incubators - astec ivfcube instruction manual rev.5 2013.6.6 supplied by ri uk and ireland

AR-3100GC

Operation Manual

Drawer Type Incubator

Drawer Type Incubator Operation Manual Rev. 5 ASTEC CO., LTD.

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1. General Description ................................................................................................ 4

1-1 About This manual ............................................................................................ 4

1-2 Warranty and Liability........................................................................................ 4

1-3 Warnings ........................................................................................................... 4

1-4 Warning Labels ................................................................................................. 5

2. Bio Hazard............................................................................................................... 8

3. Important Notice...................................................................................................... 8

4. Copy Rights............................................................................................................. 8

5. Specifications .......................................................................................................... 9

6. Accessories............................................................................................................. 9

7. Parts Description................................................................................................... 10

8. Description of Control Panel ................................................................................. 11

9. Prior to Installation ................................................................................................ 13

9-1 Initial Cleaning and Disinfecting...................................................................... 13

9-2 Procedure........................................................................................................ 14

10. Humidity for the Incubation Chamber ................................................................. 15

10-1 Filling the Humidity Slot................................................................................. 15

11. Connecting gas ................................................................................................... 16

11-1 Connecting CO2/N2 Gas Supply.................................................................... 16

11-2 Gas Pressure................................................................................................. 16

12. Main Power ......................................................................................................... 17

13. Setting up AR-3100GC ....................................................................................... 17

14. Gas Concentration Check................................................................................... 18

15. Drawer type Incubation Chamber ....................................................................... 19

16. Automatic Calibrations ........................................................................................ 19

17. Basic Settings ..................................................................................................... 20

17-1 Temperature Setting...................................................................................... 20

17-2 Overtemp Setting .......................................................................................... 20

17-3 CO2 Setting ................................................................................................... 20

17-4 Gas Selection................................................................................................ 20

17-5 O2 Setting ...................................................................................................... 20

18. Adjustment and Calibration................................................................................. 22

18-1 Adjustment / Calibration Mode:..................................................................... 22

18-2 Lid Temperature Adjustment A-3 .................................................................. 22

18-3 CO2 ZERO Manual Calibration A-4 ............................................................... 22

18-4 CO2 SPAN Adjustment A-5 ........................................................................... 22

18-5 O2 ZERO Adjustment A-6.............................................................................. 23

18-6 O2 ZERO Manual Calibration A-7.................................................................. 23

Index of Contexts


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18-7 Flow Chart of Adjustment and setting ......................................................... 24

19. Chamber Temperature Calibration...................................................................... 25

20. Alarms ................................................................................................................. 26

20-1 CO2 Alarm...................................................................................................... 26

20-2 Overtemp Alarm ............................................................................................ 26

20-3 CO2 Alarm...................................................................................................... 26

20-4 O2 Alarm ........................................................................................................ 26

21. Cleaning and Disinfectant ................................................................................... 27

22. Wiring Diagram...................................................................................................... 28

23. Maintenance .......................................................................................................... 30

Descriptions of primary components ........................................................................... 31

Descriptions of Gas Sensors and Tubing.................................................................... 33

Descriptions of Gas Control Box ................................................................................. 34

Descriptions of Drawers............................................................................................... 35

Descriptions of Gas supply unit ................................................................................... 36

Descriptions of Gas supply Flow ................................................................................. 37

23. Warranty Information........................................................................................... 38


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1. General Description

1-1 About This manual

1-2 Warranty and Liability

We warrant the product you have purchased for one calendar year after the date of purchase. In case

of any malfunctions caused by the manufacturer during this period of time, ASTEC will be responsible for

repair or replacement of failed parts or even the instrument itself as circumstance allows for free of

charge.

However, this warranty excludes the following conditions.

Any damages due to fire, earthquake, thunderstorm, and other catastrophic events and pollution and

abnormal electrical supply.

Any damages due to unduly repair, adjustment, calibration and modification.

Any damages due to unduly usage or mishandling.

Any damages caused by moving, dropping, transportation of the instrument.

Replacement and repair of expendables and consumables.

For service, please contact our agent that you have purchased the instrument from.

1-3 Warnings

Connect the instrument to a line plug according to local or county standards and to the specifications.

Do not use the instrument for different purposes from those shown by the manufacturer. (See Chapter 1

for General Descriptions)

Do not switch on the instrument without having waited at least 20 seconds after switching off.

Do not open the instrument before reading the servicing specific conditions described in the manual.

Follow instruction contained in the manual in case of changing any parts

To move the instrument, at least two persons are required. We suggest to contact the distributor or

directly to the manufacturer for servicing.

