1 Info-CRIPA January 2014 I No 7

Swine and Poultry

Infectious Diseases

Research Center

Info-CRIPA January 2014 I No 7

Annual report for the activity of the Swine and Poultry Infectious Diseases Research Center http://www.crip.umontreal.ca/en/home

Activity for the November 2012 to December 2013 period

2 Info-CRIPA January 2014 I No 7

A Word from the Director

As you flip through the pages of the annual 2012-2013 report, you will

see the results of another great year. We have accomplished a major goal

for our network! Last May, we successfully renewed our FRQNT grant,

which will allow us to continue pursuing our mission.

We are proud to now call ourselves the CRIPA. The group now includes

experts in poultry research and in the fields of animal nutrition, aerobiolo-

gy, epidemiology, pathogenesis of infection, and clinicians in poultry

health. Our research programs have been strengthened by the addition of

new research fields, including virology, mixed infections, treatment stra-

tegies, alternatives to antibiotics and the judicious use of antibiotics,

and veterinary public health. The cooperation of several strong

research programs across Quebec allows us to share our expertise in porcine and poultry infectious

diseases more effectively and to continue finding new solutions.

Our group hosted several important networking activities. The 6th CRIP symposium was held in con-

junction with the 80th ACFAS conference, which focused on animal microbiota on May 8 and 9 2013.

This symposium achieved its objective of creating a dynamic environment that promoted the ex-

change of ideas and research collaborations. This also provided our students with the opportunity to

present most of the research data of our group members.

Following the symposium, the 3rd edition of the Café-CRIPA took place on May 29th 2013. Jointly

organized by the FMV diagnostic services, the Café-CRIPA took place during the annual CAHLN

meeting focusing on the PRRS virus and was a great success.

An important part of our mission is the training of highly qualified students and personnel. Several

workshops and conferences complemented our yearly program. A student committee was formed

this summer and has already met three times to improve and direct our future activities.

Following an internal survey of our members, it was recognized that we need to improve the dif-

fusion of our results and increase the visibility of the expertise of our group. To achieve this, a

knowledge transfer committee was created and a designated knowledge transfer resource per-

son was hired in the fall of 2013.

I invite everyone to discover our achievements described within this 7th edition of the Info-

CRIPA. I highlighted individual contributions and would like to thank each and every one of you

for having participated in this group adventure.

Enjoy the read,

Josée Harel

Director

3 Info-CRIPA January 2014 I No 7

We Welcome our New Members 2012-2013

Professor Brian Geoffrey Talbot has been a member of the CRIP since its

creation in 2006 until 2012. He was a longtime collaborator with the

GREMIP, he was also a member of the Canadian Research Network on

Bacterial Pathogens of Swine (CRNBPS - 2000-2005), the Canadian Swine

Infectious Diseases Network (SidNet - 2005-2007) and the Regroupe-

ment Valorisation Recherche Québec (veterinary vaccines - 2002-2006).

He collaborated with Drs. François Malouin, Mario Jacques and Marie

Archambault. Dr. Talbot began his career in 1986 at the University of Sherbrooke in the

biology department. His research interests focused on the development of a vaccine to treat

Staphylococcus aureus, a causative agent of bovine mastitis, molecular DNA transporters and

the immune response to DNA vaccines. Known for his innovative teaching approaches,

Dr. Talbot’s career spanned 26 years at the University of Sherbrooke.

Thank you to Dr. Talbot for his major contribution and active participation.

Research interests: Poultry in

all its forms: feathers, carcass,

and eggs; risk factors for Sal-

monella and Campylobacter

contamination and alternate

strategies to antibiotic use.

Martine Boulianne

U Montréal

Research inte-

rests: Probio-

tics and bac-

terial probio-

tics in disease

prevention

and resistance

to enteric

infections;

rapid detection of pathogenic

microflora (gene probes, PCR,

RT-PCR, NASBA); in vitro

simulation of the human diges-

tive system.

Ismail Fliss

U Laval Frédéric Guay

U Laval

Research interests: Mana-

gement and nutrition of

swine, role of vitamins

and minerals in nutrition;

organic pork production,

mycotoxins and swine

nutrition.

Research interests:

microbial virulence

strategies that affect

the host immune res-

ponse; mechanisms

used by pathogenic

bacteria to initiate in-

testinal infections,

vaccine development,

and prebiotics and probiotics as alterna-

tives to antibiotics.

Xin Zhao

U McGill

Research interests: Single cell

adaptation, molecular ecology

of procaryots, bacterial molec-

ular genetics, collaborates

with S. Charette and D.

Grenier.

Michel Frenette

U Laval

Research inte-

rests: Pharma-

cological toxi-

cology, phar-

macogenetics,

and exotic ani-

mal pathology

– PRRS virus

and Streptococcus suis project.

Marie-Odile Benoit-Biancamano

U Montréal

Retiring

Younès Chorfi

U Montréal

Research interests: Effect of

mycotoxins on animal health

and immune system perfor-

mance, on susceptibility to

infections; improvement of

animal health by targeted

nutrition and feeding.

Maryse Boucher

Cégep de Saint-Hyacinthe

Research inte-

rests: collabo-

ration with Dr.

J. Daniel Du-

breuil focused

on E. coli ente-

rotoxins.

4 Info-CRIPA January 2014 I No 7

Administrative News

CRIPA Student Committee

Following a consultation with the CRIPA student members,

the student committee was formed at the beginning of the

summer. Alexandre Thibodeau represents the post-doctoral

fellows, Philippe Vogeleer represents the PhD candidates,

and Martine Audet represents the Master’s students.

The mandate of this committee is to represent students, propose and recommend new activities

to the CRIPA executive committee, and organize specific activities, such as training activities

during the annual CRIPA symposium.

STRATEGIC GROUP FRQNT: The CRIPA was renewed for 6 more years!

In an uncertain funding atmosphere, the Swine Infectious Diseases Research Centre (CRIP) renewed its

funding from the FRQNT. This funding totals over 2.8 million dollars for 6 years and now includes the

poultry industry, thus the new acronym CRIPA (the A for avian). The CRIPA includes over 40 researchers

from different universities, government institutions and industrial partners, and the CEGEP of

Saint-Hyacinthe.

The CRIPA mission is to contribute to the treatment, management and eradication of infectious diseases

of economic importance to the porcine and poultry industries that can also have an impact on public

health. Therefore, the CRIPA participates in the Quebec research mission.

