CURRICULUM VITAE DR MIRELLA BELLERI

Persona data

Born on August 21 st 1967 in Brescia ltaly ltalian citizenship Work address Unit of Experimental Oncology and lmmunology Department of Molecular and Translational Medicine University of Brescia Viale Europa 11 Phone number +39 030 3717310 E-mail mirellabelleriunibsit

Education

1986 degree in Biologica and Chimica Laboratory Technician at Istituto Professionale Camilla Golgi (6060) 1994 University Bachelor in Biomedica Laboratory Technician (110110 cum laude) 2011 University Master in Biomedica Laboratory Technician (11011 Ocum laude)

Research experience

1986-1990 fellowships from the Lega Italiana per la Lotta contro i Tumori Laboratory Technician at the University of Brescia Cellular biology and screening of anti-angiogenic compounds

1991-present Laboratory Technician at the University of Brescia angiogenesis and tumorigenesis assays

Technical skills and competences

Cellular Bioloqy culture of immortalized and prlmary cells ce ll proliferation migration invasion and morphogenesis assays Cel i culture transfection infectlon cellular subpopulation selection and study of gene expression

Angiogenesis assays in vitro (spherold sproutigraveng morphogenesis on matrigel fibrin gel collagen gel) ex vivo (aorta ring assay CAM) and in vivo (murine sc Matrigel and tumorigenesis assays) Screening of anti-angiogenic compounds

Microscopy immunocytochemistry immunofluorescence and time lapse microscopy Biochemistrv production and purification of recombinant proteins protein extraction silver stain western blot ELISA Molecular Biology DNA and RNA extraction PCR Animai handling Breeding of colonies of mice Genotyping WT heterozygous and Twitcher pups

Care and health of animals reared and housed Execution and evaluation of in vivo experiments (tumorigenesis assays pharmacological treatments)

Publications

1 REZZOLA S CORSINI M CHIODELLI P CANCARINI A NAWAZ IM COL TRINI D MITOLA S RONCA R BELLERI M LISTA L RUSCIANO D DE ROSA M PAVONE V SEMERARO F PRESTA M lnflammation and N-formyl peptide receptors mediate the anglogenic actigravevity of human vltreous humour in proliferative dlabetic retlnopathy DIABETOLOGIA 2017 Apr60(4)719-728 doi 101007s00125-016-4204-0 Epub 2017 Jan 13

2 BELLERI M PRESTA M Endothelial celi dysfunction In globoid cell leukodystrophy J NEUROSCI RES 2016 Nov94(11)1359-67 doi 101002jnr23744 Epub 2016 Apr 1 Revlew

3 GIACOMINI A ACKERMANN M BELLERI M COL TRINI D NICO B RIBATTI D KONERDING MA PRESTA M RIGHI M Brain angigraveoarchigravetecture and intussusceptlve microvascular growth in a murine model of Krabbe disease ANGIOGENESIS 2015 Oct18(4)499-510 doi 1O1007s10456-01 5-9481-6 Epub 2015 Aug 27

4 DAL MONTE M REZZOLA S CAMMALLERI M BELLERI M LOCRI F MORBIDELLI L CORSINI M PAGANINI G SEMERARO F ccedilANCARINI A RUSCIANO D PRESTA M BAGNOLI P Antianglogenic Effectiveness of the Urokinase Receptor-Derived Peptide UPARANT in a Model of Oxygen-lnduced Retlnopathy INVEST OPHTHALMOL VIS SCI 2015 Apr56(4)2392-407 doi 1O1167iovs14shy16323

5 REZZOLA S DAL MONTE M BELLERI M BUGATTI A CH IODELLI P CORSINI M CAMMALLERI M CANCARINI A MORBIDELLI L ORESTE P BAGNOLI P SEMERARO F PRESTA M Therapeutic Potential of Anti-Angiogenlc Multitarget N0-Sulfated E Coli K5 Polysaccharide in Diabetic RetlnopathyDIABETES 2015 Jul64(7)2581 -92 doi102337db14-1378 Epub 2015 Feb 18

