jeffrey fontenot, reu student. to seal off any broken or leaking blood vessels protecting damaged...
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![Page 1: Jeffrey Fontenot, REU Student. To seal off any broken or leaking blood vessels Protecting damaged tissue while it heals](https://reader036.vdocument.in/reader036/viewer/2022062408/56649f2c5503460f94c4740c/html5/thumbnails/1.jpg)
Jeffrey Fontenot, REU Student
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To seal off any broken or leaking blood vessels
Protecting damaged tissue while it heals
http://biomed.brown.edu/Courses/BI108/BI108_2005_Groups/10/webpages/plateletslink.htm
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Over-activity◦ Clots and thrombi
form in unnecessary places or situations
◦ Thrombi may become larger than needed
◦ Heart attack and stroke may result
Under-activity◦ Clots do not form
when needed◦ Excessive bleeding◦ Wounds not healing
quickly or properly
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The end goal ◦ Develop processes and devices that
can measure platelet activity◦ Diagnose platelet problems or
dysfunction in the clinical setting Intermediate goal
◦ Show that L-arginine decreases platelet adhesion
◦ Show that higher shear stress will increase platelet adhesion
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Reproducing previous results◦ Positive control◦ Constant flow rate◦ Change L-arginine
concentrations from 0 to 25 µM/L
New work◦ Use different shear
stresses ranging from 1 to 15 dynes/cm2
◦ Use two different concentrations of L-arginine, 0 and 20 µM/L
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Cut out micro channels Layer-by-Layer self
assembly Manifold and syringe
pump Calculate flow rate from
the desired shear stress◦
Bovine blood is collected and spun down to separate the Platelet Rich Plasma (PRP) which settles on the top
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PRP travels down through the channel for one minute. The channels are rinsed with PBSEach channel is dyed with acridine orange
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Each channel is photographed 22 times
The images are processed through a MatLab code
The average percent coverage is calculated in Excel
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The positive control results
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The current work results
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Shear stress and L-arginine do have effects on platelet adhesion
Increasing shear stress appears to decrease adhesion◦ Possibly due to sodium citrate,
Yasuo et al. Source of errors
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Correct the errors The results appear to
be opposite of the expected
There are more factors than originally expected◦ The anticoagulant may
play a role◦ Is there a delay between
activation and adhesion◦ Is the thrombus forming
and breaking up
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Dr. Steven JonesMelanie GroanLouisiana Tech UniversityNational Science Foundation
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