introduction
TRANSCRIPT
GEN 3040
Introduction
The fundamental of the genome project is to complete the DNA sequence for the
organism being studied and include together with the genetic and physical maps of the genome
so that genes and other interesting features can be located within the DNA sequence. Therefore,
the techniques and research strategies for the DNA sequencing had been explored by many
researchers. Sequencing is the process to determine the exact order of the nucleotides in a region
of DNA. A combination of the computer analysis and experimentation are required to find the
location of the gens and their control regions (Brown, 2006).
DNA sequencing methods include the chain termination method introduced by F.Sanger
and A.R. Coulson and chemical degradation by A.Maxam and W.Gilbert. Both of these
techniques are important in allowing DNA sequences to be determined in just a short time. For
the chain termination method, the sequencing involved the enzymatic synthesis of the strand that
complement to an existing template. Thus the DNA to be sequenced must be in single stranded
form and the region to be sequenced must be in the 3’ flanked by known sequence (Sanger,
1981). The requirements for the Sanger-Coulson sequencing include a primer that complement to
the known region to direct the synthesis of the chain, DNA polymerase, 4-deoxynucleotide
triphosphates (dNTPs) and 4-dideoxynucleotide triphosphates (ddNTPs) (Brown, 2006). Primer
is the single stranded oligonucleotides and since it complement with the DNA, the primer would
easily hybridize with the DNA (Sanger, 1981). The DNA polymerization caused the 3’-OH to
change to a –H in ddNTPs, it is unable to form a phosphodiester bond by nucleophilic attack on
the phosphate and it would cause a termination in the DNA chain. There are two forms of
labeling, the radioactive form that include the labeled primer with 32P or 33P and labeled dNTP
35S while for the non-radioactive form the primer labeled and ddNTP labeled (big dye
terminator). As a result, labeled chains with different size that could be separated by gel
electrophoresis before visualized the fragments by using autoradiograph (Brown, 2006).
Different from Sanger-Coulson method, the automatic DNA sequencing though also
carried out using the chain termination procedure, the automatic DNA sequencing involves dye-
labeled deoxy terminators in which a dye label is inserted into the DNA as well as the
terminating base. This method is much safer compared to the Sanger-Coulson since no
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radioactive involved and the automated sequencers could read up to the 96 sequences thus the
data is acquired more rapid the manual sequencing (Brown, 2006).
The computer analysis and internet databases enable the DNA to be analyzed and
studied. One popular database is from NCBI in which contain the general database that is very
useful. The program BLAST in NCBI could be used to find the homology gene that is similar to
the gene being studied. The searching of the homology gene involves the whole collection of
sequences in the database. Thus, the sequence could be compared and the origin and functions
can be investigated.The nucleotides sequence also could be translated to cDNA, mRNA and
amino acid sequence easily by using computer programs. Only short time required and the result
obtained also more accurate. With these entire databases on the internet, people also could share
their findings easily with other people around the world (NCBI, 2010).
As for molecular cloning, the process begins with the isolation and amplification of the
gene of interest. The gene of interest is then inserted into a vector, another DNA molecule that
will drive the gene into living cells to be replicated. Recombinant DNA will be ultimately
produced to be inserted into the host cell. Clones will then can be generated and the inserted
foreign DNA will also be generated. The amplified foreign DNA can be purified for further
analysis.
In this study, the DNA that has been subcloned into a plasmid from a genomic clone
promoter fragment (pOS2) of the osmotin gene from Nicotiana tabacum was prepared and
sequenced. The desired DNA fragment was purified before sent for the sequencing. The basic of
the automated DNA sequence analysis was introduced during the practical. After the sequences
were obtained, the sequences were analyzed and mapping by using those database online. By
analyzing the sequences, a lot of information of the sequences was obtained. The sequences are
obtained from the Nicotiana tabacum gene. The sequence motifs that act as the transcription
factor binding sites are also determined.
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Materials and methods
The DNA has been subcloned into a plasmid from a genomic clone promoter fragment
(pOS2) of the osmotin gene from Nicotiana tabacum. The plasmid DNA then was sent for the
automated sequencing. Then the quantification of the nucleic acids by using Nanodrop and UV
spectrophotometry were done. The absorbance at certain wavelength was recorded and the DNA
concentration was calculated. All the steps and methods in this experiment are based on the lab
manual.
The vector used was pUC118 and the restriction enzymes used were pUC118, Xbal,
SubcloneSalI, SubcloneSpeI, SubcloneBglII, SubcloneXbal and SpeI, SubcloneSalI and BglII
and SubcloneXbal and BglII. The sequence obtained (1st_Base_308861_POS_2_M13F_-20) and
(1st_Base_308862_POS_2_M13R_-20) were first edited manually by using the BioEdit
Software. Later, the edited consensus sequence that had been reversed was analyzed in the
BLAST from NCBI. The homology gene of those sequences was obtained. ClustalW was used to
find the genetic similarities between five homology genes. The sequences also analyzed at the
Expasy-Translate to convert it into amino acid sequence. The sequence motifs were identified by
using the Patmatmotif and also the Fuzznuc.
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Results
Week 1: Preparation of Plasmid DNA Template for Automated Sequencing
Initially, only sample A was observed to show pink pellet. However, as the experiment was
continued, the pellet appeared in both sample A and B. Thus, both samples were used for next
experiment.
Week 2: Quantification of Nucleic Acids Using UV Spectrophotometry
Table 1. The OD reading of of sample A and B at 230nm, 260nm and 280nm and the OD
ratio by using Nanodrop
OD reading
230nm 260nm 280nm Ratio of
OD230:OD260:OD280
Sample A 0.2537 0.6162 0.327 1: 2.4:1.3
Sample B 0.2517 0.5734 0.2959 1:2.3:1.2
Concentration obtained from the Nanodrop:
Sample A: (624.5 + 607.9)/2 = 616.2ng/µL
Sample B: (563.8 + 583)/2 = 573.4 ng/µL
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Table 2. The OD reading of two different concentration of sample A at 230nm, 260nm and
280nm and the OD ratio (UV spectrophotometer)
OD reading
230nm 260nm 280nm
999µL H2O + 1µL DNA 0.0011 -0.0140 -0.0217
998µL H2O + 2µL DNA -2.2157 -1.9648 -2.1683
Table 3. The OD reading of two different concentration of sample B at 230nm, 260nm and
280nm and the OD ratio (UV spectrophotometer)
OD reading
230nm 260nm 280nm
999µL H2O + 1µL DNA 0.0018 -0.0110 -0.0198
998µL H2O + 2µL DNA -0.0826 1.133 1.023
The concentration of the samples can be calculated by using the formula given as follows:
Concentration of nucleic acid = absorbance at OD260 x 50µg/mL x dilution factor
Table 4. The distance migrated, size of markers, and log of length of markers
Distance migrated (cm) Size of markers Log of length (bp)
0.9 23130 4.3641756
1.3 9416 3.9738664
1.6 6557 3.8167052
2.1 4361 3.6395861
3.2 2322 3.3658622
3.3 2027 3.3068537
5.2 564 2.7512791
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6.5 125 2.0969100
1.5000000 2.0000000 2.5000000 3.0000000 3.5000000 4.0000000 4.5000000 5.00000000
1
2
3
4
5
6
7
f(x) = − 2.71589442280872 x + 12.285662965085R² = 0.977684109981008
Distance migrated by band versus Log of length
Log of length
Dist
ance
mig
rate
d by
ban
d (c
m)
Figure 1. Graph of distance migrated (cm) versus log of marker (bp) of markers
From the graph, the equation obtained is: y = -2.7159x + 12.286
Thus, to calculate the length of the markers,
x = (y – 12.286)/-2.7159
Length of restriction enzyme = 10x
Table 5. Distance migrated (cm) and length of restriction enzyme (cm)
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Restriction enzyme Distance migrated (cm)
Log of length (bp) Length (bp)
pUC118, Xbal 2.6 x = (2.6 – 12.286)/-2.7159
= 3.566
3681.29
SubcloneSalI 2.6, 4.2 x = (2.6 – 12.286)/-2.7159
= 3.566
x = (4.2 – 12.286)/-2.7159
= 2.977
3681.29
948.42
SubcloneSpeI 2.1 x = (2.1 – 12.286)/-2.7159
= 3.751
5636.38
SubcloneBglII 2.5, 4.4 x = (2.5 – 12.286)/-2.7159
= 3.603
x = (4.4 – 12.286)/-2.7159
= 2.904
4008.67
801.68
SubcloneXbal and SpeI 2.2, 6.3 x = (2.2 – 12.286)/-2.7159
= 3.714
x = (6.3 – 12.286)/-2.7159
= 2.204
5176.07
159.96
SubcloneSalI and BglII 2.6, 4.4, 6.6, 6.8 x = (2.6 – 12.286)/-2.7159
= 3.566
x = (4.4 – 12.286)/-2.7159
= 2.904
x = (6.6 – 12.286)/-2.7159
= 2.094
x = (6.8 – 12.286)/-2.7159
= 2.020
3681.29
801.68
124.17
104.71
SubcloneXbal and BglII 2.6, 4.4, 6.6 x = (2.6 – 12.286)/-2.7159 3681.29
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= 3.566
x = (4.4 – 12.286)/-2.7159
= 2.904
x = (6.6 – 12.286)/-2.7159
= 2.094
801.68
124.17
Figure 3. The restriction map of the cloned insert
The consensus sequence of pOS2
>pOS2
CGCGCGAGCGCCAACGGTGTACGGGCAGTTGTTTCGGACCTCGATAGTTGCAGCATAAGTACAAGTCACAAAGGCAAGGAGGAAGAAAACAAAAGAAGATCTCAAGTATTCCATGTTTGTGGACATTTTCTTTTTAACAAGTTGCGTTGTTGGATATAGTGACAAATTATTTGGTGGTTTATATAGGAGTATGGCGGCTTTTCGCACATAGACATTAATCATATATATATTTATTAACATTTGACTAGTAAATGGATAATTCTCAAGGATGCTTTAGTAAAGGTGGAGGCGGCTAAAAGTATAAGAGCCGCCTAATAAACTAATAATTTTATGAATATAGAAGTCAATTTATTTGGTGATGATGCTGACCTATAAGCTCTGTAACGTGACACTTGTAACAATATCTCTTTTAGTCAATTTCTTGCTTTCTTTTATCTATACCATTTTTTTAATTCACAAGAAATTGGCAGATAGCCATCATTTTGAAGCTATTCAATATTAGTTGCTTTTTAACATGAAAATAAACTTAAACAAAAATATTTTTTCGCAAAAATAAGAAAAATATATAAAATTTTTATTAGAGTTTAAATATTTATCAGTTTGAACTTCATATTTCATACTCCCGAAAATTTTTATGGAGTTTGCACAGATCATAAATTTTTCATGTAGTTTTAATACTAAATCTTGGAGTTTCACTTTTAATATTGATGTTATATTGTCCCCTACTTATTTTTTCTTCCTTCGTTTTCTTTTTGAGAACTATTTCATCTATTTATATATAGCAGCTCATTAAGTAGATGGCGTTATTCTGGATGTGGAATAAACAGAGAAGAACAACACAGAAGATTAATGCCAATCGGGTAGACTTGATCAACAATTTAACAAATAAGCTGATTGTAGATAACATAAACTCATAAAGTTACGCCAATTCAATAGAGTTTGTTGAACAATTGAACTAGCATAAAATCATGCCAACTGGATAGAGTTTGTGAACACTTAACAATATAGGTGGAGTAAATACAATATAAATTCTTCCGATAGAAAAATTTCTGAAGAACTACATGGGTCCGGAGATCATTTTTTAAATATATTATAAAATGGAGACAATATTTAAAGACCAGTCCGTTATAGGCCGGCCATTAATTCTTGTCCATCAAATTTGCATCTGCTGTTGGTTTTCTGCTAGATCTACTTAAAATTTATAAAACAATCAGTTTCGAAACAAAGTCTAGTGTAGTACTACCAGAACATCAACTCATATAAAAGTCGACTCTAGAGGTCCCCGGTACCGAGCTCGAATTCGA
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Figure 2. The gel electrophoresis of the digestion products
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Figure 4. The pOS2 consensus similarities
Week 4: Collation of Sequence Data and Computer Analysis
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Figure 5. The edited sequences from BioEdit
Figure 6. The protein translation of the sequence by using BLAST program
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Figure 7. The Clustalw program to obtain the genetic relationship of the sequences
Figure 8. The Compute pI/Mw tool from Expasy
Discussion
During the plasmid preparation, the pink pellet only was observed in the sample A. However, as
the experiment was continued, the pink pellet was ultimately observed. Errors might occur
during the experiment such as the DNA might be too small or the dye was not completely bind to
the plasmid. The concentration of the nucleic acid was obtained directly from Nanodrop as the
values obtained from the UV spectrophotometer were erroneous which it gave negative optical
density readings. Thus, the values cannot be used by using the OD from the UV
spectrophotometer. It might be caused by the spectrophotometer itself, or by the sample as the
ratio calculated from the Nanodrop showed that the samples were impure.
The DNA concentration was calculated based on the concentration of a double stranded
DNA of 50µg/mL which is equivalent to an OD260 of 1. The OD of 230 as well as 280nm was
also recorded for the precaution purposes. Contamination of the samples by carbohydrates,
protein or other foreign components of the tissues will affect the OD260 readings. Thus errors
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when calculating the DNA concentration in the samples could happen. Basically, ratio of
OD230:OD260:OD280 of pure DNA sample is 1:1.8 and the accepted range of DNA sample for
further analysis is 1:1.6-2:1. However, the DNA sample obtained in this experiment for both
sample A and B were 1: 2.4:1.3 and 1:2.3:1.2 respectively. The ratios explained that the DNA
samples were not pure and rejected for further analysis. The lacks of DNA purity probably due to
the contamination during the DNA transfer from one container to others or poor centrifugation
process which caused some debris left in the supernatant. The contamination of the samples was
the reason why the OD readings were affected (Brown, 2006).
The DNA was cut by pUC118, Xbal, SubcloneSalI, SubcloneSpeI, SubcloneBglII,
SubcloneXbal and SpeI, SubcloneSalI and BglII and SubcloneXbal and BglII restriction enzymes
and separated based on the sizes by the gel electrophoresis. The lowest band on the gel was the
shorter fragment as it could travel further.
From the gel of digestion fragments, the length and size of the markers were measured. A
graph of distance migrated by the markers versus log of length of the restriction enzyme was
plotted and the equation obtained was used in order to calculate the length of the restriction
enzymes. From Figure 3, the restriction map of the cloned insert is shown. The exact location of
the insert as well as the restriction enzymes can be estimated. The similarities of the consensus
can be determined by using the online programs such as BLAST in NCBI that contains the
GenBank databases. The highest similarity obtained was 99%. The 1% unlikeliness might be due
to some of the degeneration of short fragment caused by the plasmid cutting process by
restriction enzymes.
When the BioEdit program was ran, the Restriction Map was performed as well. There
were 5 restriction enzymes detected with specific bases shown as follows:
GAATTC = EcoRI (1)
GTCGAC = AccI/HincII/SalI (1)
TCTAGA = XbaI
GAGCTC = SacI
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GGTACC = KpnI
Questions and answers
Q1. Do all regions of your sequence show exact homology to the best matching sequence you
have found in the database?
Not all regions in the sequence showed exact homology of the best matching sequence. The best
homology of the pOS sequence is the N.tabacum osmotin gene which shows 99% identical and
has 0 E value. The 1% that is incompatible with the cloned sequence probably due to the
impurities of the DNA that was sent for sequencing. There might be errors occurred during the
isolation process.
Q2. Using the BLAST output description, ‘matcher’ analyses, and the references cited, determine
the genetic relationship between the five most closely related DNA sequence and the genomic
sequence pf this gene been determined?
From BLAST, five most closely related DNA sequences of the consensus sequences pOS2 are
the N.tabacum osmotin gene, abscisic acid-activated of N.tabacum, promoter osmotin of
N.tabacum, N.tabacum ap24 gene asn Solanum phureja osmotin-like protein. The osmotin gene
is the most identical to the sequence with the 0 E-value that indicates the most significant results.
Figure 9. The pOS2 consensus similarities
>gi|1167853|emb|X95308.1| N.tabacum osmotin geneGTCGACTTTTATATGAGTTGATGTTCTGGTAGTACTACACTAGACTTTGTTTCGAAACTGATTGTTTTATAAATTTTAAGTAGATCTAGCAGAAAACCAACAGCAGATGCAAATTTGATGGACAAGAATTAATGGCCGGCCTATAACGGACTGGTCTTTAAATATTGTCTCCATTTTATAATATATTTAAAAAATGATCTCCGGACCCATGTAGTTCTTCAGAAATTTTTCTATCGGAAGAATTTATATTGTATTTACTCCACCTATATTGTTAAGTGTTCACAAACTCTATCCAGTTGGCATGATTTTATGCTAGTTCAATTGTTCAACAAACTCTATTGAATTGGCGT
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AACTTTATGAGTTTATGTTATCTACAATCAGCTTATTTGTTAAATTGTTGATCAAGTCTACCCGATTGGCATTAATCTTCTGTGTTGTTCTTCTCTGTTTCTTCCACATCCAGAATAACGCCATCTACTTAATGAGCTGCTATATATAAATAGCTGAAATGATTCTCAAAAAGAAAACGAAGGAAGAAAAAATAAGTAGGGGACAATATAACATCAATATTAAAAGTGAAACTCCAAGATTTAGTATTAAAACTACATGAAAAATTTATGATCTGTGCAAACTCCATAAAAATTTTCGGGAGTATGAAATATGAAGTTCAAACTGATAAATATTTAAACTCTAATAAAAATTTTATATATTTTTCTTATTTTTGCGAAAAATATTTTTGTTTAAGTTTATTTTCATGTTAAAAAGCAACTAATATTGAATATGCTTCAAAATGATGGCTATCTGCCAATTTCTTGTGAATTAAAAAAATGGTATAGATAAAAGAAAGCAAGAAATTGACTAAAAGAGATATTGTTACAAGTGTCACGTTACAGAGCTTATAGGTCAGCATCATCACCAAATAAATTGACTTCTATATTCATAAAATTATTAGTTTATTAGGCGGCTCTTATACTTTTAGCCGCCTCCACCTTTACTAAAGCATCCTTGAGAATTATCCATTTACTAGTCAAATGTTAATAAATATATATATGATTAATGTCTATGTGCGAAAAGCCGCCATACTCCTATATAAACCACAAATAATTTGTCACTATATCCAACAACGCAACTTGTTAAAAAGAAAATGTCCACAAACATGGAATACTTGAGATCTTCTTTTGTTTTCTTCCTCCTTGCCTTTGTGACTTGTACTTATGCTGCAACTATCGAGGTCCGAAACAACTGCCCGTACACCGTTTGGGCGGCGTCGACCCCCATAGGCGGTGGCCGACGCCTCGATCGAGGCCAAACTTGGGTGATCAATGCGCCACGAGGTACTAAAATGGCACGTATATGGGGTCGTACAAATTGTAACTTTAATGCTGCTGGTAGGGGTACATGCCAAACCGGTGACTGTGGTGGAGTCCTACAATGCACCGGGTGGGGTAAACCACCAAACACCTTGGCCGAATACGCATTGGACCAATTCAGTGGCTTAGATTTTTGGGACATTTCTTTAGTAGATGGATTCAACATACCGATGACTTTCGCCCCGACTAACCCTAGTGGAGGGAAATGCCATGCAATTCATTGCACGGCCAATATAAACGGTGAATGTCCCCGAGAACTTAGGGTTCCCGGAGGATGTAATAACCCTTGTACTACATTTGGAGGACAACAATATTGTTGCACACAAGGACCTTGTGGTCCTACATTTTTCTCAAAATTTTTCAAACAAAGATGCCCTGACGCCTATAGCTACCCACAAGATGATCCTACAAGCACTTTTACTTGCCCTGGTGGTAGTACAAATTATAAGGTTATCTTCTGTCCTAATGGTCAAGCTCACCCAAATTTCCCCTTGGAAATGCCTGGAAGTGATGAAGTGGCTAAGTAGAACGGTCATTCCTCTAATAAGATCATCTTTGGTCGAATTATTGCGTGAAAGCGTTAGTGTGGGACAATTTATTTTGTCTCGCTTTTATAGTTTACACACTTTGTTTATTTTGGTTGAAGTGGTCAAGAGATGATCT
abscisic acid-activated [Nicotiana tabacum L.=tobacco, leaf, Genomic, 3033 nt]
GenBank: S40046.1
GenBank Graphics Related Sequences
>gi|251138|gb|S40046.1| abscisic acid-activated [Nicotiana tabacum L.=tobacco, leaf, Genomic, 3033 nt]GTCGACTTTTGTATCAGTTGATGTTCTGGTAGTATTACACTAGACTTAGTTTCGTAACTGATTGTTTTATAAATTTTCCGGTAACGTCCAAATATGTCCTTACCGTCACATAGTTGGTCTATATATCCCTTTTTGAAATAGAATCCATCCAAATTATTTAGCTCTTCCGTTAATTATACTAAAATGTATGACAAACACTATTTCCTTTTTATTCAGTACTTTTTTTTCTTTATCAATTTAACTTGACAAAACTCATGAATTCCTGTTAATTTTACTATTACAGGCCCCACAGGTTCCTTCCTGACCTGAAGGAGATGAAAATTATCGGAGAAATTTTTTCGGTGGTGTTAATTGGGTGAAGGTAGGATATGATTATTCAAATTTTGAAGATCTTGTTCCTTTCAGATCTGAAGCTCCACAGTGAACAACTCCTTCAAAATCTGAATAATTATATCCTACCTTCACCAAATTAACACCACCAAAAAAATTTCTCCGACAATTTTTATCTCCTTCTTTTCAGAAGAACTAGAGCCTTCTCGGTTGGAAGGTTTTGGTGGTGCAAGTTTGATTTTTAGGAATAAAATCACGTCAAATTGGTAACGAAAATGGAGAAGGCACCGGAAATGGAGGAAACCGGATATGGGAGAATGAAAAAGGGAAAAAAAGATAAGAAAAGAAAAAAGAAGAAAGAAAAGAGAAAGGTAAAGAAAAAAAGTACTAATAAAAAGTAGATAGTGTTTGTAATACACTTTAATACAATTAAAGAAAGAGCTAATTAGTTTGGGTGGATTCATTTTTAAAAAGGGCAACTATGTGACGGTAAGGACATATATGGACCAACTATGTGTATGGTAAGGGCATATATGGACCAATTATGTGACGGTACGAGTATATATGAGCTAAAGTATTAACAAAGGGTAAATGTGCTCAATTTCGTATATTACAAAGCCATATTTGGACCTTTTTCCGTAAATTTTATGTAGATTTAGAAAAAAGCAACAACCTATAAGGGGTTGGTCTTTAAATATTGTCTTCATTTTTTAATGTACTTAAAGAATGAGCTCTGGACCTATATAGTTCTTCAGAGATTTTTCTATTGGATCGCTAGAATTTATGTTATATTTATTCTACTTTTATTGTTAAGTGTTCACAAATTTTATTCGATTAGCATGATTTTGTGCTAGTTTTATTGTTAAACAAATTTCACAGAATCGGCGTAACTTTATTTTATCTGCAATCGATGTACTTCTTAAATTGTTCATTAAATCTACCTGACTGGTATAATTTTTCTGTGTTCTTCTCTGCGCTTATTCTACATCCAGAATAACGA
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nuccore fasta 1000000 multi 5
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TATCTAATTAATGAGCTGCTATATAAATCGATGTAATAGTTCTCAAAAAGAAAATGAAGGAAGAAAAAACTATGTGGTGGGACAATATAACATCATCTATATATAAAAATTAAAGTGAAATCCAGGATTTCAGTATTAAAACTACAGGAAAAATTTATGATCGGTGCAAACTCCATAAAAAATTTCGGAAGTACAAAATGTGGAGTTCAAACTGATAAACAAACTCTAATAAATTTCTTATAATTTTTTTATATTTTTGTGACGAATATTATTGTTTGAGTTTTATTTTCACATTAAAAACTAAATATTGAATAGCTTTAAAATGATGGCTATCTGCCAAAAAGTGGCTATCTGTCAATTTCTTGCGAATTAAAAAATGGTATAGATAAAAGAAAGCAAGAAATTGACTAAAAGAGATATTGTTACAAGTGTCACGTTACAGAGATTATAGGTCAGCGTTATTACCAAATAAATTGACTTCTATATTCATAAAATAATTAATTATTAGGCGGCTCTTATGTTTAAGCGCCGCCTCCATCTTTGCCAAAGCATCCTTGAGATATATCCGTTTATTAGTCAAATGTTAATAAATATTTATGATTAATATCCATAGTACGAAAAGCCGCCATTCCCCTATATAAACCACTAAACAATTTGTCACTATATCCAACAACCCAACTTGTTAAAAAAAATGTCCAACAACATGGGCAACTTGAGATCTTCTTTTGTTTTCTTCCTCCTTGCCTTGGTGACTTATACTTATGCTGCCACTATCGAGGTCCGAAACAACTGTCCGTACACCGTTTGGGCGGCGTCGACACCCATAGGCGGTGGCCGGCGTCTCGATCGAGGCCAAACTTGGGTGATCAATGCGCCACGAGGTACTAAAATGGCACGTGTATGGGGCCGTACTAATTGTAACTTCAATGCTGCTGGTAGGGGTACGTGCCAAACCGGTGACTGTGGTGGAGTCCTACAGTGCACCGGGTGGGGTAAACCACCAAACACCTTGGCTGAATACGCTTTGGACCAATTCAGTGGTTTAGATTTCTGGGACATTTCTTTAGTTGATGGATTCAACATTCCGATGACTTTCGCCCCGACTAACCCTAGTGGAGGGAAATGCCATGCAATTCATTGTACGGCTAATATAAACGGCGAATGTCCCCGCGAACTTAGGGTTCCCGGAGGATGTAATAACCCTTGTACTACATTCGGAGGACAACAATATTGTTGCACACAAGGACCTTGTGGTCCTACATTTTTCTCAAAATTTTTCAAACAAAGATGCCCTGATGCCTATAGCTACCCACAAGATGATCCTACTAGCACTTTTACTTGCCCTGGTGGTAGTACAAATTATAGGGTTATCTTTTGTCCTAATGGTCAAGCTCACCCAAATTTTCCCTTGGAAATGCCTGGAAGTGATGAAGTGGCTAAGTAGAGTGGCTATTTCTGTAATAAGATCACCTTTTGGTCAAATTATTCTATCGACACGTTAGTAAGACAATCTATTTGACTCGTTTTTATAGTTACGTACTTTGTTTGAAGTGATCAAGTCATGATCTTTGCTGTAATAAACCTAAGACCTGAATAAGAGTCACATATGTATTTTTGTCTTGATGTTATATAGATCAATAATGCATTTGGATTATCGTTTTTATATTGTTTTTCTTTTGAAGTTTTAGTAAAGTCTTAAGCTT
osmotin {promoter} [Nicotiana tabacum=tobacco, Genomic, 2051 nt]
GenBank: S68111.1
>gi|545027|gb|S68111.1| osmotin {promoter} [Nicotiana tabacum=tobacco, Genomic, 2051 nt]GTCGACTTTTGTATCAGTTGATGTTCTGGTAGTATTACACTAGACTTAGTTTCGTAACTGATTGTTTTATAAATTTTCCGGTAACGTCCAAATATGTCCTTACCGTCACATAGTTGGTCTATATATCCCTTTTTGAAATAGAATCCATCCAAATTATTTAGCTCTTCCGTTAATTATACTAAAATGTATGACAAACACTATTTCCTTTTTATTCAGTACTTTTTTTTCTTTATCAATTTAACTTGACAAAACTCATGAATTCCTGTTAATTTTACTATTACAGGCCCCACAGGTTCCTTCCTGACCTCAAGGAGATGAAAATTATCGGAGAAATTTTTTCGGTGGTGTTAATTGGGTGAAGGTAGGATATGATTATTCAAATTTTGAAGATCTTGTTCCTTTCAGATCTGAAGCTCCACAGTGAACAACTCCTTCAAAATCTGAATAATTATATCCTACCTTCACCAAATTAACACCACCAAAAAAATTTCTCCGACAATTTTTATCTCCTTCTTTTCAGAAGAACTAGAGCCTTCTCGGTTGGAAGGTTTTGGTGGTGCAAGTTTGATTTTTAGGAATAAAATCACGTCAAATTGGTAACGAAAATGGAGAAGGCACCGGAAATGGAGGAAACCGGATATGGGAGAATGAAAAAGGGAAAAAAAGATAAGAAAAGAAAAAAGAAGAAAGAAAAGAGAAAGGTAAAGAAAAAAAGTACTAATAAAAAGTAGATAGTGTTTGTAATACACTTTAATACAATTAAAGAAAGAGCTAATTAGTTTGGGTGGATTCATTTTTAAAAAGGGCAACTATGTGACGGTAAGGACATATATGGACCAACTATGTGTATGGTAAGGGCATATATGGACCAATTATGTGACGGTACGAGTATATATGAGCTAAAGTATTAACAAAGGGTAAATGTGCTCAATTTCGTATATTACAAAGCCATATTTGGACCTTTTTCCGTAAATTTTATGTAGATTTAGAAAAAAGCAACAACCTATAAGGGGTTGGTCTTTAAATATTGTCTTCATTTTTTAATGTACTTAAAGAATGAGCTCTGGACCTATATAGTTCTTCAGAGATTTTTCTATTGGATCGCTAGAATTTATGTTATATTTATTCTACTTTTATTGTTAAGTGTTCACAAATTTTATTCGATTAGCATGATTTTGTGCTAGTTTTATTGTTAAACAAATTTCACAGAATCGGCGTAACTTTATTTTATCTGCAATCGATGTACTTCTTAAATTGTTCATTAAATCTACCTGACTGGTATAATTTTTCTGTGTTCTTCTCTGCGCTTATTCTACATCCAGAATAACGATATCTAATTAATGAGCTGCTATATAAATCGATGTAATAGTTCTCAAAAAGAAAATGAAGGAAGAAAAAACTATGTGGTGGGACAATATAACATCATCTATATATAAAAATTAAAGTGAAATCCAGGATTTCAGTATTAAA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 16
GEN 3040
ACTACAGGAAAAATTTATGATCGGTGCAAACTCCATAAAAAATTTCGGAAGTACAAAATGTGGAGTTCAAACTGATAAACAAACTCTAATAAATTTCTTATAATTTTTTTATATTTTTGTGACGAATATTATTGTTTGAGTTTTATTTTCACATTAAAAACTAAATATTGAATAGCTTTAAAATGATGGCTATCTGCCAAAAAGTGGCTATCTGTCAATTTCTTGCGAATTAAAAAATGGTATAGATAAAAGAAAGCAAGAAATTGACTAAAAGAGATATTGTTACAAGTGTCACGTTACAGAGATTATAGGTCAGCGTTATTACCAAATAAATTGACTTCTATATTCATAAAATAATTAATTATTAGGCGGCTCTTATGTTTAAGCGCCGCCTCCATCTTTGCCAAAGCATCCTTGAGATATATCCGTTTATTAGTCAAATGTTAATAAATATTTATGATTAATATCCATAGTACGAAAAGCCGCCATTCCCCTATATAAACCACTAAACAATTTGTCACTATATCCAACAACCCAACTTGTTAAAAAAAATGTCCAACAACATGGGCAACTTGAGATCT
N.tabacum ap24 gene
GenBank: X65701.1
GenBank Graphics Related Sequences
>gi|19782|emb|X65701.1| N.tabacum ap24 geneATATTATTGTTTGAGTTTTATTTTCACATTAAAAACTAAATATTGAATAGCTTTAAAATGATGGCTATCTGCCAAAAAGTGGCTATCTGTCAATTTCTTGCGAATTAAAAAATGGTATAGATAAAAGAAAGCAAGAAATTGACTAAAAGAGATATTGTTACAAGTGTCACGTTACAGAGATTATAGGTCAGCGTTATTACCAAATAAATTGACTTCTATATTCATAAAATAATTAATTATTAGGCGGCTCTTATGTTTAAGCGCCGCCTCCATCTTTGCCAAAGCATCCTTGAGATATATCCGTTTATTAGTCAAATGTTAATAAATATTTATGATTAATATCCATAGTACGAAAAGCCGCCATTCCCCTATATAAACCACTAAACAATTTGTCACTATATCCAACAACCCAACTTGTTAAAAAAAATGTCCAACAACATGGGCAACTTGAGATCTTCTTTTGTTTTCTTCCTCCTTGCCTTGGTGACTTATACTTATGCTGCCACTATCGAGGTCCGAAACAACTGTCCGTACACCGTTTGGGCGGCGTCGACACCCATAGGCGGTGGCCGGCGTCTCGATCGAGGCCAAACTTGGGTGATCAATGCGCCACGAGGTACTAAAATGGCACGTGTATGGGGCCGTACTAATTGTAACTTCAATGCTGCTGGTAGGGGTACGTGCCAAACCGGTGACTGTGGTGGAGTCCTACAGTGCACCGGGTGGGGTAAACCACCAAACACCTTGGCTGAATACGCTTTGGACCAATTCAGTGGTTTAGATTTCTGGGACATTTCTTTAGTTGATGGATTCAACATTCCGATGACTTTCGCCCCGACTAACCCTAGTGGAGGGAAATGCCATGCAATTCATTGTACGGCTAATATAAACGGCGAATGTCCCCGCGAACTTAGGGTTCCCGGAGGATGTAATAACCCTTGTACTACATTCGGAGGACAACAATATTGTTGCACACAAGGACCTTGTGGTCCTACATTTTTCTCAAAATTTTTCAAACAAAGATGCCCTGATGCCTATAGCTACCCACAAGATGATCCTACTAGCACTTTTACTTGCCCTGGTGGTAGTACAAATTATAGGGTTATCTTTTGTCCTAATGGTCAAGCTCACCCAAATTTTCCCTTGGAAATGCCTGGAAGTGATGAAGTGGCTAAGTAGAGTGGCTATTTCTGTAATAAGATCACCTTTTGGTCAAATTATTCTATCGACACGTTAGTGTAAGACAATCTATTTGACTCGTTTTTATAGTTACGTACTTTGTTTGAAGTGATCAAGTCATGATCTTTGCTGTAATAAACCTAAGACCTGAATAAGAGTCACATATGTATTTTTGTCTTGATGTTATATAGATCAATAATGCATTTGGATTATCGTTTTTATATTGTTTTTCTTTTGAAGTTTTAGTAAAGTCTTAAGCTTAAACCAATAAACAAAACAAACAAAAAAAAAGGTATTGATAATATTCTAGCATAGGACCCAAGACGTTGAAAGTATAAAATGGTAGGATAAGATAAACTATTTGGAGCTC
5.
