isolation of rare circulating tumor cells
DESCRIPTION
Isolation of Rare Circulating Tumor Cells. Sunitha Nagrath et al. Presentation by: Keara Darragh , Matthew Faw , and Will Plautz. http://www.nibib.nih.gov/news-events/newsroom/circulating-tumor-cells-captured-last. Overview of Metastasis and CTCs. Circulating tumor cells (CTCs) - PowerPoint PPT PresentationTRANSCRIPT
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Isolation of Rare Circulating Tumor Cells
Sunitha Nagrath et al.
Presentation by: Keara Darragh, Matthew Faw, and Will Plautz
![Page 2: Isolation of Rare Circulating Tumor Cells](https://reader033.vdocument.in/reader033/viewer/2022061519/56816631550346895dd999fe/html5/thumbnails/2.jpg)
Overview of Metastasis and CTCs
• Circulating tumor cells (CTCs)• Likely the origin of intractable
metastatic disease• 1 CTC : 10^9 haematologic cells in the
blood of cancer patients
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CTC chip better than existing methods
• Gross prognostic tool (high/low risk)
• Isolates fewer CTCs with very low purity (<1%) and yield
• Multiple bulk semi-automated preparatory steps
• First microfluidic device that can successfully isolate viable cells from milliliter volumes of whole blood• High sensitivity, selectivity, and yield• Single step
Circulating TumorCell (CTC) Chip
Immunomagnetic-bead purification
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What is Microfluidics? • Deals with small volumes (fL, pL, nL, µL)
of fluids• Generally processed in a way that
moves, mixes, or separates the contents• Microfluids act differently
• Surface tension and low Reynolds number become larger factors in the system
• Different types of microfluidics• Dealing with continuous-flow today
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What is Microfluidics? • Deals with small volumes (fL, pL, nL, µL)
of fluids• Generally processed in a way that
moves, mixes, or separates the contents• Microfluids act differently
• Surface tension and low Reynolds number become larger factors in the system
• Different types of microfluidics• Dealing with continuous-flow today
![Page 6: Isolation of Rare Circulating Tumor Cells](https://reader033.vdocument.in/reader033/viewer/2022061519/56816631550346895dd999fe/html5/thumbnails/6.jpg)
What is Microfluidics? • Deals with small volumes (fL, pL, nL, µL)
of fluids• Generally processed in a way that
moves, mixes, or separates the contents• Microfluids act differently
• Surface tension and low Reynolds number become larger factors in the system
• Different types of microfluidics• Dealing with continuous-flow today
![Page 7: Isolation of Rare Circulating Tumor Cells](https://reader033.vdocument.in/reader033/viewer/2022061519/56816631550346895dd999fe/html5/thumbnails/7.jpg)
How Does the Chip Capture
Circulating Tumor Cells?• 25 mm x 66 mm
silicon chip • 78,000 microposts• epCAM antibodies
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CTC Isolating Microfluidic Device
25mm
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Staining to Assess Purity
• Stains are used in order to identify malignant cells from healthy cells.
• Malignant cells look different• Large cellular size• Relatively large nuclei• Visible nucleoli
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Results and Applications
• Successfully identified CTCs in 115/116 samples• Approximately 50% purity• CTCs isolated (per ml):
• NSCLC: 5-1,281 • Breast: 5-174
• Monitor cancer treatment response
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Results and Applications
• Successfully identified CTCs in 115/116 samples• Approximately 50% purity• CTCs isolated (per ml):
• NSCLC: 5-1,281 • Breast: 5-174
• Monitor cancer treatment response
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Conclusion• First microfluidic device that can successfully process milliliter volumes of whole blood• High sensitivity, selectivity, and yield• Single step• Isolation of viable cells• Allows for real time monitoring of cancer progression in a non-invasive manner