New CRIPA executive Committee

The CRIPA executive committee was formed this summer. The committee is composed of the director,

the assistant director, the head of each research sector, the vice-dean (Research) of faculty, repre-

sentatives from the pork, poultry (including eggs sector) and pharmaceutics industries and the CRIPA

coordinator. In addition, a student representative is present during the committee meetings. The new

committee members include:

Josée Harel, Carl A. Gagnon, Denis Archambault, Charles M. Dozois, Martin Chénier,

Jean-Pierre Vaillancourt, John M. Fairbrother, Ann Letellier, Pierre Falardeau, Danielle

Pettigrew, Angèle Hudon-Tanguay, Julie Dufresne, Martine Labonté, Isabelle Sénéchal, Jacques

Lussier, Josée Daigneault and Alexandre Thibodeau.

The mandate of this committee is to meet annually to:

Decide the direction of the CRIPA for the upcoming year;

Approve the budget;

Debate and vote on CRIPA rules;

Approve new members.

CRIPA Knowledge Transfer Committee

This new committee focuses and develops policies and knowledge transfer chan-

nels for the CRIPA. It has developed a strategy to share information and remains

in contact with members to showcase their research achievements. This

committee includes CRIPA members Drs. Marcelo Gottschalk and Martine

Boulianne, a representative from porcine sector, Dr. Martine Denicourt, and

a representative from the poultry sector, Mr. Denis Frenette from the FPOCQ,

as well as the CRIPA coordinator Cécile Crost and the knowledge transfer

agent Hélène Poirier, who joined the network in September 2013.

The mandate of this committee is to meet once or twice yearly to increase the visibility of

our results with the end-users.

Hélène Poirier

5 Info-CRIPA January 2014 I No 7

News from our members

Sylvain Quessy and Ann Letellier, Université de Montréal

During the “Gala d’excellence de la Fondation Estrienne en environnement” held on October 23 2012,

Laboratoire M2 (Drs. Ann Letellier and Sylvain Quessy) won the top prize in the “Innovation en dé-

veloppement durable” category.

For their project aimed at improving food safety and quality,

funded by the Canadian International Development Agency

(CIDA), Sylvain Quessy and Ann Letellier completed their

mission in Vietnam in December 2013. This project will end in

March 2014 with the submission of the final report.

The “Label vert” certification of VietGAP was awarded to 14 agri-

cultural businesses of South Vietnam for their good agricultural

practices. These businesses now have access to international markets such as Europe, Japan and the Uni-

ted States of America. This is the result of 7 years of cooperation between Canada via the Research Chair

in Meat Safety (RCMS) and Vietnam.

Dr. Letellier was also on the TV show “L’épicerie” televised on Wednesday March 27 on Radio-

Canada. She commented on the fact that the USA added six new E. coli serotypes other than O157 to

the list of pathogens detected in beef. To see the TV show, go to http://www.tou.tv/l-epicerie/S2012E26,

Dr. Letellier’s contribution is at the 8 min mark.

She also received the 2013 “Prix d’excellence Vétoquinol pour la recherche” which is awarded to a

faculty member that demonstrated outstanding contribution to the training of graduate students in

veterinary sciences.

In collaboration with the Éleveurs de porc du Québec, Dr Letellier completed two projects for the

CDAQ (Conseil pour le Développement de l’Agriculture du Québec). These projects were entitled

“Comparaison des médicaments vétérinaires homologués au Canada en comparaison aux princi-

paux pays producteurs de porcs et importateurs de viande porcine canadienne” and “Enquête sur

l’utilisation des antibiotiques en production porcine au Québec dans le groupe naissance-

finition”. The research performed in both projects will have a significant impact on the stakeholder

in the swine industry.

Josée Harel, Université de Montréal

On October 21 2013, Dr. Josée Harel introduced the Swine and Poultry Infectious Disease

Research Centre (CRIPA) to over twenty representatives from French agrifood businesses and

research groups. These representatives visited the Faculté de Médecine Vétérinaire and its

researchers.

Under the umbrella of the Valorial and Vitagora competitiveness clusters, the members of these

organisations hope to discuss the techniques and processes used in certain fields such as anti-

biotic resistance, genomics, bacteriophages, and public health.

Martine Denicourt, Université de Montréal

Since the 8th of November 2013, a new executive committee directs the “AVIA”. The new

president Dr. Martine Denicourt, DVM, is an invited professor at the Faculté de médecine

vétérinaire, where she teaches swine production management with great enthusiasm and is

also a member of CRIPA.

Congratulations to Dr. Denicourt for her promotion!

6 Info-CRIPA January 2014 I No 7

Marcelo Gottschalk, Université de Montréal

In collaboration with Drs. Mariela Segura, Serge Rivest (Université Laval) and Jiangun Xu (Stage Key Laborato-

ry, CDC Beijing), Dr. Marcelo Gottschalk was able to receive funding from the China-Canada Joint Health

Research Initiative program of the Canadian Institute of Health Research (CIHR). The project is called Canada-

China studies on the emerging zoonotic agent Streptococcus suis.

In collaboration with their Chinese colleagues, Drs. Mariela Se-

gura and Marcello Gottschalk were the primary organizers of

the first international symposium on S. suis in China. This sym-

posium was held in Beijing last August and brought together ap-

proximately 100 international experts on S. suis.

This symposium also included the participation of two researchers from

the Université Laval, Drs. Daniel Grenier and Laetitia Bonifait. For

more information : http://www.crip.umontreal.ca/documents/documents/

FACTUEL_International%20Symposium%20on%20S%20suis_2013(1).pdf

With the participation from one of their laboratory alumni, Nahuel Fittipaldi of the Methodist

Hospital Research Institute, Drs. Segura and Gottschalk wrote Chapter 13 about meningi-

tis caused by Streptococcus suis, in a new book entitled: Meningitis - Cellular and molecu-

lar basis.

Notably, the cover picture is from their research team.

Dr. Marcelo Gottschalk received the Pfizer Prize of excellence in research which is award to

professors to recognize their efforts and productivity in research and the quality of their research

projects.

He was also the chairman of the Streptococcus suis : Disease and future challenge Symposium

held from May 19 to 24 in Cuba.

Mariela Segura , Université de Montréal

As a winner of a Québec women of distinction award in 2012, Dr. Segura was

interviewed and the video is available on YouTube at http://www.youtube.com/watch

feature=player_embedded&v=xdhWUxJPFCg

J. Daniel Dubreuil, Université de Montréal

In June 2013, Dr. J. Daniel Dubreuil was named the president

of the Canadian Society of Microbiologists (CSM).