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 17
GEN 3040
Solanum lycopersicum DNA, chromosome 8, clone: C08SLe0023D06, complete sequence
GenBank: AP009601.1
GenBank Graphics Related Sequences
>gi|165263516|dbj|AP009601.1| Solanum lycopersicum DNA, chromosome 8, clone: C08SLe0023D06, complete sequenceGAATTCTTCATATAATTAACTTCTTGATCATAATTTGCGACTAATTAAGCCACAAATTGTCACTAATGCCATAATTAACGATTGATCAACTTGGGGTTCCACTTCTAGAGTTAACACATCATCTCCATATGCAAATCCTCTCGACGATTGCTTTTGTTTTAACTGCGACAAAAAAAAAATCAATATCAGAATCTTTATACTATCAGTATATATAACTTGGATTAAATTTTTTTTTTAAAAAAAACTTTATATGAATTTATAATTTTTAAAGGACCTACCTCAGCAACAATTTCTCCAGTACTATTTGTAACTTTAAATGATGATTTCTTGTCCAATTTTTGAATCTTATAACAACTTTCAATTGATTTTTCATATCCCAAATTCACATTACATATAACATTTCCTCTTGAAAATGTGCAATTCTTTTTTACTTGAAACCATGGCCTTCCTTTAAATCCACCACATAAATATCCATTCCATCTACCAAAAACTCTCAATTTCTGCAATTAACATAAAAACAAAAAAAATAATTAATTTCAACTTCCATAACATAAAATATACTCATTCTGTCTCAATTTATGTATTAATACTGTTCGAATTTCGAGAGTTAAACAGATCCTATGTTTGAATTCATAGTCAAAGTTTCTGGAAATTCAAACTGTGTCACATAAATTATAACGGAATAAAATAATTTATTACCTCTTTTTTGATGGAAAAGAGAACTTGTCCATTAAGATCCATAAGAAAAACTTCATTGCTACATCTTTCTTGATAATTATCAACTCTAAAACAAAGTTCACCTTTGGAATTAAAAACAGTACATCCATTTCCATGAAAAACTAGTGATTTCATCCATATTGTAAATATTTCTTTATTAGATGTAACATAAGGAGATGAACAAGAAGAAGAAGAAGAAGGGGAAGATATATTTTCTGGATGAATTTTACCCATAATTAAAGTAAAATTAAATTTATAAAAAAAAAAATTGTAATTTTGAAGAAATGAAATCGCTTGAGAGAAGATTTTGGAATTTGATTTTTGGTGTGATGAGTAATAGAGAAGTTGAAAGGGTATAAATAGAAGTTAAAGAAACAACCACTGAAAAAAATAGTTAAAGAAATAATCAAGAAAGTAGAAACATTTGACTTTATAAAAACGTGTAATGTTGACGAAGTATTTTTTTTTATTATCATCACCATTTAATATTTTGATATTTACTAATTCGATTATTTTAAATTCGTGTAACGCAAAATTTATTACAGAGAAAATTATTTTTTATTAGAAGTTTTTTTATTATATATAGGCTTGAATTCGAGACTTCTAATAAAAAATAGAGAAATTTAATTCATTATACAGTGATAAAACTACGTATATAAGAGATTGAGTTGATCGATTGATATTTTTCACTGAAAAATCACATTATATATATAAAAATATGATTATTTTATACATTTATCTTTTCATATTTTTGATATTTCTCGATAAAACTCTTTTGATATTAAATGATAGGCATTGATATATAGAAAAATAATAATAATAATGGTTATACTTAAGTTTTTTATTTTACACATTCCATATTATTGTAAGAAAACAAGTCAATTTAATAAAAAATTACTTATTTGAGATAATTTCAAAATAATATTGTGTCAAAGTCAGTTTAAATAAAAAAAATATTTATTCAAAGATAATTTCAAAATATTATATCAAAACTTATACATAAGAAGACATAGACAAAGAGGTTTTGTCTTTATTTATTCACATCCAGTCTGTCTTTGGGCAAATAAATAAAGTAGAATATGACTCTTCATAGAGTTTATTTTTATTCTTATAATATGTGTGTCTTTGTATACCCTATATATAATATGCTTTTTTTTTGCCATGTGAGGTGGCCTTATGGAGAACCACTAAACCACAAATCATCACAACATAGATTCATATTTCTCCTTTATATCCTCCTTCACCTAGGAACTAAAATATATTCTTTTTATAGTTTTGTGTCAAAAGATTTTTTTTTTAAAAAAGTTCCAAAATGTGGTCTATAATAACTCACTAGGCAATATATCAAAATCTTGGAGGTTCACCGTGGAAAAATATAGTGGGTCGAAACTTATCCATTTTAATCAGATATTTAGAGTTTGATTTTTTAAAATGAGAAAATTTATATTAGAAAGTGTTTTCTTTTTTTAAAAAAAAAAAACTTTTATGCAACGTGAATATAAATTAATGTTGTAGTGAACCCTTAAGTTGTAGACAAATGTGTGAAAGTATCAAACTGATTGACAATATGAGTTTGCTATCTTTTTATACGATTTGAAATTATTAAGTTTTTAAGTTAAATTAAATTTACGATTTATTTTCTTGATATATTAAAAGAGTGAATATGTTATCCACGCTTCAAATGTACATGTTTTCTTCTATGATTTTTGCCCAAATTTTGAATTACACAACATTGGGCTCCAAGATATATTCTTTCACGTTTCAAATGTCCATTTAATTTCTTAATTATAACATGATATTTTCATATTAGTCAGAATTTTAGTGATTTTAATAATCATTATGACTTTTTTAGATTATTAATATTTGTAATTCATAAGTCAACTCCTATTTTCTTAGTGACATATTAATCCCAAATCAGTGGGGTTCCACTTACGTTTGTGTAACAACCACTAAATACTTCTTCAAAAAAAAAAGAAAAAAAAAAGAAAGTGGAAGTGTAATAACTGTTCAATGTCAAAAGAGACACAATTAAATTAGAGATTTTAATATATGCACTTAAGGGTAAATGAAATTAAAATAAAATGGTGTCAAAAGAAAGGATTATCATTGATTAATTTAATAAATAGTTGTTGTTAATTGTTTGTGTGCATCGTTTTGCGTGCTTATCCCTTAGATGAGGGAAAATTAGGTATTTGACACTGACTAACTGTTTATTTTATATTTTATATAAATTTGAGATGGTAAAAAATGTAATTTTAATATAAATACAAAATTCAAGAAATTATAATTCTACAAATATACCCACCCTTAGATATCAATAAATTTTATAACAGAATGGTAATATTCAAATATGTTCCTTTTGATGATAGATTTCTTTTTATTTTTTAATAATTTAAACTCGAGATTTCTAGGCCGTAATAGTTACGATCAAAAACGAGTAAACTCACACATAAAAAAAGTTGTTGATGATGATTGTCCACTGGGAATTTTGCATCATGAAAAATTTAAAGTTAAGAGCTGGTAAAAATGACAACCATATTCTTCTTTTAGTGGAATTATAATAAAGACAGGCA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 18
GEN 3040
CCACCAGTAACATATTCTCGACTTATATATAGGATGTGACATTTGATAATATTCTTTTAGTAAATTTTAAGTTTAGCAAATATTAAAATTATATGTATATGTTATAATTATAATTTGTATAATTACATGAAGTGTCAGATCTTTATAAATTATTGGTAAGTCTTTCGTTTGTATAAAAGCGCAACGATTATATATATATAGGTTAGACAATTGTACATACGTAATTACTATGTACATATACCAATGATTGTGTTTGTATATATGTATAAAAATGAATTTGTATATAATTGAATCGAAATAAAATATTTATATATCACATCTCTGGATTTATACAACACAATTATACAAATTGTAATTTGTATAATTTGTGTTTGTATAAGAGAAAGACAAAAGAGAACTGGTAGAAGAAGATCTGTATTTGTATAATTATAAATTTATAGGATAAAAATACATGTATTTGTATGTGTATATACAATTTTAGTTCGCTTTACACAAACGCAAACGCAATATATAGGCGTGGAAGAGCGGCGAGTGAAATTCTTTGGAGAGAGAGGCAAATGACAAGAGTTTGATATGAATTACAATTAAATGAAAAGTATAATTATAACATTAAATTTGAACTAATAATTTGCTATTTTATATAATTTTTCCTAATTTTAAAGTTAATGAGTTGTAAAAGAAAAAGATATGTTTATCGTAAATAATTCAGAAAGAAAGTATGAAACAAATAAATTTCAACGAAGAGAATAATAAAAAATCGGGAAAAAAATATATTATCCTTTTAAGGGATTTTTCTAATATTGGACGGCAAACTCCACTATGTACCGACAAGCAAAGTTAATTTGATTAGAAATTATGATGAATTTGACCTTAATTTGATATTATTATTATTATTATTATTATATAACACTGAAACTAGACTAAGGTGAAAAGTGTCATGATATGTAATAATTTAGAAGCTTGAATCTGTCATAGTTCTCAAACATATATATTTTATTTGTTTCATAATTTACTTTTTTCTTTCACGATCAAATCGTTTTAGTTTGTACATAAAATTTATATATTTGTAAATTATATAAAATATCTTAAATCATAAGTGATAATTCAAAATATTTAAAAGATATATAAAAAAAAATTATGATAAAAATAAATTTATTTGAATCTAAAAAGCTAAAAGGTACCACATAAGAAATATGAACTTCTTGTTTACAAATATTTAGAAAAAAATTAACAAGGTTTTATATATTAATTTAAAAACTTTCTTGTTAGAGTTTACCATAAATATCTATGCGAATATCTTTTTATAATCACATAAATATTATGACATGACTAAAATTATATTTTTTTCTTATAATTATACAAAAGTTATAACATGTCCAATATTACGAGATAAAAAATATCTTCTCTATTTTAGACTACATTTAAATAAAATTTACATCTCATATATTAATTAAAACTATATATAGGGGAGTATTATTCTTAACACTGTTTTCTAAAAAAAAATTTGGGGAGAGTTTTGCGGAGGGGTGTATGAAAAATGTTTGAAAATGCAAATTGAAAGACATATTCATAAGGTGTGAATCTTAGAATTGGGACGTACGCTTTATTTTTCTTTTCTTTTTTATAGTTTTTGAACCTTTTTTTGCTTTTAATGATATAATGACATTTCTCAAATAGTGATTATAATACTTCTTTTTTTGTTATTGATTTATTAATATTTTTCTTAAATAAAAATTATATTATATTTTTCTATATATTAGAGGTTATTCATGAAAGATTACTTGAAAATAATTAAAAAATTTATTTTGCATATTTTCTTAAGAATAAAACTATAAAATTGAATATACATGAGATTATTTCCCGTAGATCTATAAGACTTCATTCTGTATTTTAAAAGTTTTATCTCGTTTCATACTGCATAAAACGTCTCATCTCACCTTTCATCATATAAATTATTATTTCTCTAGATTTCATTATCAAATAATTATATGAATTTCTCAATTATTCAATATATATTTTTAGTTAATGGTATAAAATATGTGATCATATTTAATTATCGTAACATGACAGAATAACAGTCGGATGATGGACGTGGCATGTTTTTGAACTAAAACTTATCACATGTTAACTTTTAAAAAAGTTGATCCAAATGCTTAAAATATGATTGGATAATATATATAGGACAATGAGTAAATTTATAATTTTTAGGATAATTTGTATATAAATAAAATAAATTATAAGTGCGGGTTGATGCATGCACACCAAAAAGTTTGAGTTTGACAGAGCCATTTATATTTTTATAATAAAATTATAATTCATCACTATGATTAGAACAAATTAAATATCCACTTAGGGTAATTTTTTAATTTTTTTTGGAAATAAATAATTAGTAGTGGTGCTAATATCTATTGAAGAAATTTAGGTTGTCTTTTTTACTTATTCTTGATAACGTATTAATGTGCATGCTGACATTGGTGAACTTAATTACGTAGGAAATCATTTTTTTAATGTTTACAATGGTTTAATGTTAATTTAGTCATAATGTCCTAGTAAGCAAGCATTTACTTTACAACCTGTTTATAATTACCTAATTGCTTAAACCATCAAGCATACCATTTTGAACACCACTAACTTCTTTCCTTTTTCGTTTTCGGTGTTCGATATTTATATTGTAGTCTGATTAAGTTAGAACTTTATATTTATACTTTTTGCATTCGATGTTTAACGATTATATTGAAGTTTGATTAATTAGTAAATTTAAATTTGCACATTATCAAATACACTCGATATTAGATAACCACATTGCAGTCTGATTAATTTGATTTCGTACATTACATCGTCCATTTCTTAAGGTGACGCTTTCATTTTCTCCCTATTTAAAAGGGACAATTTAATGAGGGAGTCAACCATCGATAAATCGTAAATTGAAATTGATCATACTCTGATATCATGTTAGAAATGAATCTTGAACCGAACTCAATAAAAAGAAAAAAAAAGTCCACAAAGTGAATATTGTTCATATAAGGGAGACCAAATCTCTTATTCCTACTCAATGTGGGACGGTCAACAAAAACATAGAGACTTTCGAATATTAGTGAAGAAATTTTAACCATCCTAACATAAGTCATATTTTTTAAAAAGCAAATGCCACTTATATTAGAAATTACCTTATTCGTTAGGCCACGTACAATACACCATAACTCACGTTGGTGTGTCGAACAACATAATTGATATTTGACTATTTTATTATCTATATATTCAAACATCAGCAGTTATTCAAATCTTTTTGGAACATCAAATTTTCAAAAGTAAATAAAAAGTAACTTAAAAAGTGTAGATAGACAAATTCAACTTGAAATATATACGAGAAAAATAATTTAGTTGCTGATGCTCAACTTAAAACAAGAAAAAATGAGCTCATTGGTAATCATGAACCTAAAATAATATCAACAAGGAATACGTTTGAAGTAATTTATCGTATGTGATTTGGATAATTATTCCTTTATTAATTGTTAATTAGGATGATGTGTTTTTGTTCCTAGTATATTAAAATAATCAAATTTATTTCACAGTTTTATATAAAATTTTAAAAAAAGAAGACATCATCTTAATTCTTAGCTACATCGTCTTTTCATGAACAAATTTGATGATCTAGTAATATTTATATAAAGAAATAATTTAAAGTAAATAACAATTTTTTGGATATAAATTATCAAATGTTGTAAATATTGTCATTACAATATATATAAACAAGGATATTTTAATAGTTAGGTTGTCTCACCAGGTCCACCATATAAAGCGACGTGCTCAAATTTTCCTTTCGTTGGTGGAATATCGTAAACCCTATAAAAATCGGGATTATCAGTGTGCGGGATCGTATTTATCATTATCGTCTCACCTCTATAGTTTAATGCAATAATTTTATTGCATGAACCATCGTACCCTTGCTTTTTAGTGGGGAAAAAACTTGAACCCATTGGTGGTTCAGGT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 19
GEN 3040
ATGTTTTGTGGGCTATATGCTACTCCTCCCCATTCTATGTGTGTAGCAAAGCTAGCTAACTGAGTGAAGATGTTTTGAGGCCAAAATCCAATTTCTCTATGCGTTTCTTCAACCAAAACCCACCAATTTCCGTTGGCTAGATCCTTTTATTTCGAAAAAAAAAAAGATATATATATATATATATATTAGTATCTCATTTTATATTCATTTTTTTTTTAAAAAAAAAAACAGTTCTTCAAAAGACAAACATAACATATACCCGATCGAGGTACATTGAATATTCCCACAAGTTATGAATATCACCACGTTGTGAAACATTACTAAATGGCGCTCCAATGCCTATCTCAGTGTCTACCAGTACAAATCCAGGACATAGTGTATCAAAGCAACCTATATTACCAGCCTATATATAGACAGTATTAAAAAATTTTATGAACGCGAAATAAGAGTTAACAAAATATTCATAAAAATATCAACTAACCTGAAAATGTATGAACATTCTAGTTAGGGTGTCGCCATATAATGTTGGATCCACCTACGTACACAAAGAAATAGAACTATCATCATGATAAATTGAATGGTTATACACTTTCTAATCTTGCATTATATTCGAGAGGTGTCATATTCAACCCTCTAAATACGAGATAATTTATTTATAACAAAATAAGCTATAGACAAAAGCTTACTCTCCAACCGATTTGTATAATGTCCGATGATTTTTGAACTTTCAATCGACATCCACTGTGTTGTGGAGCTTTAACAGAAGGATTCCATAAAGTAGATGTCATTCCAGCTCCCCCAAACTTGTAATCCAAATCATCACGAGTTGAAACTATTGCAACCTTCAATATTTTTATAAATTCTTTTTATTAAGAAAAAAAAAGACATCAAAAAAAAATTTAGAAAATTAAAAGTTCATATACCCTATATCCTTGTGTAGATAATAATCTTCTTTTCAATCCACTGTGATTCCTGGTCTTTGGAAAAGTAATAAATAAAAACAAATGAGATTTGATTTTAATGGTGAAAACTAAACATCCTATACGTTTATTTATATTAATATAAATTATATATATAATTTAAGATCCTATTGATGGAAATGAATTATTAATGATACATCAATAATTAAATTGATTTGACATTATCTTACCTGAGAAAATTGGGTATGAACTTCTGGTGGTGGCATATGTTTTTGTCTGATAAGATCATCTTTTGTAATTCTTTTAATCGGAACTGATCCAAAAGGACAACCACCATCATTTAGCCATATCGTCGATGACTCTTTAGACTTTGAAGTACCTGAAGATTGCTGTATTCTAGATAACGTAGGCTTCATCTATTCACTCAAAATTAAAATTCTAAATCAATACATATATAAATCCTTGTTAAAGGTCGATAGTAAAATATAATTTGGAACGACATACATTTGGATGAAAATTGTGGTTCTTCAAAGATGGATGATCAAACGCAGGTTGCTTGTAAAAATCCACACAATCATATATATCTCCATATGTAGTCTGCGACTCAAAATTTAAATAAATAAGTCATTTAGGTTATAACAAATGCATATATATTGTTATTCAAATCCTAATATGAAAAAATACCGTAATTGTTTTGATTGCAGGCTTGTTCAAAAGTTTAAGTTGACTTTCTAACTCTTGATCCTCTAATTCGGATAAACTTATTTCTCCTTGAACCTCATTATAACTCAGAATAAAATATAACACTAAAATTTGTTGAATAATTCCTTGATTTATCTGCATTGCCTGAACTTTGAGAGAGCATATGTTAAATAGATTTAGAGAGGAGATTAAAAAAAAAATTGTTATGCAAGTTAAGTAATGTAATTTGTTTTAAAAGCAAGATTATCTTAATTTTGTTAAGAATTTATTATATTAAATGCAAGTTGACTGTTTGCTTTAAACTTTAATCTACAAAACAGCTGTATCATTTGACCACTGAGAATATTATCTCATTTTTAAAAAAAGGCAAACTCAATTTATCCTGATGTATTGTTTTTTCTTTTATTTCAATTTAAAAGGTTGATGTGTTTCTTACAAGCCATAACAATAATAATATTTCATTATATTTAATATAATTTTATAATAATTGATATATTTTGGTTTTGTGTTTTATGTTAGTATATATCAAGACATAACACCAAATTAAAAATTATTCACCTATATGAATATATCAACCATCAAAAAATTATATGAACAAGCGATTTCGATTTACGTATTATTTTCTATAATTATTCTTTAAAAGAAATTACATGATAGCGAGATTTTGCAAGTGTATAACATATATACAGTTATACATGTATCTGTATATCTAAAAAGTTATAATTGACATTACTCTCACATACCTCCGCGTGATTTACAATAAATATGGGATAATATTAAGCCACTAATTATTTTATTTTATTTTTTGCAAAAATGGACGCTAATAACACTATTTACTAAATGTTGTCACAATCAAAATATTAATACAAATAATCCATAATTCCACCAGTCTAAAGTTTGTCTTTCCACTTAAATTAACGGGCCTAAGATTTTGCCCTCCCAGCCCAAAAAGCTTGAAAAGCCCAGACAAAGTCTAGAAAAATAAAGCCCAATAGCTGTTGACATTAGGTAAATTGTGACTTCAGTGGACGATTATTTATATTAAGGGAAAATTGTATATAATAGCAAATTAATAACCTAAAATAAATGGAGTAGCTAGGGTTTGATTTAATTGTGCTCCATAGCAAACATTTGCAAAAAATTGTCAGGCGCCTCTCTTTCAAATATCTCGCTCGCCACTCTCCTCCAGTTTCTCGCTCGCTTCCTCACTTTTTATACAAACACGAGTGTATAAAAATTGTTTCTAATTGTATAAAGCAGAGAAAATTGTATAAATACATATATTTTTGTTCCCCTCTCTCCCCTCTGCCAGATCTCGCTCGCCACTCTCCCAAATCTCGCTCGCCACCCTCGCCTTTCTCACTTATACAAACAAGAACCGAAATGTATAAATTGCATTTCTGTTTGTATAAAACGTGAAAAAATTGTATGTACACATGCAAGTACATATATTTTCGTCCTATACATTTATAGTTATACAATAAAAGTACTCCCTGCCCAGTTTCTTAGCGACAAATGGTTAAAATTGTATGTGCCATCCTAAATTCTCGCAAATTTCTTGCTTCTCCATTCATTTTTTTTAAGCAAATGGTTAAAATTATTAGACAAAAATAGTAAATGATCTAAACTTTTCCTTTTTCTTTTCTATCTTTGTAACTTTTAGGGAATAGAGATTGAAATCCTGGCTATATAGATTTTTTTTAATATATATTTGATAGCTCAAAAGGATTTAAAAATCACAATTCTCATCTAGACTTTGAAATATTTTTTTTTTTTAAAAATGGCATTTACAGGGGCCCCACAAAAATGTAAAGTATGTGAAAAAACTGTTTATATTGCAGAAATGATCTCTACAAGCGGTGTTGCTTATCATAATACATGTTTTAGGTGTAACCATTGCAATGGAAGGCTTGCAGTAAGATCTTTTCATTTAATAATTATATCATCTTTTTATATATAAATTATAACAAAATATAATCATGAAATGTATAGGTTATCTATAGTTCTTAAAACAATTTATTTCATCAACATTAATTAATCTTTTTATAATACATTTTGATTAATCTTTTTTTTTTAAAAAAAAAATAAAAATTTGCAGTTGAGCACTTATTCAACTCTTGATGGAGTATTATATTGTAAACCTCATTTTGAACAAATCTACAAGGAAAAAGGAGGAGCTCCCTTGAAGCATTCTGCATCATGTAATTTTATTTTAATTAATACACTTTAAAATAATTAATCCTACAATTAATTTTAATTATCTGATTATTTATTTGTTTCCTTATTTCTTGAGCAGTGGGAAGGCAAAATGAACTGGTATGCATCATTTATTTTACATTCTCTAAAATTATTTTTACAATCAGAATTA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 20
GEN 3040
TGAGCTAAAATATTTGATAGACGTCCAAACGAAGTTAGTATTTTTTGTTTAAATTCATTAAATATGTATAAATATTGAATTATGAACCCAATAACTAAAACGAGCCATGAGTCAACGACGTATTTAGAATTATAAACTTTAAATTCTGACTTTGCCTGCGTTTATATTAATTTTCTGTATAATTTTATTGTTATTAACAGAACAGAAGCTCTAGTAAAGTTTCGGCTTTATTTTCTGGTACTCAAGACAAATGTGCAGCTTGTAAAAAGACTGTTTACCCCTTGGAGAAGGTTACTATTTTTACTTTTATTTTATTAATTTCTTTTTACAATTAATAAATATCAACAAATTTCTTCTTAAAATTAAGACTAATTCATGTCAACAATTTTTTTTTTTAGTTTTTCTCTAGTCTTATAAATGTACGGAAGTAATTTGTTGTTTTGGAAAAAAAGAAAAAACTAATTTCCACATTTTGTTCATGCTAATAAAATTAACAACATATCTCCAAGTTTTATTATCTCGGTTAATTCTGATTTTGATTTTTGTGATATATAAAACATAATATCTTTAATTTTTAATTTATTAAAATGTGTATCTTTAGTTCATTTACATATCGGGAATATTTCATATCTGAACTATTTTCAAAAACAATTTACCCTGCTCATCATCTGATTTTATATAGAAAGACTAATTTTATTCGGACATTGATATAGCGACAATATTTTTAGTGAAACTTAAGAAAAAGAGAGATTCCTGATCAACTTATTGATGTTATGATTTATCTTAATATCGAAGTAAATCAATCAAAAGTATATATTTCAGGTAATTAAAGGACTAAATTGTAATTTACCAATAATCTAGGAGCTAAGAGTGTATGAAACGGATGTATGAGATAACGTTCTCCGCTTACTTAAAATCTTGAATCTGCTTCTGAATAAATATTTTTTTTCAGGTGACGGTGGACGGTGAAATGTACCACAAGATATGTTTTAGATGTGTCCATGGAGGTTGTAAACTTACAACATCATCTTATGCTGCATTTGATGGAGGTCTCTATTGCAAACCTCATTTCTCTCAATTGTTCAAAGAAAAAGGTTCATATAATCATCTAAGCAAAAATGCTTCAATGAAGAAAAATGAAAGTTTAATCAACGTTGAACAAGAAGGTTCTAGTACAAACACAAGATGATAAGTCATCCTATGTTAATTTACGTATGAAATTTTATTTAACGTCAATACGTTTATATTCAGATAATTGTAAAGGGTTATGTTCACTCATATGGTAGAATGACTAAGTAAACATGATAAGAAAAAAAATATTGTTTGGTAGTTCACTTATTTAGAAATGCATGGTAGAAAATAAATATATTAGAGTAAGAAGTTTACTAATTTTTTCCTATAAAAACATAATATATTTTTATTTTCTTATCGATTTAATCAAACTTCGATACAAATAACTATCGAATATTTTTCAGTTTGAAAATTTAATAAAATGATGCAAATAGTATGACATAAATAAAATTAGGATCTACTTTATTTTTTATATTTAAAAAAGTGAATCTATTCTTATGCCTTTTTTCTGATGGCAACCCCACCAAAAAAGTGTTCAAATAATAATTGGTGTTTGTCTTAGCATTGTTGAGAACTGCCAAATCCAATATTTTTTTTGATAAAATTGTGAATTTTAATTGCAAGTATAAGTTCGTAACAATTGAAAATTATTATTAATATTGTTAGTGAATTTATCAAATTTAAATAAAAACAATTCTCTTCGTCTCAATTTATATGATATAATGTTACTGAATTCAGAAATTTAAAAAGAAAATATAATATGAGATATCTAAACAGCTCAAATACATCCATACCGATTTGAAAGGAATAAAAACAAAAAAAAGATAATAATCTAGACCAACTAACTTTATTAGGAATAGTTCATTTAGAATAAAATATCAATTACCTAATCAAATTTGTTTCTTTTTGCAAGAAAAAAAGTATGAATCAATTTACATCTTTTTCTTGTTTTTATTCCTTCAAATTCTTACTACTCACTACATAATAAATTATTATATTTTAGGCCATAGACTCATAGTATTAAATAAAATATTTTTGGTACGAATATAACGACCATTAATATTCTAGCTTTAGTTTACTTTGATTTCACAATTATAAATCATATTGTATGCTCCTTCAAACATAATCATGTCTGCTATAAATTTATAAATAAATAATGCAATCACTAACATATTACATACCTCCAATAATTTTACAAATATCTCAAAGTAACAATTAATAGTGAATATGAAAAATAAAATACTAAGTGAATATTAGATAAAATTACTAAAAAAGTAGAAAATGAGAAAAGAAAATTAGCTTCGTTATTTTTAATTTTCAAATCTTTTTTACTGAAAAGCGATGTTATGCTATAACGAATAATAAAAAATAAAAGGAAAAATATATAATATCAAGTGTCACAAAGAGTACACTGTACAACCAAATCAATAAATTTAAATTCATAGCTAAGCAAATATTTTAGTAAAAACTATATATTAAAATTCTCTGTTAGCAAAAATCCAAACTTGTGAGCTACTTCTTTTTTCTTCTTTAATTCAGTTATCCACCATATCGTGTCGTGTAGTATTTTGTAAACAAGAACCAAAAATAGAAATACTCTCCCTCCCTCGTAACTTCGGTTACACTTTTCAAATTTTGAAATTTAAATAAATTTAAATTTAACTATAAAATTTTTATATATTTTTTAGTTATTCTGAATTGTTAATTATATATTGTGACTTATAATATTTTTTACGTAGTTTATAAATATATAGTTTCATTTCAAATTTCTTTTTTGAAGATTTCACGTGCAAACTTAAATGATTTGACTCTTGAAAAACGACAAGTATCTAGACGGAGAGTGTAACCTTTTTCTTTCCCATTTTACCCTCATCTTCTTTACCTTAACCCCACAAAATTGCCCTCTCTTTTTTCAAAAAAATCATCCCTTTACCTTCACTTTTTTTCTTGTTTTCTTGCTTTCTTGCACTGTAAGTGCCACTTCTCTGCATAAAGTTTCCATCTTTTTTGTCTTTAAGAATTTCTGACACCCAATTGTTTCAGCTCTCTGGTCTCCTCTGTCTTTTTTTTTTCAATATATGTTTTGGGGTTCAAGAAAGAGTAACAAGCTCAAAAGGACAAGAAAAGGGGAGGATGAGTACATCAACTAGTATTTTTGTGATAAGATGGATCAATTTTCTTACAATGGTATGAACCCCTTTTCATATTTTCAGTTTCTTTTGAGCTCCAGCTGAGTTTTCCAGCTCTGCTGCCTTATGTGAACTTGGGTTGTTTAGTTTTTGCTTCATTGAGTAGAGAAGATGTATTTGTAAACTGGTTTGGGGTTTGTGTTTCTTGATGTGGTTGGCAACTTTACTTTGATGTTTGTTAGTTTAGTGTTTCAATTTATTCTGAAATGCTATTGCTTTTGGTTCAATGTGATTGGTAGTTGTTCGGTGAGCTTGATTTATTTGTTGTTTTAGGAGAAAAGAACCAATTTTTACCTCCTTTAGTTCTAGTTTTCAATCTGAGGTAGAATATTTTGCTATGTGTTTAGACTTTAGCATGGTGGATAATGTTTTCTAAGAAAATAAGTGAGTTTTTTACTTTTTTTGTGTGTTCGACAAGTAAGCAAAAGATATTGTCCTGAAATGAGAGGTGGGATTTGGATGTGAGATGCGGTGTGTTGGAGGGTGGGGAGAACACGATCAATGTGGAATATGACTAGAAAAACTTGTTTCTCGAAGTCATTTTCGTCATTTTTAAGAAGCTTGTTTTCCTAGAGAATGTGTTTTTCGAAATATTTGACCAATCTAACGTGGGAAAATTGGAAAACATTAGTGAACACATGCTCTATCTTTTTTGTTTTCAAATTGAGGTTTTTCTAGTTCTTAATTTAGAGCAGGAAGTTTATCTTTTTTGGTGGTGTTTAACTGTTTT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 21
GEN 3040
TGTACTCCATTTGGATCTTGAAAATGCAGCTGTTAGCTGTGGGCGTCATAGGTTTTGGGATTTGGATGAGCACTCATCATGATGTCTGTCGAAAGTCTCTCACTCTGCCTGTTCTAGGTCTTGGAGGAGTTATATTCGTCGTGTAGGTTTCTAGAAAATGTTTTCCTTCACATAAAAACGCACTTGTTCATTTAGTTTTCCCTTTAACCCTTAAACCACTCAAGGTTTGTTTATGTATATTGTGATAATCATTCTTCTTTACATGATTTTCAGATCAATTGTTGGGTTTCTTGGAGCATGGAAGAACAACTCCATCTTGTTGTGGATTGTATCCTCACTTTATCAAATGTCACATGAAGTGCAATTTTTTTTCTTATTCTTGATTGACATAACATTTTTTGGTTGCTTCTCCTTTACGTTATATTTCAGTATCTGATCATGTTGTGCCTAATTTTGGTGGCAATATTGGTCTTCACCGTTTTAGCGTAAGTAATGCTTCTTGGAATTTTATTAAGAAATTGGTGCATTATGTCATTTTCTGCGCTAATTATCATGCACACAATCACTAAATGTGTAATTTTTTTTATGGAGGCGATTTCAGTACAAAGTTAAAAGGCATGACCAAATTCGTAACTGTAGTTGTGGTCCACCAAGATATTTTAGTCATTGCTATGTTTATGATCCATTAAAAGTAACTATAGTTTTTTTTTTTGCATCCTGCTTGTTTTATGCTTTACAATTTACAAGCCATAATGGTGTATTATTGAGAGTTCTTTAGGAGTCTATCAATAGCTAATTAGCTACAGCACTGTTCTTCATTGCCTCTGGCTTGGTCCCCTGACTGGAACATACTCTACTTCTTGGAGGCTGGTTTCACCGCCTTGGCTAAGTCACTTTTATTAGGGAAGAATAGAAATACAATATCATGAATATCCTTAACTAGTTGTTCTATAAGTTCCAGCATTGTGTGTCTTGTTCCTGATCCATATTCCTTCTGCGCAGTGGGCTTATATTTTGGATACAACTTCTAATGTACTAAAATGAGCTTTAGAAAGAGTCAAATGGTGTATAGATTTGTTCAAATTTGGTAAGCGCCCTAGCACCGCCAGGTTGAAGCCTATTTCCTTTTTCATTTTAGAGTAAAACACATAATGATTATTTTATAAAGGAGAATTCTTTCTTCAAGTATTTTTTTCTTTGGCCTGTTAACCAGCAATTGGTAGAGGCTTTAGCAGTTAAAGTAGCAACATTAGGTGTAACGTTCATAGGTCTACTTGAATTCTTTTATAACCAAGAGACTGGTTGTTTCTAGCTCCAAAACTTGTTGAGAATTCGCTCCTGTACCCTTCTCCTCTTTAATACCACATTTGGTTCGCAACAGGGTTAGAACTTATTGCTTGTGCTTACCACAAATCCCGCATCGTACTACCTTTGCTATTGAACCAAATCCCTGAGTGAGGACTCCTCTGATTCTACTGGAACATGATACAATTTCCAATGTTAGTGTGGCAAGCCACAATGGCTCAGCTGAGACTTCAGCAGGTGAAGTAGTAATGCTACATGCCGTCTAAAGTGCATAAGACTTTCTTAAGTCCTTCTGGACCATATAGGAAGTTCCAGTGTTACATCAGAACTTATTGATATCAACCCAAGTAATGGTACTGCTCCCCTCTTTAGTATTCGATGGGACTAATTTCGTGTATTGGTCAGTATGTGGATTAAGCCACTAATCTTGTGAGGAGACTGGTGAAGTAGTATCGGCAAAGGAAGAGAGACTTACATATGGTGTTCAGTGACCTTGAAAAGGCGTATGATAAAGCACCTACAGTTTCTTTCCAGCCATACTGCCCGCTTAATGCAAAAAGGAATGCACAACTAATCTTCTTCTGCTTTTCCTTCTTTTTTTTTTCACGCAGTGACAAAAACTACACTAGCAAGTGATAGTCTAAGCATGAACCAGTCCAGCTTGGTTTTCAGTTAACTTCATTACTAGTTCTATCTATAATCTGTCTTGAACGTGGAAATCTAAACTGGTTTTCCATATTCAATCCTTCATTAAACGGCAACTACTGCTGTGTCAGGGTTAACAGGCATAACTCACAAGGTGGCAATTACTGTGTCTATGTGCATGCTGATGTCAGCAATGGCTATTACCTACATATTACTGTGAAACTTATTTTGGTTTGCTTTATCACAGGTTCATTGTGACGAATAACAGCTCAGGTCACAGTGTTAATGGACAAAGGTACAATCACCTGTCATACTGTCTGTGATCACAATATCTCCTTCTCTTCATCCTTCAGTATCTTCCCCTTTATCCACTCTTTTTCTCGACCCACGTCTTGCTCAGTCTCACTTTGGTTCTCAATGTGTCTCAAAGACATGGATTGTGCTTGTATATCTCATCATGACCGTCACAAGTTTTTCCAGTTTTCCTTTTTCCTTTTACTTCTGTGATATTCTTAGAAGAGTGAGGTCACTAATGGTTTTTGTGTGGGCAGGTACAAGGAGTATCAACTTCATGACTACAGTTCGTGGTTTCTAAAACAAGTAAGCACCTCTTGTATGCAAATACCACCTTTGTTACAATTTATGTGACACACTTTCCTTTTTAGCCCGTCCCAAAAAGAGTGTCACATTTCTTTAATTTGAAACAATTTAACTTAAATTTCCATCTTACTCTTAATGAAATTATGTATAGCCACAGGACCACAAAAATTCAAGTCTTCCTTTCTTTCTTAAAATTATGTCAAGTCAATCGGTGCCACATAAATTGGGACGGAGGAAGTAATAAGTCGCAGGTTATAGGGCAAAGGCATCATGTCCTGATCGTTATAGGAGTTGTTTCAGATGCCTTAAGATGAAAATTTCATGCATTTATTAACCATTTGCTCATTTGTTTCAGCTCAATAACACCCATAACTGGAAGCATCTGAAAAGCTGTCTTGTCAAGGCTGATGACTGTAGTAACCTATCAAAACGATACAGGGTCATGTTCCGACTTGAACCTGTTCGTGATTTATATCTGTGAAATCAAATTATTAACGCTTCTGCTGTTTTGTTGCAGACTGCCAAGCAATACAAATTAGCAAAACTAACGCCTGTTGAAGCTGGTTGCTGCAGACCACCATCTGAGTAAGTTATCGTTATTATTACTTGTTTGTTATTCCTTTTCTTTCTTTTGCTAGATATTTTCGTTTCATGCTACTCCGAGTTGTGCAAGTCACATATATCTTTTGTATATGTTGTTTCACCAATTCCTCTATATCTCGCGTGTTTGATATTGAAATCCTTACTAGCATCATCCCATTCATTCCAAGGACTCAGAAATACTCTGCTCCTTTTGTAGATGTGGTTACCCTGCTGTTAACGCCTCATACTATGACTTGAGCTTTCATCCGACGAGTTCAAGCAAGGACTGCAAGCTTTACAAAAACTCGAAGAACATCAAGTGCTATAGCTGTGATTCCTGCAAGTAAGAGTCATCTTCTGGTCTTGTCATTCTTCGCTTAGGTTATGCAGCTCACGGCTCTTCTCCTACTCTATTATCTTACAGGGCTGGTGTTGCACAATACATGAAAACTGAATGGAGAGTGGTTGCTATCTTCAATGTGATTCTCTTTGTCGTTTTGGTAAGTTCACTCTTCTAATTAACATCTCAAAAAGTGAGCATAATGACTCTTGTTTCTTGTTTGAGAAGTACGGTTACATTTATGGGCTAAGATTTGAAGTTTATGTGTTCTGAACTTGCCACCAAATCCATAACTCTTCTGAATTACTGAATTCACAACTACATATTTAATGGATTTCCACAAATATAGGTTCTTAGTTAAAGTTACTGGATTCGTCTAAACTCATAGCTAGTAATCTTGCTCCACTTCTGTGATTTCTCCGATGACTTGAATTGGTTCATATGACTTCTTCTCACTTTATCAGTCGATGATCTACTTCGTTGGATGCTGTGCAAGACGCAATGCTGCTCGTGAACGC
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 22
