introduction to the light microscope
DESCRIPTION
TRANSCRIPT
![Page 1: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/1.jpg)
Introduction to the Light Microscope
![Page 2: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/2.jpg)
Parts of a binocular microscope
![Page 3: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/3.jpg)
Binocular body
Rotating Nose Piece (turret)
Stage clips for slidesObjective lenses
StageFine focus
Coarse focus
Condenser
Rheostat
Base
Y axisX axis
Stage adjustmentknobs
Light source
![Page 4: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/4.jpg)
Condenser
Aperture iris diaphragmin condenser
RheostatControls brightness
Light source
![Page 5: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/5.jpg)
Width control
Individual focus
Ocular Lenses
![Page 6: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/6.jpg)
Bright-Field Microscopy
• Image is produced when light is passed through specimen.
• Contrast is improved with staining techniques
![Page 7: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/7.jpg)
Image Production in a Compound Light Microscope
• Light from the source is focused on the specimen by the condenser lens.
• It then enters the objective lens, where it is magnified to produce a real image.
• The real image is magnified again by the ocular lens to produce a virtual image that is seen by the eye.
![Page 8: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/8.jpg)
Total Magnification
• The amount of magnification is marked on each lens
• Total Magnification = Objective X Ocular
10X
40X
10x X 40x = 400x
![Page 9: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/9.jpg)
Resolution• Resolution: clarity of image• Limit of resolution (resolving power): how far
apart two points must be for the microscope to view them as being separate
• Light microscope = 0.2 um
![Page 10: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/10.jpg)
Numerical Aperture
• NA = lens’s ability to “capture” light coming from specimen to make image
• Oil immersion increases NA, therefore increases its resolving power.
![Page 11: Introduction to the light microscope](https://reader033.vdocument.in/reader033/viewer/2022061120/546d88abaf7959e3298b5f21/html5/thumbnails/11.jpg)
• Hold cover slip at 45 degree angle• Slowly lower cover slip to prevent bubbles• (click on title above to view animation)
Making a Wet Mount