introduction permeate recycling · introduction title text permeate recycling harvesting and...

Harvesting of a fragile alga Dunaliella salina by membrane filtration with permeate recovery for microalgae cultivation

Joana Montea, Marta Sáa, Celina Parreirab, Rui Motab, Joana Galanteb, Pedro Nascimentob, Filipe Semiãob, Cláudia F. Galinhaa, Vanessa J. Pereiraa, Diana Fonsecab, Luís Costab, Vítor Verdelhob, Fons Jacobsc, Carla Brazinhaa, João G. Crespoa

Introduction

TitleText

Permeate Recycling

Harvesting and Monitoring

ThisprojecthasbeenfundedbytheEuropeanCommissionunderthe7thFrameworkProgrammeforResearchandDevelopmentCollaboraAveproject–GAno613870

www.d-factoryalgae.eu

D-Factory Partners P Harvey, P Lucas, V Verdelho, L Costa, D Fonseca, D Rooke, C Bright, F Jacobs, P Goacher, J Crespo, C Brazinha, G Reinhardt, H Keller, L Martinelli, D Schroeder, N Igl, A Kokossis, M Psycha, A Ben-Amotz, K Persson, L Brive, I Mira, D. Pen ̃alosa, C Casanovas, D Arroyo

Contact Details Carla [email protected] , Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal

aiBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal bA4F – Algae for future, Campus do Lumiar, Estrada do Paço do Lumiar, Edif. E, R/C, 1649-038 Lisboa,Portugal

cEvodos, Weegbree 21, 4941 VT, Raamsdonksveer, the Netherlands

Low-shearcentrifuge(SpiralPlateTechnology,SPT,Evodos)Membraneunits

Pre-concentra*onofbiomassbymembraneprocessingpriortocentrifuga*on-  Reduceoverallenergyandcapitalcosts-  Maximisevolumetricfluxeswhilemaintainingcellintegrityatmaximisedvaluesof

concentraDonfactors

12.5%(5.43h)

0

10

20

1 5 9 13 17

%Lossofintegrity

CFoverall(-)

1stHarves6ng

2ndHarves6ng

3rdHarves6ng

“Green”D.salina “Orange”D.salina

0

15

30

1 9 17 25 33

%Lossofintegrity

CFoverall(-)

1stHarves0ng

2ndHarves0ng

3rdHarves0ng

0

20

40

1 5 9 13 17

Jv (L

/(m2 .

h))

1stHarves1ng

2ndHarves1ng

3rdHarves1ng

CFoverall=16.4(or94%permeaterecovery)Jvaverage=22L/(m2.h)

Cellintegrityloss=13%

CFoverall=36.7(or97%permeaterecovery)Jv=10L/(m2.h)

Cellintegrityloss=7%

2DFluorescenceSpectroscopy

λ em (nm)

250 300 350 400 450 500 550 600 650

λ ex

(nm

)

300

350

400

450

500

550

600

650

700

I II

III

I.  ProteinsII.  HumiccompoundsIII.  Pigments

0,0E+0

4,0E+5

8,0E+5

1,2E+6

0,0E+0 4,0E+5 8,0E+5 1,2E+6

CellCo

nc.(cells/m

L)

Observed

CellConc.(cells/mL)Predicted

2σ Train.:R2=0.86,Slope=1.00

Valid.:R2=0.83,Slope=0.78

RMSEP=1.3E+5cells/mL

Train.:R2=0.91,Slope=1.00


RMSEP=5.3%viability

60

70

80

90

100

110

60 70 80 90 100 110

Viability(%

)Observed

Viability(%)Predicted

0,0E+0

1,0E+7

2,0E+7

0,0E+0 1,0E+7 2,0E+7

CellsCon

c.(cells/mL)

Observed

CellConc.(cells/mL)Predicted



RMSEP=1.4E+6cells/mL



RMSEP=10%viability

20

40

60

80

100

20 70

Viability(%

)Observed

Viability(%)Predicted

Cellnumber

PrincipalComponentAnalysis(PCA)

ProjecDontoLatentStructures(PLS)

findcorrelaDonstopredictcellulargrowthandotherkeyperformanceparameters

§  Cellnumber§  Cellintegrity§  Glycerol§  Pigments§  Enzymes§  Proteins

300 400 500 600 700

300

400

500

600

700

X Axis Title

Y Ax

is Ti

tle

0

25.00

50.00

75.00

100.0

125.0

150.0

175.0

200.0



RMSEP=0.23absunits(at410nm)

0

1

2

3

4

0 1 2 3 4

UnitsofA

bs410nm

Observed

UnitsofAbs410nmPredicted

%Viability

ExtracellularGlycerol

91mg/L

262mg/L

137mg/L

0

100

200

300

0 1 2 3 4 5 6

Glycerolco

nten

t(mg/L)

Time(h)

UV+H2O2

Ozono+H2O2

0

25

50

0 1 2 3 4 5 6

Nitrites&

Nitra

tes(mg/L)

Time(h)

Ozono+H2O2

UV+H2O2

Permeaterecoveryformicroalgaecul*va*onmedia

Mediumrecycling

Maximumvolumetricproduc*vityofcarotenoids(mgCar/L/d)

Permeatewithouttreatment 6.93±0.48

Permeate+UV+H2O2 5.98±0.41

Evodos Dynamic settler

liters/hour Intactcellsharvested dryweightcontentEvodos10 250 Yes,>95% 20-30%Evodos10 300 Yes,>95% 20-30%

Max.capacity350liters/hour

Evodos50 harvest

•  Dunaliellaintactalgae•  40%solids•  highbeta-carotenetoabove10%

AFDW

Evodos50:-4000L/h-95%separaDonefficiency->20h/day

Conclusions

Evodos10 harvest

EconomicEvalua*onofHarves*ng“green”Dunaliellasalina

ü ReducDonofOPEX+CAPEXof52%

ü ReducDonofenergy

consumpDonof45%

ü Harves*ngof“green”DunaliellasalinabyultrafiltraDonallowedafinalCFof16.4andJv.averageof22L/(m2.h)withalossofintegrityof13%

ü Harves*ngof“orange”DunaliellasalinabyultrafiltraDonallowedanoverallCFof36.7andJvof10L/(m2.h)withalossofintegrityof7%

ü Reduc*onoftheOPEX+CAPEXof52%andreduc*onofenergyconsump*onof45%

ü FluorescencespectradisDnguishdifferencesinmicroalgaemedia.ModelforDunaliellasalinaculturespredictedcellconcentraDonandintegrity

ü UltrafiltraDonpermeatetreatedbyUV+H2O2allowsformediumrecyclingforDunaliellasalinaculture

ü SuccessfulharvesDngofDunaliellasalinawithEvodosSpiralPlateTechnology;intact/undamagedalgae,highsolidcontent,>95%separa*onefficiency

ü SuccessfuldevelopmentfromPilotEvodos10unittoindustrialautomatedEvodos50unit

Basedon:EnergyconsumpDon(membraneunit)=0.87kWh/m3

EnergyconsumpDon(centrifuge)=2.5kWh/m3

References[1]MonteJ.,etal,Sep.Pur.Technol.,2018,190,252,260[2]SáM.,elal,AlgalResearch,2017,24,325–332

0

10

20

1 9 17 25 33

Jv(L/(m

2 .h))

1stHarves0ng2ndHarves0ng3rdHarves0ng

introduction permeate recycling · introduction title text permeate recycling harvesting and...

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