GEN 3040
TCTAAAATTTGAATTCCGCCACTGCAGAATCCAGATGAGATGAGTTGGTGTTCTTCAGCTGATGATAACAGTTCATGTTTTTCAGCTATTTGTTGACAAACTTTGTAAAGCTTTATTTTAGTGAAAAAAAAATGATTCATGTAATATAAAAGATTTTTTTATGTTTAAGACCATATAATTTTGAAGTGTTTAAAAGTATACAAGCCTGACTCTCTTTGTGTGGAAAGTAAACCAAGATAAAATATTACGATAATTGAAATACAAGCAATAACAGATAGTAACATAAAATAAATGGACAAGGCCTAAAATACCCTTAAAGTATTGAAAATGGTACAAAATTACCCTTCATCCACCTATTGGCTCCAAAATGCTCTTTCCATCCACCCGTTGGCTCCAAAATACCCTTGTCATCCACCTTTGGATTCAAAATTGACCATTTTCAATTGTTTTAAGATTAAATTCTTTAAATATTTTTTTTTATATACTGGGCGTTCAACTATTAATTATAATTTTAATTTATTAATATAATTTATAAACTAATCCACTACCCATTCATTACAAACTAAACCTCACTCAATTAATAATTCAATTATAATATCAAAATTGTCATAAACACTACTAAAACACGATGAAATTATAGATTACTGAAACTGACATTCAAAATTATTCGAGTCCGAATCGAAGCCCCAATGAAATTTAGGTTGAGCCGCTTATTTAGGAGGACACTTTCTTTCAAAATTGAATTAGAAAATTATGATTAAAGGTAAAGAAATAATACATCCCGATTTAATTAATGCCTCTTATTTTAAATATAATTTTATAAATATTTATGATTTATTTTAAAACCTTTAATATATTATTTTGAAAAAAAGTTACCTATGAGGTAACATCACATAATTAAGACGTAAGAATAATTAAGATGAACATAGTCAGACTTCTAAGTTTATCGGTGATTTTTATGTAGCCACTTGAATGTATGATAATTTTCTTTTATATTTTTTAAAAACACTCAATTGGTAGTCGCTTGCATTAATAATTTGACGGGTTCATTAATTTAGAGGGATTTAATTAGTAATGGGTGTGTAGTGGATTGATTTATAAATTACACTAATAAGTTAAATTATAACAAATAGTTGAGCGCCACGTATTTTAAAAAATATTTAAATATAAAACCGTTAAATAAGTGGTCAACTTTGAACCAAAAGGTGGATGACAAGGGTATTTTGGACCTAATAGGTGGGTGGGAAGGGCATTTTGGAGCCAATAGGTGGATGAAGGGTAATTTTGTATCATTTTCAATACTTTAAGGGTATTTTAGGCCCTTTTCCGTAAAATAAAATGACAAGAAAATTAAATAAGTAATAGTACGACTACTAGTATGGACAAATAGAAAGACAACACGCTACTTTCTACTAACTTTCTATTATAATTCGTGTTCTCCACAAACTTCGTTTCTAAGGTCCTGTTCTCAGTAAGCTGGTATTGCATCAAGTTTTGTCTAAGTACGTATCTCAATACTCTTTTGACCTCCTTCTTCTCTTGAAACCAACTATAATAAACCCTTCTCACCTCCGCGATGGAGCATATGTGTATAATTTTTTTTTACATATCTGAATAATCTCAATCTCGCTTTTCATCTTGTCCTGTAATAATATTAATTTCACTGTCCAGTATATTTTCATTCCTAATCTTATTTCTGATAGTATGTCCACACAATTATCCAAACGTCCTCACTTCTATGATCGCTATCGAAGAAAATGATCCTTATAAGAAAATTATCTCTCAAACTTCAATTTTTTATTTCTACATGTTCAAACAATATTCAATCATTTTAACCTCGACAAATACGTAACTCTACCACAATCCACATTGTTTATTAAAAAAAAACATACTTTATTCTTTAACACATATTTATAAATTCAAAATTTTATCTTAATAATATTGATATTATTGTAAAAAATTATGTATTACATGAAGCGGAGAATACTAGTATCATGTAAAATTGTATTACTCCGTAATATATTTTTTTAACTCCCTAATTGGAAATTATTTTCATGAATTCTTAGGCTTGTTTAGTTGGGTTCACGCAAGTGGAGAATGCAATTATTATTAAAAAAAGTAAAAATTTAAGGAATCCCATAGTGTAATTCAATAATTATATTTTGTCCAGACAAAATTAGTATTTACAAAAAATTCCTTAAATTTGTTGTTCCGTGATACTTTAGACTCTAGTGATACATGGTAAATGATACATGGTAAGTTTAAACTGTTGAGAAAAAAATGGGGAAAAAACGGTATAATTAATGTTGTTACTAAAACAGCTTAATTTAATGTAACAGATCATTAATCACCATAAAATCAGCACGTAATTGGATAACATTCTCCCCTATGTATAAGGTAACAGATCATTAACATTTGGATAACATTCTCCCCTATATATTTTTCAAAGTCCTTTAGGAAATTGTTGTTAAAATTGACAGCAAACCTTATGGGATGTGTTGGTATCTTTTTAATCTTGTACTTCAGTGGCTGCAATTTGAAAATAAAACAGCAAATTATAACGATATTGAAATATAGATACTAAATAAAACTAATAACTTACTGATACATGGTTTCAGTTTTCAGAAATTCATACTACATGAAAAACATGTGATACATGTCTACTACATGTATCAGTGAATTAACTAAAACATTATAACATGTGATACATATCTAATGCATGTATCAGAAACATCGACTAAACAATATACACACTGATTCTATATGTATCATCAAGTACAGTGGATGAAACATTTATCAAACTTAGTGAATGATACATTATAACAATTTTCAAAACATGTATCACTACATCAACCAAACAACTAATACCTTACTGATTTATGAAAGTGAATATCATATATATATATATATATATATATATATACACACACACACATCATTTGAACCTTTTGTAAGGTGACCGTTCATTTTCATTTGCTTGCCTCCAAGAACAAGAAGATTACCAAATTCATCTGCTTGCCTCCAAGAACAAGAAGATTACCAAATTCATCTGCTTTTTGGTTGTTCGTTTAATCTTTCCTCTGATTGGAATTTGTTTCTTCGGTAACTGAACTTGGGGGTTTAGGGTTTTTATTTTTGAGAGTTGTTAAGCATGTGGGTTTACGTTGAAGTTGCAATGGCGGAGTAAGTAATCTTTAAGAGAAGGAAGATGATTATGAAAAAAAAAGAACCGAAACTTGATGGAGTAAGAACAAAACGGGAGAAGTTAAGAGAAGTTGTTCTGATTTTTGAAATTTCAAAATTTTTTAATTTTGAAATTCAAAATTTCAAATTTGGGTCTTTTATTTAAAATTAATTAAAATTAATAATTCAAAATCAAAAAACTGATTTAAAAGGTTGAGTGTTGAAATGGAGAAAATTCAAAATCCAAAAACTGATTTAAAAGGTTGAGTGTTTTAATGGAGAAAAAATAGGCATTAAAATGGATAATTGTGTATTATAGGAGAGAGAATGTAATGTATCTATAAGCTTACACTAAAATTAAAAAAAAGGGAATTATGTAATATTTAAAAAAAGAAGAAAAAATTAGAGAATATAAACTTGTAGTTGTGTATTTAAGTTATTTTTCCTTAAAAAAATTGAAATATTTTTGGTATATTATATCATGTCTGAAATCCCAAAAGTTGGGAAAAAATCATGTCTTAAATCCCAAAAGTTGGGAATATTGGCATTTGGACCTCTAACAAAAGTACATAATTAATGTTTTTATTTTACTTTATGCAAGTGCAGAATATTATTTGATTATTATAATTAAAAAATCAAAGATTTTGGTGTAAAACTATGTTTATTATAATTATATTCACACTTACTAGTTACTAATCCTATTTTAGCAAAATCCAAAAAAGTATACAAATTACTTTTAATCTTTTTAAATAAGCAATCCCATAAATTGGTTCTCTCCATTTTATATTAAGTTAATTTTTGAGTTGC
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 23
GEN 3040
TTCATACATTTAAGAAAAGTAAATTAAGACGTAAATTGAATCTAGTTTTCATGTTTTATCTTATTAATCATTGTCAAATTTATAATTAAAATGACTGAATGTTAATTACTGTTAATTTCAATACTAACTAATAAAGGATAAAATTTGAAGAACACTATAAAAATAGTCTTAAAAATTAAACAATTAAGTTAATTTGAAAAATGAAAAATGAATCAAAATTAACTTAATATGGAATGAAGTGAGTAATATTATTACTCGTTCCGTCCCTAATTACTTGTCAAATTTTCCTTTTTTATTTGTTTATTTTGACAAATCAAAAAATTTAAATAATAGAAATAAAATAATAAATTCACTATGTCAAACATTATTTTTCTAACCGGTGAGTCAAATGAAAAAATAAATAAATACATAATTAGGAACAGAGAATACAAAAAAAAAAAAGGTCTAATTTAATCTCAACCGTCAAAAAGCGCGGGAAAATTAATATCCAAAGGCCAACAAAAATGGCCTTATAAATAACACCATTCTCACTTCATTTTTTTCACAAAAATACTGCAAAAAAAAAAACCCTAGAAATCTCTGCAATGGCACCAAAGAGAAAGGCAACTGCAGGGGAGCCATTGCCAACTCGTATAACTCGAAGCCAAACCAAGAATGACAACTCGAAACCCGTTTCAGTTGACCCGGTTTCACCAAAACCAAAACGGGTCAAAAAGGGTACCGTTTCTGAGACGAAATCTAAAGTGAAGGCTCCAGTTGATTCCGGGTCGAAGAAGATTGTTGTTGTTGAGCACTGTACCCAGTGCAGGCAGTTCAAGATTCGGGCTGTGAAGGTGAAGGAGGAGCTGGAACATGGGGTACCCGGTTTGGAGGTTCGGGTCAACCCGGAAAAGCCAAGGAGGGGTTGTTTTGAAATCCGGGTCGATGATGAAAAGGGTGAGAAGTTTGTTAGCCTTTTGGATATGAAGAGGCCATTTGGACCCATGAAGGCACTTAATATGGACAAGGTCATTTCTGAAATTATTGAGAAAATTAAGTGATTCATTTTAATTTTAATTTTATTTTGCTTAATGGGGATTTAGGGTTATGTTTGGATTTTAAGGGTATGATTTTGTGTTTTGTATGACTAAAGTATTTGATATTTTCAGGTCATTTGTAGCCCTTCGAAAAATATGGAAACAATCTGTTGTGAATTTTGCCTTTTGTTTTCCCAAAACTATGGTTAAAATGAAATGATTAGTGTGGTTGAAACTCTATTATATTCGAGATTTGAAATTTATTGATTTTGAATGTCTTTGGATCAATGTCCAATTATGATTGTGTTCACAACCAAAATTTTATGATGTTATTGGATGATTAATTTATTATATCAACAAGGTTTGTGTTAGGTTAATTGGATTTTGTGAACTTTAGTATATAGGAAATGATTTTTTCATTAATGAATAAACTTGTTAGGTTAACCAAGCCATGGTTGCACAAGCATCAATTGTGTGAGCATGGCGTGTTGGCATTGGCGGAGCCACCTTGGTACAAGGGGATCATCCGAACAAACATCTTTCGACGAAAAATTATACTATTTATATATGGTTAAAATAATATTTTATCTATATATTCCTTTGACTACTCCGTGTGTCTTGTTTTTATAAATTTTGAACTCCTTTATTGAAAATCATGATTCCGCCCGGTGTGTGTTGGCATTGGACTTTTGAGAAGTATTTGGAAGATGAGCAATGTGAGAGAGAATATATTCTAAGAGTATCAAATAACTTTAGCAAGTACTTGTGGTGGTGAATTCAAGGTGTGTGCCACTCACAAGTTCTATAGCCATGAGTGTGGTGTGTTGATATTGAGCTTGAGCTTTTGAGAAGTAGCATGGGTGGCACTCATATTTTTCCTGAGTTATAAGTGTAGTATATTGATGTGGGGCATTTAAGAAGTATTGATAAAGGATCAATCTGAGAAGTAGGGTTGTTCATGGTTATGGTTAACGATCAAATCAAACCGCAATTCGAATCAAATCGATATCTGGTTTAGTTTAGTTAGGTTTGATTTTTAAATTTTGGAAACCGATACTATTTGGTTTGGTTTTGGTTTTACTAAAAAACAACTGCAAAAATAACCAAATCGAACTGATAAATTAGATATATAAATTTTATAATTATTTATATATTATTCATAAATAAATTAAATATATTGTTTAATTTTAATTAACTTAAGGCTTTAACTTTACCATTTTTTCAAGTCTAACACTTTAGCCAAGGTATAACAATCTCAAATCCAAGTCCATCAAAATCCGTTTTAGTCTTTACTTATTGTACCTCACATAAAATAGTCACAGTTGTCCTTTTTGCATCTAGGCCTACACCAGTTTATCTTGCGGCCTCATCATATTTGTATTAAATACGTGTATATCATGTGAACTTGAATATATGCCTTATAAGCAATTGGGGATCTCTACGAGGAGCAATTACAACTCTAAGTTCTAATACTTTGCACCACTTCTTTGTTTATATAAAAGTTGGAGATATTGTTACCATTTGACAGAAAGGGCAAGTATAGAAAAATATACGACTCGGAATTTTCATAAGCAACCTGGATTTGATCATTTCCAATTGAAGAATTGTAATTTTCTTTCTCAGCTACATCTTTACATTAGTTTTTATTCCATACAATCTAATTTTTATAAACAAAATTTGAAATAAATATTGTTGTACAAATATTACGCCTCATAAAACTTACCTTTTTAGGTCGCAGATGAAACCTAATATCTCAAATTTACAAAGAGATGACATTCCATTGAACATTAGTAGGCATTATGAGTTTGGAGATGCACTCACAAGAAAGAAGCTAGTTGTGGGCTTCGATAGCCTACTAAAATGATACGAGTGATACACAGTAAATCTCAAAAATTGACTCGTGGATAATGGTGATGGTTACGTGAAACTTAGTTCAAAGGGGGTGATTGTCAGGTTTGCAAACAAAATTTCATTGTTCTACATGAATAGCTAAAAAGATTGAATAACTCATATAAAGTGCATAATCTCTTATTAGTGTGAGGTTGACTGGGGAAAAACCATTTGAGTTTGGCCCAAAGTAAACAATATAACACCATGTAAAAGTATCTTTGGACTGGCTAAGCGCAACGTATAATTTAACTTTTTTTTACGTTGAAAATACAATAGTAAGATAAATAAATGATATAATGTAGGAAGAGTATTTCAAAGATTACAAATAATATCAGCAATAAAATCGTAAAATAGTTTATAATTAAAAAATGTTTATTACGTGTAAAATCTTAAGTTTAGATAATCTTATGACTTTTGACTTATAACATTTTAATATTTATCAAAATAGTTTATAAAATTAGTCAAAACACATTTATAAGAAATGACTTACTTACTAGTTTACCTTATCTGCATCATCAAAAAGATGTCCAAAAAAATTATTTCAGATTCTCCTAAATTTATTCCCTTTTTAAAGAAAGCCAAAATGTAGAAAGTTAATGGTAACATTACTATTTTTAATTGCACCATTTGAATAAATACTTCCCCATCTTCTACTAATATACTAGTTTAATTTCAGATTATTTGCATTTTGTTACAAAAATCATCTCCAAAGTCATGTCATATCAGGTAACTTTTCAAAATTCATTTTCGTTAGGTTTGAATTTTTTACTCGTATCTATTGATGATGGGAGCTGACAGATATTCTATAGAATTAGTTAAGTTCTGTAAAAAGTTAGATCCGAATAAACAAAAAGATTGCTTTGAATTTTTGTTTGCTAGTATAAATAATGTTGTTTATGACTTTTGAATTTTCTTGTGTGTTTCCATGAAATTGAGGTGATCATTTATATATATTTCTATTCCAATTCTTTGTTTGGGTCATTTATATATATTTCTAATCCAAAAGTTTGCTTAATTATTTCTCAAATTTATGTAGAAATCATTGATGCATCTGAGAGCTAAAGTACAAATTGTGGTTGCAATT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 24
GEN 3040
TCACTATTGTTGAGCTATGCCAAAATTGAAGCAAGACAAAGCCTATCTATGGTTGAAGATTTGGAATTGGAGAGGCAATTGAAGCTTATAAATAAGCCAGCTATCAAAACGATTAAGGTACAGTCTCGATTTTTTATATAAAGATTAAAAGAAACATCTTTTGTCTTACGTGATGGAAAAGTAATAATGATCACAGTATAAATATTAAATCTAATAGTTTGTACTACATCTATTTTTTTTCTATTAAAAAAAATTGATTGGAGACTAATTACTAGTACTATTTGATAAGCAGGGGAAATATGGAGAATATATGATTGTGTAGACTTTTACAAACAACCTGCATTCGACAATCCTCTATTGAAGAATCACGAATTTCATCCTGAGGTATATCTCTACCTTAGTTTCAAGTTCGTACAATCAAGTTTTTATAATCAAAATTTGAACCAAATTTAAGACACAATTTCTTTACCTCTTAAGATAAGAGTAAGGTCTGTGTACACTCTACCTTCTACAGATTCTACTTGAGAAAATTCACTGAAAATATGATGTTGGATACAGATGAAACCTACCTTGCCCAATTTACAAGGAGATGACACTTCATCAAACATTAATAGCCCTTTTACCATAGGATTAAAAGACGGAGGTTGTCCTACTGGAACAGTTCCTATCAGAAGAATTACTAAAGACGATCTAATACGACAAAGATTCTTATCACAAATTAGAGGCGCGGATGAATCCCCTTATGGTGATGTGAGCTTGCTGAATTTCTACAACTTCTTTTCGAATTTCATAATTCAAATTCTTTTATCATATTTTTCTTCAGGATATGGAGTTCAGTGTTTTTGATGGAACCAACTCAAGTATACCTTTTAAAGGAGGACGCAAGGTTTATATACTTTTATTATTCCATATTCTATCCACAAAAAACTAATTTTTTCGATATTTTAACACATTATATCCACCCACCTCCCAAAAATAGCCAACATATGTATAATATATGTATATACATGTATAGCAATGTATAATTAGCTTAAAAAATGTTTTGTTTTTGCTTTGTTTCAGTATGCAACAGTGCAAATACCATACAACTCAACAAACAAAATAACAGGAGCTGGGGCAATTATTAGCTTACATAATCCTCAAAATCTTAGTGGACATCAATTTAGTGCTGGTCGTATTAAGGTTCAAATTGGAATCGAAAGTATACAAGTCGGTTGGATAGTAAGTTTTTTGTCTACGACTCGAGTAATTATATGTGTGATTGCATCTACTGACTCTAAATCCTGCATCAGACTAAATATGCTAATCGAAGTTACCTAATAAATTTATATTAGGTGAATCCACATGTCTATGGAGACACTCATACGCGATTATACATATATTTAAAGGTAGTTTTCTTTAACTTCTTAAGAATTAAAACTAATTAAGATATAATTTTCTTGAAAGTCGATGTATTTATTAATAAATTTTTTTATGATCGCAGACAGAAAAATTAGCATGTTTTAATACACGATGTCCTGGATTTATTCATATAAATACTGCAATACCTTTAGATGGAGATCTTCCTGCCTCTACTTATGGTGGACCTATTTATGATGTACCAATGTACATTGCTCGTGTAAGTGTGCATCTTCCCCTTTTTTTTATAAGTTTTTCACTCGATATCTGATATTTGTTTAAGGATCTTAATTAATTGAATTTACGTTTGAGTAGAATTTATTAAAAAGAAACTTTTTAAACTATTGCATATAGATTATTTTTTTGAGCGAGTGGTAAATTTTATGAGAAAACGTTAGACTAGTGTCGAAGGTTTGTAGACATACACTACACAACAATATAATGTTTTGTGAATTCCAAGAGCTATATTTGCACAACTTTCAAAGACAAACAAAATCTAAAATTGTGGCATGGAAAGAACATTCGTGTAAATCTTCCTTCTTCTTCTGTTGTTCATATTCTGAGTTCGAATTTAAAATACTTTAATTAAAGGTGAAATAATTTTTTTCATCAGAAAACGTTTTATAATCCAATTATGATAATTTTTTTAAAAAATAATGAATATGTATATTCATGTAACATGTTATAACATGTTACTTATCATTTATTCTATATTACTAGTGAATGTTTATTATATACAATAACATGTAATATCTTGTAAGTGATTTGATTAGATAAATAATACACACTATCAATCCATAAACTTAACCTTCTTTTATTATTATTATACAGTATCTGACTTTTTTTTTCCTTTTACCATTTATAGGATATGTCAAATGGAAATTGGTGGTTTAAATTTGGAACAAACTATACATCAGTTGGATTTTGGCCATCAAAGATATTTACAAAGTTAAATGAATTTGCAACAAGTGTAGAATATGGAGGAATCGTATATAGTCCACCAGGTGTACCAGAACCTTCTATGGGCGGTGGCTATTTTCCAGTTGGAGATTTGAACAAAGATGGATATTGTAAGAATAGTACCTATTTAACAGATAAAAATGAAACTAAAAAATCTTTAGATGATATTGAAGTGAAATTATATGCAAATAGTCCAAATTTGTATAGAGTTGCTGATTTTCCAAACTCTGGGGTTGAATCTGGTAATTTAGTCCTATATGGAGGACCAGGTGAACATATGTATAAGAAACATAGAGATATTTGATATATAGATGCGGTGTTTTAAGATATTTCGATATTATATTTATGAATTATATATGGTTTGTTCATGCAGTGGTTCTTGGTATATAAGTAAGTAGAATGTGTCAACTAGTGGTGCATTTTAAAGCCGTTCAAGTCGAGGTTTAGAACGGACAATATCACTAGGTGGCTGGACTGTTACAAAATCATTAGATTTTGTTCAAATCTCGTATTTATATTTTTAAAAATCAACTTAATTCTATAAATATTTAATTATATGTCCAATTCTTTTGTCTGATACGAACTTACATGATCATATTAGAAACTCATAAACTTTAAATTTTGTATCGAACATTAATGAAACTTTTAACTTTTTTCTTCTTCTTTTTGGTTATAATATAGGACATGTCTAATGGAAATTGGTGGTTTAAAAAGGGACCAAACTATACATTAGTTGGAATTTGTCCATCAAAGATACATTAGTTTGATAGTTTAAAATAAAATTTTAGATATTCAAAAGCTATACAGAAAGTATTATGAATTATAATTTTTTTTATATAAATATAATATATATATATATATATATATATATATATTAGTCAATTATTTGACTTAAAGAAGATCCTTTTATAAGAAATCTAAAATAATAATATAGAAAATTAATTGTAACATTTGACTTTTACACTATTCAAATAAATACTCTCTCGTCTTCTACTTAGAGTCTGTTTGACTCAACTTAAAAGCTGGTCAAACTGACTTAAAAGTTGGTTTTTGACTTATTTAACTGTTTGGCAATACTCAAAATAACTTATTTTAAGTTTAAAAAAAACTTATTTTAAGCTAAAAGTTAAAAGCTGGGGTAGAGGTGCTTTTTTTTTAAGCTTATAAGCTGTTTTAAGTTGACCACATTTTTATCTTTTTGCCCTTAATATTTTTATACAATCTCCAAATTACCCATATAACCCTAACATCTCTTTCTTCCATTTTTCCCTTTTCACGTTTGGCATAACAACTTCACCACTATTATCCAAACACATAACTGCTTATTTTAAAAATAAGTTTCAACACTTTCAAAAGTACTTTTTTAAAGCTGCTTTTATTAAGCCCATCCAAACTGGCCTTTAGTATAAATGTATAAGTCAATTATTTGACTTTAAGAAAATCCTTTTATAAGAAATCTAAAATAATAATATGGAAAATTAATTGTAACATTTGACTTTTACACTATTCAAATAAATACTCTCTCATCTTCTACTTAGTATAAATGTATAAGTCAATTATTTGACTTTAAGAAAATCCTTTTATAAGAAATCTAAAATAATAATATAGAAAATTAATTGTAACATTTGACTT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 25
GEN 3040
TTACACTATTCAAATAAATACTCTCTCATCTTCTACTTAGTATAAATGTATATATGTTTGTTTAAGTTCACATGTATTTTTATTTTGCATCAAATTTCAACACCATCGTCATGTCAAATCAGGTAAGTTTTCAGATATTTATTTTCTTAAGCTTTTTGAAATTTCATATCGCTTAATTTTATCATACGTTATATTTTTGTTCATTCATATCTTTGATAACAGTAAATCGTTTCGTGTTTAACCTTTTGTCATTGAGGAATGCTTTACCATGAAATGGATCTACATGTGCCCAATTTTTTCGGGAATGAAAATGTAAATTTACCCTTTACCTTTAGACTGTAGTATAAAATTATTCTCTAGTTGAAATTTCGACAGAGGTTAAGGGTAAACAAGACTTTTTCCCTAACAACAGAGGATTTATATTATATAAATTAGACCAAAAATTTAACACAATGTTGATAAATTACATCTCAAATTGTAGATATTACAAATTGTTGTTGCAATTTCACTATTGTTGAGCTATGCCAAAATTGAAGCAAGACAAAGCCTATCTGAGGTTGAAGATTTAGAATTGGAGAGGCAATTAAAGCTTATAAATAAGCCAGCTATCAAGACTATAAAGGTGATATATATCATAATATTTGAAGCATATCAAAATTATTTATCAAAACGTAGGAAAAAATAAATAAATTCACTTTTTTTGGTTGATTTGTAGACCAATGATGGAGATATATACGATTGCATAGATTTTTATAAGCAACCTACTTTTGATCATCCACTATTACAGAATCATGATTTTCATTCCGAGGTGTGTTTGTATTATATACTTCTATATTCAATTTTAATTAAATTTTATGACAAACATTGATGTATTTTAAATTTGACGTAGATGAAACCTACTCTATCCAAATCAAGAATAGACGCAAAGGCTTTGAGTTTCGAAAAACCTTCAAAGATAGGGTTGAAAGGCGGTGGATGTCCTAGTGGAACAGTTCCTATTAAAAGAGTTACAAAAGAGGATCTCATTCGACATCAACATATGCAAGACATGAAAAAAAATCTTAATGATCATGTAAGTTCACATATTATATTTATAATGCCATATCGATTTTTTATCGAATTCATCATATAAATATGTAATACTTTATATCGCCGATATATAAAATATGATTTATTCTTTAATATAATACCCCACCCCACCCCCCTCAATTTTTTCCTAGTTTTGAATCTCTAACATAAGGTGTACTTGTACTTCATGTAGGGGAACAACATAACCAATTCAAAGGCCTCAATTTCCCTAGATGAAATACATCCTTTCCAGGTGTGTATTGAACCCCACCACCCCCCACCCCCCCACATCCTTTTTTTTTTCAAAATAGATTCGGACACTTTTGAACTTTATTCAATGCATATCTAAACTTGATGTTTGCCTAATTAAACCCGAACATGACAATTTGATAACCTCGTCAAGTTCAGGATAATTAGACCAAAGTAAAGTTCAGATGTACATCGAATAAAAATCGTGAAAAGTGTCCACTTTGAATCATTTGCATATAAATTATCTTACTTTGGGATTATTATTTTATTATTGATTTTAGTATGCAGAAACAGAAATTCCAGGTGCTGATATAAATGTACTTCGTGGAGCTGGCATGATTACTACTATACATGCACCAATGAATGTTCAAAAAGATCAGTTTAGTGGAGCTCGTGTGAGGCTTGAAAATGGACTTAGTGATGCCATTGAAGTTGGTTGGATCGTAAGCTCACTTCTACTTCTCATTTAAAAAAAAATTTAACTTTTATGGACTAACAGTATTCGAAAAGAATACATATATCATCGACGATTATCTGTTGTTACAAGTCAAACTTATAAACTTAATCAGTTATACACGCACGCTTATATATATCATTTTATGCAGTGACAGTGTTTAAAAATAAATACATATATCTTCGACGGATATTTTTTGTAATAACTCATCTTATAAACCTGATGGTGTACTAGAAATTTGTATATATCATCAACTGTTATACATATTATCATTTCATACACTGACAGTCTTTAAAAAGAATACATATATCATCAACAGTTGTATGTTGTTACAAGTTTTCTTATGGTGTACTAGAAATTTGTATATCATAGATTGTTACACATATATCATTTTATGCATTGACAATGTTCGAAAAGAATACATTTATCATTGACGATTATCTGTTGTTTCAACTCATTATTATAAACCTAATGGTGTAGAAATTTGTATTTCATCTACAGTTATACATATATCACTTCATATACTGAGAGGTTTGAAAAGAGTATATATATCATCGACAGTTATACGTTGTTACAAATTATCTTATAAACCTAATATGGTTTAGAAATATCGAGAAAATTACTAAATATCTTTTTTTCTAAGTTGAAAAAAAATACTTGTTATTAATATTATTGTTATGCTAAACTTAATGAAGGTGCATCCTACTCTTAATGGAGACAATACGCCAAGACTTTATGCAAGATTTGAGGTAATTATGAACAAAACTTAATTACTTTGATCAATTATTTAAAACTTTTCTTAGAGACTATAGTGTTTACTAATTATAATGAAATGCTTTTTTTTTAATGTATTATTATAGGTTGGAAGTGCTGGTTGTTTTAATACATTATGTAGTGGCTTTGTCCTAGTAAACACAGATATACCTTTAGGAATGCCACTTGTACCTTCAAGAATTGGTGGACCTATTAATTCACAAATAATGTATCTTGAACAGGTAATTCATATTTATTTGTTCTGTTACGTTAGTGAAATACGTTTTAAAATTTCTACAAAACTTGACGTTGATAAACGCTATTTACGAAATGTTCCTTTTATGTATCTTACTATATGTCAATATAAACTTATATAGGATGTAGCAAATGGAAATTGGTGGGTTATGCTAGGAGAGGATTACAAACAAGTGGGATTTTGGCCTAAAAGTATTTTTACAACATTACAAGCTTATGCAACAGGTGCTAAATATGGAGGTATAACATATAGTGCACAGGGATTACAATTCCCTCCAATGGGTAGTGGACTTTTTCCAAAGAAAAATTTGTTAGAAAATGCATATTTTAGAAAGTGCACTTTTTTGTGTTATGTGAATGATGAAATGGTAACATACTCTTTAGATCATATTGGCACATATCCATTTCAGAGTAACACAACTATATATACAGTTCAAGATTTTATTGAGCAAGGTGATGTACTTGGTCATTTGATTGTTTATGGAGGACCTGGTGGATAAACATTGATGTAATGTTGACATTATGAATTAAAAAATATTTGGACTTTTATCTGTCATATAATTTGTATTTTGCATTCATATTAAGAAAATAATGATTATTTTATGATATTAGGCAGGGGCGGCTCAACGTTATTGGAGGCCTAAAGCAAAATTTCAAGTCGCGACCTAAAATATAAATTTTTTTGAAAAATAACTTGCACTATTTAGATGAAAATTATTACTACTTAATGTTGTTTTTTCAGTTATTAGTTTTAGGTAAGATTTTATCAACTCAACATTGAAAAACATCCTTTAAGTGAGACAATTATTATATGTATTGTTAACATAATGATACAAGTAATAAATTTATAAATATTAAATAATGATTAGAATTAGAATCATAAATTTAACTCAAAAAAGTTGATGATTTGGTATACCTCATTTTAATATGCTTAGAGTAATGCTTGAAACCAAATTTTAAAATAAATAAAGAAGAATTTATAATTCACTTTGTTCAACATTTTTCATTGTTAATTCTTATTTTTAAGAAAGTACAAAAGATGTTAAAGTGGAAAAAATAATTTATTTTTAACAAAATTATACTTTTTATCATAAATAATTTATTTATCTATAAAAATTTTAATACATAATTTATTCTTGACAAAAATTTGAGGCCCCCGAAATTTGAGAGTCTAAGGCAAAGGCTTTATTTTTGAAAGCATAGAGTCGGCC
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 26
GEN 3040
CTAATATTAGGGGTTCATTCGAACTTCCTTCGAGGAAAAATCATATAACATATACATGGTTAAAATCACTTTTTAGGTATATATAAAAGATGTTGAACCCCCTTCGACTACTTTATGTGTCTATTTTTTCTGATTTTGAACCCCTTGTCGAAAATTTTGACTCTGCTACGATATAGTATTGTCTATTAATTGATGTATAGTTTAATGTTAAGGGTAAAAAGTGTAGAAAAAAAATATCTTTTTTTTTAATATGTTAAAAATCACAATAACTAAAATTGAAAGAAAAAAAATTGAATAGTGAATAAGTAAAAATGAATGAAAGGATCGAATATATTGAAGTTTTTTGTATTACTTTCCATACTTTTAAAAAAAAATTAAAAAAATCTCCACACATTGAAGTTTTAAGGGAACATTATAGGATATTTTCTTGATATTAATTGGGTAATAGTTCTTGTGTGAATTTTAAGTGTTAAATGTTGTTGCTCAGTGAAGTGTAGGGAGTAGTCGTCTTGTTATTGATATGTATGAACATGTCAGAATTCGATAAATAAATAGAAAATAAATAATTTTTAAAATTGCACCGTAAAAAAATCAAAACAATTAGTGCCATCAAGACTTTTTGAATTATTAGAAGTTTATTATTTCAATGAATATAAGTTTCATGGGAACTTTGGAGGAGATTTTCTTGACCTTAATTTTGTGGAATAATTAAAAGTTTATTATTATATTTTCAATGAATTTATGTGCCCTCTAGAAACCATATCATTTAATAAGTTGATTATCGACTAGGTCCAAAGAGGCCTACGTCATGTTAGAAACAAAAGTCCTCTTGAATTAATTATTTAATAATTAAATGTTTTGAAAATTATGAGAAAAAATATTTAGTCTAATCATTTTGAAATAGAAAACGTATAATATGATTAATTTGAATTTAAATTTATTAGAACATCATCTATTAGTAATTTTATTTATTTTTCAATTGTATATAAAATAAAAATTAAAAAATCTTATCTATTCAATTATACTATTGATAATACTTAGAAAATTGACCCAAAAGAAAACGACACTATGATCGATCTTATCATGAAGATTTCTTATTTTCTTAAATACGCAAAATATCATGATAAGACATTCATAAATCCAATATAATTTTATTCTTTAAATTCAAAAAAAAAAAGTTCATAATTTCAAATAAAGAATTTAATTGGTATATGTTACCGCATTTCATATTCTTTATTTCCCTAAACATTTATATTTAAGATCTCATAATTTTTAATTTGCATAGTTAATTACTATAAGCTACACTGTTTTGATTCTTAATCATAATTAACCACAACTTTATAATGCATATTGTATTCTTTTTAATTATTTGATAACTATTTAAATTTATGAAACTTTTATACTTTTTTAACGTGGAACGTGAAACACTAAAAGATAATAGAGATAAAAAGAAAAAAAATGTTTGATTGTTCATAATATTATTTTATTTCTTCATTTACTTGTTTTAATTTTTTTTTTCATATTTAAATTTAACATCAAATTACTTTTAAAAAACGTATAAAATAGACAATTAAAAATAAACAAACAAAAGAACGGCAAGAACATGAGACAGTTGCTGGGTTTGCACTTGCACCACGTGCCTATAAGGATGGGCTCCTGAAAAAACTCTCGTTAAAGTCCAACCAAACAATTAATATTAACACGACGATAAAATTTATTCGTATCAGGTGTTTAATTATTTATATAAATATAAATATAAATGTTAAAAAAGATATTGGGTTGCTCCTCTTTTAGTTAGAGGTTTAAGATTTCAGCTTTGCATGAAAAAATTTTAATTTTTTTTTAATAAAACTATATATAACTTAAAATTTAAAATAATCAAACTCTAATACGAATATGGAACCGAAAGAGTTTGTTTTTACCAAATAAATGTCTACACAAACCCACCAAATTCTTGTCAGCTCTTCATAACCTCCAAGCAACATCTTTTTTATTCACACTGCTATTCCCATCTTTCATAAATGACTATTTTACCCTTTTTAATCTTTTCTACCAATACTAAAATTAATATATAAATTTTTTATGGACGTTTCTATTAGAAATATGAAATTTTCAATGAAAATAATTGTCAAAAACGTTTTTGATAAGTATTAAAAAATTTTATTTCATTATTATTCTAAAAATCAACTATATCTTGTTTAAAGTTTCAATTTTTCTCATATGTTCTAAATATGTGAGATGTACCTTTTAGTTGATCTGTACAACGTGAAATCTAATTAGTCGAGTAAGTTGACTTCGAATATCATATGGTAAAACAAAAAGAAACTTCCCATAAACTACTTGAGAATCATAAAAAAAAAGGAAATATTATGAGGGTATATATGTCATTTTAAACTAGACAATATTAGTTTTTTCTGGTTCTTATCTGCACCACAAGTTCTTTAAATTAAGACACATATTAATATAAAGACTTATCACCATTCAACAAAAGTTCTAAAAAAAAACTCAAAATTAGACCAACCTAATTATTTCCCTCTCTGTCCATATTTTCCTAATATTTCTTCTTCATATAATCATCATGAAAATTCAATATCCTTCTTTTAAATTTCTGCTAATATTAGTAATTATACTCTTTGTTAGTCAATTATCAAATTGTAGAAAAATTCAAGAAATCACTCCAACAAGCATAGAACAAAGATTGAATTCAAGAATTTCATGGCATAACCTAGCACCATCTCCAAAAAAATCAAGAAATGAAGATATAGACAAGAGTTATAGAGTATCTCATCGAATCGTTCCCGGAGGACCAAATCCTCTTCACAATTGATCAACTTACGTTCATCTCCAACATTGATAGCTCTGTTCATCGAGACTTAGCATGTAAGAAAGAAAATTATTAAACTTTTTTTCATCTTTATTGGGATTTGAGTGCTAGCCTTTTAAGGTGTTTATCGACTTTGTTGATTGTTAGGTCACAATCTTAGGTGCCCTTTTGTGATCAATTGATGTTGACTTCTTCACATTTCACCAAATTCCCAAGTTCTTTTTGTAGCTAAGTTTGATATTATACAATTTTTTTCCATAATCTTTGTATTGTACATGGAAATCAAAGACGTATACTAGGAAAATCATGATATATTACTTTAAATTGATTCAATTACATTTTATCTTCTCCACTTTCTTTCTCTTGTTTTTTAAGTTTTTTAATTGCATACAAAACGTCAAAATCAACATAACTTATAAAGTTATTGTCATGTGATCAGAAGGTTTCACGTTTGAGCGTTGAAAACAATTTCTTATAAATTGTAAATGATTCTTATAATTTGACTTCACAAATAGCGAGAACTAAACAATTCTTATAAATTGTAAATGATTCTTATAATTTGACTTCACAAATAGCGAGAACTTAACATATTGACCGATTTACGAATAGTAGTAGTTGAAGAGCTACATCATTGCTCATTAAATTTCAAATATTTAAATTATGCAAACAAAAGTATAGTGAGATAAAAGTGTGAAAACATACCAAAAATTAAACCAGTTGTTGAATAGAACTACTAACCAATCAATACTTAGATCAGCCACCGACTTATTACCAAGTGTTTATTAAAAAAATATTTTTTTTTAAAATGTGAAATATATTTTTTTAGAACAAAAAATTGCAAGTGATGTAAGAAAAAGTCAACTCATAGACAATTAAGTAGGTAGGCCGTGGACAAATTAAGTAGCAAATTATTGTTTATTAGTCTCTATTCAAAGCATAGTGCTTAATTTAATAATGTTTAAGTAGTACAAGCAATTTTATATTGGTGGGCAAAACCAGCTAGTTTTCTCATGGATATAAAGACTAAAGAGTACTTATAAAATAATATAATCCACATTATATTAAATTATAGATATTTAATTTTTATAAAGAAGAGAAGGAAAAAAAAACGTTAAGAGTTAAAGATCGTAAACAAATATTGCGTAATTTAATATTTTGGTGTCCTA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 27
