M.B. BASS, M.D. PAULSEN, R.L. ORNSTEINMOLECULAR DYNAMICS SIMULATIONS OF NORCAMPHOR-CYTOCHROME P-450cam AND MUTATIONS OF P-450canDESIGNED TO ALTER THE PRODUCT SPECIFICITY 680

0. GOTOHALIGNMENT OF PROTEIN SEQUENCES AS A TOOL FOREVOLUTIONARY AND STRUCTURAL INVESTIGATION OFCYTOCHROME P-450 • 686

G.M. MORRIS, W.G. RICHARDSMODELLING CYTOCHROME P-450 LANOSTEROL 14a-DEMETHYLASE USING SEQUENCE ANALYSIS AND THETERTIARY STRUCTURE OF CYTOCHROME P-450cam 692

D.R. NELSONP450 SEQUENCE ANALYSIS ON IBM COMPATIBLE COMPUTERS 702

J.A. PETERSON, T. OSTER, S.S.BODDUPALLIP-450BM-3: A PROMISING ENZYME FOR STRUCTURE/FUNCTION COMPARISONS TO MICROSOMAL P450s 708

A.I. ARCHAKOV, A.S. IVANOV, A.V. LISITSA, I.G. RUKAVISHNIKOVLEUCINE CLUSTERS (D3+N AS SITES OF INTERACTION OF MICROSOMALCYTOCHROMES P-450 WITH THE MEMBRANE PHOSPHOHPIDS 716

xxii

D.R. DAVYDOV, V.G. FREEDMAN, I.M. ERLIKH, E.E. KLOTZ, V.M.BLINOVPROFILE PATTERN RECOGNITION TECHNIQUE APPLIED TO LOCATE THECENTERS OF PROTEIN-PROTEIN INTERACTIONS IN THE AMINOACIDSEQUENCES OF MICROSOMAL ELECTRON CARRIERS 719

K.N. DEGTYARENKO, A.I. ARCHAKOVHIERARCHICAL CLUSTERING OF CYTOCHROMES P450 724

K.N. DEGTYARENKO, T.A. KULIKOVAMODULE STRUCTURE OF CYTOCHROMES P450 727

I. RUKAVISHNIKOV, K. DEGTYARENKO, V. UVAROV, A. KRITSKYPREDICTION OF MEMBRANE TOPOLOGY OF MICROSOMAL EPOXIDE HYDROLASEPROTEINS 730

W. SCHWARZE, H. SCHRAUBER, E. GRAU, K. RUCKPAULMEMBRANE TOPOLOGY OF MITOCHONDRIAL CYTOCHROME P-450 XIB1AS ANALYSED BY MEANS OF PREDICTION OF EPITOPES 733

G. STROBL, T. WOLFFSTRUCTURAL MODELS FOR INHIBITORS AND SUBSTRATES OF CYTOCHROMEP-450IID6 BASED ON MOLECULAR MODELING ANALYSIS 736

V . m UVAROV, K.N. DEGTYARENKO, A.I. SOTNICHENKO, E.L.VODOVOZOVA, YUL. G. MOLOTKOVSKY, L.D. BERGELSON,A.I. ARCHAKOV. A. STIER, V. KRUEGERTHE SPATIAL ORGANIZATION OF CYTOCHROME P-450LM2 IN MEMBRANE 739

AUTHOR INDEX 744