instruction manual mco-19aic-pe

Please read these instructions carefully before using this product, and save this manual for future use.

See page 66 for all Model Nos.

MCO-19AIC MCO-19AICUV MCO-19AICUVH Series

MCO-19AIC

MCO-19AIC(UV)

MCO-19AIC(UVH)

CO2 Incubator

Operating Instructions

1

CONTENTS

INTRODUCTION P. 2

PRECAUTIONS FOR SAFE OPERATION P. 3

LABELS ON INCUBATORS P. 7

ENVIRONMENTAL CONDITIONS P. 7

INCUBATOR COMPONENTS P. 8

Control panel and keypad P. 10

Remote alarm terminal P. 11

INSTALLATION SITE P. 12

INSTALLATION P. 13

Connecting a CO2 gas cylinder P. 14

PREVENTING CONTAMINATION P. 15

PRECAUTIONS FOR CULTURES P. 16

Using unlock key P. 16

INITIAL CLEANING METHOD P. 17

REMOVING INNER ATTACHMENTS P. 18

INSTALLING INNER ATTACHMENTS P. 20

FILLING HUMIDIFYING PAN P. 21

WATER LEVEL SENSOR P. 22

ROUTINE MAINTENANCE P. 23

CORRECT OPERATION P. 23

LCD PANEL P. 24

BASIC OPERATIONS ON CONTROL PANEL P. 25

BASIC PARAMETERS

Setting chamber temperature and CO2 density P. 26

Setting key lock P. 27

Removing key lock P. 27

Setting key lock password P. 28

Setting high limit alarm temperature P. 30

ALARM PARAMETERS P. 31

UV LAMP PARAMETERS

Using UV lamp P. 32

Precautions when using UV lamp P. 33

Setting UV lamp ON period P. 34

Lighting UV lamp for 24 hours P. 35

OTHER PARAMETERS

Setting date, time, and log interval P. 36

Initial settings (LCD/DAQ parameters) P. 38

DISPLAYING THE LOG P. 39

Transferring data P. 40

H2O2 DECONTAMINATION P. 41

Precautions when handling H2O2 reagent P. 45

ALARMS, SAFETY, AND SELF-DIAGNOSIS P. 46

CALIBRATION

Temperature/CO2 calibration P. 49

TROUBLESHOOTING P. 51

DISPOSAL OF UNIT P. 53

AUTOMATIC CO2 CYLINDER CHANGEOVER P. 58

AUTOMATIC CO2 DENSITY CALIBRATION P. 60

STACKING INCUBATORS P. 63

SPECIFICATIONS P. 65

PERFORMANCE P. 66

SAFETY CHECK SHEET P. 67

2

INTRODUCTION

■ Read this operating instruction carefully before using the Product and follow the instructions for safety

operation.

■ Our company disavows any responsibility for safety if the Product is used for other than the intended

use or used with any procedures other than those given in this operating instruction.

■ Keep this operating instruction in a suitable place so that it can be referred to as necessary.

■ The contents of this operating instruction are subject to change without notice for improvement of

performance or functions.

■ Contact our sales representative or agent if any page of the operating instruction is lost or the page

order is incorrect.

■ Contact our sales representative or agent if any point in this operating instruction is unclear or if there

are any inaccuracies.

■ No part of this operating instruction may be reproduced in any form without the expressed written

permission of our company.

CAUTION

Our company guarantees the product under certain warranty conditions. Our company in no way shall be responsible for any loss of content or damage of content.

Microsoft, Windows, Windows 7, Windows Vista, Windows XP and Windows 2000 are registered

trademarks in the United States of Microsoft Corporation and various other countries.

3

PRECAUTIONS FOR SAFE OPERATION

It is imperative that the user complies with this operating instruction as it contains important safety advice.

Items and procedures are described so that you can use this unit correctly and safely. If the precautions advised are followed, this will prevent possible injury to the user and any other person. Precautions are illustrated in the following way:

WARNING

Failure to observe WARNING signs could result in a hazard to personnel possibly resulting in serious injury or death.

CAUTION

Failure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property. Symbol shows; this symbol means caution. this symbol means an action is prohibited. this symbol means an instruction must be followed. Be sure to keep this operating instruction in a place accessible to users of this unit.

< Label on the unit > This mark is labeled on the cover in which the electrical components of high voltage are enclosed to prevent the electric shock. The cover should be removed by a qualified engineer or a service personnel only.

WARNING

As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity of the equipment. It is important that you assess the work site to ensure there is suitable and sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a safe environment must be considered. These may include atmosphere monitoring and warning devices.

4


Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to

rain water.

Only qualified engineers or service personnel should install the unit. The installation by

unqualified personnel may cause electric shock or fire.

Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from

turning over. If the floor is not strong enough or the installation site is not adequate, this may result

in injury from the unit falling or tipping over.

Never install the unit in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

Never install the unit in a flammable or volatile location. This may cause explosion or fire.

Never install the unit where acid or corrosive gases are present as current leakage or electric

shock may result due to corrosion.

Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not

grounded, it will be necessary to install a ground by qualified engineers.

Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

Connect the unit to a power source as indicated on the rating label attached to the unit. Use

of any other voltage or frequency other than that on the rating label may cause fire or electric shock.

Never store volatile or flammable substances in this unit if the container cannot be sealed. These

may cause explosion or fire.

Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.

This may cause electric shock or injury by accidental contact with moving parts. Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use

may cause bad effect on your health or environment.

Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any

repair or maintenance of the unit in order to prevent electric shock or injury.

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet

hand. This may cause electric shock.

WARNING

5

Ensure you do not inhale or consume medication or aerosols from around the unit at the time of

maintenance. These may be harmful to your health.

Never splash water directly onto the unit as this may cause electric shock or short circuit.

Never put containers with liquid on the unit as this may cause electric shock or short circuit when

the liquid is spilled.

Never bind, process, or step on the power supply cord, or never damage or break the power

supply plug. A broken supply cord or plug may cause fire or electric shock.

Do not use the supply cord if its plug is loose. Such supply cord may cause fire or electric shock.

Never disassemble, repair, or modify the unit yourself. Any such work carried out by an

unauthorized person may result in fire, or electric shock or injury due to a malfunction.

Disconnect the power supply plug if there is something wrong with the unit. Continued

abnormal operation may cause electric shock or fire.

When removing the plug from the power supply outlet, grip the power supply plug, not the cord.

Pulling the cord may result in electric shock or fire by short circuit.

Disconnect the power supply plug before moving the unit. Take care not to damage the power

cord. A damaged cord may cause electric shock or fire. Disconnect the power plug when the unit is not used for long periods. Keeping the connection

may cause electric shock, current leakage, or fire due to the deterioration of insulation. If the unit is to be stored unused in an unsupervised area for an extended period, ensure that

children do not have access and that doors cannot be closed completely.

The disposal of the unit should be accomplished by appropriate personnel. Remove doors to

prevent accidents such as suffocation.

Do not put the packing plastic bag within reach of children as suffocation may result.

Use the reagent specified by our company for H2O2 decontamination. Using a different H2O2

solution may result in explosion or damage to the incubator.

When performing H2O2 decontamination, securely close the internal and external doors. Failure to

do so may be harmful to health due to leakage of H2O2 gas.

During H2O2 decontamination, plug the access hole with the silicon cap that is provided. Failure

to do so may be harmful to health due to leakage of H2O2 gas.

WARNING

6


This unit must be plugged into a dedicated circuit protected by branch circuit breaker.

Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap

may cause fire resulting from abnormal heating.

Never store corrosive substances such as acid or alkali in this unit if the container cannot be

sealed. These may cause corrosion of inner components or electric parts.

Check the setting when starting up of operation after power failure or turning off of power

switch. The stored items may be damaged due to the change of setting.

Be careful not to tip over the unit during movement to prevent damage or injury.

Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for

the safety of service personnel.

Wear rubber gloves when handling the H2O2 reagent. Direct contact with the H2O2 reagent may

result in inflammation of the skin.

H2O2 decontamination can be performed only for the chamber and chamber attachments with

standard specifications, and not for any other objects.

Perform H2O2 decontamination with the chamber attachments arranged as specified by our

company. Arranging them in a different way may result in insufficient decontamination.

After H2O2 decontamination has been completed, wear rubber gloves and use a non-woven cloth

to wipe off the residual H2O2 fluid from the bottom of the chamber, any objects that were

decontaminated, and the bottoms of ducts.

CAUTION

7

LABELS ON INCUBATOR

Warning and caution labels are attached to the incubator. The following table describes the labels.

This label is attached to covers that access high-voltage electrical components to prevent electric shock. Only a qualified engineer or service personnel should be allowed to open these covers.

This symbol indicates an ultraviolet light (UV) caution.

This symbol indicates that caution is required. Refer to product documentation for

details.

This symbol indicates a hot surface.

This symbol indicates an earth.

This symbol means “ON” for a power switch.

This symbol means “OFF” for a power switch.

ENVIRONMENTAL CONDITIONS

This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):

■ Indoor use;

■ Altitude up to 2000 m;

■ Ambient temperature 5 oC to 40 oC

■ Maximum relative humidity 80 % for temperature up to 31 oC decreasing linearly to 50 % relative

humidity at 40 oC;

■ Mains supply voltage fluctuations not to exceed ±10 % of the nominal voltage;

■ Other supply voltage fluctuations as stated by the manufacturer;

■ Transient overvoltages according to Installation Categories (Overvoltage Categories) II; For mains

supply the minimum and normal category is II;

■ Pollution degree 2 in accordance with IEC 60664.

8

INCUBATOR COMPONENTS

1

2

3

4

5 6 7 89 (inside)

10

12

15, 16 (inside)

13 (inside)

17

Rear Right Side

11

18

20

Rear Left Side

For MCO-19AIC(UVH)/19AIC(UV) orwhen MCO-19UVS is installed.

19

21

22

14

For MCO-19AIC(UVH)/19AIC(UV) or when MCO-19UVS is installed.

Handles

Handles

9

1. Outer door: The outer door is held to the frame with the magnetic seal. The door heater is installed in

the door panel. The door opening is reversible. Contact our sales representative or agent to change the

door hinge from left to right or vice versa.

2. Inner door: The inner door is made of tempered glass. However do not subject the glass to excessive

impacts.

3. Leveling feet: The leveling feet can be turned to adjust the height. Adjust the feet so that the incubator

is level.

4. Trays: The trays can be pulled out toward you.

5. Tray supports: The tray supports can be removed by lifting the front side and pulling toward you.

6. Side supports: The right and left side supports can be removed for disinfection. Refer to page 19 and

23.

7. Fan cover: The fan cover serves as the inlet for circulating air. It is removable.

8. Duct: The duct for the path for circulating air. It is removable.

9. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an autoclave.

10. Sample air outlet: The sample air outlet also functions as an internal gas outlet. Normally, cover this

outlet with the sample air outlet cap.

11. Connecting port A/B for CO2 gas pipe: When the optional MCO-21GC gas auto changer is installed,

both ports A and B are available. If the MCO-21GC is not installed, only port A is available. Refer to page 14 for gas cylinder connection. Ensure that the gas pressure is set at 0.03 MPa(G) (0.3 kgf/cm2(G), 4.3

psi(G)). Refer to page 58 for gas auto changer.

12. Door switch: Detects the door opening/closing and stops the fan and electromagnetic valve for CO2

when the door is open. The UV lamp* is also deactivated by the door opening*.

13. Humidifying pan: Fill the humidifying pan with sterile distilled water. Install the humidifying pan

properly so that it can be covered with the humidifying pan cover.

14. Humidifying pan cover: The cover prevents the UV light entering the chamber. When filling the

humidifying pan, lift the front side and take out the humidifying pan. Refer to page 21 for details.

15. UV lamp*: This UV lamp does not generate ozone. Never look directly at the UV light. For

replacement, contact our sales representative or agent.

16. Water level sensor: This sensor detects the water level in the humidifying pan. Refer to page 22 for

details.

17. Remote alarm terminals: Refer to page 11.

18. Access port: Place the silicon caps on the port both outside and inside when the port is not being

used.

19. Power switch: This is the main switch for the incubator. It also functions as an overcurrent breaker.

20. Glow starter*: The glow is started if for the UV lamp.

21. Attachment location for electric key: This is the attachment location for the electric key included in

the optional component H2O2 decon set (MCO-HL). This kit must be attached to perform H2O2

decontamination. Refer to the installation procedure for MCO-HL for details.

22. Sample air outlet cap: Always attach this cap except when using the sample air outlet.

* MCO-19AIC(UVH)/19AIC(UV) or when an optional UV system set (MCO-19UVS) is installed.