GEN 3040
GACATAAGAAAACGATATCGATCCATATTTTAAGAATCAATAAAATAAGTAAAATCATGAAAATTAAAGTTTAAATTTGAATTGTAAAAAAAATGTATGTACGAAATTGCTGAGGACATATAAATTGACTGAATGCGATGATTATAAAATATTAAAAAGATTATATAGATATTAAATATAATAGTTGAGTTGTACACAAATTAATTAGGATATTATTATTATTATTAAAGAAAAAAAGAACAGTATTAGTGCATGGTTACTTGATGAGTTGTTAATCCCCCTCCCTATAGGCTATATATATATAATTTATTATTTTGGCATAGAAAAAAACAACTTTTTTTTTGGAAGTATTTGGTGTCGACTTCAATAATATGGACAATTAATTAATTGTGATGTGAAGTGATTAGTAGTTTAATATGTATTTTGATAAGTAACTTCAAAGAGTACTTAGTCTTCCATGAGAAACACACAAATTAAATAATACAATATCATTATCATGCAAGCGTCTACAAAATGGACTTCTTCGACCCTATCAAATTAAACTAATTCATTATAACAAGTTATTATTTTTATCGAGCCTTAGGGTGTGTTCTGAAAAATATTTTTTTTCAAAAAACGTTTTCTAATCTTTTCATATATCCTGTTGGTCAATATATATATATATATATATATATATATATATATATATAACACAAATTTCTTGAAAGCTAGAAAACATATAAGAAGTAATTAAATAAATTAACTAACCAAATTTCATCGGTGGCATTTCAAATTCATTGTCTCATTTTCATCCATCCGATGCTTCCAACCCCTCAACCCTTGACGACCATTCCACCTCTCGAATTTCGTTATTCTAAGTTATAAATGCTTTTATAAAATAATATAAAAAGTCACTCACTTATCAAATACTAGCAAATAAATAATTATAAAAAACTCATTTATGTTTTTTAAGAAAATGTGGAAAAGCATTTTCTACCACCTTTAGTCATAATATAATTTGGCATGGAAGAATTATAGAATATGAGACTAATGTATCTCAATTTGCTTTCCCACAAAATGTTCAAGCTAAGCAAGAAACTGAAGTTTAAAGTATATTATATAATTAATTAAAGTTCCAATATTATTATTGACTTTATTCACAATACATATTTCTTCAAAGATAGACCTAACACGTAAGCTACTAAAAGTCAAGTCATGTTTGCTGACTAGATAAAATAGAAGTTAAGGTTTGAGAGATTTTTTGTTCGAGCCTTCGAGCAGTTATCTAGACATCATTTTATCCTTAAATATTAAATTTTTTTATGCAAATTTAAGTTTAATCAAACTCTAATGTAGGTACTACGTACTAAATATTAATAAAAAATCAAACTAAAAGAGTTCAAGTTGCTGAAAAAAATGAAATTGAAGATTAGATTTGATTTTTCTTATATCAAATCAATATTTTATTTTAATAATCAAAAATTGAATTGAGAATATATATCAGGTATAATATAACTTTCGTAAGCTAAGTCATGATAAACATATGTATGAAAGACGTATTTTGCATTCATATGATATATGTAAACAAGTTTAATTGGTACAGTAATCTAAATCTCACTTCGTGAATTTCATTTATATGTTGTTGTAATAGACAAACTGATTGTTGAAAAAAGATAAAGACAATAAGAAATAAAAATATATAGAGAAAGTATTAACAAGTAGCACCAGTGGTCTAGTGGTAGAATAGTACCTGCCACGGCACAGACCCGGGTTCGATTCCCGGCTGGTGCATTTCAGAGCAATGATGTTTACTTGCACAAATTGCGAACGATTGGGTCGTCACCTACATCTGATTTATAAGGCAGATAATGTGCATCTGAGCTCTATTTTTTTTATTCGTTTTCGTTTTTCACCTGAATTTAATATATATTGGAGATTGATTATATTTAGAAGCGCTCCGTTTTTCTTTTCCATCCCAGCCTGAGTTGCTTCGTTTTTATTTCCACCCCACTGAGTTGCTTCCTCATCTGTGGTCATATATTATATTTGATTGTTTGTAGAAAAAAAAAAGAGTTTAAATTTTATGTGTTTTAAAAAAACTAATAAGAAAAGTGAATAATGTTTTGAACTCCAATTTATTTAATTCAACCACAACTCCAAAGTTAATGTATACTGAAATCTTCTTTAAAAAGTCAACTTTTTTTGTTGTTGTTATGATAAAATGAGGCAAAAATATAAATGAATTTAGAATTTTATGGCACAATAAACCTACAACTATAATATATATATATACATTATAGTAATATGAAAAATCAGATTCTACAAAAAGAATTTCTTCTTCTAACAGTGTCATTCGACAGTTTATATGCACTTCGATTATACATTATACACACTTCGACTATATATGCATGTAAATATTTTGTTTTTTGCTAATTGATGTCCTTTACTGGTCCCTATAAGACAACTAGTGTTACGATACCCCACTGATGCACAAGAGCAGTGATGTTTACAAATTGTGAACGATTGGGTCGTCACTTACATTTGATTTATAAGATGAGATAATGTGTATCTGAGCTTTACTTTTTTGCTCCTTTTTATCTTGGTGAAAATTTATTTTAATATATATATAATTTTTGGATAAAAGTATTGAATTTCCGTAAATCTGTAACTTTCACGTGTTTTCTTTTACCTAACAATAATAACCATCTCAGTACTAAAACGATAGTGAAGTGAAGTTGTTCTATCATAAAATTAAGAATCGATAAAAGAGTATATTGTGTTATTCTCAAATTTGAAAATAACAATGTACGTTATTATCCATTTTTCAGTTTTTATCACAACCTTTCAAATTATGTTGTTCAAACTTTTCAAAATATGTCAGACAAGTACATATTGAATTCTTCAAAAATATCATATTTTTAAAGAATTAACACGAATACAACAATAGCTTTCATTGTTAAATTTGACTATTTAAACAATATAATTTTTAAGTACATCATAATAATCATTACGTATAAAATAACTTACTCAAAATCAACATGAGAGTGAAGGCTTGGTAGATTATCAACTATAAACTAAGGGCCCGTTTGGCCATAAATTTTCCAAATAATATTTGGGAAAAATTTGGCAAATAATGTTTGTCCATACAATTTGTCATTATTTGGCAAATATTTTTGGTAAATATTCCAAATTTTCAAATACTAGTTTTTTTTAGTATTTGGGCCAAATATTATTATTTGGGATATTTTAAAAATTAAAATTTTACCCCAATCTTTTATCTTTTACAAAAACACCCTCTCTAGTATTTGTTTGCGTTGTATTACATCATTTTTTACGTGAACACCAAAATAGTGATGAAATATTTAGTGAATATTAAATAATGATATGATTGTTGATGAAAATTATTAAAAATTGGCTTTAAGTAACAAAATCATGTACTTTATCTACTTCACGATGTATGGAATAATTCTTGTTATACTCACTCCAAATTATTACATTGCTTCAGTGTCATGGACATTATTTGTTATTGTTGTAACTAACATTCAATTGACTTGTAATACAAACTTTTAGTTAGTTTTGATAGTTTTTAAAACTTGTGTGTATAAATCATATTTTTCTAAAAAGGTGAAATATATTTCCCAAATTTTATGGCCAAACACATGGTGAAATTTCACCCAAATTTTCACTCAAATAATATTTGCCAAAAATATTTGGAAATCTATGACCAAACGCTAGCTAAATATGTCCACTCAAAGATCAGCAAAAAAAGAGTATATAATAAGTTTTAAATTGAATATTTGTGATTAAAGACACTTTTATAAGGTGTTAGCATCCTCATTGTAAGAAAAATTTTGCGCGAATAATTCATTTTTTACTGAAATAATTTTATCACTAAGAAGATTTTTAATCGTCTTTATGATTTACGATACTTATTCCTTATACAATTATTTGAGAAATGATTCTTTCCCTCCGTGATCGATATTAATTTTGCTTTTGTTATAGATGTAACAG
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 28
GEN 3040
TCTATAAACTTTTTAAAATTTTTATCAATTCTATTTTATTTATTTGGCAAGAAGCAATAATTATTCTTGATATGGTTTAATTTCAAAGAGGAAAATTGATAAATTGAATGTGAATCTTCTATGTTTAACTTTAGGATCATCTTATTTATTGAATGTTAGTGGATTTTTTCAATTTTAAATAACAAAGTTGATGGATAATATTTTAAATAGAAAAATTATATTTAAATAACTTGATTTTTATATAGTTAATACGATATAGTATTTATTGTACCGTCTATTATACATACAATAATATATGAATTCATATATACCTTAATATAAATATTTTTAAAATATCGCGCACCTAATACTATATTTATTTATTTCCTTCTATGGCCATTAAATGATGACATTATATTATGCACGACTATAATTATTGGAATTTGATTCTTAATTCATGAAATTATGACTTTGAGCATTGTTTTTAAATTTTTTGAGAACCAAGTATTTGAAAATCATAATTTTTTTTATTAATTTAATTATTTACAAACTATGTAACAGGGAAATAAAATTTAATTCTTCAAAATAATAACAATTTTTTCAAATGAGAGTAAAGACTTTTCTTATTTGAAGTATTATCTAAACATACATTATTTCGTTTTTCTTTTCCCATTTAACAAACAATTATTTACAAAAAATTATAATTTGAAGATTGGTAAGAAGATTCCGTCAACAAAATTTAATTTGCAACAACTTTTGTTATTGATATGAAATGAATATTTTCCTATTTAAACTATGTGCAAATGTATATCTAGATAATTTTAAATTTCCATTAATATCAATATTTGACAGAAAAATTATAATAAAATTTGTGTTGATTTTTTTTAGGTTTATACATAAAATAATTTATTTGTTTTAGTAAATTTAAAGAATATATTTGTGTAATAACTTTAGTAAGTCGTGATAAACATATGTATGAAAGATATATCTTGCATTCGTTTTAATATAAATATTGAATATTACACAATTGAAATATGCAAACTAGTTTAATTAGTACGAATAGTATAGGTTTCATTTTGTGAGATTTCATTCAATATAGTGTTGTTAGGAAATTGATTGTTGAAAAATGATAAATACATAAAATATAATTATACGAGAAAGTTCAAACAAGTAGCACTAGTGGTCTAGTACTAGAATAGTACCCTGCCATGGTACAGACCTGGGTTTGATTCCCGACTGGTGCATTTCAGAGCAATGGCATTTACTTGCACAAATTGTGAACGATTGGGTCGTCATGGTACATCTGATTTATAATATGATATAATGTGTATCTGAGCTCTGTAACTTTTACTCTTTTTTTTCACGACAATAATAATAACAATTACACCCAAAAGGATAGTGAGGTGAGATTGTCTTATCACAAAACTAAGGATCAAAAAAAAAATTATATTGTACAAATTTGAAAATAGTAATGTATATTTGAGTTATTTGATTGTCCATTTTTTAGTTTTTGTCACAACCTTTTAGTTTATGTTGTTTTAATTTTTTAAAAAATGTTAGACAAGTATATGTTGAATCCTCTAAAAATAATATATTTTTAAAGAATAAACATGAATACCGAAATAGTTTTTGTTGTTAAATTTAACTATTCAACCAATTAAACTTTCAAGTACATCATAATAATCTTTATGCATAAAATAACTTATTAAGATGCACATGAGAGCGAAGATTATCAACTATAGCCCATACATGTCTTCTCAAAGATGAGTAAAAAGGAGAAATGCAACATCAGAGTATATATACAATAGTACTTAAAGAAGTTTCAAATTGAATTTTGGTGACGAGAGACGTCTTTGTGAGCTGTTAAACTTCATTGTAGGAAGAATTTTGCGTGAATAATTCAATCTTCATTGAGATAATTGAATATCTATTAAGATTTTTTTTATCATCTTTATAATTTACGATTCTCACACTTATGCAATTTAAATAACGATTCTTCCCCTCCGTAGTCGATATTAATGGTGCTTTTCTCTCTGATTAATTTGTGCACTTAATAATTTTTTTGGCAAGAAGCAATGGTTATTCTTGATATGGTTTAATTTTAAATAGGGAAATTGATTAATGTGAATCTTTAATGTTTTAATTTTGGGATCATCGTATTTATAGAAAGTTGGTGATTTTTTTTGTTTTAGTTAACAAAGTTGGTGGATGATACTTTAAACGTGAATTTCACATTTAATTAGCTTGATTTTCACATTATTAATATTATATAATATTCATTGTACATTCAATAATATATAAATTGACGCATAATTCAATAGACTATTAAAAAATAGCATGTATAAAATATATATTTCATTAACTATAGTGATTGGAATTGTGAGTCTTATATAGTTCATAAAATTGCGTTTTCGAATTTTTCGAAAACCAACATGTATTTTGAAAATAATAAAAAAGTAAATTAATTTTATTTTTTTTACAAGCTACTATAAACTAAGAAGGAAATAAGCTTAATCATGTGAATAATAACACTATTTTTCAAATAGGAGTAAAGACATTTCTCATTTATATTATCTAAACATAATTTTTTTCATCTTTCTTTTCTTATTTAACAAACACGTCCAACAAAATTAAACAAAACTATTTATGAAAAATCATAATTTAAAGGTTGCTAGTGATTTTTGGAAATAAAATTATCTAATCATTTTTTTTCTTCAAAAAATCTCAAACTTAAAATTATAAGAAACAAAAAAATTAAAAAGAATAATTCGGGATAAATAAATGATTACTTTTTCAATTTCATATATTTGTTGTCGATGAAATGAAATGAAATGGAAGTTTTCCTATACCAAAAATAATTTTAAATTTCCATTAATATCAATATTTGATTAAGCATGAAAATAAAAATTAAAATTTGTATTGAAGATTTTTTTTTTTGATTTTATATAAAAATAATTTTTTAAAAGTAATTTAAAGAATATATTTTTGCCACGTGTTTTTCCACTGGTAATTGCCTAACATAACCTTTCCATATTATAGTCTTATCACGGGAGCTTATTGACCCTCACACTAGACAAAATCCGTCAGTTCAAGTGTGCCGTTTCACCTGAAATCGTCAGATTCCGGCGAGATGGCCGTCGAAGATTCGCCGGATTGGCCTCCTCCAGGATGGACAGAGGATGTCAAAGTATCAAAAGGAAGAAAAATCAAGGTAGAAATTGGACGTATTGAGCTAAATTTTACTCATCATTTCTTCTACATTGATGAATCTTTATAGTACGTAATTAGTCGATTGTATGGTCAAATACGAACAGATATATGTAAAAATTAGGATATTGTTGCTGTTATTGTTTTTTTTTGGGGATATTGTGTATAAATCCAGATGTGTTTGCGGTAATTTTTCACGGGGTCATCGGAAATTCGGATATTGTTGTTATTGTTTTTTACTATTCGTTTTTGAGCAACGGCTGATATGATGGGACAGCTCTGCAGCTCAATTTCGTTGTCATTTCTTTGAATTCGATATAAAGTTTAGTCATTTTTGGAGGTAAGTTAAAGGAATTTCGATTTGAAGTTGATAATAAGACGATTCTGTAGTTAAATCCAGATGGATTTATGGTGCTTTTTGATGGGGTTGTTCAAATTTGGGATATCAGCTTCGTAATATTTTTTAGTGCGGGATCACTTTGACAAACTACTTGCATGTAGAGTCTTGATATTGAATGGTTTATATGTTGTGTAACTTGAGTATTGTTCTGCTCTGTATTGCAAATTCAGAGTTTTAATTCAATTTTCCGCTTTCTAGCTTTTAGTGTTGAAGCTTTAGGTGGGCGAAGTGGGATTCAACTTCTTTTGTTCTTTCGTAAAACTAAGTTTATGAGGTGAGTACAGCAATGGTGTCTCAGGACCTGTGTGGACATAGCTTAAACATGGTAGAAAAGGATTCATATATGCATATCAACTAGTCATTATAAGGCCAGAATCATTTTAGTTTGGTTAGGAAGTTATATG
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 29
GEN 3040
TCAGAGTAGAGGTGTAGCACGGTAGCAGTAAGCGCAAGGGTTAGAGAACCTGCAGTTGTACAAAACCCCTACTTTGCCTTTAAAAGTTTCCTCAGTCCCTGAATTCTTTCTCGATGAGTTTCATAAAGAACTTCTCTTTGGAATACTTCATGTAATTGTGTTCCCGTCTTGTCTTGGCTAATGTTGATGTATGCAGTGATGTGTAACATTAGAATACAATAATATTCCCAGTGAAATCTCACTTGTGGGGAGCCGTAACATTAGAATCTGCACTATAAAAGGTTGTATCACATATTGTTTTCATTTATCTTTTCAGTACTATACAAATGGGGAGACTGGGAAAAAGTTTTATTCCAAGAAGGAAGTAGCTCGGTACTTGAAGACGAAAGATACTTGCGATGATGTAACTCAAGCAATGAATATTCAAGGTAAGAGCTTCTCTGAGAACAATGTTAGTGATATGGATAATCAAGATGGGAGCTTTGCCAAGGACAATGGTAGTCAAATGGATAATCAAGATAATTGTTGCTCTGAGAACAATGTTAGTCAGGTGAGTATTGAAGGTGACTAAAGCAACTCTTACGTTCTACTGTTTTGCTATGTGATTCTATCTTCTAAGGTCTGTCTCGTGTTATTGTCAGACTGTTGGGAAACCAAATAATTCTCATGAATGGTTACCTCCAGGGTGGATAGTTGAATTGAAGACTCGTAAGTCTGGTTCACATGCTGGTTTATCTTACAAGGTATGAAAATGGAAATATACCGACCTCAGAAATGCTTGTGGTGATGATTTGTTATAATATGCGTCTCTATTTTGACATTAGTGTGACCTTTGGTAAGATCAGCATTGTGTCAATTTTGGTGTTGTATCAGCACGTCTTTTTCCATGATTCCATGCTTAAAGTAAACTGATAACCGTGCTCACTCAAATTCCATGTAGATGACAAAGACTGTTGCATACAAATGATCATATGCTGGTTTTGGTCCTTCTGTTAGTTTGTCTCAAACACAAAATTCCAATAGTATTTGTTAGTTGAGGTAATGATGAGCATGCAGATTCTAGGGGTTGAGTGGTGCATAATATAGAGAAAAGGTCCAAATTTACCCCTTTACATACAAATAGAGGGGTAAATTTGGCATTTACTCATTTTTTTTACAAATATTTTCCTTTTATAAAAAAAAGAAGAGAAGAAGTCCAAATTTACCCCTCTACCATAAAAAAATATCTTAATTGTACTCTCGGTTATACTTTGGGGCAATTCATACCTGATACCTTTACCACTAATAACATGTCTTGTTTTTGCCCTTGATTTTAAGGACACTCCAGCATGGGCTGGGTGGACTCCACATGCCAATATACTTTTGGAAAATGTTCAAATTCACTCTCTACTTTTGACTAGGGTTAAAATTACCCTTCCTTATACATCAGACTGGAGTTCCTCTGTTAGGGTCAAGGGGAAAAAAAAAGAAACGAGATTTTCATCCTAAAAAAACACACATGAGGATTTGCTAACAACGGGGTGAATGGTCATTAGCCCTCACACATAAAGAAGGGTAAGCTACACTGTCAGCTTCATAAAAGTCAGTCCCATGTTTCTATAATTAGAAATCACTTAACTTCAAAAATTTTCTTCTTAACCTTAATGAAATGATTTGTAACTTAACTTTAAATTTTTCCATTTACCCTTAATGCGATAATTTATAGACATACAAATGATTCTTTTTTCACGAAAAAAGTGTCTAAGGTTTGTTTTGTACCACAAATTTCAAAAGTCTACATATCTTTCTTTAACTTTGTGCCAAGTCAAAGATGTGTCACATAAATTGGAACAAAGGGAGTAGTATTTTAGAACTTTGTTAATAGCTGTATAATGTCTTTTAGCTTAATCAAATTTGGTCCTGTATGTGCATCAAAACCTCAGACCAGTTTAGTCATCCAGCCAACTCCTGATTCAGTAATCTGCTGCATTTTTACAGGGTTTGATAATAAGGAAATACTATAGAGAAAACTCAAATTGAAGAAGCTTCATGACTTAACTTCAATTATACAAATTTTGACTTCTTTTGTCATAGGAACTTCAACCAAGTATTCCTAAAAAACTTCTTTAGGGGCTTCTGGTACCTTGGCTGTAGTCCCCCTTTTCTGTGCAACAGTTTTTTTGCTGCAACACCTAAGCAGCAGTTGTTCTAGTTCTCGACTTCTCTGGAAGAAGCATAGCTTTAGAGCAGGAGTGATGCTACTTTGCTGATAAAATGATCCAATCATACCTCATCTTTAGTTTACTAGTTTACATCCTTGTTTCTTTTGTTGTTCTTTCAGGTTTACATTGATCCATCAACTGGAAGCAAATTTTATTCAAAGCCTGAAGTATCTAAATATCTCAAAACTATGAAGCAGAACAACATCGCGGGGGAAGGACAAACAGTAGGGTCTCTCCCTTTTAACTTTCTCACCATGATAAAATCAGTTTATTATTTTCCTTAATTTTTTGGATCATATGGAGCCACATATTAAAGGTGGTGCAGTCAAAACCAAAATAAAGAAACGGAAGTACTTTGGATGAGATTTGATCTCTTTATTGTTATCTTGATCTGCGTAATTCTAATAATCTGTTTCTAAGGATATATTTAAGAGAAAAGGGAAGTTGCTTGTATTTATTTTGGCAGTGGCCTGTTGGAAATGACAAAAGTACTAAAAAGAGTTGCACCATGCATTTGTAACTAGTTTGAAATAAAAATCTAGCTTTGGGCAGACTGACTCATTTTTTGGACATTCCTAATAGGAAAGAGTACTTGAGTAGGTAAAAGTTTGATGGAGGAGGCAAGTTCCAGAAAAGAACTTGATGGTAGCTTTTGTAGCCTTAGGTTATTACATATTTTCCACATTTCATGTAGAAGAGAATTTAAAATGCCTTACTTAAGCCGAGGGTCGATCAGAAAAAATCTCTTTACCCGCACAAGGTAGGGGTGAAGTCTGCGTACACACCACCCTCACCAGATCCGACTTTTGAGCCCAGACCTCTTACCTACCCTGGGTATTTTTGTTGTTGTTGGGTTACTACAGCATCTTCATGTGTAATTATGTTTTTGTGTCAGCATGGAGATGGTGAAATATCTACTACAAATCAGAAAAAATCAGAGGATCCCAAAATTATCAGGGGTGGGGGTCAGACTCGCAAATCCAAAAGGCTGAAATCTGACACCGACCAGGCCTCGGTAAAGACTATTTTTTTCTGTAATGTACTTATGATACTGCTTACAGATTTCCTTCTCAAAAAAAGACACTGCTCACTGATTTGATTGTTAGCTATGTAGTTCACCATGGGTTACCGATTTCTCTAGTAACAAATAGGTGGCAGTTGAAAGCATTTCACTGGGTGTTGCATTTAAGAACTGCAACTTATTCCCGTCTTATTATGTTTTTTCTCAGCCTGGCATTGAGGAGGGGTCATTTTCCAAGCTGAAAGCATCTCAGGATTTTGAAGAAGTTGGGGAACAGAGTTGCACAACCAAAAGGAAGAAGTCTGGCATTACCAGGACTACAAAGACTTATCCTTCTGTAATATACTTAAGATACTTCTGAAAAAAATTGATTCTTTAATTCACTAGTAATATCTAAGTTGCTGTTGAAAGCAATTCAACTGGTGTAGTCTTGAGATGTTGCCAGTATTCTCATGTAATAATGTTCTTGTTTCAGCATGGTGCTGGAGAGGTCTTCATTTTAAATGAGAAAACATCTCTGGGTTCGAAAGAAAATGGGGCACAAAGTCCCCAATCCAAAAGACAGAAACCTGGCAGTGGCTGTCCGTCTGCAAAGAGTGTTTCTTCTGTAATACCTCAGATGCTTCTGATAGATATGATCTATAGCTGTTCTATTCACTACGGATTGCTAATTTTTGTAGTAATATTTAGGTTGCAGTTGACTGTGATTCAGCAGATGAATTACCCCCTGGCTGGAAAAAAGAAAGTAGGAAAAATGATCGTGGAACA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 30
GEN 3040
AGGAATTACCTGGTATTTCTCTTTTTTCCTCAGCCCCTCCCCCACCCCTCTTACTATCTATCATCTATGTATGTGTGTGTATATACAGGAATTTGCGAACCTACTACCTGAATGTAGTACTTAGCACCATAACCATTTTTCATTTTCCCAATATGAAAAGGGAATTGATCTATGGTTCGAGTAATTTTAATTTCGTTCATCAATAGAAAAGGGGAAGTTAGAAAGGATTTTTTGAATCCATACGTATTTAGAATATGTGCATCTTTTCATAACAAAGATAAACTTTTTGGAAATATTGTCTACCAAGGTCTTTATTGTTCATCTTCAACTCCAACAGACTTTTCCCAATTGAAAGAAACCCATCTCATTTACTCATTTCGTCTCCCCAAAGACCACCTCATGCTAAAATGGGTTCGTTATCCCTTCCATGGTCAATCTTGGATGTGCTAATTGAAAGGCTAGAAACTAATCGAAGCCGTAACAGGAATTCCATTGAAGTAGCAGGGAGCTGGGAAAAGATTGTGGGGAGTGGGGTTGGGGAGAAGAGACTTCAACTTTTTTGGTTAAAATAAACGTCCCTTTTTTAATGAGATAGTACAGACAATTTGGGTAAATTAAAGTGGATTGTAAACCTTTGCACAACCCATATTGTACATACAAGTATAATCTGTTTAAGGGGGACATTACGTTCTGTTTTGCACCTTTATGCAGTATTTTAGCATGCATGAGAAAGGGTGCTGTGGAAACAGGGTTAGGGGACTCCTTGTGGGACTGATGTGGAATGGTGTGACAGTAGATATATATTCAAAGTTGAGAATTTTATGTCGGCTTGGTCATTGCAGCTCTACACAGATCCAGTACATGGATATAAATTTCACTCCAAAAAGGAAGTTCTCCGATATCTACAAACTGGAGATGCTACTAGCTGTGCTAGAAGACCTACGAAAAGGAATGTAGCTTCAACTACAAAGGATGATTCTGTAAGTCACATCTTTCTGGATCCCCCCTCCCTGTATACCCATCACTGCTGTTATTTGTACTTGTTTTATAGCCCTTTTTCGTTTAAGTGTTCAGCTTGCATACAGAACCTTGTTGGTCATCTTTGTAATGTTGCTTCGCCCTTCAGTGAGATGCACCAGTCTCAACCCTTTAGTAGGAAAACACATTCCATCCTGTAAGAGTAGTGTATTTAGTGCCTTCTAAAGCAGGCAGGCTCGTTGAAGTTGTGAAATTTTAGTCGCACTTGAAAAATCCAAGTTTGAAGAGAATAATTTCACATCTTATTATGCAAATTGAAGGATGTGTAGACAACGAGGGCATTCGGCTAATCTTATAAAATAGTTAAACTGGCTTGTAAGCACCTTTTGGCTTATTTATGCATTAGATAGCAGTCAAAGTGTTTATAAGCCTTCCAAAATTAGCCAAAAACTGTAAGTTGCTCATTCCCAACTTATGGCTTTTCAATTTGTAAGTACTTTTGGTTTGACTAAAGTTTTATGATTTTATCCTTGATATATTCTCTGATTCACCAAGTACCTTGATAAGATGGTCTTTTGTTGTTCGTCCTTTCCCCTACAAAATATATATTTTAATATATGAAATATATTTGAGGTAGAGGGTCTTCAGAAAACAGCCTCTCTACCTCCTCGAGGTAGGGGTAGTGTCTGCTTATACTCTACCCTCCCCAGACCCTTCTTATGAGACTACAATGGGTTTGTTGTTGTTGATCTTTTGTAAAAAGGTTGTGGATATTTTAGTCATTTTAAAAAAAGACCATCTTATCAACACTTTTTTACCAAACACATTAGCTGTTTATTATCTTTCAGCACTTCTATCCAAATACGTTTGTTTTAAAAAAAATTCCTTCAGCACTTAAAAATACTTTGCAGCACTTGAAGCTTATCAACTATCTACAATCTGCTAATCCAAACAAACTCAATAACTTGCAAGAAATTTAGCTAGTGACTTGTTTGGTGGATGGCAAAGGAATGGGAGAATACCTTATGATTCTAGATCTGAACCATCATCCAAATGCTATTTATTATCTTTTTCCTTGTAAGTCCATTGAATGAGGAGGAATCTGTCTGGTATTGCATATCAAATTTAATCATTCAAGTGTGAAATCGAAGTCGAATGGAAGAAGGGCATCCAAATGTGCCCAAGGTGTTTTTTTTTTTTGTTTTTGGTGAGCAAAAGGCAGAGATAATCAACCATCTTTTTTTACATTGTAAAGTGGTTAGCCAGCTATGGAACCTGTTTATTAGTTTCGGAGGCATAAGATGGACTATACCAGGAAGGGCAGGTTAGGCTCTAGTAAGCTAGAACAATGAAGGGAGTGGTAGCACAGACAAAAACATATGGAAAGTTGTCCCAGCAGTGATATGGTAGACCATTTAGAACGAGAGCAATTCAAGATGTTTTGGAAGTATCAGTAGTCCTTAAGATTAAAATGAACTGCAATATCACTTTTTGCTATTGGTGTTTCTGAGTATATAGATGATCCTCTAGCAATTGTAGACATTCTATGATCCTTGTAAGATGAAATAGGACCTGGGATAGGGCTTTCTTTAAGTTGCTGAGCCTATTCAGTTGTATGTAGCTCTGTGATGTAAATTTTTGGGAACCCAACCTTAGTGCTGAAGATTTATTTACATAATTGTTACCATTGTCAAAAAAATAAAATAATCAAAGTTGAAAGACTTTTCACCGTATTAACATTTTGCAGATATGGGTATATTCTTTCTGTCTAACTGGTCGATCTTGAAATTCTTCCTCCTTGTTCTTGACTAGCCTTATTTTAGTTTCATGAAAAACTGTAATTGGTCTTGTTCCTTAGTAGACATAGTTTTTGAAAAAATTGTTGTTTATGAGCTTAGTTTGCTTATTATCAAGACTAGTGGATGTTGATTTACCCTAATGGGAGGGATCTCTCATGTTTATAAATTTATTTCTCTTGATAGCCCACAACAGATGATGCCAGCTTGAAGAGAGTAGGAGGCTCTATGACAGGAAGACAGCTTTTTTCCACTGACGAATTGAAAGGTGATAATCTGTAGTTGGAAATATTGCTCTGTAGTCCCTATCTGAGAGGATGTGCATCGTTTATTCAGCGTCTGGTCATTTGTTCTTGTCATTTCAGTTCCCTTCATTTTATTTTTCTTTGTCAATATGTTCACCTGCTCTCTCTCTAGATGTTGGTGATTGTTTCTCAATTGTGAAACTGGGATTACTGATGTAGGTGGGGAAAGTTTTGGTACTTGCTCACCTGCACAGCAAGTTGAGAGTTCGAAGAAACAGCCCGACTGCAGTGTGTCTGATCCTAATGCCATCATCACATCCATTTTAGCTGAGATAAATGAGAATCATTCCTTTGAAAATGTAGTTGGAGATGCTGAAATCGAAACTGTAAGTGTTGGTGTTAAACAGCCATCAGCTGTTTCCACTGAGTCGGATCTCCTCTCGGAGAAGCAACTACTAGAAAATGAGCAGGAAAAACATAGTTCTAAAGCAACACAGCAGTCAAAAAAATCCAGAAAAACAGAGTCACTTAACCTTGGTCGTCGGGTCTCCAAAAGACTTGCAGGTCAAAATGCAGAAGCGGCGGCTGATTTGGATCTTGGTGAACGTGCTCTTCCAGCTGTAGCTGATAAATCTGCGAGCCTCTCCGTGAATGCCTGCAGCCAGGAATTGCTCCAGGACTCCGATCCTACACCAGAAACTGGCAACTCTGATCAGGCTTCTTTGAATGGAGACCCTTCATTGGATGATTTACCCCCAGGCTGGAAAAAAGAAATCAAAATTACTAAAAAAGCTAATGGGATAAGAAAAGACCCGGTACACTCTCTCTCTTCTTGCTTGCACATCATCCTATTCCAGCAGACTATTTCGCATCATCATGCCGTGGCAGGTTATACTTGAGAAAGGCTGTGTTATGTTAGCAGGAGTAGGAGCTGCTTATGTGAAGAATGCTGCATAAGTAGATTTACATTC
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 31
GEN 3040
AAAAGATTTTAGTGCGGATGTTAAATTGATTCTTTTATTGCAGTTATACATTGATCCAGTGGATGGCTATGTATTTCGCTCCAAGAAGGATGTTTTCCGCTATTTACAAACTGGAGACATCAGTAGTTGTGCTATAAGACCTGTCAAAAGGGATCTAGATGCTGCAATGACGGATAGTTCTGTAAGTCATCATATTCCTGGTTCCACCCACCACAAAACACACACACACACCCCTCTGTTAGTTCTTTTTGTTGGATAAGTTCTTTTATCTACGTTACTGCACCTTCTGTTTTCAGTTTGATCAGTTAACGTTATCAATGATCTTTTCTTGTAAGTTCTTTTAAATGAGAAATATAATCATTACAAATTCTAGTTATAATGATATAGAAGGTTAAAAAGGAACAGCTAGATACCTCATGGTTCCCAATTCTGAACCACCATCAATGGAAATTGACATTGTCATTTCTCTTGTAAGTTTTTTAATGGGGAAATCTTTATATCAAGTCTTTTGCATATCAAATATAACTATTCCAAGTGTAGAATTTAAGTTGATGTTGTCATTAGAAAAGAATTTATGTTGAATGTCTTTTCATTGTATTAACATTTCAGTACATGTCTTAACACCAAATGATGTTTGAAGCAAAAAAAAAAAAACACTATATGATAATGGAATAATCTTTTTTTTTATGAAGTAGTTGCATTAATGGCATCAAGCAAATGCAACAAATATATTGATTACAAAAGAGAGTTTGTTGAATAAGTTACATGTAGTCCTATTTTAGGATAAGGAGCTAACTTAAGTCATAAAAATGGTCAGAGGTGTAGATAGGGGTATCTATAGGAGATAGATTCACCAATTAAATAAACATGAGACTTCTACCCATAAGGGAGTGATTTGGAGTTGTTATTCCATCAACACATCTGTGGCTCCTTTCTGTACAAACACACCAAGAAATGTTTGCATGGATCATTTTCCAGATTTTCCTGATTGATTTACCAACTTTCCCCCCTGCTGTACTCCAGCTTGGCAGTTTCCACTGCCTGTCCAAGGTTGAGCCCTGGGATTTGATGTTGGACTTAAAAAGCCGAGATCTCCATCATTCATAGGCATACTTAATGTTCTCCGGCATGGCCCTGAAATTTCCACAATTGTTTTGACTAGTCCTTTAATTGGGACTGTAATCACAAACTATGTGTTTCATCGTTTGTCATGAGTTTAAACTTTGACAAGCTAATTCTTTGAACTCAGGGCTAATGTATGTTGATTTACTTTGAACAAGAGAATTCTCTAACGGTTGAAAATTCTCTCTTTTGGTAGCCCACAACAGTTGACACCAATTCGAAGAAATTAGTGTGCTCTGTAACCGAAAGGCAACCTTTTGCTGCCAAGGAATCCAGAGGTGATACTTCTTTAGTTAATATTCCCTAACATTCTAACTATTAGAGGTGATATATACTTAACTAAATGTTGGGGCCATCAACAGCAGTCGTTTCAATTTTTCATTCTAATCTTCTCCTTGATTTTGTCTTATCGACTTTTTAAATATACATTTTTTTCTCAATTCTGAAACTGGGATTGCTGATGTAGGTCGAAAACGTTCAGTTACTTGTTCACCAAAAGTACAAGCTGAGAGTTCCAAGAAACAGCCTGAAAGCAGTGCATCCAATGCAAACACTATTATTACATCATCTGAAGCTGATATTATTGTGAAGCAAAATGCTGAAATAAGTCTAGACACAGAGCCAGAAATAAGAGACTCAAGTGCAGACACAAAAGTCATAGATGCTGAAAGTAACGCTATCAAAAGGTTATCAGCTGTCTCAACCCCTCAGTCAGATCTACCCTCAGAGCAGCAATTACCAGAAAATGAGCCCGAAAAGCATATTAGTAAAGAAAAACCGGAGCAGTCAAGAAGATCCAGAGCTAAGAAACCACTCACACCTGGTCGGCGGATCTCAAAAAGACTTGTGGGCCACAGCCCAGAACCGGTGGCTGATTTGGATCTAGGTGAACGTGCTTTTCGAGCTGTTGTTAAGAAATCTGCCTCTTTGGGCATCCCCCTGAATGTCTCTGGTCAGGAATTTTCCCAGAACAAAGATCCTACAGTAGGAACTGGCAATTCTGATCAGGCTCCTCTGAGCAGAGAAGCTTCAGGCCTTGAATTTCCACCAGACTTGGGAAAGGAAATGCTTACCCAGAATGAAGATCTGACAGAAGGAACTGGCAATTCTGATCAGGCTTCTCTGAAGATAGAAGCTTCAGGGGATGGATTATCGCCAGGCTTGGAAAAGGAAATAATTTCCCAGAACAAAGATCCGACCGTAGGAACTGGCAATTCTGATGAGGCTTCTCTGAGCAAAGAAGCTTCAGGAGATGGATTATCACCAGGCTTGGAAAAGGAAATACTTTCCCAGAACAAAGACCCGACAGTAGGAACTGGCAATGCTGATCAGGCTTCTCTGAGCAGAAAAGCTTCAGGGGATGAATTATCACCAGGCTTGAAAAAGGAAATACTTACCCATTATAAAGACCCGACAGTAGGATCTGGCAATTCTGATCAGGCTTCTTTGAGAGCTTCAGGGGATGAATTATCACCAGGCTTGAAAAAGGAAATACTTACCCATTATAAAGACCCGACAGTAGGATCTGGCAATTCTGATCAGGCTTATCTGAGCAGAAAAGCTTCACTAGATGAAATACCACCAGGCTGGGAAAAGGAAATGCTTGGACAGAACAAATATTCTACTGTAGGAACTGGCAATTCTGATCAGGCTTCTCTGAGCAGAAAACTTTCACCAGATGAAATACCCCCAGGTTGGGAGAATCAAATACCCCCAGGTTGGAAGTATGAAATACCTGCCCAGAACAAAGATCCTATAATAGGGACTAGCAATTCTGATCAAGCTTCTCTGAGCAGAGAAAAAGCCTCGCTGGATGAAATACCCCCAGGTTGGGAAAAGGAAATACTTGCCTACAACAAAGATCCTGCAATAAGAACTGGCAATTCTGATCAGGCTTCTCTCATCAGAGAATTAGCTTCAGCAGATGAATTCCCACCCGGATGGGAAAAGGAAATGCTTACCAGGAACAAACATCCCACAGTAGGAACTGGCAATTCTAATTCACTTGATGATATACCTCCAGGCTGGGAAAAGGAACTACTTGTCCAGCAAAAAGATCATACAGTAGGAACTGGCAATTCTTATCAGTCTTTTCTGAGCAAAGAAGCTTCAGTGGATGGATTACCACCAGGCTGGAAAACAGAATTCAGAATAAGAAAAAATGCTAGTGTGATAAAAAAGGACCCGGTATTCTCTTTCTTCCTCAGCATTTATGTCTTCCCTTTCCTCCCGGTGCACTTCTCTCCCCCTTCTTTTCTCATGCACATCATCCTACTCTAGTAGAGTCTATTTACCACATACTAATTCATAAAAAAAAGAGTCTGGTTTACACCATTAGGGCATCTCCTACCCATAGCAAATAGTGTTGATACTATTTTAGTGTAAACCATCTCCAACCCACTACACTAAATTTTATACTAAAAAAGAATATACTCCTTATAATCTTCTCTCTCTTCAATTTTTTATTATAATTCATATTTTCAATTTTTTTAGTACAAATAATAATAAAATAATCAAAAAGAGATTTGGTGTTGATGAATAGTGTTGCACCAAATTTTGTGCAGCACTATTATCACACTAAAATAGTGTAAGAATTGGTGTCGGGTTGGAGCACTAAAACACAAAATTTTACATCAAAGTAGGATTTGGTGTAAAATTTGGTGTTTGGTTGGAGACGATCTTATGCAGATATGTGATATATGCATGAGAAAGGGTGCTTCTGGAGAAGGGTCATATAGCTGCTTATGTGGCAGACATGGAATGCTGCATTCTTATTTTGCAGTGAGAATTTTAAGTTTACTTGGACATTGCAGTATTACACGGATCCAGTGCATGGATATGTATTTCGTTCCAAAAAGGATGTTATGC