Dr Dubreuil published a review article in Toxins 2013 entitled:

Antibacterial and Antidiarrheal Activities of Plant Products

against Enterotoxinogenic Escherichia coli. that assembles the

sparse information available about the anti-diarrheal activity of

various plant products that also have an effect on diarrhea

caused by enterotoxigenic E. coli (ETEC). (5(11) 2009-2041;

doi:10.3390/toxins5112009.)

http://www.mdpi.com/2072-6651/5/11/2009

7 Info-CRIPA January 2014 I No 7

Christian Klopfenstein, Centre de développement du porc du Québec

On March 14 2013, Drs. Christian Klopfenstein from CDPQ and Dora Rodriguez from CQVB organized

an information session for veterinarians and swine producers about the disease surveillance systems and

tools developed at the regional, provincial, and federal levels that allow for real time disease surveillance.

Dr. Sylvie D’Allaire and Marie-Ève Lambert presented a seminar entitled “La FMV et la surveillance du

SRRP” demonstrating that molecular tools are not just important for rapid intervention, but also for the

long term fight against the PRRS virus.

An overview of this meeting was published in a “Bio Trends” bulletin by the CQVB and was written by Drs

Klopfenstein and Cécile Crost. The publication is available at no cost and the title is: Monitoring swine

diseases in Canada: Databases and information systems.

In French: http://cqvb.net/Documents_en_ligne/Publications_gratuites/BTD-13-07_SystemesInformation.pdf

In English : http://cqvb.net/Documents_en_ligne/Publications_gratuites/BTD-13-07A_SystemesInformation.pdf

Steve Charette, Université Laval

A study from the laboratory of Steve Charette focused on the role of

multilamellar bodies rejected by amoebas was featured on the cover of

the October 2013 issue of Eukaryotic cell.

http://ec.asm.org/content/12/10/1326.abstract?sid=3462dd6f-c4d4-4fe5-be0b-

dc3710f38e5f

Philippe Fravalo, Université de Montréal

Dr. Philippe Fravalo was named to the “Évaluation des risques biologiques dans

les aliments” committee of ANSES (France) for a three year mandate.

A most probable number (MPN) based method named “mini-MSRV” , for Salmonel-

la quantification in the agri-food context that was developed by Philippe Fravalo

as part of Dr. Salvat’s team at the AFSSA in France, is now included in the ISO-

6579 standard in Europe .

ht tp://onl ine l ibrary.wi ley.com/doi /10.1111/j .1745 -4581.2003.tb00031.x/abstract;jsessionid=34607C57C6FD6EE5EACE6B40F1CFB18F.f01t02

In partnership with Société Olymel and F. Ménard inc., Dr. Fravalo also received funding from

the Collaborative Research and Development program managed by the Natural Sciences and En-

gineering Research Council of Canada (NSERC).

The research project is entitled “Le procédé d’abattage/découpe des porcs sélectionne Listeria

monocytogenes” and will involve collaborations with Drs. Ann Letellier, Mario Jacques,

Sylvain Quessy and four Health Canada representatives.

Charles M. Dozois, INRS-IAF

Dr. Charles M. Dozois is a member of the National Organizing Committee for the International Union

of Microbiological Societies (IUMS). He acts as the Co-Chair of the scientific program and Canadian

Vice-Chair of the XIVth International Congress of Bacteriology and Applied Microbiology that will take

place in 2014.

8 Info-CRIPA January 2014 I No 7

Marie Archambault, Université de Montréal

Dr. Archambault co-authored the second edition of the Clinical Veterinary Microbiology

textbook. This book discusses the collection, isolation and culturing of clinical specimens

and provides detailed information about biochemical, serological and other tests used to

identify and differentiate pathogens of veterinary importance.

The bacteriology section discusses antibiotic resistance in pathogens of veterinary importance. This work is the result of close collaborations with the University College

Dublin and Irish Equine Center in Ireland.

She also gave an interview for the Veterinarius, the official journal of the “Ordre des médecins vétéri-

naires du Québec”.

During the fall of 2013, Dr. Archambault also took part in a mandatory training session focused on

the judicious use of antibiotics for veterinarians in the context of the provincial strategy for the health

and well being of animals.

Jean-Pierre Vaillancourt, Université de Montréal

From March 19 to 23 2013, Dr. Jean-Pierre Vaillancourt was in Mexico to lead a research team responsible

for the surveillance of an epidemic of highly pathogenic avian influenza (H7N3) in the state of Guanajuato.

Dr. Vaillancourt also participated in a discussion panel about recent initiatives and innovations in

re-emergent infectious diseases at the international France-Québec-Afrique conference. This event, organized

by the INSPQ, was held at the National Museum of Fine Arts of Québec in Québec from the 1st to 3rd of Octo-

ber 2013. In addition, he hosted a workshop about the creation of a zoonosis monitoring program.

Jérôme del Castillo, Université de Montréal

Dr. Jérôme del Castillo wrote Chapter 15 of the 5th edition of Antimicrobial Therapy in

Veterinary Medicine, a reference book for veterinary practitioners. This chapter was about

tetracyclines.

John M. Fairbrother, Université de Montréal

Dr. John M. Fairbrother developed an online database for animal pathogenic and zoonotic E. coli

(APZEC) that is a dynamic and user-friendly epidemiology tool for the surveillance of virulence and an-

timicrobial resistance of pathogenic E. coli.

This unique database is accessible for everyone and offers virulence gene and antimicrobial resistance

profiles of APZEC found in production ani-

mals, pets, wild life, environment and pro-

cessed food.

Since 2008, data associated with 2600

isolates have been collected from different

animal species in Québec, including

swine, cattle and poultry.

For more information : www.apzec.ca

Dr. Fairbrother also wrote a review article :

Fairbrother, J.M. 2013. Literature review on the control of Verotoxigenic E. coli (VTEC) in

food-producing animals for the OIE Animal Production Food Safety Working Group (APFSWG),

36pp.

9 Info-CRIPA January 2014 I No 7

Members of CRIPA cited in the Porc Québec magazine

The Canadian Swine Health Board (CSHB) financed several research projects on swine health which in-

cluded the improvement of diagnostic tools, whole genome sequencing of the PRSS virus and virus survi-

val in biofilms. The investigators for these projects were Drs. Josée Harel, Carl Gagnon and Mario

Jacques.

http://www.leseleveursdeporcsduquebec.com/upa_porcs_f i les/federat ions/pdf/centre_de_doc/

final_sans_pub_porc_quebec_avril_2013.pdf

Three artciles written by Dr. Ann Letellier, holder of the NSERC Industrial Research Chair in Meat Sa-

fety, and her technical assistant, Dr. Nadia Bergeron, have been published in a special report on the

judicious use of antibiotics in the December 2013 edition of the “Porc Québec” Magazine.