10

INCUBATOR COMPONENTS

Control panel and keypad

1. LCD panel

2. High limit regulator:

This regulator is used to set the high

temperature limit.

3. Overheat indicator (OVER HEAT):

This indicator lights when the chamber

temperature reaches the high limit.

4. Menu key (MENU):

Press this key to access the menu.

5. Contrast knob (CONT.):

Turn this knob to adjust the contrast of the

LCD.

6. Buzzer key (BUZZER):

Press this key to silence the buzzer.

Note: It is not possible to silence the buzzer

for the high limit temperature alarm.

7. CE Key (CE):

Press this key to clear the entered value

when entering a setting.

8. Cursor Keys (Up, Down, Left, and Right):

Use these keys to move the cursor on the

LCD panel.

9. Enter key (ENT):

Press this key to select a menu command.

When entering a setting, pressing this key to

move to the next parameter.

10. Numeric Keys

11. H2O2 Key (H2O2):

Press this key to start the H2O2

decontamination. Refer to page 41-44 for

details.

※ The following optional accessories must be installed to perform decontamination.

・UV system set (MCO-19UVS)

(This is provided as standard equipment for the MCO-19AIC(UVH)/19AIC(UV).)

・H2O2 decon set (MCO-HL)

・H2O2 generator (MCO-HP)

1

2 4

5

6 7

8

9

10

11

3

11

Remote alarm terminal

The remote alarm terminal is located at the rear right side of the incubator.

The alarm is outputted from this terminal. Contact capacity is DC 30 V, 2 A.

Normal: Open

Alarm: Closed

Note:

• When the power switch is OFF or power is interrupted, the contact output will be closed.

• The remote alarm cannot be silenced by pressing the buzzer key (BUZZER) since the remote alarm is

not conjunct with the buzzer key (BUZZER).

Remote alarm terminal

REMOTE ALARM

MAX DC30V 2A

N.O.

Remote alarm

terminals

12

INSTALLATION SITE For correct operation of the incubator, install it in a location with the following conditions.

WARNING When using CO2 gas for control, make sure that there is adequate ventilation. Using CO2 gas in a small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition, when opening the incubator doors, do not directly inhale the air in the chamber.

Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO2 de l’air peut s’élever et peut être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la porte.

Normal air environment Install the incubator in an environment with normal air. Do not expose to direct sunlight Do not install the incubator in a location where it will be exposed to direct sunlight. If the incubator is operated in direct sunlight, performance will be adversely affected. Separate from heat sources Do not install the incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves. Heat will adversely affect the performance of the incubator. Ambient temperature at least 5 C lower than set temperature The control temperature of the incubator is at least 5 C higher than the ambient temperature. For example, if the chamber is controlled at 37 C, the ambient temperature must normally be no more than 32 C. Do not allow the ambient temperature to become too high. Strong and level floor Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the incubator will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure that the installation is stable. An unstable surface may result in vibration or noise.

WARNING Install the incubator at a location that can support the weight. If the floor is not strong enough or if the

installation is insufficient, the incubator may fall over and cause injury. Always make sure that the floor is strong, even, and level, and that the incubator will not tip over.

An insufficient installation may result in injury due to water leakage or the incubator falling over.

Low humidity Select a site with a relative humidity of 80 %R.H. or lower. Using the incubator in high humidity may result in current leakage or electric shock.

WARNING Do not use the incubator outdoors. If the incubator is exposed to rain water, it may result in current

leakage or electric shock.

Never install the incubator in a moist location, such as near a sink or water line, or where it is

likely to be exposed to water. In addition, do not install it near water or steam pipes. Moisture can

cause the insulation to deteriorate, which may result in current leakage or electric shock.

No inflammable or corrosive gas Never install the incubator in a location where it will be exposed to inflammable or corrosive gas. Doing so may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective casing, resulting in current leakage or electric shock. No falling objects Do not install the incubator in a location where there is the possibility of objects falling from above. Doing so may result in damage or accident.

13

INSTALLATION 1. Remove the packing tape and clean up.

Remove all the tape that is securing the doors and the inner attachments. Open the doors for ventilation.

If the outer panels are dirty, dampen a cloth with a diluted neutral detergent and wipe them. (Using an

undiluted solution may damage the plastic. Follow the diluting instructions for the detergent that is used.)

Wipe off the residual detergent with a wet cloth and then wipe off any moisture.

Note: Remove the cable tie banding the power supply cord. Prolonged banding may cause the corrosion of

the cord coating.

WARNING Do not leave the plastic wrapping bags within reach of children. If the bag is placed over a child’s

head, it can block the mouth and nose and cause suffocation.

2. Adjust the leveling feet.

Adjust the leveling feet by turning them counterclockwise to level

the incubator. (See the illustration on the right.)

3. Ground the incubator.

Ground the incubator during installation to prevent electric shock

in case the insulation is not sufficient. If there is no ground wire at

the location, consult with qualified service personnel.

When a ground must be installed

If a grounded 3-pole outlet is not available, then a ground must

be installed. Consult with qualified service personnel.

WARNING To prevent electric shock, always ground the incubator. If grounding is not possible, then have a ground

installed by qualified personnel. If the incubator is not grounded, it may result in electric shock.

Never connect the ground wire to a gas pipe, water pipe, lightning rod, or telephone ground wire.

Doing so may cause electric shock.

Installing a ground fault circuit breaker

If using the incubator in the location with moisture or humidity cannot be avoided, then it is recommended

that a ground fault circuit breaker be installed in the power supply circuit (i.e., the power supply at the

incubator). Have the circuit breaker installed by qualified service personnel.

CAUTION Do not climb on the incubator or place objects on top of it. Doing so may damage it or cause it to fall

over, resulting in injury. If it is to be stacked, refer to page 63 and stack it securely.

When the incubator is not in use

Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the

chamber is completely dry before closing the doors. Failure to do so may result in damage.

Before moving the incubator

Before moving the incubator, empty the water from the humidifying pan, disconnect the power supply plug

from the outlet, and make sure that the cord will not be damaged. Failure to do so may result in electric

shock or fire.

Leveling feet

14

INSTALLATION

Connecting a CO2 gas cylinder

WARNING When connecting a gas cylinder to the incubator, confirm the gas type. Confirm that the connections

are secure and that no gas will escape. Be sure to use the specified pressure. Using an incorrect

gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to escaping

gas.

Install the incubator in a location with adequate ventilation. If adequate ventilation cannot be

provided, then install an alarm system using CO2 and O2 densitometers.

1. Use a liquefied CO2 gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used.

2. Install a gas regulator rated at 25 MPa(G) (250 kgf/cm2(G), 3600 psi(G)) for the primary side, and 0.2

MPa(G) (2 kgf/cm2(G), 30 psi(G)) for the secondary side.

3. Using the gas tube that is provided, connect the gas regulator to the connecting port A/B for CO2 gas

pipe at the rear left side of the incubator.

Note:

If the CO2 gas is supplied to multiple CO2 incubators from a single gas cylinder, a CO2 solid will be

formed in the gas regulator. The gas regulator safety valve will operate, and there may an explosive

sound.

When the MCO-21GC is not mounted

Using the gas tube that is provided, connect the gas regulator for the CO2 gas cylinder to connecting port

A for CO2 gas pipe located at the rear side of the incubator. After connecting the gas tube, make sure that

no gas is leaking.

When the MCO-21GC is mounted

Connect a pair of CO2 gas cylinders with gas regulators independently. The gas supply line will be

switched automatically. Connect the main cylinder to the connecting port A for CO2 gas pipe and the

reserve cylinder to the connecting port B for CO2 gas pipe. After connecting the cylinders, make sure that

no gas is leaking.

For details on installing the optional automatic CO2 cylinder changeover kit (MCO-21GC), refer to the

MCO-21GC installation guide. For details on using the MCO-21GC, refer to page 58.

4. Set the CO2 gas on the secondary side to 0.03 MPa(G) (0.3 kgf/cm2(G), 4.3 psi(G)) for gas injection. As

the pressure increases, the CO2 gas density control range will increase. Excessive pressure may cause

gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen

deprivation due to the escaping of gas. If gas lines come loose, the incubator must be repaired.

The gas lines connected to the incubator will degrade over time. If any deterioration or abnormalities

are found during inspection, replace the lines immediately.

15

PREVENTING CONTAMINATION

To prevent contamination of the chamber, select a suitable installation site.

Avoid locations with high temperatures or humidity.

Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in

the air.

Avoid locations with passers-by or drafts.

Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry

of microorganisms into the chamber.

If possible, use a cleanroom.

To achieve a better culture, it is recommended that a cleanroom be used if one is available.

Use clean containers.

The greatest cause of contamination is dirty containers for cultures. Be careful not to get containers or

trays dirty when taking them in and out.

Keep the chamber clean.

Wipe off any fingerprints. If water spills from the humidifying pan, or if the doors are left open for a long

time, condensation may form on the inside of the doors. If that occurs, wipe off the condensation with a

dry sterile gauze. In particular, clean and disinfect the chamber if the culture medium is spilled. For details,

refer to “ROUTINE MAINTENANCE” on page 23.

Use sterile distilled water in the humidifying pan.

Always use sterile distilled water in the humidifying pan. Do not use ultrapure water, because it may

cause red rust-like particles in the humidifying pan.

The water level alarm is displayed in the status display area on the control panel. Quickly refill the water

in the humidifying pan when the water level alarm is displayed. Adding low-temperature water will

significantly lower the temperature in the chamber. Clean the humidifying pan once a month.

Keep the incubator out of direct airflows from air conditioners or fans.

Cool airflow from an air conditioner may cause condensation and lead to possible contamination.

16

PRECAUTIONS FOR CULTURES Leave space between culture containers. Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate spacing may result in uneven temperature distribution and CO2 gas density. Do not place harmful materials in the chamber. Never place samples that release acidic, alkali, or corrosive gas in the chamber. Doing so may cause damage resulting from discoloration or corrosion. Close the inner door. Always close the inner door before closing the outer door. Failure to close the inner door will adversely affect performance even if the outer door is closed. Open and close the doors gently. Always open and close the doors gently. Closing the doors forcefully may cause spillage of the culture medium, incomplete closing, or damage to the gasket. Before opening the inner door, check through the glass to confirm that the UV lamp is OFF (if the MCO-19AIC(UVH)/19AIC(UV) or the optional MCO-19UVS is installed). Be careful when closing the outer door. Use the handle when closing the outer door. Holding the door in other places may cause injury by getting fingers caught in the door. Do not lean on the outer door. Doing so may result in injury from the outer door coming loose or the incubator falling over, or it may cause current leakage or electric shock. Be careful of the inside of the outer door. The inside of the outer door may become hot. Avoid using excessive force on the inner door. Do not put your hand on the glass, poke it with sharp objects, or apply strong force. Doing so may result in injury from breaking the glass. Check the cause of any alarm buzzer. If an alarm buzzer sounds while the incubator is in use, immediately check the cause of the alarm. For details on what may cause an alarm buzzer to sound, refer to page 46.

Using unlock key

Unlocking when power is interrupted

If power is interrupted to the MCO-19AIC(UVH) or MCO-19AIC(UV)/19AIC with an MCO-HL installed, the

outer door is electrically locked. To unlock the outer door while the power is interrupted, use the unlock

key that is provided. To lock the outer door again, turn the unlock key to the lock direction while the outer

door is open. After the outer door has been locked condition manually, then close the outer door.

Note: The outer door cannot be locked by using the unlock key while the outer door is closed. Lock the outer

door while it is open. Attempting to turn the unlock key while the outer door is closed may damage the

electric lock system.

WARNING For the MCO-19AIC(UVH) or MCO-19AIC(UV)/19AIC with an MCO-HL installed, the outer door is

electrically locked during decontamination. The chamber is filled with H2O2 gas during decontamination.

Do not unlock the outer door during decontamination.

17

INITIAL CLEANING METHOD

Before using the incubator for the first time, clean dirt (tape residue, smear, etc.) from the chamber and the inner attachments thoroughly. To keep the chamber clean is essential to get the proper performance out of the incubator. Use the following steps to clean the incubator properly.

1. Remove the inner attachments, referring to “REMOVING INNER ATTACHMENTS” on page 18.

2. Clean the removed inner attachments, the chamber inside walls and the inner door gaskets with a cloth

or sponge soaked in neutral detergent, diluted by 5 % or less. (Undiluted detergent can damage the

plastic components. For the dilution, refer to the instruction of the detergent.) (Fig. 1)

CAUTION Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause

discoloration, corrosion, or rusting.