Thank you for purchasing ASTEC Drawer type incubator AR-3100GC. The following

instructions are for your own protection and safety use. Please read this manual carefully

before start using the instrument. Failure to observe these precautions can result in property

damage or personal injuries. Make sure to keep this manual handy so that you can read this

whenever you may need it.

Warranty is Valid only if the installation is done by people trained, in conformity

to instructions provided in this manual.


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1-4 Warning Labels

AR-3100GC has an intrinsic danger due to the structure designed for the purpose. For the safe use of

the instrument, the following mark is labeled on the instrument. Please use this instrument under a

caution indicated on the labels.

Power inlet on the back of the incubator is labeled with the Warning sticker.

The following symbols are used in this manual.

Warning, Caution

Prohibited

Important Note

Ventilate the room in which you use carbon dioxide with the instrument. It can harm your

body without adequate air ventilation. Do not breath carbon dioxide directly.

Do not place flammable objects in the incubator, such as alcohol, benzene, ethanol or

propane gas. Especially when you use oxygen, do not use matches or lighters or

cigarettes into the laboratory.

Do not use this instrument outdoors. It could cause an electrical short or a lethal shock.

Ask the manufacturer or your sales agent for the setting up. This will prevent from the

events such as gas leaks, water leaks or any other unexpected problems caused by

mishandling.

Warning This may cause serious or lethal injury.

Caution This may cause serious injury or damage

WarningWarningWarningWarning

Electric Shock The earth terminal MUST be

earthed properly

Cautions


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The location where the instrument will be placed must be steady, firm and leveled.

Do not leave this instrument in humid or wet conditions. It could cause an electrical short.

When double-stack the incubators, use the optional brackets. The top incubator may fall

by earthquake or other unexpected cause or accidents.

Connect only to a suitable power source specifically assigned for the instrument(s)

Using the instrument outside of Japan may require a transformer. Please ask the

manufacturer or your sales agent.

The power plug should be inserted all the way into the power outlet. Make sure that the

power plug is clean and dust-free when used.

Make sure that the instrument is securely earth-grounded.

Do not earth the instrument to the gas pipe, water pipe and lighting rod. This will cause

an electrical shock.

Never use the flammable objects with the instrument.

Make sure the type of gas and appropriate gas pressure are used with the instrument. All

the gas connections with gas tube(s) must be firmly connected and tightened with

cable-tie.

Do not plug any wire or pin into gas ports, sampling port.

Toxic, pathogenic and radioactive substances should be kept away from the instrument.

During maintenance and cleaning, make sure that power off the instrument and unplug the

power code.

Do not take apart or modify the instrument without first-hand supervising.

In case of malfunctions, turn off the power switch in the back of the instrument, and then,

unplug the power cord.

When the incubator is disposed, please be concerned for environment safety.


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Pedestal, bench, desk or table where the instrument will be set up MUST be solid and

leveled even to prevent leakage of the water in the Jacket.

Using the correct power source specifically assigned for the instrument is extremely

required.

Do not unplug the power cable with soaked hands.

In case any cuts or worn-out was found on the power cable of the instrument, stop using

the cable and replace it with brand-new power cable.

Do not operate the instrument with soaked hands.

Do not put heavy objects or bottles that contain liquid.

Do not get on the instrument.

When close the inner and outer doors please hold the knob and handle in appropriate

manner to avoid pinching your hands or fingers.

Do not intentionally step on, bend or twist the power cable.

Do not lean against the instrument.

Do not lean against the door of the instrument, especially when it is open.

Use appropriate gloves when cleaning the instrument, especially for cleaning the chamber.

Do not inhale any chemicals, organic compounds, dust or moisture when cleaning.

Power off and unplug the power cable when moving the instrument to different location.

Remove the water from the water pan when moving the incubator to the different location.

In case the instrument will be unused for a long period of time, remove the water in the

water pan and dry out the chamber completely.

Do not splash the water to the outside of the instrument especially on the top of it.

The receiver of O2 sensor should be kept away from condensation.

Used O2 sensor should be properly discarded in order to avoid contamination of the natural

environment.


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2. Bio Hazard

If the product has been used with any substances that may be hazardous to human health, and it needs

to be sent back for maintenance, the product must be completely and thoroughly decontaminated before

being sent back.

3. Important Notice

(Read this section to the end prior to use for your own safety)

AR-3100GC requires separate CO2 and N2 cylinders. Premixed gas (ex. CO2: 5% O2: 5% N2: 90%) is

prohibited.