L’ABC des antibiotiques : http://www.crip.umontreal.ca/documents/documents/L'ABC%20des%20antibiotiques.pdf

Des solutions de rechange pour lutter contre la résistance des bactéries aux antibiotiques :http://

www.crip.umontreal.ca/documents/documents/Des%20solutions%20de%20rechange%20pour%20lutter%20contre%20la%20r%C3%A9sistance%20des%20bact%C3%A9ries%20aux%20antibiotiques.pdf

Une Chaire de recherche en salubrité des viandes au service des éleveurs : http://www.crip.umontreal.ca/documents/

documents/Une%20Chaire%20de%20recherche%20en%20salubrit%C3%A9%20des%20viandes%20au%20service%20des%20%C3%A9leveurs.pdf

The December 2013 edition also includes an article presenting the research done in the CRIPA as

well as an article that summarizes the research progress on the swine microbiome presented du-

ring the 2013 CRIPA symposium.

Le CRIPA : l’avant-garde québécoise d’un porc en santé ! : http://www.crip.umontreal.ca/documents/documents/Le%

20CRIPA%20-%20L'avant-garde%20qu%C3%A9b%C3%A9coise%20d'un%20porc%20en%20sant%C3%A9.pdf

Les microbes, pas que des ennemis pour le gain de poids des porcelets : http://www.crip.umontreal.ca/documents/

documents/Les%20microbes,%20pas%20que%20des%20ennemis%20pour%20le%20gain%20de%20poids%20des%20porcelets.pdf

A University Laval research team lead by Dr. Frédéric Guay investigated the use of two strate-

gies to reduce the feed cost associated with fattening pigs.

Le gain compensatoire : gagnant sur tous les plans ! : http://www.crip.umontreal.ca/documents/documents/Le%

20gain%20compensatoire%20-%20gagnant%20sur%20tous%20les%20plans.pdf

Finally, Dr. Christian Klopfenstein and his colleague, Élise Gauthier, developed an informa-

tic tool for the management of sanitary conditions in the swine industry.

Un « portail santé » pour optimiser le travail sanitaire sur le terrain. : http://www.crip.umontreal.ca/

documents/documents/Un%20portail%20sant%C3%A9%20pour%20optimiser%20le%20travail%20sanitaire%20sur%20le%20terrain.pdf

Martine Boulianne, Université de Montréal

Dr. Martine Boulianne was the editor in chief of the 7th edition of the Avian Disease

Manual, a publication by the American Association of Avian Pathologists (AAAP).

Under the direction of Dr. Boulianne, this book of over 300 pages was completely updated.

The superb cover photo is the work of Marco Langlois.

http://www.aaap.info/avian-disease-manual

10 Info-CRIPA January 2014 I No 7

Sébastien Sabbagh, Université de Montréal

Sébastien, a Ph.D. student and CRIPA member in the laboratory of Dr. France

Daigle, was interviewed by “Les années lumière” a radio segment dedicated

to science at Radio-Canada.

http://www.radio-canada.ca/emissions/les_annees_lumiere/2013-2014/chronique.asp?idChronique=320109

Yannick Tremblay, Université de Montréal

Yannick, a post-doctoral trainee in the laboratories of Drs. Mario Jacques and

Josée Harel, presented their most recent findings on November 26 2013 in a

webinar entitled : “L'impact des biofilms dans la salubrité alimentaire et la santé

animale”.

The webinar was organized by the “Centre québécois de valorisarion des biotech-

nologies”, the United States-Mexico Foundation for Science (FUMEC), and TECH-

PYME alimentos, in collaboration with CRIPA and the “Cité de la biotechnologie de

St-Hyacinthe”. Yannick was one of the featured speakers, he spoke alongside

Philip S. Stuart from Montana State University and Alejandro Castillo from Texas

A&M University. Their presentations allowed for a better understanding of the

impact of biofilm-formation on the efficacy of disinfection (from the farm to the

table) and on the treatment of infections.

Marie-Lou Gaucher, Université de Montréal

Dr. Gaucher presented the results of her Ph.D. project entitled: “Tests de pro-

duction à grande échelle de poulets sans antibiotiques” during an AQINAC

meeting, a poultry industry event that brings producers and stakeholders to-

gether.

Marie-Lou performed her doctoral research in the laboratory of the research

chair in meat safety in collaboration with Dr. Martine Boulianne from the

“Chaire en recherche avicole”. The results of her project were of great inte-

rest to members of the poultry industry.

Our Trainees Shine

CRIPA Activities in 2013

A training project run with the collaboration of CRIPA members quali-

fied for Science on Stage Canada

Over 350 science teachers from 25 countries presented their teaching philosophies at the

Science on Stage festival held from April 25 to 28 in Stubice (Poland) and Frankfurt

(Germany). Among the 6 competitors from Canada, the project “Design pédagogique d’un mi-

crostage en recherche au pré-universitaire en Science de la nature: de l’intention à l’action”

distinguishes itself by the opportunity offered to students spend 2 days observing the daily

life of researchers in universities, the government or industries. This project was achieved

through the collaboration of CRIPA members and was presented by Huguette Thibeault, a

biology teacher at the Cégep de Saint-Hyacinthe.

11 Info-CRIPA January 2014 I No 7

81st ACFAS conference

The CRIPA organized two activities associated with the ACFAS confe-rence on May 8th and 9th 2013 at the Universite Laval in Québec City.

On May 8, the activity focused on the symposium “Colloque 227-Le

microbiote animal : une question d'équilibre!”

The presentation was given by Richard Isaacson (University of Minnesota), Janet Hill

(University of Saskatchewan), Évelyne Forano (INRA de Theix), Janet MacInnes

(University of Guelph) and Guylaine Talbot (CRDBLP, AAC).

On May 9, the CRIPA held its 6th Annual Symposium which detailed the research ac-

tivity of the CRIPA. Dr. Marie-Rose Van Calsteren and our students presented

their results orally and a poster session was also held.

Fernando Alvarez from the FMV (supervised by Dr. Carl A. Gagnon) received the

1st prize for the best oral presentation followed by Pascale Blais-Lecours from

Université Laval (cosupervised by Dr. Caroline Duchaine). The 1st prize for best pos-

ter presentation was awarded to Nissanka Nilmini Mendis of McGill University

(supervised by Dr. Sébastien Faucher) followed by Kathleen Charles of the INRS-

IAF (supervised by Dr. Charles M. Dozois).