Be careful to keep the diluted detergent or water out of the temperature sensor, the CO2 gas injection port,

the inner sample air access port, the fan motor shaft bearing, and the inner sample air outlet

(Fig.2 ). Also, do not wash the temperature sensor, the water level sensor and the UV lamp using

detergent. Doing so may cause failure. (Fig. 2)

3. Soak a gauze or unwoven cloth in distilled water and wring it tightly, and then wipe off the residual

detergent thoroughly.

4. Wash the silicon caps (2 pcs) for the access port and the fan using the above mentioned detergent and

rinse them with distilled water, and then autoclave them for sterilization (121 oC, 20 minutes).

5. Wipe up the inside walls and the inner attachments like trays thoroughly with a cloth or unwoven cloth

soaked in alcohol for disinfection. Be careful not to leave any residue alcohol.

6. Reinstall the inner attachments correctly and securely, referring to “INSTALLING INNER

ATTACHMENTS” on page 20.

Fig. 1 Fig. 2

(UV lamp)

Temp. sensor

Water level sensor

Silicon cap

18

REMOVING INNER ATTACHMENTS

CAUTION Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may

result in cuts or abrasions from sharp edges or corners.

Be careful not to damage the water level sensor or the UV lamp in the duct (if the

MCO-19AIC(UVH)/19AIC(UV) or the optional MCO-19UVS is installed).

Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause

discoloration, corrosion, or rusting.

1. Turn OFF the power to the incubator.

2. Open the outer and inner doors and pull out all the trays. (Fig. 1)

3. Lift the front of the tray supports and pull them out. (Fig. 2)

4. Lift the humidifying pan cover off from the pins on the rear side. (Fig. 3)

5. Pull out the humidifying pan. (Fig. 4)

6. Loosen the two screws securing the fan cover and take off the fan cover. (Fig. 5)

7. Lift the duct and remove it from the pins on the rear side. (Fig. 6)

Fig. 1 Fig. 2 Fig. 3


Tray support

Humidifying pan cover

Pins

Humidifying pan

Fan cover

Duct

19

8. Remove the fan by pulling out the central spring and then by pulling out the fan. (Fig. 7)

9. Remove the screw securing the clamp for the side support. (Fig. 8)

10. Remove the clamp. (Fig. 9)

11. Lift the side supports off of the pins. (Fig. 10)

12. Remove the silicon caps of the access port, one each from interior (Fig. 11) and exterior (Fig. 12).

①Pull out the central spring

②Pull out the fan


Fig. 10 Fig. 11 Fig. 12

Screw Clamp

Side

support

Silicon cap Silicon cap

20

INSTALLING INNER ATTACHMENTS

Use the following steps to install the inner attachments properly.

1. To reinstall all the attachments, perform the procedure in reverse order from step 12 on page 19.

2. When installing the fan, insert it to the motor shaft securely. Lightly turn the fan manually to make sure

that it does not touch the rear panel. Then install the central spring. (Fig. 1)

CAUTION If the fan is not inserted deep enough, the intended velocity performance cannot be achieved, which may

cause culture failure or insufficient decontamination.

3. To install the duct, confirm 4 pins are securely installed in the 4 holes of the duct. (Fig. 2)

CAUTION If the duct is fixed insecurely, the intended velocity performance cannot be achieved, which may cause

culture failure or insufficient decontamination.

Fig. 1 Fig. 2

4. To install the side support, confirm the direction of fixing holes as shown in Fig. 3.

5. As shown in Fig. 4, set the tray with only the front edge bent down. If the tray is set in the wrong

direction, it may not be level and may become unstable.

Fig. 3

① Push the fan deeply.

② Install the central spring afterwards. ③ Lightly turn the fan manually to make

sure that it does not touch the rear panel.

Bent down Fig. 4

21

FILLING HUMIDIFYING PAN

Use the following procedure to fill the humidifying pan or to replace the water.

1. Lift the humidifying pan cover. (Fig. 1)

2. Pull out the humidifying pan toward you. (Fig. 2)

3. Dispose of the remaining water in the humidifying

pan and clean the humidifying pan with a diluted

detergent. Then rinse it thoroughly with distilled

water and wipe it with alcohol for disinfection.

4. Wipe all moisture from the bottom of the chamber.

5. Return the humidifying pan to the chamber and

pour sterile distilled water (approx. 1.5 L, preheated

to 37 C). (Fig. 3)

CAUTION Install the humidifying pan in a longitudinal direction

as its shorter side is placed in the back. Improper

installation may cause condensations.

6. Set the humidifying pan with the inner side flush

against the back, and replace the humidifying pan

cover. Close the inner door and the outer door, and

confirm that RH PAN is not displayed in reverse

video in the status display field.

Note:

• Preheat the water to 37 C to be poured into the

humidifying pan. Adding low-temperature water will

lower the temperature and humidity in the chamber.

• If the water level alarm indication “RH PAN” is

displayed, use the above procedure to replenish the

water.

CAUTION When refilling the water in the humidifying pan, always wipe off any dirt from the water level sensor

with alcohol for disinfection. While doing that, be careful not to apply excessive force to the sensor

lead wire.

Caution：

In order to adjust the humidity inside the incubator chamber optimally and prevent condensation on the

chamber surface and the inner door, it is designed to create the lower temperature area underneath the

humidifying pan to recondense the evaporated moisture in the humidifying pan. Although the

condensation may be seen round the humidifying pan on the bottom of chamber (inside the humidifying

pan cover), it is not a malfunction.

Fig. 1

Fig. 3

Fig. 2

22

WATER LEVEL SENSOR

This incubator is equipped with a water level sensor. The water level sensor is set automatically when the

humidifying pan is installed. Take care not to damage the water level sensor when removing or installing

the humidifying pan.

When the humidifying pan is removed (side view)

When the humidifying pan is installed (side view)

Note: Make sure that the humidifying pan is pushed all the way to the back and that the water level

sensor comes down when you install the humidifying pan.

Note:

• Lift the water level sensor before installing the humidifying pan if the water level sensor is in the lower

position after maintenance.

• When installing the humidifying pan, make sure that the humidifying pan is set properly and that water

level sensor comes down into the humidifying pan. “RH PAN” will be displayed in reverse video in the

status display field on LCD panel if the water level sensor does not come down completely. If necessary,

set the humidifying pan again in the proper location.

• The water level sensor detects the water level every 30 minutes and just after the outer door is closed. It

takes several seconds to detect the water level. Therefore, “RH PAN” may be displayed in reverse video

several times in the status display field on LCD panel after the outer door is closed even when the

humidifying pan is full.

CAUTION Foreign particles on the water surface can adhere to the water level sensor and fittings by capillary action

because the water level sensor is always in the water. The adhered foreign particles degrade water level

sensor performance and “RH PAN” may be displayed in reverse video in the status display field on LCD

panel even though there is sufficient water in the humidifying pan. Be sure to wipe OFF any dirt on the

water level sensor with alcohol for disinfection whenever you change the humidifying water. When

cleaning the water level sensor, take care not to apply excessive force to the lead wires.

Water level sensor Humidifying pan

23

ROUTINE MAINTENANCE

To use this unit in a clean condition, clean the chamber and all the inner attachments at least once a

month.

1. Remove all the inner attachments by the procedures shown on page 18.

2. Clean the chamber and all the inner attachments by the procedures shown on page 17.

3. Install all the inner attachments by the procedures shown on page 20.

● When there is excessive dirt, contact our sales representative or agent.

CORRECT OPERATION

Use the following procedure to start trial operation or actual operation of the incubator.

1. Install the incubator correctly, referring to “INSTALLATION” on page 13.

2. Remove the packing materials from the chamber and inner attachments. Clean and disinfect the

chamber and all the inner attachments, referring to above “ROUTINE MAINTENANCE”.

3. Add approximately 1.5 L of sterile distilled water to the humidifying pan. (Refer to page 21.)

4. Connect the power supply cord to the outlet. Turn ON the power switch on the left side of the rear panel

of the incubator.

Note:

The humidity in the incubator chamber is adjusted to the optimum setting. To prevent condensation on the

surface inside chamber and the inner door, there is a low-temperature area under the humidifying pan in

the bottom of chamber to recondense evaporated moisture. Condensation may occur around the

humidifying pan at the bottom of the chamber (on the inside of the humidifying pan cover), but this does

not indicate a problem.

24

LCD PANEL

The following display (called the “Top Display”) will appear when the power switch is turned ON. The

default temperature is 37.0 oC and the default CO2 density is 0 %. The date and time are preset at the

factory. Refer to page 36 to change the date and time.

1. Display of set value of temperature

The set value of chamber temperature is displayed.

2. Display of current temperature

The current chamber temperature is displayed.

3. Display of set value of CO2 density

The set value of the chamber CO2 density is displayed.

4. Display of current CO2 density

The current chamber CO2 density is displayed.

5. Display of current CO2 cylinder

A and B will be displayed if an optional gas auto changer (MCO-21GC) is installed. The port of the CO2

gas pipe that is currently supplying CO2 will be displayed in reverse video. Nothing will be displayed if the

MCO-21GC is not installed.

6. Status display field

Various status or alarms are displayed.

・When the UV lamp is lit: “UV” is displayed in reverse video. (If the MCO-19AIC(UVH)/19AIC(UV) or the

optional MCO-19UVS is installed).

・When the humidifying water is low: “RH PAN” is displayed in reverse video and blinks.

・When the door is open: “Door: Open” is displayed in reverse video.

7. Message display field

A message is displayed when fault occurs. The message is displayed alternately in normal characters

and reverse video. Refer to page 46-48 for alarm details. “OK” is displayed during normal operation.

0

1

0

2 1

1

1

2

1

3

1

4

1

5

1

6

1

7

1

8

1

9

2

0

2

1

2

2

2

3

2

4

2

5

2

6

2

7

2

8

2

9

3

0

3

1

3

2

3

3

3

4

3

5

3

6

3

7

3

8

3

9

4

0

1 T e m p 3 7 . 0 o C C O 2 5 . 0 % A B

2

3

4

5 S t a t u s U V R H P A N D o o r : O p e n

6 O K

7

３７.０５.０

5 1

2

6

3

4

7

25

BASIC OPERATIONS ON CONTROL PANEL

The following operations are possible through the control panel:

Setting the temperature・・・The chamber temperature can be set (page 26).

Setting the CO2 density・・・The chamber CO2 density can be set (page 26).

Setting the key lock・・・Changing the temperature and CO2 density can be disabled (page 27).

Setting alarms・・・The temperature alarm, the CO2 density alarm (page 31), and the high limit alarm

temperature (page 30) can be set.

Setting UV parameters・・・ON/OFF parameters and the ON period of the UV lamp can be set for the

MCO-19AIC(UVH)/19AIC(UV) or when the incubator is equipped with the optional MCO-19UVS (page

32).

Setting other parameters・・・The initial settings of the date, time, and the log cycle (page 36), and the

LCD display and baud rate (page 38) can be set.

Displaying the operation log and transferring data・・・A graph of past operation data can be

displayed (page 39) and operation data can be transferred to a PC (page 40).

H2O2 decontamination・・・H2O2 decontamination of the chamber and the inner attachments can be

performed when the optional H2O2 generator (MCO-HP) is installed (page 42).

Automatic CO2 cylinder changeover・・・The cylinder can be switched when the optional gas auto

changer (MCO-21GC) is installed (page 58).

Setting the CO2 standard gas density・・・The CO2 standard gas density can be set when the optional

STD gas auto calibration kit (MCO-SG) is installed. (page 60)

26

BASIC PARAMETERS

Setting chamber temperature and CO2 density

The setting procedure for the chamber temperature and the CO2 density are given below. (Default

settings: Chamber temperature: 37 oC, CO2 density: 0 %)

The incubator automatically starts operation using these settings after the power is turned ON.

1. From the “Top Display”, press the menu key (MENU) to access the menu. Select “Set” and press the

enter key (ENT).

2. The “Stand-by Setting” display will be displayed. Set the parameters.

The setting ranges of the parameters are as follows:

●Temperature: 0 oC to 50 oC. To 36.5 oC, enter 365.

●CO2 density: 0 % to 20 %. To set 5.0 %, enter 050.

3. Press the menu key (MENU) to display the menu after setting the parameters. Select “OK” and press

the enter key (ENT). The settings will be saved.

Note:

When starting the incubator for the first time or after not using it for an extended period of time, allow at

least 4 hours for the chamber temperature, the humidity, and the CO2 sensor to become stable after

setting the desired chamber temperature at a 0 % CO2 density. Then change the setting to the desired

CO2 density.