A power outlet should be solely assigned to the incubator. Use of power strip or same kind of

connections is not recommended.

Acid, benzene, soap, toothpaste are not recommended to use for cleaning.

Use of brush to any part of the incubator for cleaning is not recommended.

Do not wipe the plastic and rubber area with volatile liquid such as benzene. Use neutral detergent and

wipe off completely with water.

Do not clean the incubator by poring water (especially on top panel) and wiping it off.

Pure distilled or even cleaner water is recommended for the humidification in the chamber in order to

avoid any chance of contamination. Check the volume of remaining water at least once in 3 days.

Water for the humidification must be replaced or refilled as needed. The water should be pre-warmed

to the same temperature as the set temperature of the incubator.

Pedestal, bench, desk or table where the incubator will be set up MUST be solid and leveled.

Do not place flammable substances and volatile compounds in the incubator, such as alcohol, benzene,

ethanol, adhesive or propane gas. Especially when you use oxygen, do not use matches or lighters

and/or smoke cigarettes into the laboratory.

CO2 cylinder, which is equipped with siphon inside, cannot be used with the incubator.

Gas pressure to supply into the incubator should be maintained in the ranges between 0.03~0.05Mpa

(0.3~0.5 bar / 4.35~7.25 PSI).

In case the incubator will be kept powered off, remove the water for the humidification and dry out the

chamber completely.

The incubator MUST be properly earthed to ground.

Worn out O2 sensor should be properly discarded in order to avoid contamination of the natural

environment.

4. Copy Rights

All rights are reserved by ASTEC Co., Ltd. Revision, modification of the contents of this manual prior to

permission is strictly prohibited.


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5. Specifications

Model Drawer Type Incubator (Cat/N AR-3100GC)

Exterior Dimension W566 x D563 x H533*(mm) / W22.3 x D22.2 x H21.0*(inch)

Inner Dimension W180 x D217 x H20 (mm) / W7.1 x D8.6 x H0.8 (inch)

Drawer Chamber Capacity 775ml

4 x 4-well dish / 6 x 60mm petri dish / 12 x 35mm petri dish

Drawer Chamber Weight 2.8kg (6.2lb) / per chamber

Drawer Chamber

Number of Drawers 4

Temperature Control Digital PID control

Temperature Range 7℃ + ambient to 40℃

Temperature Accuracy (+-)0.2℃

Temperature Uniformity (+-)0.4℃

CO2 sensor Infra Red

CO2 Control Range 0~10%

CO2 Uniformity (+-)0.3%

O2 Sensor Galvanic battery sensor

O2 Control Range 4~10%

O2 Uniformity (+-)0.5%

Humidity in Chamber 90%RH

Humidity method Natural evaporation

Gas Used CO2 / N2

Gas Flow CO2 : 40ml/min / N2 : 120ml/min

Alarms User Programmable on Temperature, CO2, N2

Alarm output DC12V, 0.3V (Optional)

Safety feature Temperature cut-off (Software controlled)

Analog output Temperature, CO2, N2 (optional)

Weight 80kg / 177lb

Power 110V, 117V, 220V, 230V 50/60Hz

*Without adjustors

6. Accessories

List of standard accessories associated with AR-3100GC

Instruction Manual 1 Warranty Card 1

Plastic gas tube (3m) 1 3P adapter 1


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7. Parts Description

(1) Incubation Drawer Chamber




(5) Control Panel

(6) Analog Data Output

(7) Alarm Output

(8) Main Power

(9) Circuit Breaker

(10) AC adaptor

(11) CO2 Inlet Port

(12) N2 Inlet Port

(13) Gas Sampling Port

(14) Gas Sampling Port

(15) Chamber Lid Knob

(16) Drawer Knob

(17) Aluminum block chamber


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8. Description of Control Panel

Continues on to the next page.


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(1) Temperature Display Displays Temperature and setting value for Temperature

(2) CO2 Display Displays CO2 concentration and setting value for CO2

(3) O2 Display Displays O2 concentration and setting value for O2

(4) Heating Indicator Lights on when the chamber being heated

(5) CO2 Inject indicator Lights on when the CO2 being injected into the chamber

(6) O2 Inject indicator Lights on when the O2 being injected into the chamber

(7) Over Temp Alarm Indicator Lights on when Over temp alarm goes off with a beep