2nd symposium of the BiSP/Integrative Bacteriology

Over 100 individuals participated in the symposium with an excellent program entitled, Integrated

Bacteriology: Symbiosis and pathogenesis (2nd edition). This symposium, which was started by Dr.

Steve Charette, a professor at the Universté Laval, was orga-

nized in collaboration with the CRIPA. The event was held at

the Université Laval on November 28 and 29 2013. In addi-

tion to presentations by two guest speakers (Didier Bouchon

and Martin Filion), the program included 19 oral presenta-

tions and 26 poster presentations.

Several students from CRIPA shined in their presentations,

notably Nissanka Mendis of McGill University (Laboratory of

Sébastien Faucher) who obtained the prize for the best pre-

sentation by a master’s student. From the FMV, Philippe

Vogeleer, a PhD student (supervised by Josée Harel) won

the same award in the PhD student category. Laetitia Boni-

fait (Laboratory of Caroline Duchaine) and Julie Jeukens (Laboratory of Roger Levesque), both from

the Université Laval, shared ex aequo the prize in the Postdoc category. Prizes for the best oral

presentations were offered by CRIPA. Other students from our center also shined with their pos-

ter presentations: Bruno Haas and Marie-Pierre Morin, who both won top prizes, are members

of Dr. Daniel Grenier’s (U. Laval) laboratory.

The CAFÉ-CRIPA: Mai 29, 2013

On May 29 2013, we held our 3rd Café-CRIPA at the Faculté de médecine vétérinaire of

the Université de Montréal. This event was organized in collaboration with the FMV

diagnostic service under the direction of Dr. Estella Cornaglia and within the context

of the annual conference of the Canadian Animal Health Laboratorians Network

(CAHLN), which was presided this year by Dr. Cornaglia.

The themes of the meeting included the porcine respiratory and reproductive syndrome

virus (PRRSV), Mycoplasma hyonoris, Brachyspira and S. suis. These subjects were pre-

sented by Drs. Carl A. Gagnon (UdeM), Jane C. Hennings (University of Minnesota), John

Harding (University of Saskatchewan), Marcelo Gottschalk (UdeM) and Maria Calvijo (University of Minne-

sota).

12 Info-CRIPA January 2014 I No 7

Lunch conferences at the CRIPA/GREMIP

Eleven conferences were given to our students, members and guests of the CRIPA and the GREMIP.

These presentations, given by high-level researchers, are always an opportunity to acquire new

knowledge in a friendly atmosphere.

The presentations that were held during the last year are as follow:

Speakers Dates Titles

2012

Sébastien P. Faucher McGill

November 22 Legionella pneumophila : une histoire d'eau

Nahuel Fittipaldi University of Toronto

December 7 Whole-Genome Sequencing Analysis of a Continuing Group A Streptococcus Epidemic

Isabelle Oswald INRA, Toulouse, France

December 12 Le porc : animal cible et modèle pour l'étude des my-cotoxines

2013

Caroline Duchaine U Laval

February 14 Bioaérosols dans les porcheries : un modèle de choix!

Eric Déziel INRS-IAF

April 10 The various lifestyles of the Burkholderia cepacia com-plex species: a tribute to adaptation

Catherine Paradis-Bleau U Montréal

April 25 Découverte de nouveaux facteurs importants pour la biosynthèse de l'enveloppe bactérienne

Melha Mellata Arizona State University

September 4 Understanding the virulence and zoonotic risk of Ex-traintestinal Pathogenic E. coli and vaccine design strategies their infections in both humans and poultry

Ismail Fliss U Laval

September 26 Les bactéries lactiques bactériocinogènes : une nou-velle génération de probiotiques à fort potentiel pour les secteurs médical et vétérinaire

Dao Nguyen U McGill

October 3 Antibiotic tolerance in Pseudomonas aeruginosa : to die or not to die

Sarah Chuzeville ANSES, INRA, France

November 12 Caractérisation des fonctions codées par les éléments intégratifs et conjugatifs (ICE) intégrés dans un gène codant un ARNt lysine chez Streptococcus agalactiae

Fadia Naim Cintech

December 4 CINTECH, illustration d’un réseau CTT sur la valorisa-tion et la sécurité alimentaire

13 Info-CRIPA January 2014 I No 7

Molecular Epidemiology Workshop - Multidisciplinary Workshop

Under the initiative of Dr. Simon Dufour, director at the Canadian Bovine Mastitis and Milk Quality

Research Network (CBMQRN), and in collaboration of CRIPA and GREZOSP, Drs. Julio Álvarez Sánchez

of VISAVET Health Surveillance Centre of the Complutense, Univer-

sity of Madrid and Andres Perez of the Center for Animal Disease

Modeling and Surveillance, UC Davis, US, gave a workshop en-

titled: "Understanding molecular data – from nucleotides to mole-

cular-based surveillance and source attribution". This workshop

was held at the Faculté de médecine vétérinaire on November 18

to 20 2013.

The training taught the basics of molecular epidemiology and ex-

plored validated biofinformatics tools and approaches commonly

used for innovative research. The success of this workshop was

due to the combined efforts of all the collaborators.

OPIC Workshop - Multidisciplinary Workshop

A workshop entitled “Les rudiments de la propriété intellectuelle: quels avantages peut-elle vous offrir?”

was presented by Serge Lapointe (Fasken Martineau) and Lynda Adam (UMontréal) on April 18 2013.

Doctoral Theses

CRÉPIN, Sébastien. 2012. Rôle du système de transport du phosphate Pst et du régulon Pho

dans la virulence de la souche E. coli uropathogène CFT073. Centre INRS-Institut

Armand-Frappier. Supervisor: Charles M. Dozois, Cosupervisor: Josée Harel

FOREST, Chantal. 2012. Caractérisation des fimbriae de Salmonella enterica serovar Typhi.

Université de Montréal. Supervisor: France Daigle

SABBAGH, Sébastien. 2013. Identification de mutants essentiels à la survie de Salmonella

enterica serovar Typhi dans les macrophages. Université de Montréal. Supervisor: France

Daigle

THIBODEAU, Alexandre. 2013. Caractérisation phénotypique et génotypique de Campylobac-

ter jejuni et évaluation d’une stratégie de contrôle de la colonisation du poulet de chair par

ce pathogène alimentaire. Université de Montréal. Supervisor: Ann Letellier, Cosupervi-

sors: Sylvain Quessy, Évelyne Guévremont

In 2013, these workshops specifically targeted the CRIPA students

Best in Genomics - Technological Workshop

This workshop aimed at developing entrepreneurial skills in genomics. The objective was to promote and

maximize the translation of innovative research work and results into profitable commercial applications.

