institute of biochemistry european monitoring center for ... · pdf fileeuropean monitoring...
TRANSCRIPT
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Activities of the European Monitoring Center for Emerging
Doping Agents
Mario Thevis
Institute of Biochemistry / Center for Preventive Doping Research , German Sport University Cologne, Germany
& European Monitoring Center for Emerging Doping Agents (EuMoCEDA), Cologne/Bonn, Germany
WADA / Science and Investigations Symposium, October 28th 2014, Istanbul
European Monitoring Center
for
Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
collect information
Information
Internet sources
Data bases
Scientific literature
Confis-cated
material
Meetings /
Conferences Patents
European Monitoring Center
for
Emerging Doping Agents
collect information / identify relevant developments collect information / identify relevant developments / question & summarize
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
European Monitoring Center
for
Emerging Doping Agents
other monitoring centers
laboratories
industry
police / customs control
scientific community
Contact
Collaboration
report mandate
Expert groups
e.g. WADA List Expert Group
review / evaluate / initiate actions
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Literature
European Monitoring Center
for
Emerging Doping Agents
other monitoring centers
laboratories
industry
police / customs control
scientific community
Contact
Collaboration
report mandate
Expert groups
e.g. WADA List Expert Group
review / evaluate / initiate actions
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Databases
European Monitoring Center
for
Emerging Doping Agents
other monitoring centers
laboratories
industry
police / customs control
scientific community
Contact
Collaboration
report mandate
Expert groups
e.g. WADA List Expert Group
review / evaluate / initiate actions
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
April 8, 2011
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
April 18, 2011
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Conferences
European Monitoring Center
for
Emerging Doping Agents
other monitoring centers
laboratories
industry
police / customs control
scientific community
Contact
Collaboration
report mandate
Expert groups
e.g. WADA List Expert Group
review / evaluate / initiate actions
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
May 28, 2006
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
February 2009
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
May 2011, Test methods
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
(2 x 20 kDa)
March 2012, FDA Approval
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
March 2013, FDA Recall
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Confiscations
European Monitoring Center
for
Emerging Doping Agents
other monitoring centers
laboratories
industry
police / customs control
scientific community
Contact
Collaboration
report mandate
Expert groups
e.g. WADA List Expert Group
review / evaluate / initiate actions
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
(home-made)
testosterone gel dispenser
100 160 220 280 340 400
85.0656
[C5H9O]+
257.1660
[C16H21N2O]+
316.2155
[C19H28N2O2]+
400.2740
[C24H36N2O3]+
modified furazabol conjugate
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Internet sources
European Monitoring Center
for
Emerging Doping Agents
other monitoring centers
laboratories
industry
police / customs control
scientific community
Contact
Collaboration
report mandate
Expert groups
e.g. WADA List Expert Group
review / evaluate / initiate actions
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Welcome!
We are the leading provider of SARMS S-4 in the United States.
At www.XXXXXXXXX.com we work very closely with our customers
to provide the very best products available on the market.
Our business is built on a philosophy of strong customer focus,
regular communication with a high degree of quality and value.
November 2005
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
CF3 N
H
O
OH CH3
O NH
O2N
O
CH3
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Patents
European Monitoring Center
for
Emerging Doping Agents
other monitoring centers
laboratories
industry
police / customs control
scientific community
Contact
Collaboration
report mandate
Expert groups
e.g. WADA List Expert Group
review / evaluate / initiate actions
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
GW501516 positives confirmed, three out of 4 riders are from the same BCR Pizza Hut team By Shane Stokes @ 3:10 PM Monday, April 15, 2013
UCI confirms Vargas Barrantes, Mudarra Segura, Morales Castillo,
Villalobos Azofeifa, and Miguel Ubeto provisionally suspended
Sergej Lisin arguably first athlete tested positive with GW1516 (2012)
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Science and Technology
February 8, 2014
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
The amended 2014 Prohibited
List (Section S2.), which now
includes Hypoxia-Inducible Factor
(HIF) activators Xenon and Argon,
will be in force as of September 1.
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
European Monitoring Center
for
Emerging Doping Agents
other monitoring centers
laboratories
industry
police / customs control
scientific community
Contact
Collaboration
report mandate
Expert groups
e.g. WADA List Expert Group
review / evaluate / initiate actions
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Confiscated Enobosarm
- Seized 2012 by German Customs Control
- 1 kg of pure Enobosarm (MK-2866)
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
130 kDa 100 kDa
70 kDa 55 kDa
40 kDa
35 kDa
25 kDa
150 kDa
15 kDa
10 kDa
SDS-PAGE:
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
17934 17936 17938 17940 17942 17944 17946 17948 17950 17952
m/z
0
10
20
30
40
50
60
70
80
90
100
Rel
ativ
e A
bu
nd
ance
[%
]
17942.8717941.88
17943.88
17940.88
17944.89
17939.85
17945.88
17938.88
17946.89
17937.86 17947.87
17948.9017936.8317949.86
17935.8517952.8617950.88
17934.85 17951.96
Unknown black market sample
Monoisotopic mass of the intact protein: 17931.84 Da
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
pET-32 Ek/LIC Vektor (Novagen):
• Hypothetical peptide including protein tags and MCS:
→ 183 AS, 19.5 kDa
Black market product = „empty“ vector
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
European Monitoring Center
for
Emerging Doping Agents
other monitoring centers
laboratories
industry
police / customs control
scientific community
Contact
Collaboration
report mandate
Expert groups
e.g. WADA List Expert Group
review / evaluate / initiate actions
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
World Anti-Doping Agency
Council of Europe (Advisory Group on Science)
World Association of Anti Doping Scientists
DFG Senate Commission on Food Safety
Network of (European) NADOs
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Industry partners
Center for Preventive Doping Research
Institute of Biochemistry
European Monitoring Center for Emerging Doping Agents
Acknowledgments
• German Federal Ministry of the Interior
• World Anti-Doping Agency