6 REZZOLA S BELLERI M GARIANO G RIBATTI D COSTAGLIOLA C SEMERARO F PRESTA M In vitro and ex vivo retina ang iogenesis assays ANGIOGENESIS 2014 Jul17 (3)429-42 Review

7 ZIZIOLI D GUARIENTI M TOBIA C GARIANO G BORSANI G BRESC IANI R RONCA R GIACOPUZZI E PRETI A GAUDENZI G BELLERI M DI SALLE E FABRIAS G CASAS J RIBATTI D MONTI E PRESTA M Biochim Biophys Acta 2014 Apr1842(4)665-75 doi 101016jbbadis 201401008 Epub 2014 Jan 24

8 BELLERI M RONCA R COLTRINI D NICO B RIBATTI D POLIANI PL GIACOMINI A ALESSI P MARCHESINI S SANTOS MB BONGARZONE ER PRESTA M lnhibition of angiogenesis by 13-galactosylceramidase deficiency in globoid celi leukodystrophy BRAIN 2013 Sep136(Pt 9)2859-75

9 REZZOLA S BELLERI M RIBATTI D COSTAGLIOLA C PRESTA M SEMERARO F A nove ex vivo murine retina angiogenesis (EMRA) assay EXP EYE RES (2013) Jul 112 p 51-6

1O RONCA R ALESSI P COL TRINI D DI SALLE E GIACOMINI A LEALI D CORSINI M BELLERI M TOBIA C GARLANDA C BONOMI E TARDANICO R VERMI W PRESTA M Long pentraxin-3 as an epithelial-stromal fibroblast growth factor-targetigraveng inhibitor in prostate cancer J PATHOL (2013) Jun 230(2) p 228-38

11 COL TRINI O DI SALLE E RONCA R BELLERI M TESTI NI C PRESTA M Matrlgel plug assay evaluatlon of angiogenic response by reverse transcrlptlon-quantitatlve PCR ANGIOGENESIS (2013) Apr 16 (2) p 469-77

12 VARINSKA L VAN WIJHE M BELLERI M MITOLA S PERJESI P PRESTA M KOOLWIJK P IVANOVA L MOJZIS J Antiangiogenic activity of the flavonoid precursor 4-hydroxychalcone EUR J PHARMACOL (2012) Sep 15 vol691 (1-3) p 125-33

13 URBINATI C RAVELLI C TANGHETTI E BELLERI M GIACOPUZZI E MONTI E PRESTA M RUSNATI M Substrate-fmmobilized HIV-1 Tat drives VEGFR2a(v)p(3)-integrin complex formation and polarization in endothelial cells ARTERIOSCLER THROMB VASC BIOL (2012) May voi 32(5) p25-34

14 LIEKENS S BRONCKAERS A BELLERI M BUGATTI A SIENAERT R RIBATTI D NICO B GIGANTE A CASANOVA E OPDENAKKER G PEacuteREZ-PEacuteREZ MJ BALZARINI J PRESTA M The thymidine phosphorllase inhibltor 5 0-trityllnosine (KIN59) igraves an antianglogenc multilarget flbroblast groth factor-2 antagonist M MOL CANCER THER (2012) Apr voi 11 (4) p817-29

15 LEALI D INFORZATO A RONCA R BIANCHI R BELLERI M COLTRINI O DI SALLE E SIRONI M NORATA GO BOTTAZZI B GARLANDA G DAY AJ PRESTA M Long Pentraxin 3tltmor necrosis factor-stimulated gene-6 interaction a biologica rheostat for fibroblast growth factor-2 mediateci angiogenesis ARTERIOSCLER THROMB VASC BIOL (2012) Mar voi 32 (3) p 696-703

16 YEH JC CINDROVA-DAVIES T BELLERI M MORBIDELLI L MILLER N CHO CW CHAN K WANG YT LUO GA ZICHE M PRESTA M CHARNOCK-JONES DS FAN TP The natural compound n-butylidenephthalide derived from the volatigravele oil of Radix Angelica sinensis inhlblts angigraveogenesigraves In vitro and in vivo ANGIOGENESIS (2011) voi 14(2)187-97

