isopenicillin n synthase – proposed mechanism
DESCRIPTION
Isopenicillin N Synthase – Proposed Mechanism. Trapped in year 2. Spectroscopic Properties of the C-H Cleaving Intermediates. Fe(IV)-Oxo. Fe(III)- Superoxo. Velocity (mm/ s ). Trapped in year 1. - PowerPoint PPT PresentationTRANSCRIPT
HNS
H COOH
HN
AA
O
OH
HFe2+
HisAsp
His
H2O
GlnFe2+
HisAsp
His
H2O
OH2 + ACV + O2HN
S
HCOOH
HN
AAO
OH
HFe3+
HisAsp
His
H2OO O
NS
HCOOH
HN
AAO
OH
Fe2+
HisAsp
His
H2OO OH
HNS
HCOOH
HN
AAO
OH
Fe3+
HisAsp
His
H2OO OH
HNS
HCOOH
HN
AAO
OH
Fe4+
HisAsp
His
H2O O
NS
COOH
HN
AAO
OH
Fe3+
HisAsp
His
H2O OH
NS
COOH
HN
AAO
OH
Fe2+
HisAsp
His
H2OOH
- IPNA B C
D
EFG
H
- H2O
- H2O
Isopenicillin N Synthase – Proposed Mechanism
Trapped in year 2
Trapped in year 1
0
0.06
0.1
300 400 500 600 700
2H-ACV zeroed
2.5ms20ms100ms200ms2s
Wavelength (nm)
Absorbance
Fe(IV)-Oxo
Fe(III)-Superoxo
Velocity (mm/s)
Spectroscopic Properties of the C-H Cleaving Intermediates