1 T e m p 3 7 . 0 o C C O 2 5 . 0 %

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０ M E N U

S e t

L o g

T o o l s

1 S t a n d - b y S e t t i n g

2 T e m p e r a t u r e 3 7 . 0 o C ( 0 . 0 o C - 5 0 . 0 o C )

3 C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % )

4

5 K e y L o c k 0 ( 0 . U n l o c k 1 . L o c k )

6 H i g h L i m i t 5 2 . 0 o C

M E N U

O K

C a n c e l

27

Setting key lock

1. To set the key lock, change the value of the key lock parameter from 0 to 1 on the “Stand-by Setting”

display and press the enter key (ENT). The buzzer will sound briefly and the keys will be locked.

2. “Key Lock” will be displayed in reverse video at the right side of “Stand-by Setting”, and changing the

temperature and CO2 density settings will be disabled.

Removing key lock

1. To remove the key lock, change the value of the key lock parameter from 1 to 0 on the “Stand-by

Setting” display and press the enter key (ENT).

2. The cursor will move to the Password field. Input the 4-digit password (default: 0000) and press the

enter key (ENT). When key lock is release, the buzzer will sound briefly, and “Key Lock” will disappear

from the “Stand-by Setting” display.

1 S t a n d - b y S e t t i n g K e y L o c k

2 T e m p e r a t u r e 3 7 . 0 o C ( 0 - 5 0 . 0 o C )

3 C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % )

4

K e y L o c k 0 P a s s w o r d

H i g h L i m i t 5 2 . 0 o C



3 C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % )

4


6 H i g h L i m i t 5 2 . 0 o C

M E N U

O K

C a n c e l

1 S t a n d - b y S e t t i n g K e y L o c k


3 C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % )

4


6 H i g h L i m i t 5 2 . 0 o C

28

BASIC PARAMETERS

3. Press the menu key (MENU) to display the menu, select “OK” and press the enter key (ENT). The

settings will be saved.

Note: The buzzer will sound for a long time if the wrong password is entered. Enter the correct password.

The password for releasing the key lock must be shared and administered among all users of the

incubator. The password is set to 0000 when the incubator is shipped from the factory. The procedure for

changing the password is given next.

Setting key lock password

1. From the “Top Display”, press the menu key (MENU) to display the menu, select “Tools”, and press the

enter key (ENT).

2. Select “Key Lock PW Setting” from the “Select Tools” display, press the menu key (MENU) to display

the menu, select “OK”, and press the enter key (ENT).

S e l e c t T o o l s ( 1 / 2 )

T e m p / C O 2 C a l i b r a t i o n

A l a r m S e t t i n g

L C D / D A Q S e t t i n g

U V S e t t i n g

K e y L o c k P W S e t t i n g

D a t e T i m e

M E N U

O K

S v C

C a n c e l

1 T e m p 3 7 . 0 o C C O 2 5 . 0 %

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０ M E N U

S e t

L o g

T o o l s

29

3. Input the current user password (4 digits), select “OK”, and press the enter key (ENT). (The default

user password is 0000 when the incubator is shipped from the factory.)

4. Enter the new user password (4 digits), select “OK”, and press the enter key (ENT).

5. Enter the new user password again, select “OK”, and press the enter key (ENT) to save. After that

press the menu key (MENU) to display the menu, and select “Cancel“ from the menu.

Note:

Be careful not to forget the key lock password. If you have forgotten the password and cannot release the

key lock, contact our sales representative or agent.


C u r r e n t U s e r P a s s w o r d ＊＊＊＊


N e w U s e r P a s s w o r d ＊＊＊＊

R e E n t e r U s e r P a s s w o r d ＊＊＊＊


N e w U s e r P a s s w o r d ＊＊＊＊

30

BASIC PARAMETERS

Setting high limit alarm temperature

The high limit temperature alarm is provided with the incubator. The alarm temperature can be changed

by using the following procedure.

1. In the “Stand-by Setting” display, turn the high limit regulator on the control panel using a small

screwdriver to set the desired high limit alarm temperature. The high limit temperature can be set

between 34 oC and 52 oC.

Note: ・Set the high limit alarm temperature to at least 5 oC higher than the chamber set temperature.

・Set the high limit alarm temperature to at least 50 oC when H2O2 decontamination is performed.

2. After setting the parameter, press the menu key (MENU) to display the menu, select “OK”, and press

the enter key (ENT). The alarm temperature will be saved.

Refer to the tables of “ALARMS, SAFETY, AND SELF-DIAGNOSIS” on page 46 for details.

Note: ・The high limit alarm temperature will be changed at any time by turning the high limit regulator even if

the “Stand-by Setting” display is not displayed.

・Set the high limit alarm temperature after the chamber reaches the set temperature for operation.



3 C O 2 D e n s i t y 5 . 0 % ( 0 . 0 % - 2 0 . 0 % )

4


6 H i g h L i m i t 5 2 . 0 o C

M E N U

O K

C a n c e l

31

ALARM PARAMETERS

1. Press the menu key (MENU) from the “Top Display” to display the menu, select “Tools”, and press the

enter key (ENT).

2. Select “Alarm Setting” from the “Select Tools” display, press the menu key (MENU) to display the menu,

select “OK”, and press the enter key (ENT).

3. The “Alarm Setting” display will appear. The temperature alarm, the CO2 alarm, the alarm delay, the

ring back, and the door delay can be set on this display. The alarm buzzer can be silenced by pressing

the buzzer key (BUZZER). The alarm buzzer will sound again after the specified ring back time if the

condition that caused the alarm continues. The ring back time can be set.

・Temp Alarm: ±1.0 oC to ±5.0 oC (Default: ±1.0 oC)

・CO2 Alarm: ±0.5 % to ±5.0 % (Default: ±1.0 %)

・Alarm Delay: 0 minute to 15 minutes (Default: 15 minutes)

・Ring Back: 1 minute to 99 minutes, or OFF (Default: 30 minutes)

・Door (alarm) Delay: 1 minute to 30 minutes (Default: 2 minutes)

S e l e c t T o o l s ( 1 / 2 )




U V S e t t i n g


D a t e T i m e

M E N U

O K

S v c

C a n c e l

1 A l a r m S e t t i n g

3 T e m p A l a r m ± 1 . 0 o C ( ± 1 . 0 ° C － ± 5 . 0 ° C ）

C O 2 A l a r m ± 0 . 5 % ( ± 0 . 5 % － ± 5 . 0 % ）

4

5 A l a r m D e l a y 1 5 m i n ( 0 - 1 5 m i n )

R i n g B a c k 3 0 m i n ( 0 . O F F 1 － 9 9 m i n )

6 D o o r D e l a y 2 m i n ( 1 - 3 0 m i n )

M E N U

O K

C a n c e l

1 T e m p 3 7 . 0 o C C O 2 5 . 0 %

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０ M E N U

S e t

L o g

T o o l s

32

UV LAMP PARAMETERS

The UV lamp is used with the MCO-19AIC(UVH)/19AIC(UV) or the MCO-19AIC with an MCO-19UVS UV

system set installed. Use the following procedures to make the settings.

Using UV lamp

The UV lamp is located inside the duct to sterilize the water in the humidifying pan and the air circulating

in the chamber. Observe the following points to use the UV lamp correctly.

When all the inner attachments are installed correctly, only the inside of the duct and the inside of the

humidifying pan cover are exposed to UV light.

Correctly install all of the inner attachments when starting a cell culture, and never turn ON the UV lamp

when the humidifying pan cover is removed.

Always install the humidifying pan cover even when using the incubator without turning ON the UV

lamp. Leaving the cover uninstalled will affect the chamber temperature distribution and the humidity

recovery performance.

The UV lamp stays lit for a preset period of time after the outer door is closed. The default setting is for

five minutes.

If the outer door is not opened for at least 12 consecutive hours, the UV lamp will light for the preset

time period every 12 hours.

The recommended replacement time for the UV lamp (i.e., when the UV output ratio drops to 60 % to

70 % of its initial value) is when the accumulated ON time reaches 1,000 hours. When the accumulated

ON time reaches approximately 1,000 hours, the “UV” will blink in the status display field on the LCD

panel (when the UV lamp is not lit) and “Warning :UV Life” is displayed in the message display field on the

LCD panel. It is recommended that the UV lamp be quickly replaced at this point. Contact our sales

representative or agent for information on replacing the UV lamp.

If the UV lamp burns out, “Err18:UV Lamp Abnormal” will be displayed in the message display field. If

this occurs, replace the UV lamp. When replacing the UV lamp, replace the glow starter (type FG-7P) at

the same time. Contact our sales representative or agent for information on replacing the UV lamp.

If the UV lamp burns out (“Err18:UV Lamp Abnormal” will be displayed in the message display field), it

will not be possible to perform H2O2 decontamination. Replace the UV lamp and the glow starter (type

FG-7P).

33

Precautions when using UV lamp

WARNING Do not look directly at UV light. UV light is harmful to the eyes.

Always use the humidifying pan and humidifying pan cover.

The humidifying pan and humidifying pan cover prevent UV light from escaping. Always use them even

when not humidifying. To check whether the UV lamp is lit, open the outer door and then press the door

switch with the inner door still closed. Visible blue light can be confirmed from the front of the humidifying

pan cover. UV light is harmful to the eyes, so do not light the UV lamp when the inner door or the

humidifying pan cover is open.

Be careful when handling the UV lamp.

There is a UV lamp inside of the duct. Be careful not to damage it when installing or removing the inner

attachments, the humidifying pan, or the H2O2 generator.

34

UV LAMP PARAMETERS

Setting UV lamp ON period

Use the following procedure to change the setting of the UV lamp ON period.


enter key (ENT).

2. Select “UV Setting” from the “Select Tools” display, press the menu key (MENU) to display the menu,


3. The “UV Setting” display will appear. Set the UV timer for the UV lamp ON period.

4. Press the menu key (MENU) to display the menu, select “OK”, and press the enter key (ENT). The

parameter will be saved.

1 T e m p 3 7 . 0 o C C O 2 5 . 0 %

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０ M E N U

S e t

L o g

T o o l s

S e l e c t T o o l s ( 1 / 2 )




U V S e t t i n g


D a t e T i m e

M E N U

O K

S v c

C a n c e l

1 U V S e t t i n g

3 U V T i m e r 5 m i n ( 0 - 3 0 m i n )

U V L i f e 0 %

4 U V T i m e r E x t . + 0 %

5

U V 2 4 h M o d e 0 ( 0 . O F F 1 . O N )

6

35

The UV timer can be set from 0 minute to 30 minutes. The default setting is for 5 minutes. When the UV

timer is set to zero, the UV lamp will not light.

If the outer door is opened while the UV lamp is lit, the lamp will turn OFF. Then, when the outer door is

closed, the lamp will light for the preset period of time.

If the UV lamp ON time is set for longer than 10 minutes or if only the outer door is repeated opened

and closed, condensation may occur in the chamber and may affect temperature distribution. It will also

shorten the service life of the UV lamp.

When replacing the UV lamp, contact our sales representative or agent.

Note: To compensate for the drop in UV ray output along with increased accumulated ON time of the UV

lamp, the incubator automatically extends the UV lamp ON time according to the accumulated ON time.

The automatically extended time is displayed as a percentage under “UV Timer Ext.” in the “UV Setting”

display.

Lighting UV lamp for 24 hours

If the chamber has been contaminated by dirt or by spilling the medium, use the following procedure to

decontaminate the chamber by lighting the UV lamp for 24 hours.

1. Remove all attachments from the chamber, including the trays, the tray supports, the side supports, the

fan cover, the duct, the fan, the humidifying pan, and the humidifying pan cover. Clean all the

attachments in an autoclave or with alcohol for disinfection.

2. Clean and wipe off inside the chamber with alcohol for disinfection.

3. Set the CO2 density to 0 % and set the UV 24-hour mode to 1 on the “UV Setting” display.

4. Press the menu key (MENU) to display the menu, select “OK”, and press the enter key (ENT). The

parameter will be saved.

・This procedure must be preformed only with the outer door closed and the UV lamp turned OFF.

・The UV lamp will light continuously for 24 hours after this parameter is set. The setting is canceled if the

outer door is opened. Perform the above procedure to set the UV 24-hour mode again if you open the

outer door. ・Install all attachments after completion of the UV 24-hour mode.

Note: Set the high limit alarm temperature to at least 10 oC higher than the chamber set temperature

when using the UV 24-hour mode. The UV 24-hour mode may cause the automatic set temperature

alarm because the temperature of the chamber will increase.