This workshop was presented by Carole Pumas and Marie-Eve Dextraze on October 24 2013 at the FMV.

Technological and multidisciplinary workshop

Graduate of the CRIPA

14 Info-CRIPA January 2014 I No 7

Doctoral Theses (continued)

TREMBLAY, Cindy-Love. 2013. Étude de la résistance aux antibiotiques des entérocoques d'origine

animale du Québec. Université de Montréal. Supervisor: Marie Archambault, Cosupervisors:

Ann Letellier, Jérôme del Castillo

Masters Theses

ALVAREZ, Fernando. 2013. Création d’un modèle cellulaire des voies respiratoires du porc pour étudier

les effets d’une co-infection virale au virus du syndrome reproducteur et respiratoire porcin et au

circovirus porcin. Université de Montréal. Supervisor: Carl A. Gagnon

BOUCHARD, Marilyne. 2013. Caractérisation de la salmochéline chez Salmonella enterica serovar Typhi.

Université de Montréal. Supervisor: France Daigle

CLARKE, Damian. 2012. Role of CD4+ T cells in the regulation of the immune response against encapsu-

lated Group B Streptococcus. Université de Montréal. Supervisor: Mariela Segura

DANG, Khanh B. 2012. Detection and quantification of Staphylococcus aureus enterotoxin B in food

product using isotopic dilution techniques and mass spectrometry. Université de Montréal.

Supervisor: Ann Letellier

DAVID, Élise. 2012. Caractérisation et délétion de tous les systèmes d'adhésion connus de Salmonel-

la enterica sérovar Typhi. Université de Montréal. Supervisor: France Daigle

FERCHICHI, Salma. 2013. Pharmacocinétique de population du propofol chez le chien. Université de

Montréal. Supervisor: Jérôme del Castillo

LARIVIÈRE-GAUTHIER, Guillaume. 2013. Détection et caractérisation génétique de Listeria

monocytogenes dans une usine d’abattage/découpe de porcs au Québec. Université de Montréal.

Supervisor: Ann Letellier. Cosupervisors: Philippe Fravalo, Sylvain Quessy

LAVOIE, Rémi. 2013. Étude de la variation de phase des fimbriae F1651, Pap et CS31A et de l’im-

pact des régulateurs homologues de PapI. Université de Montréal. Supervisor: Josée Harel,

Cosupervisor: Christine Martin

LÉTOURNEAU, Jason. 2013. Les bactéries exprimant AIDA-I interagissent avec l'apolipoprotéine

A-I cellulaire. Université de Montréal. Supervisor: Michaël Mourez

NGENDAHAYO MUKIZA, Clément. 2012. L’entérotoxine STb d’Escherichia coli affecte les

jonctions serrées des cellules intestinales épithéliales. Université de Montréal. Supervisor:

J. Daniel Dubreuil

PELLETIER-JACQUES, Geneviève. 2012. Étude de la virulence et de la résistance aux

antibiotiques des Staphylococcus aureus résistants à la méthicilline chez le porc à l'abattoir au

Québec. Université de Montréal. Supervisor: Marie Archambault

PHAM, Thu Minh. 2013. Potential pathogenicity and antimicrobial resistance of Escherichia coli

from pig and poultry feces on-farm and carcasses at the abattoir in Vietnam. Université de

Montréal. Supervisor: John Morris Fairbrother, Cosupervisor: Ann Letellier

TRAN, Thi Phuong Hoa. 2013. Impact of pesticides on indicator and pathogenic microorgan-

ism persistence under laboratory and field conditions. Université de Montréal. Supervisor:

Ann Letellier

15 Info-CRIPA January 2014 I No 7

FRQNT International Internship Bursary

Jean-Philippe Auger : En route to the United Kingdom!

This four and a half month internship was hosted by the laboratory of Dr. Myron Christodoulides at the

Faculty of Medicine at the University of Southampton, Southampton

General Hospital, in Southampton (United Kingdom). Dr. Christodou-

lides is recognized as an expert in the pathogenesis of bacterial

meningitis, more specifically, he studies the interaction between

human meninges cells and bacteria. His laboratory focuses their

research on Neisseria meningitidis, an important bacterial pathogens

responsible for meningitis in humans.

The aim of this internship was to study the interaction between

different Streptococcus suis serotype 2 strains and mutants and

human meningeal cells. This bacterium is an important swine pathogen and an emerging zoonotic

agent, and is implicated in hundreds of meningitis cases every

year worldwide, including two epidemics in China. Despite the

importance of meninges cells during a bacterial infection of the

nervous system, the interaction between these cells and S. suis,

which can cause meningitis, has never been investigated.

This study used meninges cells isolated from human meningio-

mas and these cells have been used for several years by Dr.

Christodoulides to study the human pathogens responsible for

meningitis including N. meningitidis, E. coli K1 and group B

streptococcus (GBS). Different S. suis isogenic mutants for well-

characterized virulence factors, and human and swine isolates

representing different allelic types (ST) that are important

worldwide were used for our study and we also included the

strain responsible the 2005 human outbreak in China. Adhesion, invasion and cytotoxic effects

were measured for the different strains used, as well as the inflammatory response of the cells

when infected with the different strains.

The results revealed that different STs, as wells as certain mutants and their respective parental

strain adhered differently to meninges cells. Unlike several other bacteria studied before, none of

the S. suis strains, including the strain responsible for the Chinese outbreak, had cytotoxic ef-

fects on the meninges cells and the ability of S. suis to invade these cells was intermediate. Fur-

thermore, S. suis induced low levels of pro-inflammatory cytokines and these levels were lower

than those induced by N. meningitidis. Despite their importance during a bacterial infection,

meninges cells, which are more resistant to S. suis, are probably not the main source of inflam-

mation observed during meningitis. However, meninges cells can interact with S. suis. The re-

sults obtained during my internship will need to be validated in an in vivo model and compared

to those obtained with pork, the natural host of S. suis.

The experiments performed during my internship allowed me to study the interaction of

S. suis with a new cell type which was not previously used and would be hard to access under

normal circumstances. The results will be published in a scientific article that is currently in

preparation.

