Tags: Imaging analysis, creative proteomics

Introduction for the Imaging Analysis

Summary: Imaging analysis is the extraction of meaningful information

from images. It mainly from digital images by means of digital image

processing techniques. In this article, we mainly introduce the service--

imaging analysis the Creative Proteomics offers. And if you are in need

of this service, you can contact us, and we will appreciate your coming.

Body：Imaging analysis of 2Dgels can offer all kinds of types of

information for the detection of novel missing, or modified proteins;

quantification of protein spots; determine of pI and Mr values of protein

spots enzyme digest and mass spectrometry detection.

For the complex 2Dpatterns’ evaluation and comparison, the gel images

have to change into digital data with a scanner or camera for further

analysis with computer software. Usually, a pixel format of 100mm is

applied for scanning, higher pixel resolution, which will not improve

imaging analysis and the image files, will become too big to be processed

in a right time and take up a lot of space on the hard disk.

In imaging analysis of 2D patterns, a background subtraction function can

be easily lead to errors. It has been found that the relative spot volume

quantification comes very close to the real situation and ensures a very

high reproducibility by defining the spot boundary at 75% of the peak

maximum and calculating the spot volume for only the values above the

boundary. The all is found after many years’ experience in the spot

volume quantification.

The scanner must be calibrated by using a gray step tablet. The measured

dimensionless intensity is converted into OD values or absorbance unit.

According to the data of imaging analysis, for accurate picking of spots,

the x/y position need to be absolutely correct in the mm tolerance range.

Usually the x/y with the help of a grid have to be calibrated for each

scanner. These calibration data have to be imported into the spot picker

computer for each scanner used.

For differential analysis it is important to be able to superimpose the spot

patterns. This can not be done directly, because there are local distortions,

which is caused by imperfection in gel matrix, variations in gel running

conditions, temperature effect, uneven focusing, and polymerization

issues. Formerly spot matching tried to find corresponding spots in pairs

of gels, but it is more efficient to search for pairs of features for automatic

matching. For these process, it uses spot clusters, spot shapes, sizes, and

position together. However, even this feature-based matching can

sometimes produce mismatches, so controlling this process by visual

inspection and performing corrections with setting landmarks are very

necessary.

About the author: Creative Proteomics is a supplier for the biological

fields, and it has a lot of services in its, it has many experts in this fields,

and they all are experienced in this field, and also give a lot of benefit for

our company. So you can find the service you need in our company, and

we also promise that we will provide you the highest-quality service for

you.

Links: http://www.creative-proteomics.com/Services/Imaging-

analysis.htm

http://www.creative-proteomics.com