1 U V S e t t i n g

3 U V T i m e r 5 m i n ( 0 - 3 0 m i n )

U V L i f e 0 %

4 U V T i m e r E x t . + 0 %

5

U V 2 4 h M o d e 1 ( 0 . O F F 1 . O N )

6

36

OTHER PARAMETERS

Setting date, time, and log interval

1. Press the menu key (MENU) from the “Top Display” to display the menu, select “Tools” and press the

enter key (ENT).

2. Select “Date Time” from the “Select Tools” display, press the menu key (MENU) to display the menu,


1 T e m p 3 7 . 0 o C C O 2 5 . 0 %

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０ M E N U

S e t

L o g

T o o l s

S e l e c t T o o l s ( 1 / 2 )




U V S e t t i n g


D a t e T i m e

M E N U

O K

S v c

C a n c e l

37

3. The “Date Time” display will appear. Set the date, time and log interval.

・Entering the date

Example for March 1, 2009: Enter 090301 in the “Date Field”.

・Entering the time

Example for 10:05:00: Enter 100500 in the “Time Field”.

・Entering the log interval

Example for 6 minutes: Enter 6 in “Log Interval Field”.

Note:

■ The default is 6 minutes.

■ The log interval can be set to between 2 minutes and 30 minutes.

■ Relation between the log interval and the period that can be saved

1: Log interval of 2 minutes -- About 5 days



For the data beyond the above period, the older data is deleted to save the newer data.

1 D a t e T i m e

3

D a t e 0 9 / 0 3 / 0 1 ( Y Y / M M / D D )

4 T i m e 1 0 : 0 5 : 0 0 ( h h ： m m ： s s )

5

L o g I n t e r v a l 6 m i n ( 2 - 3 0 m i n )

6

M E N U

O K

C a n c e l

38

OTHER PARAMETERS

Initial settings (LCD/DAQ parameters)

1. Select “LCD/DAQ Setting” from the “Select Tools” display, press the menu key (MENU) to display the

menu, select “OK”, and press the enter key (ENT).

2. The “LCD/DAQ Setting” display will appear. Set the initial setting for each parameter as necessary.

・LCD Back Color: Setting of the background color (1: Blue, 2: White)

Note:

DAQ is an external monitoring system of the chamber status. It is necessary to set the DAQ speed, DAQ

ID, and DAQ mode to use the optional communications software. Communications software is an

optional accessory. Contact our sales representative or agent for details.

1 L C D / D A Q S e t t i n g

3 L C D B a c k C o l o r 1 ( 1 . B l u e 2 . W h i t e )

D A Q S p e e d 0 ( 0 . 2 4 2 . 9 6 3 . 3 5 0 )

4 D A Q I D 0 ( 0 . O F F 1 - 2 5 5 )

5 D A Q M o d e 0 ( 0 . L o c a l 1 . R e m o t e )

R e m o t e A l a r m 0 ( 0 . O F F 1 . A c t i v e )

6

M E N U

O K

C a n c e l

S e l e c t T o o l s ( 1 / 2 )




U V S e t t i n g


D a t e T i m e

M E N U

O K

S v c

C a n c e l

39

DISPLAYING THE LOG

A log of the past chamber temperatures and CO2 densities can be displayed on a graph.

1. Press the menu key (MENU) from the “Top Display” to display the menu, select “Log” and press the

enter key (ENT).

2. The log will be displayed with dots. Press the “Up Cursor key” and “Down Cursor key” to switch

between the temperature and CO2 density displays. Press the “Left Cursor key” and “Right Cursor key” to

scroll the displayed data (Left Cursor key: older data, Right Cursor key: newer data).

1 T e m p 3 7 . 0 o C C O 2 5 . 0 %

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０ M E N U

S e t

L o g

T o o l s

1 5 0 ° C 2 0 0 9 / 0 3 / 0 1 T e m p

3

4

5

6 0 1 2 2 4

Switched with Up and Down Cursor keys

1 2 0 % 2 0 0 9 / 0 3 / 0 1 C O 2

3

4

5

6 0 1 2 2 4

Temperature

CO2 density

40

DISPLAYING THE LOG

Transferring data

Use the following procedure to transfer the log data to a PC.

1. To transfer the log data for one day, press the menu key (MENU) to display the menu, select “PC 1D”,

and press the enter key (ENT). To transfer all of the log data, select “PC All” and press the enter key

(ENT).

2. A “Progress” display will appear. On a HyperTerminal on PC, specify a transfer, text capture, and the

name of the file to save. Use TXT or CSY as the file name extension. Press the menu key (MENU) to

display the menu, select “Start”, and press the enter key (ENT).

The transfer will be started. “Finished” will be displayed when the transfer has been completed. Select

“Cancel“ from the menu after the transfer has been completed and press the enter key (ENT).

Setting in PC side for transmission of log data (For Windows 2000 and Windows XP)

1. From the Windows Start Button, select Program - Accessories - Communications -

HyperTerminal to start the HyperTerminal. (If the HyperTerminal is not available from the Start Menu,

execute the following file: C:¥Program Files¥Windows NT¥hypertrm.exe.)

2. In the HyperTerminal Window, set a new connection, the name (for example, Matsushita), connection

settings, method of connection, COM1, properties of COM1, and port.

Baud rate: 9,600, Data bits: 8, Parity: None, Stop bits: 1, Flow control: Xon/Xoff.

(The communications condition of the incubator will be set automatically to the above settings when the

“Progress” display appears.)

Note: An optional interface board MTR-480 (RS-232C/RS-485) is required to transfer data.

1 P r o g r e s s

3

S e n d l o g d a t a t o P C .

4

5 L o g D a t a 2 0 0 9 / 0 3 / 0 1

6 F i n i s h e d .

M E N U

S t a r t

C a n c e l

1 5 0 ° C 2 0 0 9 / 0 3 / 0 1 T e m p

3

4

5

6 0 1 2 2 4

M E N U

P C 1 D

P C A l l

C l e a r

C a n c e l

41

H2O2 DECONTAMINATION

When the chamber has been contaminated, or when cleaning the chamber prior to starting a culture, H2O2 decontamination can be performed using the H2O2 generator. The following products must be purchased separately in order to perform H2O2 decontamination. Before performing H2O2 decontamination, make sure that these are correctly installed. UV system set (MCO-19UVS) (Not required for the MCO-19AIC(UVH)/19AIC(UV).) H2O2 decon set (MCO-HL) H2O2 generator (MCO-HP)

The H2O2 decontamination function decontaminates the chamber and the inner attachments. Do not place any objects other than the specified attachments in the chamber during decontamination.

Before performing H2O2 decontamination, clean the chamber and the inner attachments with alcohol for disinfection.

Before performing H2O2 decontamination, make sure that the duct, the fan and the H2O2 generator are securely attached. (Fig. 1 - 3 on page 52)

The chamber temperature during H2O2 decontamination reaches 45 C. Before performing H2O2 decontamination, set the high limit temperature alarm to 50 C or higher.

For the H2O2 reagent, use one bottle of the H2O2 reagent specified by our company (MCO-H2O2).

After decontamination, dilute the residual H2O2 reagent in the H2O2 generator with a large quantity of water and dispose of it, and rinse with distilled water. (Do not wash either the inside or outside of the H2O2 generator with alcohol.)

WARNING Use the reagent specified by our company for H2O2 decontamination. Using a different H2O2 solution may cause explosion or damage to the incubator, or insufficient decontamination. Do not use chemicals other than the H2O2 reagent, such as alcohol. Doing so may result in damage to the H2O2 generator.

WARNING When performing H2O2 decontamination, make sure that the outer and inner doors are securely closed. During H2O2 decontamination, plug the access hole with the silicon caps that are provided. Failure to do so may be harmful to health due to leakage of H2O2 gas.

CAUTION H2O2 decontamination can be performed only for the chamber and inner attachments with standard specifications, and not for any other objects.

CAUTION Perform H2O2 decontamination with the inner attachments arranged as specified by our company. Arranging them in a different way may result in insufficient decontamination.

CAUTION Wear rubber gloves when handling the H2O2 reagent. Direct contact with the H2O2 reagent may result in inflammation of the skin.

CAUTION After H2O2 decontamination has been completed, residual H2O2 solution will remain on the bottom of the chamber, the H2O2 generator, and the bottom of the duct. Wearing protective glasses and rubber gloves, wipe it off with a non-woven cloth. Failure to do so may result in a deficient culture.

42


Use the following procedure to perform H2O2 decontamination using the H2O2 generator (MCO-HP).

1. Take out all the trays, the tray supports, the side supports, the humidifying pan cover, the humidifying

pan, the fan cover, and the duct from the chamber. Dispose of the water in the humidifying pan, wipe the

inside walls of the chamber with a gauze containing water or alcohol for disinfection.

2. Attach the duct and side supports, and set the tray supports in the 2nd, 5th, 7th, 9th holes from the top

of the side supports. Then set the trays.

3. Pour one bottle of the H2O2 reagent (MCO-H2O2) into the H2O2 generator. Set the two pins on the

H2O2 generator in the 2 holes on the lower left side of the duct (Fig. 1).

4. Connect the H2O2 generator and connector on the bottom right of the far side of the chamber with the

enclosed cable. Be sure to keep the connector cap (Fig. 2).

5. Set the humidifying pan cover, the humidifying pan and the fan cover as shown in Fig. 3, and close the

inner door and the outer door.

Note: The trays included as accessory are designed to be appropriate for decontamination. If half tray

(MCO-25ST, optional) or trays for previous models are used, decontamination may not be sufficiently

effective.

Fig. 2 H2O2 generator

ConnectorDuct end

Fig. 1

Humidifying pan

Fan cover

Front

H2O2 Generator

Fig. 3

Humidifying pan cover

43

6. Press the H2O2 key (H2O2) for 5 seconds. The system check will start.

7. If the system is normal, the following display will appear. (If the system is not normal, refer to Table 2

on page 47.) Select “OK” and press the enter key (ENT) to start H2O2 decontamination. Decontamination

will be performed automatically to step 10. The buzzer will sound when decontamination has been

completed (It takes about 1 hour 40 minutes after warming-up).

Note: The outer door will be locked with the electric lock for safety until the decontamination has been

completed.

8. During the H2O2 mist generation, “H2O2 Decon” will blink at the top left corner of the screen.

Note: H2O2 mist generates from the H2O2 generator and then quickly changes to a gas.

1 H 2 O 2 D e c o n t a m i n a t i o n

3

4 R e a d y t o S t a r t ?

5

6

M E N U

O K

C a n c e l

1 H 2 O 2 D e c o n U V R e s o l v e S t e p : 4

2

3

4

5 T e m p : 4 5 . 0 ℃

6 D o o r : L o c k e d

7

７min ９０min

1 H 2 O 2 D e c o n t a m i n a t i o n

3

4 H 2 O 2 D e c o n S y s t e m C h e c k ・・・・・

5

6

44


9. After completion of H2O2 mist generation, “UV Resolve” will blink at the top of the screen, and H2O2 gas

resolution by UV will start.

10. After H2O2 gas resolution, H2O2 decontamination has been completed. The following display will

appear. Open the outer door and the inner door, then disconnect the cable of the H2O2 generator from the

connector in the chamber. Then take out the H2O2 generator.

Note: Always put on protection glasses and rubber gloves when performing decontamination.

11. The following display will appear. Press the menu key (MENU) to display the menu, select “OK”, and

press the enter key (ENT) to return to the “Top Display”.

1 H 2 O 2 D e c o n t a m i n a t i o n S t e p : 8

2

3 D e c o n F i n i s h e d .

4

5 T e m p : 4 5 . 0 ℃

6 P l e a s e R e m o v e H 2 O 2 U n i t D o o r : U n l o c k

7

1 H 2 O 2 D e c o n t a m i n a t i o n S t e p : 8

2

3 D e c o n F i n i s h e d .

4

5 T e m p : 4 5 . 0 ℃

6 P U S H M E N U O K D o o r : U n l o c k

7

1 H 2 O 2 D e c o n U V R e s o l v e S t e p : 7

2

3

4

5 T e m p : 4 5 . 0 ℃

6 D o o r : L o c k e d

7

０min ８９min

45

12. Dilute the remaining H2O2 reagent in the H2O2 generator with a large

volume of water and dispose of it. Rinse and wash the H2O2 generator with

distilled water. Then keep the H2O2 generator in a clean environment outside

of the chamber.

Note: After H2O2 decontamination, cover the connector on the chamber side

with the connector cap deeply. (Fig. 1)

13. After H2O2 decontamination, surplus H2O2 liquid will remain at the bottom

of the chamber and in the bottom part of the H2O2 generator duct. This

solution contains H2O2 at a low density (about 1 % or less), so put on

protective glasses and rubber gloves and wipe it up with a non-woven cloth.