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 32
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GCTATCTGCAAACTGGAGACATCAGAAGTTGTGCTGTGAAGCCTACCAAAAGGGATCCGGGTTCGACAATGAAGGATAATTCTGTAAGTCTGCACCTTCTGGATCTCCCACCCGTGTTCCCCATCAAATCTATTTGTGCTATATGTTCGATAACTCCTGTTATCCATGTTACTGCTTTTCAGCTTGCGTAATAATCTTGTTGGTCGTATTTGTATGAGCCTTTTACTAGGAAGATATATTTGTTTCTTTAAGACCAGTGGTAAAGTTGGTGTCTTGTGTAAAACTTTGTTTTACGTATTGGAGGTGTTGAAGCACCTCTTCTTACACGGGTTTGAGTGGTCTGAGACTGAGCAACTGATTTAGAGAATACTCGTTGAGAAGATATATTGGAAAGCAATAACTCCAAGTTGAAATAGAATAATTCCACATCACTCTATTCTAATTACATATGTAAAAAGAAAAGCTTATACGAAACTACAGGTAAAGACTATGCCATAGAAGGGTAAGAGGAATGACTCTTACCTTATACTTCCCAAATCTGGACCACAGTTCTGAGCTTTATCCCTGTTTCTATAAGTTCATTAAATGTGGAATTTCTTTATAAACTATAGCTGCAAAACGATGCGCTGGAAAGTTAGAAGAATGACTCAATACCTTAATGATTTCGAGATTTCAACCACCATTTAAGCATTTTACAAGTCCCTTTTTTCCTTGCGAGTTCTTAAACAAGGAGGAAAATCTTTTATGTGTGGCGTTTTGCTTTAAATTAACATTTCGCTGCATGTCTTTACTTTCTGTTCCTATCTGACAAAATAATCTGGAAATCCTTCACTTAATTCTTGATTGGCCCTTATTTGGTTCAATAGAGGACTGTTGTCACAAATCCTTAATGCATTTCACTGTTCATGACAAGATTTGAGTTCGGCAAGGTCATTTTTAATTTCTCGATACAAGTGTATTGATTTATCTTGAAAGGGAGGAAGTTCTGATGGTTGAAAGCTTCTTTCTTTTGACAGCCCTCAACACATGATACCAGTTCGAAGAAATTAAGGTGCTCTTTGACCGGAATACAACTTTCTGCCACTGATGAATCAAAAGGTGATGCATTTTAGTGGGAATTTTGCTCAAACTCCAATACTGTAGGAGATACAACATTATTCAGTATCGACAGTCATTTCAATTCCTCCAATTTAGTCTTGTCATTGATATACGTCCTCCTGAATTCTCTAAAAGTTGATTGTTATTCTCATTCTGAAATGGGAGTTGCTGATGCAGGTAGTAAGTGTTCTGTTACTTGTTCAATGGCACTGCAAGCTGAGAATTCCAGTGAACATCCTAAAAGCAGTATGTTCAACCTCAACACCAGTATTTCATCTATTGTAGCCGATATAACAGAAAAACATTCATATGAAAATCTAAATGAAGATACTGAAATAAGACTAGATGTGGAACCAAAAATAAGAGACTCAGTTGGAAACACAAAAGCTGCAGCTGCTGAAACTGTTGATGTTAGAAGGTCATTAGTTGTTTCCCCCCAGTTGGACTTCCTCCCAGAGCAGCAATTACGAGAAAATGAGAAGGAAAAGCATAGTAATAAGGGAGTGCCAGCAAGGTTGAGAAAAATCAGAAACAGCAAGTCATCTACTCCAGTTCGTCGTGTCTCAAAGAGACTTTCACGCCACAACCCAGAAATGGTGACTGATTTAAATCTAGGTGAACGTGCTCTTCATTCTGTGGTTAACAGTTCAGCCTCTTCAGGCCTTCCCATGAATGTCTCTGGTGGGCAATCGGCCCAGCAAACTGATCTAGCAACTGGGGATTCTGATCAGCCCTCTCTAAGCAGAAAAGCTTCATCACGAGATGATCCTTTAGAAGTTGTGAAGTGTCTTTCAGAGGGCCAAGCGTTTAAAGAGAATATTGATGAGAGAATATGGCGAGATTCTCAGCCAGCTGGAATATCATATGAAAGAGGAAAGGTTCACTTGGAACAGCATGCGCTTAACGAAAGGCCTACAGCCAAGCCGGCAAAAGAAGAAGAAGACGGTCAGAATAGATTATTGAACAATTCTCAACTAGCTGAATCAGCATCAGGAAGAAGGGTATTCCCTGCAGAGGACCGGTCTGTCTCAGAAAGGTCTACTGTAGAAGAGGTAAGTGAAAAACGAAATGGTGAGTACAAGCAATGGCAATATCCACAACTAGCTGAACCATCATGGAGAAGTGTGGATCTTAATGTGGAAAACCAGCAATGGCAAGATGTACAACTAGCTCCATCACAGAGATTCGTGGATCTTAATATGGAAAACCAAGAGTGGCAAGATTTACGTCTACCTCCATCACAGAGAAGTGTGGATCTTAATGTGGGAAACCAGCAGTGGCAAGCCTCACAATTAGCTGAACCATCACACAGAAATCTAGATCTTAATGTGGATAGCCAGCCTGTTAAAGAAAAGCAAACAGGAGAGCTGGTAACTGAGAATCAAGATGAACAGAACAGAAGATTGCATGCGCAACTAGCTTCTTATCCATTTGGGGACTATTGGTCAGACCCATGCATGGAGTTTGCATTCAAGACCCTTACAGGTGCACTACCAGTAGAAGATACCCTGACCTTTCAGGGATCTACCCATCAAGAATACAATACTTCCTACACGCAGGCCGATGATGGATGTTTTGAACTGCCATTATTCAACACTTCCAGTTTTTACTTGAATGATGCTCCAAACCACTGTGCTCCATCGGTGGAGCACGTCGTCAAAGAGCAACCGCCAATAAATCAGACCTTTTTGCCCACTGGACATAATAGCATACCAGGCCACAGTAGTGTTGTTTCTCAAAATCCAGGTTTGACCTTTTTGCCCAATGGCAATAACAGCATACCAGGTTGCAGTAGTGTTGTTTCTCAAAATCCAGGTCTGAATACCCAGGCAAAGGACTACCAGTCTAAATTTAAATCTCACAGGTGAATGAGAGCACATAAAGAGAGGGAAGTGTTAGGTGTATTAATGGATGAACTAATGACGCGGTGCTGCTCCTGTGAATGCATTTTTATGCAGCTATATCCTCTGTTTGTAAACATTTCTCTGATGCGGTTTCTGGAGTTTGGCGTATCTATAATTGATTGAAGATTGACCACAGGTGATGCTTCATCAATTGTCTGGAGGTGACAGATAAATTAGGTTTGTTCGAACCAGATAGCTGGTCGAAATGTAGCTTTAACTTTCAGAGTGCTTAGATCCCACGATCCATTGGACTTTAGACGATCTGTTTCAAAAGACATTATAGACAATTGCTTGCTATATGTCTGTATAATTTAGGTTGGACCTCTAAGCTTTTATTACACGTAAATTCGTCTCTGTTTTCTCTCCACCTCTATGACTGGATGTTAAAAGGCATCCATCCAAAAGATGCTTCTTCAAACAGGTTACTTATTTGTGAAAAGATTAGAAATGTGCACTCAAACAGAGTGCAGTAGTATGCACCTTTTTGAGACCCATGTTCATATTCCAGTAGGTACCATAAAAAACTCATCTATCCATTCTTTAGGTGAAATAGTCGTGGCGAGTTGGATCGAACACCATCATATGTTTTGAAAATGAGAAGAAATAAAGAGCGTTAAGGACAAAGTTAGAGTGATTTCTTCACATCATATGTTTTGAAAATGAGAAGAAATAAAGAGCGTTAAGGACAAAGTTAGAGTGATTTCTTCACAATAATTTCTAGATATTTGACTTGATTATCTCATAACTTGACATTGTTGTTCTGCATAATAACAAACCTTACATTGTAGCATATGATAAAATAATTTAACTTGAAAAATGAAAAGAAAATCCCAACATTTTTATTACAGTAACCCGTATAGACTCAAAATAGCTCTTTTCTTTTTTACTATATGAAATCCACACTTTTAACACTTGAAAATGTTATAACGGGTAAGATAAGTTCATAATTTAAGCAAGAACAAATTGATCAGTCGAGTAATGCTTCACA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 33
GEN 3040
TCAATAAGAAGACTCTTTCCATCCACCAAGGTTCTCTTCTTCATACTCCCTCTCAGAATATTCCTACAAAAACATAGCAAAATATATCATGCACATAGAATACCTATTATACTTGCATGTTACGAGTTCCTTTATTCATATAGCCAATCTGAACTTGTTAGCTCGGGATTGAGGCATAACCTTTTTTTTTTTTTTTTTTATAAATCAATAAAATTTTAGAAAGTAACTTGCCTCAGGAGTAAAGGTGAGACCAACTTTAATTTCTCCACAATATTCCTCATCTTTAACAACATTATAACATGTTATTGGGATGCTCCCTTCTGAAAAAACTGGTTCAATTGGTATCCTGCAAGTAACATATTATTGAACATATCTCAAAACCAATACTAGCAAACTCAGATTATGTTTGCATATATATATATATATATATATACAGAAATTGTATATACAGAAATTGTATAAATCTTAGATTCGTCTCTGATAGGTAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGGAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACCGTTTAAACCATGGTTTGTATAACACCACTAGAAAGTAGATGTATACTACAACAACCACAAAAATAACTATATATTCAGTGTAATTTCACAAGTTGGATATTGGGAAGGTAGGATATCGATGTACGCTGAACATACCCCTACCTTTGCAGGTTGTTCACGAAGGACCCTGAACTCAAAAGAAAAGAAAAGAAAAAGCATGGTGCAAGTAAATAAAACGACAAAATAGCAAGATACAAATCAAATGAAGCAACTGATTGTAATAATCATTAAAGAAAGGAAACTACGCGATATACAAAATACTACTACTAAAAAGGAGAAGTAGAGACTAGGCTAGCCCTCTTCCTCTCCAATAACAAGTGCAACAACACTCAGGCTCACTAACTACTAACCTTCTACCCTAATTTTCGACCTCCACGTCTTCTTATCTAAAGTCATATACGTCTATTTATCTCAACATATACAAGTCATGGATGCATGTACTATCAAACTCAAACTATGTTAATATAGAGAAAATACGTATAATCTTAGATTCGCCTCTAATTAGATATACTTACTTAGCTTCCCCAACAATATCGTCCTCGCCGATAGAATCACTGTCCATTATCTTCAAGAAGAGTTCTTCAGCATCCTCAGAAATGGAGAAAACAAATGTTTCATTCCATTCTGGTTCACATCCCTTCCCTATGAAAATTTAACATTATAAATATCGATTGATCAAACTTCATTTCATTTTTCTTAAAAAAAACATAGGTAACTAATAACTAAGTAATAATTAACCAAAGATTATTAACAAATGTACCTGATGCAACAGTACTCTTTTTCTCCTGAGATCTATAAGTTAAGATCACATATGGATCCATATCATCTGTATCACACAAAATAACATTTTTTAAATTAAAAAATAAAATAAAATACAGAGTATCAATCAAATTAGCCAAGATGATTATCGATTTTTGATTCATATTCCTCGATTCTATTTTATTTGACATCGTTTGACTAGGCATAGACCTAAATGTCAACTTCACATACTTTGATTCAAAATTTAAAGTTTACCACGAAGTCAAATTAATATACATTGATAAGGTTTGGCCATGAATTTTGACAAATATCTGTTTGATCATGAAAATTTGATCCGATCTTCAAATAATTCTCTATTTTCCTAAATAAAACGCACATTCAAACAGGTGTTGTGTGAACACATAAATTACATGTAGATCAACCTCTAATTCACATAAGTAACAATCGAAAATTAGGTTGATTAAGAAAACATCAAATGTAATCGAAAGAAAAAAGAGATCTATACATTAATCTGATCAGATCAAAACAAATAAATTAGAAAAAAAAACAAAAAACTTACTGAGAAAATCAGTGTTTTCAAGGCCTTTAGCTCCAACAAGTTGAACTACAAGTTTTCCTCGTACCATTTTCAATCAAACTCTTAGCAGATTCCCAGGAAAAAAAAACAGATTCTCGCACGAATTTTCGGACAGAGATCGTTTTCTTTTTTTTTTTTTTTGTTTCGATGAAGAATATGTTGAGCTGATGTGTATTTATATGTGAACAAAAGACCGGTTTAAAGTGGGGTTAGACCGGTGAGTGTAAAGTGAACCGGACGGTTTAGGCGAAAATCACATTTTAGACCAAAAAAGGCAAAAAAGAAAATCACATTTTACGCACCTAAATTATTTCGAGTATGAAAAACATATTTAAACTAATAATAATAAGTTTGAGAAATATACTTCAAGTAATATGTGATGTCGTGTGTATTACGTGTATACACACATTTGATTATGTTAGATTGCGTTGGCGAAAAAAAAGCCACGTGGATTATTTTTTTTTTTGGATCAAATGCGTCGGAGTGTGCATTTGGTCTGGTTAGAATTGAATTGTGTTTTTTTTTCTTCAAATTAATGAGTGGTAATATTGTAGAAATGAATCAATAGAATCTTCTTCATTTTAAGATTTTAGGTTTCAATTATTCGTTTTTGTGACTGAAAAAAGGCTAATTCAGCAGCTTTTTGTTCAATTTCTCGTGCAGTCAAATTCTCATCAGTACAGGTCAAGAGAATGGACCCCTGGCCTCGGATGTCCATATAAAGAACACGACAAACATAAATATCCTCTTTCACTTCTATTCTAACGAACTGAATATCTTTTATAAGGAATCGGAGTACTTAGGAACATGTTTTATACATTTTCAATAATTTAAATATGATATTAAAAAAAAAAGATTGTTCAAATATGTTTTGACATTTTTAGCTTTTATTTTAATAAAATAGATTTAAAAAAAAGTATCTTTGAACTACGTGAAATTCAACAAATGTCTTCTTCAAAAATGCCATCCTATTAATATTCTTGTCCAAACATATCCCTACCATGAATATTTTAGTCAAAAAATATCCTATTACTACTTTTGAGTAAAAAATATCTTTTTAAAGTATTGTTTCTTTTCTGTTTAAACAAATTCCCTTTTTAAGAGATATTTGATATATACCCTTTGGGCCTTGATGGTTGATGAGGTGATCCAAGAAATATTTAGACTATCATTTAATGCTTGTTTAACAAACGAATATTAAGAAAGTGATTTAACATTTTTTCTTTATAAGATTTACCGTTTGTTTCAAAAATAAATTTCGATTTCACCGTTTAATAGATTTGGTAAAAAAAGTGATGATCGTAAATTTTTTTTTATGATTTATTAATATCACTCCCTTTTAGTGATATATCATACATTTCTTATTTTTTTAAAATGGATATTATGTGCAGATTATTATCTCAAGTGGACATATTATGACCAAGCAAAATTATTAGGTTTTGTATATGTTCATTGTACATAATAATTGTATATGCTTATAGCCGCCTATCTAGCTATATCTTTATAAAAAAATATAAATTATGGTTTTATTAATATATCAAGTGTCTATATATACATATATATATATATATATTTATAATATTGTCCATAGTGTAAAAAGCCGCCATACCCCTATATAAACCCCTAATTTATTGTGTAAATATATCAACAAACCAAATATTCTTCTTT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 34
GEN 3040
TATTCTAAAATGAGTCATTTGACAACTTGTTTAGTGTTCTTTCTCCTTGCCTTTGTGACTTACACTTATGCTTCCGGTGTATTTGAGGTCCATAACAACTGTCCATACACCGTATGGGCAGCGGCGACTCCCGTAGGAGGTGGCCGACGTCTCGAGAGAGGTCAAAGTTGGTGGTTTTGGGCTCCACCGGGCACCAAAATGGCACGTATTTGGGGTCGTACTAATTGCAATTTTGATGGTGCTGGTAGAGGTTGGTGCCAGACCGGTGATTGTGGTGGAGTCTTGGAATGCAAAGGATGGGGTAAACCACCAAACACCTTAGCTGAATACGCTTTGAATCAATTTAGCAACTTAGATTTCTGGGACATTTCTGTTATTGATGGATTCAACATCCCTATGTCTTTCGGCCCAACTAACCCTGGGCCGGGAAAATGTCATCCAATTCAATGTGTTGCTAATATAAACGGTGAATGCCCTGGTTCACTTAGGGTACCCGGAGGATGTAACAACCCTTGTACTACATTCGGAGGACAACAATATTGTTGCACTCAAGGTCCATGTGGTCCTACCGATTTGTCAAGATTTTTCAAACAAAGATGTCCCGATGCCTATAGTTATCCTCAAGACGATCCAACAAGTACTTTTACTTGTCAGAGTTGGACTACAGACTACAAGGTTATGTTTTGTCCTTATGGCTCTACTCACAATGAAACAACAAATTTCCCATTGGAGATTCCTACAAATACTCTTGAAGTGGCTTAATTAAGTAGAAATCTTGAGTTGAGCCAATCTCTTTTTTGTTAAATTTGTTTTGAAGATGGACATTGTCTTTAATTTGCCTTGTACACATTTCTTTTACATTTGTTTGTTTGAAGTGGCTAAGATCTGAATAACAATCTATGATCGATGCTTTGTCTTATATATGGTATTTTAATTTTAAATTGAAATTATATCTGGAGTACAGTTTTTTTCTTAAAATTTTTAATTATAATGAAAAAAAATTAGTCTAGTTAATATATTAAAGTACCTAGATCTGAAAATATAAAATTGTAGGGACAACTCGTTACATGTCATAATATAAGTCAAACAGATGGAAATATTTTTTTATTGGCGGCTTTTGGAACATTTTCAAGTATACTATAGTATATGAAAATTCATTAAAATTTTAATAAGTGTTTAGATTTGAATTTATAAGTTTAAAGAAATAATAAATTCAATATTAATTAAAAAGCTTAAAGATTCGAACATTAAGTTAAACTGATAATTAAATGAGTCGATATACAAAAAAGAAAAAGAAAAAAGAAGAAGATAATTTTATATAAAAGTCAACTTTTAAAAATCACTTACCCCATTAATATCTTCCTTCTAAAAGACGTTATCCTTTAGTATCTGAATTCATAACTTGAAATAGCAAAAAAGGGCTTTCACTTTCAATTGCGATTCAATGCCTAAGCTAAGAATTCAACATTATTTAAATATTGATATTGTATTAAAATTCATTACTTCCAAAACTCTTTTATTATAAAAAAATAAAATAAAATACAATTGGGCGTTCGTATTGCTTAAATAATTGCAATGTTACTTAAAAAATTCTATTAAAACAATTTAAGTTCTTTTATTCTGCCCAAGCAGTTACACGTTGTTGATTTCAGACTCCTCCGGTAGAGCCAACAACCATTCAGTGAATTGTCTTGTGTTACTAAGTTTCAAGTCGTTCACTGAATCCTTCACGATGTTTTGGTGGCAGGTACTAGTACATCTACCGATCAAAATCAAATCCAAAAACTCTTGCATGTTCCAAAATTAAACTCAAATATACAATCATCTCATTTAAACTCAAGAATATCTTATTATGAACCAACAAACAAAGACGACCATGATGACAACGATAAAAAATCAATACTCCCTCCGTCCAGAAATATTTGTCATGTTACGCTTATCGAAAGTCAATTTTGATTGATTTCTAAAGGTAAATTAAATCACATTAATTCGATACTTTAAACCAAAAAAATTAAATATTCTAAAACAATATGAAAAGTACTATAAATTACAATCTTTTCATATTAATATGATAAAAAAATGTATCTTCAAATGTTAGTCAAAGTTTTTATAGTTTGAGTCTATAAATAGAAACCATAACAAACAATATCGGACGGAGGAAACATAAAACAAAAATCAATATCGAACAAATTATTCAATCAATAACAAAAATCATTGCTAGTTCTATATCTAGTAACCAATTAGGGATAGATTATTCAAAACTTTGCATATTAGAGATAGATTAGTAACGAAATTCATAACTAATTTCAAAATTCATTTTTCTCGTGTGAAACAAGACCTACATAGCTTGTATCGAATAAAAAAAAAATTCAAACTTTTAAGGTTCCACTCATCTCTACGCCTACCCATTTGCTCAACTTTTTTGACTGTAGAATGCATGCACATTTTCAATTAATTATTATGTCCACTTGATAAATATGCTATAAATAATTTAATATAAAAATCCACTATATATATACGACGGAAAAAAATAATCAAGGGAAAAAAGGCAATTGTCTGTAAACAAAAATTACAAATTTGTAATTCAAAATTTTTAAGTTATTTTATTTATGAAAACATAACCAATAAAAATTATGAAGAGAATTCTCTACTAACTAAATTTCTCATGAAAAAATGTTCAAAAAAAATTAGGAAATTTTCCACAATTTTAGGTTTCATGCCCTGCCATGCATTTTTCCGATTTGTTGTATATACTAAACTAAATAATTTAAAATTAATATATAGTTAAACACAAAACTAATAATACGTATATATCACTTAATTAAAAAATATCTTCTCTTTTTTTTTTAATCATTATAAATATCATAATGTGTTAATGCATAAATTTTCAAAATAGCAAAAAGTTGAACTGTGAAATATGACTGGAGTGAACAAAATATATTACTTATAATAGGCTGTACTCATATTATATTGGCGGCTTTTTTTTTCAAGTGGAGATATTTTGGATACAATTTTTTTTTTTAATTTTCTATATATGTTTATAACAAGTGCCAATATTTATATGATTAATGTCCATAGTGAAAAAAAGCCGCCATACCACTATATAAACCCCTAGCTCGATATGCAAATATATCCTACAACTTCTTATACTAAAATGAGTCACTTGACAACTTGTTTAGTGTTTTTTCTCCTTGCCTTTGTGACTTACACTTATGGTTCCGGTGTATTTGAGGTCCATAACAACTGCCCCTACACCGTTTGGGCGGCAGCGGTCCCCGTAGGAGGTGGTCGACGTCTCGAGAGAGGTCAGAGTTGGTGGTTTTGGGCCCCACCTGGCACTAAAATGGCACGTATTTGGGGTCGTACTAATTGCAACTTTGATGGTGCTGGAAGAGGTTGGTGCCAGACTGGTGATTGTGGTGGAGTCCTGGATTGCAAAGGATGGGGTAAACCACCAAACACCTTAGCTGAATACGCTTTGAACCAGTTTGGTAACCTAGATTTTTGGGATATTTCTGTTATTGATGGATTCAACATCCCTATGTCTTTTGGCCCAACTAAGCCTGGCCCTGGAAAATGTCATCCAATTCAATGCACAGCCAATATAAACGGTGAATGCCCTGGTTCACTTAGGGTACCTGGAGGATGTAACAACCCTTGTACAACATTCGGAGGACAACAATATTGTTGTAATCACGGTCCATGTGGTCCTACTGAATTGTCAAGATTTTTCAAACAAAGATGTCCTGATGCCTATAGTTACCCTCAAGACGATCCAACTAGTACTTTTACTTGTCAGAGTTGGACTACAGACTACAAGATAATGTTCTGTCCTTATGGCTCTACTCACAATGAAACAACAAATTTCCCATTGGAGATGCCTACAAGTACTCTTGAAGTTGCTTAATAAGTAGATTCATCATGAGTGTAGCCACCTCTTTCTTGTTCAAAATGGTTGAGTTTTGTTCATTACTTGTCTTTTGTATTATC
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 35
GEN 3040
CACATTTCTTTTAGTTTGTTATCTTGAGGTGGCTAAGATTTCAATAACATATTGTACTTTAATTTGTCTTACACATGATGTTCAAAATTCTCACACAAGAGTGCATTTGGAGTATCGTTTAATTTTGTCTTTTTAATTCCTAATTATCTAAGTCTTAAACCTTAGACAAAAAAAGGAATGAAAAATAGTATTGATAACATTCTACCGTAATCAGATCAAAAATATAAAATTGTAGGGACAAGATAAACTATTATAATTACTATATAGGATAGCTATCTTTATAGGACATAATAATTAGTCGAGGTGGAAATCTTTTTTGTAGGCGGCTTTTAGAATATTTCATGCAAAGGTTAAGGTGGAGCCACGTAGAATTAAGGGGTTTATTCAATTTTTTAAATAGATAATTATTAATTTTATTTATATATAGTTAAAATTATTTTTAATGTATATACAGTGATTAATTAATGAATGAATTTTTTTTTTCTTTAAATTGTTAGTGTGTTTATTTTCTCATATTTCAAAATCCTCTAATGAAAATTATTACTCCGCTATTAATCATGCTCTTGATATTCAACATTTGTATTGAGCTTGATAAATTTAAATTTGCATTCGAAAAATTTCACTTTGAAGGCAAAACACTTATTATATATAATGAAAAGAAATTTACTCTCATGGCTCAAACTCGATATATCAAATTAAAAATTGAAAACTAACATCCCACCACAGCCCCTTTGCATATTTTCAGCACGTTATGGTGCATATTCCGTGACTTTTATGCAATCACTCCTGATAATAAGAAGCAAAGTCCTTAGCACCATAATATTCAATTCATTTTAAAATATTTATCATGTTTTATTTGAAATAACTGATCATTAACAAAATATGTTCAAATAATTTCACTCTTTTTTTTCTTAAAACTTAACTTAATAATTTAAATTCACATTACGTAAACTTTTTAACTCCACGCCATTATTATAAAAAAATATGTCATGTGTCAAGTGGATATATAATTAAAAGAAAAACGTATGTGTATCTACACTCAAAAAAGTCAAGAAAATGGGGGTATATGGAGATATTGGTGAGTGGGGTTTTTTATGTTACTTTGGTGGTTAAATAATTATTCATCATTACTTACTATATTGACAAAAGAATATTATTTTCAAGAGTAATGTTGTTAATTGTTTTAGGACAACTTTCGCATATAGCAAATAAAAAATTTATATTTGTGTGTTATAGCAAAATTTGTATAATTACGCTCCATAGCAAACATAAAACTGTATAATTCACTATAATATACAATTGTATAATTTGCTGGCCTATTTCGCCGCAATTGTATAATTCGCTATCATATTTCACTGCAATTGTATAATGCATATTGTATAATTCTCTGCAATATTTGTATAAAATTTATTTTGCATACAATTGAATCGAAGTAAAATGTATGTATATTGCATAATTATAAGTGTATAGGAAAAAAATATATGTTTTTCTCTCGCTTTATACAAAAACATAAACACAATTTATACACTTCTGTTGTATAAAGCAAGAGAAAATTGTATTTCACTGCAATTGTATAATTCGTTCGCCTTTTTCGCTGCAATATTTGTATAAAATTTGCATTTGTCTACAATTGAATTGAAGTAAAATATTTGTAAATTGTATAATTAAGTGTATAACACGAAGATATATGTTTTTGCATGTGTATATACAGTTTTCTCTCGCTTATACAAAACAGAAACACAATTTATACACTTTTGTGTATAAAGAGAGAGAGGCGACTGACAAACTTTGGCTAACGTTTGCTATGGTGCACAATTAAATCAAATCATAGCTACTCCATTTATTTTAGGTTATTAGTTTGCTATTATATACAATTATCCCATTATTTTATCCCAATACAATATTTAGTCATTTCATTCCATCAATTTCGGTGAAGTTCAAATCAATCGTTGTTTTAAAAGAGATTAGTATTGATAAAGTCACGTTCTACAATATTTTTTATTTTTTTGAGTGGCGATAAATTATTAATATTATTTTTGACAATTCATTTTATTCTTTAGAGTGCGATATAATCAACTTGTTAATCTATTATATCAAGTTAACAAGTTATTAGAATCTTTTTCACTCTTAATCCTCTAAAATATGTAAAACATGTTAATGATAAAAAAAAAATAGTGAATTTTCTACGTTTATAAATATAATTTTTTTTAATAAAAATATAAATTTTGCTCAATTTAATCTTTTGGCATTGTGTCAAGTAGCACGTGCAGCTAACATCATTTAGCTTGAAAATGTTCTAAGAGCCGCCTATAAACAAATATTTCCATCTACTTTTGACTTTGCCCTAAAATTGTGGATAATAATTTCTAATAAAATGGGATTATTTTATTTTATATTATTTTAAGGTACTAGTTACTTTTGAGATTAATTTATTTTATTTTTATATTTTGTGATGGAACTTTTATCATATAAAAAAATGAGATAAAATAATCTCGTGAGATGTTCTTATTTATCTTATCCAGTATATTATAGTTTTTATGTTTTTAAACTGACTGATATTATTCTTTTATTTGACAAGTTAATACGTAAATAACAAAATATACATAAAATAATATTCGAAACAACATATTTGTGATGATGTGACTTTTATTCATGTAATTATATTAGGTTTATTACAAGCCAATTATATAACTCGACTACTTCAAGCAATTTAAAAGAAAGTGACTCAATTTTACTTAGCCTCTTCATCACTTGAGGGCATCTCCAAGGGGAAATTTGGGCTAGTAACACCATTAGGACAAAAAACAACCCTATAATTTGTACTACCACTAGGGCAAGTAAATGTGCTAGTAGGATCATCTTGTGGGTAGCTATACGCATCAGGACATCTTTGTTTGAAAAATCTCGACAAATCAGTAGGGCCACATGGACCTTGTGTGCAACAATATTGTTGTCCTCCGAACGTGGTACAAGGATTGTTACATCCTCCGGGTACCCTAAGTGAACCAGGACATTCACCATTTATATTAGCCGTACAATGAATTGCATGGCATTTCCCTCCACTAGGATTGGTCGGGGCGAAAGTCATTGGAATATTAAATCCATCGACTAAAGAAATGTCCCAGAAATCTAGGTTGCTAAACTGGTCCAAGGCGTACTCGGCCAGGGTGTTTGGTGGTTTGCCCCACCCGGTACATTGCAAGACCCCACCACAATCACCAGTCTGACATGAACCTCTACCAGCACCATCAAAGTTGCAATTCGTACGACCCCATATACGTGCCATCTTAGTGCCCCTCGGTGCATTGATGACCCATGTTTGGCCTCGATCAAGACGTCGACCACCGCCTATTGGGGTCGACGCCGCCCAGACGGTGTATGGACAGTTGTTGCGTACCTCGAAAGTGGCAGCATAAGTGTAAGTCACAAAAGCAAGAAGGAAGAAAACAAAAGAAGATCTCAAGTAGGCCATGTTTGTGGAACTATTTTTTTAAATGTAAGTTGTTGGATTTAGTGACAAATTGTTAGGGTGTTTATATAGGGGTGTGGCGGCTTTTGAAACTATGTATATTAATCACAAATATCAATATCACAATTGACTAATCAAACGAATAATATTGAAAAAATACTTAAGTAAAGGTTGAGGTGGCTCAACATATAAGAGCCGCCTAGTAATTACTCCATTACTTTATATAAAAATAAATATTTAATTATTATTTTTTTCAATTTCTTATTTAATATTAAGGTATTTTTGTCCTGATTAAATTTAAATTTACAAATTAATAGCATTTTTTATAAGATTTTTTTATTTTCAAGATTTACAAATTTTCATTTCTAATTAAGAATCGAGAAATTTCGGTCCGCTACAATCCATATATGGATAACATAGGGACAAATTTATTTGGTATAACGTGATAAGACTTTTTCTTAACTTAATTAAAAGAAGTAACCATTATTTTTCTTTCATTGACAGAGTTTCTTTTGTACTCC
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 36
GEN 3040
CATTAAATTATTTTTCATTTTGTGTGATGTTTAAAATCATAAATAAAAAAAAAATAATTTTATTAATTCACTTTTTTAAAAAAGTTAAAAATTTATAAATAAATATGAACACTTTCAAAAGAGTCAATTACAAGAATAATATAAGAAAAATAAATTAATATTTTTTTATTTACTAAAATAATTAACTAATATAAAATCAATGGAGTACTTATCTTGTCATTTTTTATTTCGTGTTCAAGTTTCACGCGTATTATTTGGAGGCGAAATTTATGGATCGCAATCAATAAAATAAGGGAAGTTAATCTAATTAGGTAGCATCTAAAATCTCAATTAAAAAAACGAAAAATTACATTAATGACGTATAAAAACTTCAAATAATTACATGTTTTATATTCAATTCAATTCAAGAAATATTATTCTCTTAAAATATAATACAGCCCATACCCTTTCCCATTGATTAAGGTTGATTATTTTTGCTTAATACATATTAAATCAGTATTATATATTATATATTGTATTGATATCATTAAAATTTATAATTTCATTTAATTGATACTAAATCAAATATATGTATATATATATATATTGCCTATGTATTATCACTGGCAACCTAAGATCTATTTGTTATTAATTTTCGTCGCAAATTTAATTATCATTAATTATGGAGTTAATTACTTGCAGTGACATTATGGTCGCCACTAAAAATATTCAGTGTCGATTTAAAGAGGCTCATATACATGACAGCGTGAAATGATTCTCGAGGCATTATCAATACTTTTTTGTTTGTTTATTGGTCTAATTTAGGCTTAATACTTTTCTAAAAGTTATTTTTTTTTTAAAAAAAAAGAACGATAATTCAAATGCACTGTTGTTTTATACATCTAAGACAAAAGTACACATGAGATGTAACTCTTATTCCGGTCTTTAAGTTTGTTACGAACTCTTATCAATAAAGTAAAAGTGAATATTTAAAAAACTGAGCTATTAAGATTTTTCATTACGGTAGAGATCAGTTGACTAATTGAGGCGATTTAAAATGAAGAAACTCAAATTCACTTGGCCACTTCATCAGTACTTGCAGGCATCTCCAAGGGGAAATTTGGATCAGCAACACCATTAGGACAAAAGACAACCCTATAGTTTGTACTACCACCAGGGCAAGTAAATGTGCTTGTAGGATCGTCTTGTGGGTAGCTATAAGCATCGGGACATCTTTTCTTGAAAAATTTGGACAACTCTGTAGGGCCACATGGACCTTGGGTGCAACAATATTGTTGTCCTCCAAACGTGGTACAAGGGTTGTTACATCCTCCAGGTACCTTAAGGGCGCGAGGACATTCACCATTTATATTGGCCGTGCAATGAATTGCGTGACATTTTCCTCCACTAGGTTTTGTTGGGGCAAAAGTCATTGGAATATTAAATCCATCGACTAAAGAAATGTCCCAAAAATCTAAGTTGCTAAACTGGTCCAAGGCGTACTCGGCTAGGGTGTTTGGGGGTTTGCCCCATCCGGTACACTGTAAGACTCCACCACAATCACCAGTCTGACATGTACCTCTGCCTGCAGCATTAAAGTTGCAACCAGTACGACCCCATATACGTGCCATCTTAGTTCCCCTCGGAGCATTGATGACCCATGTTTGGCCTCGATTGAGACGTCGACCACCGCCTATCGGAGTCGATGCCGCCCAAACGGTGTATGGACAGTTGTTGCGTACCTCAATAGTGGCAGCATAAGTATAAGTCACACAAAGAAGGAAGAAGAGAACAAAAGAAGATGTCAAGTAGCCCATGTTTGTTGAAATGTATATATGAGGATAACTTATTTGAAAGCACTTTATATAGGGGTGTGGCGGCTTTTGGCACTATGTATATTAATCATAAATAACAATATCATATTTGACAAAAAACGAATAATATTAAAATACGATATAGTAAAGTGGAGGTGAAAAAAGAAAAAATGTATAAGAGCCGCCTAATAAATAATAATTTTATAAATATAGTACAAGTTCACTTTATTTGGTGACAACTTTGTAACGTGACGGAACTTATTTCTCAAAATAAATATTATTTAAAAAAAAAAAACAGCATCTTTCGTGACCAAAGTTTCTTTCTATATTTATCAATTCCATTTTTTTTACTTTGACAGTATTTTGTATACGTTTAAACATAAAAATTAGGTTTTTCATATTACTCGTATGGATCGATTAATTTCAAATATACAAATCAATTCGATTAACTATTATATACAGTGGAAATGATGTTTATGTGACTCGTAATTGATATTTTAAGCGATCAGAATATTTTAATGCATATCTATTAAGAAAATACCTATTGATGCAAAAGTCAACAAAATTATTTAATTTATTTATTTTACGATCGAAGCATGAATACATTTACTAATTAAAGTAAATTGGGAGGAATTGATGGACGAGTCATCAAGCTTATCAAATGTCAATCAACATTAAAATAATGTTAGTTTATGGCTGCTTTTAAATGAACAAGTGGATCATGTACAATTGAGTAAAAAATATCTCCTATAAATATATATATCCCTCTAAAACCAAATAACATAACACACAAATATAAACTTTAGATTCTTAAAAACAAAGCTGAATTATATGGAGGCAAATAAGTCTATGGTGAAGTTGGTTGCTTTCTTGATAATTCTTGTATCATCATGTAATTAATTGACCATTTATTTCTACTTTATTAAATTTATTCACATTTCTTATTTTTATTTGTTAAAAGAAATTGATTAATTATGTAAATTATTTATGAACAGGCTTTCAATCTCTCACTGCTCAAGATTTGGAAATCGAAGTTAGCGATGGCTTAAATGTATTGCAAGTACATGATGTGTCTCAATCTTTTTGTCCAGGTTTGGTTTTTATCTCTACCGTCCTTTTTGGATATATATACACGTACGACGTCCAACTAAATCTAAATAAATTTTTGGATCGAATCTTAGGTCGATTATCAGGGTAATATCTATAGATTCGAGTGTTGGGGTTGAGGTCATATCCCAATTTAAATGTCATGTTTCGAGTTGGGAGTTAGGGTCAGGTACTAGGTTAAATCCCATGCTCGGATACTGAGTCTGTTGTCGAGTTATGTCCTAATTCACGTGTCGAAATCGAGTTCCGAATGAATTTTCGATCAGAAACTATTTTCCTACTCTCTAGCCAAATATAAAAGATATTTTCTAAAAAATATTTTCCTTCGTAGCAAACACACCATAATTAATTATTTTTTTCTCATATAAAATAACTTTTGATTCATAGTTTTTTAGTCAATGCTCTTTTATAAATAATTAGTTGTATTTTTTTTTTATAAATTATAGGTGTGACAAAGGAAAGTTGGCCAGAACTTCTTGGAACACCAGCTAAGTTTGCAAAGCAAATAATTCAAAAGGAAAATCCAAAATTAACAAATGTTGAAACTCTACTGAATGGTTCAGCTTTTACAGAAGATTTGAGATGCAATAGAGTTCGTCTTTTTGTAAATTTATTGGACATTGTTGTACAAACTCCCAAAGTTGGTTGAACAAAATTAATTGATGTTATATCATATGTATCTAGCCTCCACAAAAATAAATTGGAGATGTATGGTTAAAATTTCCACTATATTTGGTGATAAATAAATGTGGCTTTTAATATTAATATGATTTTTGTTATATATACAATTACTTCTACTATATACCAGTGAAATCTCATTAGCATTGGGAAGGTGTTACGTACGCAACCTTATCTTATGCTAACTATCATCAGGGCGGTCTTAACGCTATGCAACTAAAGGGGGGTGGGGGGTATTTCTAAGAAAAAATTAATTATAAATGTTATTTTAAAAATAATTATATGTACTATGAGTGGTATGATTTCTACAAGGAAATTGCATATATAATGAGTATATCATCTCAATTGTGACTTTGATGAAAAGTTCTGACTTTTAAGAAAATTTTGTCTTTTATGAAAGATAGTGACATTT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 37