(8) CO2 Alarm Indicator Lights on when CO2 alarm goes off with a beep

(9) O2 Alarm Indicator Lights on when O2 alarm goes off with a beep

(10) Temp Set Indicator Lights on when chamber temperature being set

(11) Over Temp Set Indicator Lights on when temperature upper limit being set

(12) CO2 Set Indicator Lights on CO2 concentration upper limit being set

(13) N2/O2 Set Indicator Lights on when gas selection N2/O2 being modified

(14) O2 Set Indicator Lights on O2 concentration upper limit being set

(15) UP arrow key Used to increase value for settings and calibrations

(16) Set key Used to set value for settings and calibrations

(17) Down arrow key Used to decrease value for settings and calibrations

(18) Silence key Used to mute alarm when activated

(19) Control Panel for 2nd Drawer Same configurations as the control panel for the 1

st drawer

(20) Control Panel for 3rd Drawer Same configurations as the control panel for the 1

st drawer

(21) Control Panel for 4th Drawer Same configurations as the control panel for the 1

st drawer


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9. Prior to Installation

� The place (bench, table or stand) where the incubator will be set up MUST be steady, firm and

leveled. Make sure the surface supports the weight of the incubator in operational conditions.

� To move the incubator, two or more persons are needed.

� Please keep 10 inches or more of clearance in the back and both sides of the incubator.

� Avoid placing an incubator where cold air drifts directly to the incubator such as air conditioning or

air vent. It may cause condensation in the chamber.

� All the surface of the chamber should be cleaned and disinfected before operation.

� The incubator should be placed in a clean room environment.

When placing the drawer type incubator on the optional base stool (P/N: AR-3100STD) balance the level

of incubator adjusting the height of the foots located at each bottom corner of the incubator and secure it

to the shelf using M6 screw bolts included in the base stool package. Drawer Type incubator has

dedicated threaded holes on the bottom surface where indicated by red circles in the below diagram.

9-1 Initial Cleaning and Disinfecting

Cleaning and disinfecting the incubator before its use are critical steps. The initial process and

subsequent maintenance should be done properly and following the steps included in this manual.

The incubator should be cleaned and disinfected with substances that can be removed with proper

rinsing. Any toxic substance or its residue could be biologically toxic which potentially affecting the

culture conditions end specimens development.

Figure. Bottom Surface of AR-3100GC

Front

Back

Threaded Holes


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9-2 Procedure

Wipe all incubator surfaces (internal and external) with a 3% solution of 7X detergent in distilled water

using a damp sponge or a soft cloth (Kim wipes).

Rinse with distilled water using a damp sponge or soft cloth.

Wipe all surfaces (internal and external) with OH Peroxide solution (3%).

Rinse with distilled water using a damp sponge or soft cloth.

Dry the incubator chamber and all incubator parts with a lint-free soft cloth.

Wrap aluminum incubation block for sterilization (use steam sterilizer or oven only - DO NOT USE

ETYLHENE OXIDE METHOD for this purpose).

Once the sterilization of the incubator parts is completed and the parts are at room temperature open the

wrapping and install it.

The power outlet

Please double check for the appropriate power voltage suitable for the IVF CUBE you have purchased.

The power outlet must be individually assigned to the unit; we do not recommend multiple connections

for any circumstance.

The product must be properly grounded to the earth to avoid electric shock when short circuit.

Connect the incubator to a grounded dedicated circuit only. The power cord connector is the

main disconnect device for the incubator. Position the unit so that it can be easily disconnected.


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10. Humidity for the Incubation Chamber

10-1 Filling the Humidity Slot

Water in the humidity water slot should be distilled water and supplied in the slot with approximately

20ml of the water. Humidity water, because it is stagnant, can be common source of contamination

within the incubator. So we strongly recommend that the humidity water should be replaced once in at

least 3 days or more often, and the water slot should be autoclaved as often as possible.

It is also recommended to warm up the humidity water to about 37C before it is poured into the slot for

faster evaporation.

When the incubator is powered off more than 30 minutes, make sure to remove the water

from the chamber. When incubator is powered back up, make sure there is NO humidity in

the chamber. Humidity can harm CO2 and O2 sensor when incubator is powered off.

Humidity Water Slot

Open Space

Do not fill water in the open space to avoid clogging the CO2 supply port by the water.


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11. Connecting gas

11-1 Connecting CO2/N2 Gas Supply

The Gas port is located on the back of the incubator as shown in the figure below. Connect plastic

tubing (supplied with the incubator) and firmly tie them up with cable ties or clamps. The other end of

the tubing needs to be connected to gas regulator of gas supply tank or gas manifold.

11-2 Gas Pressure

The optimal gas supply pressure is approximately 0.03 Mpa (0.3 bar / 4.35 psi).