14. Ventilate the chamber sufficiently and place all the attachments back into the chamber.

Note: Be sure to attach the connector cap.

CAUTION The electric lock will remained locked if power fails during H2O2 decontamination. After the power comes

back ON, the H2O2 gas resolution process will start execution and finish automatically. Execute the

decontamination again because the decontamination will be incomplete.

WARNING Never open the outer door by unlocking it with the unlock key during H2O2 decontamination or H2O2 gas

resolution with the UV lamp. H2O2 gas leakage is potentially harmful to health.

Precautions when handling H2O2 reagent

Observe the following precautions when handling the H2O2 reagent (MCO-H2O2).

Handling precautions

Wear protective equipment, such as protective glasses and rubber gloves.

Do not use fire in the area where the reagent is being handled.

Do not leave any reagent in the container after it has been used or while it is being used.

Do not place inflammable or combustible materials near the area where the reagent is handled.

Precautions for storage

Always close the container cover securely to prevent impurities from becoming mixed in the reagent.

Check the container to make sure that there is no damage, corrosion, or cracking.

Store the container with the inlet facing upwards, and make sure that the container will not tip or be

knocked over.

If possible, store the reagent in a location where water is available and where the floor can be easily

washed.

Precautions for disposal

Dispose according to the rule in your country.

Fig. 1

46

ALARMS, SAFETY, AND SELF-DIAGNOSIS The incubator supports the following alarms, safety functions, and self-diagnostic functions. Table 1: Alarms, safety, and self-diagnosis for culture operations

Alarm or safety function

Conditions Display Buzzer Safety operation

High limit temperature alarm

The chamber temperature exceeds the high limit alarm temperature set value.

“Err13: Main Heater Abnormal or Err16: CO2S Box Heater Abnormal” is displayed in the message display field.Overheat indicator lights.

Continuous tone Heater OFF Remote alarm operates

Automatic set temperature alarm

The chamber temperature is out of the automatic set temperature alarm setting range (±1.0 oC to ±5.0 oC).

“Warning :High Temp” or “Warning :Low Temp” is displayed in the message display field.

Intermittent tone after set alarm delay time (0 min to 15 min) has elapsed

Remote alarm operates after delay of 0 min to 15 min.

Automatic set CO2 density alarm

The chamber CO2 density is out of the automatic set CO2 density alarm setting range (±0.5 % to ±5.0 %).

“Warning :High CO2 Density” or “Warning :Low CO2 Density” is displayed in the message display field.


Remote alarm operates after delay of 0 min to 15 min.

Auto-return There are no key operations for approx. 90 s in a setting mode.

(Return to the “Top Display”.) ----- The setting mode is canceled.

Key lock The key lock is ON. “Key Lock” is displayed on the standby operation setting screen.

----- Setting changes are prohibited.

Door alarm The outer door is open. “Door：Open” is displayed in the message display field.


The CO2 valve is closed. The heater turns OFF after 1 min.

CO2 gas cylinder empty

The CO2 density does not increase when the CO2 valve is opened.

“Err01: CO2 Gas Empty” is displayed in the message display field.

Intermittent tone Remote alarm operates.

CO2 line changeover

The CO2 gas supply is switched automatically to the reserve cylinder (only when the MCO-21GC is installed).

“Err01: CO2 Gas Empty” is displayed in the message display field. The text blinks for the empty CO2 gas supply line.

〃

The CO2 gas supply is switched. Remote alarm operates.

Chamber temperature sensor error

The chamber temperature sensor is disconnected.

“Err05: Temp Sensor Open” is displayed in the message display field.

〃

The heater turns OFF. Remote alarm operates.

The chamber temperature sensor is short-circuited.

“Err06: Temp Sensor Short” is displayed in the message display field.

〃

The heater turns OFF. Remote alarm operates.

Sensor box temperature sensor error

The sensor box temperature sensor is disconnected.

“Err07: CO2S Box Temp Sensor Open” is displayed in the message display field.

〃 CO2 valve is closed. Remote alarm operates.

The sensor box temperature sensor is short-circuited.

“Err08: CO2S Box Temp Sensor Short” is displayed in the message display field.

〃 CO2 valve is closed. Remote alarm operates.

Ambient temperature sensor error

The ambient temperature sensor is disconnected.

“Err09: At Sensor Open” is displayed in the message display field.

〃 Remote alarm operates.

The ambient temperature sensor is short-circuited.

“Err10: At Sensor Short” is displayed in the message display field.


CO2 sensor error

The Vref or Vgas output voltage for the CO2 sensor is abnormal.

“Err11: CO2 Sensor Vref Abnormal” or “Err12: CO2 Sensor Gas Abnormal” is displayed in the message display field.

〃 CO2 valve is closed.Remote alarm operates.

Main heater error

Main heater burnout occurs or the main heater SSR is short-circuited when the high limit temperature alarm operates.

“Err13: Main Heater Abnormal” is displayed in the message display field.


Bottom heater error

Bottom heater burnout occurs or the bottom heater SSR is short-circuited.

“Err14: Humidity Heater Abnormal” is displayed in the message display field.


Door heater error

Door heater burnout occurs or the door heater SSR is short-circuited.

“Err15: Door Heater Abnormal” is displayed in the message display field.


Sensor box heater error

Sensor box heater burnout occurs or the sensor box heater SSR is short-circuited.

“Err16: CO2S Box Heater Abnormal” is displayed in the message display field.


Heater SSR burnout

Main, bottom, door, or sensor box heater SSR burnout occurs.

“Err17: Heater SSR Open” is displayed in the message display field.


Low humidifying water

The water in the humidifying pan drops to approx. 600 ml.

“RH PAN” blinks in reverse video in the status display field.

----- -----

47

Alarm or safety function

Conditions Display Buzzer Safety operation

UV lamp failure The UV lamp burns out (only for the MCO-19AIC(UVH)/19AIC(UV) or when the optional MCO-19UVS is installed).

“Err18: UV Lamp Abnormal” is displayed in the message display field.

Intermittent tone Remote alarm operates.

New UV lamp recommended

The accumulated ON time reaches approx. 1,000 h (only for the MCO-19AIC(UVH)/19AIC(UV) or when the optional MCO-19UVS is installed).

“UV” is displayed in the status display field. (When the UV lamp is ON) “UV” blinks in the status display field. (When the UV lamp is OFF) “Warning :UV Life” is displayed in the message display field.

----- -----

The buzzer can be stopped by pressing the buzzer key (BUZZER), but the remote alarms will not stop. In addition, the buzzer that sounds because of the high limit temperature alarm cannot be stopped by pressing the buzzer key (BUZZER). Err01 recovery is achieved automatically by correctly connecting the new CO2 gas cylinder and pressing the buzzer key (BUZZER) to stop the buzzer. In addition, when the MCO-21GC is installed and the gas supply is switched to the reserve gas cylinder, press the buzzer key (BUZZER) and replace the gas cylinder. If an error from Err05 to Err18 occurs (except for the high limit temperature alarm in Err13 to Err16), then contact our sales representative or agent.

Table 2: Alarms and Safety functions for H2O2 decontamination Alarm and safety

function Condition Display Buzzer Safety operation

System check error at start of H2O2 decontamination

The H2O2 generator is not connected.

“H2O2 Decon System Error” is displayed in the screen center. “Err31:H2O2 Unit No Connect” is displayed in the message display field.

----- Decontamination mode is cancelled.

There is no H2O2 solution or the H2O2 level sensor has failed (or is disconnected).

“H2O2 Decon System Error” is displayed in the screen center. “Err32: H2O2 Level is Low” is displayed in the message display field.


The door is not closed.

“H2O2 Decon System Error” is displayed in the screen center. “Err33: Door is Open” is displayed in the message display field.


Failure during warming-up

Power was interrupted. After power is restored, return to the “Top Display”.

----- -----

The outer door is open.

“Err38:Door Lock Failed” is displayed in the message display field. After completion, “Decon Failed” is displayed in the screen center.

Continuous tone (when the outer door is open.)

Decontamination mode is cancelled. Remote alarm operates. (when the outer door is open.)

Failure during H2O2 decontamination

The volume of H2O2 generated is abnormal.

“Err34:H2O2 Volume” is displayed in the message display field.

Intermittent tone with 15min delay

Moves to UV resolve. Remote alarm operates with 15 min. delay.

Power was interrupted during H2O2 decontamination.

After power is restored, “Err35: Power Failed” is displayed in the message display field. After completion, “Decon Failed” is displayed in the screen center.

Intermittent tone

Electrically locked during power interruption. After power is restored, moves to UV resolve.Remote alarm operates.




H2O2 mist generation is cancelled. Resolve time is extended. Remote alarm operates. (when the outer door is open.)

Failure during H2O2

gas resolve by UV.

The UV lamp failed during H2O2 gas resolve by UV.

“Err36: UV Lamp Failure” is displayed in the message display field.

Intermittent tone

Resolve time is extended. Remote alarm operates.

Power was interrupted during H2O2 gas resolve by UV.

After power is restored, “Err37: Power Failed” is displayed in the message display field.

-----

Electrically locked during power interruption. After power is restored, UV resolve is repeated.




Resolve time is extended. Remote alarm operates. (when the outer door is open.)

48

ALARMS, SAFETY, AND SELF-DIAGNOSIS

Table 3: Alarm and Safety functions for automatic standard gas calibration Alarm and safety

function Condition Display Buzzer Safety operation

System check error at start/operating of automatic CO2 density calibration

The gas pressure of CO2 gas line for the standard gas calibration is less than the specified pressure.

“Std Gas Calib Error” is displayed in the screen center. “Err41: CO2 Std Gas Empty” is displayed in the message display field.

Intermittent tone

The automatic CO2 density calibration is cancelled. Remote alarm operates.

Note:

For models with electric locking, an unlock key is provided in order to unlock the outer door during a

power interruption or in case the electric lock fails. Always store this key in a safe place. It is

recommended that you make a note of the key symbol and number in case the key is lost.

WARNING Do not use the unlock key to unlock the outer door during H2O2 decontamination or during H2O2

gas resolve by UV. Doing so may cause harm to health from H2O2 gas leakage.

49

CALIBRATION

Temperature/CO2 calibration


enter key (ENT).

2. Select “Temp/CO2 Calibration” from the “Select Tools (1/2)” display, press the menu key (MENU) to

display the menu, select “OK”, and press the enter key (ENT).

3. The “Temp/CO2 Calibration” display will appear.

Example

Enter the value shown below if the displayed chamber temperature is 37.0 oC but the actual temperature

is 36.5 oC.

●Temperature span: Enter 365.

S e l e c t T o o l s ( 1 / 2 )




U V S e t t i n g


D a t e T i m e

M E N U

O K

S v c

C a n c e l

1 T e m p 3 7 . 0 o C C O 2 5 . 0 %

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０ M E N U

S e t

L o g

T o o l s

0

1

0

2

0

3

0

4

0

5

0

6

0

7

0

8

0

9

1

0

1

1

1

2

1

3

1

4

1

5

1

6

1

7

1

8

1

9

2

0

2

1

2

2

2

3

2

4

2

5

2

6

2

7

2

8

2

9

3

0

3

1

3

2

3

3

3

4

3

5

3

6

3

7

3

8

3

9

4

0

1 T e m p / C O 2 C a l i b r a t i o n

3 P V V o l t

T e m p S p a n 3 6 . 5 o C 3 7 . 0 o C 0 . 0 ° C

4 C O 2 Z e r o 0 . 1 % 0 . 0 % 4 . 0 2 V 5 0 0

5 C O 2 S p a n 0 . 0 % 5 . 0 % 4 . 0 1 V 5 0 0

C O 2 R e f 4 . 0 2 V

6 C O 2 G a s 4 . 0 1 V

50

CALIBRATION

Example

Enter the value shown below if the displayed chamber CO2 density is 5.0 % but the actual CO2 density is

4.5 %.

●CO2 density span: Enter 045.

4. Press the menu key (MENU) to display the menu, select “OK”, and press the enter key (ENT).

5. After the “Select Tools (1/2)” display is displayed, press menu key (MENU) to display the menu, select

“Cancel”, and press the enter key (ENT) to return to the “Top Display”.

Note:

It is important to accurately measure the temperature inside the incubator when calibrating the

temperature. Particularly, the temperature gauge used must have an accuracy of JIS 0.5 Class or better.

The temperature must be measured at several points.

