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Indiana University P575 1 DNA Diffraction

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Page 1: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Indiana University P575 1

DNA Diffraction

Page 2: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

X-ray diffraction and biomolecular structure

Indiana University P575 2

DNA x-ray diffraction Diffraction by tungsten coil

Page 3: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Light is an electromagnetic wave:

Indiana University P575 3

Diffraction Refraction

Hygens’ Principle:

Every point on a wavefront can be considered a secondary point source. Wavelets emitted by all points on the wavefront interfere with each other to produce the final traveling wave.

Page 4: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Diffraction of plane wave at large slit

Indiana University P575 4

Page 5: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Diffraction of plane wave at small slit

Indiana University P575 5

Page 6: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Indiana University P575 6

MIT Ripple Tank Demo

Water Waves

Page 7: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Superposition

Indiana University P575 7

Superposition of sinusoidal waves

Page 8: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Single slit diffraction

Indiana University P575 8

Alternations regions of high/low intensity:

Constructive/Destructive Interference

path length difference,

nconstructive

n destructive

(n = 0, 1, 2, …)

Page 9: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Double slit interference

Indiana University P575 9

d sin = m constructive interference

d sin = (m+1/2) destructive interference

(m=0,1,2,….)

Page 10: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Diffraction grating

Indiana University P575 10

Page 11: Indiana University P5751 DNA Diffraction. X-ray diffraction and biomolecular structure Indiana University P5752 DNA x-ray diffractionDiffraction by tungsten

Convolution, pictorially

Indiana University P575 11


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