Excess gas pressure may cause malfunction and/or damage of the incubator.

N2 Gas Port

CO2 Gas Port


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12. Main Power

Main power switch is located in the back of the unit as shown in the figure below. Make sure that the

AC cable is plugged into the power outlet assigned individually for the incubator, then, turn on the unit.

13. Setting up AR-3100GC

Gas-Stop Mode

The Gas Stop Mode is recommended for setting up the incubator for the first time, or setting it up again

after a complete cleaning and disinfection process, or after a long-time unused period.

Gas-Stop Mode: Press and hold the SET key on the control panel for about 5 seconds.

The ON/OFF control of Gas Stop Mode is independent for each chamber.

During the Gas Stop Mode, the Temp display shows the actual temperature and “G.ST” intermittently,

and both CO2 and O2 solenoid valve operations completely stop but warm up the chamber(s) in order to

bring the temperature and humidity to the stable condition before the gas supply.

The Gas Stop Mode needs to be turned off manually. To return the chamber(s) into normal operation,

press and hold the SET key on the control panel for about 5 seconds while the chamber(s) is in Gas Stop

Mode.

Main Power Switch


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14. Gas Concentration Check

CO2 / O2 Concentration check

The CO2 and O2 gas concentration must be checked frequently using an analyzer. It is always

recommended to use an analyzer that has air-circulating function between the device and the incubator

to minimize the environmental change in the incubator during the measurement.

There are 2 sampling ports on each drawer, and either port can be used for sampling. The left

sampling port is covered by a cap. Remove the cap and connect one of the ports to the sampling port

of the gas analyzer, and connect the other port to the outlet port of the gas analyzer. Let the analyzer

read the concentration continuously until you finish the calibration. Ambient air remaining in the analyzer

may dilute the chamber concentration and the display values slightly drop from the current values. Wait

about 2 minutes for both incubator’s display and analyzer’s reading to become stable and compare the

difference in the concentration.

If the displayed CO2 and/or O2 concentration differs from the concentration measured with calibrated

analyzer, adjust the concentration on the display of the incubator to the actual concentration measured

with the analyzer. For the CO2 display adjustment, change the CO2 Span parameter to the same value

as that of actual CO2 concentration at A-4 menu. For the O2 display adjustment, calculate the

difference between the two values (actual O2 concentration - O2 display) and add this number to the

current value in A-6 menu. Refer to page 22 and 23 for more detail of adjustment menu.

Note: It is strongly recommended to use an analyzer which can circulate air between the incubator and

the device to prevent significant gas concentration change in the drawer chamber.


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Sampling Mode: <UP> key pressed for 5 seconds *Factory Use Only

During the Sampling Mode, Temperature display shows “SP” and the current temperature for 3 minutes.

Concentration check must be done during this period. After 3 minutes, the Sampling Mode

automatically cancels and goes back to normal function.

15. Drawer type Incubation Chamber

Circulation of the gas in the Chamber

Regardless of the lid of the drawer is opened or not, once the drawer is pulled out and pulled back in,

AR-3100GC starts circulating the gas in the drawer chamber

As soon as the drawer is pushed back in, it starts circulating the gas in order to mix the gas in the

incubation chamber and CO2 sensors (and tubes). This helps the sensor to detect the correct gas

concentration and much faster concentration recovery. The gas circulation continues for 1 minute, and

during this circulation, O2 display shows “CYC”. During this 1 minute period gas supply into the

chamber is stopped.

16. Automatic Calibrations

AR-3100GC performs calibrations on CO2 and O2 sensors once in every week. During the calibration

of CO2 sensor, CO2 display shows “CAL” and actual concentration alternately. When O2 sensor is

calibrated, O2 display shows “CAL” and actual O2 concentration alternately.


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17. Basic Settings

Every operation and parameter can be changed by using <SET> key on the front control panel. Using

<UP> and <Down> cursors allows changing the value for the settings or changing the parameters.

After changing the value or parameter, if the operation is left unchanged about 7 seconds, it goes back to

the normal display, which indicates the current condition of the incubator.

17-1 Temperature Setting

Press <SET> key once, the TEMP SET indicator lights on. Use <UP> and <DOWN> cursors to set the

temperature to the value you wish to set.

Press <SET> key once more, the control panel brings you to Over Temp Setting. If the control is left

unchanged for about 7 seconds, it returns to normal display.

17-2 Overtemp Setting

Press <SET> key until the Over temp set point lights on. Use <UP> and <DOWN> cursors to set the

temperature to the value you wish to set. In case the incubator reaches the temperature you set here in

the chamber, it will activate the Over Temp alarm.

