insights from a formidable companion, the mudcrab · martín berón de astrada alejandro delorenzi...
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Insights from a formidable companion, the mudcrab Neohelice granulata
Julieta Sztarker
Universidad de Buenos Aires
IFIBYNE-CONICET
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Understanding from the entire animal to its minimal components
• Behavior (as pray, predator, optomotor reflexes)
• Organization of the brain centers (neuroanatomy: Bodian, Golgi, Dextran injections, intracellular)
• Identification of areas (lesion) / neurons (electrophysiology) in the brain responsible for integrating visual stimuli and commanding specific behaviors (escape, optomotor, tracking)
• Neuronal ultrastructure (synaptic organization)
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Escape responses Attack responses
Optomotor responses
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Understanding from the entire animal to its minimal components
• Behavior (as pray, predator, optomotor reflexes)
• Organization of the brain centers (neuroanatomy: Bodian, Golgi, Dextran injections, intracellular)
• Identification of areas (lesion) / neurons (electrophysiology) in the brain responsible for integrating visual stimuli and commanding specific behaviors (escape, optomotor, tracking)
• Neuronal ultrastructure (synaptic organization)
![Page 6: Insights from a formidable companion, the mudcrab · Martín Berón de Astrada Alejandro Delorenzi Daniel Tomsic Claire Rind Grants: Universidad de Bs As FONCyT CONICET EU RISE- STEPDYNA](https://reader033.vdocument.in/reader033/viewer/2022050411/5f87b88123299462773b3205/html5/thumbnails/6.jpg)
D
V
L M
recordingelectrode
In vivo intracellular recordings
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Movement detector neurons (LG), intracellular recording and staining
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EM analysis of connectivity of collision detector neurons:
MLG1
MLG1
MLG1Collaboration with Dr Rind
Newcastle University. EU RISE
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We have recently described a candidate region for optic flow analysis (lobula plate):
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Retinotopic connections
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Electrolytic lesions:
Lesion Behavior Confirmation
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Techniques:
• Behavior analysis• Intracellular recordings in vivo• Electrolytic lesions• Histology (Bodian, Golgi, Dextran impregnations,
3D reconstructions Neurolucida)• Electron microscopy
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Yair BarnatanFlorencia ScaranoMercedes BengocheaMaría Grazia LeporeFrancisco Maza
Martín Berón de AstradaAlejandro DelorenziDaniel TomsicClaire Rind
Grants:Universidad de Bs As
FONCyTCONICET
EU RISE- STEPDYNA