17 RONCA R BENZONI P LEALI D URBINATI C BELLERI M CORSINI M ALESSI P COLTRINI D CALZA S PRESTA M DELLERA P Antlanglogenlc actlvlty of a neutralizing human single-chain antibody fragment against fibroblast growth factor receptor 1 MOLECULAR CANCER THERAPY (2010) voi 9 (12) 3244-53

18 VANDERCAPPELLEN J LIEKENS S BRONCKAERS A NOPPEN S RONSSE I OILLEN C BELLERI M MITOLA S PROOST P PRESTA M STRUYF S VAN DAMME J The COOH-Terminal Peptide of Platelet Factor-4 Variant (CXCL4L 1PF-4var47-70) Strongly lnhiblts Angiogenesis and Suppresses B16 Melanoma Growth In vivo MOLECULAR CANCER RESEARCH (2010) p 322-334 ISSN 1541 -7786

19 COL TRINI D RONCA R BELLERI M ZARDI L INDRACCOLO S SCARLATO V GIAVAZZI R PRESTA M lmpact ofVEGF-dependent tumour mlcro-envlronment on EDB fibronectin expression by subcutaneous human tumour xenografts in nude mice JOURNAL OF PATHOLOGY (2009) voi 219 p 455-462

20 ANDREacuteS G LEALI D MTOLA S COL TRINI D CAMOZZI M CORSINI M BELLERI M HIRSCH E SCHWENDENER R CHRISTOfORI G ALCAMI A PRESTA M A Pro-inflammatory Signature Mediates FGF2-induced Angiogenesis JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2009) p 2083-2108

21 BELLERI M RIBATTI D SAVIO M STIVALA LA FORTI L TANGHETTI E ALESSI P COL TRINI D BUGATTI A MITOLA S NICOLI S VANNINI V PRESTA M alphavbeta3 lntegrln-dependent antiangiogenic activity of resveratrol stereoisomers MOLECULAR CANCER THERAPEUTICS (2008) voi 7 p 3761-3770

22 MITOLA S MORONI E RAVELLI C ANDRES G BELLERI M PRESTA M Angiopoietin-1 mediates the pro-angiogenic activity of the bene morphogenlc protein antagonist Drm BLOOD (2008) voi 112(4) p 1154-11 57

23 STABILE H MITOLA S MORONI E BELLERI M NICOL S COL TRINI D PERI F PESSI A ORSATTI L TALAMO F CASTRONOVO V WAL TREGNY D COTELLI F RIBATTI D PRESTA M Bene morphogenlc proteln antagonist Drmgremlin is a novel proangiogenic factor BLOOD (2007) voi 109(5) p 1834-1840

24 MITOLA S BELLERI M URBINATI e COL TRINI D SPARATORE B PEDRAZZI M MELLONI E ANO PRESTA M Cutting edge extracellular hlgh mobility group box-1 proteln ls a proangiogenic cytoklne JOURNAL OF IMMUNOLOGY (2006) voi 176 p 12-15

25 BELLERI M RIBATTI D NICOLI S COTELLI F FORTI L VANNINI V STIVALA LA PRESTA M Antiangiogenlc and vascular targetlng activlty of the microtubule-destablllzlng transresveratrol derivative 354-trlmethoxystilbene MOLECULAR PHARMACOLOGY (2005) voi 67 p 1451-1459

26 PISANO C AULICINO C VESCI L CASU B NAGGI A TORRI G RIBATTI O BELLERI M RUSNATI M PRESTA M Undersulfated low-molecular-weight glycol-spllt heparln as an antlangiogenic VEGF antagonlst GL YCOBJOLOGY (2005) 15 1C-6C

27 PRESTA M ORESTE P ZOPPETTI G BELLERI M TANGHETTI E LEALI D URBINATI C BUGATTI A RONCA R NICOL S MORONI E STABILE H CAMOZZl M HERNANDEZ GA MITOLA S DELLERA P RUSNATI M RIBATTI D 1Antianglogenlc Activlty of Semisynthetic Biotechnological Heparins Low-Molecular-Weight-Sulfated Escherichia colf K5 Polysaccharide Derlvatlves as Fibroblast Growth Factor Antagonists ARTERIOSCLER THROMB VASC BIOL (2005) 25 71-76