These newly acquired cell culture skills will be advantageous to try to develop a cell model

for porcine meninges. Furthermore, these techniques will allow me to broaden the scope of

my past and future results, which will allow for the advancement of knowledge required to

develop preventative and therapeutic treatments and for diagnostic purposes.

Bursaries

16 Info-CRIPA January 2014 I No 7

Finally, this internship allowed me to cement a collaboration that will improve the international reputation

of Dr. Gottschalk’s laboratory, the CRIPA, and the Université de Montréal while increasing our under-

standing of S. suis pathogenesis.

This collaboration between the laboratories of Drs. Gottschalk and Christodoulides could provide new

avenues for partnership for the laboratory of Dr. Gottschalk, potentially including the CRIPA, with the

University of Southampton. The future development of a porcine me-

ninges model will be influenced by the cell culture techniques acquired

during my internship and the techniques themselves are priceless as-

sets for our laboratory and will be available to members of CRIPA.

Jean-Philippe Auger

I thank the Fonds de recherche du Québec—Nature et technologies for the

financial support for this internship.

FRQNT International Internship Bursary

Guillaume Le Bihan : En route to France!

My two month long internship was held in the Unité Mixte de recherche

UR454 of INRA (Institut National de Recherche en Agronomie) in Cler-

mont-Ferrand-Theix, France under the supervision of Drs. Christine

Martin and Grégory Jubelin, in collaboration with Dr. Annick Bernalier-

Donadille. The UR454 research team studies the adaptation of

enterohemorrhagic Escherichia coli (EHEC) to the digestive environ-

ment and the impact of the intestinal microbiome on the nutrition and health of the host.

The objective of my internship was to study the adaptation of EHEC O157:H7 strain EDL933 to the

intestinal environment conditioned by the human intestinal microbiome. EHEC strains are food-

borne pathogens that infect the human intestine resulting in important morbidity and, in some

cases, mortality. EHEC are Shiga toxin producing E. coli (STEC) and some STEC are swine patho-

gens that are responsible for edema disease (ED) in recently weaned piglets. EHEC strains are

normally commensal bacteria in cows but can cause bloody diarrhea and hemolytic-uremic syn-

drome in humans. Strains from the O157:H7 serotype are those most frequently isolated from

clinical cases. To colonize the intestinal tract, EHEC must adapt to the presence of the intestinal

microbiome. My internship allowed me to study the transcriptional response of EDL933 cultured

in intestinal medium conditioned or not with the intestinal microbiome with the aim of discover-

ing the adaptation strategies used by this pathogen.

I was able to take full advantage of the expertise of the INRA research team UR454

who developed a model to study the human intestinal microbiome in germ-free rats.

These rats are carefully controlled by the laboratory of Dr. Annick Bernalier-Donadille to

ensure the colonization and persistence of the human intestinal microbiome.

EHEC strain EDL933 was cultured in vitro in the caecal content of the germ-free rats

and rats colonized with the human fecal microbiome. Gene expression was measured

for EDL933 cultured under both intestinal-content conditions. A focus was put on the

genes involved in EHEC virulence and metabolism. Furthermore, an analysis of the fecal

content helped measure the concentration of several compounds important for EHEC.

17 Info-CRIPA January 2014 I No 7

Recipient of CRIPA bursary in 2013

Need-based Bursary

Master student recipient: Doctoral student recipient:

Vicente Andres Pinilla Ramirez (UdeM) Guillaume Goyette-Desjardins (UdeM)

Joseph Saoud (INRA-IAF) Devin Holman (UMcGill)

Kathleen Sary (UdeM) Philippe Vogeleer (UdeM)

Post-doctoral recipient:

Laetitia Bonifait (ULaval)

Travel Bursary – Again this year, eleven bursaries were awarded to our students:

Vicente Andres Pinilla Ramirez (UdeM) Gabriel Desmarais (UdeM)

Myriam Ouellette (ULaval) Flavien Ndongo UdeM)

Paul Lemire (UdeM) Philippe Lebel (UdeM)

Audrey Charlebois (UdeM) Laam Li(UMcGill)

Philippa Perrot (ULaval) Gaëlle Porcheron (INRS-IAF)

Mylène Blais (AAC-Lennoxville)

Congratulation to all trainees !

The transcrptomic data demonstrated that the metabolic state of EDL933 is very

different when exposed to the fecal content of rats conditioned with the human

intestinal microbiome in comparison to the fecal content of germ free rats. In the

caecal content of human-conditioned rats, EDL933 down-regulates its sugar ca-

tabolism genes but up-regulates its genes associated with the use of secondary

energy sources that are produced by the metabolic activity of the microbiome.

Metabolic analysis revealed that the intestinal microbiome modifies the intestinal

content by significantly limiting the concentration of several nutrients. Further-

more, our results demonstrate a down-regulation of virulence genes in the caecal

content from human microbiome-conditioned rats suggesting that the microbiome

may offer a protection against EHEC infections.

The findings that arose from my internship have been presented at several conferences and a manuscript

has recently been submitted. Furthermore, a follow up study is under way that is looking into the mecha-

nisms used by the intestinal microbiome to inhibit EHEC virulence. Ultimately, the study of adaptation of

EHEC to the intestinal environment and the mechanisms used by the intestinal microbiome to inhibit

EHEC virulence can result in new strategies to prevent and/or treat EHEC infections.

In addition to working with high quality and rigorous team of scientific experts, this internship has al-

lowed me to meet researchers with different specialties in an open and friendly atmosphere. Further-

more, the INRA center in Clermont-Ferrand-Theix is located in the heart of an exceptional region, Au-

vergne, a place to discover the welcoming atmosphere, good food and exceptional landscapes with

the occasional volcano.

Finally, this internship has allowed me to acquire new knowledge and expertise, and reinforce our

relationship with our collaborators.

Guillaume Le Bihan

I thank the Fonds de recherche du Québec—Nature et technologies for the financial support for this

internship.

18 Info-CRIPA January 2014 I No 7

New initiatives

2011 Competition

« Relation entre protozoaires aquatiques et survie d’Escherichia coli entérohé-morragique dans l’environnement »

France Daigle (FMV-UMontréal), Steve Charette (ULaval), Charles Dozois (INRS-IAF), Josée Harel (FMV-UMontréal)

E. coli is a bacterium that naturally colonizes the intestinal epithelium of humans and other animals. This

bacterium is considered a commensal but can also be associated with infections. A group of bacterial

pathogens, the attaching and effacing E. coli (AEEC) cause intestinal infections that are associated with

effacement of intestinal microvilli and diarrhea. Among AEEC, we find strains that infect swine and strains

that produce Shiga-toxin, which include E. coli O157:H7 (enterohemorrhagic E. coli or EHEC). In the last

few years, this last group has been associated with serious food-borne infections that may result in he-

molytic-uremic syndrome.

