it’s more than just colors, my seminar

“It’s more than just colors!”

Finding novel uses for natural dyes from Philippine indigenous plants

Therese Julienne T. MedinaMS MBB

http://farm3.static.flickr.com

• Introduction– Natural Dyes: A Definition– History of natural dyes and dyeing– Classic examples of dye-yielding plants– Traditional uses of natural dyes– The Rise of Synthetic Dyes: Implications– Ideals of the 21st century: “Going Natural”– Recent Studies: Novel uses of Natural Dyes

• In the Philippine Context: A Proposal– Natural Dyes in the Philippines: The New

Challenge– Philippine Indigenous Dye-yielding Plants– Natural Dyes R&D: Rediscovering dye-yielding

plants• Anatomical and Histochemical Procedures• Extraction and Constituents Identification• Bioassay studies for therapeutic potential• Elaboration of natural dye production: role of

biotechnology

Natural Dyes: A definition

• COLOR - a molecule that absorbs certain wavelengths of visible light and transmits or reflects others.

• COLORANT - the activity of the molecule when applied to a substance.

• DYES - materials that gives color to objects such as yarn, food, paper, cosmetics, and cloth.

• NATURAL DYES/ BIOCOLORANTS - coloring agents obtained from nature or biological sources: plants, animals, fungi, and bacteria.

Natural Dyes: A definition

History of natural dyes and dyeing

http://traditions.cultural-china.com/en/15Traditions1024.html

http://indusvalleycivilizationf.blogspot.com/2010/03/rilli-quilt-ancient-handicraft-of-indus.html

www.ancientresource.com/lots/egyptian.html

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china- silk with color (2600BC)indus valley (2500)egypt- mummy (tut)mayan & aztecget pics!!!

History of natural dyes and dyeing

http://www.immortalitea.com/Mulberry_history.htm www.dawns-light.co.kr/en/page11.htmlhttp://northstoke.blogspot.com/2009/11/indigo-dyeing.html

flickr.com/photos/35237098989@N01/22767364/

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process of dyeing: 1. extraction of colouring matter- powder material, boil in water for 10-20 mins, yarn or fabric is washed thoroughly then heated in extract at different temps for 30-40 mins2. creating a bong between the colouring matter and material to be dyed (mordsnting: metallic salts)3. actual dyeing (depends on the material to be dyed)get Picture of the dyeing process

Classic examples of dye-yielding plants

Rubia tinctorum

http://www.fibrecrafts.com/resource/fact_file/dyes/fibrecrafts_natural_dyes.asp

Indigofera tinctoria


http://www.fibrecrafts.com/resource/fact_file/dyes/fibrecrafts_natural_dyes.asp

Anthemis tinctoria


:www.zelenhoz.com/n/f4.htmhttp://www.fibrecrafts.com/resource/fact_file/dyes/fibrecrafts_natural_dyes.asp

Quercus infectoria


www.elizabethancostume.nethttp://www.fibrecrafts.com/resource/fact_file/dyes/fibrecrafts_natural_dyes.asp

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picture!!!!

Traditional uses of natural dyes

http://www.sagegrovecoven.com www.himfr.com/d-p114221535560486025-Natural_Dyes/

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textiles: wool, cotton, and thread dyeingfood color - japanese traditional dish with colorcosmetics: egyptians (eyeliner!)get pics

The Rise of Synthetic Dyes: Implications

William Henry Perkin• Accidentally produced and

discovered MAUVE, the first synthetic dye

• More synthetic dyes were created after his discovery

• Natural dye use steadily declined

http://www.soci.org/About-Us/About-SCI/History/Notable-Chemists

The Rise of Synthetic Dyes: Implications

http://ecologicalartist.wordpress.com/2009/07/http://goldencarla.typepad.com/carla_goldens_get_healthi/golden-spectrum/

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pictures: effect on the environment of using dyes-- waste water being dumped on bodies of waterin food industry: artificial food colorings that are carcinogenicsget pictures!!!!

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This is a picture of a stagnant pond outside of a textile factory in Bangladesh, where dye runoff filled with a panoply of heavy metals and petroleum by-products sit, untreated.

Ideals of the 21st century:

“Going Natural”

ters

get icon for 'go natural'people are becoming aware of the harmful effects of synthetic dyes, both on the environment and health of the consumers.hence, natural dye production is being revived

• Natural dyes as natural food grade biocolorants

Recent Studies: Novel uses of Natural Dyes

• Natural dyes in food industry: as food preservatives, quality control markers, and nutritional supplements


anthocyanins

carotenoids

riboflavin

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anthocyanin can be anti bacterialcarotenoids can act as sun screen, maintaining the quality of the foodanthocyanin profiles of jams can be used to assess the quality of the product by identifying the kind of fruit that was used to make the jamcarotenoids, beta-xanthin, and riboflavin are food grade biocolorants that also adds nutritional value to the food

• Natural dyes as therapeutic agents



• Natural dyes as photosensitizers for dye-sensitized solar cell

Hao et.al., 2005. Natural dyes as photosensitizers for dye-sensitized solar cell

In the Philippine Context: A Proposal

Natural Dyes in the Philippines:The New Challenge

• Revival of natural dye industries– Abra and Ifugao, re-establish plantations of dye-

yielding trees – Natural dye R&D Program

• CHALLENGE: establish new applications and techniques for the improvement of man’s health and wellness

