jc synthetic biology 6-15-2012
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TRANSCRIPT
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Synthetic Biology
Diane Wu
Fire Lab Summer Journal Club
6/15/2012
http://www.moulicohen.com/2010/01/07/synthetic-biology-%E2%80%93-the-next-biotech-revolution/
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Some background
• DNA->RNA->Protein
http://bioweb.uwlax.edu/genweb/molecular/theory/transcription/transcription.htm
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Toolbox
• Zinc-finger
http://2010.igem.org/Team:Debrecen-Hungary/minimals http://en.wikipedia.org/wiki/File:Zinc_finger_DNA_complex.png
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Toolbox
• Zinc-finger• siRNA
http://2010.igem.org/Team:Debrecen-Hungary/minimals http://en.wikipedia.org/wiki/File:Zinc_finger_DNA_complex.png
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Toolbox
• Zinc-finger• siRNA• Recombinase
Alonso J C et al. J. Biol. Chem. 1995;270:2938-2945http://www.scholarpedia.org/article/Gene_assembly_in_Ciliates
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Toolbox
• Zinc-finger• siRNA• Recombinase• Ribosome Binding
Site (RBS)taRNA
cr
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http://www.bio.davidson.edu/Courses/Molbio/MolStudents/spring2003/Keogh/bacteriophage2.jpg
http://thet2phage.com/Art/bacteriophage.jpg
http://blass.com.au/definitions/coli%20phage http://bio1903.nicerweb.com/doc/class/bio1903/Locked/media/ch18/18_01T4PhageEColi_LP.jpg
http://bioweb.wku.edu/courses/biol566/L2lambda.html
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http://upload.wikimedia.org/wikipedia/commons/2/2f/Image-Bacteriophage_lambda_genome.png
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The toggle switch
Recall:
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The toggle switch
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Case study: Counters
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Case study: Counters
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Cre/FLPe
Cre recognizes loxPFLPe recognizes FRT
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Cre/FLPe
Cre recognizes loxPFLPe recognizes FRT
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Analog to Digital
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Oh, the possibilities!
• Whole-cell biosensors
• Microbial engineering using circuit carrying bacteriophages
• Biological containment
• iPSC control
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Issues of consideration
• Modularity
• Biological knowledge
• Probes to assay and characterize in vivo operation of synthetic gene circuits
http://estherspace.wordpress.com/2008/01/21/ http://naturalorder.info/
vs.
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References
• Friedland et al. Synthetic Gene Netowrks that Count. Science (2009)
• Lu, Khalil, and Collins. Next-generation synthetic gene networks. Nature Biotechnology (2009)
• Gardner, Canter, and Collins. Construction of a genetic toggle switch in Escherichia coli. Nature (2000)