The temperature setting must not change by more than ±1.0 oC during calibration. If these values are

exceeded, an error buzzer will sound, the input data will be ignored, and the incubator will return to the

display mode. If it is necessary to change the temperature by more than 1.0 oC, perform calibration in

several stages over a period of time.

0

1

0

2

0

3

0

4

0

5

0

6

0

7

0

8

0

9

1

0

1

1

1

2

1

3

1

4

1

5

1

6

1

7

1

8

1

9

2

0

2

1

2

2

2

3

2

4

2

5

2

6

2

7

2

8

2

9

3

0

3

1

3

2

3

3

3

4

3

5

3

6

3

7

3

8

3

9

4

0

1 T e m p / C O 2 C a l i b r a t i o n

3 P V V o l t

T e m p S p a n 0 . 0 o C 3 7 . 0 o C 0 . 0 ° C

4 C O 2 Z e r o 0 . 0 % 4 . 0 2 V 5 0 0

5 C O 2 S p a n 4 . 5 % 5 . 0 % 4 . 0 1 V 5 0 0

C O 2 R e f 4 . 0 2 V

6 C O 2 G a s 4 . 0 1 V

51

TROUBLESHOOTING

If the incubator does not seem to be working properly, check the following items before calling for service.

Symptom Items to check and countermeasures

The incubator does not operate at all.

Is the incubator plugged in?

Is there a power outage, or has a circuit breaker interrupted the power?

The key is not working. Is the key lock function turned OFF?

An alarm has occurred. When starting operation:

Does the chamber temperature match the set value?

Does the CO2 gas density in the chamber match the set value?

(1) Is the secondary pressure for the gas regulator at the specified value of 0.03 MPa(G) (0.3 kgf/cm2(G), 4.3 psi(G))?

(2) Is the gas tube properly connected?

During operation:

Is the high limit alarm temperature set at least 1 C higher than the chamber set temperature?

Has the temperature setting been changed? Has the outer door been left open for a long time? Has a low-temperature object been placed in the chamber? If any of these is the case, the alarm will be automatically cleared if you wait.

Has the gas tube come loose, or is there a gas leak?

Has the CO2 gas density setting been changed?

Is the gas cylinder empty? Check the primary pressure of the gas cylinder once a week. (When the primary pressure is 3.8 MPa(G) (38 kgf/cm2(G), 5.5 kpsi(G)) or lower, it is a sign that there is little gas remaining. Replace the cylinder soon.)

Is the incubator operating beside the appliance that generates the electromagnetic wave?

The chamber temperature does not match the set value.

Is the ambient temperature less than 5 C different from the set value for the chamber temperature?

Is the outer door closed with the inner door left open?

The CO2 gas density does not match the set value.

Is the secondary pressure for the gas regulator at the specified value of 0.03 MPa(G) (0.3 kgf/cm2(G), 4.3 psi(G))?

Is the gas tube blocked?

The chamber temperature does not rise.

Is there enough water in the humidifying pan? (Be sure to use sterile distilled water.)

A large quantity of CO2 gas is being consumed.

Are the outer and inner doors being frequently opened and closed?

Check whether gas is leaking from connectors due to deterioration of the gas tube, or whether there may be any pinhole leaks. The gas tube is a replaceable part, and it is recommended that it be replaced once a year.

Is the packing seal for the inner door defective?

Is the access hole open?

Normal cultures are not possible, and the CO2 gas density is suspect.

Is the ambient air environment around the incubator normal? Is there a source of polluted gas in the vicinity?

52

TROUBLESHOOTING

Symptom Items to check and countermeasures The CO2 gas density is slow to recover.

A HEPA filter is used for the incubator CO2 gas piping. If gas density is slow to recover when the CO2 gas pressure is normal, it is possible that the HEPA filter may be clogged. Contact our sales representative or agent.

Is there little gas remaining in the CO2 gas cylinder?

Is the secondary pressure for the gas regulator at the specified value of 0.03 MPa(G) (0.3 kgf/cm2(G), 4.3 psi(G))?

Is the gas tube blocked?

CO2 gas is not being injected. The CO2 control method for the incubator is the ON-OFF method. CO2 gas is intermittently injected as the gas density in the chamber approaches the set value. Injections may be stopped for periods of approximately 15 seconds, but that is not an error.

The outer door does not open. MCO-19AIC(UVH), or when the optional MCO-HL is installed:

When the power switch is OFF, the electric lock is locked and the outer door does not open. Either turn ON the power switch or use the accessory unlock key to override the electric lock.

During decontamination the outer door is electrically locked and does not open.

H2O2 decontamination cannot be performed.

If the MCO-19AIC(UV)/19AIC is being used, is the optional MCO-HL installed?

If the MCO-19AIC is being used, are the optional MCO-HL and MCO-19UVS installed?

Is the UV lamp burned out? If the UV lamp is burned out, H2O2

decontamination will not be possible.

Is the H2O2 generator cable properly connected?

Has the entire bottle of the H2O2 reagent been used?

Has the high limit temperature alarm been set to 50 C or higher?

In H2O2 decontamination cycle,

”Err34:H2O2 Volume “ occurred.

Is the duct securely attached? Attach the duct properly to the 4 points hooks. (Fig. 1)

Is the fan attached properly? Remove the central spring at the center of the fan and confirm if the fan is pushed all the way to the motor shaft. Then, reinstall the central spring. (Fig. 2)

Is the H2O2 generator securely installed? Set the 2 pins of the H2O2 generator in the 2 holes at the left bottom of the duct. (Fig. 3)

Note: If the problem still has not been solved after trying the above checks and countermeasures, or for any problems not covered here, contact our sales representative or agent.

① Push the fan deeply.

② Install the central spring afterwards.

③ Lightly turn the fan manually to make sure that it does not touch the rear panel.


53

DISPOSAL OF UNIT

When disposing of the CO2 incubator, contact our sales representative or agent.

WARNING The CO2 incubator must be dismantled and disposed of by qualified personnel only. If the CO2

incubator is left where outsiders enter, it may result unexpected accident (for example, children to become

locked inside).

Before disposing the CO2 incubator with biohazardous danger, decontaminate the CO2 incubator to the

extent possible by the user.

54

DISPOSAL OF UNIT

Waste Electrical and Electronic Equipment (WEEE) Directive 2002/96/EC

(English)

This Panasonic product is designed and manufactured with high-quality materials and components that

can be recycled and reused.

This symbol means that the electrical and electronic equipment, at their end-of-life, should be disposed of

separately from household wastes.

Dispose of this product at your local community waste collection/recycling center.

In the European Union there are separate collection systems for used electrical and electronic products.

Please help us to conserve the environment we live in.

(German)

Ihr Panasonic Produkt wurde entworfen und hergestellt mit qualitativ hochwertigen Materialien und

Komponenten, die recycelt und wiederverwendet werden können.

Dieses Symbol bedeutet, daß elektrische und elektronische Geräte am Ende ihrer Nutzungsdauer von

Hausmüll getrennt entsorgt werden sollen.

Bitte entsorgen Sie dieses Gerät bei Ihrer örtlichen kommunalen Sammelstelle oder im Recycling Centre.

In der Europäischen Union gibt es unterschiedliche Sammelsysteme für Elektrik- und Elektronikgeräte.

Helfen Sie uns bitte, die Umwelt zu erhalten, in der wir leben!

Note: This symbol mark and recycle system apply only to EU countries. They do not applied to the countries in other areas of the world.

55

(French)

Votre produit Panasonic est conçu et fabriqué avec des matèriels et des composants de qualité supérieure

qui peuvent être recyclés et réutilisés.

Ce symbole signifie que les équipements électriques et électroniques en fin de vie doivent être éliminés

séparément des ordures ménagères.

Nous vous prions donc de confier cet équipement à votre centre local de collecte/recyclage.

Dans l’Union Européenne, il existe des systèmes sélectifs de collecte pour les produits électriques et

électroniques usagés.

Aidez-nous à conserver l’environnement dans lequel nous vivons !

Les machines ou appareils électriques et électroniques contiennent fréquemment des matières qui, si elles

sont traitées ou éliminées de manière inappropriée, peuvent s’avérer potentiellement dangereuses pour la

santé humaine et pour l’environnement.

Cependant, ces matières sont nécessaires au bon fonctionnement de votre appareil ou de votre machine.

Pour cette raison, il vous est demandé de ne pas vous débarrasser de votre appareil ou machine usagé

avec vos ordures ménagères.

(Spanish)

Los productos Panasonic están diseñados y fabricados con materiales y componentes de alta calidad,

que pueden ser reciclados y reutilizados.

Este símbolo significa que el equipo eléctrico y electrónico, al final de su ciclo de vida, no se debe

desechar con el resto de residuos domésticos.

Por favor, deposite su viejo “televisor” en el punto de recogida de residuos o contacte con su

administración local.

En la Unión Europea existen sistemas de recogida específicos para residuos de aparatos eléctricos y

electrónicos.

Por favor, ayúdenos a conservar el medio ambiente!

56

DISPOSAL OF UNIT

(Portuguese)

O seu produto Panasonic foi concebido e produzido com materiais e componentes de alta qualidade que

podem ser reciclados e reutilizados.

Este símbolo significa que o equipamento eléctrico e electrónico no final da sua vida útil deverá ser

descartado separadamente do seu lixo doméstico.

Por favor, entregue este equipamento no seu ponto local de recolha/reciclagem.

Na União Europeia existem sistemas de recolha separados para produtos eléctricos e electrónicos

usados.

Por favor, ajude-nos a conservar o ambiente em que vivemos!

(Italian) Il vostro prodotto Panasonic è stato costruito da materiali e componenti di alta qualità, che sono

riutilizzabili o riciclabili.

Prodotti elettrici ed elettronici portando questo simbolo alla fine dell’uso devono essere smaltiti

separatamente dai rifiuti casalinghi.

Vi preghiamo di smaltire questo apparecchio al deposito comunale.

Nell’Unione Europea esistono sistemi di raccolta differenziata per prodotti elettrici ed elettronici.

Aiutateci a conservare l’ambiente in cui viviamo!

57

(Dutch)

Panasonic producten zijn ontwikkeld en gefabriceerd uit eerste kwaliteit materialen, de onderdelen kunnen

worden gerecycled en weer worden gebruikt.

Het symbool betekent dat de elektrische en elektronische onderdelen wanneer deze vernietigd gaan

worden , dit separaat gebeurt van het normale huisafval.

Zorg ervoor dat het verwijderen van de apparatuur bij de lokaal erkende instanties gaat gebeuren.

In de Europese Unie wordt de gebruikte elektrische en elektronische apparatuur bij de daarvoor wettelijke

instanties aangeboden.

Alstublieft help allen mee om het milieu te beschermen.

(Swedish)

Din Panasonic produkt är designad och tillverkad av material och komponenter med hög kvalitet som kan

återvinnas och återanvändas.

Denna symbol betyder att elektriska och elektroniska produkter, efter slutanvändande, skall sorteras och

lämnas separat från Ditt hushållsavfall.

Vänligen, lämna denna produkt hos Din lokala mottagningstation för avfall/återvinningsstation.

Inom den Europeiska Unionen finns det separata återvinningssystem för begagnade elektriska och

elektroniska produkter.

Vänligen, hjälp oss att bevara miljön vi lever i!

58

AUTOMATIC CO2 CYLINDER CHANGEOVER

A gas auto changer (MCO-21GC) is available as an optional accessory. This kit switches the gas supply

lines when one of the CO2 gas cylinders becomes empty.

Note: The installation of the MCO-21GC must be performed only by qualified service personnel.

Install the MCO-21GC and then perform the following procedure.

1. Connect 2 gas tubes to the connecting ports A and B for CO2 gas pipe. The connecting ports A and B

for CO2 gas pipes are located on the left back of the incubator. (Refer to page 8).

Note: Only the port A can be used when the MCO-21GC is not installed. There will be 2 ports, the ports A

and B, after the MCO-21GC is installed.

2. Connect a CO2 gas cylinder equipped with a gas regulator to each gas tube. Refer to page 14 for

information on connecting the gas cylinders. (“Gas cylinder A” is the CO2 gas cylinder connected to the

connecting port A for CO2 gas pipe, and “gas cylinder B” is the CO2 gas cylinder connected to the

connecting port B for CO2 gas pipe.)

3. Open the valves of both gas cylinders.

4. The current CO2 gas supply line is displayed on the “Top Display”.

(“A” and “B” will be displayed only when the MCO-21GC is installed. Nothing will be displayed if

MCO-21GC is not installed.)

