introduction to dsmz, the german collection of ... · budapest treaty for bacteria, fungi, yeasts...
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INTRODUCTION TO DSMZ, THE GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES
Prokaryotes
Plant Cell Cultures
Plant Viruses and Antisera
Human and Animal Cell Cultures
TODAY, DSMZ IS A RENOWNED RESEARCH AND SERVICE INSTITUTION WITH COLLECTIONS OF
Yeasts and Fungi
• DSMZ originated from a purely internal research collection of theInstitute for Microbiology, University Göttingen
• 1969 founded as own department “Mikrobenbank“, later“Sammlung von Mikroorganismen Göttingen“ SMG, on projectfunding
COLLECTION OF BACTERIA, until 1969
SMG
1974 Establishment as DSM, a national service collection for thesupport of the development of national biotechnology ; concept of decentralized, specialized collections, housed in thematically related research institution; Göttingen, being thecentre with patent deposit and supply services, is institutionallyfunded.
Central Göttingen
aerobic endospore formingbacteria, anaerobic endospore and non-spore forming bacteria, photo-trophic bacteria, patent deposit
München
Gram-positive, non spore forming bacteria
Weihenstephan
Gram-negative Eubacteria
Bayreuth
Pseudomonadaceaeand marine bacteria
Berlin I
Phytopathogenicbacteria and fungi
Darmstadt
Actinomycetales
Berlin II
Filamentous fungi, yeasts
INSTITUTE FOR MICROBIOLOGY GÖTTINGEN
DSM
1976 – ‘78 centralization of 3 of the external bacterial collections; only strains, not staff (number of staff in Göttingen: 4 scientists, 8 technical laboratory assistants, 4 further technical assistants )
1979 transferred as independent department to GBF
1986 establishment of additional work groups (additional 5 scientists and 5 technical assistants); projectfunding
1987 move to Braunschweig into own new building and centralization of remaining two external bacterial groups(strains and staff)
organism groups covered by then:
More or less the whole breadth of bacterial and archaealdiversity, aerobic, anaerobic, extremophiles, ….; exceptrisk group 3 organisms;
smaller range of fungi and yeasts (because of CBS, NL; IMI =CABI Bioscience, UK in Europe)
NEW DSMZ BUILDING IN BRAUNSCHWEIG
● 1988 transformed into independent institution under publicfunding
● 1989 – ’90 enlargement by additional departments (animal cells, plant cells, plant viruses); on project funding
● since 1996, DSMZ, after nearly 30 years of instable funding , ‘Blue List‘ Institute, institutional funding, 50% federal government, 50% Länder
1991 Retirement of Director Dr. Dieter Claus
Since '93 New Director: Prof. Dr. Erko Stackebrandt
1974 renamed as DSM and recognized by the German patent office and the European Patent Office for microorganisms
1981 status as International Depositary Authority under the Budapest Treaty for bacteria, fungi, yeasts and bacteriophages
1988 status as IDA for isolated plasmid preparations
1990 incorporation of the IDA ZIMET, Jena
1990 status as IDA for plant viruses and plant cell cultures
1991 status as IDA for animal and human cell cultures
1994 status as IDA for murine embryos
HISTORY OF DSMZ with regard to its patent depositary
DSMZ - GeneralDSMZ - General
in all cases material not exceeding RG 2
DSMZ - GeneralDSMZ - General
STAFF TODAY
SCIENTIFIC STAFF
● 25 PHD LEVEL AND ABOVEBIOLOGISTS, MICROBIOLOGISTS, AGRICULTURAL AND MEDICAL SCIENTISTS
TECHNICAL GRADUATED STAFF
● LABORATORY~ 25 BTAS, CTAS, MTAS, LTAS
● OFFICE~ 14 SECRETARY, INTERNAL ORGANISATION, IMPORT-EXPORT SPECIALISTS, PUBLIC RELATION, COMPUTER SPECIALISTS
TECHNICAL NON GRADUATED STAFF~4 GENERAL HELP, CLEANING GLASS WARE
DIPLOMA AND PhD STUDENTS, POST DOCS, GUEST RESEARCHERS
● Collecting, maintaining and supplying biological diversity of microorganisms, cell cultures and plant viruses
● Research in collection related areas, publication activities, cooperation in research areas (e.g. as editors and reviewers in relevant journals, in ICSP, as consultants, etc.)
● Worldwide cooperation with other collections and in international organisations (e.g. WFCC, ECCO, CABRI, EBRCN, UNESCO, GBIF, EUROCAT, ENBI, OECD-BRC, etc.)
● DSMZ is the national GBIF Node for Prokaryotes and Viruses
MicrobiologyMicrobiology
STORAGE OF FREEZE-DRIED AMPOULES
LIQUID NITROGEN STORAGE
MicrobiologyMicrobiology
MANAGEMENT OF RESOURCES: CLOSE INTERLINKAGE OF RESEARCH AND SERVICE IN THE DEPARTMENT OF
MICROBIOLOGY
RECULTIVATION REPLENISHMENT OF STOCKS
NEW ACCESSIONS ENLARGERMENT OF THE COLLECTION
COLLECTION RELATED RESEARCH
IDENTIFICATION
AUTHENTICATION
MicroorganismsMicroorganisms
Phase ContrastMicroscope; standardmagnification: x40; x100
Dissecting Microscope; standard magnification: x0,8 – x2
VIEW OF LABORATORY
MicrobiologyMicrobiology
BIOCHEMICAL TEST SYSTEMSMicrobiologyMicrobiology
MicroorganismsMicroorganisms
INCUBATOR ROOM
MOLAR G+C CONTENT OF DNA
IdentificationIdentification
DNA – DNA HYBRIDIZATION
IdentificationIdentification
DNA ISOLATION
IdentificationIdentification
16S rRNA SEQUENZING
IdentificationIdentification
RIBOPRINTER
IdentificationIdentification
ONE AND TWO DIMENSIONAL TLC FOR CELL WALL ANALYSIS
IdentificationIdentification
DISPATCH DEPARTMENT
MicrobiologyMicrobiology
Quality Management at DSMZ
Implementation of an International Recognised Quality Standard
covering
Management of Expertise and Protection of Diversity:
DIN EN ISO 9001:2000
Expectations from and role in Demonstration Project
Expectations
- transfer in-house procedures into harmonized SOP
- fill gaps identified during comparison of in-house and OECD procedures (e.g. risk assessment)
- develop audit scheme for OECD guidelines alongISO
- develop training schemes
- using synergy effect for working towards a national network of BRCs
Role
- Dagmar Fritze funded for Demonstration Project central secretariat (BMBF, DSMZ)
- output from DSMZ projects (e.g. EMbaRC, training) or internal work provided to workpackages (e.g. lessons learnt from ISO 9000 implementation)
- international contacts (e.g. regional input from ECCO)
THANK YOU !