GEN 3040
GTTTTATCGAAAAAGGGTGGTGATTTTCGAAAAGAGTTTGTTTTTAGACTTTTAGGAGTCGCCACTTAATTTTTAAGAAAAATCAAGAAAACTTCGTTTCTAAACAACTTAAACAGAAAAAATTGTTTTGAAACATTTTAAGGGTTCGGGATTCCTATTAGTATCTTAGGAAGGTGTAAGGCACCCAAAACACTCCGTTAACTTACGGTTTTCCAATGATTAACTATTTGATTATTTTTATTTTTAACCTTTGCAAATTTATTTGAACTTTTAACATGACTTACTTAGCCAAACTTAGCCAATTTTGAAATATTTATTGTTTATCTTGCCAGTTTACGAAACCATTTCAAAGTTAAGCTTACTCAAGGTTTTATCTAAACAAGCCTTACGAATTTGAAACAATTTATCATTTAGAAGTTAGTTTTAAAAATTTTTAGGTTTGATAAAATAACAGGCGTTCGATGAGGGTTTGGAGTTTCCTAATCCTAAGCTAACGGCATTGAAAACAAAATAAGATAAGCAAATAGTAAAGAGAAGGATAGAGAGAGAGAGATTTGGGTCCAACTCCGGGTTCTGTTATCCTTGACCGAATGTTGGACCCACCTGCCTTTGGGTTTTTTAGCCCATTTCTGTACCTGTCCTAATCTAAACATACAAACATAATATAGAAAACAAAAAAACGACAAGAGGGCTTAGGCCCATTACAAAATATGCAAAGTAAAATAAAACGAAAGAAAAGTCTTTTAGTCCATCGTCTTCAATCTCCATGACCCTTGTCTTTCTACACTTGCTCGTTTTGATGAATTGACTCGATAAAGTTCAGAGTCTTCAAGACTCTCTCGAAATGCGAGGAGGATAATGGGATTCCCATAATGGACTCACGGGGAGTTTTCACATGTCACAAAAAAAACGATAAAATGAAGATTGGCATTTTTAAAACTACAATTAGAATAAGCAAGGTTCTAAAGCATAACAAGGATATCTTTATAATGCAATACTAATTAAATCTTCATGAGAAGCAAAGCCAACATCCAACAACCTTAATGGTACTTAGAGATATGCAAATCAGTCAACCTAAATGACACTACATAACGATACGTAAACTGTGCGGCGAAACCCCAAGTGTAGTGAAACCTTCACACAAAATTGTTGGTAATTTCCAAATTTTATATTTAAAGAAAGGACTTAGCATGGGATAAATAACGAATACAAATAGAGATGCATCATAAATTGAGTATCAATTAGCCGTTTTGCGCTTCAAACATTTGAAACAAACTTGAACCAATCATAAGAGGACAGCCTCATCAAATAAAACAGGATCCCGATCACCTTATCACCCAAACATCTAACACACTAAGCGAAAGGACCATTCAATCGACCTACTACATGGCTGTGACCTCGGACAGCTAACACAAGTGGCCACCAAGATAAAGATGGCCTTTCAAACCACAGATTTCCAAAATCCACACCACCCATACGGCATACAAAACGAAACTTTCATGCAAGACGCCTGACAAACATGATATCTAAACAAGATAACAAGTTTGGGAGATAAATACATTATAGAGAATAAACAACCCTCATATATTCTTAAAGCGATACTCGAACCAAAAGCTTGAAGAAGGTTCGATTGAAAAGAAGCTTACAACTACAACTCCCGCGAGATAGACATCATGAGCAAGCTTGGACGAGACAACAAGGGAAAATTACACTCTAAACATACATACATGCATACAAACAATCCAAGAGCCTGCTTTAACGAATAGACATCGATACTCGGATTAATGAGTCATTTCACAGAAGCTCTTGCCAATATGTCTTAACAATCTCATTCCAATCATGTGACGAATAAATAGTGTTCTACGAGATTAAGCTAGCATATATTGAAAGTTCTAAATGGCAAGCAAAGCAGCGACAGTGTTAACAAACCACAAAGACAAGCTCAACCGACCATGTATATTCACATTTTCGAGTATCTCAACATGTTCGACCACAAACTAAGAAAGGGCCAGCAAGTCGATTAACAAACATCACATGTCATACGGCGAAGGCTCCTCGAAACAAATACAAAGTCATGACTAACTCGTGAAGACAAACAGAGAAGCAATGTCGACGAACCATCACATTGGGACAGAATCACAACGTAAGACATATTCCAGACTTAAACAACCAAACGAAATATGATACCTCATGTTGACAGCACCACAAACCACGACTCAACTCCATAAAGAAAATTTAAAATAATAAAGAAAAATGGGACATGTACCTTTCTCGGAGCAGCGGAACAGGGATGACGGCGAGCAGACGACGATGACTTCCCCACGCGACCCACGGCTAGACGTTTATCTCGTTTGTTCTTCGTATTGGAGTCCTATTCCCGAAAAATCCCGACTGTTTTGCTACACGAACAGGACAGGAAGAAATCTTCTTCAAAGTCTTCCTAAACCAGAATTTCTAACCAAATTTTCTAACTTCTTTTGGTTATCTCAGAAACAATGGAATAAACCCAGAATTTCAAACAACGTTCCCAACCCAAAATTTCCTAACAAGAGACGGAAGAAGCAGAGTCTTTTTTTTTCGAAAATTTTCTCAACCAAAATCCCTAACCCTTTTCTGTGTCTTTCTTTTCTTTTTCAAAAATCTCAAATATCTAACATTCTTTCAACTCCCAAAATTCCCAGCGCTCCAAGTTCTGCTTTTCCGAATTTTTCGATGAAAAAATCTCTCACCCCTTTTCTGATGAAGAAAAGGGGTATTTATAGAGGAGAAGAAATAGCCCCCCTTTTCTCCTTTTGAAATCGAAACTAAATCCCGTTTCGGTCAAACTTCACATGGCACAGGCGTGTTGGAGAGGATGAAATTGACACCTCGCAGACCTACTCGACAGCGTATGGGTCTCTGCGCTTCCGTTTGGTTCACGCGAAGAGAAAGATGGAAGGAGAAGAGCGTGGGGTTGTTGGAGTCTCTCTGTTTTTCGCGTGATTTCTGGGGAATTATGGGTTTTTGTCGAGAGGGGAAGGAGAGAGCGAGCGTGGGGGAGACGCGGGGAGAGGATAAGAGGAAGGGAGAGAGCGAGGAGAGAGCGTGGGGAGGGGGTCGCGTGGCTGCGCATCTGGGAATTGTTTGGGAATTTTGGCTTTCTGTTTTGCTGGGTTCGCGTGGGGTGAGGGGAGAAGAAGGAAAGGGGAACGGGTCGGGTCGGGTAGGATGGGTTCTGGGTCGGGTAGGATGGGTTATGGTGAATTGGGTTGGGCTGGAGAATAAAAGAATTGGGTGTTGGGATTAATTTGTTGGGCTTGTTTTAAAAATGTGTGTTGGGTTGGTATTTTTTAAAAGAAATAAAAGGGGATGTTGGACTGGGTTGAAGAGGATGGGCGTCTGGGGGGAATTGGGCCTTGAGTCTGTGGGCTGTTGGGAATATTGGGTGTAAGGAATTTAGGCAAAATGGGTTGAGGACGAAAGTGGGCCTCGGAATAAGAGTGGGCTGATGGGTAAAGGAATGGGTTCAAGATATACATATATACATATACATATATGTATATACATATATGTATATATGTACATATATTTATTTTCGCAAGGTTTATAAAACTAACTAACTCATACCGACGCGGGTCAAAATTGGGTGTCAACAACTGTCCCTCATTTTACTTGGGTGGATGCAAGCAACTCGAGGAAAATGAAGTTGACCGAACCAATTTTGACCAACTCCACTCGCCTTCAGGAAGAAGGATTTGTCAAAGAGTTTAGGAATTATGGCCGAACCCTTGCAAACGGGTCTCCTACATACCTCAGGCTATATGAGAATTCAGGCCACTTGTAGTTCGATAAGCTTGATTTACCGCACGATTTCAAAGAATCAAAAGCCACCTTGACACCGAATTATGAAGGTATCGAAATGCTCGAGAATAGAATTCGAGAGCGAATGTTGGAATACAGCTGAGTTTTCAACATTGAATCCGCCTACATACCCTTAAACCTTCGGAATCA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 38
GEN 3040
GGCTGTGTGTAGTTCGACTCGATCGTTGGAAAAGGAAAACTATTATGCTAGCTAACCTTCGGTTTTGGACGAGTGACACATTAAACGAGTATGAAAAAGAGGCTTGTAACCTCTTAGCCATGAATGTGGCGCGCTTCACATCCATAATTAAGAACTTACACGGACATAATTTCCAAATCTTTTGGCGTATTGTCACTCAGATTGGAAACTTGCTTCCGATAAAACAAAACTTTCGGAAGCGAGACAGAAATTGACAGGACTAGAGAAAACTCGTATGCCTCGAGAGGGTGTTTCGATTGTGGTGGAAGTCGCTCCTCTGCTCGCGTCCTAAGAGTTTGACACTCCTCTTTGGACTGTGTCCCAGTTCGCTGCTAAGAAAAAGTTCCACGTTGTGCTGCATCCTTTTTGATGTCGTCCGCACCTGTGGTTTTTGGAGAATTCATCCTTTTGGATGCTCTCGACAAAGACTGAGATAAAACTCGTCAGCTTAAAAATGCAATTAGGATGGGAGACAGTGTCTTTTACCATGTCGACACTTCATTGTTCTCCTCCATGTTCGACGTCGACTCGATCGGTCTGACGTCCAGCAAATAAAGCTTATGCCTTGTGTTTTGCAATATCATCTTCAGTGTATTCCATACTTGCTGTCAAAATAAATAAATAAATGCTGATGCGATATTGATGTTTCTTTTGTTGTCATCTTCGATGCTCTCCATGATGTTTATTTTTATAAGAAATAACATAATGCAGTCGAGGCCAATGGATAAGTATTGCTCTTTCTTTATCCAAGTACGTCTTCTTGCGGGTCATGAATATCTTCCCGCGATACGTAAATTCTCGCGAGATATGAATCTTCTCGCGATGCATAACTTTCAGCGAAGAATAAAATCTTCTTGCGATGTGCAAACTTTGACGAGGCATGAATTCTTCTCGTCGTGCATAAATTTTGACGAGGCATGAAATCTTCTCGTCGTGCATAATTATTGACTCGCGATGCATGATTTCCGCGATGCATGATTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTTGACGAGGCATGAAATCTTCTCGTCATGCATGATTATTGACTCGCGATGCATGATTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTTGACGAGGCATGAAATCTTCTCGTCATGCATGATTATTGACTCGCGATGCATGATTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTTGACGAGGCGTGAAATCTTCTCGTCATGCATGATTATTGACTCGCGATGCATGATTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTTGACGAGGCATGAAATCTTCTCGTCATGCATGATTATTGACTCGCGATGCATGATTTCCGCGATGCATGATTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTTGACGAGGCATGAAATCTTCTCGTCATGCATGATTATTGACTCGCGATGCATGATTCCCGCGATGCATGATTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTCGACGAGGCATGAAATCTTCTCGTCATGCATGATTATTGACTCGCGATGCATGATTTTCGCGATGCATGACTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTCGACGAGGCATGAAATCTTCTCGTCATGCATGATTATTTATATTGCCCCCGTGAATAATAACGGCAAGACGACCTCCAAATAATATATCGACTTGATCGATAATGATAAAAATAATAATTGCATAGCTGTCTCTTCTGAGGTAATGCATTGTCTTGATCGACAATAATCATCTTGCTTTGATAACGCGATGCAAATAGCGTCTTCTATAGAAATCGTGAAATCTTCGCTAAGATTCCTTTTTGATCCACCTTTGAATCTGGCCAGGCACTTTGTAAATTTCATGTATTGGGGAACGGCTTGAAATAAACAAACACATTCACAAGCATCTTGGCATAAAGATATGAAATGCTTCTTGCTTTCAATAAAATCACCGGCTTTGGAAATAGTTTTCGCCTTCTCCGTATTCATCAGTCGGAAGAATTCGGCCGATTTCGAAGTGAAGCTATAAACCTGCATCCACAGAAAAATTGTCAGTTTTAAACATACTGATCTGTGTCGATCCCTTCGATTGTTTGCTCCTTGTCTTGACGTCCTCGGTTGATTTCCCGATTTCTCGACTCTTGATAGATAAGAAAATATCTTATGCCAAAACAGATGAAATATAAAAGGGAAATTTTTAAGAGAAGCAAGAAATTTTTTAAGAAATAGTATCTCGAAAAAGTCAATGCCAAGTCATGTCATGTCAGGCTTAACGAGCTTCTTTGAGTCCGACGCTTGTCGAATGAATTTCAGAGGCATTCCAGACTTGTGGGGAAGTCCCATGACGAAATTTTGAGGCCATCCTCAAAATTTCTGTCCCAGTTTAGTATCTTGTTCATCTTTCCATTGTAACCGAACAGATCTCGGAATTTTTGAGATCTTCTAAAAAATTCTGTCCCAGTCGCAAATTCGAAATGTCTTTAGTTTGATCCGCTGAAGAGAAGGTTCCTTCTGGAGAATTTTTGAGTCCCTCTCAAAATTCTGTCCCAGTTTCTATTGTAGAAAGGGGGGAATAGAATTTACGAGATATATGACCGAGCCCTTGTGGCGCCTACGTATCCCGTTGAGGCAGGAATCAGGTCAAACGTAGTTCCCCTTTAAAGGTTAACAAGAATAAAATTTACAAAGAAACCGACCGAAGCCGACAAAGGCCGCCTACGTATCTCATTCTTGAGAATTCAGGTCGAACGTAGTTCAGGTCGTAGAATTTGGTCTTCTGTTTTGACTTGTTAAAGAAGTCTTACTTCAAATGAAATTTTGAGATCCTCTCAAAAATTCTGTCCCAGTTTCCATTATGGAGAGAAATGGAAATCTTCTGGGTATATGACCGAGCCGTTGTGGCGCCTACGTATCCCGTTGAGGCAGGAATCAGGTCAAACGTAGTTCTAACGTAAGGAATAATCAAGGGGAATAAATGGGGTGACCGAAGCCGACATAGGCCGCCTACGTATCTCATTCTCGAGAATTCAGGTCAGACGTAGTTCGTTACAAGAGGGATAAGAATTCAAATTCGAACCAATACAAGCAAACAGAAGATTACAAGTAAATGGTGGGAAATGCAAAACGAACTTCACACGTAATATCTCTTGACAGCATCTGAGTTGATAGGTTTGGGCCATACAGCGCCATCCATCTCTGACAAGACTAAAGCACCTCCAGATAGTACTTTGCGGACCATGTAAGGACCTTGCCAGTTTGGTGCGAACTTTCCTTTGTACTCGTCTTGATGAGGAAAAATACGCTTAAGAACCAACTGACCAACTTTAAAATTTCTGGCTCTTACTCTTTTGTGAAAAGCGCGAGTCATTCTTTGTCTATACAACTGGCCATGGCAAACGGCAACCATCCTCTTTTCATCAATCAAGGCTAGTTGATCAATCCGTTTGCTAACCCACTCAGCATTACTTAGCTCAGCTTCTTGGATGATCCTCAGTGACGGTATCTCGACTTCGACAGGTACGACTGCTTCTGTTCCGTATACTAGCAAGTATGGAGTAGCCCCAGTTGATGTTCTGACCGTCGTTCGATAACCCAGTAGAGCATATGGCAACATTTCATGCCAACCCCGCTGCTTGTCAATCATTTTCCCCAGAATCTTCTTGATGTTCTTGTTGGCGGCCTCTACAGCTCCGTTCATTTGAGGGCGATAAGCAGTTGACCTTCGGTGAATAATCTTAAATTGTTCACATATCTCTTTCATCAGATGACTGTTGAGATTTGCACCGTTAT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 39
GEN 3040
CAGTGATGATGGATTCTGGAACTCCAAATCGGCAGATCAGATTGTTGCGGACAAAGTCGGCCACCACTTTCTTGGTTACCGATTTGTAAGAGGCTGCTTCCACCCATTTGGTGAAATAATCGATGGCAACCAAAATAAATCTGTGTCCATTGGAAGCGACCGGTTCTATAGGGCCGATGACATCCATTCCCCAAGCTACAAATGGCCAAGGTGAGCTCATAGCATTGAGTTCGTGAGGTGGCACCCTTATCAAATCTCCGTGCACTTGACATTTATGGCATTTTTGCACGAATTTGCAACAGTCGTTCTCCATAGTCATCCAGAAGTAACCGGCTCGAAGGACTTTTCTTGCCAAGGTAAGCCCGTTCATATGTGTGCCACAGACTCCAGCATGTATCTGTTCAATAAGCCTCACGGCTTCAGCAGCATCCACGCATCTCAAAAGACCCAAATCTGGAGTCCTCCTGTAAAGGACTTCTCCATTCAAAAAGAAATTGAGAGCCATACGACGTATCGACTTCTTTTGATTAGATGTGGCGTCTTCTGGATATGTCCCAGACTCCAAGTACCTCTTTATGTCGAAATACCAAGGCAAACCATCTGGTTCTGATTCCACGTGTGAACAATGGACTGGATGCTCCTTCAAATCTATATCCAGCGGGTCGATGTAATCAATATCCGGATGTTTGATCATTGAAGCGATGGTGGCAAGAGCATCAGCTAATTCATTCTGTATTCGGGGAGTATGTCTGAACTCGATCTTGCGAAACCTTTTACACAGGTTTTGCACATATTGTACATAAGGTACAATCTTTGGGTTCTTCACAGCCCATTCTCCTTGAACTTGATGGATCAAGAGGTCTGAATCTCCAATAACCAGTAACTCGTAAACATTCATGTCAACGGCCATTTTCAAACCGAGAATGCAAGCTTCATACTCAGCCATGTTGTTTGTGCAGTTGAACCGTAGCTTAGCCGCCATAGGATAGTGCTGACCAGATTCTGATACCAAGACTGCTCCAACACCTTTACCCTGGTGATTCACCGCTCCATCAAAGAATAATCTCCAACCTGGATAAACTTCAGAGATATCTTCACCCACAAATGACACTTCTTCATCGTGAAAATATGTCTTGAGAGGTTCATACTCTTCATCAACGGGATTTTCCGCAAGATGATCAGCCAAAGCTTGTGCTTTTATTGCCTTCTGAGTCACGTACACGATGTCAAACTCACTCAACAGCATTTGCCATTTAGCCAGCTTCCCGGTCGGCATCGCTTTCCGGAAAATATACTTCAATGGATCCATTCTGGAGATAAGATATGTAGTATACGACGACAAATAGTGCCTCAGCTTCTGGGCAAGCCATGTCAGAGCACAGCATGTTCTTTCCAGCAAAGTGTAACGAGACTCGTACGGAGTAAACTTCTTGCTGATGTAGTAGATAGCTTTTTCCTTCTTCCCTGTCTCGTCGTGTTGACCAAGTACACATCCAAAGACGTTATCCGAGACAGACAAATATAGCAACAAAGGACTCCCTTCTCGCGGAGGAACCAATACTGGTGGGTTAGACAAGTAGCTCTTGATAGCGTCGAAAGCGGTCTGGCACTCCTCAGTCCACTTAGTTGGGGCATCTTTCTTCAGCAACTTGAAGATAGGCTCACATACCACCGTCGATTGTGCTATAAAACGGCTGATATAGTTTAACCTCCCTAAGAAGCTCATCACCTCTTTTCTCGTCTTCGGCGGAGGTAACTCCTGAATTGCTTTGATCTTGGAAGGGTCGAGCTCAATACCTCTTCTGCTGACTATAAACCCTAACAATTTCCCAGCTGGGACTCCAAAAGCACACTTGGCGGGATTTAGCTTCAAGTTGTACCTACGCAAACGTTCAAAGAATTTTCGCAGGTGTGTCAAATGATCCGAACTCTCGCGGGATTTAATTATGACGTCGTCCACGTACACTTCAATTTCCTTGTGCATCATGTCGTGGAAGATAGTCGTCATGGCTCTCATGTAAGTGGCACCGGCGTTTTTGAGCCCAAACGGCATCACCCTGTAGTGATATACCCCCCAAGGTGTGATGAAGGCCGTTTTCTCCGCGTCTTCTTCATCCATCAGAATCTGGTGATAACCCGCGTAACAATCCACAAATGACTGCATCTCATGTTTCGCACAGTTATCAATCAGAATATGGATATTTGGCAACGGGAAATTATCCTTTGGACTAGCCTTGTTGAGATCTCTGTAATCAACGCAAATCCTGATTTTTCCATCTTTCTTGGCGACCGGAACGACATTCGCCAACCAGGTGGGATATTGCGTTACTTCTACCAACCGGGACTCTATCTGCTTGGTGATTTCCTCCTTAATCTTCAAACTCAACTCAGGCTTGAATTTCCGAGTCTTTTGCTTCACTGGCTCGAACCCTGGGTTGATAGGCAGCTTATGAGATACGACATCGGTACTCAGCCCCTGCATGTCACTGACCTTCCAAACAAACACATCGCTGTATTCGGCAAGCAAATGAATCAGGCTCTCCTTCTGAGTTTCATTCAGGTGAGTGCTGATCTTAACCTCTTTGACACATTCTGAATCTCCCAAATTTACCGTCTCTGTTTCCTCCAGATTCGGTTTATGTTGATTCTCGAACTGTCGAAACTCTTCTACTACATAGTCTGGTTCCCCACTTTCTTCATCGTAGTCGTCAGCCTCATCGTCATTTACCTCATTTTGTTCGTTTAGCTCATGACATGACATGACGTTGGCAGGTTTGTAACTTACGTTACTGAAATCATAAACAGACAAAATTAGAAAAGTAAAACATAACAAGCAATCGAATAAACAAAGTCAAAATAAGGAGGCTTTCATTTAAATTGAATCAAGATGCAAAGTTGGGTCATGGCACAAGGCCACGTCGCCCTTTAAACAATCATAACAAAATTCAAAATCAAGAAAATGCAGAAATGGTGGGTCTCGGGCCTCTTCCGAACGACCACCGATTTTGGCATGCACAAAATAATTCCTTTCTACCCAAAAGTCCGGGACATCAGGATTGGTGTGGACGTCCAATTCTTCAGCATCTCCCCCGGTTCAGCATCGCGAAAGCCAGCTGGCTCGGCCTCCTCCTCTATGACGGCATTGATCTCCTTGAACAGGTCACAGATTCCTTCCCCGTCGTCTTCAGGCTCGGCATGCTCCCGAATTGGAAAGGAGTGATAGAGATGCGGGATCGGCTTAGTCAACTCTTGATCCCTTCTCCTCTTCATCTTCATATCATCATCTGTGGGGATGTACCCCAAACCATACTTTGATCCCTGAGCAAGGACCGGAACAGGCTCAATAATTCCTTGGGAATCTCTTCCCAATCCGAAACCTGGCTCGAACCCATTTTGCAACATCACCGTGGCTATCATTTTGTACACGGTTGGCATGGAGTTGAGGGCCAAATCTTCGTTGGTGGCATTCACCAGCTCCACCGTGTAAAAGTCTGTGCCTTGCGGCATCTCATCAATGACCGACACCTGCTTGCCCGAGCGACTTCCCTCGCCGTGAATCACTAACTCTTCATTTTTCCACACAAGCTTCATCATCTGATGGAGAGTAGAGGGGACGGCTCCAGCCATGTGGATAAACGGCCTTCCCAAAAGAAGGTTGTAGCTAGTGTCTATGTCCAATACTTGGAATTGCGCACTGAACTCTGCGGGGCCCATTTGAAGGGTCAAAGTCACAGCCCCCAAGGTGTCTCTTTGCACACCATCAAATGCCCTTACATTGACCTGATTCTGCTCCAGTTTCCCAAGGTCAAAATTTAGTTGCCTCAACGTCGTCAATGGGCAAATATTCAAACCGGACCCATCATCTACCAAAACACAGTTGACAACCTTTCCACGACATATCACGGTAATGTGTAGCGCTTTGTTGTGGGATCTTCCTTCGACTGGCAACTCATCGTCACAAAAGCTGATCCGGTGCCCCCGAATGACTTG
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 40
GEN 3040
ATGAATCATAGAAGCCACGTTATCACTACTTGTACCTGAGGGTACGTATGTATCATCAAGAGCTTTCATTAAGGCCTGCCTGTGGGACTGAGAGCTCATCAGCAAGGCCCACACGGAGATCTGAGCCGGAGTCTTCTCTAAATGTTTGACGATGGAATAGTCCTTCGGTTGCATCCTTCTCCAGAATTCTTCTGCTTCCCCCTCGCTTATCGGCCTCTTAGCATGGTCCTTCTTCTGTCCTCCGAAGAAACCTCATCAGGAGTGTAGCACCTTCCGGACCTGGTCATGCCTTGGGCCACGGCAGTTTCAATGACAAATTTGGGCTTAACGAGGGTTTTCGAAGGCACCAAGGCAACAGCCTTTGCAGGTGTCAGAATAACAAACTTCCTCCTCTCTTTGACACTTAAAGAAGCGACAGCCCTTTCCAAGTCCTCATTCATAATAGGGGTAATCATCTTTGTTCCACACCCGTCTTCATCCGTTTCAATCATATTAAGGTTGTCACCTCCATGATTCGGCAACGGATTTGTGTTGACGTTTGGCACCGCCGGTTGAAGAGAAACTACCTCCTGATCGATCAGGTCTTGGATTTTGTGCTTAAGGTTGATGCAATCTTCCGTGTCATGTCCAACACGATTGGAATGATAAGCACACCTTTGATCTGGCCTGTAGAACTTTGAGTTGACATCCCCGGGTTTGGGCCCCACAGGGTGGATGTATCCATCTGCGGCTAGCCTCTCAAATAGTTTGGTCCGGCTTTCAGCAAGTATGGTAAAGTTCCTTGAAGGCCTTTTTTCAGATCTCGGACGGGAAGTATCATAATTGCTTTGCCGAGGGGGAGGCACCCGCTGATAATTTTGGGTATTTGTACGAGGGGCTTGGCTCCGGGGATAAGGGTTTGCCTGGAAATTTGGCATTGGAGCTTGGTAATTCGGGAGGGGGTCCTGGTATAATGGAGCTTGGACTTGATAAGTGGGCGGAGGTGGTCGGTAGCTCGACTGTACGTTGGCACAGTTGGGAGCAATATTCTGATAAGGGGATGGGATCTGGTATGGTTGAGGGTAATTTGGGAATATGGGAGAAAAATTTTGGAGATTAGAGGGTGGACCTTAGTACGACGAAATTTGAGCATTCTGATAGGTGGGGATAGTATTGTGGTTATTAGGATGATTGGATTGTGGGTAGTAGGATCGGTGAGACTTTGGGGAAGGCCTGGAACGATCTTGGGAATGTGACGGGTTTTTAGGGGTTTTTCTTCCCCCATACGAGACAGCGGCAACTTCCTCTCTTCGCTTTCTTATCAATCCTGAAGACCCAGGCGACGCAGATACACGGGCTATCTTCCCCGATTTTAGACCATCTTTGATAGTCTCACCGACTTTGACTATCTCAGCGAATTTAGCTCCGACCAGCAACATGATTCTATCATAGTACTCAGGCTCCTGTACCCGCACGAACACTTCCACAATCTCTTTCTCGGTCATGGGTGGCCTTACCCTCGCCGCTTCTTTCCTCCACCTATAGGCAAATTCCCTATAGCTTTCAGTTGGTTTCTGCTTCATCTTCTCTAGAGAATACCGATCGGGCACTATTTCAACATTGTAGGCGAATCGGTCAATGAAGTCCTTAGCCAATGCATTCCAACTGGGCCACTGCCTGGTTTCATGTGAAGTAAACCACTCGAGGGCCTCTCCACACAGACTTCGGCTGAAAAGCCGCATCAACAAGGCCTCATCCCTGCCAACTCCCACGAGCTGGTCACAATACGCTCTCAAATGCGCCATAGGATTGCCCACTCCTCCGAAGGTGTCAAACTTCGGAATTTTAAAACCTTCGGGAAGGTTCAAATCTGGATGGATGCACAATTCTGCGTAGCTAAGTCCGACGGCGTCTGGGATGCACTGCAGCTCTTTCATAGCCCTTTTGATTTCTTCCTTTATATCGATCTTCACTTCTTCTTTCGCCTTCCATTCTCTTTCCTGTTCTTCATAGTGATCCAACTCAGAACCGTGCACCTCGTGCTCGGCAGGAACGGGAACTTGGAAGGTGGCTCTTTTTGGTAGGGGTGGAGCTATAGAGGGACTTGGAACGTTTTGGACGGTCTGGTAGTTTTGTGGGTAGGCCTGAGGATTGGTGTTCTGACTCAAATGCGGATGAAATGCTTGGGTATTGAAGGCAGTTTGAGTTTGGTTTTGATTTTGTGGCGGTGGGGCAGTTTGAGTATTCTGAGGATGGGGTGGTATTTGAGGGTGGTTATTTGGAAGAGGTGAAGGAGTTTGGTAAGATGCAGAAGCGTATTGAGGGTTTTGGGTTGTGAGGTCAATCACAGACGGATTATGCACAGGTGTAGAAGGCTGGTTCTGAGTTGGATCTATGTTTGATGAAAGGAAGTAGACCGGAGGTCTTGCATCAGCAATATTAGTACCAAATCCAGGTGGAGGCGCATCCTGTCTCCGTTGCATTTCAACTTTCATTTCAGCAATCTGTTGCATCAGTTGCATGATCAATTCGTTCTGTTCCACTACAGTGGGCTGAGCCACCACAACGTCTGTAAGACGCCCTTCGTCATTGTTGTCTCCCATAACTATTTTTCCTTTGTCTGAATTTGATCGAGGGAAGGAATCTGTAGGACCTTTTGACCTGGTGAAGTAAGAATGATCTGCCAGCTTTATCGACACAGATCAGTAAAAGGTACCTGAGAGGTAGAAACAACTCAAAGGCAAATTTGTTAGTGCATATCCAGAGTACAAAATGCATAATATCGCACATAAAACAGATAAGCACACAAGTCGTTTTGTTTGAGGATATCTTAACCCACGAATAAGGACGGATATATATTTTGAACAAACAGGAATTTGTTTGTTTGGAGATATCTTGGGAAATCTGAATCACGTTGAGCTATGGTCTTCTTGCAGCTGCTTTGCGACGTCTGTTGATCTTATCTTCGTATCTCCGAAACATGGAGGAGAATGAAAATTTTCTGTGATAGGGGCCGGGCCGGGAAGGCTGCCTACGTATCTCACGGGGAGAATTCAGGCCCAACGTAGTTCGGATTTTAGGATGAAAATTTTCCATTCATTCTTTCGTTGTGATTACAAGGGAATTGAAAAACAACATTGAGATTTCTAGAAGACCACATTTGGAGATTTCTTGTCTTGTGGCTGCGCCATTCCTTTCCTTTCATGCAGTCGAAAATGGAGGCTTGTAGACGATTTCTTCTTCATCATCCTCCTCAATCACTTCATGGCCGGGGCCACTGTTGCCTGTTCCCTGATCACGGGCGAGGTATTTTCCGCCCTTTGGGTCGCTATGGGTTGCCAATTCCTCGTCGAGCGCCGTGCTTCTGTCCAACAACACAGCAGTCTCGATATCTATCTTCGCCTCTCTCGCCTTTCCTTCGTGTATCTCCAAACGAAGCAAGTTCAACCTTTTCCTTAGGCTTTCGGTTTCCATCTCAACCTCCTTCTTTCTCTTTATCTGTGACGCCAGATCTTCATCCAATGTTGTGACCGCAGCTTTGTAGATCGCCGTGGTCGCGTCTACTTTGAGTCCTTCTTCCTTAACCTTCTGAATCTCTCGAGTGACTCGCTCGATCTTTCTTTGCAATTCCTCCTCAGTGAGCTCCGTCTGGACGTTCTTGCCTTTGTCCATAGCGGTGATTCGACTTTCCTGAGATGATAGAGCGGTATTTAGGATTTATGGTATGGATTTTGTGTGAGAAACTTGGGACTCGGGAATTTTGGTCGGACATTTGTAATGTTGGCTTCTGTATACTCTTTTGAATTTTGGCTAGGAGTGGGTTTACGATATCCTCAATCAAAGCAACATAACATATAAACATTTAAACAAATATATCGTGTCCTAAACATGCATGTGACCCTTTTGTGCCAAGGGTAGGCCTAGCGATCTGAAGGATGTATTGCGCCATTTAAATAAGTAATTGTATCCCCGAAACAAATTTCACTAGATGTCATAGCGTTCATTACACAA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 41
GEN 3040
TCAAAATTAAACGGGATATATCTTGGGAAGGGGATGTAGTGCAAATACACATATAAAATCAATCCCACGCAGGGGAGTTAAAAGGTCGATGATCCAGCTCCTCTTCTGGCCTTCTCCACTTCTTCTCGGATAATGCATTTGCTGGTGAACACGTATGGCTCCGCGAACGCAGCTTTGGAAGATGTTGGTACGGCTTGTAAGGTGTTCATCTGCTCGTCCAAAATAGTGTCCAAACGCATTAGACAAGTCCTTGTGTCAGCTAATTCTTGTGACATTACGGCTAAAACTCTCTGATTTTCTAATTCCCGGAGTTGGTACGCTTGCTCCCTTTGTTGAAATTCCTGTTCTTTCTTATAGAATTCCTGCTCCCTCCGGTCCCACTCTTGCTGAAAATGGTACGCGTGAATCTGAAGCCTTGTCTCCACGTCTTCGATTTTCCGACTTGTGCGTGGTGTCGGATCCACTACACCATGCAAATTATTATGCAACCATGTCTTATATTCGTCGACATACCCAGCTCCATACCTATCTTCGGTGACCTCCCACTTCATGTTGACACGTCCGGCCCATTCCTGGAGGATAATTTTAGCGAAAGGTATTTTGTCCTTCCCGTTGTAATCAACAATGAAAGAGCTGGCATCTCCTTGGGCGGGTATGATTTGGACTTTCCCAAATTGCCGCATTACTCGTGTCGGGTTGTAAGGACGCAATCCTCTGATGCCGGGAAGGACTAAAAATGGCAATTTGGCCCCTTGGGCTGCTATCATCTGAGACCTGAAGCTCGGTAATACCCATTGTATGTCTCCTTCTTTCAAATTGTAAAACACCTTTGCCCACGCATCCCTGGTGGTGTGAATTGGAAATTTACGGATACTCAAGTAGAACTCGTAATTATTGAGAGGACTTGTTTGACTTGGTTCGGCCAAACCATGTAGCTGGGGGTTGTCTCTTTTGCATAGATGCTCCGTCAACCACCATTGAAGTAAGATGTTGCAACCTTGGAAAAACGGAAAACCATTCTTGCACAGGCTCAAAGATCGGTAAATGTCGGACAGGATGACTGGGGCCAAACAGCAATATTTCTTTTCTTCAGATTTTCTACCGACTCCTCTGAACATGGCATTCACTACCATTATTACCCGGGTGTCGATCTCTTTTCCTTCGTCTTTAGGGAACACCATAGTTCCCAAGAGGCATGCGGAGAAAGCGATAGCTTGAGTTTGCCTCCAAGTACTGTGATCATGGAATTCGTCCGGGAACAATCTAAAATAATTGTCGTGCCCCCACCTCTCGAAAAACCTCATCAGTGGAATGTTGTGAGTCTCTAGCCAATTCGCATTCACTAGGGATAATATGTCTCTTAGCTCAGTCTCGGTTGGCCTATCTGGCAGGAGAATGTGATGATCGGGACGCTTTTTCCTTTTGCCACACGTCCCAATCGAGTCAAGGCAATCTTTGATCTCTTCTAATGTGGGTTTTATTTCGACATCTCCAAATCGAAAGACCATCTTCTTATCGTCCCAGAATCGGGTGATGACTTCGATAAACGTGGGCCATACCTTGAAGTCCATTATCGAAGGTAGGTGACCCAAGTAAATCGAGATCTCTCTCTTCTGACACTCCTTAAGATTTTCCCACCATACTCGAAGAATGTCGGGTGCCTGGGTGACCATTTTGAAGTGAAGGAACTGACTTGACATCTACAAAAACATACAACTAGTTAATTTTCAATCCCCCCGATACAACAACTCGTTAAACACGCAATACATCCTTCAAATTTAAACAACAAACATGAGCGTCCTATCGAGCCGCAGGCTCTTTGGACTCAAGGTGGACTTTTCTAATGGGCCCGACACTCCTTGGGTGTCGGGTCATCTTAGGCCAATGCGCTATTTTCGGGATTTGGTTTCGCTCTATCCTAGTTTGACTAAGAGTGGGTTGTATTTAGAGTGGAATGGGTAACCCAAGCGGACTACTTGGGCTGGAACAATTAACGATCGTTGACTATCAAGTAATCAACTACACTCGTCAGGGTGCCCCGAGAAGATTTTTGGTTAACGAACGACTGCGAACCCGCATAATCGTCCTTTAGTGGAAATGTAAAAAAGTGTCGTTTGACGGAAGTATGTATGCTATGTATGCAGCAGTTTAAAAATCTAAACAATTTAAACATTTAAACAGTTAATCACAAATAATCAAATACGTTAATCTTAAGGTTAGAATCCTCCAAGTCCCCAGTGGAGTCGCCATTTCTGTTTTATCGAAAAAGGGTGGTGATTTTCGAAAAGAGTTTGTTTTTAGACTTTTAGGAGTCGCCACTTAATTTTTAAGAAAAATCAAGAAAACTTCGTTTCTAAACAACTTAAACAGAAAAAATTGTTTTGAAACATTTTAAGGGTTCGGGATTCCTATTAGTATCTTAGGAAGGTGTAAGGCACCCAAAACACTCCGTTAACTTACGGTTTTCCAATGATTAACTATTTGATTATTTTTATTTTTAACCTTTGCAAATTTATTTGAACTTTTAACATGACTTACTTAGCCAAACTTAGCCAATTTTGAAATATTTATTGTTTATCTTGCCAGTTTACGAAACCATTTCAAAGTTAAGCTTACTCAAGGTTTTATCTAAACAAGCCTTACGAATTTGAAACAATTTATCATTTAGAAGTTAGTTTTAAAATTTTTTAGGTTTGATAAAATAACAGGCGTTCGATGAGGGTTTGGAGTTTCCTAATCCTAAGCTAACGGCATTGAAAACAAAATAAGATAAGCAAATAGTAAAGAGAAGGATAGAGAGAGAGAGATTTGGGTCCAACTCCGGGTTCTGTTATCCTTGACCGAATGTTGGACCCACCTGCCTTTGGGTTTTTTAGCCCATTTCTGTACCTGTCCTAATCTAAACATACAAACATAATATAGAAAACAAAAAAACGACAAGAGGGCTTAGGCCCATTACAAAATATGCAAAGTAAAATAAAACGAAAGAAAAGTCTTTTAGTCCATCGTCTTCAATCTCCATGACCCTTGTCTTTCTACACTTGCTCGTTTTGATGAATTGACTCGATAAAGTTCAGAGTCTTCAAGACTCTCTCGAAATGCGAGGAGGATAATAAGATTCCCATAATGGACTCACGGGGAGTTTTCACATGTCACAAAAAAAACGATAAAATGAAGATTGGCATTTTTAAAACTACAATTAGAATAAGCAAGGTTCTAAAGCATAACAAGGATATCTTTATAATGCAATACTAATTAAATCTTCATGAGAAGCAAAGCCAACATCCAACAACCTTAATGGTACTTAGAGATATGCAAATCAGTCAACCTAAATGACACTACATAACGATACATAAACTGTGCGGCGAAACCCCAAGTGTAGTGAAACCTTCACACAAAATTGTTGGTAATTTCCAAATTTTATATTTAAAGAAAGGACTTAGCATGGGATAAATAACGAATACAAATAGAGATGCATCATAAATTGAGTATCAATTAGCCGTTTTGCGCTTCAAACATTTGAAACAAACTTGAACCAATCATAAGAGGACAGCCTCATCAAATAAAACAGGATCCCGATCACCTTATCACCCAAACATCTAACACACTAAGCGAAAGGACCATTCAATCGACCTACTACATGGCTGTGACCTCGGACAGCTAACACAAGTGGCCACCAAGATAAAGATGGCCTTTCAAACCACAGATTTCCAAAATCCACACCACCCATACGGCATACAAAACGAAACTTTCATGCAAGACGCCTGACAAACATGATATCTAAACAAGATAACAAGTTTGGGAGATAAATACATTATAGAGAATAAACAACCCTCATATATTCTTAAAGCGATACTCGAACCAAAAGCTTGAAGAAGGTTCGATTGAAAAGAAGCTTACAACTACAACTCCCGCGAGATAGACATCATGAGCAAGCTTGGACGAGACAACAAGGGAAAATTACACTCTAAACATACATACATGCATACA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 42