28 CASU B GUERRINI M GUGLIERI S NAGGI A PEREZ M TORRI G CASSINELLI G RIBATTI D CARMINATI P GIANNINI G PENCO S PISANO C BELLERI M RUSNATI M and PRESTA M Undersulfated and glycol-split heparins endowed with antlangiogenic activigravety J MEO CHEM (2004) 47 838-848

29 CASU B GUERRINI M NAGGI A PEREZ M TORRI G RIBATTI D CARMINATI P GIANNINI G PENCO S PISANO C BELLERI M RUSNATI M PRESTA M Short heparin sequences spaced by gllcol-split urinate resldues are antagonigravest of Fibroblast Growth Factor-2 and anglogenesis inhibitors BIOCHEMISTRY (2002) 41 10519-10528

30 PRESTA M BELLERI M VACCA A and RIBATTI D Antl-anglogenic activity of the purine analog 6-th loguanine LEUKEMIA (2002) 16 1490-1499

31 SCAPINI P NESI L MORINI M TANGHETTI E BELLERI M NOONAN D PRESTA M ALBINI A CASSATELLA MA Generation of Biologically Actlve Angiostatin Krlngle 1-3 by Actlvated Human Neutrophils THE JOURNAL OF IMMUNOLOGY (2002) 168 5798-5804

32 LEALI D BELLERI M URBINATI C COL TRINI D ORESTE P ZOPPETTI G RIBATTI D RUSNATI M PRESTA M Flbroblast Growth Factor -2 Antagon lst Aotivity and Angiostatic CapacitY of Sulfated Escherichia coli K5 Polysaccharide J BIOLCHEM (2001) 276 37900-37908

33 DELLERA P BELLERI M STABILE H IVIASSARDI ML RIBATTI D PRESTA M Paracrine and autocrine effects of Fibroblast growth factor-4 In endothelial cellsONCOGENE (2001) 20 2655-2663

34 RIBATTI D GUALANDRIS A BELLERI M MASSARDI L NICOL RUSNATI M DELLERA P VACCA A RONCALI L PRESTA M Alteratlons of blood vessel development by endothelial cells overexpresslng fibroblast growth factor-2 J PATHOL (1 999) 189 590-599

35 PRESTA M RUSNATI M BELLERI M MORBIDELLI L ZICHE M and RIBATTI D Purine analogue 6-methylmercaptopurlne rlboslde lnhibits early and late phases of the anglogenesis process CANCER RES (1999) 59 2417-2424

36 PRESTA M BELLERI M VECCHI A HESSELGESSER J MANTOVANI A HORUK R Non competitive chemokine-medlated lnhibition of basic fibroblast growth factor-lnduced endothelial celi proliferation J BIOL CHEM (1998) 273 7911-7919

37 SOLA F GUALANDRIS A BELLERI M GIULIANI R1 COLTRINI D BASTAKI M MOLINARI TOSATTI MP BONARDI F VECCHI A FIORETTI F GIAVAZZI R CIOMEI M GRANDI M MANTOVANI A PRESTA M Endothelial cells overexpressing basic fibroblast growth factor (FGF2) induce vascular tumors in lmmunodeficient mice ANGIOGENESIS (1997) 1 102-116

38 GUALANDRIS A RUSNATI M BELLERI M MOLINARl-TOSATTI MP BONARDI F PAROLINI S ALBINI A ZICHE M PRESTA M Angiogenic phenotype induced by basic fibroblast growth factor transfectlon in brain microvascu lar endothelial cells an in vitro autocrine model of angiogenesis in brain tumors INT J ONCOL (1996) 8 567-573

39 GUALANDRIS A RUSNATI M BELLERI M NELLI EE BASTAKI M MOLINARl-TOSATTI MP BONARDI F PAROLINI S ALBINI A MORBIDELLI L ZICHE M CORALLINI A POSSATI L VACCA A RIBATTI D PRESTA M Basic fibroblast growth factor overexpression in endothelial cells an autocrine mechanism for anglogenesis and ang loproliferative disease CELL GROWTH amp DIFFERENTIATION (1996) 7 147-160