interpretation of anaerobic condition
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Interpretation of anaerobic condition,growth of microbes in a naerbic chamberTRANSCRIPT
INTERPRETATION OF ANAEROBIC CONDITION
BY SREEREMYA.S
Pyrogallic acid-sodium hydroxide method can be used, again relies on a chemical reaction to generate an anaerobic environment, but a catalyst rather than a reducing agent
Anaerobic jars (GasPak System) are sued to incubate plates in an anaerobic atmosphere, useful if brief exposure to oxygen is not lethal
P. aeruginosa Strict aerobe
Enterococcus FacultativeGrows aerobic or anaerobic.
Bacteriodes fragilis
Culture of strict anaerobesFor culture of strict anaerobes all traces of
oxygen must be removed from medium and for many organisms sample must be kept entirely anaerobic during manipulations
Methanogenic archaea from rumen and sewage treatment plants killed by even a brief exposure to O2
Medium usually boiled during preparation and reducing agent added, stored under O2-free atmosphere
Manipulations usually carried out under a jet of O2-free N2 or N2/CO2 to exclude O2
Roll-tube (Hungate) method often used instead of conventional plates for isolation and culture of strict anaerobes