Please refer disclaimer Overleaf.

ITC Broth Base (TTC Broth Base) M1220

Composition**Ingredients Gms / LitreTryptone 10.000Yeast extract 1.000Magnesium chloride hexahydrate 60.000Sodium chloride 5.000Malachite green 0.010Triclosan (Irgasan) 0.001Final pH ( at 25°C) 6.9±0.2**Formula adjusted, standardized to suit performance parameters

DirectionsSuspend 44.11 grams (the equivalent weight of dehydrated medium per litre)in 988 ml purified / distilled water. Heat to boiling to dissolve the medium completely.Sterilize by autoclaving at 15lbs pressure(121°C)for 15 minutes. Cool to 45-50°C. Aseptically add rehydrated contents of 1 vial of Ticarcillin Supplement (FD102) and Potassium Chlorate Supplement (FD103). Mix well before dispensing in sterile tubes or flasks as desired.

Principle And Interpretation

The genus Yersinia belongs to the family Enterobacteriaceae. They are usually nitrate reductase positive and show fermentative metabolism. The genus comprises of 11 species, of which Yersinia enterocolitica is most important as a causative agent of human foodborne illness. Variety of enrichment methods has been described for recovery of Y.enterocolitica from foods. The most efficient procedures for recovering enteropathogenic bacteria from foods have incorporated at least one and often two enrichment steps before plating onto selective differential agar media. ITC Broth is formulated in accordance with APHA (7) and is recommended by ISO Committee (3) as a selective enrichment medium for Y.enterocolitica from foods. ITC Broth was developed by Wauters et al (8) as a new enrichment broth, derived from modified Rappaport Broth and based on the selective agents irgasan, ticarcillin and potassium chlorate.

Tryptone and yeast extract provide nitrogeneous and carbonaceous compounds, long chain amino acids and other essential growth nutrients. Ticarcillin has inhibitory action on both gram-positive and gram-negative organisms. Irgasan inhibits gram-positive organisms. Potassium chlorate has disinfecting properties.For enrichment prepare 1: 10 homogenate of food sample by weighing 25 grams of food and adding it to 225 ml of primary enrichment medium. Prepare homogenate and carefully transfer the homogenate into sterile jar for incubation. After incubation, streak onto agar plates such as MacConkey Agar (M081). After incubation, observe for the colonies of Yersinia, which are pinkish coloured, smooth and have an entire edge. Colonies of Yersinia are larger on agar media when incubated at 25°C as Y.enterocolitica is more active biochemically at 25°C than at 35-37°C.

Intended Use:Recommended for selective enrichment and enumeration of Yersinia enterocolitica.

Type of specimen Clinical samples - Faeces; Food and dairy samples; Water samples

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,6,9). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(2) After use, contaminated materials must be sterilized by autoclaving before discarding.

Specimen Collection and Handling:

HiMedia Laboratories Technical Data

6.70-7.10Cultural ResponseCultural characteristics observed with added Ticarcillin Supplement(FD102) and Potassium Chlorate Supplement (FD103) after an incubation at 25-30°C for 24-48 hours.Organism Inoculum

(CFU)Growth

Escherichia coli ATCC25922 (00013*)

>=104 inhibited

Staphylococcus aureus subsp. aureus ATCC 25923 (00034*)

>=104 inhibited

Yersinia enterocoliticaATCC 27729

50-100 good-luxuriant

Quality ControlAppearanceLight yellow to light blue homogeneous free flowing powderColour and Clarity of prepared mediumPeacock green coloured, clear solution without any precipitateReactionReaction of 4.41% w/v aqueous solution at 25°C. pH : 6.9±0.2pH

Warning and Precautions :In Vitro diagnostic Use . Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Please refer disclaimer Overleaf.

expiry period Performance and EvaluationPerformance of the medium is expected when used as per the direction on the label within thewhen stored at recommended temperature.

Key : *Corresponding WDCM numbers.

Storage and Shelf Life Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).

Disposal

1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C.2. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater, 23rded., APHA, Washington, D.C.3. International Organization for Standardization (ISO), 1994, Draft ISO/DIS 10273.4. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.5. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual ofClinical Microbiology, 11th Edition. Vol. 1.

Reference

6. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed.,American Public Health Association, Washington, D.C.

Disclaimer :

User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: techhelp@himedialabs.com Website: www.himedialabs.com

HiMedia Laboratories Technical Data

7. Vanderzant C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination of Foods, 3rd Ed., APHA, Washington, D.C.

8. Wauters G., Goossens V., Janssens M. and Vandepitte J., 1988, In. J. Syst. Bacteriol., 38, 424-429.9. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th

Ed., APHA Inc., Washington, D.C.

Revision : 03 / 2019

In vitro diagnostic medical

device

CE Marking

Do not use if package is damaged

CE Partner 4U ,Esdoornlaan 13, 3951

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IVD

Storage temperature

10°C

30°C

EC REP

HiMedia Laboratories Pvt. Limited, 23 Vadhani Industrial Estate, LBS Marg,Mumbai-86,MS,India