inger martinussen, vladimir volodin, gunnlaug røthe, kirsten jakobsen and hermod nilsen
DESCRIPTION
Effect of climate on plant growth and level of adaptogenic compounds in Rhodiola rosea , Leuzea chartamoides and Serratula coronata. Inger Martinussen, Vladimir Volodin, Gunnlaug Røthe, Kirsten Jakobsen and Hermod Nilsen. BARENTSHERBS. Controlled experiment in a phytotrone. - PowerPoint PPT PresentationTRANSCRIPT
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Effect of climate on plant growth and level of adaptogenic compounds in Rhodiola rosea, Leuzea chartamoides and Serratula coronata Inger Martinussen, Vladimir Volodin, Gunnlaug Røthe, Kirsten Jakobsen and Hermod Nilsen
BARENTSHERBS
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Controlled experiment in a phytotrone
24 h 12 h
9°C
15°C
21°C 21°C
21°C day9°C
night
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Temperature effects on growth of Rhodiola
TEMP CSHOOTS
P<0.0001D.W. (g) ROOT
P<0.007**
9 28.3 B 17.6 BA
15 30.3 B 21.9 BA
21/9 40.3 A 24.1 A
21 12 h 12.3 C 13.2 B
21 24 h 22.5 B 15.4 BA
N = 30 pr treatment1 clone Rovaniemi1 clone Akershus1 clone Tromsø
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Temperature effects on adaptogens in Rhodiola
TEMP C
Cinnamic alcohol
P<0.796
RosavinP<0.873
TyrosolP<0.001**
Salidroside
P<0.830
Total rosavinesP<0.833
9 0.75
A 3.02 A 0.15 A 2.42 A 5.59
A
15 0.67
A 2.98 A 0.11 AB 3.34 A 4.96
A
21/9 0.72
A 2.66 A 0.09 B 2.85 A 4.66
A
21 12 h
0.61
A 3.29 A 0.06 B 3.27 A 4.97
A
21 24 h
0.66
A 2.94 A 0.06 B 2.98 A 4.75
A
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Clonal differences!
•The northernmost clone best•The trends are the same – the levels different
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Temperature effects on Leuzea
chartamoides
TEMP C HIGHT (cm) P<0.0001***
FLOWERING (NUMBER)
P<0.0001***
D.W. (g) ROOT P<0.0001***
9 53.0 C 0 C 1.7 C
15 68.5 A 0.4 B 5.5 A
21/9 69.8 A 0.5 B 4.2 B
21 12 h 30.2 D 0 C 1.4 C
21 24 h 59.7 B 1.3 A 2.4 C
N = ca. 40 pr treatment1 clone from Mikkili1 seed population from Mikkili
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Ecdysteroids in Leuzea chartamoides
•N = 8 pr treatment – combined from 5 samples
•Low at 9 ◦C•Highest at 15 ◦C and decreases at 21 ◦C
•Negative effect of long days at higher temperatures?
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Temperature effects on growth of Serratula coronata
TEMP C HIGHT (cm) P<0.0001***
FLOWERING P<0.0001***
D.W. (g) LEAF P<0.0001***
9 20.3 C 0 C 0.8 D
15 93.1 A 1.4 BC 11.4 B
21/9 80.0 B 2.9 A 8.2 C
21 12 h 17.2 C 0 C 0.9 D
21 24 h 81.8 B 2.0 BA 16.0 A
N = 40 pr. treatment2 seed populations
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Ecdysteroids in Serratula coronata
•N = 8 pr treatment – combined from 5 samples
• Short days as negative
•Increase in concentration from 9◦C to 15 ◦C
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Summary & conclusions
• Long days necessary for growth and flowering• Short days reduced the concentration of
ecdysteroids in Serratula (NB – tested only at high temperature)
• Long days reduced the concentration of ecdysteroids in Leuzea (NB – tested only at high temperature)
• Low temperature significantly increased the amount of tyrosol in Rhodiola and results shows a higher concentration of the other adaptogens also at low temperature (9◦C & 15◦C), however, not significant
• The northernmost clone had the highest content of adaptogens at all treatments
• The intermediate temperature of 15◦C gave the highest amount of ecdysteroids in both Leuzea and Serratula
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THANKS!• Institute of Biology, Komi Science Centre, Russia• MTT Rovaniemi, Finland• MTT Mikkili, Finland• Bioforsk – Svanhovd, Norway• Bioforsk – Kise• Bioforsk – Holt• UiTø
The Norwegian Barents SecretaryInterreg IIIAAgronomy County offices in Troms and Finnmark