intra- cytoplasmic sperm injection (icsi)
DESCRIPTION
بسم الله الرَّحمن الرَّحيمِ لله مُلكُ السَموات وَ الأرض يَخلُقُ ما يَشآء يَهَبُ لمَن يَشآءُ إناثاً و يَهَبُ لمَن يَشآءُ الذُّكور¤ أو يُزَوّجُهم ذُكرانَاً وَ إناثاً وَ يَجعلُ مَن يَشآءُ عقيماً انَّهُ عَليمٌ قَدير¤ صَدَقَ اللهُ العَليُّ العَظيم الشورى 49-50. - PowerPoint PPT PresentationTRANSCRIPT
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د�َق� الله� الع�لُّي& الع�ظيِم َص�
50-49الشورى
Intra-Cytoplasmic Sperm Injection
(ICSI)الحقن
المجَهَّري للحيمن
داخُل البوَيضة
إعداد
د. علُّي إبَّراهيِم رحيِمكلية الطُب/ُجامعة الكوفة
IntroductionInfertility?
It is inability to conceive after one year of frequent unprotective sexual intercourse.
At the end of this lecture, I expect that you can answer the following questions:
1) What is ICSI?
2) When we have to use it?
3) What are the steps of this procedure?
Intra-Cytoplasmic Sperm Injection
(ICSI)It is, till now, the most developed modality of Assisted Reproductive Technologies (ART).
It opened widely the door to great hope in cases of sever male infertility.
The first IVF baby born by this procedure was in 1992.
In which: a single sperm injected directly into the oocyte by a micromanipulation device.
Indications:1) Severe: Oligospermia?, Asthenospermia?,
Teratospermia?
2) Crypreserved? small amount of spermatozoa (cancer patients).
3) Fertilization failure after Conventional IVF ?
4) Ejaculatory disorders ?
5) Non-Obstructive Azoospermia ?
6) Obstructive Azoospermia ?
7) Frequent failure of other modalities of treatment.
8) Unexplained infertility not respond to other options.
9) PGS ?
-Steps of ICSI- 1. Superovulation and oocyte retrieval.
2 .Oocyte preparation and assessment of quality.
Mature oocyte Normal Oocyte with granulosa cells
3 .Semen preparation and sperm choosing.
ICSI requires only one single viable spermatozoon, preferably of good morphology that is selected by the embryologist and injected in each oocyte available.
4 .micromanipulation.
5 .Incubation in CO2 incubator?
6 .Assessment of fertilization?
7 .Assessment of Embryos and Embryo Transfer (how many?).
8 .Freezing of leftover embryos.
9. Pregnancy test (success?) and fellow up.
Questions…..zzzz??
THANK YOU