For detailed descriptions for Over Temp Alarm, see the Alarms section on Page 24.

Press <SET> key once more, the control panel brings you to CO2 concentration setting. If the control is

left unchanged for about 7 seconds, it returns to normal display.

17-3 CO2 Setting

Press <SET> key until the CO2 set point lights on. Use <UP> and <DOWN> cursors to set the CO2

concentration you wish to set.

Press <SET> key once more, the control panel brings you back to the Temperature Setting.

If the control is left unchanged for about 7 seconds, it returns to normal display.

17-4 Gas Selection

Always choose N2 by Use <UP> and <DOWN> cursors

17-5 O2 Setting

Press <SET> key until the O2 set point lights on. Use <UP> and <DOWN> cursors to set the O2

concentration you wish to set.

If the control is left unchanged for about 7 seconds, it returns to normal display.


21


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18. Adjustment and Calibration

Adjustment / Calibration Mode is utilized for modifications to Temperature and CO2 concentrations by

changing factory pre-set parameters as needed. The modifications should be made accordingly to

adjust to the environment in which the incubator is used.

Some of the pre-set parameters should be modified only by a qualified service technician, as they are

critical to overall performance of the incubator. These parameters are indicated here below in this

section.

By pressing <SET> key for about 0.5 sec with <UP> and <DOWN> keys pressed and held, the

Adjustment / Calibration Mode will be activated.

18-1 Adjustment / Calibration Mode:

<UP> + <DOWN> Pressed and Hold + SET pressed for 0.5 sec

Temperature Adjustment (In-chamber temperature) A-1

When Temperature display shows ST1 in Adjustment / Calibration Mode, press SET key to go to

section A-1. You will see a value in this section. Increasing or decreasing the value here allows

you to adjust the actual temperature in the chamber adjusted to the actual temperature.

18-2 Lid Temperature Adjustment A-3

When Temperature display shows ST1 in Adjustment / Calibration Mode, press SET key until you

see section A-3, Increasing or decreasing the value here allows you to adjust the temperature.

For example, when you need to increase the displayed temperature by 0.2 C, decrease the value

shown by 0.2 using <UP> and <DOWN> keys.

18-3 CO2 ZERO Manual Calibration A-4

This function allows the incubator to carry out manual ZERO Calibration CO2 concentration. As a

default, A-4 shows OFF on the display. Change this to ON with <UP> and <DOWN> cursors and

wait for 10 second until the incubator starts manual calibration.

18-4 CO2 SPAN Adjustment A-5

When measured CO2 concentration is different from the CO2 display concentration and it needs to

be adjusted, change the value on A-5 to actual CO2 concentration by using <UP> and <DOWN>

cursors. It will allow the incubator to automatically adjust the value to the measured value.


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18-5 O2 ZERO Adjustment A-6

When measured O2 concentration is different from the O2 display concentration and it needs to be

adjusted, calculate the difference between these two values (A) and change the current value in A-6

by the same amount using <UP> and <DOWN> cursors. After changing the A-6 value, go back to

the normal display and confirm that O2 display shows the same value as that of the measured O2

concentration.

(A) = (Measured O2 Concentration) – (O2 Display Concentration)

18-6 O2 SPAN Manual Calibration A-7

This function allows the incubator to carry out manual ZERO Calibration for O2 concentration. As a

default, A-7 shows OFF on the display. Change this to ON with <UP> and <DOWN> cursors and

wait for 10 second until the incubator starts manual calibration.

Make sure to use a calibrated thermistor when temperature in chamber is re-calibrated.

Do not change the value on A-2 and b1~Gb as these setting are only used by the

manufacturer.


24

18-7 Flow Chart of Adjustment and setting


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19. Chamber Temperature Calibration

1. Prepare a calibrated thermometer and attach the sensor at the center of the dish plate surface where

indicated as “CAL Point” (Figure A-1). An electronic thermometer with thin lead is recommended.

2. After setting a thermometer in the chamber, close the lid and wait at least 1 hour. Once lid is opened,

it should be left running at least another 1 hour until the temperature is stabilized completely.

3. If the temperature on the thermometer indicates different value from the Temp display, go to "ST1"

and "A-1" for adjustment. Increase the value on "A-1" brings the temperature down, and decrease

the value brings the temperature up. For example, when set temp is 37.0 C and the actual temp is

36.6, you need to set the control temp up by 0.4. In this case, decrease the value by 0.4 in order to

bring up the actual temperature by 0.4.