Philippine Indigenous Dye-yielding Plants

• Indigofera tinctora, Terminalia catappa, Curcuma longa, Bixa orellana, Caesalpinia sappan

http://www.djibnet.com/photo/fabaceae/indigofera-tinctoria-1655002816.html http://fr.academic.ru/dic.nsf/frwiki/1614018

http://otvet.mail.ru/question/29923774/

• Acacia farnesiana, Michelia chanpaca, Hibiscus sabdariffa, Leucaena leucocephala, Mirabilis jalapa

Philippine Indigenous Dye-yielding Plants

http://www.desertfolia.com/index.php?mact=News,cntnt01,detail,0&cntnt01articleid=5&cntnt01returnid=67

www.jungleseeds.com/SeedShop/TropicalFruit.htmwww.backyardnature.net/yucatan/bean-fam.htmwww.natureproducts.net/.../Mirabilis.html

Natural Dyes R&D: Rediscovering dye-yielding plants

• Anatomical and Histochemical Procedures

http://www.indianjournals.com/ijor.aspx?target=ijor:apps&volume=15&issue=1&article=040http://www.flickr.com/photos/nikyhughes/395720897/

• Extraction and Constituents Identification


http://www.biomedcentral.com/1472-6750/8/25/figure/F6?highres=yhttp://www.knaes.affrc.go.jp/sporf/no14/m4.html


• Extraction and Constituents Identification

Color Generic name (chemical group)

Examples

Blue and Purple Dyes

IndicanAnthocyanins

IndigoCyanidin

Red dyes AnthraquinoneNeoflavonoids

AlizarinBrazilin

Yellow Dyes FlavonesFlavonol

LuteotinMorin

Black and Brown Dyes

Tanins Gallic acid Catechin

• Bioassay studies for therapeutic potential


www.fgsc.net/fgn39/ciufet.html

Microbial assay for antimicrobial effect against:Escherichia coliBacillus subtilisKlebsiella pneumoniaeProteus vulgarisPseudomonas aeruginosa

• Elaboration of natural dye production: role of biotechnology


http://www.ars-fla.com/Mainpages/Bio-Reactor/bioreactor.html

SUMMARY• Natural dyes:

– Were used thousands of years ago for textiles, foods, and cosmetics

– Were replaced by synthetic dyes in the market (because of their cheaper cost)

– are being revived and studied for new applications (biocolorant, anti-microbial agent, photosensitizer)

– In the Philippines, R&D programs on natural dyes are established.

There is a need for:

• proper collection,• documentation,• assessment and characterization of dye

yielding plants and their dyes, and• research to overcome the limitations of

natural dyes

References• Abello, M. 2007. Of indigo, talisay, and natural dying technology. Agriculture magazine.

11(12):26-27.• Chattopadhyay, P., S. Chatterjee, and SK. Sen. 2008. Biotechnological potential of natural

food grade biocolorants. African Journal of Biotechnology. 7(17):2972-2985. • Gokhale, SB., AU. Tatiya, SR. Bakliwal, and RA. Fursule. 2004. Natural dye yielding plants in

India. Natural Product Radiance. 3(4):228-234.• Quinto, J.E. 1992. Anatomical Study of Some Dye Containing Species of Family Verbenaceae

and Stability of their Dyes. Unpublished masters’ thesis, UPLB.• Rosenberg, E. 2008. Characterization of historical organic dyestuffs by liquid

chromatography- mass spectrometry. Anal Bioanal Chem 391:33-57.• Singh R., A. Jain, S. Panwar, D. Gupta, and SK. Khare. 2005.Dyes and Pigments. 66(2):99-

102.• Siva, R. 2007. Status of natural dyes and dye yielding plants in India. Current Science. 92(7):

916-925

http://www.plazanimal.cl/cms/index.php

http://www.ars-fla.com/Mainpages/Bio-Reactor/bioreactor.htmlhttp://www.ars-fla.com/Mainpages/Bio-Reactor/bioreactor.htmlhttp://www.knaes.affrc.go.jp/sporf/no14/m4.htmlhttp://www.biomedcentral.com/1472-6750/8/25/figure/F6?highres=yhttp://www.indianjournals.com/ijor.aspx?target=ijor:apps&volume=15&issue=1&article=040http://www.flickr.com/photos/nikyhughes/395720897/

http://www.desertfolia.com/index.php?mact=News,cntnt01,detail,0&cntnt01articleid=5&cntnt01returnid=67www.jungleseeds.com/SeedShop/TropicalFruit.htmwww.backyardnature.net/yucatan/bean-fam.htmwww.natureproducts.net/.../Mirabilis.html

http://www.djibnet.com/photo/fabaceae/indigofera-tinctoria-1655002816.htmlhttp://fr.academic.ru/dic.nsf/frwiki/1614018

http://otvet.mail.ru/question/29923774/

http://traditions.cultural-china.com/en/15Traditions1024.html

http://indusvalleycivilizationf.blogspot.com/2010/03/rilli-quilt-ancient-handicraft-of-indus.htmlwww.ancientresource.com/lots/egyptian.html

Thank You!

it’s more than just colors, my seminar

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