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Introduction

Background of the Study

According to The FreeDictionary.com (2003-201!" iron i# chief$y im%ortant to the human &ody

 &ecau#e it i# the main con#tituent of hemog$o&in" cytochrome" and other com%onent# of re#%iratory

en'yme #y#tem#. A con#tant a$though #ma$$ intake of iron in food i# needed to re%$ace erythrocyte# that

are de#troyed in the &ody %roce##e#. o#t iron reache# the &ody in food" )here it occur# natura$$y in the

form of iron com%ound#. The#e are con*erted for u#e in the &ody &y the action of the hydroch$oric acid

 %roduced in the #tomach. Thi# acid #e%arate# the iron from the food and com&ine# )ith it in a form that i#

readi$y a##imi$a&$e &y the &ody.

Iron #u%%$ement# are mo#t often u#ed for certain ty%e# of anemia. Anemia can cau#e fatigue and

other #ym%tom# (+e&D" 200,-201!. According to ed$ine$u# (201,!" iron #u%%$ement# may &e taken

a# ca%#u$e#" ta&$et#" che)a&$e ta&$et#" and $iuid#. The mo#t common ta&$et #i'e i# 32, mg (ferrou#

#u$fate!. B$ack #too$# are a certain #ide effect )hich indicate# that the #u%%$ement i# )orking.

According to /ona (200!" many different &rand# of thi# ty%e of iron #u%%$ement are a*ai$a&$e at

a regu$ar %harmacy. The#e )ou$d &e fine if they didnt cau#e &$ack #too$#" #tomach irritation" and

con#ti%ation in mo#t %eo%$e taking them. In addition" their aor%tion into the &$ood#tream i# )eak. In

#e*ere ca#e# of diarrhea" they might ha*e a ro$e to %$ay a# a$ternati*e# to narcotic# or other toic

con#ti%ating drug#.

In thi# e%eriment" the re#earcher# )i$$ &e u#ing e%ired iron #u%%$ement# on  Allium cepa and

 Drosophila melanogaster  to #ee if mutation# )i$$ occur. According to B$amire (2000!" utation occur#

)hen a DA gene i# damaged or changed in #uch a )ay a# to a$ter the genetic me##age carried &y that

gene. utagen i# an agent of #utance that can &ring a&out a %ermanent a$teration to the %hy#ica$

com%o#ition of a DA gene #uch that the genetic me##age i# changed. According to 4rkin" 556 (201!"

fruit f$ie# ha*e a *ery #im%$e genetic #tructure" )hich make# them idea$ for genetic re#earch. It i# u#efu$ to

#tudy mutant fruit f$ie#" a# their uick re%roduction rate a$$o)# #cienti#t# to oer*e the ad*antage# and

di#ad*antage# of certain mutation#. According to *$a&.amrita.edu (2011!" the mo#t common$y u#ed root

ti%# in $a to #tudy mito#i# are onion" )heat" $enti$" &ar$ey and a$fa$fa. An onion root ti% i# a ra%id$y

gro)ing %art of the onion and thu# many ce$$# )i$$ &e in different #tage# of mito#i#. The onion root ti%#

can &e %re%ared and #ua#hed in a )ay that a$$o)# them to &e f$attened on a micro#co%ic #$ide" #o that the

chromo#ome# of indi*idua$ ce$$# can &e oer*ed ea#i$y. According to /o##i (2007!" 4nion# ha*e $arger

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chromo#ome# than mo#t %$ant# and #tain dark. The chromo#ome# are ea#i$y oer*ed through a com%ound

$ight micro#co%e.

Statement of the ro&$em

Thi# #tudy aim# to determine the mutagenic effect# of e%ired iron #u%%$ement on fruitf$y

( Drosophila melanogaster ! and the genotoic effect# of e%ired iron #u%%$ement on onion ( Allium cepa !

meri#tematic ce$$#.

S%ecifica$$y" thi# #tudy aimed to an#)er the fo$$o)ing ue#tion#8

1. +hat i# the effect of e%ired iron #u%%$ement on fruit f$y ( Drosophila melanogaster !9

2. +hat i# the effect of e%ired iron #u%%$ement on onion ( Allium cepa! meri#tematic ce$$#9

3. I# there a #ignificant difference on the mutagenic effect# of e%ired iron #u%%$ement on Fruit f$y

( Drosophila melanogaster !9

7. I# there a #ignificant difference on the genotoic effect# on onion ( Allium cepa! meri#tematic

ce$$#.

 

4&:ecti*e#

• To determine the mutagenic effect# of e%ired iron #u%%$ement on Fruitf$y ( Drosophila

melanogaster !

• To determine the genotoic effect# of e%ired iron #u%%$ement on 4nion ( Allium cepa!

eri#tematic ce$$#

• To determine any #ignificant difference on the mutagenic effect# of e%ired iron

#u%%$ement on Fruitf$y ( Drosophila melanogaster ! or genotoic effect# on onion ( Allium

cepa! meri#tematic ce$$#.