Lid Temperature Adjustment

When Temperature display shows ST1 in Adjustment / Calibration Mode, press SET key until you see

section A-3, Increasing or decreasing the value here allows you to adjust the temperature.

For example, when you need to increase the displayed temperature by 0.2 C, decrease the value shown

by 0.2 using <UP> and <DOWN> keys.

Figure. A-1

CAL Point

In case you need to carry out the Calibration more than once, make sure that you have

at least 1 hour between Calibrations for the chamber temperature to stabilize.


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20. Alarms

20-1 CO2 Alarm

Press <SET> key in Function suspending mode until Temperature display shows ALA. Then, CO2

display shows ON or OFF. Use <UP> and <DOWN> key to change the setting. This function only

suspends CO2 alarm.

20-2 Overtemp Alarm

OVERTEMP. Alarm activates when the temperature inside the chamber reaches the temperature set on

OVERTEMP setting. The alarm triggers a beeping sound that can be shut off by pressing the

<SILENCE> key. However, OVER TEMP setpoint indicator keeps blinking until the temperature in the

chamber actually decreases. While the alarm is being activated, incubator partially shuts down the

power to all chamber heaters for safety reasons.

20-3 CO2 Alarm

CO2 Alarm activates when CO2 concentration is off ±1.0% or more from the concentration that is set on

CO2 concentration setting for 15 consecutive minutes. The alarm triggers a beeping sound and CO2

Alarm indicator blinks. By pressing <SILENCE> key, beeping shuts down and the CO2 indicator turns

off.

20-4 O2 Alarm

O2 Alarm activates when O2 concentration is off ±1.0% or more from the concentration that is set on O2

concentration setting for 15 consecutive minutes. The alarm triggers a beeping sound and O2 Alarm

indicator blinks. By pressing <SILENCE> key, beeping shuts down and the O2 indicator turns off.


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21. Cleaning and Disinfectant

Disinfecting the incubator interior

The incubator should be cleaned and disinfected with substances that can be removed with proper

rinsing. Any toxic substance or its residue could be biologically toxic which potentially affecting the

culture conditions end specimens development. The instructions here included have demonstrated to

clean and disinfect the incubator and its components allowing proper culture conditions as demonstrated

by validation testing and Mouse Embryo Assay (MEA).

Preparation

It is recommended to use gloves, safety goggles as well as a protective coat. Make sure the incubator is

unplugged.

Procedure

1. Turn the incubator off and unplug the power cord from the outlet wall. Shut off the CO2 supply from

the gas cylinder or gas manifold.

2. Open the lid of the incubation drawers and remove water from the slot.

3. Remove the aluminum incubation block out of the drawers.

4. Wipe all incubation drawer surfaces (internal), lids with a 3% solution of 7X detergent in distilled

water using a damp sponge or a soft cloth (Kim wipes).

5. Rinse with distilled water using a damp sponge or soft cloth.

6. Wipe all incubator surfaces (internal), lids with OH Peroxide solution (3%).

7. Rinse with distilled water using a damp sponge or soft cloth.

8. Dry the incubator chamber out with a lint-free soft cloth.

9. Wrap the aluminum incubation blocks for sterilization (use steam sterilizer or oven only - DO NOT

USE ETYLHENE OXIDE METHOD for this purpose).

10. Once the sterilization of the aluminum incubation block is completed and the parts are at room

temperature open the wrapping and install it back in the drawers.

11. Plug in the incubator and turn the incubator on. Pour humidity-water to the water slot with water at

room temperature (or higher temperature) for humidification of the drawers.

12. Initiate a Mode. The incubator will start heating until it reaches the set temperature. The chamber will

increase its humidity gradually. Open the CO2 flow from the gas cylinder or manifold. The incubator

will not inject CO2 until the Set Up Mode cycle is completed.

13. After Set Up Mode is completed (12 hours) the incubator will initiate CO2 injection into the chamber

to a pre set 5%. Adjust the incubator to the desired setting if different than the factory setting (5%

CO2 and 37C).


28

22. Wiring Diagram

110V, 115V 50/60Hz


29

220 – 240V 50/60Hz


30

23. Maintenance

Panels on the top, backside and right side can be removed as show below for maintenance service.

Top panel Screws

Side panel Screws

Screws

Back panel


31

Descriptions of primary components

CPU Board Locations

Main Parts on the CPU Platform

*Note: Analog output is an optional feature.

