ITR 5’ ITR 3’

ORF codant la transposase (Tnp)

Hsmar1:

miHsmar1 (80 pb)

b.

Position TA locationin ccdB1170-tggctgtgTATCAGG/clone1/CCTGATAtaagggag (ccdB 3’UTR)1189-ctgacattTATCAGG/clone2/CCTGATAtattcccc (ccdB stop codon)1211-catcaggtTATCAGG/clone3/CCTGATAatggcgtt (ccdB ORF)1471-ctcttttaTATCAGG/clone4/CCTGATAggtgtaaa (ccdB ORF)1477-tataggtgTATCAGG/clone5/CCTGATAaaccttaa (ccdB ORF)

ccdB+

KanaR

pUC ori

a.

ccdB+

KanaR

pUC oripUC ori

AmpR

lacZ’

mini-Mos1pDONR221-CmR- ITR3p

A

Random Integrations in the pDONR221-CmR-

Selection of the transposition events in ccdB- by tranfecting in DH5 bacteria and

plating on LB-kanamycin petri dishes

a.1

8711357

18 13074

35 14791

52 164108

70 182126

87 199143

123456

d.3’ITR (TA)TCAGGTGTACAAGTATGAAATGTCGTTT5’ITR (TA)cCAGGTGTACAAGTAgGgAATGTCGgTT 104 107

b.1 2 3 4 5 6

Free ITR

Complexes

Probes

0.100.120.140.160.180.200.22

50 70 90 110 130 150Probe Center

Rfc.

TA 5’5’ *

Non Transferred strand Transferred strandTA 5’

5’

*

T

ATCAGGTGTACAAGTATGAAATGTCGTT

tcT

gga

tgatc

g

C

TAGTCCA

ATGTTCATACTTT

ACA

GCAAA

agA

cct

actag

ct

G+A

PEC1

SEC2

G+A

PEC1

SEC2

5’ gaactagtggatc TA TCAGGTGTACAAGTATGAAATGTCGTTTgatc 3’ (NT)3’ cttgatcacctag AT AGTCCACATGTTCATACTTTACAGCAAActag 5’ (T)

a.

b.

+

5’ nnnTAT+1C+2A+3GGTGT… 3’ (NT)3’ nnnATA+1G+2T+3CCACA… 5’ (T)

+ + + +Mos1

5’ nnnTAA+1C+2A+3GGTTG… 3’ (NT)3’ nnnATT+1G+2T+3CCAAC… 5’ (T)

*

* * * *Himar1

5’ nnnTAT+1T+2A+3GGTTG… 3’ (NT)3’ nnnATA+1A+2T+3CCAAC… 5’ (T)

* * *

* * *Hsmar1

d.

a.

b.

c.

Mobilité des Packs miHsmar1

Question caduque

Evolution du projet dans Modulome

Fabriquer un outil pour inventorier les miHsmar1 dans

les génomes de primates