5. The MCO-21GC detects that there is no more CO2 gas in a cylinder when the CO2 density in the

chamber does not increase for a while even after opening the CO2 gas valve in the incubator. It switches

the gas supply line when it detects that there is no more gas. At that time, the indications of switching the

gas cylinder are as follows:

Message display field Display of gas supply

When CO2 gas supply line switches

automatically (Only when MCO-21GC

is installed.)

Err01: CO2 Gas Empty is

displayed.

The character for gas supply line

that is empty blinks.

The gas supply line that is

currently being used is displayed

in reverse video.

●To silence the buzzer, press the buzzer key (BUZZER). The alarm condition is cleared and normal

operation continues.

Current CO2 gas supply line

1 T e m p 3 7 . 0 o C C O 2 5 . 0 % A B

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０

59

6. When the gas cylinder A is switched to the gas cylinder B, remove gas cylinder A and replace it with

new one.

Note: Be careful when handling the empty CO2 gas cylinder. Some gas may still be left in the cylinder.

●When the gas cylinder B becomes empty, the line is switched to the gas cylinder A again.

The MCO-21GC detects that there is no more CO2 gas in a cylinder when the CO2 density in the chamber

does not increase for a while even after opening the CO2 gas valve in the incubator. The supply line may

also be switched for other reasons even though there is gas left in the cylinder: blocking or restricting of

gas tube, reduction of CO2 gas pressure, or improper opening of CO2 gas cylinder. Always check the gas

quantity in the cylinder before disconnecting it.

● Use the following procedure to manual switch the line between CO2 cylinders A and B (only when

MCO-21GC is installed). This procedure shows switching the line from the gas cylinder B to the gas

cylinder A as an example.

1. From the “Top Display”, press the “Left Cursor Key” for 5 seconds.

2. The CO2 gas supply line will be switched from the gas cylinder B to the gas cylinder A.

(When the line needs to be switched from the gas cylinder A to the gas cylinder B, press the “Right

Cursor Key” for 5 seconds.)

1 T e m p 3 7 . 0 o C C O 2 5 . 0 % A B

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０

1 T e m p 3 7 . 0 o C C O 2 5 . 0 % A B

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０

60

AUTOMATIC CO2 DENSITY CALIBRATION

To calibrate the CO2 density, a STD gas auto calibration kit (MCO-SG) is available. By connecting a CO2

standard gas cylinder that has the density that is the same as the set CO2 density (usually 5 % CO2), the

CO2 sensor can be calibrated to maintain the accuracy of the CO2 density display as necessary.

● Setting the connected standard CO2 gas density


enter key (ENT).

2. Press the “Down Cursor Key” from the “Select Tools (1/2)” display to display the “Select Tools (2/2)”

display, and then select “Standard Gas Setting”, press the menu key (MENU) to display the menu, select

“OK”, and press the enter key (ENT).

1 T e m p 3 7 . 0 o C C O 2 5 . 0 %

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０ M E N U

S e t

L o g

T o o l s

S e l e c t T o o l s ( 2 / 2 )

S t a n d a r d G a s S e t t i n g

S t a n d a r d G a s C a l i b r a t i o n

M E N U

O K

S v c

C a n c e l

S e l e c t T o o l s ( 1 / 2 )




U V S e t t i n g


D a t e T i m e

M E N U

O K

S v c

C a n c e l

61

3. Input the CO2 density of standard gas to be used, press the menu key (MENU) to display the menu,


● Calibration method for CO2 gas density

1. Select “Standard Gas Calibration” from the “Select Tools (2/2)” display, press the menu key (MENU) to

display the menu, select “OK”, and press the enter key (ENT).

2. The system check will start.

S e l e c t T o o l s ( 2 / 2 )

S t a n d a r d G a s S e t t i n g

S t a n d a r d G a s C a l i b r a t i o n

M E N U

O K

S v c

C a n c e l

1 S t a n d a r d G a s S e t t i n g

3

C O 2 S T D G a s D e n s i t y 5 . 0 % %

4

5

6

M E N U

O K

C a n c e l

1 S t a n d a r d G a s C a l i b r a t i o n S t e p ： 1

3

S t d G a s C a l i b S y s t e m C h e c k ・・・・・

4

5 C O 2 ： 0 . 0 %

S T G ： 5 . 0 %

6

62

AUTOMATIC CO2 DENSITY CALIBRATION

3. If the system is normal, the following display will appear. Select “OK” and press the enter key (ENT) to

start calibrating the CO2 density. After calibration, return to normal cultivating operation.

1 S t a n d a r d G a s C a l i b r a t i o n

3

R e a d y t o S t a r t ?

4

5 C O 2 ： 0 . 0 %

S T G ： 5 . 0 %

6

M E N U

O K

C a n c e l

1 S t a n d a r d G a s C a l i b r a t i o n S t e p ： 3

3

S t d G a s C a l i b r a t i n g ・・・・・

4

5 C O 2 ： 4 . 8 %

S T G ： 5 . 0 %

6

1 T e m p 3 7 . 0 o C C O 2 5 . 0 %

2

3

4

5 S t a t u s

6 O K

7

３７.０５.０

63

STACKING INCUBATORS

Use the following procedure to stack incubators. This work is potentially dangerous, so contact our sales

representative or agent.

CAUTION Select a floor that is strong enough to support the stacked incubators.

Never stack 3 or more incubators. Doing so is dangerous.

1. Take out 2 stacking plates A, 2 stacking plates B, 4 protective stickers, and 4 screws from the

accessory bag.

Note:

When stacking 2 incubators, use 2 stacking plates (A and B) for each one. Only one pair of stacking

plates is provided per an incubator, so take out the stacking plates (A and B) from both incubators.

Similarly only 2 protective stickers are provided per an incubator

2. Unplug both of the incubators that are to be stacked.

3. Remove the caps at the 2 places in the front of the top surface of the incubator that is to be on the

bottom, attach the protective stickers to the 4 corners on the top surface of the incubator that is to be on

the bottom. (Fig. A)

4. Secure stacking plate A with the screws that are provided.

5. Remove the front panel (5 screws) from the incubator that is to be on the top.

6. Place the upper incubator on top of the lower one, being careful not to pinch your fingers.

7. Make the lower incubator level by adjusting the leveling feet, and then level the upper incubator in the

same way.

8. Secure the stacking plate A fixed to the lower incubator to the upper incubator with the accessory

screws.

9. Remove from the back of the lower incubator the 2 hooks (4 screws) and remove from the upper

incubator the 2 screws on the lower rear panel.

10. Use the 6 screws that were removed in step 9 to secure stacking plate B to the rear panels of the

upper and lower incubators.

11. Replace the front panel (5 screws) to the upper incubator.

12. Use the hooks on the back of the upper incubator to prevent the incubators from falling over.

64

STACKING INCUBATORS

Fig. A

Stacking plate B

Stacking plate A

Protective sticker

Front panel

Hook

Front

65

SPECIFICATIONS

Product name CO2 incubator

MCO-19AIC

CO2 incubator

MCO-19AIC(UV)

CO2 incubator

MCO-19AIC(UVH)

External dimensions W620 mm x D710 mm x H900 mm (W24.4 inch x D28.0 inch x H35.4 inch)

Internal dimensions W490 mm x D523 mm x H665 mm (W19.3 inch x D20.6 inch x H26.2 inch)

Interior volume 170 L (6.00 cu.ft.)

Exterior Painted steel

Interior Stainless steel containing copper

Outer door Painted steel

Inner door Tempered glass

Trays

4 trays made of stainless steel containing copper

W450 mm x D450 mm x H12 mm (W17.7 inch x D17.7 inch x H0.47 inch)

Maximum load: 7 kg/tray

Access port Inner diameter: 30 mm (1.18 inch), On the back side

Insulation Rigid polyurethane foamed-in place

Heating system DHA system (heater jacket + air jacket system)

Heater 314 W

Humidifying system Natural evaporation with humidifying pan

Temperature controller PID control system

Temperature display Digital display

CO2 controller PID control system

CO2 density display Digital display

Air circulation Fan assisted

Air filter 0.3 m, Efficiency: 99.97 % or higher

UV lamp ----- 4W x 1 (ozone-free emission)

Water level sensor Optical

Alarms Automatic set temperature alarm, Automatic set CO2 density alarm, High limit temperature alarm, CO2 gas, various sensor/heater alarms

Remote alarm contacts Allowable contact capacity: DC 30 V, 2 A

CO2 inlet connection 4 mm to 6 mm (0.157 inch to 0.236 inch) diameter tube can be connected

CO2 inlet pressure 0.03 MPa(G) (0.3 kgf/cm2(G), 4.3 psi(G))

Weight 93 kg

Accessories 4 trays, 4 sets of tray supports, 1 gas tube, 1 humidifying pan,

1 pair of stacking plates A and B, 2 protective stickers, 2 tube bands

66

SPECIFICATIONS

Product name CO2 incubator

MCO-19AIC

CO2 incubator

MCO-19AIC(UV)

CO2 incubator

MCO-19AIC(UVH)

Optional accessories

H2O2 generator (MCO-HP) H2O2 decon set (MCO-HL)

H2O2 generator and H2O2 decon set are standard equipment

UV system set (MCO-19UVS)

UV system set is a standard equipment.

H2O2 reagent (MCO-H2O2)

STD gas auto calibration kit (MCO-SG)

Gas regulator (MCO-100L)

Tray (MCO-47ST, includes 2 tray supports)

Half tray (MCO-25ST), Gas auto changer (MCO-21GC)

Roller base (MCO-18RB), UV replacement kit (MCO-20UV)

Stacking plate (MCO-18SB, when stacking on the MCO-175)

Stacking plate (MCO-21SB, when stacking on the MCO-20AIC)

Interface board (MTR-L03), Interface board (MTR-480)

Data acquisition system (MTR-5000)

interface board (MCO-420MA)

Note: Refer to the updated catalog when ordering an optional component.

Designs and specifications are subject to change without notice.

PERFORMANCE

Product name

CO2 Incubator MCO-19AIC

MCO-19AIC(UV)

MCO-19AIC(UVH)

Model No.

MCO-19AIC-PT

MCO-19AICUV-PT

MCO-19AICUVH-PT

MCO-19AIC-PA

MCO-19AICUV-PA

MCO-19AICUVH-PA

MCO-19AIC-PK

MCO-19AICUV-PK

MCO-19AICUVH-PK

Temperature control range Ambient temperature+5 oC to 50 oC (ambient temperature: 5 oC to 35 oC)

Temperature distribution ±0.25 oC (ambient temperature: 25 oC, setting: 37 oC, CO2: 5 %, no load)

Temperature variation ±0.1 oC (ambient temperature: 25 oC, setting: 37 oC, CO2: 5 %, no load)

CO2 control range 0 % to 20 %

CO2 variation ±0.15 % (ambient temperature: 25 oC, setting: 37 oC, CO2: 5 %, no load)

Chamber humidity 95 %R.H.±5 %R.H.

Applicable environment

condition

Temperature: 5 oC to 35 oC, Humidity: 80 %R.H. max.

(The designed performance may not be obtained

If the ambient temperature is equal or less than 15 oC)

Noise level 30 dB (A scale)

Power consumption Max. 350 W Max. 310 W

Heat emission Max. 1,120 kJ/h

Rated voltage, frequency 110 V to 120 V, 60 Hz 220 V, 60 Hz

Amperage Max. 2.8 A Max. 1.4 A

Note: The unit with CE mark complies with EC directives.

Based on our measuring method.

67

CAUTION

Please fill in this form before servicing. Hand over this form to the service engineer to keep for his and your safety.

Safety check sheet

1. Unit contents :

Risk of infection:

Risk of toxicity:

Risk from radioactive sources:

(List all potentially hazardous materials that have been stored in this unit.)

Notes :

2. Contamination of the unit

Unit interior

No contamination

Decontaminated

Contaminated

Others:

3. Instructions for safe repair/maintenance of the unit

a) The unit is safe to work on

b) There is some danger (see below)

Procedure to be adhered to in order to reduce safety risk indicated in b) below.

Date :

Signature :

Address, Division :

Telephone :

Product name :

CO2 incubator

Model No.

MCO-

Serial number : Date of Installation :

Please decontaminate the unit yourself before calling the service engineer.

□Yes □No□Yes □No

□Yes □No

□Yes □No

□Yes □No

□Yes □No

□Yes □No

□Yes □No

Printed in Japan 7FB6P15152900 S0412-1042

1-1-1, Sakata Oizumi-Machi Ora-Gun, Gunma 370-0596, Japan

© Panasonic Healthcare Co., Ltd. 2012

instruction manual mco-19aic-pe

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