CPU for the 1st Chamber

CPU for the 2nd Chamber

CPU for the 3rd Chamber

CPU for the 4th Chamber

Right Side

Platform

CPU Board

Step Down Transformer

Air Filter (For air intake)

N2 Valve

CO2 Valve

CO2 Sensor Board

*Analog Output Board

N2 Flow Volume Control

CO2 Flow Volume Control


32

Right Side

Top Side

In-Line Filter Step Down Transformer (AC200 – 240V only)

Switching Power Supply


33

Descriptions of Gas Sensors and Tubing

Heat Protected Box Replacement

� Remove 2 screws and gently pull out heat-protected box a few centimeters.

� Unplug the connectors and completely remove heat-protected box.

Main Parts in the Heat-Protected Box

Heat Protected Box

Connector to Heating Element (also one on the other side)

Back Side

Screw (also one on the other side)

IR CO2 Sensor O2 Sensor

Gas Control Box

Calibration Valve

Circulation Pump

Circulation (IN)/ N2 Supply Port

Circulation (OUT)

CO2 Supply Port


34

Descriptions of Gas Control Box

CO2 Sensor Replacement

� Remove gas control box from the incubator by loosening screws on each side.

� Unscrew 4 screws and pull out the CO2 sensor when you need to replace it.

O2 Sensor Replacement

� Remove gas control box from the incubator by loosening screws on each side.

� Pull out the O2 sensor from the gas control box. The O2 sensor can be replaced without removing

sensor holder and O-rings.

� Place a new O2 sensor to the original position. Make sure that sensor completely fits to the sensor

slot. Gas leakage may occur if the sensor is not properly positioned.

Screws (one on each corner)

IR CO2 Sensor

Rubber Gasket

O2 Sensor

O2 Sensor Holder

O Rings

Gas Control Box

Gas Control Box Bracket

Screw


35

Descriptions of Drawers

� Remove 4 screws located on the heating surface and remove the aluminum plate. Temperature sensor, heating element and magnet switch for incubation box can be replaced.

� Simply remove the screws that hold the magnet switch for drawer and incubation box when these switches need to be replaced.

� Use a hexagonal wrench to remove the hinge.

Heating Surface Temperature Sensor

Screws

(Beneath the heating surface)

Cables

Toothed Wheel

Toothed Wheel

Magnet Switch (Drawer)

Magnet Switch (Incubation Box)


36

Descriptions of Gas supply unit

Disconnect all the tubes to the Gas supply ports in the back of the unit (please see Descriptions of Gas

Sensors and Tubing), and remove the 2 screws holding the gas supply unit. Then pull out the whole

gas supply unit.

CO2 Port Circulation (OUT)

Circulation (IN) / N2 Port

Knobbed Nut

Silicon Gasket

Screws


37

Descriptions of Gas supply Flow

Check Valves

N2 Port Air Filter Flow Volume N2 Valve

IR CO2 Sensor

O2 Sensor

Gas Control Box

Three-way Valve

Circulation (IN)/ N2 Supply Port

Circulation (OUT)

CO2 Supply Port

Circulation Pump

CO2 Port Air Filter Flow Volume CO2 Valve

To the other Drawers

To the other Drawers

Air Filter Pump Ambient Air


38

23. Warranty Information

We warrant the product you have purchased for two years after the date of purchase. In case of any

malfunctions caused by the manufacturer during this period of time, ASTEC will be responsible for repair

or replacement of failed parts or even the instrument itself as circumstance allows for free of charge.

However, this warranty excludes the following conditions.

Any damages due to fire, earthquake, thunderstorm, and other catastrophic events and pollution and

abnormal electrical supply.

Any damages due to unduly repair, adjustment, calibration and modification.

Any damages due to mishandling.

Any damages caused by moving, dropping, transportation of the instrument.

Replacement and repair of expendables and consumables.

For service, please contact our agent that you have purchased the instrument from.

Warranty is Valid only if the installation is done by people trained, in conformity to

instructions provided in this manual.


39

Factory Settings

1st Drawer

A-1

A-3

b-2

ASTEC CO., Ltd

4-6-15, Minamizato, Shime, Kasuya, Japan 811-2207

Tel: +81-92-935-5585 Fax: +81-92-936-6613

Email: [email protected]

Website: http://www.astec-bio.com/global/

2nd Drawer

A-1

A-3

b-2

3rd Drawer

A-1

A-3

b-2

4th Drawer

A-1

A-3

b-2

incubators - astec ivfcube instruction manual rev.5 2013.6.6 supplied by ri uk and ireland

Education

manual calibration

co2 setting

temperature setting

adjustment calibration

overtemp setting

gc operation manual

co2 span adjustment

lid temperature adjustment