integra&ng omero into a correla&ve microscopy workflow
TRANSCRIPT
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Integra(ng OMERO into a correla(ve microscopy workflow: successes/struggles
Damir Sudar and many others OME workshop -‐ June 5, 2014
Oregon Health & Science University, Lawrence Berkeley Na(onal
Laboratory, Quan(ta(ve Imaging Systems, Inc.
OHSU Center for Spatial Systems Biomedicine
Lawrence BERKELEY national LaBoratory: Life sciences Division
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OMERO use: Research context
• OHSU: iLEM for Mul(scale Structural Epigenomics
• LBNL: Integrated Bioimaging Ini(a(ve – Drosophila Gene Localiza(on Atlas – Next Genera(on BioImaging project – Integrated Imaging of Microbial Community Response to External Threats
• OHSU: Various drug screens, RNAi aberra(on screens, and microenvironment screens
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iLEM mo(va(on: Understanding regulatory signal transduc(on
Elucida(ng the roles of the nano-‐ and microenvironments Extracellular receptors
Intracellular signal transduc(on assemblies
Cellular ac(on centers
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Prototype problem RTK signaling in breast cancer
Normal cells have established regulatory systems to control behavior (grow, die, move,
secrete a protein, etc.)
ERBB2
Cancers can arise because of gene(c damage that deregulates RTK signaling
Our goal in cancer treatment is to develop therapies to correct the defect
lapa(nib
RTK – Receptor tyrosine kinase
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Ac(va(on of bypass mechanisms can a`enuate response
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ERBB2
lapa(nib Release of inhibitory cross-‐talk
ac(vates MAPK signaling
MEK inhibitor
There are many bypass pathways
Combinatorial Rx becomes increasingly toxic unless targe(ng is very precise
How does all of this informa(on get integrated?
We don’t know where or how it gets translated into
ac(on (prolifera(on, death, etc)
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Signaling from the microenvironment also can bypass the inhibitor
ERBB2
lapa(nib
MEK inhibitor
Signaling from the microenvironment
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We need to know how the parts are assembled in order to fully understand func(on (and response to therapy)
Moving from the parts list to the assembly manual
You can’t understand something from looking at the parts
You need to know how they work together
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These studies mo(vate the development of strategies to visualize pathways in situ
Basal breast cancer CK5/6 green, CK17 orange, CD44 red, DAPI blue
Defining the microenvironment
iLEM will define sub-‐cellular and micro-‐environmental context
Pathway nanostructure
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FEI CorrSight
FEI Helios
Olympus Scan^R
OMX
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Correla(ve Microscopy • Find specific cells in a large field of view • Find specific areas in a cell
and zoom in to study these at the ultrastructural level
• How? – Use two different microscopes and correlate the informa(on (two machines or combine in one)
– Use a machine that has a very large magnifica(on/resolu(on range and mul(modal imaging
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TEM
FM
Truly Integrated Light and Electron Microscopy (iLEM)
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Op,mal Naviga,on Tool for correla,ve microscopy
• Faster correla(on between FM and EM • Less poten(al contamina(on (cryo) • No compromise on EM performance, decline in FM resolu(on
Agronskaia AV et al., J Struct Biol. 2008
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Sample prep is cri(cal and difficult to op(mize
THP-‐1 cells fixed with VIS2FIXFS and IF+IG labeled for LAMP-‐2 (a lysosomal protein)
Karreman and Donselaar et al., Traffic 2011
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Managing the image data, metadata, and experimental parameters
• Image data and image metadata: OMERO • Experimental metadata: ISA-‐Tab & various Minimum Informa(on formats (MIARE, MIACA, )
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Image acquisi(on workflow
OMERO server w/ 10TB
Scan^R and other light microscopes
Tecnai w/ iCorr
Helios FIB/SEM
Titan Krios TEM
Utrecht -‐ Netherlands
Eindhoven -‐ Netherlands
TeraSta(on
TeraSta(on
TeraSta(on
LBNL
UCB
Other sites
Local disk
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Our experience • Likes:
– Easy setup (aoer dealing with OHSU firewall issues) – Excellent retrieval performance: inside/outside OHSU – Fully user-‐ini(ated image uploads and image retrieval – Good support for our image/experiment types
• Wishes: – Support for rich experimental metadata (e.g. ISA-‐Tab) – Bulk image download – Import using OMERO.web – Deeper folder structure
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2D iLEM workflow w/ iCorr
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3D iLEM workflow w/ FIB/SEM
ü Modular fluorescent probes ü Tracking during growth ü Super resolu(on capable ü EM sample prep compa(ble
ü Mul(cellular growth (Organovo) ü Prin(ng wide spectrum of (ssues ü Disease oriented research ü Live cell imaging
ü Cryo-‐immobiliza(on (HPF & plunge) ü Preserva(on of probes in EM prep ü Mul(ple EMs (Krios, Helios, iCorr) ü MAPS correla(on of SEM data
ü Development of Integra(ve Sooware ü 3D rendering & reconstruc(on ü Precision analysis of 3D data ü Mapping molecular informa(on into 3D
Integra(ng molecular informa(on and structural detail with nanometer precision
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Integrating molecular information and structural detail with nanometer precision
2 µm
Cell = Blue / Vesicles = Yellow Degraded ECM = Orange
Helios Nanolab 650 FIB-‐SEM
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Our experience • Likes:
– Large images are handled well – Scrip(ng – Flexible data access op(ons
• Wishes: – Registra(on informa(on between imaging modali(es i.e. a Real-‐World coordinate system
– Tiled/chunked storage of nD data in mul(ple scales – Automated segmenta(on methods
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Image analysis/visualiza(on workflow
OMERO server w/ HDF tables
AMIRA and other FEI sooware
Local worksta(on
Utrecht -‐ Netherlands
Local disk
LBNL
QI
LUMC
QI image analysis sooware
OMERO.Insight OMERO.web
Custom sooware (Java, C++, ImageJ/Fiji)
HT cluster
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Image analysis and visualiza(on " Find individual cells!
" Find sub cellular regions: Nucleus, Cytoplasm, Membrane!
" Measure biomarker expressions in each region!
" Relate channels: e.g. pAkt versus total Akt!
" Show statistics & relate statistics to morphology of cells and tissue!
" Compare over time and perturbation conditions!
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Acquired MRI
PALM
EM RegistrationTag
Computed Layers
(X,Y)
(X,Y)
(X,Y)
(X,Y)
(X,Y)
Histology Resolution
FL Layers
Mul(Scale Viewer
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Qi Fluorescence Biomarker Quantitation
Image & Metadata Database
Data Viewers
EM Image Analysis
Biological Experiments!
Data Visualization!Hypotheses!
Omics Information!
Image Analysis!
Image Acquisition!
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Our experience • Likes:
– Good Python support – Access to original data via “Archived” or “File paths” – HDF support for feature data
• Wishes: – Improved Java and C++ API support and documenta(on – Well defined infrastructure to ini(ate server-‐side processing on HPC cluster
– Easy use/display/searching of the HDF data – Abstracted shape descrip(ons of objects in close rela(onship with pixel/voxel descrip(ons
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Acknowledging Manfred Auer, Walter Georgescu, Aris Polyzos, David Skinner, Joaquin Correa, Erwin Frise
Joe Gray, Danielle Jorgens, Anke Mulder, Guillaume Thibault, Michel Nederlof (QI), Jan Post (UU), Elly van Donselaar (UU)
And the OME team!!!
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