Domesticated ruminants are the main reservoir of E. coli O157:H7 and pigs are infrequent carriers. The

transmission of E. coli O157:H7 to humans is through food or water that has been contaminated with

feces from animals carrying this pathogen. EHEC can survive in surface water by expressing specific

genes that allow them to adapt to this environmental condition, therefore, drinking water is vulnerable

to EHEC contamination and represents a constant risk of outbreak. In the aquatic environment, proto-

zoans, including free amoebas, are known to host and protect several pathogens from the water.

The research hypothesis was that amoebas found in water could be a reservoir of E. coli O157:H7.

Furthermore, water, which is a phosphate poor environment, could induce the expression of the Pho

regulon and other genes required for the survival of EHEC in amoebas.

The study demonstrated that the amount of E. coli O157:H7 was significantly larger when cultured

in the presence of amoebas than in their absence. Using E. coli O157:H7 derived mutants, the team

demonstrated that the Pho regulon was essential for the growth of EHEC in co-culture with amoe-

bas. However, Shiga toxins (Stx) prevented the internalization and survival of E. coli O157:H7 in

amoebas. This study suggests that protozoans play a role in the persistence of pathogenic E. coli in

the environment and this study could help prevent and protect against contamination by STEC.

2012 Competition

« Rôle des biofilms bactériens dans la persistance de virus pathogènes du porc dans l’environnement de la ferme »

Mario Jacques (FMV-UMontréal), Carl A. Gagnon (FMV-UMontréal), Daniel Grenier (ULaval)

A biofilm represents a mode of growth frequently used by bacterial pathogens because it pro-

tects bacteria during an infection. Furthermore, bacterial biofilms play a role in the transmission

of infections and the persistence of bacteria in the environment because biofilm formation is al-

so associated with an increased resistance to antibiotics and disinfectants.

This proposed project aimed at evaluating if bacterial biofilms can protect viruses and thus in-

crease their environmental persistence. The specific objectives were to: (1) determine if the

PRRS and PCV2 virus could persist within a bacterial biofilm; and (2) determine if bacterial

biofilms could offer a protection for the virus against commonly used disinfectants. The results

demonstrated that the virus could persist within a biofilm of respiratory bacteria

(Actinobacillus pleuropneumoniae and Streptococcus suis) and digestive bacteria (Escherichia

coli and Salmonella).

Furthermore, biofilms could reduce, to a lesser extent, the efficacy of disinfectants used in

this study.

19 Info-CRIPA January 2014 I No 7

Research Progress

Raising Antibiotic-free chicken

The researchers at the Chaire en recherche avicole of the Faculté de médecine vétérinaire of the Univer-

sité de Montréal, Drs. Martine Boulianne and Marie-Lou Gaucher, that were responsible for this project

presented their results before the summer of 2013 to the farmers that participated in the “Tests de pro-

duction à grande échelle de poulets sans antibiotiques”. Then the members of the EVQ and stakeholders

in the poultry industry learned about the results during the fall of 2013.

The researchers indicated that it is possible, under certain conditions, to raise chickens without antibiot-

ics with a higher but reasonable cost than conventional farming. However, it was not demonstrated that

this was possible at on a larger scale.

In fact, some farms had their birds raised without antibiotics performed as well as those raised in a con-

ventional fashion but other farms had recurring problems with enteritis despites using the same strate-

gies as successful farms. The researchers think that a harmful bacterial flora is present on farms with

problems and further analyses will be required to understand the phenomenon and determine the key

factors for success before starting large scale farming of chicken without antibiotics.

Furthermore, new data helped develop a better understanding of the needs of young chicks and high-

light the importance of a multifaceted approach to maintain the digestive health of the birds. These

data will be very useful to the improve farming methods in the poultry industry. A big thank you to

the participating farmers.

During a meeting, Drs. Boulianne and Gaucher thanked on behalf of themselves and the research

committee the farmers that participated in the study. The collaboration and availability of the farm-

ers were key assets for the success of this project. In fact, eight farmers precisely followed a re-

search protocol for over a year and gave access to two rearing houses each, one used for conven-

tional farming and the other for farming without antibiotics, to the researchers for visiting the farms

regularly, for collecting samples and for measuring the health and growth performance of the birds.

The committee will determine if there will be a follow up to the project. The project “Tests de pro-

duction à grande échelle de poulets sans antibiotiques” was financed by Agriculture and Agrifood

Canada through Canadian Agricultural Adaptation Program (CAAP). In Quebec, the part of this pro-

gram aimed at the agricultural production sector was managed by the Conseil pour le développe-

ment de l’agriculture du Québec (CDAQ).

Source: Le Provoqué Bulletin mensuel des Éleveurs de volailles du Québec

June 2013

20 Info-CRIPA January 2014 I No 7

Swine Cluster Project involving CRIPA members (2010-2013) “Efficacy of feed additives to mitigate the negative im-

pacts of mycotoxin contaminated feed on the perfor-mance and health of piglets”

This project is supervised by the following CRIPA members:

Frédéric Guay, Université Laval, Québec

Martin Lessard, Centre de recherche et

de développement sur le bovin laitier et le

porc, Agriculture et Agroalimentaire Cana-

da (AAC), Sherbrooke

Younes Chorfi, Faculté de médecine

vétérinaire, Université de Montréal, Saint-

Hyacinthe

http://www.innovationporc.ca/a-3.php

“The use of tools related to molecular characterization, systemic analysis

of stakeholders and geomatics for identification of the principal vectors and contamination sources by bacteria and viral indicators at farm and

slaughterhouse level”

This project is under the leadership of

Ann Letellier, holder of the Research

Chair in Meat Safety, Université de Mon-

tréal, Faculté de médecine vétérinaire,

Saint Hyacinthe.

http://www.innovationporc.ca/b-14.php

Research Progress

21 Info-CRIPA January 2014 I No 7

Scientific communication by the CRIPA in 2014 We invite you to participate in our scientific communication activities in 2014! Please mark your calendar and read the CRIPA Bulletin for more information

and to register.

February 25, 2014 in Longueuil Café-CRIPA on VACCINES - Improving vaccine development: from research to international

markets

June 3-4, 2014 at the FMV Saint-Hyacinthe 7th CRIPA Annual Symposium