GEN 3040
AACAATCCAAGAGCCTGCTTTAACGAATAGACATCGATACTCGGATTAATGAGTCATTTCACAGAAGCTCTTGCCAATATGTCTTAACAATCTCATTCCAATCATGTGACGAATAAATAGTGTTCTACGAGATTAAGCTAGCATATATTGAAAGTTCTAAATGGCAAGCAAAGCAGCGACAGTGTTAACAAACCACAAAGACAAGCTCAACCGACCATGTATATTCACATTTTCGAGTATCTCAACATGTTCGACCACAAACTAAGAAAGGGCCAGCAAGTCGATTAACAAACATCACATGTCATACGGCGAAGGCTCCTCGAAACAAATACAAAGTCATGACTAACTCGTGAAGACAAACAGAGAAGCAATGTCGACGAACCATCACATTGGGACAGAATCACAACGTAAGACATATTCCAGACTTAAACAACCAAACGAAATATGATACCTCATGTTGACAGCACCACAAACCACGACTCAACTCCATAAAGAAAATTTAAAATAATAAAGAAAAATGGGACATGTACCTTTCTCGGAGCAGCGGAACAGGGATGACGGCGAGCAGACGACGATGACTTCCCCACGCGACCCACGGCTAGACGTTTATCTCGTTTGTTCTTCGTATTGGAGTCCTATTCCCGAAAAATCCCGACTGTTTTGCTACACGAACAGGACAGGAAGAAATCTTCTTCAAAGTCTTCCTAAACCAGAATTTCTAACCAAATTTTCTAACTTCTTTTGGTTATCTCAGAAACAATGGAATAAACCCAGAATTTCAAACAACGTTCCCAACCCAAAATTTCCTAACAAGAGACGGAAGAAGCAGAGTCTTTTTTTTTCGAAAATTTTCTCAACCAAAATCCCTAACCCTTTTCTGTGTCTTTCTTTTCTTTTTCAAAAATCTCAAATATCTAACATTCTTTCAACTCCCAAAATTCCCAGCGCTCCAAGTTCTGCTTTTCCGAATTTTTCGATGAAAAAATCTCTCACCCCTTTTCTGATGAAGAAAAGGGGTATTTATAGAGGAGAAGAAATAGCCCCCCTTTTCTCCTTTTGAAATCGAAACTAAATCCCGTTTCGGTCAAACTTCACATGGCACAGGCGTGTTGGAGAGGATGAAATTGACACCTCGCAGACCTACTCGACAGCGTATGGGTCTCTGCGCTTCCGTTTGGTTCACGCGAAGAGAAAGATGGAAGGAGAAGAGCGTGGGGTTGTTGGAGTCTCTCTGTTTTTCGCGTGATTTCTGGGGAATTATGGGTTTTTGTCGAGAGGGGAAGGAGAGAGCGAGCGTGGGGGAGACGCGGGGAGAGGATAAGAGGAAGGGAGAGAGCGAGGAGAGAGCGTGGGGAGGGGGTCGCGTGGCTGCGCATCTGGGAATTGTTTGGGAATTTTGGCTTTCTGTTTTGCTGGGTTCGCGTGGGGTGAGGGGAGAAGAAGGAAAGGGCAACGGGTCGGGTCGGGTAGGATGGGTTCTGGGTCGGGTAGGATGGGTTATGGTGAATTGGGTTGGGCTGGAGAATAAAAGAATTGGGTGTTGGGATTAATTTGTTGGGCTTGTTTTAAAAATGTGTGTTGGGTTGGTATTTTTTAAAAGAAATAAAAGGGGATGTTGGACTGGGTTGAAGAGGATGGGCGTCTGGGGGGAATTGGGCCTTGAGTCTGTGGGCTGTTGGGAATATTGGGTGTAAGGAATTTAGGCAAAATGGGTTGAGGACGAAAGTGGGCCTCGGAATAAGAGTGGGCTGATGGGTAAAGGAATGGGTTCAAGATATACATATATACATATACATATATGTATATACATATATGTATATATGTACATATATTTATTTTCGCAAGGTTTATAAAACTAACTAACTCATACCGACGCGGGTCAAAATTGGGTGTCAACAACATTTTTAAAGAGTTGTGACTTTTATGAAAAATTGTGACTTTAATAAAGAGTCGCGATTTTTATAAAGGTTGTGACCTTTCTAAAGGAATGCAACTTTTTTAAATAGTTGTAAACTTTTTGATAACACACAAAATACATTTGTTCACACTTCTTTGTTGTCTATAAATAGAGGGATTTTCTTTTATTTTAAAACAACAAAAATTCTTAAACCTCCTCCTCTTCTTCTTCTTTTTGCACAATTAAATAGTTATGTATTTTGCTCATGTTGAGTGACTCACTGAAACTATTTCTTATGTAACAGATCCTAGTATGTAATTTTATAGTCACTAATTTATGCTTATGTTATTAAGGATAAATGATAAGATTTGATAATTTTCATTTTCCTTTACATTATTAATATTTGTTATTACATAATCTTGTATGTAAGATAATATATATAGTGTTTTAGTTTTAATGATCGATAGATTTTTAGTATTATTTTATAAATTAATGAAATACACGTCTTATTTTTCCCTTATTTTCAATATTCCCTTTTATTTCTAAAAACCTCTAAAATTCCTTATTTATTTAATGTAACTGAGTTACATATTAATGTATCTAAATCAATATTTAATATATTTGAGCTGCATATTATGTATTCGGTTTATTTTATAATGTATCTGAGCTAGTTCTTAATGTATTCGCGCTGCATATTAATGCATCCAAAAACTAGTATGTAATGTATCCAAGCTACATATTATGTATCCTATTTATGTTATGATGTACCCGAGCTACATGTTATGTATCCGAGCTACATGTTATGTATCCGATTTATGTTATAATGTATCCGAGATACTCTTTAATGTATCTGAGCTACATATTAATGTATCCGATTTGTGTTACTTTTAAGAGATTAATGTAATTACAAACTAAAGAGGGATAGATTGTAACTTCACATTAAAACTCTGTGATTTCTAAAATTTATCCAAAATCTGTAATATTAAATGCACGAATAGTTTTACTAGTATATATATAGCATATCAAACTATGTTTTCACTTTAAGTTGAAAGGGTTTGTGTATATATATTCCACACGCATATATACAAACTTATTATCAATATTATTTTGGTAATTTTTCATTCATTTTTTTCGCAACCAAAATATTCTTTTTATGGGCAAAAAACTACTTTATAATTAGTTCCTCCAAGACAAGTAAAATATTAGTTTTAGCATCCTTAGGGTAAGCATCATAACACCTTTCCTTAAAAACACTTACACTGTTGGATCACATTTACCGGAATAACAACAATATTGATCGGTCTTAAAAATCGTACAAGGATTGTTATAACATCCTCTGCGAGTTTTTAGCACTCATGGACAAATCATGTTAATATCAACTCTACACCTAGTGCCACGAGCACGCCCATTCGATATAGGGCTAAATTCTATTGACACGTTGAATCTAGCGATGAAATAGACATTCAAAAAATTAAAAAAATATATCATCTTAAAACTTCCACTACATAAATTGTGAAAATTTGTAACTCTTTCAATACAGAATTAAATGGATGGAATGATTACAAGGAAAATTTCTATAAGGGAGAAATTTAATAGGTAGCCACTTATATATTTGATAAGTGTAAGACATCATATTGAAGGAGCAATAGAGAAAATTCATCACATGAAATCATGATATTAATTATGGCTATATTATATTACATACTGTCATTTAATCTAAATTACCAAACTGCAATGATGATTACTTCTTTAATTTCTGGAGATTCAAAGTTAATTAAAAAAACTCTGCTAATGAAATTAAGAAAACAGGACTGTTGGTTCATTGGTTAAGTTTGACTATCGATTTAGCGTGTTATAAATAAAAAATACACAAAAACCTAACTGAACCAATCAATAACCACATGGACAATATCCAATACGAGTTATGAACCTGCCAATTTATTAGCACTAGGCATGTTCAGGGATGGTCGACAGTGTTAAAAAAAGACATGTGTCATAGGCCTTCAAATTTAAATTATAACAGTCGTAAATTATTATTTTTTAAAAAAAGTTTAACATTTCATGAAAAATGAAGAAATCATTAGAAGAATTTT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 43
GEN 3040
GACATATTTGGAATACTATATCTCATGAAAAATAATTAAAAATAAGAAATGATTATACTATCACATGAAAACTAGACGAAATCAATTATATAAAAATTATTAACAATTTAAGTTTAAAACCCCATTTAATTTTTACTTTAGAACGTAATACGCTTGAGCCGCCCCCTCGATTTAACCAGGACGTAAAAGTACTAACGATATTATTGTTTATTCATTATGATAAAACTATATTCAGTTGAAGGGAGACATACATAACACACGTTGGAGATTATTATCACATTAAGGCAACATAGTTTAGCAGACCGTTTAAGATGCTTATTATTTTGGTAATTTTTTCATACCATTCAATTTGTAGCAAAATATGCTTTATTGTGGCATCAATTATGGGCAAAAAACAACCCTATAATTTGTACTACCACTAGGGCAAGTGAATAAGCTAGTAGGATCATCTTGAGGGTAGCTATACGCATTAGGGCACCTTTGTTTGAAAAATCTCGAAAATTTTGTAGGACCACATGGGCCTTGAGTACAACAATATTGTTGTCCTCCGAACGTGGTACAAGGGTTGTTACATCCTCCGGGTACCCTAAGTGGACTAGGGCATTCACCGTTTATATTAGCCGTGCAATGAATTGCATGGCATTTTCCTCCACTAGGATTGGTTGGGGCGAAAGTCATCGGTATATTAAATCCGTCAACCAAAGAAATGTCCCAGAAATCTAGGTTGCTGAATTGGTCCAAAGCATATTCAGCTAGGGTGTTTGGGGGTTTGCCCCACCCGGTACAGTGTAGGACCCCACCACAATCACCAGTTTGGCATGAACCTCTACCAGCACCATTAAAATTGCAATTAGTACGACCCCATATACGTGCCATCTTAGTGCCCCTCGGCGCATTGATGACCCATGTTTGGCCTCGATCGAGACGTCGACCACCGCCTATCGGGGTCGATGCTGCCCAAACGGTGTAGGGACAATTGTTGCGTACCTCGATAGTGGCAGCATAAGTGTAAGTCACTAAAGCAAGGAAGAAGAAAATAAAAGAAGATGTCAAGTAGCCCATGTTTGTGTACATATTTTTGTTTATTTTTGATATATAGTTACATATTATTGGGTAGTTTATATAGGGGTGTGACAACTTTTGGCACCTTGCACATTTAGAATTCCACTATATAAGTTGTGGAAATTTTTACCATAAAGAATTAAATGGATGGAATGATCATATGGAAAATTCCTCTAAAGGAGAAATTTACAAGCCACTTAAATTGGAGGAGGAATAGAGAAAGTCATCACATGAAGTGATATTAATGACAATATTATATAATTGTCTTTTAATTTGTTAGCAACAATAAAAAATTCATAGCTAATTCCTAATCTGCAAAAATGATTAATTCTTTATTTTCTAGAGATTCAAAGTTAATTAATTATAAAACTCTCCCAATGAATTTAGAAAACAGGGAATGTTTTTTGGTTCGTAGGCAAGAAAGTTTTTGGAATTAAACACAACAAAAATAATCCTCATATCCTATTTTTAACTAATACTTTAATGTGTCTAGATTTTTATTAATATGTCTGCTTTATATTAAAATTTCATTTTTTTAAAAAATAATAGGAAAATTCTCAGACCGTCCATAAAGAAAAATCAAAATTAAATAGTTTCTTCTATTCAAAATTAAGATGAGTTGTCACCATACTTTTAGAATTTTTGATTTTTTTATGCAAGAATAAGACTTACGAGAACAAATGGTAATCAAAATAAGATAGGAAAAAGGGTCAAATTTCCTTTTTTTTTCGCCTAACTTATTTTATTAACTAATTCATATTTAGTTGTGTCTAAAACAGGTTTAACTGATAAAGGTTAAATAAAAAAAAATGTTACTACTGTGTTGATTTATCAATTTTGGGTTATTGAATTAACCGTCTTAGAATATTAGTTCAGCTTCCAATTTTAAAATTTTAGTTAATGATTAAATCAATAACCTAATAAAATTATATTAAAACTACTTTTTTTTATTTTTATTTCTATTTATATAATAATGACTTTATTTAAACTTCAAATACAAAACCTATTTTCTTTTGCATTTTATAATCAATGTATTGATGCTGATGTGTTTTGTGCACTTGTTAAAGTTGTGTTATTATATTATTTTAATATTAATATAATAAATGAAGCAGTGTTTTTGCTTGTAAATTTCTAGATTTCTATTTATGAAGTTCTGACTTAAACGTATCTAACGAATAATTAAATAAAAAATCAAGTATTTAAGGATCGATAGTTGATTATCTAAATTATTGATCATTGATTTAGCATGTTTATAAATAAAAAATCGATAAACTAAACCACAAAATCTAACCAGTAAACATTGAGACCATATCGAATACACCTACAAGTAATTCATTAATAAGTTTCAAATTCAATATACAACAACAAAAAAAAAAGGCAAGAAAATGTTGAAGTAAAGTAAATAACGACACACGATTATTAAAGATATTATTATTTGTTCATCAATGATCAAAATATATAAGTTGAAGGGAGTGTGTATACATTACACAAATAAGATATTATTATCTCACCCAACATAAATAGTTTAACAAACCTTTTAAGATACTTATTGTTTTGGTAATTTTTCATACCATATAATTTGTAGCAAAATATGCTTTATATTGTGGCATTAATTATGGGCAAAAAACAACCTTATAATTTGTACTATCACTAGGGCAAGCAAATGTGCTAGTAGCATCATCTTGTGGGTAGCTATAGGCATCAGGGCATCTCTGTTTGAAAAATTTCGACCACTCAGTAGGACTACATGGACCTTGGGTGCAACAATATTGTTGTCCTCCAAACGTGGTACAAGGATTGTTACATCCTCCGGTTACTTTAAGTTGACTAGGGCATTCACCGTTTATATTGGCGGTGCATTGAATTGAGTGGCATTTTCCTCCACTGGAATTGGTTGTGGCGAAAGTCATCGGTATATTAAATCCATCGACTAAAGAAATGTCCCAGAAATCTAGGTTGTTAAACTGGTTCAAGGCGTATTCGGTCAAGGTGTTTGGCGGTTTACCCCACCCGGTACACTGCAAGACTCCACCACAATCACCAGTCTCACAAGAACCTCTACCAGCACCGTCAAAGTTGCATTTAGTACGACCCCATATACGTGCCATCTTAGTGCCCCTCAGTGCATTGATGGTCCAAGTCTGGCCTTGATTGAGACGTTGAAAAATCTTTTTTTTTTCATGTTTCTTGTTTATTTTAAATTTTATATATTTGTTTGTTTGTTTTTTTCTCTGAAAGAACGTTTCTCTCTTCTTCTTTTTTTTATTATTTTATTTTATCATTATTTTTAGTATATTATTATTTTATAGTTTTGTCTTCTTCTTTTCTTTTTTTTTTTCCAATTATTTTGTACTAATTTTGTTATTCTTTTATCGTTTTTTTTAATTGTGTTGTTATTATTTGTTTTGAAACATTTTAAGGGTTCGGGATTCCTATTAGTATCTTAGGAAGGTGTAAGGCACCCAAAACACTCCGTTAACTTACGGTTTTCCAATGATTAACTATTTGATTATTTTTATTTTTAACCTTTGCAAATTTATTTGAACTTTTAACATGACTTACTTAGCCAAACTTAGCCAATTTTGAAATATTTATTGTTTATCTTGCCAGTTTACGAAACCATTTCAAAGTTAAGCTTACTCAAGGTTTTATCTAAACAAGCCTTACGAATTTGAAACAATTTATCATTTAGAAGTTAGTTTTAAAAATTTTTAGGTTTGATAAAATAACAGGCGTTCGATGAGGGTTTGGAGTTTCCTAATCCTAAGCTTACGGCATTGAAAACAAAATAAGATAAGCAAATAGTAAAGAGAAGGATAGAGAGAGAGAGATTTGGGTCCAACTCCGGGTTCTGTTATCCTTGACCGAATGTTGGACCCACCTGCCTTTGGGTTTTTTAGCCCATTTCTGTACCTGTCCTAATCTAAA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 44
GEN 3040
CATACAAACATAATATAGAAAACAAAAAAACGATATTTTTGTGAAGTTAACTTTTTAATAAATGATAAATATAATAAATTTCAAAAATCAAATATATTTGGACGACTACTTAAATGTTTCAGTTTATTTTTTGAGCCAAATTTTTATCAGTGTGAATTGAATTGATTCAAAATTTGAAAAAGAAAAATATTTTCCACCTCAATTTTTCTATATATTTTGTGTAAAAATGATTTTTTTTTCCCGAAGTTCTTAAAGCAAACTTGACAATATCACAAAATATTTAAATTTCATTACTTCAAAATGATCTAAATATTCCTGCTGAATATGATGTTAGTTTATAATGAAATTAGACGAGAAATTCCTGAATATGACTGCCCACGTGAATTTTAGTTTTTCTTTGACAAAGAAAAACATGGCAACTACTTATTAATTTTTTTAAATTGATCAAATATTTATTTTCAAAAATAATTAATTATTAAGAGTATAATTAAAAAATTATTATAATTTATACATTAATTTTACAAAATAACAAATATTACGAACTAACTATTTTATAATAAAAATGACATATAAAAAGATACGGAATAAAAATAATCACATATATCAATAATATTACATCGGAATAATGATGAGGAAAATTTTCCACGAGTCACTATTCCACGTCCGCATTTAATTCTTCATGAATATTTTTTGGTCTTTTTGGGAACCTTCTCCTAACTTTACTCGTATCGAATTTAACTAAATCTAAATTCTTATTAAAAAATTCTATATTAAAAATATAATATTTGTGATTTAAAATTGTGAACATCTAATTTTTTATCAAAATATAAAGAATCAAACACCAATTTTTTTAAAAAAAGGTTATATTTAAAATAAAATAAAATAATAATAATAAATTGTAAATTAATAAAACTAATAAAATCAATTATAGTTTATTAATAAAAAAAGAATTGATAAATAGAAGTTGGGAATTTAAGTTATTTGTTAATTATCTAGTTTAAAAATAATTTAAGTTATTTGTTCACCTTATCCTAATTATTCCCAACTTTCTAACTTATTTTACTAAAAAGTTTCATTCCATTAATTTTGATTTTTTTTTCTTCTGACGTTCATTTGACTTTTTTTTTAATTTTTTTTTTAAATTTAATTTTCTATTATTATTATTTATTTATTTTATATCCTTATCTCAACCCCAATTCCCTCAACAATATTTCAAACTAAATCCTAATCCAACTCTATTACTATCCATTTAATTAGCATCAAACTCTCTCAGTTGAGAAGAAAACAGAGAATACAGACAAAGAAGAGACACAGACACACAGATACAGAGAGAAATATAGGGGAAAAAAACGAGTTATTTCTTTTGAGAATTAGGCTAGAGTCAGAAATTTGGAAATTTGATCGTGATTCCGAATTTGTGGATACAGCTCGCTTTTTGTGGTATCAATTTTCGCAGCGAGCTAACTATAAATTCTCGCGTGATTTAAATAATACTACTTCTATGAAAAATTGATTTTAATAGCAATCACATGTATTATATTATTGTTACGTTATTACTATTATTATGTTTTTTTTAGATAGATTTTGCAATTTATGTTGCTTCATGATATTGAGTTGTTGAATTTAAATAGATTAATATATATTTTCGTCTATTTTCACTTTAGAAGATTTTTGTTGAATTAATTGTATACTTGTTAGATTAGGATATAAAAATAATAACTAATAAATAATTTTTTTTCAAAAAGAAAAACTAATCTAAGTAGAATTGTAATTAAAAGGTAAATAAATTTCATTTAGAGATTAATATTATAAACTAATAATAATAATAAAAATAATAATAATAATAATAACAGAAGTAAAAATTGAGAAATGATATAAAGAGAAAAGAGATTTTAAAAAGAAAAAGAAAAAAAAAGGGGCATGAAAATATAATAATAAAAAAAACATAACAAAATTTTTTAACAAAAACAAAAAAAAAAGAACGTAAATTAAAAAAAAATACAAAAACGTAAAAAATAAAAAGGGAGAAGTAAGAAATAAAAAATAGAAATTTAAAATTAATAAAATAAAACGTTAAAAAAAAACAAATAATAACAACACAATTAAAAAAAACGATAAAAGAATAACAAAATTAGTACAAAATAATTGGAAAAAAAAAAGAAAAGAAGAAGACAAAACTATAAAATAATAATATACTAAAAATAATGATAAAATAAAATAATAAAAAAAAGAAGAAGAGAGAAACGTTCTTTCAGAGAAAAAAAAAACAAACAAATATATAAAATTTAAAATAAACAAGAAACATGAAAAAAAAAAGATTTTTCAACGTCTCGATCAAGGCCAGACTTGGACCATCAATGCACTGAGGGGCACTAAGATGGCACGTATATGGGGTCGTACTAAATGCAACTTTGACGGTGCTGGTAGAGGTTCTTGTGAGACTGGTGATTGTGGTGGAGTCTTGCAGTGTACCGGGTGGGGTAAACCGCCAAACACCTTGGCCGAATACGCCTTGAACCAGTTTAACAACCTAGATTTCTGGGACATTTCTTTAGTCGATGGATTTAATCTGCAAATTAGAAATTCGGGTAAGGAATTCTGTTGACCGAAGGGAAGGTATTAGGCACCCCTCGAATCCCGTGGTTCTAGCACGGTCGCTTTATTAACTTTAATATGACCTGACTTAATTTTGGATATTATATTTTTTAAACTAATTATAAAATTGTTATTTATCCTCAAATTTATTTTAAACTTATTTCAAACTGGCTTATTTATTTTAAAGGGTGTTTATTTTTATTTTTATTTTTGTGTAATTTTAAAATTGAACATCCGTATATATTTTTTTTGCTTATATTTGTATGTTTTTAATTTTTAATGTTATAATAGGTAGAATATATATAGATATCTAATTTCTAAGAATTAGAGTTTGTGTAAAAAGGACATATTTAGAGTTCAAGTTAATTTAGGATTACATATTTTAGATTTTATCGAGACTCAATACAGATACCAGATATTAATTTCTACTGCAGGGTCTATTTGGGAGTGGCGCTTCTAACATGATTTTTTTCATATCCAAAGCTCGAATTCGAGATTTCTGATTAAGGTAAAGCATTCTCACTAGTGCACCACAACTCATGTTGGTCATATAAAATTTTTTAATTTTTGTGATAATATTATTTTCTTCTAAAAAGTCTCACTCAATTAAGAATATTCTTAACACTATATTTAGCTTCTTCTATTTTTCAATTATATATTAATATTTATTTGCACATATTAAATTCATAATTTAAATTGCAATTGACCGCATGCAAATCAAAGTCTTCGTCAAATACGACGAGTCGTACACATGCCTTAAAATTTGAGGGGCTTATTTTTTTAGCAATAATATACTTATAAGTAATTTCAAAAGTAAGTATCATCTATTTCAATTTTTAGTTTAACAGTATAAGATTTAAATGATTTTTTTATTTAATTTCAATTATCATATCAAGTCAAAAAATAAGATAAATAAATGGAAAATTGAAAGTACTATTTATTGAATAAAAATAAACTATTTCATGTGAAAGAAAAAAAATAACTTTCCATCACTTTTTAAGAAATATATTAGTAGCAACTTTGTATTCTAAAAAAAAATATAGAAGAATGGACTTTCTTTAAATTTTAAATAGTACTTATTTGAATTATAAAATAATCTCAATTAATGTTCTATTTTTTTTCCAAGAGATATATTATGGTTTAAAAATTAATCCATGATGTGGAAAAATGATCAGGAAATTTTCCCACAAACCACTATATAACTTCTTCTTTTCTGTACTTTTGAATTCGATTAAATTAAAAATTTTGTTAAAAAATTCAAATTGAAGATATAACTTTCAACTTTAATGAAAATTTTAGATCAACATTTAAATTTGAGATTACTGGAAAAAAAATTGGCATGTATTACTTATGTAATAATAGTTATTAACTATTTGTTTTAGAATTATCAAAATT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 45
GEN 3040
CCAGTATAATTAGGTGATTCTTTTTTGGGACATTGTGGGGTTTCAACTTTCAATTTACAAGACTAGCAAGACTTGCTGTTGATAGGTTAGAAAAGTCTTTGTAAGAAATGAATTGTATTATTTTTCAAAAAAATTATAATATAAAAGAAGATATAAATACATGTGTAATGATATATTATCTAATTAAAATAATAAGTATTTTAAAGTTAAAGTATATTACTAAATATAAAAAAGATATTTATCATTTATTCAAAACTGATTAAAGAAAATCACATAAATTAAAATGAAAGAAATACAGCCTTATTTTCTCAAATTAGTGGAAATAGTTAAAATTTGAATTTGAAATAATGACATTGCAAACCCGTATTGTATATATAATATATCAAAAAGCTATATACCTATAGGGAAATATAATTAATATATAAATTTTTCTCGTCAAGTAAAAAAGTGTTAATACATATCTTAATATATAGATATTCATTCAAATTCAAATGTCTTAATTTTAAGGAAAGTATTAGAAACAATTAAATGCTTACAACTAAACGAAACAAAAAATTCAATCTGTCGGTGGCATTTACTGACACTAAAAGGGAATAATTAGAAATGTTCATTTTTTAAAAAATAAAATAAAAATAGAAATCTCCTATAACCAAAAACGACATTTTCTATTAATTTACCCTTTATATATTTTTTATCACTTTTAAAACACAATCCTAATTTTTAGGGAAAAAATAAATTAATTCTTCATATCTTGATTTTTAAATTGACTAGGATTTTAAAATGAACTTTGTCTTTAATAATGTTGATAATTAATATGAGACAAAGTCGGAGAATACTTAATGGCTTTAGTCATGTTGCTCAGAAAGAAATGTTTAATTAGTTATATACTACGGAAAAGGGTTAAAATATTCTTAAACTATCAGAAATAGATTTACCCTTAAATTATATTTTGATTTAAAACTATTTTTCTATCAAATTATTGGGTCAAAAATACCCTTCTTATTTTTTTTACCCTTTTCCGTATATATTTTTAAAAAAGACATTTTGATGAAAATTTTTAGAAATTGGAGCAAAAAGCATTAGATGATCATAATAATACATATTGCAAATATTTGACTGCAAAGCTAAGTCTTTGTCAATAATAAATATGAAGCTATTGCAATTTGTGGTCCTCATGCATTGGAAATAGTATGCATAGCCTTTTAATTAAGATGTACATTGAGTTACATAGCTGGAAAGTCGTCGTTTATTAAGAGTGTTTTATTTATTAATGTGAATTTTTTAAATACAAATTTATATTTAATCGAGATTTAATATGAATAATTAAACACCAAAATTTAAATTTAAGAAGGTACAATAATTTACCCCTCCTTTTGTTTTTAACTAGCTAGATCGAATTGTATATATAGGTAATCATAATATTCAAATTGTGCAATATTTGACTGTGTATAGCTAAGTGGTCAATTTATAGTTGGTCGTCATCACTAGAACTTTATAGCCTTTTAACTCTTCTTTGAAAAATTAAATTGATTAGAATACTTTAAAGAAATTAATTTAATTTCATGTGAAAAAGTAATTTTAAGCCACTTAATTAATTTCATGTACTTAATAATGGGAACGAGGAATATAAAATTATATTGGGTGAAGCTTCCACAAAGAGAATCCTTAATATTATAATATTATATAATACTTTTTGTTCTGAATTAAATCTCGATGATTTTTAAAATAAAATCTAGCTCGCTTAATTAAGAGTGTTCATAATGTTAATCGTAACTTCTCTTACTTTTCAATTATATGGCAAATGTGAAGTTTTGCACAACTAGAAAGCAATCTCCTTCTCGAATTAAGTTTCATGTATGCATGACTTACCACTTTATTCCTCGAATAATTTTACATAGCTCATCACTAAAATCGTCGATTCATGTGTTGAAATGGATATTAATTATCTGTCACGTATAGTTTTCAAATATTTATGATCATCGAAGTCCATATATAATGCAAGCTCCTATTATTATTAATTTTTGTCTAGCTTTTCATAGAGGGTGAAAGACGGATCAGACTCAATACCATGCATCTTCATACTCATCTCGTCAAAAGCTATTAACTTAAACCTTGAGGGAAACTTAAAAGTGACCAAATCATCATAAAGATATTCTTTATTTTCCTTTTATTCATCACAAACTAAGGAACTACATAGTACTTCCATATTATCACTATATATATGATGAATAAAAACATCATATTTTTCCTTGACTTAGTAATTAATCCTCCTAGCAAGGAGAATAACATAGCCTTATTCAAATTATTAATACAAAAGGATATATAGATAGGCAGATAGATATTTAAGGACAGAATACAACCCTATAATTAGTACCAGCAGGGCAAGTGAAAGTACTAGTTTGATCATCCTTAGGGTAACTATATGCATCAGGACACCTCTCCTTAAAAAATCTCGAAAATTTAGTCGGACCACAGTTACCAGAGTTACAACAATATTGATCAGTCTTAAAAACAGTACAAGGGTTGTTACATCCTCCGGGAGCTTTCAATTCATTAGGACATTGTTGATTAATTTCCGCTTTACACGTTATGCCACGGGTGCACCCATTGGAAGTTGGACTAAATTCCATAGGAACATTAAATCCATCGACAAGAGAAATATCGAAAAAATCTTTATTTGCAAATTGGTTTAGGGCATATTCAGCTAATGTATTTGGTGGTACACCAAAAGATTTACATACAAGAAGACCATTACAATCTCCAGTTTGGCATTTTCCCTTACCGGATGCATCGAAATTGCATCCGGTACGGCCCCAAACTCGAGCTTGTTTAGTCCCCGGAGGGGCGTTTATCGTCCACGTTTGGCCACGATCGAGGCGTTTGCCGCCTCCGGCTGGGACTCCGGCAGCCCAAACGGTGTAGGGACAATTGTTACGAACTAAAATTGTGGCTGCATTGGCATAGGTGAAAAAAAGGGAAAAGAGAATAAAAATTGAAGTTTTCATATTTTTATTTTTTGTGTGACTACTAATAAATTGGGATGAGTGATTTTTTATGAGGGAGTAATAATAATTAGAAGGGTATTTATAGGGTAAAAATGGGGCACCAATATAGTTGGTAAGTAACTAATTTGTTTGAAATTTTAGTTTGTGATAATGTAATGTAATGTTTTGAGTAGACTAGTACATTATTCTATCGAGGGTGCAAGGTGTGGCTACTAGAAGTTGTAGAAAATGTAGCTAGGGTCTATTATTCAAGCTTGGATTTTATTTTTTACTTTTGAATAATATGTATATATATATATATTTATTTTTTTTACGTATCAGAACGAAGTTCGATTAAATTTAGATACGCGCTTTAAAGTTGTATATTGAGTATAAAACGTTTCCTAACAAAAATAACTATGTACTTAAGAGACTCGAACTCGAAACATCTGATTAAGAATTAATGAAATAGAATACTTATCACTTCACGGCCAAAAGCGAAAAAGAAAAATCAAAGTCAAGAACAATGAACTTAGAATTTGGATGGTACATAAATTCAAACTTTTTTAGATAATCATGCAAGTTTAAGGACCTCTATTTAGCAACAATCGAAAGGCTATGCACACATGAACATTATATTGCTTTGTGATTTACATAAGTGACTTTTTTCAGTATCCACATTTGATATTCATATTGAATTTTAACACTCTATAAATAATAAAAATCGTGATTGAATTCGATCGAAATCTTAAATTAAAAATTTGAATCAAAATTACTGAATTCTTATTCGTCAATATTTAATGATCTGTATTAGTGCCCCTCGAATCAATAAAGTTTAAAAGATGGTGACTTGTTAAGTGTACAAAAAAATCAAAAGAAAAATAGAGACTTGAAAGTAAGAAATATCAAAGTTTACTAAATATGAAAGAAAATTGTTAACTACCTTGTACAAAATTAGGTGGTGGAAAATTCGTCTTCAATCATATAAAGGCATTTTTATAAAAGT
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 46
GEN 3040
TATGTCATAGACAAATGACTGGCCAAAAAGTTATATATTTGCTAAAATAAAAGGTTTAAAATTAAATAATTTTTAAATATAAAATATATTAATCTTTATGAAGTAGATTAAAAAGAGATGTATTTATATTAAAAAGAATAAAGACTTTTAAAAAAATAATTATAAATAAGAACAAAACTCGAATAATATAGGAAATTTGTAGAAAGGATAATGTCTCCAAATAAAATATGGGACGACTCAAGCAAACAGTGCTACAATTCAAACGCAATTAACAAGTGAAATAAAAAATTTGTCAGAAATTTCAAAATCAACAAACGATTTATGAGGTTCAAAAATTTATAAGAGTTTCATCTTTCTTCTGCATTCTAATATCTCATTTCCAATAATACGTTTTAATACACTTTGTCTCAAATACAAATAGTAAATGAGTCAATATATAACGTAAAATAACGGCGATATACGATAAATATGGGAAATCTAAGCATGTGTGAACATAACACATAATAAGTCATCCATTATTTACTGATTTATGGTAAATTAAGTAAGGAAGAACGTGACATTTTATTATTATTTAATGAAAGAAAATACTTTGAGAATATATTAAACATGACTAAGAGAATTCAATATTTTATACATTTTAATTTATGGCACATTAATATAGTTTTTCCACTTCTGTAAAAAATATTTTAACTTAAATTACACTTTATCTAGTTTCTAAAGACGAAAATATTAATTTTCAAACAATAATTAGTCTAAACTGATTAATTGAGCTAAAAAGGTAAAAATTGATGAGATTGTATACCAAGAAGATTATTATAAAGTAGTTTACTATGAGAACATATTATTGCAAATGAGTGTATTGATACTTTAACCTAAATTAATGTGCCAAGCTAATGTATTTTTTATCAATAGTTTACTGGACTGATATAAAAAATAAAGGGATTTTTCCCCTCGAAATTTTGATCAAAATTTATTATAATCGTATTTTATTTATGTTATTTTATGTTTTAGTTTTTTCTATTCTTTTAATTTTAATAGCTTTTTATTTTAAAAAATTAATTTATTTTAAAATAAAAAATATATTTAATATTTAAAAAAAATTAATAAAATTTTCCGACCATAAATAAAATATCATATGCAACTTATAAATTAAATGAAAAAAAAAACTTATTTAAATGTAAGGAATATTTTTAAAATTTAGCTAAAAAGTTTCCCAAGTTACGAGGGAGGGAATATGGATGTTCATATTTTGGATAAAATTGATTCGGAAAGCTCAATCAAATTGATTAAACAAAATAATTTGTTTTTTATTTAATTTTACATTTTTTAAATTAGATAATAGTTGGCTTTCCTTAAAAAGCTCAAAAAAAAATTAATCAAATATATATTATGAACAATTTTAAATATTGTATATATGCTTTCGTCAAGATTTATATATATAAAAATTGTTCAACACAAGAACATCTATTTTATGTATCAATTTTATTTATGTGAGTTTTTTACGAGATTAGGAATGTCATTGCCACTTCCTAGTCAGGGACAAAGTGGTGAATTTCAACGCTTTGTTCACATTAAATTTAGTCTTTGAAAGTTTTGTAACGTCATCCGATCTAATTATTTTTTTGTTTGAATAACTTGTTAATTTTTTCTTCCCTTTTATATGAATCAGTTCTTTTATGTTTTATATATGATTAATAACTGAATAATCGAATAAAATCACATTGAAATCGATAACAATCAAGCTGATAAATGTATATTATATTAGGTTTGATTTGATTTTGATTTTGATCAATAACTAACCCTAATCGACTTGTGAACACTCATAGTTGATGATAGTAATGGTTGTAGTTGAGTTGAGGACGGTAGTTATGGACGTTAGATGATGGTGATAATTATGACTGAAAATAGATGAAACAGTGATGATTTGTTATCTCTATAAAATTCTTAAATAATAAACATAAGACTTCCATATAAATTTCAATCATTCCGACTCATTTAGACCCATTAAGTACGGTAAAGATCAACACGTGAGTAATTAAAAACTCATTCACAGTATTTCAAACTTGCATTTGTTAACATTAAACATTTTACAAAACACGTGAATTATTAACCATGCAAAAGAATTCCAAATTTGCAGAATTCGACCATCCTTCTGTGACTGAATTCAATAAAGTCATATCGATAACTTTTAAATCCGTTACTAACAAAAACTATTTGTATGCATATATACAAAAAAAAATTGGCTTATTTTTTTAGTCTAGTTGAAAAATATATTATCTCGAGATAAATTATTACCCTAAAGAGAGACGGAGCTAGAAATCGAGTAATATAAAAAAGAGGTAAATGAAACGGCGTTAAAAAAAAAAAGTCCTTCCTCAACCCCACAGCTTGAGTGTGTTTCTTCTATAACACACTACTTTTGTATATATATTTTCAAATCCAACAAATCCGTCAGTTACTCTTCAGAATCTTCTTCAGTTTCCCGCTACTCTCTCTCTCTACAAATTAATATCTTTAAACCCTAACTTCACTCAAAAACGGTCAGTTACATTCAATTATACTCTCTTTCTCTCTCTACAATTTAGGTACATTTTTTTTCTGATTTTTATTTAGATTTTACGAATTCGAAGCAATTTTAGCTGTTTTGTTCATCATTGTATGTTTTCAAGCTGTTTTATTTTGGATTTTGCGGTTTTTTGTTCGTTCGTTAGGTGTGAATCTGGTTGTTGTGAATTTTTGATCCTGTTCGATTGATTGATGGAATGTTAGTGTAAAGTTTTGATTTTTTTTAGTTAAGAGATGTAAAAAAGGGGATTTTTTTGTGTGTTTTATGCACCTGGGTGCATCTATGGAAAGGAATAGATATATGAAAATTGTATTGGAATGAGTGAGTTTGAGTGGTTAGGAATGATTTATATAGCGGATCTGTACTGGTTTGGGGTTCAGGTGTACTTCACTGAAACAGTTCATTTAATTTGACTACTCTGATTAGCTATATCTGTATCTAGGTTTTAGGTGGATTTTTATGTGTTTTATAAATTTGTTTTTGTATCCATTCCCTGTTTATTTCGAAGCTTTTCAAGTGTTTATCTGCATTTCGTGGCATCTTGTTCATTATTGTATGTTTTTAAGCTTTTTTGGGGGGAATATATTTTTTTTTGCTTATTTGTGTTTTTTATTAAGGGGTTAATTCTCTTCGTTTGGAAAATCTCGTGATGTTTGATTGCTGAAAAAAATGCTAGTTTAAAGTTTTGATCTTTGTGTTGAATTTTGAGTTTTTTTTGCTTAAAATTTGGACTTTTGTGTGTTCTAAGGTTCTTGAGGAGGTACTTTAGGGATATTCATGAAATTGAAATTATGAAGTTGATTTGTTTGGCTACTATGCAAAAATGATGTTCGTATTGCATAAATTGATTTATATTGGGATTGAGCTTGAGTTGATACAAATGATTTTGATAGAAGATCTTTACTAGTTTGGGGTCAAGGTGTGGTAATTGATTGATTAGTTCCTGAGGAGCACACACATCTTTATTGTCTTTCTCTTGCAGAGCTGTGAGATATTTGATCGTATTACTGGATTGTTGGGAATTCTTTCCATACTTTTGTTTGGGTGCCTTGAACATTTAGGATTTATGACATATGTTTGTTTTGTTGAAAAAGATTCTATTTTCTTACTTGGTGTAACTAATAGCTCTCGCGACTTCCATGTCTAGATAAAAGTAGAGGGTTATAAGTTGACAGCTAGTATAAACGTAGTCTAGCATTATACTGATTGTCGGGCACAACATGTTTACTGATGCGAATAGAGCAAAATGTGGTTTTAATTTCTATAACTGTTCTTGTTTTTCTTCATTAGCTTGTGCATTTGAGCATTTGTTCTTACTTCTTCTTTGTCGTAATTGTTTTGCAGTTTAAGGATATTCTTTGGCTGCAAGACATGGTACAAGTTAAAGAGGAAGCTCCAACTCTGGACATTGGTGGATTCATTTC
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 47
GEN 3040
TTGTTCATCTCTCTCAGATAGCAGTTATGAGGCTAGCACTCCGAGATGCTCCTCCGAGCCTGGGTATGCTGAAACCTTTGATTTTTTTCTGTCTTTGAACAGTTGGGCCTCAAATAAGGTCTTTCAAACAGATACGCTGATTGATGCAATTTTAGTTTTCACTTCAATTGTTTAATCCTATTTCAGTTCCAGTTGTCGAAGGAGCTCAGGTCCAACTAAGCGTTCTTCTCAGGCAGGCTGGACAGAAGAAGAGGTAAAAGGCTAGTCGCTAGAGCTTTTGTTGTACCTATATGTTGTTTGATTTCTAGTGCATTAATTCTCCATTGAAGTATCCTGCATACATGTATGATGGTTTTTATAACCTTGTGATGAGAGTGTGTCTCCGTGATAGTTAGGTACTTATAGAAGTATCTGTATGAGTTGTCATCTCCTTTTTCCTTTTGGGTTCTGATGCACGTGGATGCTTAGGATGTATAATTGAGCTAAGGAAAGGATAAAGATAGATTTTGTTACTCATAATATCAGTTTAATTTACTCATTTTTACTATTTCCATAGGCTCATTTTCTTTGATCTAGAAATTTCTGACATCTTTATTTCTTTCACAGGACAATCTGTTGACTGAAGTGGTGGAACGGTTCAAAGGAAGAAACTGGAAAAGAATTGGTAAAACTATTTACTCTGTCATGCTCTATACTTTTCAGCTAGGTTAACCACAAAGACCTTCACTTTTTGCTCCAAGTCACTTTATGACTTGGTTTCCATATTTTGAATACCTGACTATTTCATGGAGTAACTTATACTGTTGTATCTATGAGCAAAATTTGTCTGCTGAACATTTTGTGTGGTTTTATTGTGTTCTCTTTTGTTCTATAGCTGAGTGCTTGAATGGAAGGACTGATGTGCAGTGCTTGCATCGCTGGCAGAAGGTTCTGAATCCTGAGCTTGTAAAGGGTCCTTGGACAAAGGAGGTATTAAACAGTTCTACATCCATTGGTTTTCTACGAGTTGTATTCCAATTATCAATACTTTGATTATTAAAGACAACTGTTTTGTTCTATTTGGAATTTCAGGAGGATGACCTGATTATTGAGTTAGTTGAGAAACATGGCTGTAAGAAGTGGTCCTCTATTGCAAATTCTTTGCCTGGTCGCATTGGCAAGCAGTGTCGGGAAAGGTCAGTTTCTAACTCCGCCTAAATCTGTTGCTAGGCCACAGCTTCTATTTATACCTCCGACTAATTTACATGATCATAAACCCAGAATTTTTCAAAAGGCTTCCTGGAACATCTGTAGTTATGTTGCTTACAGAGTTATTCCTTGCCATCAGAACTCATTTTGTCTCACTTTTCTCTCGTGTTTTGCAGGAATTGTATCTTTTTGTCTTATTTTCACTTAGTCCTACTGGATTTTTTGTCCTGTTGAGGCCCTGCTCGTTTGTAGGAGCATTGTATTTCTTGTTATCTATTGCTTGATATATTGTGTGCGTCTTTTCAAAGCAATTAATCAATCAACTACTGCCCCAATTCGTAGTAGGGGTTGGCTATATGAAAAATCCATAATTTGGGGCACAACTCTTCTTTATAAGGATTCAAACTGGCTATGTTTTGCACATAGGCAGATTTCTCTTTTGTAAGTGAATAAGAAAAAGCATGAAAACTGGGAGCCTGCTAAATATTTGGTTACTGCAATTTAATGGTATATTATTCGTTGGATTTTGCAGTTAAAGCTTAAAAGGAGATCCACATTTCTATGTTCTGTTCCTTTTGAATAAGAAAATGACATTGGTTGATTAATTGCAAGTTATCATAGTTGTTTCAGTCTGCTATGTTCACTAACGTTGTGAGATCGTCCATGAATGTCACAGGTGGCATAATCATCTTGACCCAACAATAACAAAAGATGCCTGGACTGAACAGGAAGAAATGGTCCTATGCCACTATCACCAATTATACGGAAATAAGTGGGCTAAAATTGCGAAGTTTCTTCCTGGAAGGTATTCTTATAAAACTATTCTTCTATTATGTATATCAGTGTCTGATTTTGAGATTCCTTGGGCATTCGTAAAATGTGGATCAAGTAATTTGTTATATCTATCACCCTCTCATACCAATGGCAGATCGTTTGGAGTGCATGATTCTGAAAAGTCAGTATTAGTGTGGTATAACTTGTATGCCAAATCAAACACAACTTTTTGATTTTTAGGTTTCCTTGGTACATTGCTTACAGAGTGTATCAAAATCTTGGATACTACTCGAGTTTGTTCTAGGGAGATAAGTAGATAATTTTTATAATGAAGGCAGTGAAGGCATATGCAGCTTTCTCTTTACTTTTACTTCTTTTTATCTTATTCTCCAGTTTCACTTCCATCCAGACTTCTAAATCATTTTCTCCCAATATGGTTTCTTCTTTTACTTCTTGAGGTTCAAAACTATTCATGTCTGGAACTCTCTTAATATTTTATTTTGTCAATTTGACATGGAAAAAAAGTTGAAAGAATCATTTTGGGCCTAACTCCACCCCAATATCTAGTTCAAGAGGGAGGAATGCCCAAGACTATGTATGTAGATCAGCCATCTCTTTTTCATTCTATAGAGGGCTCTTAAACACTCTCGTCATGCCCAGGACTGAACATCAGGAGTATGGGCAATTCAAATAGGGGCCCAACATCGGGAAACAAGAATTGGGATGAGTTCTGGTATCACCAGGTAAAGAAATGGATCTTGGGACTAACTCAACCCCAAAAGCTAACTCATGAGTGGAGGATTTCCAAGGCCATATACGGAGACCACCTATCCCTCTTTCACCCTATGTGGAACTCTTCAACAATTTTAAACTTGTAGTAATAAGCTAAGTTTTTTTTTTTTGGTCAGATTGACCCTCGGCAAGCAAGTTAGGAGAACCAAATGGGGACAAAGTTAGTAATAGTATTATAACTTCTTTCTGCAGAAGCCTGGATATTCTAGGTATCGTAAGTGTACTTTTAAGGACTCAATCGTGCACTCTGTTTAATTCAGAGGATTGTCGTTCTTTTGTGCTAATAGCTGCAGGTACATGTGTCAATTTGTATCTATTAAGTCCAGTTTCTAATTTATCATGATTACTCTAAAATGATTTTTTTGCCTACGCTTGATGCGTTCTGCTTGAATGATTTTTGCTCAAGTATCATATAGGTACATATCTTTGATTAGTTCTCCTTTCTGATCTATTTTTCCTTTTGGCACTCTCTCAGGACTGATAATGCAATTAAAAATCATTGGAATTCCACTCTGAAGAAAAGATTGAATTTGAATTTACTAAGTAGGTTAGTGCCGGATATCAAAAGTGAGGGATCTCCGGACTTCTCAACTCACAAGAAAAATATGGAAATCAAGAAGCATCCAACACAGGCTCATAATGCAGAAACAGTCTTTTTGAGCGAGCAAACAGGAGTAGGTAATGCTGCTGATGCTTGCCCAACTGATTTGAGGATTGGATCTGCTAATTCTCCTCAAAATGGTTTGCATAAGGGTACTTCTTCATTGGGAACCTGTAAATCATCAGAACAGAAGGAGAGGGATCTGATAAAGCAATTTGGTGGAATACAATTTGTCAAGGCAGAAATTCTTCCAATAGGTGAGACAGATAAACAATGCCAATCCACTTTAAGTCTCACTAAAATATCATATCCTCTGTTGGACTCTTCTTTAGATGCTCGTTTGGATCCATCACACAACACCGGATGGAGTAGTTCTCAAGTTGAGGCTGTTCGTCCTACTTCTTTTGGGAGTATGTATGAATCTCCCAAAAGGTCTAGGTACGATGCTGCCAATGATCCTGACCATGATTTCTTGAGTTTGTCATTGGCTGCCTTCACCGAGGTTTCCTACGGTAACAAGAAAAACAGAACATATGATACACAATATTCTTTGGCTCTCAATCAGCCTGGCCGCTTGTGTTATGAACCACCAAAAGTAAAAGACCTGATGATTTCTTCAATGGATGAAAAACTTA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 48
GEN 3040
GTAAAGACAACTTTATCGGGCAACATCATGGCCATCCACTTTGCTCTACACCTCCTAGTCTTAAGTTAACAGTCTCTGCTAATGGTAGCAGTCCAGAATCTGTGTTAAGGAACTCCGCAATGAGCTACACAAAAACTCCTTCAATCATAAGGAAGAAGAATTC
Figure 10. The cladogram tree of the genetic relationship of the cloned sequence and five
others using ClustalW
Figure 11. The phylogram tree of the genetic relationship of the cloned sequence and five
others using ClustalW
Q3. Have both the cDNA-derived sequence and the genomic sequence of this gene been
determined?
Yes. Both the cDNA-derived sequence as well as the genomic sequence of the Nicotiana
tabacum osmotin gene were determined. The entry number of the genomic sequence in NCBI os
X95308 and the cDNA is M29279.
Q4. Are there likely to be any post-translation modifications of this gene product? What are
they/what is their purpose?
There are post-translation modifications of gene product and the they are the protein motifs at
specific sequence found in the amino acid. For this sequence, the sequence motifs were
determined by using Patmatmotif program
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 49
GEN 3040
(http://inn-temp.weizmann.ac.il/emboss/output/220946/outfile). The protein motifs found were
EGF_1, THIOLASE_3 and 2FE2S_FER_1.
######################################### Program: patmatmotifs# Rundate: Thu 19 Aug 2010 20:00:04# Commandline: patmatmotifs# -auto# -sequence /var/www/html/emboss/output/220946/.sequence# -nofull# -prune# -outfile outfile# -rformat2 dbmotif# Report_format: dbmotif# Report_file: outfile########################################
#=======================================## Sequence: from: 1 to: 1304# HitCount: 6## Full: No# Prune: Yes# Data_file: /usr/local/share/EMBOSS/data/PROSITE/prosite.lines##=======================================
Length = 12Start = position 1271 of sequenceEnd = position 1282 of sequence
Motif = EGF_1
GTCGACTCTAGAGGTCCCCGGT | | 1271 1282
Length = 14Start = position 1053 of sequenceEnd = position 1066 of sequence
Motif = THIOLASE_3
TTCTGAAGAACTACATGGGTCCGG | | 1053 1066
Length = 14Start = position 1016 of sequenceEnd = position 1029 of sequence
Motif = THIOLASE_3
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 50
GEN 3040
GGAGTAAATACAATATAAATTCTT | | 1016 1029
Length = 14Start = position 830 of sequenceEnd = position 843 of sequence
Motif = THIOLASE_3
CAGAGAAGAACAACACAGAAGATT | | 830 843
Length = 14Start = position 85 of sequenceEnd = position 98 of sequence
Motif = THIOLASE_3
AGGAAGAAAACAAAAGAAGATCTC | | 85 98
Length = 9Start = position 1244 of sequenceEnd = position 1252 of sequence
Motif = 2FE2S_FER_1
ACTACCAGAACATCAACTC | | 1244 1252
#---------------------------------------#---------------------------------------
Q5. How many amino acids are present in the mature protein?
By using the Expasy-Translate program, there were 246 amino acids were found in the sequence.
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 51
GEN 3040
Q6. What is the molecular weight and the isoelectric point of this molecule?
The molecular weight and the isoelectric point of the molecule were obtained from ExPASy
Proteomics Server by using Compute pI/Mw. The isoelectric point obtained is 5.09 and the
molecular weight is 109296.44.
Compute pI/Mw
Theoretical pI/Mw (average) for the user-entered sequence:
10 20 30 40 50 60 CGCGCGAGCG CCAACGGTGT ACGGGCAGTT GTTTCGGACC TCGATAGTTG CAGCATAAGT
70 80 90 100 110 120 ACAAGTCACA AAGGCAAGGA GGAAGAAAAC AAAAGAAGAT CTCAAGTATT CCATGTTTGT
130 140 150 160 170 180 GGACATTTTC TTTTTAACAA GTTGCGTTGT TGGATATAGT GACAAATTAT TTGGTGGTTT
190 200 210 220 230 240 ATATAGGAGT ATGGCGGCTT TTCGCACATA GACATTAATC ATATATATAT TTATTAACAT
250 260 270 280 290 300 TTGACTAGTA AATGGATAAT TCTCAAGGAT GCTTTAGTAA AGGTGGAGGC GGCTAAAAGT
310 320 330 340 350 360 ATAAGAGCCG CCTAATAAAC TAATAATTTT ATGAATATAG AAGTCAATTT ATTTGGTGAT
370 380 390 400 410 420 GATGCTGACC TATAAGCTCT GTAACGTGAC ACTTGTAACA ATATCTCTTT TAGTCAATTT
430 440 450 460 470 480 CTTGCTTTCT TTTATCTATA CCATTTTTTT AATTCACAAG AAATTGGCAG ATAGCCATCA
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 52
GEN 3040
490 500 510 520 530 540 TTTTGAAGCT ATTCAATATT AGTTGCTTTT TAACATGAAA ATAAACTTAA ACAAAAATAT
550 560 570 580 590 600 TTTTTCGCAA AAATAAGAAA AATATATAAA ATTTTTATTA GAGTTTAAAT ATTTATCAGT
610 620 630 640 650 660 TTGAACTTCA TATTTCATAC TCCCGAAAAT TTTTATGGAG TTTGCACAGA TCATAAATTT
670 680 690 700 710 720 TTCATGTAGT TTTAATACTA AATCTTGGAG TTTCACTTTT AATATTGATG TTATATTGTC
730 740 750 760 770 780 CCCTACTTAT TTTTTCTTCC TTCGTTTTCT TTTTGAGAAC TATTTCATCT ATTTATATAT
790 800 810 820 830 840 AGCAGCTCAT TAAGTAGATG GCGTTATTCT GGATGTGGAA TAAACAGAGA AGAACAACAC
850 860 870 880 890 900 AGAAGATTAA TGCCAATCGG GTAGACTTGA TCAACAATTT AACAAATAAG CTGATTGTAG
910 920 930 940 950 960 ATAACATAAA CTCATAAAGT TACGCCAATT CAATAGAGTT TGTTGAACAA TTGAACTAGC
970 980 990 1000 1010 1020 ATAAAATCAT GCCAACTGGA TAGAGTTTGT GAACACTTAA CAATATAGGT GGAGTAAATA
1030 1040 1050 1060 1070 1080 CAATATAAAT TCTTCCGATA GAAAAATTTC TGAAGAACTA CATGGGTCCG GAGATCATTT
1090 1100 1110 1120 1130 1140 TTTAAATATA TTATAAAATG GAGACAATAT TTAAAGACCA GTCCGTTATA GGCCGGCCAT
1150 1160 1170 1180 1190 1200 TAATTCTTGT CCATCAAATT TGCATCTGCT GTTGGTTTTC TGCTAGATCT ACTTAAAATT
1210 1220 1230 1240 1250 1260 TATAAAACAA TCAGTTTCGA AACAAAGTCT AGTGTAGTAC TACCAGAACA TCAACTCATA
1270 1280 1290 1300 TAAAAGTCGA CTCTAGAGGT CCCCGGTACC GAGCTCGAAT TCGA
Theoretical pI/Mw: 5.09 / 109296.44
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 53
GEN 3040
Q7. On an annotated printout of the promoter sequence highlight and list the features common to
the regulation of eukaryotic genes. In addition, using web-based promoter analyses too and your
knowledge of gene transcription/regulatory machinery and the references listed, locate and
record the position on a printout of the above file any sequence motifs in your promoter
sequence which may be important in regulating the expression of this gene?
A promoter is a part of the DNA that is essential in the transcription of a gene. Generally,
promoters are located on the same strand close to the gene they regulate. The RNA polymerase
must first attach to the DNA sequences close to the gene for transcription to occur. The
promoters contain specific DNA sequences and response elements which provide a binding site
for the RNA polymerase to attach as well as for the attachment of the transcription factor. The
location of the promoters is usually adjacent to the gene which is near to the transcriptional start
site. If the gene sequence encoded for the promoter goes wrong, the amino acid could not be
produced. As a result, the production of the protein will be affected and the wrong promoter
sequence will regulate the expression of the gene. The sequence motif or the DNA-binding
proteins in the promoter region regulates transcription, polymerases, histones as well as
nucleases. They are also capable of incorporate the domains that aid the binding process to the
nucleic acid such as the leucine zipper, zinc finger, and helix-turn-helix. The DNA-binding
proteins involve the chemical modifications such as methylation, acetylation and phophorylation
that could make the DNA more or less accessible to transcription factor. As a result, it affects the
rate of transcription process. The DNA-binding protein also regulates the expression of the
osmotin gene. The TATA box is essential for the initiation of transcription and bind to the
specific region.
The sequence of the TATA binding protein can be obtained by using fuzznuc (http://inn-
temp.weizmann.ac.il/emboss/output/470819/).
# Program: fuzznuc# Rundate: Thu 19 Aug 2010 20:29:38# Commandline: fuzznuc# -auto# -sequence /var/www/html/emboss/output/470819/.sequence# -pattern TATAAA# -outfile outfile# Report_format: seqtable# Report_file: outfile########################################
Plasmid isolation, automated DNA sequencing and bioinformatics analysis Page | 54
GEN 3040
#=======================================## Sequence: from: 1 to: 1304# HitCount: 5## Pattern_name Mismatch Pattern# pattern 0 TATAAA# # Complement: No##=======================================
Start End Strand Pattern_name Mismatch Sequence 565 570 + pattern . TATAAA 1024 1029 + pattern . TATAAA 1092 1097 + pattern . TATAAA 1201 1206 + pattern . TATAAA 1259 1264 + pattern . TATAAA
#---------------------------------------#---------------------------------------
#---------------------------------------# Total_sequences: 1# Total_length: 1304# Reported_sequences: 1# Reported_hitcount: 5#---------------------------------------
Q8. Why is EDTA included in the GTE buffer?
EDTA is responsible in the removal of the magnesium ions. The ions must be removed as it is
essential for the preservation of the cell envelope structure. EDTA in the GTE buffer works by
protecting the DNA from the enzymatic degradation in the chelation process of free divalent
cations and also capable in causing the bacterial to burst (Brown, 2006).
Q9. A manual sequencing autoradiogram of the Sanger dideoxy reactions often has a region
where there are terminated fragments in all four lanes with a fainter sequence continuing past
this point. Suggest a reason why this may occur. What might you expect to see in this region of
you sequenced this DNA fragment using dye labeled dideoxy nucleotide terminators?
There are terminated fragments in all four lanes with a fainter sequence continuing past this point
as it might be caused by falling of the template by the DNA polymerase. The phenomenon can
be explained by the secondary structure of DNA template, the hairpin loop that leads to the
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falling of DNA polymerase. As the replication happens, the sequencing fragments in the manual
sequencing which are visualized by radioactivity attached into the sequence fragments and
causes the premature fragments termination. As they have no labeled terminator, the premature
terminated fragments will not be able to be observed. The secondary structure will not always be
carried by the template molecules as DNA breathes in solution, the template molecules will be
able to overcome the hindrance and fragments in the regions by the aids of some DNA
polymerases. Thus, it will be terminated by the correct labeled nucleotide. The termination will
be appeared as a reduced peak in the automated sequencing file.
Q10. Perform a visual inspection of the following DNA sequence. Can you suggest an
origin/function for this sequence?
The presence of the CAAT box indicates that the short sequence is the promoter region with the
coding sequence. There are also a few sites for the restriction enzymes in the sequence.
Q11. You have a large linear fragment of dsDNA of unknown length. To determine the size, you
have digested the fragment with three different restriction enzymes that have six base pair
recognition sites and run the resulting fragments on the agarose gel. The three digests gave 5,6
and 7 fragments respectively. Having forgotten to run size markers you are forced to estimate the
length of the original fragment (assuming a strictly random sequence). What is your estimate of
the length in bp’s?
Assume that the four nucleotides occurred randomly, the possibility of the occurrence of the
recognition site for the restriction enzyme is 46=4096. Thus, for each 4096 bp, there will be a
restriction enzyme. The restriction enzyme digested 5,6 and 7 fragments with an average of 6.
Thus the sequence size will be 6 x 4096 = 24576bp.
Q12. The sequence is 5’CAATTTCACACAGGAAACAGCTACGACCATG 3’.
Q13. The experiment can performed following the procedures outlined follows: the gene of
interest is inserted into the plasmid after it is isolated. The gene of interest is ligated into the
plasmid as the vector for further step, the amplification. The plasmid is cut by using the same
restriction enzymes used to cut the gene of interest. The EcoRI and SalI restriction enzymes aids
the identification of the insert into the plasmid. The DNA segment will be ligated into the LacZ
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portion. The transformed plasmid will be grown on the X-gal plate. Colonies with white color
indicate that the β-galactosidase enzyme is not produced as the lacZ gene is disrupted. Contrast
to the normal/undisrupted lacZ gene, the production of β-galactosidase caused the cells to turn
into blue color colonies.
The white colonies will be isolated and extracted where the successful transformed
recombinant cloned will be cloned and digested by using the restriction enzymes such as EcoRI,
BglII and HindIII to produce different fragment sizes. The different fragment sizes can be
separated by using agarose gel electrophoresis that will be loaded with the marker gene. At the
same time, another set of digest is performed by using the original plasmid that is also digested
by similar restriction enzymes. The results of fragmentations will be compared to each other
(Glick and Pasternak, 2003).
As the vector orientation is identified, the DNA sequencing can be performed to ensure
that the size is 400bp. The fragmented plasmid is isolated and the agarose gel is purified. Next,
methods such as chain termination, chemical degradation or automated sequencing can be
performed to identify the sequences of the fragments. As only single stranded DNA is required,
chain termination method is preferred. BglII and HindIII restriction enzymes will cut the 400bp
fragments from 5’ to 3’ direction. The sequences obtained are shown as below:
5’ A↓GATCT……………..AAGCTT 3’
3’TCTAGA……………...TTCGA↓A 5’
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References
Brown TA., (2006). Gene cloning and DNA analysis: An Introduction 5 th Ed. Blackwell
Publishing, UK.
Glick BR., and Pasternak JJ., (2003). Molecular Biology: Principles and Aplications of
Recombinant DNA 3rd Ed. ASM Press, US.
Sanger F., (1981). Determination of nucleotides sequences in DNA. Science, New Series
214:1205-1210
NCBI (National Center for Biotechnology Information) (2010) BLAST.
URL: http://www.ncbi.nlm.nih.gov/blast/Blast.cgi
Date accessed: 15th August 2010
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